AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene009400

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009400.t1 MTR_3g007740 83.747 443 27 2 1 398 1 443 0.0 727
MS.gene009400.t1 MTR_7g063670 58.134 461 118 7 1 398 4 452 1.05e-169 483
MS.gene009400.t1 MTR_7g062300 49.790 476 144 13 5 396 8 472 1.10e-130 384
MS.gene009400.t1 MTR_7g062280 47.746 488 154 11 5 398 6 486 1.83e-130 384
MS.gene009400.t1 MTR_7g063840 49.263 475 157 10 1 398 1 468 1.59e-128 378
MS.gene009400.t1 MTR_7g063830 48.352 455 153 8 5 398 6 439 6.32e-126 370
MS.gene009400.t1 MTR_7g063920 51.577 444 146 11 5 398 7 431 2.44e-125 369
MS.gene009400.t1 MTR_7g063820 48.233 481 155 12 5 398 6 479 1.84e-122 363
MS.gene009400.t1 MTR_7g063660 48.414 473 156 12 7 398 7 472 2.22e-119 355
MS.gene009400.t1 MTR_7g063880 47.689 476 148 13 5 398 7 463 1.65e-117 350
MS.gene009400.t1 MTR_7g063900 46.939 490 158 14 2 398 3 483 1.07e-112 338
MS.gene009400.t1 MTR_7g063890 45.808 489 166 12 1 398 19 499 9.49e-100 306
MS.gene009400.t1 MTR_7g066480 40.046 437 185 13 9 398 16 422 1.34e-79 251
MS.gene009400.t1 MTR_7g066570 38.508 496 190 15 1 397 1 480 1.75e-79 255
MS.gene009400.t1 MTR_7g066260 36.876 461 208 12 8 397 14 462 9.72e-74 238
MS.gene009400.t1 MTR_7g066410 33.398 512 225 11 2 398 3 513 1.55e-71 233
MS.gene009400.t1 MTR_7g066340 36.771 446 223 12 8 397 11 453 5.84e-71 230
MS.gene009400.t1 MTR_7g066430 34.765 489 212 12 9 397 4 485 2.20e-69 227
MS.gene009400.t1 MTR_7g063690 53.571 252 76 6 1 243 3 222 2.31e-69 219
MS.gene009400.t1 MTR_7g012680 38.925 465 177 19 8 397 13 445 1.98e-67 221
MS.gene009400.t1 MTR_7g066490 37.123 431 192 11 9 398 16 408 2.24e-67 219
MS.gene009400.t1 MTR_7g066350 36.018 447 230 10 1 393 1 445 4.01e-65 215
MS.gene009400.t1 MTR_7g066580 37.665 454 197 18 15 394 1 442 1.34e-64 213
MS.gene009400.t1 MTR_3g065200 37.174 460 151 15 9 384 3 408 1.72e-64 213
MS.gene009400.t1 MTR_7g058710 34.161 483 211 14 8 398 11 478 1.12e-62 209
MS.gene009400.t1 MTR_7g066290 36.364 451 203 12 4 397 10 433 1.32e-62 207
MS.gene009400.t1 MTR_7g066400 33.713 439 220 10 9 393 12 433 6.43e-59 198
MS.gene009400.t1 MTR_7g058700 35.698 437 183 13 18 398 528 922 4.47e-58 204
MS.gene009400.t1 MTR_7g066360 35.345 464 202 16 5 394 7 446 8.79e-56 191
MS.gene009400.t1 MTR_7g066310 33.971 418 208 12 8 397 6 383 1.08e-55 189
MS.gene009400.t1 MTR_1g041315 33.261 463 205 14 3 381 9 451 3.40e-54 187
MS.gene009400.t1 MTR_4g019310 33.333 492 220 17 7 397 6 490 2.02e-52 182
