AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1361910


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361910 MtrunA17_Chr7g0269961 95.312 128 5 1 1 127 1 128 3.00e-87 249
Msa1361910 MtrunA17_Chr1g0194671 64.615 130 41 3 2 127 3 131 4.43e-54 166
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1361910 AT3G62410.1 59.398 133 46 5 1 127 1 131 4.50e-45 143
Msa1361910 AT2G47400.1 68.041 97 27 2 33 127 30 124 4.40e-40 130
Msa1361910 AT1G76560.1 47.297 74 37 1 56 127 61 134 6.05e-19 77.0

Find 40 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCTTCTTTGATATCTGTTC+TGG 0.263996 tig0017690:-7928 None:intergenic
TGTTGTTGGTGAGATCTTGA+TGG 0.363034 tig0017690:-7836 None:intergenic
AAGCTTCTCTTCAACTCATC+AGG 0.395970 tig0017690:+7802 Msa1361910:CDS
GCTACGCATTCTCCACTAAC+CGG 0.412486 tig0017690:-7994 None:intergenic
GAAGAAGAAATGGCAACAAT+AGG 0.418272 tig0017690:+7757 None:intergenic
GATGAGTTGAAGAGAAGCTT+GGG 0.422285 tig0017690:-7799 None:intergenic
CACCATCATTCGACCTGATC+CGG 0.449625 tig0017690:-7884 None:intergenic
CGGTGGGGTGTAGCCGGATC+AGG 0.450264 tig0017690:+7871 Msa1361910:CDS
CGTGTCTGCAGTGGTGTTGT+TGG 0.454736 tig0017690:-7850 None:intergenic
GGTGAGATCTTGATGGTTTG+TGG 0.456742 tig0017690:-7829 None:intergenic
TGTTCTGGTGCAGCATGAAC+TGG 0.466575 tig0017690:-7913 None:intergenic
TGGAGAATGCGTAGCGGCAT+GGG 0.484341 tig0017690:+8002 Msa1361910:CDS
TGTGCTGATGACCCGGTTAG+TGG 0.494167 tig0017690:+7982 Msa1361910:CDS
GAAGAAGGATTCTGATCCAT+TGG 0.495582 tig0017690:+8071 Msa1361910:CDS
CGGCGAGTCATGCAAGGGAT+AGG 0.497910 tig0017690:+8049 Msa1361910:CDS
GTGGAGAATGCGTAGCGGCA+TGG 0.516210 tig0017690:+8001 Msa1361910:CDS
CAGACACGGTGGGGTGTAGC+CGG 0.517241 tig0017690:+7865 Msa1361910:CDS
TGATGAGTTGAAGAGAAGCT+TGG 0.520626 tig0017690:-7800 None:intergenic
AGAGAAGCTTGGGGTTTGAA+AGG 0.525586 tig0017690:-7789 None:intergenic
TCCATTGGAGGATTACTGTA+AGG 0.532128 tig0017690:+8086 Msa1361910:CDS
TCATGCAAGGGATAGGAAGA+AGG 0.572718 tig0017690:+8056 Msa1361910:CDS
ACCAGAACAGATATCAAAGA+AGG 0.574036 tig0017690:+7927 Msa1361910:CDS
AGAGACATGTGCTGATGACC+CGG 0.581174 tig0017690:+7975 Msa1361910:CDS
TCCTTACAGTAATCCTCCAA+TGG 0.581674 tig0017690:-8087 None:intergenic
AGCGGCATGGGACGAAGTGG+AGG 0.586526 tig0017690:+8014 Msa1361910:CDS
AGCCGGATCAGGTCGAATGA+TGG 0.587328 tig0017690:+7882 Msa1361910:CDS
CTACGCATTCTCCACTAACC+GGG 0.589171 tig0017690:-7993 None:intergenic
