AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055035

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055035.t1 MTR_7g066570 78.163 577 47 4 1 505 1 570 0.0 865
MS.gene055035.t1 MTR_7g012680 64.228 492 97 4 1 455 1 450 0.0 584
MS.gene055035.t1 MTR_7g066260 54.111 523 160 9 6 484 7 493 1.12e-166 481
MS.gene055035.t1 MTR_7g058710 52.087 503 170 8 1 454 1 481 1.83e-164 474
MS.gene055035.t1 MTR_7g066340 53.280 503 157 8 5 466 3 468 4.30e-159 461
MS.gene055035.t1 MTR_7g066350 55.647 487 135 10 10 451 5 455 4.85e-157 455
MS.gene055035.t1 MTR_7g066350 31.250 240 131 10 224 444 3 227 2.26e-14 75.9
MS.gene055035.t1 MTR_7g066580 55.088 452 175 8 20 452 1 443 2.63e-152 442
MS.gene055035.t1 MTR_1g041315 51.959 485 148 7 1 434 1 451 1.15e-151 442
MS.gene055035.t1 MTR_7g066400 49.254 469 165 9 10 447 8 434 3.82e-137 404
MS.gene055035.t1 MTR_2g029400 52.135 445 168 8 1 440 1 405 3.19e-136 400
MS.gene055035.t1 MTR_7g066290 50.102 491 143 9 2 450 3 433 3.83e-135 398
MS.gene055035.t1 MTR_7g066360 48.119 505 179 8 1 464 1 463 2.22e-132 392
MS.gene055035.t1 MTR_7g066310 48.414 473 164 8 11 473 4 406 3.10e-127 377
MS.gene055035.t1 MTR_7g066310 31.282 195 119 6 10 195 189 377 4.05e-11 65.5
MS.gene055035.t1 MTR_7g066430 42.394 493 228 6 15 457 5 491 4.62e-121 364
MS.gene055035.t1 MTR_7g058700 43.595 484 129 8 26 454 531 925 8.79e-112 353
MS.gene055035.t1 MTR_7g066410 39.726 511 226 11 14 450 10 512 1.04e-108 333
MS.gene055035.t1 MTR_7g063920 40.821 463 236 9 1 454 1 434 3.46e-104 319
MS.gene055035.t1 MTR_7g063820 40.486 494 236 9 6 454 2 482 5.58e-103 317
MS.gene055035.t1 MTR_7g062280 37.398 492 247 7 13 454 9 489 1.54e-102 316
MS.gene055035.t1 MTR_7g062280 30.837 227 111 5 9 195 259 479 1.55e-16 82.4
MS.gene055035.t1 MTR_7g062300 38.462 494 235 12 1 448 1 471 6.94e-102 314
MS.gene055035.t1 MTR_7g063880 40.283 494 227 13 1 454 1 466 2.75e-100 310
MS.gene055035.t1 MTR_7g063660 39.300 486 233 11 13 454 8 475 1.24e-99 308
MS.gene055035.t1 MTR_7g063900 41.068 487 229 12 15 454 11 486 4.02e-99 307
MS.gene055035.t1 MTR_7g063840 37.372 487 256 9 1 454 1 471 3.15e-96 300
MS.gene055035.t1 MTR_7g063890 41.420 507 228 12 2 454 11 502 6.95e-96 300
MS.gene055035.t1 MTR_3g007740 37.732 485 224 9 1 450 1 442 6.69e-88 277
MS.gene055035.t1 MTR_7g066680 50.000 304 111 3 179 447 38 335 1.25e-84 265
MS.gene055035.t1 MTR_7g063830 36.439 483 223 10 6 450 2 438 7.02e-83 264
MS.gene055035.t1 MTR_7g063670 35.671 499 229 13 1 454 4 455 2.22e-82 263
MS.gene055035.t1 MTR_7g066280 39.359 437 185 9 93 473 1 413 1.23e-80 257
MS.gene055035.t1 MTR_3g065200 36.580 462 195 11 15 436 4 407 2.51e-80 258
MS.gene055035.t1 MTR_4g019310 34.686 493 242 12 17 446 11 486 6.24e-77 250
MS.gene055035.t1 MTR_7g066480 44.164 317 143 4 15 297 17 333 3.93e-74 241
MS.gene055035.t1 MTR_7g066480 54.878 82 37 0 371 452 342 423 2.17e-18 87.8
MS.gene055035.t1 MTR_7g066480 28.022 182 100 5 15 195 264 415 1.39e-12 70.1
MS.gene055035.t1 MTR_4g017830 34.893 513 249 15 6 449 4 500 2.65e-73 241
MS.gene055035.t1 MTR_4g017440 33.918 513 275 13 1 455 1 507 3.18e-73 241
MS.gene055035.t1 MTR_4g017610 34.560 489 258 13 5 447 4 476 2.82e-71 234
MS.gene055035.t1 MTR_4g017470 33.665 502 288 12 1 470 1 489 8.88e-70 231
MS.gene055035.t1 MTR_4g019340 35.551 481 235 11 15 438 39 501 2.82e-68 228
MS.gene055035.t1 MTR_1g014060 51.754 228 71 3 23 213 9 234 1.03e-66 220
MS.gene055035.t1 MTR_1g014060 53.846 208 74 4 249 455 203 389 3.58e-55 190
MS.gene055035.t1 MTR_1g014060 27.885 208 130 8 239 436 12 209 1.34e-11 66.6