MS.gene009400.t1 MTR_2g029400 33.557 447 190 14 2 387 5 405 9.31e-51 176
MS.gene009400.t1 MTR_7g063850 37.582 306 124 9 137 398 1 283 2.26e-43 153
MS.gene009400.t1 MTR_7g063720 42.308 234 102 6 190 398 2 227 5.11e-43 150
MS.gene009400.t1 MTR_7g063720 26.201 229 119 8 9 201 23 237 8.89e-11 62.0
MS.gene009400.t1 MTR_4g017410 28.713 505 233 14 1 398 1 485 9.42e-43 156
MS.gene009400.t1 MTR_4g017470 29.375 480 248 13 1 398 1 471 4.57e-41 152
MS.gene009400.t1 MTR_7g062270 45.161 186 53 5 1 140 1 183 1.46e-38 142
MS.gene009400.t1 MTR_4g017830 31.420 331 170 8 1 277 1 328 1.20e-35 137
MS.gene009400.t1 MTR_8g023240 25.885 452 255 12 10 389 38 481 1.02e-34 135
MS.gene009400.t1 MTR_4g017440 26.800 500 242 11 10 397 15 502 5.78e-33 129
MS.gene009400.t1 MTR_4g019340 27.660 470 247 12 6 385 35 501 6.63e-32 126
MS.gene009400.t1 MTR_4g017610 26.981 467 249 14 12 393 16 475 9.10e-32 125
MS.gene009400.t1 MTR_7g066680 34.323 303 126 7 163 393 33 334 3.07e-31 122
MS.gene009400.t1 MTR_7g100420 34.727 311 145 9 124 393 100 393 5.58e-31 122
MS.gene009400.t1 MTR_7g100420 76.087 46 11 0 7 52 37 82 7.14e-14 73.2
MS.gene009400.t1 MTR_7g063870 41.176 170 61 3 5 174 27 157 2.33e-29 115
MS.gene009400.t1 MTR_1g014060 37.561 205 82 6 22 182 13 215 3.65e-29 117
MS.gene009400.t1 MTR_7g063760 67.647 68 19 1 334 398 1 68 1.05e-23 94.0
MS.gene009400.t1 MTR_4g017550 24.561 456 240 14 39 395 21 471 2.77e-18 86.7
MS.gene009400.t1 MTR_8g445860 30.172 232 106 9 9 190 13 238 2.19e-17 81.3
MS.gene009400.t1 MTR_7g066280 28.340 247 124 7 77 292 2 226 2.07e-15 77.8
MS.gene009400.t1 MTR_1g115455 28.571 238 117 8 1 190 1 233 2.31e-14 72.4
MS.gene009400.t1 MTR_2g067310 37.500 128 69 3 21 141 59 182 4.30e-14 72.0
MS.gene009400.t1 MTR_1g115450 44.595 74 33 2 9 79 7 75 3.44e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene009400.t1 AT5G01130 31.957 460 234 16 1 397 1 444 9.37e-45 160
MS.gene009400.t1 AT3G09140 29.600 500 244 15 1 398 1 494 9.35e-42 154
MS.gene009400.t1 AT5G01120 30.271 479 236 14 10 393 10 485 1.20e-41 154
MS.gene009400.t1 AT3G09110 33.123 317 165 6 9 281 9 322 2.76e-41 149
MS.gene009400.t1 AT3G09140 28.220 528 243 16 1 398 1 522 8.09e-38 143
MS.gene009400.t1 AT5G43240 28.247 485 242 10 10 393 10 489 2.51e-37 142
MS.gene009400.t1 AT5G43240 28.247 485 242 10 10 393 10 489 2.51e-37 142
MS.gene009400.t1 AT5G43240 28.247 485 242 10 10 393 10 489 2.51e-37 142
