AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99474

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99474.t1 MTR_4g019340 88.163 490 32 3 11 497 37 503 0.0 857
MS.gene99474.t1 MTR_4g017830 74.535 538 127 4 1 536 1 530 0.0 786
MS.gene99474.t1 MTR_4g017440 66.931 505 150 6 4 503 6 498 0.0 649
MS.gene99474.t1 MTR_4g017410 64.818 523 147 9 1 516 1 493 0.0 637
MS.gene99474.t1 MTR_4g017470 61.568 523 150 6 1 516 1 479 0.0 594
MS.gene99474.t1 MTR_4g017550 64.979 474 143 10 39 507 18 473 0.0 572
MS.gene99474.t1 MTR_4g017550 29.457 258 134 11 6 245 242 469 1.83e-11 66.6
MS.gene99474.t1 MTR_4g017610 60.040 503 170 9 2 503 3 475 0.0 566
MS.gene99474.t1 MTR_4g019310 42.308 520 249 15 1 503 1 486 4.80e-117 355
MS.gene99474.t1 MTR_7g066340 34.182 550 273 15 1 536 1 475 1.09e-83 268
MS.gene99474.t1 MTR_7g066260 34.918 547 278 16 5 536 8 491 2.68e-83 268
MS.gene99474.t1 MTR_7g066570 36.069 524 272 16 5 511 7 484 8.25e-81 263
MS.gene99474.t1 MTR_7g058710 32.957 531 290 12 1 513 1 483 1.33e-77 252
MS.gene99474.t1 MTR_7g066350 34.156 527 249 14 8 510 5 457 4.76e-77 250
MS.gene99474.t1 MTR_7g066350 29.134 254 145 8 274 516 9 238 1.81e-16 82.4
MS.gene99474.t1 MTR_7g066410 33.457 541 279 14 13 509 11 514 1.48e-74 245
MS.gene99474.t1 MTR_7g066430 35.433 508 288 11 13 507 5 485 3.74e-74 244
MS.gene99474.t1 MTR_7g066400 33.523 528 245 14 1 510 1 440 5.37e-71 234
MS.gene99474.t1 MTR_7g012680 34.528 530 247 17 1 511 1 449 7.16e-71 234
MS.gene99474.t1 MTR_1g041315 33.462 517 252 18 1 491 1 451 1.71e-69 231
MS.gene99474.t1 MTR_7g066360 33.333 549 280 15 2 537 4 479 3.74e-66 222
MS.gene99474.t1 MTR_7g062280 33.087 541 284 17 1 513 1 491 1.17e-63 216
MS.gene99474.t1 MTR_7g063880 32.762 525 282 17 1 512 1 467 3.03e-63 214
MS.gene99474.t1 MTR_7g066310 30.467 535 224 14 13 536 8 405 1.57e-60 205
MS.gene99474.t1 MTR_7g063900 33.591 518 297 15 1 507 1 482 7.64e-60 206
MS.gene99474.t1 MTR_7g063660 32.554 513 289 16 13 513 10 477 1.85e-59 204
MS.gene99474.t1 MTR_7g066580 31.089 505 269 13 18 507 1 441 2.51e-59 203
MS.gene99474.t1 MTR_7g062300 32.495 517 293 13 1 506 1 472 3.08e-59 204
MS.gene99474.t1 MTR_7g066290 37.008 381 197 12 3 365 6 361 1.29e-58 201
MS.gene99474.t1 MTR_7g063840 31.660 518 294 17 7 513 5 473 3.32e-57 198
MS.gene99474.t1 MTR_2g029400 30.847 496 233 11 8 495 10 403 1.77e-53 186
MS.gene99474.t1 MTR_4g019240 76.613 124 24 4 1 122 1 121 1.33e-52 177
MS.gene99474.t1 MTR_4g019240 85.915 71 10 0 465 535 112 182 4.87e-29 113
MS.gene99474.t1 MTR_7g063820 32.580 531 293 19 1 513 1 484 2.08e-52 186
MS.gene99474.t1 MTR_7g063920 30.250 519 273 15 1 513 1 436 1.10e-51 182
MS.gene99474.t1 MTR_7g063890 31.086 534 293 17 6 514 22 505 3.50e-49 177
MS.gene99474.t1 MTR_7g063830 29.434 530 271 16 1 513 1 444 9.74e-47 169
MS.gene99474.t1 MTR_7g063670 30.443 519 278 18 6 513 11 457 2.70e-44 163
