AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0649220


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649220 MtrunA17_Chr4g0040991 100.000 77 0 0 1 77 1 77 2.07e-48 147
Msa0649220 MtrunA17_Chr2g0325441 89.610 77 8 0 1 77 1 77 1.42e-45 140
Msa0649220 MtrunA17_Chr2g0283451 75.000 76 19 0 2 77 5 80 4.57e-35 114
Msa0649220 MtrunA17_Chr2g0324911 58.974 78 31 1 1 77 1 78 6.57e-21 78.2
Msa0649220 MtrunA17_Chr4g0040491 53.521 71 33 0 7 77 9 79 2.80e-14 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0649220 AT2G31090.1 76.712 73 17 0 5 77 3 75 4.80e-29 99.0
Msa0649220 AT2G20562.1 52.778 72 34 0 6 77 2 73 2.97e-21 79.3

Find 17 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCGTTGGTGTACTCATC+TGG 0.378938 4_3:-55805920 Msa0649220:CDS
CTCTCAGCAGGGGATCTTAG+AGG 0.383799 4_3:+55805679 None:intergenic
GGTGTACTCATCTGGATTGT+CGG 0.396564 4_3:-55805912 Msa0649220:CDS
GAAGGTGAATGCAGACCATT+GGG 0.445910 4_3:-55805987 Msa0649220:CDS
TGTGGAACTTGCGCTTGAAT+TGG 0.449919 4_3:-55805735 Msa0649220:CDS
AGAAGGTGAATGCAGACCAT+TGG 0.487199 4_3:-55805988 Msa0649220:CDS
GTGTACTCATCTGGATTGTC+GGG 0.488369 4_3:-55805911 Msa0649220:intron
TTTATGCTTAACTATAATTG+TGG 0.504363 4_3:-55805753 Msa0649220:CDS
TGTCTTCTCATCTCAATCGT+TGG 0.507454 4_3:-55805933 Msa0649220:CDS
CAAAGCTCCATTGCATGTCA+TGG 0.535851 4_3:-55805711 Msa0649220:CDS
AACCACTCCATGACATGCAA+TGG 0.552057 4_3:+55805704 None:intergenic
GAAAAGATGTGCAATTCAGA+AGG 0.574414 4_3:-55806005 Msa0649220:exon
AGAAGACAGAGAAAAGCGAA+AGG 0.608508 4_3:+55805948 None:intergenic
CTCCATTGCATGTCATGGAG+TGG 0.615369 4_3:-55805706 Msa0649220:CDS
ATAGTTAAGCATAAACAACA+AGG 0.675018 4_3:+55805761 None:intergenic
AGACCTAATAGAAAACCCAA+TGG 0.675649 4_3:+55805972 None:intergenic
TAGTTAAGCATAAACAACAA+GGG 0.709299 4_3:+55805762 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTATTCATATTTTGCAGAT+TGG - chr4_3:55805821-55805840 Msa0649220:intron 20.0%
!! TTTATGCTTAACTATAATTG+TGG - chr4_3:55805869-55805888 Msa0649220:intron 20.0%
! TGATTTCTTCATATTTGATC+TGG - chr4_3:55805766-55805785 Msa0649220:CDS 25.0%
! GATTTCTTCATATTTGATCT+GGG - chr4_3:55805767-55805786 Msa0649220:CDS 25.0%
! TAGTTAAGCATAAACAACAA+GGG + chr4_3:55805863-55805882 None:intergenic 25.0%
! ATAGTTAAGCATAAACAACA+AGG + chr4_3:55805864-55805883 None:intergenic 25.0%
! AAAAAAAAAGTCAAAGTCCA+AGG + chr4_3:55806031-55806050 None:intergenic 25.0%
GAAAAGATGTGCAATTCAGA+AGG - chr4_3:55805617-55805636 Msa0649220:three_prime_UTR 35.0%
! AGACCATTGGGTTTTCTATT+AGG - chr4_3:55805647-55805666 Msa0649220:three_prime_UTR 35.0%
AGACCTAATAGAAAACCCAA+TGG + chr4_3:55805653-55805672 None:intergenic 35.0%
AGAAGACAGAGAAAAGCGAA+AGG + chr4_3:55805677-55805696 None:intergenic 40.0%
