AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1456410


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456410 MtrunA17_Chr6g0484911 100.000 75 0 0 1 75 132 206 1.21e-51 159
Msa1456410 MtrunA17_Chr5g0422751 85.333 75 11 0 1 75 82 156 3.96e-43 136
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1456410 AT1G68660.1 86.667 75 10 0 1 75 85 159 1.87e-44 140
Msa1456410 AT1G68660.2 50.667 75 7 1 1 75 85 129 1.09e-17 71.6

Find 13 sgRNAs with CRISPR-Local

Find 12 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATGCAAGAGGCACATATTAA+TGG 0.315465 tig0037252:+1449 Msa1456410:CDS
GCAATATCAACTGTCATTCC+AGG 0.427583 tig0037252:-1416 Msa1456410:intergenic
TGCATGCAATTGAGAGGAAA+TGG 0.433575 tig0037252:+1518 Msa1456410:CDS
TTGTGTAGCATTACTCTGTA+TGG 0.492187 tig0037252:-1341 Msa1456410:intergenic
TTCCTCTCAATTGCATGCAA+TGG 0.502233 tig0037252:-1514 Msa1456410:intergenic
ACTCAACAACCACCATCAGC+TGG 0.533580 tig0037252:-1560 Msa1456410:intergenic
AGTTCAATTGAACCAGCTGA+TGG 0.567124 tig0037252:+1548 Msa1456410:CDS
TGCAGTTAACGTCATGCAAG+AGG 0.589593 tig0037252:+1436 Msa1456410:CDS
GACCATTGCATGCAATTGAG+AGG 0.614338 tig0037252:+1512 Msa1456410:CDS
GTGTTGATGAAAGTGATACC+TGG 0.634232 tig0037252:+1398 Msa1456410:CDS
TGTAGCATTACTCTGTATGG+TGG 0.656579 tig0037252:-1338 Msa1456410:intergenic
ACATATTAATGGATTAGCAG+TGG 0.658551 tig0037252:+1460 Msa1456410:CDS
TCAATTGAACCAGCTGATGG+TGG 0.677829 tig0037252:+1551 Msa1456410:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ACATATTAATGGATTAGCAG+TGG + tig0037252:1460-1479 Msa1456410:CDS 30.0%
TTGTGTAGCATTACTCTGTA+TGG - tig0037252:1344-1363 None:intergenic 35.0%
! ATGCAAGAGGCACATATTAA+TGG + tig0037252:1449-1468 Msa1456410:CDS 35.0%
TGTAGCATTACTCTGTATGG+TGG - tig0037252:1341-1360 None:intergenic 40.0%
! GTGTTGATGAAAGTGATACC+TGG + tig0037252:1398-1417 Msa1456410:CDS 40.0%
GCAATATCAACTGTCATTCC+AGG - tig0037252:1419-1438 Msa1456410:intergenic 40.0%
TTCCTCTCAATTGCATGCAA+TGG - tig0037252:1517-1536 Msa1456410:intergenic 40.0%
TGCATGCAATTGAGAGGAAA+TGG + tig0037252:1518-1537 Msa1456410:CDS 40.0%
AGTTCAATTGAACCAGCTGA+TGG + tig0037252:1548-1567 Msa1456410:CDS 40.0%
TGCAGTTAACGTCATGCAAG+AGG + tig0037252:1436-1455 Msa1456410:CDS 45.0%
GACCATTGCATGCAATTGAG+AGG + tig0037252:1512-1531 Msa1456410:CDS 45.0%
TCAATTGAACCAGCTGATGG+TGG + tig0037252:1551-1570 Msa1456410:CDS 45.0%
Chromosome Type Strat End Strand Name
tig0037252 gene 1319 1577 1319 ID=Msa1456410;Name=Msa1456410
tig0037252 mRNA 1319 1577 1319 ID=Msa1456410-mRNA-1;Parent=Msa1456410;Name=Msa1456410-mRNA-1;_AED=0.02;_eAED=0.15;_QI=34|-1|0|1|-1|0|1|0|75
tig0037252 exon 1319 1577 1319 ID=Msa1456410-mRNA-1:exon:20709;Parent=Msa1456410-mRNA-1
tig0037252 five_prime_UTR 1319 1352 1319 ID=Msa1456410-mRNA-1:five_prime_utr;Parent=Msa1456410-mRNA-1
tig0037252 CDS 1353 1577 1353 ID=Msa1456410-mRNA-1:cds;Parent=Msa1456410-mRNA-1
Gene Sequence

>Msa1456410

ATGCTACACAATGACAACTATAACAAAAGAGAATATGTAGTGCAAGTGTTGATGAAAGTGATACCTGGAATGACAGTTGATATTGCAGTTAACGTCATGCAAGAGGCACATATTAATGGATTAGCAGTGGTCATTATTTGTGCTCAAGTTGATGCTGAAGACCATTGCATGCAATTGAGAGGAAATGGTCTTTTAAGTTCAATTGAACCAGCTGATGGTGGTTGT

Protein sequence

>Msa1456410

MLHNDNYNKREYVVQVLMKVIPGMTVDIAVNVMQEAHINGLAVVIICAQVDAEDHCMQLRGNGLLSSIEPADGGC