MS.gene99474.t1 MTR_3g065200 28.024 496 266 14 13 501 4 415 7.71e-42 156
MS.gene99474.t1 MTR_7g058700 34.844 353 164 15 24 356 531 837 8.03e-41 158
MS.gene99474.t1 MTR_3g007740 29.207 517 281 18 1 507 1 442 1.01e-40 153
MS.gene99474.t1 MTR_7g066480 34.605 367 181 12 13 357 17 346 2.43e-40 151
MS.gene99474.t1 MTR_7g066480 35.922 103 55 1 416 507 319 421 8.43e-11 64.7
MS.gene99474.t1 MTR_7g066280 27.391 460 264 13 99 536 1 412 3.06e-38 145
MS.gene99474.t1 MTR_7g066280 26.894 264 142 8 7 243 145 384 3.30e-13 72.0
MS.gene99474.t1 MTR_7g066490 32.222 360 185 10 13 357 17 332 1.24e-35 138
MS.gene99474.t1 MTR_7g066680 31.077 325 162 9 220 503 31 334 8.30e-28 114
MS.gene99474.t1 MTR_1g014060 33.191 235 123 8 21 245 9 219 3.80e-26 110
MS.gene99474.t1 MTR_1g014060 36.232 207 97 5 304 507 210 384 9.83e-22 97.8
MS.gene99474.t1 MTR_4g019350 52.000 100 37 2 332 431 1 89 6.34e-24 96.3
MS.gene99474.t1 MTR_7g063720 30.894 246 134 5 270 512 19 231 7.84e-23 97.8
MS.gene99474.t1 MTR_1g115440 30.859 256 149 8 1 247 1 237 1.13e-21 94.7
MS.gene99474.t1 MTR_1g115440 31.535 241 134 8 274 501 11 233 1.18e-19 88.6
MS.gene99474.t1 MTR_8g445860 28.968 252 123 6 274 501 13 232 2.51e-21 93.6
MS.gene99474.t1 MTR_8g445860 30.290 241 150 5 10 247 11 236 2.46e-20 90.5
MS.gene99474.t1 MTR_7g063850 26.625 323 193 9 198 513 3 288 2.05e-20 92.0
MS.gene99474.t1 MTR_7g100420 33.096 281 164 8 226 503 134 393 2.15e-20 94.0
MS.gene99474.t1 MTR_7g062270 34.146 205 102 7 1 197 1 180 1.28e-19 91.3
MS.gene99474.t1 MTR_0150s0010 30.932 236 142 6 274 501 10 232 5.14e-19 87.0
MS.gene99474.t1 MTR_0150s0010 28.631 241 151 4 13 247 11 236 4.06e-18 84.3
MS.gene99474.t1 MTR_8g023240 23.416 363 222 10 6 353 31 352 4.18e-18 87.8
MS.gene99474.t1 MTR_7g066390 43.137 102 55 2 435 536 50 148 1.28e-13 68.9
MS.gene99474.t1 MTR_0150s0030 35.294 102 57 2 274 372 8 103 1.30e-13 70.5
MS.gene99474.t1 MTR_8g445910 36.275 102 56 2 274 372 8 103 1.89e-13 71.2
MS.gene99474.t1 MTR_1g115450 40.506 79 45 1 274 350 7 85 1.26e-12 65.9
MS.gene99474.t1 MTR_1g115450 34.188 117 64 1 10 126 5 108 6.70e-12 63.5
MS.gene99474.t1 MTR_8g044880 36.275 102 56 2 274 372 8 103 1.27e-12 68.9
MS.gene99474.t1 MTR_1g115455 32.787 122 65 2 13 130 10 118 1.67e-12 67.8
MS.gene99474.t1 MTR_1g115455 37.975 79 47 1 274 350 9 87 6.37e-11 63.2
MS.gene99474.t1 MTR_6g006800 63.830 47 16 1 1 47 1 46 4.29e-12 62.0
MS.gene99474.t1 MTR_8g445920 40.909 66 39 0 274 339 10 75 3.06e-11 60.8
MS.gene99474.t1 MTR_8g044890 40.909 66 39 0 274 339 10 75 3.06e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99474.t1 AT5G01120 31.801 544 313 17 9 536 6 507 2.48e-63 216
MS.gene99474.t1 AT5G01130 32.213 506 272 15 2 504 4 441 1.67e-61 209
MS.gene99474.t1 AT3G09140 30.769 533 282 13 9 503 6 489 1.41e-60 208
MS.gene99474.t1 AT3G09140 27.459 244 159 9 270 500 5 243 7.65e-13 71.2