MS.gene009400.t1 AT5G01150 27.419 496 246 13 1 398 1 480 1.82e-35 136
MS.gene009400.t1 AT3G09120 32.492 317 152 8 8 270 5 313 1.17e-34 130
MS.gene009400.t1 AT5G01140 26.915 483 235 17 1 397 1 451 5.57e-29 118
MS.gene009400.t1 AT5G37320 25.941 478 258 15 10 398 10 480 2.42e-24 104
MS.gene009400.t1 AT5G43240 27.812 320 178 7 122 393 31 345 2.84e-15 77.0

Find 61 sgRNAs with CRISPR-Local

Find 94 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATTGGATCTGCTCAAGAATT+TGG 0.125882 3.4:-3358679 MS.gene009400:CDS
CAAAACTCCTCTTGATGATT+TGG 0.207607 3.4:-3357842 MS.gene009400:CDS
TTAGTACCTATGCTCAAATA+AGG 0.230784 3.4:+3358843 None:intergenic
CTCCATCGATCACACGTTAT+TGG 0.237075 3.4:-3358697 MS.gene009400:CDS
AAGCCTGTTCTTTGCTTCTT+TGG 0.246726 3.4:+3358111 None:intergenic
GCAGAGTTACAGCATATTAA+AGG 0.249038 3.4:-3357679 MS.gene009400:intron
TGCAAATATAAAGACGAAAT+TGG 0.271766 3.4:+3357874 None:intergenic
AATTATCTAGGTAGTATTAA+TGG 0.291522 3.4:-3358181 MS.gene009400:CDS
TGTTCAGAAAGATTCAAATA+TGG 0.300824 3.4:-3359036 MS.gene009400:CDS
GCAAGTGTCTTTGAAACTTC+TGG 0.305904 3.4:-3359162 MS.gene009400:CDS
TATCAAAGTGTCGAAAATAT+TGG 0.330799 3.4:-3358981 MS.gene009400:CDS
GCAATGATGGCCATGATCTT+TGG 0.343011 3.4:+3358866 None:intergenic
TTCAGCAAATAAAACTTTGT+TGG 0.347611 3.4:+3359127 None:intergenic
ATCATTGATCGCTCAATAGA+TGG 0.350824 3.4:+3358394 None:intergenic
TTGGATCTGCTCAAGAATTT+GGG 0.352496 3.4:-3358678 MS.gene009400:CDS
TCTTCGGATGAATTATTAAT+CGG 0.359635 3.4:+3358951 None:intergenic
TTTACAAAAGTGATGAAGAT+TGG 0.383530 3.4:-3358012 MS.gene009400:CDS
GAACACATGAAGTAATTTGT+AGG 0.406683 3.4:+3358888 None:intergenic
GTTCAGAAAGATTCAAATAT+GGG 0.407299 3.4:-3359035 MS.gene009400:CDS
TAGTACCTATGCTCAAATAA+GGG 0.412015 3.4:+3358844 None:intergenic
GAGAGTGCTGCAATAATCTT+CGG 0.419427 3.4:+3358935 None:intergenic
AGCTTCTTAACCTTACCATT+AGG 0.423122 3.4:-3359074 MS.gene009400:CDS
AGTCTTGCAATTGTACCTAA+TGG 0.432976 3.4:+3359059 None:intergenic
TGTCAATTTCACTAAGGAAA+AGG 0.433062 3.4:-3358622 MS.gene009400:intron
AACTCCTCTTGATGATTTGG+AGG 0.435595 3.4:-3357839 MS.gene009400:CDS
TTGAGACAACCAATTGTAAC+TGG 0.442101 3.4:+3359011 None:intergenic
CTTCCAAAGAAGCAAAGAAC+AGG 0.450327 3.4:-3358114 MS.gene009400:CDS
TGCTCAAATAAGGGCAATGA+TGG 0.455804 3.4:+3358853 None:intergenic
TTTCTAACCTTTCCACTTGG+TGG 0.456804 3.4:-3358229 MS.gene009400:CDS
AATATGGGACCAGTTACAAT+TGG 0.460101 3.4:-3359020 MS.gene009400:CDS
CAAATCGGTTAGAGGTGTCT+TGG 0.469566 3.4:+3358432 None:intergenic