MS.gene055035.t1 MTR_4g017410 32.008 528 270 17 8 478 3 498 3.02e-65 219
MS.gene055035.t1 MTR_7g066490 43.082 318 131 5 15 297 17 319 3.15e-65 217
MS.gene055035.t1 MTR_7g066490 47.706 109 56 1 344 452 302 409 1.49e-18 88.2
MS.gene055035.t1 MTR_7g066490 27.778 180 99 5 17 195 252 401 1.22e-11 67.0
MS.gene055035.t1 MTR_4g017550 31.878 458 254 13 41 446 18 469 3.36e-51 181
MS.gene055035.t1 MTR_7g063720 38.655 238 122 4 219 454 15 230 1.30e-44 157
MS.gene055035.t1 MTR_7g063720 26.941 219 128 6 17 211 26 236 8.24e-16 77.4
MS.gene055035.t1 MTR_7g062270 43.407 182 64 3 1 147 1 178 1.22e-36 140
MS.gene055035.t1 MTR_2g067310 54.225 142 51 3 25 161 58 190 5.10e-36 134
MS.gene055035.t1 MTR_7g063850 31.613 310 179 8 151 454 4 286 5.02e-34 130
MS.gene055035.t1 MTR_8g023240 26.261 476 275 16 15 444 38 483 8.00e-31 126
MS.gene055035.t1 MTR_7g063690 33.846 260 128 9 1 254 1 222 2.97e-28 113
MS.gene055035.t1 MTR_7g100420 32.727 275 172 5 178 450 134 397 1.91e-25 108
MS.gene055035.t1 MTR_7g100420 55.844 77 28 2 15 85 40 116 7.12e-14 73.9
MS.gene055035.t1 MTR_8g445860 29.921 254 136 9 207 442 1 230 5.88e-25 103
MS.gene055035.t1 MTR_8g445860 29.694 229 113 6 15 200 14 237 2.11e-19 87.8
MS.gene055035.t1 MTR_7g066390 54.082 98 44 1 362 459 35 131 6.53e-24 98.2
MS.gene055035.t1 MTR_1g115440 29.675 246 119 5 1 200 1 238 1.16e-18 85.9
MS.gene055035.t1 MTR_0150s0010 27.386 241 124 5 6 200 2 237 7.84e-18 83.2
MS.gene055035.t1 MTR_4g019240 39.130 115 60 3 10 116 9 121 6.25e-17 79.3
MS.gene055035.t1 MTR_7g063760 49.296 71 35 1 385 454 1 71 6.40e-17 75.9
MS.gene055035.t1 MTR_7g063870 31.214 173 72 4 8 179 25 151 3.81e-16 78.6
MS.gene055035.t1 MTR_1g115455 27.632 228 118 4 15 200 10 232 1.78e-15 76.3
MS.gene055035.t1 MTR_1g115450 39.286 84 46 1 15 98 8 86 2.02e-14 70.9
MS.gene055035.t1 MTR_1g115435 29.187 209 117 9 17 202 10 210 2.26e-13 69.7
MS.gene055035.t1 MTR_1g115470 39.362 94 50 2 17 108 9 97 1.29e-12 70.1
MS.gene055035.t1 MTR_8g445920 35.165 91 54 1 6 96 2 87 4.69e-11 60.1
MS.gene055035.t1 MTR_8g044890 35.165 91 54 1 6 96 2 87 4.69e-11 60.1
MS.gene055035.t1 MTR_0150s0030 37.805 82 45 2 17 97 11 87 7.53e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055035.t1 AT5G01120 31.600 500 277 10 15 461 10 497 1.09e-64 218
MS.gene055035.t1 AT5G43240 30.857 525 266 11 15 467 10 509 2.82e-61 209
MS.gene055035.t1 AT5G43240 30.857 525 266 11 15 467 10 509 2.82e-61 209
MS.gene055035.t1 AT5G43240 30.857 525 266 11 15 467 10 509 2.82e-61 209
MS.gene055035.t1 AT3G09140 31.992 497 260 10 17 450 12 493 1.18e-59 205
MS.gene055035.t1 AT5G01140 30.947 475 252 8 17 450 12 451 6.17e-56 194
MS.gene055035.t1 AT5G01140 26.818 220 121 7 15 200 241 454 5.90e-12 68.2
MS.gene055035.t1 AT5G01130 32.505 483 248 12 6 450 2 444 3.62e-54 189
MS.gene055035.t1 AT3G09140 30.286 525 260 11 17 450 12 521 9.69e-54 189
MS.gene055035.t1 AT5G01150 31.733 479 271 9 17 450 12 479 3.24e-53 187
MS.gene055035.t1 AT5G01150 24.723 271 166 8 222 467 4 261 2.71e-13 72.4
MS.gene055035.t1 AT3G09110 34.628 309 165 3 17 289 12 319 1.91e-52 181
MS.gene055035.t1 AT5G37320 27.364 497 255 13 24 450 19 479 3.52e-40 151
MS.gene055035.t1 AT3G09120 31.731 312 167 4 13 281 5 313 9.30e-40 146
MS.gene055035.t1 AT3G09120 27.468 233 143 8 226 442 5 227 4.37e-11 64.7
MS.gene055035.t1 AT5G43240 30.220 364 203 10 130 467 27 365 2.13e-29 119

Find 88 sgRNAs with CRISPR-Local

Find 145 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTAACAGTTCTTATAAAT+TGG 0.162711 7.4:-37605707 None:intergenic
GCTTCTTAACATTGCCTTTA+GGG 0.201640 7.4:+37604212 MS.gene055035:CDS