MS.gene99474.t1 AT5G43240 32.190 525 277 17 13 503 10 489 5.03e-58 201
MS.gene99474.t1 AT5G43240 32.190 525 277 17 13 503 10 489 5.03e-58 201
MS.gene99474.t1 AT5G43240 32.190 525 277 17 13 503 10 489 5.03e-58 201
MS.gene99474.t1 AT5G01140 31.434 544 289 17 2 536 4 472 3.75e-56 196
MS.gene99474.t1 AT3G09140 30.481 561 275 16 9 503 6 517 3.17e-55 194
MS.gene99474.t1 AT5G01150 30.561 553 310 17 2 536 4 500 1.10e-54 192
MS.gene99474.t1 AT3G09110 34.125 337 188 8 9 335 6 318 8.70e-47 167
MS.gene99474.t1 AT5G37320 29.389 507 301 14 19 510 18 482 9.13e-46 167
MS.gene99474.t1 AT3G09120 33.232 328 194 6 9 328 3 313 4.07e-45 161
MS.gene99474.t1 AT3G09120 29.536 237 145 9 274 501 6 229 1.98e-14 74.7
MS.gene99474.t1 AT5G43240 29.925 401 207 13 166 536 11 367 1.47e-28 117

Find 108 sgRNAs with CRISPR-Local

Find 300 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAAACAGCCTAAGAATT+TGG 0.203171 4.2:+83195312 MS.gene99474:CDS
AACGATTCACATGGATTCTT+TGG 0.212815 4.2:-83195169 None:intergenic
GAGTATAATTATAGTTATTA+TGG 0.232690 4.2:+83198194 MS.gene99474:CDS
TGGCGGGTTGTCTTTGTTCT+TGG 0.234472 4.2:-83197790 None:intergenic
TTGCACCCGCGACATTAAAA+TGG 0.255172 4.2:+83197845 MS.gene99474:CDS
GGTATTGCTGATGCTTTAAA+TGG 0.278450 4.2:+83195361 MS.gene99474:CDS
AACTTTGTTGACTTCATGTT+TGG 0.286637 4.2:+83197927 MS.gene99474:CDS
CATGCAGATGTTGATGGAAT+TGG 0.293472 4.2:+83195462 MS.gene99474:CDS
ATAACTCAATCTTCTAGTTT+AGG 0.298482 4.2:-83199234 None:intergenic
CCACTCACTACATATTATTA+TGG 0.320058 4.2:+83198152 MS.gene99474:CDS
GGCTTCCTTACAATACCATT+AGG 0.340547 4.2:+83197948 MS.gene99474:CDS
AGGGTCCATTGTAAAGGTTT+TGG 0.343635 4.2:+83197968 MS.gene99474:CDS
GCTTCCTTACAATACCATTA+GGG 0.360487 4.2:+83197949 MS.gene99474:CDS
ACTGCTGATGCAAACTGGTT+TGG 0.367245 4.2:-83197810 None:intergenic
ACAGGGTTCCAAACATCATT+TGG 0.367250 4.2:-83195124 None:intergenic
GAAGATTGTGCCAAGTTCTT+TGG 0.367844 4.2:+83195432 MS.gene99474:CDS
AGCAAAAGCCTTGACTGAAA+TGG 0.377248 4.2:+83198283 MS.gene99474:CDS
GAATTTGGATTCTGAGGGTC+AGG 0.378169 4.2:+83195327 MS.gene99474:CDS
CCCTCACTCCAAATGATGTT+TGG 0.379098 4.2:+83195116 MS.gene99474:CDS
CCCTCAGAATCCAAATTCTT+AGG 0.386443 4.2:-83195322 None:intergenic
CTTTGACTGCTGATGCAAAC+TGG 0.388699 4.2:-83197815 None:intergenic
CACACGCAACATTGGAAAAC+AGG 0.390766 4.2:+83195516 MS.gene99474:CDS
GGAATGTTGAGGGGCTGGTT+TGG 0.395287 4.2:-83198116 None:intergenic
ATGCAGATGTTGATGGAATT+GGG 0.399870 4.2:+83195463 MS.gene99474:CDS
CTATAATTATACTCTTGCTT+TGG 0.402657 4.2:-83198185 None:intergenic
TTCTGATTTGAGTCAATTAG+AGG 0.405222 4.2:+83195489 MS.gene99474:CDS
GTATTGCTGATGCTTTAAAT+GGG 0.415851 4.2:+83195362 MS.gene99474:CDS
CAGAGGCTCATGAGAGGTTA+AGG 0.420057 4.2:-83197742 None:intergenic
ATTCTTAGGCTGTTTATTAG+TGG 0.429316 4.2:-83195308 None:intergenic
TTCTTAGGCTGTTTATTAGT+GGG 0.434937 4.2:-83195307 None:intergenic