TTACAAGGGAATAATTATCT+AGG 0.478296 3.4:-3358193 MS.gene009400:CDS
ACATGAATAATTCTGTCATC+AGG 0.482964 3.4:+3358040 None:intergenic
TTCATGTGTTCCAAAGATCA+TGG 0.483746 3.4:-3358876 MS.gene009400:CDS
CATTGCCCTTATTTGAGCAT+AGG 0.493198 3.4:-3358849 MS.gene009400:intron
ACAATATATTGTATGCTCTA+GGG 0.496883 3.4:-3358282 MS.gene009400:CDS
TCAACATCTGCTCTCACAAA+TGG 0.498033 3.4:-3357645 MS.gene009400:CDS
TGCAATTGTACCTAATGGTA+AGG 0.498079 3.4:+3359064 None:intergenic
GTGATGAAGATTGGAGTGGT+GGG 0.499979 3.4:-3358003 MS.gene009400:CDS
AACACATGAAGTAATTTGTA+GGG 0.515631 3.4:+3358889 None:intergenic
GACAATATATTGTATGCTCT+AGG 0.518112 3.4:-3358283 MS.gene009400:CDS
GATGAAGATTGGAGTGGTGG+GGG 0.518998 3.4:-3358001 MS.gene009400:CDS
ATGGAACTAGTTACAGATGA+AGG 0.524395 3.4:-3357965 MS.gene009400:intron
CAATATATTGTATGCTCTAG+GGG 0.541114 3.4:-3358281 MS.gene009400:CDS
AGTGATGAAGATTGGAGTGG+TGG 0.550802 3.4:-3358004 MS.gene009400:CDS
ATCCAATAACGTGTGATCGA+TGG 0.554049 3.4:+3358695 None:intergenic
TGATGAAGATTGGAGTGGTG+GGG 0.563527 3.4:-3358002 MS.gene009400:CDS
AATGACTGTCAATTTCACTA+AGG 0.566839 3.4:-3358628 MS.gene009400:CDS
TCAAATCACCCTGAAGTTGG+TGG 0.578083 3.4:-3358320 MS.gene009400:CDS
TAAAACTCCACCAAGTGGAA+AGG 0.598205 3.4:+3358222 None:intergenic
TAGAGGTGTCTTGGAGAGCA+AGG 0.603942 3.4:+3358441 None:intergenic
CTACCTAAAACTCCACCAAG+TGG 0.611826 3.4:+3358217 None:intergenic
TGTTCAAATCACCCTGAAGT+TGG 0.614644 3.4:-3358323 MS.gene009400:CDS
ATATATTGTATGCTCTAGGG+GGG 0.624648 3.4:-3358279 MS.gene009400:CDS
TGTCACTATTGGCGTCGACG+AGG 0.634204 3.4:-3357809 MS.gene009400:intron
TATATTGTATGCTCTAGGGG+GGG 0.646973 3.4:-3358278 MS.gene009400:CDS
AACGTGTGATCGATGGAGTG+AGG 0.652725 3.4:+3358702 None:intergenic
AATATATTGTATGCTCTAGG+GGG 0.653820 3.4:-3358280 MS.gene009400:CDS
GACGAAATTGGAGACAACCA+TGG 0.679732 3.4:+3357886 None:intergenic
AAAAGTGATGAAGATTGGAG+TGG 0.722678 3.4:-3358007 MS.gene009400:CDS
ATGATCTGGTCATAGAACCA+TGG 0.740263 3.4:-3357903 MS.gene009400:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAGGTACTAAAAATATTTA+CGG - chr3.4:3357977-3357996 MS.gene009400:CDS 15.0%
!!! ACTATATGTATTTTTACTAT+GGG - chr3.4:3359063-3359082 MS.gene009400:CDS 15.0%
!! AATTATCTAGGTAGTATTAA+TGG - chr3.4:3358627-3358646 MS.gene009400:CDS 20.0%
!! AGTAAAAATACATATAGTGT+AGG + chr3.4:3359061-3359080 None:intergenic 20.0%
!! GAAAAATAAAAGTTCAAAAG+TGG + chr3.4:3358282-3358301 None:intergenic 20.0%
!!! CACTATATGTATTTTTACTA+TGG - chr3.4:3359062-3359081 MS.gene009400:CDS 20.0%