TCCTCCACCAAAGTAGATTT+TGG 0.204635 7.4:-37605888 None:intergenic
GAGGAAAGAGTTATCAAAAT+TGG 0.208695 7.4:+37605543 MS.gene055035:CDS
AATATTGAAGAAGTGAAATT+TGG 0.233062 7.4:+37604265 MS.gene055035:CDS
TTGGGATCAACCAATTTAAA+TGG 0.251681 7.4:-37605384 None:intergenic
TGGTTCTTGAGTTCAAATTC+TGG 0.265338 7.4:-37605222 None:intergenic
GCTCTTTCGTAAAATAAAAT+TGG 0.265550 7.4:-37604875 None:intergenic
AGTCCCTCTACATGATTTAG+AGG 0.308031 7.4:+37605524 MS.gene055035:CDS
AGCTTCTTAACATTGCCTTT+AGG 0.310196 7.4:+37604211 MS.gene055035:CDS
CAGCCTACTTAAAGCTTCAT+TGG 0.321652 7.4:+37605653 MS.gene055035:CDS
TAATCTTCCATGGAGTTTCT+TGG 0.323499 7.4:-37604373 None:intergenic
GGTTGATCCCAAGTCTTCTA+TGG 0.337630 7.4:+37605395 MS.gene055035:CDS
CAAAGGACCATCAATGTATA+TGG 0.337667 7.4:+37605434 MS.gene055035:CDS
AGTACTTCGTGTGCGAGAAT+TGG 0.338229 7.4:+37604440 MS.gene055035:CDS
TTCAACTGATTCACTTTGAA+TGG 0.341437 7.4:-37604092 None:intergenic
TTTGGTTTAGCCCCATCTTC+AGG 0.343943 7.4:-37605870 None:intergenic
TTTGAAACTAAGAAACAATA+TGG 0.354003 7.4:+37604912 MS.gene055035:CDS
CAGCACCAATTGGTAATATC+TGG 0.361156 7.4:-37605242 None:intergenic
AACAATATGGAGTTTCTGAT+TGG 0.370003 7.4:+37604925 MS.gene055035:CDS
CATATACATTGATGGTCCTT+TGG 0.380788 7.4:-37605433 None:intergenic
TAGAAATGACATAGTAGAAA+TGG 0.386822 7.4:-37605490 None:intergenic
CCAATTTATAAGAACTGTTA+AGG 0.387413 7.4:+37605707 MS.gene055035:CDS
TCAACTTCTGCTCCTGAAGA+TGG 0.397724 7.4:+37605858 MS.gene055035:CDS
GTAAAATAAAATTGGTCAAA+GGG 0.398241 7.4:-37604867 None:intergenic
TTAAATCTACTTCAAAAGCT+TGG 0.402949 7.4:+37604652 MS.gene055035:CDS
TCCTGACCTTACAAAAGAAA+TGG 0.404064 7.4:+37605320 MS.gene055035:CDS
TAATGTTCGTCTGAGAGTTT+TGG 0.408151 7.4:+37604123 MS.gene055035:CDS
GGTTCTTGAGTTCAAATTCT+GGG 0.408472 7.4:-37605221 None:intergenic
GTACTTCGTGTGCGAGAATT+GGG 0.414321 7.4:+37604441 MS.gene055035:CDS
TAGTAATACCTGTATGTTAC+GGG 0.419068 7.4:-37605922 None:intergenic
CAACTTCTGCTCCTGAAGAT+GGG 0.421746 7.4:+37605859 MS.gene055035:CDS
CAAATTCTGTTTGTCGAAGC+AGG 0.430694 7.4:+37605012 MS.gene055035:CDS
GTTTCAAATTATCAACAGAT+TGG 0.441849 7.4:-37604897 None:intergenic
TCTGTCACCATATACATTGA+TGG 0.453671 7.4:-37605441 None:intergenic
CGCAAGAAAGCAGCACCAAT+TGG 0.454787 7.4:-37605252 None:intergenic
CATATGCAACACTCCTCCTA+AGG 0.479612 7.4:-37605076 None:intergenic
GGCTAAACCAAAATCTACTT+TGG 0.484146 7.4:+37605881 MS.gene055035:CDS
CGTAAAATAAAATTGGTCAA+AGG 0.486886 7.4:-37604868 None:intergenic
GGAGTGTTGCATATGCTTCA+AGG 0.493604 7.4:+37605084 MS.gene055035:CDS
CGAGACTATTGATGCTATTG+AGG 0.494678 7.4:+37604678 MS.gene055035:CDS
ATTCCAATGAAGCTTTAAGT+AGG 0.497354 7.4:-37605656 None:intergenic
ATAGTAATACCTGTATGTTA+CGG 0.499348 7.4:-37605923 None:intergenic
AAAGTTGTGTATGCTGAGGC+TGG 0.508934 7.4:+37604163 MS.gene055035:CDS
TTTCTAACCTTCCCCTTAGG+AGG 0.508980 7.4:+37605063 MS.gene055035:CDS
TCAGACATTAAATATATGTC+CGG 0.511445 7.4:-37605162 None:intergenic
GATCTTTGCAAAGCCAATGC+AGG 0.515857 7.4:-37606559 None:intergenic
ACCAAAATCTACTTTGGTGG+AGG 0.521894 7.4:+37605887 MS.gene055035:CDS
AAGAACCAGATATTACCAAT+TGG 0.527915 7.4:+37605237 MS.gene055035:CDS
TGTTGACATCAACAAGAAAG+AGG 0.533984 7.4:+37604708 MS.gene055035:CDS
GCGCCTGCCCCGTAACATAC+AGG 0.534922 7.4:+37605914 MS.gene055035:CDS
CTATATTTAGTTTCAGCTGT+CGG 0.539959 7.4:-37604399 None:intergenic
GGACATATATTTAATGTCTG+AGG 0.545390 7.4:+37605164 MS.gene055035:CDS
CTGTCGGCAGTAATCTTCCA+TGG 0.545539 7.4:-37604383 None:intergenic
ATATGCAACACTCCTCCTAA+GGG 0.551461 7.4:-37605075 None:intergenic
GAATTGGGATTGTAGAAGAA+AGG 0.552800 7.4:+37604456 MS.gene055035:CDS