AACGGTAGAATTAAAGATAG+AGG 0.436841 4.2:+83198223 MS.gene99474:CDS
CAGAATCTTGATTGTACTTG+TGG 0.438965 4.2:+83195280 MS.gene99474:CDS
ACTAGTTTGAACACACTTTG+AGG 0.444744 4.2:-83199187 None:intergenic
CGTTGTTGTGTGTGGTTAAG+AGG 0.456967 4.2:-83195018 None:intergenic
ACCATTAGGGTCCATTGTAA+AGG 0.460196 4.2:+83197962 MS.gene99474:CDS
TCTGAGGGTCAGGGGAATAG+TGG 0.460813 4.2:+83195337 MS.gene99474:CDS
CGCTCCATGTTACTCAATCC+TGG 0.462371 4.2:+83198071 MS.gene99474:CDS
ACCTTTACAATGGACCCTAA+TGG 0.477568 4.2:-83197963 None:intergenic
AACAACATTTGTTTGCAGAC+AGG 0.485916 4.2:-83195142 None:intergenic
CATGAGGAATGTTGAGGGGC+TGG 0.489637 4.2:-83198121 None:intergenic
GCTGATGCAAACTGGTTTGG+CGG 0.490283 4.2:-83197807 None:intergenic
GACTTCCATGCAGATGTTGA+TGG 0.493543 4.2:+83195456 MS.gene99474:CDS
CCAAACATCATTTGGAGTGA+GGG 0.493650 4.2:-83195116 None:intergenic
GCCTAAGAATTTGGATTCTG+AGG 0.494703 4.2:+83195321 MS.gene99474:CDS
ACAACATTTGTTTGCAGACA+GGG 0.497506 4.2:-83195141 None:intergenic
CAAAGTGTGTTCAAACTAGT+AGG 0.500953 4.2:+83199190 MS.gene99474:CDS
ATGGAAGTCACCAAAGAACT+TGG 0.501440 4.2:-83195442 None:intergenic
GTACAAGAGTGTAGAGAGCT+TGG 0.505497 4.2:+83198025 MS.gene99474:CDS
TCTTGGGTCCATTTCAGTCA+AGG 0.507464 4.2:-83198291 None:intergenic
ACTACATATTATTATGGTAC+AGG 0.518635 4.2:+83198158 MS.gene99474:CDS
TTCAAAATTGTATTTGTCAG+AGG 0.520812 4.2:-83197759 None:intergenic
TTAATTCTTTCAAATATGAA+AGG 0.523699 4.2:-83198418 None:intergenic
TGGACCCTAATGGTATTGTA+AGG 0.526033 4.2:-83197953 None:intergenic
TCAAAGTGTGGTGCGACACC+AGG 0.529733 4.2:-83198089 None:intergenic
TAGTAGCAAGTGGAAGAGAA+AGG 0.530533 4.2:-83194988 None:intergenic
AGGCAAGAATTAGCAGAAAG+TGG 0.533853 4.2:-83198398 None:intergenic
CCTTGTTGATCTTGAAGTGA+AGG 0.535507 4.2:+83198448 MS.gene99474:CDS
TCAAAATTTACACTGACTAG+TGG 0.539589 4.2:+83199120 MS.gene99474:CDS
TGAACATTGATGACACTGAA+TGG 0.541744 4.2:+83195209 MS.gene99474:CDS
CTGATGCAAACTGGTTTGGC+GGG 0.543289 4.2:-83197806 None:intergenic
CTTCAAGATCAACAAGGGGA+AGG 0.544062 4.2:-83198442 None:intergenic
ATTTACACTGACTAGTGGAC+TGG 0.547720 4.2:+83199125 MS.gene99474:CDS
TAGAGAGCTTGGACTCTTCG+TGG 0.547747 4.2:+83198036 MS.gene99474:CDS
GAGGAAGTCACACGCAACAT+TGG 0.558335 4.2:+83195508 MS.gene99474:CDS
TAGTTATTATGGTTATCAAA+CGG 0.564215 4.2:+83198205 MS.gene99474:CDS
TCCAAACATCATTTGGAGTG+AGG 0.565006 4.2:-83195117 None:intergenic
TCAGAATCATTATCATTCCT+CGG 0.566842 4.2:+83195061 MS.gene99474:CDS
GGAGTGATTTCTAAGTCGCA+TGG 0.571812 4.2:+83198248 MS.gene99474:CDS
GTAGAATTAAAGATAGAGGG+AGG 0.580072 4.2:+83198227 MS.gene99474:CDS
ATTTGGATTCTGAGGGTCAG+GGG 0.580968 4.2:+83195329 MS.gene99474:CDS
ACGGTAGAATTAAAGATAGA+GGG 0.581849 4.2:+83198224 MS.gene99474:CDS
ACCATCCAAAACCTTTACAA+TGG 0.583435 4.2:-83197973 None:intergenic
CCTAAGAATTTGGATTCTGA+GGG 0.584987 4.2:+83195322 MS.gene99474:CDS