! ACAATATATTGTATGCTCTA+GGG - chr3.4:3358526-3358545 MS.gene009400:intron 25.0%
! ATCACAAATGTATTTGGTTA+CGG - chr3.4:3358878-3358897 MS.gene009400:CDS 25.0%
! CAAAAGATCACAAATGTATT+TGG - chr3.4:3358872-3358891 MS.gene009400:CDS 25.0%
! GAAACTAAGAACAATGATTT+AGG + chr3.4:3358239-3358258 None:intergenic 25.0%
! GTACTAAAAATATTTACGGT+AGG - chr3.4:3357981-3358000 MS.gene009400:CDS 25.0%
! GTTCAGAAAGATTCAAATAT+GGG - chr3.4:3357773-3357792 MS.gene009400:intron 25.0%
! TCTTCGGATGAATTATTAAT+CGG + chr3.4:3357860-3357879 None:intergenic 25.0%
! TGCAAATATAAAGACGAAAT+TGG + chr3.4:3358937-3358956 None:intergenic 25.0%
! TGTTCAGAAAGATTCAAATA+TGG - chr3.4:3357772-3357791 MS.gene009400:intron 25.0%
! TTACAAGGGAATAATTATCT+AGG - chr3.4:3358615-3358634 MS.gene009400:intron 25.0%
! TTCAGCAAATAAAACTTTGT+TGG + chr3.4:3357684-3357703 None:intergenic 25.0%
! TTTACAAAAGTGATGAAGAT+TGG - chr3.4:3358796-3358815 MS.gene009400:intron 25.0%
! TTTCTTTCCCAAAAACAAAT+CGG + chr3.4:3358394-3358413 None:intergenic 25.0%
!! AACACATGAAGTAATTTGTA+GGG + chr3.4:3357922-3357941 None:intergenic 25.0%
!!! TATCAAAGTGTCGAAAATAT+TGG - chr3.4:3357827-3357846 MS.gene009400:CDS 25.0%
AAAAATATTTACGGTAGGTG+TGG - chr3.4:3357986-3358005 MS.gene009400:CDS 30.0%
AATGACTGTCAATTTCACTA+AGG - chr3.4:3358180-3358199 MS.gene009400:CDS 30.0%
ACATGAATAATTCTGTCATC+AGG + chr3.4:3358771-3358790 None:intergenic 30.0%
CAATATATTGTATGCTCTAG+GGG - chr3.4:3358527-3358546 MS.gene009400:intron 30.0%
GACAATATATTGTATGCTCT+AGG - chr3.4:3358525-3358544 MS.gene009400:intron 30.0%
GATTAGTACTAATTCTGTAC+AGG - chr3.4:3358323-3358342 MS.gene009400:CDS 30.0%
TACTAATTCTGTACAGGTAT+TGG - chr3.4:3358329-3358348 MS.gene009400:CDS 30.0%
TAGTACCTATGCTCAAATAA+GGG + chr3.4:3357967-3357986 None:intergenic 30.0%
TGTCAATTTCACTAAGGAAA+AGG - chr3.4:3358186-3358205 MS.gene009400:CDS 30.0%
TTAGTACCTATGCTCAAATA+AGG + chr3.4:3357968-3357987 None:intergenic 30.0%
! AATATATTGTATGCTCTAGG+GGG - chr3.4:3358528-3358547 MS.gene009400:intron 30.0%
! GAACACATGAAGTAATTTGT+AGG + chr3.4:3357923-3357942 None:intergenic 30.0%
!! TTGTGCAATGGATTTGTTAA+AGG - chr3.4:3358043-3358062 MS.gene009400:CDS 30.0%
!!! AAAGTTTTATTTGCTGAAGC+AGG - chr3.4:3357686-3357705 MS.gene009400:intron 30.0%
AAAAGTGATGAAGATTGGAG+TGG - chr3.4:3358801-3358820 MS.gene009400:intron 35.0%
AATATGGGACCAGTTACAAT+TGG - chr3.4:3357788-3357807 MS.gene009400:intron 35.0%
AGCTTCTTAACCTTACCATT+AGG - chr3.4:3357734-3357753 MS.gene009400:intron 35.0%