AGTTATCAAAATTGGCGTCA+AGG 0.564487 7.4:+37605551 MS.gene055035:CDS
GACATATATTTAATGTCTGA+GGG 0.566806 7.4:+37605165 MS.gene055035:CDS
AGAGAACAAGACTCCTGCAT+TGG 0.566936 7.4:+37606546 MS.gene055035:CDS
TATTGCAAGACTCGTTGCTA+AGG 0.567646 7.4:+37604237 MS.gene055035:CDS
TAAACCAAAATCTACTTTGG+TGG 0.568709 7.4:+37605884 MS.gene055035:CDS
AGTCTTGCAATAGTCCCTAA+AGG 0.571615 7.4:-37604226 None:intergenic
AACTTCTGCTCCTGAAGATG+GGG 0.581248 7.4:+37605860 MS.gene055035:CDS
CTTTCCTCTAAATCATGTAG+AGG 0.584914 7.4:-37605528 None:intergenic
GTTCTTGAGTTCAAATTCTG+GGG 0.586259 7.4:-37605220 None:intergenic
TGGCTTTGCAAAGATCAACT+AGG 0.586300 7.4:+37606566 MS.gene055035:CDS
TCAAATTCTGGGGTAATGAG+AGG 0.595346 7.4:-37605210 None:intergenic
CAACACTCCTCCTAAGGGGA+AGG 0.595804 7.4:-37605070 None:intergenic
ATACCTGTATGTTACGGGGC+AGG 0.595942 7.4:-37605917 None:intergenic
CTCGCACACGAAGTACTGCA+AGG 0.596798 7.4:-37604434 None:intergenic
TAAAATAAAATTGGTCAAAG+GGG 0.605375 7.4:-37604866 None:intergenic
ATAGTAGAAATGGACGACAT+TGG 0.606118 7.4:-37605480 None:intergenic
GTCTATCCTCATCAGATGGT+AGG 0.607538 7.4:-37604951 None:intergenic
ACTGCAACCAAGAAACTCCA+TGG 0.607950 7.4:+37604366 MS.gene055035:CDS
TTTGTGCTCACGTACATGCA+AGG 0.610161 7.4:+37604336 MS.gene055035:CDS
GCAAAATCATCATATGCCAA+AGG 0.610317 7.4:+37605417 MS.gene055035:CDS
TGTGTATGCTGAGGCTGGGA+AGG 0.614514 7.4:+37604168 MS.gene055035:CDS
ATTTGTCTATCCTCATCAGA+TGG 0.623409 7.4:-37604955 None:intergenic
TTTCCTCTAAATCATGTAGA+GGG 0.629418 7.4:-37605527 None:intergenic
TGGAGACCTACCATCTGATG+AGG 0.632344 7.4:+37604945 MS.gene055035:CDS
ACTATGATTACACAAGGCAG+AGG 0.642345 7.4:+37605295 MS.gene055035:CDS
AAGTTGTGTATGCTGAGGCT+GGG 0.646725 7.4:+37604164 MS.gene055035:CDS
TCGCACACGAAGTACTGCAA+GGG 0.658925 7.4:-37604433 None:intergenic
TATGCAACACTCCTCCTAAG+GGG 0.688986 7.4:-37605074 None:intergenic
TATCAAAATTGGCGTCAAGG+AGG 0.703000 7.4:+37605554 MS.gene055035:CDS
AGTAATACCTGTATGTTACG+GGG 0.732614 7.4:-37605921 None:intergenic
ATACTTACTATGATTACACA+AGG 0.733669 7.4:+37605289 MS.gene055035:CDS
GAACAAAGTTGTGTATGCTG+AGG 0.744300 7.4:+37604159 MS.gene055035:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAATTTGATTAACAATAT+AGG + chr7.4:37606391-37606410 MS.gene055035:intron 10.0%
!! AAAGAAATGAAAAATTGTTT+AGG - chr7.4:37606091-37606110 None:intergenic 15.0%
!!! AAGTTTTTCAATATAGAAAA+TGG + chr7.4:37604556-37604575 MS.gene055035:intron 15.0%
!!! TTATTATGTTTTTTGAGATT+AGG + chr7.4:37606129-37606148 MS.gene055035:intron 15.0%
!!! TTTTCTATATTGAAAAACTT+CGG - chr7.4:37604556-37604575 None:intergenic 15.0%
!! AAAAAATGCACCATTTAAAT+TGG + chr7.4:37605374-37605393 MS.gene055035:CDS 20.0%
!! AATATTGAAGAAGTGAAATT+TGG + chr7.4:37604265-37604284 MS.gene055035:CDS 20.0%
!! ACTTGATAAGGAAAATATTT+AGG - chr7.4:37605743-37605762 None:intergenic 20.0%
!! GTAAAATAAAATTGGTCAAA+GGG - chr7.4:37604870-37604889 None:intergenic 20.0%
!! TAAAATAAAATTGGTCAAAG+GGG - chr7.4:37604869-37604888 None:intergenic 20.0%
!! TTTCTATATTGAAAAACTTC+GGG - chr7.4:37604555-37604574 None:intergenic 20.0%
!!! AATAAACCATTTCTTTTGTA+AGG - chr7.4:37605329-37605348 None:intergenic 20.0%
!!! ACAATTTTTCATTTCTTTCA+TGG + chr7.4:37606093-37606112 MS.gene055035:intron 20.0%
!!! ATTAACAATATAGGTAGTTT+TGG + chr7.4:37606400-37606419 MS.gene055035:intron 20.0%
!!! ATTTCTTATTCGATTTGTAT+AGG + chr7.4:37604807-37604826 MS.gene055035:intron 20.0%
!!! TTTGAAACTAAGAAACAATA+TGG + chr7.4:37604912-37604931 MS.gene055035:CDS 20.0%
! AGAGTAATCTAAACTTGATA+AGG - chr7.4:37605755-37605774 None:intergenic 25.0%
! ATACTTACTATGATTACACA+AGG + chr7.4:37605289-37605308 MS.gene055035:CDS 25.0%