TCTCCTACACTATATAATGC+CGG 0.585136 4.2:-83198359 None:intergenic
GAAGTCAACAAAGTTTCCAC+TGG 0.587458 4.2:-83197919 None:intergenic
TTGATCATCGTTGTTGTGTG+TGG 0.590857 4.2:-83195026 None:intergenic
GAGGAATGATAATGATTCTG+AGG 0.600918 4.2:-83195059 None:intergenic
CCTTCACTTCAAGATCAACA+AGG 0.610416 4.2:-83198448 None:intergenic
AATTTGGATTCTGAGGGTCA+GGG 0.610725 4.2:+83195328 MS.gene99474:CDS
CAATTCCATCAACATCTGCA+TGG 0.613002 4.2:-83195461 None:intergenic
TATTGCTGATGCTTTAAATG+GGG 0.614508 4.2:+83195363 MS.gene99474:CDS
AGGTTAAGGTGTACTTGAGG+AGG 0.616375 4.2:-83197728 None:intergenic
AGGCGAATGATAGTAGCAAG+TGG 0.618314 4.2:-83194998 None:intergenic
ATGTACATGAGGAATGTTGA+GGG 0.622021 4.2:-83198126 None:intergenic
GTGAGTGGTGGATGTACATG+AGG 0.635076 4.2:-83198137 None:intergenic
GGGTTTGTACGCTTTCATAG+AGG 0.642551 4.2:-83195096 None:intergenic
TTGATGTCAAGGAAGCTGCA+AGG 0.648287 4.2:-83199101 None:intergenic
GATGGGATTTGTGAAGAGAC+CGG 0.651602 4.2:+83198340 MS.gene99474:CDS
CTTCACTTCAAGATCAACAA+GGG 0.654366 4.2:-83198447 None:intergenic
GAAGTGAAGGTGATATGCAT+TGG 0.654807 4.2:+83198461 MS.gene99474:CDS
TAATAATATGTAGTGAGTGG+TGG 0.656097 4.2:-83198149 None:intergenic
TGCAGATGTTGATGGAATTG+GGG 0.656452 4.2:+83195464 MS.gene99474:CDS
AGACCGGCATTATATAGTGT+AGG 0.657855 4.2:+83198356 MS.gene99474:CDS
TGGTTTGGACAATCAAAGTG+TGG 0.662188 4.2:-83198101 None:intergenic
GAGGGTCAGGGGAATAGTGG+TGG 0.663204 4.2:+83195340 MS.gene99474:CDS
GAGAGGTTAAGGTGTACTTG+AGG 0.667782 4.2:-83197731 None:intergenic
TTGTCTATGAAGATTCTGAG+AGG 0.671175 4.2:-83194902 None:intergenic
ATAATCTGTGCAGAAGCCAG+TGG 0.673897 4.2:+83197903 MS.gene99474:CDS
ATTTGTCAGAGGCTCATGAG+AGG 0.675408 4.2:-83197748 None:intergenic
TTCACTTCAAGATCAACAAG+GGG 0.676098 4.2:-83198446 None:intergenic
TTCTTGCATAACGATTCACA+TGG 0.676577 4.2:-83195178 None:intergenic
GATGTACATGAGGAATGTTG+AGG 0.679220 4.2:-83198127 None:intergenic
ACGCAACATTGGAAAACAGG+AGG 0.693923 4.2:+83195519 MS.gene99474:CDS
GATATGCATTGGTGAAGCTG+AGG 0.712892 4.2:+83198472 MS.gene99474:CDS
ATGTCAAGGAAGCTGCAAGG+AGG 0.721178 4.2:-83199098 None:intergenic
CCATAATAATATGTAGTGAG+TGG 0.727149 4.2:-83198152 None:intergenic
GACACCAGGATTGAGTAACA+TGG 0.730789 4.2:-83198075 None:intergenic
TGTACATGAGGAATGTTGAG+GGG 0.757600 4.2:-83198125 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAAGAATGTTATAT+TGG + chr4.2:83197637-83197656 MS.gene99474:intron 10.0%
!!! CAATTTATTTTTTTAAAACT+CGG - chr4.2:83195896-83195915 None:intergenic 10.0%
!!! TTTTAAAAGAATGTTATATT+GGG + chr4.2:83197638-83197657 MS.gene99474:intron 10.0%
!! AAATTAAAAAAAGATGTGAT+GGG + chr4.2:83198323-83198342 MS.gene99474:CDS 15.0%
!! GAAATAATATGTTTAGATAT+AGG + chr4.2:83198861-83198880 MS.gene99474:intron 15.0%
!! GAGTATAATTATAGTTATTA+TGG + chr4.2:83198194-83198213 MS.gene99474:CDS 15.0%
!! TAATAAAATTATTGGACTTA+TGG - chr4.2:83198683-83198702 None:intergenic 15.0%