AGTCTTGCAATTGTACCTAA+TGG + chr3.4:3357752-3357771 None:intergenic 35.0%
ATCATTGATCGCTCAATAGA+TGG + chr3.4:3358417-3358436 None:intergenic 35.0%
ATGGAACTAGTTACAGATGA+AGG - chr3.4:3358843-3358862 MS.gene009400:intron 35.0%
ATTGGATCTGCTCAAGAATT+TGG - chr3.4:3358129-3358148 MS.gene009400:CDS 35.0%
CAAATGTATTTGGTTACGGA+TGG - chr3.4:3358882-3358901 MS.gene009400:CDS 35.0%
GAGGAAAAAGTTGTCACTAT+TGG - chr3.4:3358988-3359007 MS.gene009400:CDS 35.0%
GCAGAGTTACAGCATATTAA+AGG - chr3.4:3359129-3359148 MS.gene009400:CDS 35.0%
TGCAATTGTACCTAATGGTA+AGG + chr3.4:3357747-3357766 None:intergenic 35.0%
TTCATGTGTTCCAAAGATCA+TGG - chr3.4:3357932-3357951 MS.gene009400:intron 35.0%
TTGAGACAACCAATTGTAAC+TGG + chr3.4:3357800-3357819 None:intergenic 35.0%
TTGGATCTGCTCAAGAATTT+GGG - chr3.4:3358130-3358149 MS.gene009400:CDS 35.0%
! ATTTTCTTACCACCAACTTC+AGG + chr3.4:3358500-3358519 None:intergenic 35.0%
! CAAAACTCCTCTTGATGATT+TGG - chr3.4:3358966-3358985 MS.gene009400:CDS 35.0%
! GATTTTCTAACCTTTCCACT+TGG - chr3.4:3358576-3358595 MS.gene009400:intron 35.0%
! TTTTCCTCCAAATCATCAAG+AGG + chr3.4:3358976-3358995 None:intergenic 35.0%
! TTTTCTTACCACCAACTTCA+GGG + chr3.4:3358499-3358518 None:intergenic 35.0%
!! ATATATTGTATGCTCTAGGG+GGG - chr3.4:3358529-3358548 MS.gene009400:intron 35.0%
!!! ACCTCTAACCGATTTGTTTT+TGG - chr3.4:3358383-3358402 MS.gene009400:CDS 35.0%
!!! CCTCTAACCGATTTGTTTTT+GGG - chr3.4:3358384-3358403 MS.gene009400:CDS 35.0%
!!! GAGTTTTAGGTAGGTTACAA+GGG - chr3.4:3358601-3358620 MS.gene009400:intron 35.0%
!!! TGGGGGAACTTTTTCAAAAA+TGG - chr3.4:3358824-3358843 MS.gene009400:intron 35.0%
AAGCCTGTTCTTTGCTTCTT+TGG + chr3.4:3358700-3358719 None:intergenic 40.0%
ATCCAATAACGTGTGATCGA+TGG + chr3.4:3358116-3358135 None:intergenic 40.0%
ATGATCTGGTCATAGAACCA+TGG - chr3.4:3358905-3358924 MS.gene009400:CDS 40.0%
CCCAAAAACAAATCGGTTAG+AGG + chr3.4:3358387-3358406 None:intergenic 40.0%
CTTCCAAAGAAGCAAAGAAC+AGG - chr3.4:3358694-3358713 MS.gene009400:CDS 40.0%
TAAAACTCCACCAAGTGGAA+AGG + chr3.4:3358589-3358608 None:intergenic 40.0%
TCAACATCTGCTCTCACAAA+TGG - chr3.4:3359163-3359182 MS.gene009400:CDS 40.0%
TGCTCAAATAAGGGCAATGA+TGG + chr3.4:3357958-3357977 None:intergenic 40.0%
TGTTCAAATCACCCTGAAGT+TGG - chr3.4:3358485-3358504 MS.gene009400:intron 40.0%
TTTCTAACCTTTCCACTTGG+TGG - chr3.4:3358579-3358598 MS.gene009400:intron 40.0%
! AACTCCTCTTGATGATTTGG+AGG - chr3.4:3358969-3358988 MS.gene009400:CDS 40.0%