! ATAGTAATACCTGTATGTTA+CGG - chr7.4:37605926-37605945 None:intergenic 25.0%
! ATATATAACGTGTGAAGTAA+AGG - chr7.4:37606238-37606257 None:intergenic 25.0%
! ATTTATAAGAACTGTTAAGG+TGG + chr7.4:37605710-37605729 MS.gene055035:intron 25.0%
! CATACAGGTATTACTATTTA+TGG + chr7.4:37605929-37605948 MS.gene055035:intron 25.0%
! CCAATTTATAAGAACTGTTA+AGG + chr7.4:37605707-37605726 MS.gene055035:CDS 25.0%
! CCTTAACAGTTCTTATAAAT+TGG - chr7.4:37605710-37605729 None:intergenic 25.0%
! CGTAAAATAAAATTGGTCAA+AGG - chr7.4:37604871-37604890 None:intergenic 25.0%
! GACATATATTTAATGTCTGA+GGG + chr7.4:37605165-37605184 MS.gene055035:CDS 25.0%
! GCTCTTTCGTAAAATAAAAT+TGG - chr7.4:37604878-37604897 None:intergenic 25.0%
! GTTTCAAATTATCAACAGAT+TGG - chr7.4:37604900-37604919 None:intergenic 25.0%
! TAGAAATGACATAGTAGAAA+TGG - chr7.4:37605493-37605512 None:intergenic 25.0%
! TATCATCAACAAAAGTAAGT+TGG - chr7.4:37605824-37605843 None:intergenic 25.0%
! TCAGACATTAAATATATGTC+CGG - chr7.4:37605165-37605184 None:intergenic 25.0%
! TTAAATCTACTTCAAAAGCT+TGG + chr7.4:37604652-37604671 MS.gene055035:CDS 25.0%
!! TGTCAGTATCTATATCTTTT+TGG + chr7.4:37606485-37606504 MS.gene055035:intron 25.0%
!!! TATAGAAAATGGTTTTGTGA+AGG + chr7.4:37604567-37604586 MS.gene055035:intron 25.0%
!!! TATCTATATCTTTTTGGAAG+AGG + chr7.4:37606491-37606510 MS.gene055035:intron 25.0%
!!! TTTTTTGCAATATTGATGCA+TGG - chr7.4:37606006-37606025 None:intergenic 25.0%
AACAATATGGAGTTTCTGAT+TGG + chr7.4:37604925-37604944 MS.gene055035:CDS 30.0%
AAGAACCAGATATTACCAAT+TGG + chr7.4:37605237-37605256 MS.gene055035:CDS 30.0%
AGGTTAGTATATCTTTAGCA+CGG - chr7.4:37605799-37605818 None:intergenic 30.0%
ATACATAACAATAGCAACAC+TGG - chr7.4:37606451-37606470 None:intergenic 30.0%
ATTACTCTCTTCTCCATAAT+AGG + chr7.4:37605767-37605786 MS.gene055035:intron 30.0%
GAGGAAAGAGTTATCAAAAT+TGG + chr7.4:37605543-37605562 MS.gene055035:CDS 30.0%
GGACATATATTTAATGTCTG+AGG + chr7.4:37605164-37605183 MS.gene055035:CDS 30.0%
TAAACCAAAATCTACTTTGG+TGG + chr7.4:37605884-37605903 MS.gene055035:CDS 30.0%
TAGTAATACCTGTATGTTAC+GGG - chr7.4:37605925-37605944 None:intergenic 30.0%
TTACTCTCTTCTCCATAATA+GGG + chr7.4:37605768-37605787 MS.gene055035:intron 30.0%
TTGGGATCAACCAATTTAAA+TGG - chr7.4:37605387-37605406 None:intergenic 30.0%
TTTCCTCTAAATCATGTAGA+GGG - chr7.4:37605530-37605549 None:intergenic 30.0%
! AAACTTGCTCCAAAAACATT+AGG - chr7.4:37604634-37604653 None:intergenic 30.0%
! AACACTGGTATTTTCAATTC+AGG + chr7.4:37606519-37606538 MS.gene055035:intron 30.0%
! ACTTTTTGTTACATGTCACA+GGG + chr7.4:37605627-37605646 MS.gene055035:intron 30.0%
! CTATATTTAGTTTCAGCTGT+CGG - chr7.4:37604402-37604421 None:intergenic 30.0%
! TGTGTTTTGCCTAATGTTTT+TGG + chr7.4:37604622-37604641 MS.gene055035:CDS 30.0%
! TTCAACTGATTCACTTTGAA+TGG - chr7.4:37604095-37604114 None:intergenic 30.0%
!! ATTCCAATGAAGCTTTAAGT+AGG - chr7.4:37605659-37605678 None:intergenic 30.0%
!! TACTTTTTGTTACATGTCAC+AGG + chr7.4:37605626-37605645 MS.gene055035:intron 30.0%
!! TCAACAAAAGTAAGTTGGTT+AGG - chr7.4:37605819-37605838 None:intergenic 30.0%
!! TCAACATCTGCTTTGAAAAA+TGG + chr7.4:37605678-37605697 MS.gene055035:CDS 30.0%
AGCTTCTTAACATTGCCTTT+AGG + chr7.4:37604211-37604230 MS.gene055035:CDS 35.0%
AGGAACCAAAAATGCTATTG+TGG + chr7.4:37604505-37604524 MS.gene055035:intron 35.0%
AGGAAGCAAAAAGTCAACAA+GGG - chr7.4:37606071-37606090 None:intergenic 35.0%
AGTAATACCTGTATGTTACG+GGG - chr7.4:37605924-37605943 None:intergenic 35.0%
AGTTATCAAAATTGGCGTCA+AGG + chr7.4:37605551-37605570 MS.gene055035:CDS 35.0%