!! TCAAAATAACTAATGAATAA+TGG + chr4.2:83196257-83196276 MS.gene99474:intron 15.0%
!! TTAATTCTTTCAAATATGAA+AGG - chr4.2:83198421-83198440 None:intergenic 15.0%
!! TTTAAAAGAATGTTATATTG+GGG + chr4.2:83197639-83197658 MS.gene99474:intron 15.0%
!!! ACATCTTTTTTTAATTTGTT+GGG - chr4.2:83198320-83198339 None:intergenic 15.0%
!!! TTTATAACTTTCGTTTTTTA+GGG + chr4.2:83196425-83196444 MS.gene99474:intron 15.0%
!! AATATGTTTAGATATAGGTT+GGG + chr4.2:83198866-83198885 MS.gene99474:intron 20.0%
!! AATTCAATACCTCAAATTAT+AGG - chr4.2:83196334-83196353 None:intergenic 20.0%
!! ATACAAGATATTAAACCAAA+CGG - chr4.2:83197584-83197603 None:intergenic 20.0%
!! ATAGAGTACAATAGATTTAA+GGG - chr4.2:83196715-83196734 None:intergenic 20.0%
!! ATTAGTTCGATAACTAAATA+AGG - chr4.2:83196909-83196928 None:intergenic 20.0%
!! CAAATTAAAAAAAGATGTGA+TGG + chr4.2:83198322-83198341 MS.gene99474:CDS 20.0%
!! CAGAATAATTAGTGTAAAAA+TGG - chr4.2:83197013-83197032 None:intergenic 20.0%
!! TAATATGTTTAGATATAGGT+TGG + chr4.2:83198865-83198884 MS.gene99474:intron 20.0%
!! TAGTTATTATGGTTATCAAA+CGG + chr4.2:83198205-83198224 MS.gene99474:CDS 20.0%
!! TAGTTGTTGTACTTAAATTA+CGG + chr4.2:83197197-83197216 MS.gene99474:intron 20.0%
!! TTAGACAACAAATAAACAAA+AGG - chr4.2:83198535-83198554 None:intergenic 20.0%
!! TTAGTTCGATAACTAAATAA+GGG - chr4.2:83196908-83196927 None:intergenic 20.0%
!!! ATCTTTTTTTAATTTGTTGG+GGG - chr4.2:83198318-83198337 None:intergenic 20.0%
!!! CACATCTTTTTTTAATTTGT+TGG - chr4.2:83198321-83198340 None:intergenic 20.0%
!!! CATCTTTTTTTAATTTGTTG+GGG - chr4.2:83198319-83198338 None:intergenic 20.0%
!!! CTTTATAACTTTCGTTTTTT+AGG + chr4.2:83196424-83196443 MS.gene99474:intron 20.0%
!!! TATAATTTGAGGTATTGAAT+TGG + chr4.2:83196333-83196352 MS.gene99474:intron 20.0%
!!! TTGTTATTGTAGTAAAATGA+GGG + chr4.2:83196001-83196020 MS.gene99474:intron 20.0%
! AAACATTCACAATGTTATGA+TGG + chr4.2:83197161-83197180 MS.gene99474:intron 25.0%
! AAATTATTGGACTTATGGAA+AGG - chr4.2:83198678-83198697 None:intergenic 25.0%
! AATAGAAAGTGATAAACTCT+AGG - chr4.2:83196625-83196644 None:intergenic 25.0%
! ACTACATATTATTATGGTAC+AGG + chr4.2:83198158-83198177 MS.gene99474:CDS 25.0%
! AGAAAAAGATTTAACCTTCA+AGG + chr4.2:83196810-83196829 MS.gene99474:intron 25.0%
! ATAACTAAATAAGGGATGTA+AGG - chr4.2:83196900-83196919 None:intergenic 25.0%
! ATCATCTGTTAAGAATAGAA+AGG + chr4.2:83196465-83196484 MS.gene99474:intron 25.0%
! CATTTGTATGTATGTTATCT+TGG + chr4.2:83197663-83197682 MS.gene99474:intron 25.0%
! CTATAATTATACTCTTGCTT+TGG - chr4.2:83198188-83198207 None:intergenic 25.0%
! GAAAAAGATTTAACCTTCAA+GGG + chr4.2:83196811-83196830 MS.gene99474:intron 25.0%
! GATAGAGTACAATAGATTTA+AGG - chr4.2:83196716-83196735 None:intergenic 25.0%
! TAACAATGTCCTATAATTTG+AGG + chr4.2:83196322-83196341 MS.gene99474:intron 25.0%
! TAACTAAATAAGGGATGTAA+GGG - chr4.2:83196899-83196918 None:intergenic 25.0%