CAACAACACCACTGCAGACA+CGG 0.593172 tig0017690:+7851 Msa1361910:CDS
GAAGGATTCTGATCCATTGG+AGG 0.594880 tig0017690:+8074 Msa1361910:CDS
GAAGAAATGGCAACAATAGG+TGG 0.596013 tig0017690:+7760 None:intergenic
ACACCCCACCGTGTCTGCAG+TGG 0.598200 tig0017690:-7859 None:intergenic
AACACCACTGCAGACACGGT+GGG 0.598529 tig0017690:+7855 Msa1361910:CDS
CGTAGCGGCATGGGACGAAG+TGG 0.601693 tig0017690:+8011 Msa1361910:CDS
ACACCACTGCAGACACGGTG+GGG 0.612423 tig0017690:+7856 Msa1361910:CDS
CGGATCAGGTCGAATGATGG+TGG 0.612425 tig0017690:+7885 Msa1361910:CDS
TGCTGCGGCGAGTCATGCAA+GGG 0.625380 tig0017690:+8044 Msa1361910:CDS
CAACACCACTGCAGACACGG+TGG 0.673443 tig0017690:+7854 Msa1361910:CDS
ATGAGTTGAAGAGAAGCTTG+GGG 0.682917 tig0017690:-7798 None:intergenic
AGTGGAGGAACTCAGTGCTG+CGG 0.683881 tig0017690:+8029 Msa1361910:CDS
GGTTAGTGGAGAATGCGTAG+CGG 0.759059 tig0017690:+7996 Msa1361910:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACCAGAACAGATATCAAAGA+AGG + tig0017690:7927-7946 Msa1361910:CDS 35.0%
ACCTTCTTTGATATCTGTTC+TGG - tig0017690:7931-7950 None:intergenic 35.0%
! ATGAGTTGAAGAGAAGCTTG+GGG - tig0017690:7801-7820 None:intergenic 40.0%
! GATGAGTTGAAGAGAAGCTT+GGG - tig0017690:7802-7821 None:intergenic 40.0%
! TGATGAGTTGAAGAGAAGCT+TGG - tig0017690:7803-7822 None:intergenic 40.0%
AAGCTTCTCTTCAACTCATC+AGG + tig0017690:7802-7821 Msa1361910:CDS 40.0%
! TGTTGTTGGTGAGATCTTGA+TGG - tig0017690:7839-7858 None:intergenic 40.0%
GAAGAAGGATTCTGATCCAT+TGG + tig0017690:8071-8090 Msa1361910:CDS 40.0%
TCCATTGGAGGATTACTGTA+AGG + tig0017690:8086-8105 Msa1361910:CDS 40.0%
TCCTTACAGTAATCCTCCAA+TGG - tig0017690:8090-8109 None:intergenic 40.0%
! TTTTGCACTCATCAGTCTCA+GGG - tig0017690:8116-8135 None:intergenic 40.0%
AGAGAAGCTTGGGGTTTGAA+AGG - tig0017690:7792-7811 None:intergenic 45.0%
! GGTGAGATCTTGATGGTTTG+TGG - tig0017690:7832-7851 None:intergenic 45.0%
TCATGCAAGGGATAGGAAGA+AGG + tig0017690:8056-8075 Msa1361910:CDS 45.0%
GAAGGATTCTGATCCATTGG+AGG + tig0017690:8074-8093 Msa1361910:CDS 45.0%
! GTTTTGCACTCATCAGTCTC+AGG - tig0017690:8117-8136 None:intergenic 45.0%
CAACAACACCACTGCAGACA+CGG + tig0017690:7851-7870 Msa1361910:CDS 50.0%
CACCATCATTCGACCTGATC+CGG - tig0017690:7887-7906 None:intergenic 50.0%
TGTTCTGGTGCAGCATGAAC+TGG - tig0017690:7916-7935 None:intergenic 50.0%
! AGAGACATGTGCTGATGACC+CGG + tig0017690:7975-7994 Msa1361910:CDS 50.0%
CTACGCATTCTCCACTAACC+GGG - tig0017690:7996-8015 None:intergenic 50.0%