! CATTGCCCTTATTTGAGCAT+AGG - chr3.4:3357959-3357978 MS.gene009400:intron 40.0%
! TGAAGCAGGCAAAGATTTTG+TGG - chr3.4:3357700-3357719 MS.gene009400:intron 40.0%
!! GAGAGTGCTGCAATAATCTT+CGG + chr3.4:3357876-3357895 None:intergenic 40.0%
!! GCAAGTGTCTTTGAAACTTC+TGG - chr3.4:3357646-3357665 MS.gene009400:CDS 40.0%
!! TATATTGTATGCTCTAGGGG+GGG - chr3.4:3358530-3358549 MS.gene009400:intron 40.0%
!! TTTCCACTTGGTGGAGTTTT+AGG - chr3.4:3358588-3358607 MS.gene009400:intron 40.0%
!!! GGAGTTTTAGGTAGGTTACA+AGG - chr3.4:3358600-3358619 MS.gene009400:intron 40.0%
ACTGTGCTAGCGAAACTGAA+CGG + chr3.4:3358024-3358043 None:intergenic 45.0%
AGTGATGAAGATTGGAGTGG+TGG - chr3.4:3358804-3358823 MS.gene009400:intron 45.0%
CTACCTAAAACTCCACCAAG+TGG + chr3.4:3358594-3358613 None:intergenic 45.0%
CTCCATCGATCACACGTTAT+TGG - chr3.4:3358111-3358130 MS.gene009400:CDS 45.0%
GACGAAATTGGAGACAACCA+TGG + chr3.4:3358925-3358944 None:intergenic 45.0%
GCAATGATGGCCATGATCTT+TGG + chr3.4:3357945-3357964 None:intergenic 45.0%
GTGATGAAGATTGGAGTGGT+GGG - chr3.4:3358805-3358824 MS.gene009400:intron 45.0%
TCAAATCACCCTGAAGTTGG+TGG - chr3.4:3358488-3358507 MS.gene009400:intron 45.0%
TGATGAAGATTGGAGTGGTG+GGG - chr3.4:3358806-3358825 MS.gene009400:intron 45.0%
TTGGTTACGGATGGATGATC+TGG - chr3.4:3358891-3358910 MS.gene009400:CDS 45.0%
!! CAAATCGGTTAGAGGTGTCT+TGG + chr3.4:3358379-3358398 None:intergenic 45.0%
!!! CACTTGGTGGAGTTTTAGGT+AGG - chr3.4:3358592-3358611 MS.gene009400:intron 45.0%
AACGTGTGATCGATGGAGTG+AGG + chr3.4:3358109-3358128 None:intergenic 50.0%
GATGAAGATTGGAGTGGTGG+GGG - chr3.4:3358807-3358826 MS.gene009400:intron 50.0%
TCGCTAGCACAGTTGTGCAA+TGG - chr3.4:3358031-3358050 MS.gene009400:CDS 50.0%
!! TAGAGGTGTCTTGGAGAGCA+AGG + chr3.4:3358370-3358389 None:intergenic 50.0%
!! TGTCACTATTGGCGTCGACG+AGG - chr3.4:3358999-3359018 MS.gene009400:CDS 55.0%


Chromosome Type Strat End Strand Name
chr3.4 gene 3357623 3359207 3357623 ID=MS.gene009400
chr3.4 mRNA 3357623 3359207 3357623 ID=MS.gene009400.t1;Parent=MS.gene009400
chr3.4 exon 3358850 3359207 3358850 ID=MS.gene009400.t1.exon1;Parent=MS.gene009400.t1
chr3.4 CDS 3358850 3359207 3358850 ID=cds.MS.gene009400.t1;Parent=MS.gene009400.t1
chr3.4 exon 3358623 3358765 3358623 ID=MS.gene009400.t1.exon2;Parent=MS.gene009400.t1
chr3.4 CDS 3358623 3358765 3358623 ID=cds.MS.gene009400.t1;Parent=MS.gene009400.t1
chr3.4 exon 3357966 3358485 3357966 ID=MS.gene009400.t1.exon3;Parent=MS.gene009400.t1
chr3.4 CDS 3357966 3358485 3357966 ID=cds.MS.gene009400.t1;Parent=MS.gene009400.t1