ATAGTAGAAATGGACGACAT+TGG - chr7.4:37605483-37605502 None:intergenic 35.0%
ATTTGTCTATCCTCATCAGA+TGG - chr7.4:37604958-37604977 None:intergenic 35.0%
CAAAGGACCATCAATGTATA+TGG + chr7.4:37605434-37605453 MS.gene055035:CDS 35.0%
CTTTCCTCTAAATCATGTAG+AGG - chr7.4:37605531-37605550 None:intergenic 35.0%
GAATTGGGATTGTAGAAGAA+AGG + chr7.4:37604456-37604475 MS.gene055035:CDS 35.0%
GATTGTAGAAGAAAGGTAAG+TGG + chr7.4:37604463-37604482 MS.gene055035:intron 35.0%
GCAAAATCATCATATGCCAA+AGG + chr7.4:37605417-37605436 MS.gene055035:CDS 35.0%
GCTTCTTAACATTGCCTTTA+GGG + chr7.4:37604212-37604231 MS.gene055035:CDS 35.0%
GGAACCAAAAATGCTATTGT+GGG + chr7.4:37604506-37604525 MS.gene055035:intron 35.0%
GGCTAAACCAAAATCTACTT+TGG + chr7.4:37605881-37605900 MS.gene055035:CDS 35.0%
GGTTCCTAAAAAAGCTCAAA+AGG - chr7.4:37604492-37604511 None:intergenic 35.0%
GGTTCTTGAGTTCAAATTCT+GGG - chr7.4:37605224-37605243 None:intergenic 35.0%
GTTCTTGAGTTCAAATTCTG+GGG - chr7.4:37605223-37605242 None:intergenic 35.0%
TAACCGAATCCTCTATTTGA+TGG + chr7.4:37605978-37605997 MS.gene055035:intron 35.0%
TAATCTTCCATGGAGTTTCT+TGG - chr7.4:37604376-37604395 None:intergenic 35.0%
TAGGAAGCAAAAAGTCAACA+AGG - chr7.4:37606072-37606091 None:intergenic 35.0%
TCAACAAGGGTTTGACATAT+TGG - chr7.4:37606058-37606077 None:intergenic 35.0%
TCCTGACCTTACAAAAGAAA+TGG + chr7.4:37605320-37605339 MS.gene055035:CDS 35.0%
TCTGTCACCATATACATTGA+TGG - chr7.4:37605444-37605463 None:intergenic 35.0%
TGAGACATCATTTGAACTGT+CGG - chr7.4:37606208-37606227 None:intergenic 35.0%
TGGTTCTTGAGTTCAAATTC+TGG - chr7.4:37605225-37605244 None:intergenic 35.0%
TGTTGACATCAACAAGAAAG+AGG + chr7.4:37604708-37604727 MS.gene055035:CDS 35.0%
! ACCATTTCTTTTGTAAGGTC+AGG - chr7.4:37605324-37605343 None:intergenic 35.0%
! CATATACATTGATGGTCCTT+TGG - chr7.4:37605436-37605455 None:intergenic 35.0%
! CTTTCCCACAATAGCATTTT+TGG - chr7.4:37604513-37604532 None:intergenic 35.0%
! GATTTTGCCATAGAAGACTT+GGG - chr7.4:37605405-37605424 None:intergenic 35.0%
! TAATGTTCGTCTGAGAGTTT+TGG + chr7.4:37604123-37604142 MS.gene055035:CDS 35.0%
! TGATTTTGCCATAGAAGACT+TGG - chr7.4:37605406-37605425 None:intergenic 35.0%
! TTTTCGTCTTTAAGCAGCAT+TGG + chr7.4:37605108-37605127 MS.gene055035:CDS 35.0%
!! GATGCCTTTTGAGCTTTTTT+AGG + chr7.4:37604485-37604504 MS.gene055035:intron 35.0%
!! TGTTGATGATATGAACATGC+AGG + chr7.4:37605833-37605852 MS.gene055035:intron 35.0%
!!! AGAAAGAGGCATGCTTTTTT+CGG + chr7.4:37604722-37604741 MS.gene055035:intron 35.0%
ACCAAAATCTACTTTGGTGG+AGG + chr7.4:37605887-37605906 MS.gene055035:CDS 40.0%
ACTATGATTACACAAGGCAG+AGG + chr7.4:37605295-37605314 MS.gene055035:CDS 40.0%
AGCACGGAATAACCCTATTA+TGG - chr7.4:37605783-37605802 None:intergenic 40.0%
AGTCCCTCTACATGATTTAG+AGG + chr7.4:37605524-37605543 MS.gene055035:CDS 40.0%
AGTCTTGCAATAGTCCCTAA+AGG - chr7.4:37604229-37604248 None:intergenic 40.0%
ATATGCAACACTCCTCCTAA+GGG - chr7.4:37605078-37605097 None:intergenic 40.0%
CAAATTCTGTTTGTCGAAGC+AGG + chr7.4:37605012-37605031 MS.gene055035:CDS 40.0%
CAGCCTACTTAAAGCTTCAT+TGG + chr7.4:37605653-37605672 MS.gene055035:CDS 40.0%
CGAGACTATTGATGCTATTG+AGG + chr7.4:37604678-37604697 MS.gene055035:CDS 40.0%
GAACAAAGTTGTGTATGCTG+AGG + chr7.4:37604159-37604178 MS.gene055035:CDS 40.0%
GCAACGATGAACACTAACAA+AGG + chr7.4:37606344-37606363 MS.gene055035:intron 40.0%
GCTCCATCAAATAGAGGATT+CGG - chr7.4:37605984-37606003 None:intergenic 40.0%
TATCAAAATTGGCGTCAAGG+AGG + chr7.4:37605554-37605573 MS.gene055035:CDS 40.0%
TATTGCAAGACTCGTTGCTA+AGG + chr7.4:37604237-37604256 MS.gene055035:CDS 40.0%
! AGTTTTCTAACCTTCCCCTT+AGG + chr7.4:37605060-37605079 MS.gene055035:CDS 40.0%