TGTCTTCTCATCTCAATCGT+TGG - chr4_3:55805689-55805708 Msa0649220:CDS 40.0%
AATTACTCTTCCCACTCTTC+TGG - chr4_3:55805736-55805755 Msa0649220:CDS 40.0%
ATTACTCTTCCCACTCTTCT+GGG - chr4_3:55805737-55805756 Msa0649220:CDS 40.0%
TCAAAGTAACCCAGAAGAGT+GGG + chr4_3:55805749-55805768 None:intergenic 40.0%
ATCAAAGTAACCCAGAAGAG+TGG + chr4_3:55805750-55805769 None:intergenic 40.0%
AGAAGGTGAATGCAGACCAT+TGG - chr4_3:55805634-55805653 Msa0649220:three_prime_UTR 45.0%
GAAGGTGAATGCAGACCATT+GGG - chr4_3:55805635-55805654 Msa0649220:three_prime_UTR 45.0%
CAATCGTTGGTGTACTCATC+TGG - chr4_3:55805702-55805721 Msa0649220:CDS 45.0%
GGTGTACTCATCTGGATTGT+CGG - chr4_3:55805710-55805729 Msa0649220:CDS 45.0%
GTGTACTCATCTGGATTGTC+GGG - chr4_3:55805711-55805730 Msa0649220:CDS 45.0%
TGTGGAACTTGCGCTTGAAT+TGG - chr4_3:55805887-55805906 Msa0649220:intron 45.0%
CAAAGCTCCATTGCATGTCA+TGG - chr4_3:55805911-55805930 Msa0649220:intron 45.0%
AACCACTCCATGACATGCAA+TGG + chr4_3:55805921-55805940 None:intergenic 45.0%
CTCCATTGCATGTCATGGAG+TGG - chr4_3:55805916-55805935 Msa0649220:CDS 50.0%
TCTCTCTCTCTCTCTCAGCA+GGG + chr4_3:55805957-55805976 None:intergenic 50.0%
GAGAGAGAGAGAGAGTTCCT+TGG - chr4_3:55806011-55806030 Msa0649220:exon 50.0%
CTCTCAGCAGGGGATCTTAG+AGG + chr4_3:55805946-55805965 None:intergenic 55.0%
CTCTCTCTCTCTCTCAGCAG+GGG + chr4_3:55805956-55805975 None:intergenic 55.0%
CTCTCTCTCTCTCTCTCAGC+AGG + chr4_3:55805958-55805977 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
chr4_3 gene 55805589 55806055 55805589 ID=Msa0649220;Name=Msa0649220
chr4_3 mRNA 55805589 55806055 55805589 ID=Msa0649220-mRNA-1;Parent=Msa0649220;Name=Msa0649220-mRNA-1;_AED=0.00;_eAED=0.00;_QI=34|1|1|1|1|1|2|93|77
chr4_3 exon 55805589 55805805 55805589 ID=Msa0649220-mRNA-1:exon:14391;Parent=Msa0649220-mRNA-1
chr4_3 exon 55805912 55806055 55805912 ID=Msa0649220-mRNA-1:exon:14390;Parent=Msa0649220-mRNA-1
chr4_3 five_prime_UTR 55806022 55806055 55806022 ID=Msa0649220-mRNA-1:five_prime_utr;Parent=Msa0649220-mRNA-1
chr4_3 CDS 55805912 55806021 55805912 ID=Msa0649220-mRNA-1:cds;Parent=Msa0649220-mRNA-1
chr4_3 CDS 55805682 55805805 55805682 ID=Msa0649220-mRNA-1:cds;Parent=Msa0649220-mRNA-1
chr4_3 three_prime_UTR 55805589 55805681 55805589 ID=Msa0649220-mRNA-1:three_prime_utr;Parent=Msa0649220-mRNA-1
Gene Sequence

>Msa0649220

ATGTGCAATTCAGAAGGTGAATGCAGACCATTGGGTTTTCTATTAGGTCTTCCTTTCGCTTTTCTCTGTCTTCTCATCTCAATCGTTGGTGTACTCATCTGGATTGTCGGATTGGCGCTTACTTGTATATGCCCTTGTTGTTTATGCTTAACTATAATTGTGGAACTTGCGCTTGAATTGGTCAAAGCTCCATTGCATGTCATGGAGTGGTTCACCTCTAAGATCCCCTGCTGA

Protein sequence

>Msa0649220

MCNSEGECRPLGFLLGLPFAFLCLLISIVGVLIWIVGLALTCICPCCLCLTIIVELALELVKAPLHVMEWFTSKIPC*