chr3.4 exon 3357810 3357910 3357810 ID=MS.gene009400.t1.exon4;Parent=MS.gene009400.t1
chr3.4 CDS 3357810 3357910 3357810 ID=cds.MS.gene009400.t1;Parent=MS.gene009400.t1
chr3.4 exon 3357623 3357697 3357623 ID=MS.gene009400.t1.exon5;Parent=MS.gene009400.t1
chr3.4 CDS 3357623 3357697 3357623 ID=cds.MS.gene009400.t1;Parent=MS.gene009400.t1
Gene Sequence

>MS.gene009400

ATGGCTGTTTTTCAAATTGAGGAGCAAGTGTCTTTGAAACTTCTGGTAAACACAGAAACCAACAAAGTTTTATTTGCTGAAGCAGGCAAAGATTTTGTGGACATTCTATGTAGCTTCTTAACCTTACCATTAGGTACAATTGCAAGACTTGTTCAGAAAGATTCAAATATGGGACCAGTTACAATTGGTTGTCTCAACTCTCTCTATCAAAGTGTCGAAAATATTGGATTGTTGCCGATTAATAATTCATCCGAAGATTATTGCAGCACTCTCAAAATTAATATTGATGATACTGTCCCTACAAATTACTTCATGTGTTCCAAAGATCATGGCCATCATTGCCCTTATTTGAGCATAGGTACTAAAAATATTTACGGTAGGTGTGGAAATCGTTTGAACCGTTCAGTTTCGCTAGCACAGTTGTGCAATGGATTTGTTAAAGGTGCTACGAATTTTGTCATTACTGATGATCTAATTGTCTCGCCTCACTCCATCGATCACACGTTATTGGATCTGCTCAAGAATTTGGGAATGAAAAACACAAGTTCAGTAAAAGAAATGACTGTCAATTTCACTAAGGAAAAGGTTTTATATTTGCAATTCATTTTAGTTTCCTAAATCATTGTTCTTAGTTTCTTTGTACTTGCTCTTATTCGCCACTTTTGAACTTTTATTTTTCAATTCACTACTGTCTTTAATTGATTAGTACTAATTCTGTACAGGTATTGGATCTGCTTAAGTGTTCCTTGCTCTCCAAGACACCTCTAACCGATTTGTTTTTGGGAAAGAAACCATCTATTGAGCGATCAATGATGCGATCAATGATTTTCTCTAGTGATGTTGAAAACATTTTTTGTAGTGATGTTCAAATCACCCTGAAGTTGGTGGTAAGAAAATCAGATGACAATATATTGTATGCTCTAGGGGGGGATGATTTTGCAAACATGATTATAGATTTTCTAACCTTTCCACTTGGTGGAGTTTTAGGTAGGTTACAAGGGAATAATTATCTAGGTAGTATTAATGGATTGCACAAGAGCTTAACTACTTTAAGTGTAGACAAGTATTTTACTTCCAAAGAAGCAAAGAACAGGCTTCTCGAGCTACGTCTTTTGCAATATTATTGTTATTATCAGTGTGATGCTCCTGATGACAGAATTATTCATGTTGAACTTTACAAAAGTGATGAAGATTGGAGTGGTGGGGGAACTTTTTCAAAAATGGAACTAGTTACAGATGAAGGTTATGTCAAAAGATCACAAATGTATTTGGTTACGGATGGATGATCTGGTCATAGAACCATGGTTGTCTCCAATTTCGTCTTTATATTTGCATAATCGTTTCAAAACTCCTCTTGATGATTTGGAGGAAAAAGTTGTCACTATTGGCGTCGACGAGGTAAATATAAAAGAGTGAAAACTATATGTATTTATCACCTACACTATATGTATTTTTACTATGGGACTGATTATGCAGCAGTTGCTCATCTCTCATTGTTGTGTTTGTGCAGAGTTACAGCATATTAAAGGCAGCTTTAAGCTCAACATCTGCTCTCACAAATGGTCTTCGTCACTTGTTAACCTAA
Protein sequence

>MS.gene009400.t1

MAVFQIEEQVSLKLLVNTETNKVLFAEAGKDFVDILCSFLTLPLGTIARLVQKDSNMGPVTIGCLNSLYQSVENIGLLPINNSSEDYCSTLKINIDDTVPTNYFMCSKDHGHHCPYLSIGATNFVITDDLIVSPHSIDHTLLDLLKNLGMKNTSSVKEMTVNFTKEKVLDLLKCSLLSKTPLTDLFLGKKPSIERSMMRSMIFSSDVENIFCSDVQITLKLVVRKSDDNILYALGGDDFANMIIDFLTFPLGGVLGRLQGNNYLGSINGLHKSLTTLSVDKYFTSKEAKNRLLELRLLQYYCYYQCDAPDDRIIHVELYKSDEDWSGGGTFSKMELVTDEEPWLSPISSLYLHNRFKTPLDDLEEKVVTIGVDESYSILKAALSSTSALTNGLRHLLT