! CAGCACCAATTGGTAATATC+TGG - chr7.4:37605245-37605264 None:intergenic 40.0%
! TCCTCCACCAAAGTAGATTT+TGG - chr7.4:37605891-37605910 None:intergenic 40.0%
! TGGCTTTGCAAAGATCAACT+AGG + chr7.4:37606566-37606585 MS.gene055035:CDS 40.0%
!! GAGCTTTTCTGATTTGAGTC+CGG + chr7.4:37605143-37605162 MS.gene055035:CDS 40.0%
!! TCAAATTCTGGGGTAATGAG+AGG - chr7.4:37605213-37605232 None:intergenic 40.0%
AAAGTTGTGTATGCTGAGGC+TGG + chr7.4:37604163-37604182 MS.gene055035:CDS 45.0%
AACTTCTGCTCCTGAAGATG+GGG + chr7.4:37605860-37605879 MS.gene055035:CDS 45.0%
AAGTTGTGTATGCTGAGGCT+GGG + chr7.4:37604164-37604183 MS.gene055035:CDS 45.0%
ACTGCAACCAAGAAACTCCA+TGG + chr7.4:37604366-37604385 MS.gene055035:CDS 45.0%
AGAGAACAAGACTCCTGCAT+TGG + chr7.4:37606546-37606565 MS.gene055035:CDS 45.0%
AGTACTTCGTGTGCGAGAAT+TGG + chr7.4:37604440-37604459 MS.gene055035:CDS 45.0%
CAACTTCTGCTCCTGAAGAT+GGG + chr7.4:37605859-37605878 MS.gene055035:CDS 45.0%
CATATGCAACACTCCTCCTA+AGG - chr7.4:37605079-37605098 None:intergenic 45.0%
GATCTTTGCAAAGCCAATGC+AGG - chr7.4:37606562-37606581 None:intergenic 45.0%
GGTTGATCCCAAGTCTTCTA+TGG + chr7.4:37605395-37605414 MS.gene055035:CDS 45.0%
GTACTTCGTGTGCGAGAATT+GGG + chr7.4:37604441-37604460 MS.gene055035:CDS 45.0%
GTCTATCCTCATCAGATGGT+AGG - chr7.4:37604954-37604973 None:intergenic 45.0%
TATGCAACACTCCTCCTAAG+GGG - chr7.4:37605077-37605096 None:intergenic 45.0%
TCAACTTCTGCTCCTGAAGA+TGG + chr7.4:37605858-37605877 MS.gene055035:CDS 45.0%
TGCATGGCTCCATCAAATAG+AGG - chr7.4:37605990-37606009 None:intergenic 45.0%
TTGGAAGAGGAAGCTAACAC+TGG + chr7.4:37606504-37606523 MS.gene055035:intron 45.0%
TTTCTAACCTTCCCCTTAGG+AGG + chr7.4:37605063-37605082 MS.gene055035:CDS 45.0%
TTTGTGCTCACGTACATGCA+AGG + chr7.4:37604336-37604355 MS.gene055035:CDS 45.0%
! TTTGGTTTAGCCCCATCTTC+AGG - chr7.4:37605873-37605892 None:intergenic 45.0%
!! GGAGTGTTGCATATGCTTCA+AGG + chr7.4:37605084-37605103 MS.gene055035:CDS 45.0%
!! TGTTCGTCTGAGAGTTTTGG+TGG + chr7.4:37604126-37604145 MS.gene055035:CDS 45.0%
ATACCTGTATGTTACGGGGC+AGG - chr7.4:37605920-37605939 None:intergenic 50.0%
CGCAAGAAAGCAGCACCAAT+TGG - chr7.4:37605255-37605274 None:intergenic 50.0%
CTGTCGGCAGTAATCTTCCA+TGG - chr7.4:37604386-37604405 None:intergenic 50.0%
TGGAGACCTACCATCTGATG+AGG + chr7.4:37604945-37604964 MS.gene055035:CDS 50.0%
!! TCGCACACGAAGTACTGCAA+GGG - chr7.4:37604436-37604455 None:intergenic 50.0%
CAACACTCCTCCTAAGGGGA+AGG - chr7.4:37605073-37605092 None:intergenic 55.0%
TGTGTATGCTGAGGCTGGGA+AGG + chr7.4:37604168-37604187 MS.gene055035:CDS 55.0%
!! CTCGCACACGAAGTACTGCA+AGG - chr7.4:37604437-37604456 None:intergenic 55.0%
GCGCCTGCCCCGTAACATAC+AGG + chr7.4:37605914-37605933 MS.gene055035:CDS 65.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 37604085 37606615 37604085 ID=MS.gene055035
chr7.4 mRNA 37604085 37606615 37604085 ID=MS.gene055035.t1;Parent=MS.gene055035
chr7.4 exon 37604085 37604477 37604085 ID=MS.gene055035.t1.exon1;Parent=MS.gene055035.t1
chr7.4 CDS 37604085 37604477 37604085 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
chr7.4 exon 37604589 37604729 37604589 ID=MS.gene055035.t1.exon2;Parent=MS.gene055035.t1
chr7.4 CDS 37604589 37604729 37604589 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
chr7.4 exon 37604829 37605575 37604829 ID=MS.gene055035.t1.exon3;Parent=MS.gene055035.t1
chr7.4 CDS 37604829 37605575 37604829 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
chr7.4 exon 37605648 37605728 37605648 ID=MS.gene055035.t1.exon4;Parent=MS.gene055035.t1