! TAAGAATAGAAAGGAAAGTA+AGG + chr4.2:83196474-83196493 MS.gene99474:intron 25.0%
! TAATAAACAGCCTAAGAATT+TGG + chr4.2:83195312-83195331 MS.gene99474:CDS 25.0%
! TAATTTGAGTGAAGAGTTAA+AGG + chr4.2:83197450-83197469 MS.gene99474:intron 25.0%
! TACAAACATGTTATGAAACA+CGG - chr4.2:83195697-83195716 None:intergenic 25.0%
! TAGTGTACCATAAATGAAAA+GGG + chr4.2:83197123-83197142 MS.gene99474:intron 25.0%
! TATAAAAAATGTAGTGTACC+TGG - chr4.2:83198786-83198805 None:intergenic 25.0%
! TATCAATTAGGAACAATTTG+AGG - chr4.2:83196955-83196974 None:intergenic 25.0%
! TTAGTGTACCATAAATGAAA+AGG + chr4.2:83197122-83197141 MS.gene99474:intron 25.0%
! TTCAAAATTGTATTTGTCAG+AGG - chr4.2:83197762-83197781 None:intergenic 25.0%
!! ATGTTTTCCCTTTTCATTTA+TGG - chr4.2:83197133-83197152 None:intergenic 25.0%
!! CTCATTTTACTACAATAACA+AGG - chr4.2:83196002-83196021 None:intergenic 25.0%
!! GAAGGTTAAATCTTTTTCTT+TGG - chr4.2:83196809-83196828 None:intergenic 25.0%
!! TTTAAATGGGGTTTTTGTTA+AGG + chr4.2:83195375-83195394 MS.gene99474:CDS 25.0%
!!! CGCAGTTTCAAATATTTTTT+CGG - chr4.2:83195934-83195953 None:intergenic 25.0%
!!! CTTGTTATTGTAGTAAAATG+AGG + chr4.2:83196000-83196019 MS.gene99474:intron 25.0%
!!! TTTTTAGGGTAGTTTGTATA+TGG + chr4.2:83196439-83196458 MS.gene99474:intron 25.0%
!!! TTTTTTTAAAACTCGGTATC+TGG - chr4.2:83195889-83195908 None:intergenic 25.0%
AACCTTAAAGGGAATAAGTT+GGG + chr4.2:83196738-83196757 MS.gene99474:intron 30.0%
AACGGTAGAATTAAAGATAG+AGG + chr4.2:83198223-83198242 MS.gene99474:CDS 30.0%
AATGTAGTAGTAGGGAATTA+GGG + chr4.2:83196547-83196566 MS.gene99474:intron 30.0%
ACGGTAGAATTAAAGATAGA+GGG + chr4.2:83198224-83198243 MS.gene99474:CDS 30.0%
AGAAACAGAATGTAGTAGTA+GGG + chr4.2:83196539-83196558 MS.gene99474:intron 30.0%
ATTCTTAGGCTGTTTATTAG+TGG - chr4.2:83195311-83195330 None:intergenic 30.0%
CCACTCACTACATATTATTA+TGG + chr4.2:83198152-83198171 MS.gene99474:CDS 30.0%
CCATAATAATATGTAGTGAG+TGG - chr4.2:83198155-83198174 None:intergenic 30.0%
CCTAATTGATATCATAAGCT+GGG + chr4.2:83196964-83196983 MS.gene99474:intron 30.0%
GCAATATTGGAACGAATATT+TGG - chr4.2:83197257-83197276 None:intergenic 30.0%
GGTACACTAATAACTTTATG+TGG - chr4.2:83197112-83197131 None:intergenic 30.0%
TAATAATATGTAGTGAGTGG+TGG - chr4.2:83198152-83198171 None:intergenic 30.0%
TACTCTATCAGAACCTTAAA+GGG + chr4.2:83196727-83196746 MS.gene99474:intron 30.0%
TCAGAATCATTATCATTCCT+CGG + chr4.2:83195061-83195080 MS.gene99474:CDS 30.0%
TCCTAATTGATATCATAAGC+TGG + chr4.2:83196963-83196982 MS.gene99474:intron 30.0%
TGCACAGATAAGTAAGAATT+GGG + chr4.2:83196573-83196592 MS.gene99474:intron 30.0%
TGTTATGATGGTTTACTTAG+TGG + chr4.2:83197173-83197192 MS.gene99474:intron 30.0%
TTCAATGCAATCATAAGTGT+CGG - chr4.2:83195621-83195640 None:intergenic 30.0%
TTCGAATGCTATACATATCT+TGG + chr4.2:83198980-83198999 MS.gene99474:intron 30.0%