GCTACGCATTCTCCACTAAC+CGG - tig0017690:7997-8016 None:intergenic 50.0%
GGTTAGTGGAGAATGCGTAG+CGG + tig0017690:7996-8015 Msa1361910:CDS 50.0%
!! CGTGTCTGCAGTGGTGTTGT+TGG - tig0017690:7853-7872 None:intergenic 55.0%
AACACCACTGCAGACACGGT+GGG + tig0017690:7855-7874 Msa1361910:CDS 55.0%
AGCCGGATCAGGTCGAATGA+TGG + tig0017690:7882-7901 Msa1361910:CDS 55.0%
CGGATCAGGTCGAATGATGG+TGG + tig0017690:7885-7904 Msa1361910:CDS 55.0%
! TGTGCTGATGACCCGGTTAG+TGG + tig0017690:7982-8001 Msa1361910:CDS 55.0%
TGGAGAATGCGTAGCGGCAT+GGG + tig0017690:8002-8021 Msa1361910:CDS 55.0%
AGTGGAGGAACTCAGTGCTG+CGG + tig0017690:8029-8048 Msa1361910:CDS 55.0%
CAACACCACTGCAGACACGG+TGG + tig0017690:7854-7873 Msa1361910:CDS 60.0%
ACACCACTGCAGACACGGTG+GGG + tig0017690:7856-7875 Msa1361910:CDS 60.0%
GTGGAGAATGCGTAGCGGCA+TGG + tig0017690:8001-8020 Msa1361910:CDS 60.0%
TGCTGCGGCGAGTCATGCAA+GGG + tig0017690:8044-8063 Msa1361910:CDS 60.0%
CGGCGAGTCATGCAAGGGAT+AGG + tig0017690:8049-8068 Msa1361910:CDS 60.0%
ACACCCCACCGTGTCTGCAG+TGG - tig0017690:7862-7881 None:intergenic 65.0%
CAGACACGGTGGGGTGTAGC+CGG + tig0017690:7865-7884 Msa1361910:CDS 65.0%
CGTAGCGGCATGGGACGAAG+TGG + tig0017690:8011-8030 Msa1361910:CDS 65.0%
AGCGGCATGGGACGAAGTGG+AGG + tig0017690:8014-8033 Msa1361910:CDS 65.0%
GTGCTGCGGCGAGTCATGCA+AGG + tig0017690:8043-8062 Msa1361910:CDS 65.0%
CGGTGGGGTGTAGCCGGATC+AGG + tig0017690:7871-7890 Msa1361910:CDS 70.0%
Chromosome Type Strat End Strand Name
tig0017690 gene 7766 8149 7766 ID=Msa1361910;Name=Msa1361910
tig0017690 mRNA 7766 8149 7766 ID=Msa1361910-mRNA-1;Parent=Msa1361910;Name=Msa1361910-mRNA-1;_AED=0.01;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|127
tig0017690 exon 7766 8149 7766 ID=Msa1361910-mRNA-1:exon:12710;Parent=Msa1361910-mRNA-1
tig0017690 CDS 7766 8149 7766 ID=Msa1361910-mRNA-1:cds;Parent=Msa1361910-mRNA-1
Gene Sequence

>Msa1361910

ATGGCAACAATAGGTGGTCTCAGCCTTTCAAACCCCAAGCTTCTCTTCAACTCATCAGGATTTCCACAAACCATCAAGATCTCACCAACAACACCACTGCAGACACGGTGGGGTGTAGCCGGATCAGGTCGAATGATGGTGGTTCGACCAGTTCATGCTGCACCAGAACAGATATCAAAGAAGGTAGAAGAAAGCATTAAGAGTGCGCAAGAGACATGTGCTGATGACCCGGTTAGTGGAGAATGCGTAGCGGCATGGGACGAAGTGGAGGAACTCAGTGCTGCGGCGAGTCATGCAAGGGATAGGAAGAAGGATTCTGATCCATTGGAGGATTACTGTAAGGATAACCCTGAGACTGATGAGTGCAAAACTTATGATAACTGA

Protein sequence

>Msa1361910

MATIGGLSLSNPKLLFNSSGFPQTIKISPTTPLQTRWGVAGSGRMMVVRPVHAAPEQISKKVEESIKSAQETCADDPVSGECVAAWDEVEELSAAASHARDRKKDSDPLEDYCKDNPETDECKTYDN*