chr7.4 CDS 37605648 37605728 37605648 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
chr7.4 exon 37605855 37605935 37605855 ID=MS.gene055035.t1.exon5;Parent=MS.gene055035.t1
chr7.4 CDS 37605855 37605935 37605855 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
chr7.4 exon 37606541 37606615 37606541 ID=MS.gene055035.t1.exon6;Parent=MS.gene055035.t1
chr7.4 CDS 37606541 37606615 37606541 ID=cds.MS.gene055035.t1;Parent=MS.gene055035.t1
Gene Sequence

>MS.gene055035

ATGGCTTCCATTCAAAGTGAATCAGTTGAACAAGTTGATAATGTTCGTCTGAGAGTTTTGGTGGACAAAGAAAAGAACAAAGTTGTGTATGCTGAGGCTGGGAAGGATTTCGTTGATGCTCTCTTCAGCTTCTTAACATTGCCTTTAGGGACTATTGCAAGACTCGTTGCTAAGGACTCAAATATTGAAGAAGTGAAATTTGGCAGCATTAGCACATTGTATCAAAGTGTGTCAGATCTTGATCAACAATATTTGTGCTCACGTACATGCAAGGAAATGCTACTGCAACCAAGAAACTCCATGGAAGATTACTGCCGACAGCTGAAACTAAATATAGACGACACAGAACCCTTGCAGTACTTCGTGTGCGAGAATTGGGATTGTAGAAGAAAGGTAAGTGGATGCCTTTTGAGCTTTTTTAGGAACCAAAAATGCTATTGTGGGAAAGTAATGAACAGAGAAGTATTCCCGAAGTTTTTCAATATAGAAAATGGTTTTGTGAAGGAAAATGCTACTTTTATCATTTGTGATGATCTTTGTGTTTTGCCTAATGTTTTTGGAGCAAGTTTAAATCTACTTCAAAAGCTTGGAGTCGAGACTATTGATGCTATTGAGGAACAAACTGTTGACATCAACAAGAAAGAGGCATGCTTTTTTCGGCTTCTATATCATCTACATTACATATATCATCTTTGAATATTGATTATTTTTTCTAATTGAATATTTCTTATTCGATTTGTATAGGTAGTTGATCTTCTTAAGTTATCATTAATCTCAATGACCCCTTTGACCAATTTTATTTTACGAAAGAGCCAATCTGTTGATAATTTGAAACTAAGAAACAATATGGAGTTTCTGATTGGAGACCTACCATCTGATGAGGATAGACAAATGTCTGTGAAAGTGACATTAAGAAAATCGAATGAACAAATTCTGTTTGTCGAAGCAGGAGATGATTTTATTGACTTTGTTTTTAGTTTTCTAACCTTCCCCTTAGGAGGAGTGTTGCATATGCTTCAAGGATTTTCGTCTTTAAGCAGCATTGGTAACTTGTACAAGAGCTTTTCTGATTTGAGTCCGGACATATATTTAATGTCTGAGGGACTCAAAGACAAACTATGTAAGCCTCTCATTACCCCAGAATTTGAACTCAAGAACCAGATATTACCAATTGGTGCTGCTTTCTTGCGTGTGAATTATTCTCATACTTACTATGATTACACAAGGCAGAGGTATCCTGACCTTACAAAAGAAATGGTTTATTCTAACTATAATAGAATTTATGAAGAAAAAAATGCACCATTTAAATTGGTTGATCCCAAGTCTTCTATGGCAAAATCATCATATGCCAAAGGACCATCAATGTATATGGTGACAGACGACTTGTGTGTGAGTCCAATGTCGTCCATTTCTACTATGTCATTTCTAAAAAGATTTAAAGTCCCTCTACATGATTTAGAGGAAAGAGTTATCAAAATTGGCGTCAAGGAGGTATGCACATGTTCATCTTGTAATTGTTTTTTATTTGCTAACACTACACTTACTTTTTGTTACATGTCACAGGGTCTCAGCCTACTTAAAGCTTCATTGGAATCAACATCTGCTTTGAAAAATGGTCTCAGCCAATTTATAAGAACTGTTAAGGTGGAGAAATGCCTAAATATTTTCCTTATCAAGTTTAGATTACTCTCTTCTCCATAATAGGGTTATTCCGTGCTAAAGATATACTAACCTAACCAACTTACTTTTGTTGATGATATGAACATGCAGGTCTCAACTTCTGCTCCTGAAGATGGGGCTAAACCAAAATCTACTTTGGTGGAGGAATTGCGCCTGCCCCGTAACATACAGGTATTACTATTTATGGTTTAATTACATCTTTGTTGATATGTTTAACCGAATCCTCTATTTGATGGAGCCATGCATCAATATTGCAAAAAAGTTTAGTTTGTGACTCAATACATAATACTCCAATATGTCAAACCCTTGTTGACTTTTTGCTTCCTAAACAATTTTTCATTTCTTTCATGGATTTTTACATTCATTATTATGTTTTTTGAGATTAGGCGTGTCTTCATGTCTTACATGCATCAATATACGACACTTGCATTCACACAAGTCCGACAGTTCAAATGATGTCTCATTTTTTTCCTTTACTTCACACGTTATATATTCTTTAGACAGATCTTTAGACATATCTCTATGAGATTTAACGATGTGTATAAGCAACAATACTATGAGATTTAAAGATGTGTAGAAGCAACGATGAACACTAACAAAGGTTGAAAACACATTAAATACATGTGTATAATTTGATTAACAATATAGGTAGTTTTGGAATAATGTTTGTCTTTTATTTAGTGCCAGTGTTGCTATTGTTATGTATTTGTAGTGTTGATATGTCAGTATCTATATCTTTTTGGAAGAGGAAGCTAACACTGGTATTTTCAATTCAGGAAAAAGAGAACAAGACTCCTGCATTGGCTTTGCAAAGATCAACTAGGAAACGCAAAGCTAGCGATCATGCTTGA
Protein sequence

>MS.gene055035.t1

MASIQSESVEQVDNVRLRVLVDKEKNKVVYAEAGKDFVDALFSFLTLPLGTIARLVAKDSNIEEVKFGSISTLYQSVSDLDQQYLCSRTCKEMLLQPRNSMEDYCRQLKLNIDDTEPLQYFVCENWDCRRKENATFIICDDLCVLPNVFGASLNLLQKLGVETIDAIEEQTVDINKKEVVDLLKLSLISMTPLTNFILRKSQSVDNLKLRNNMEFLIGDLPSDEDRQMSVKVTLRKSNEQILFVEAGDDFIDFVFSFLTFPLGGVLHMLQGFSSLSSIGNLYKSFSDLSPDIYLMSEGLKDKLCKPLITPEFELKNQILPIGAAFLRVNYSHTYYDYTRQRYPDLTKEMVYSNYNRIYEEKNAPFKLVDPKSSMAKSSYAKGPSMYMVTDDLCVSPMSSISTMSFLKRFKVPLHDLEERVIKIGVKEGLSLLKASLESTSALKNGLSQFIRTVKVSTSAPEDGAKPKSTLVEELRLPRNIQEKENKTPALALQRSTRKRKASDHA