TTCTTAGGCTGTTTATTAGT+GGG - chr4.2:83195310-83195329 None:intergenic 30.0%
TTGCACAGATAAGTAAGAAT+TGG + chr4.2:83196572-83196591 MS.gene99474:intron 30.0%
! CGAATCATTGTTCTAAATTG+TGG + chr4.2:83195952-83195971 MS.gene99474:intron 30.0%
! GTATTGCTGATGCTTTAAAT+GGG + chr4.2:83195362-83195381 MS.gene99474:CDS 30.0%
! TATTGCTGATGCTTTAAATG+GGG + chr4.2:83195363-83195382 MS.gene99474:CDS 30.0%
! TCAAAATTTACACTGACTAG+TGG + chr4.2:83199120-83199139 MS.gene99474:CDS 30.0%
! TGGAAGCATAAATAAAGATG+AGG - chr4.2:83197092-83197111 None:intergenic 30.0%
! TTCTGATTTGAGTCAATTAG+AGG + chr4.2:83195489-83195508 MS.gene99474:CDS 30.0%
!! AACTTTGTTGACTTCATGTT+TGG + chr4.2:83197927-83197946 MS.gene99474:CDS 30.0%
!! AATTAGTTTGTCAGTTAGAG+GGG - chr4.2:83196932-83196951 None:intergenic 30.0%
!! CAGTGTAAATTTTGATGTCA+AGG - chr4.2:83199115-83199134 None:intergenic 30.0%
!! GAATTAGTTTGTCAGTTAGA+GGG - chr4.2:83196933-83196952 None:intergenic 30.0%
!!! AGACGTAGTTTTGTGATTTT+TGG + chr4.2:83198823-83198842 MS.gene99474:intron 30.0%
!!! GACGTAGTTTTGTGATTTTT+GGG + chr4.2:83198824-83198843 MS.gene99474:intron 30.0%
!!! TTTGCTTTTTGAACTTGCAT+TGG + chr4.2:83196368-83196387 MS.gene99474:intron 30.0%
AAATCAAGAGGTAAGAATCC+TGG - chr4.2:83199059-83199078 None:intergenic 35.0%
AACAACATTTGTTTGCAGAC+AGG - chr4.2:83195145-83195164 None:intergenic 35.0%
AACGATTCACATGGATTCTT+TGG - chr4.2:83195172-83195191 None:intergenic 35.0%
AAGACAACTGTGTCTTTAGT+TGG - chr4.2:83198763-83198782 None:intergenic 35.0%
AAGTAAGGAGGTAAATAGAG+CGG + chr4.2:83196489-83196508 MS.gene99474:intron 35.0%
AAGTTGGGTAAAAGAGAAGA+TGG + chr4.2:83196753-83196772 MS.gene99474:intron 35.0%
AATTGCAACAAGGAGTAGAT+GGG - chr4.2:83196691-83196710 None:intergenic 35.0%
ACAACATTTGTTTGCAGACA+GGG - chr4.2:83195144-83195163 None:intergenic 35.0%
ACACTGTTATTGTTACCGTT+TGG + chr4.2:83197566-83197585 MS.gene99474:intron 35.0%
ACCATCCAAAACCTTTACAA+TGG - chr4.2:83197976-83197995 None:intergenic 35.0%
AGAACTATGTTGTGAATCGT+TGG + chr4.2:83196113-83196132 MS.gene99474:intron 35.0%
AGATGAGGTGAATGAAGATA+AGG - chr4.2:83197077-83197096 None:intergenic 35.0%
ATCTGTACATCAAAAGGACA+GGG - chr4.2:83197702-83197721 None:intergenic 35.0%
ATGTACATGAGGAATGTTGA+GGG - chr4.2:83198129-83198148 None:intergenic 35.0%
ATGTTTGTAGAGTAAGACCT+TGG + chr4.2:83195708-83195727 MS.gene99474:intron 35.0%
ATTTAAGGGCAATTGCAACA+AGG - chr4.2:83196701-83196720 None:intergenic 35.0%
CAAAGTGTGTTCAAACTAGT+AGG + chr4.2:83199190-83199209 MS.gene99474:CDS 35.0%
CAGAAACAGAATGTAGTAGT+AGG + chr4.2:83196538-83196557 MS.gene99474:intron 35.0%
CAGAATCTTGATTGTACTTG+TGG + chr4.2:83195280-83195299 MS.gene99474:CDS 35.0%
CCCAGCTTATGATATCAATT+AGG - chr4.2:83196967-83196986 None:intergenic 35.0%
CCTAAGAATTTGGATTCTGA+GGG + chr4.2:83195322-83195341 MS.gene99474:CDS 35.0%
CCTGTAAATTGCAAATCAAG+AGG - chr4.2:83199071-83199090 None:intergenic 35.0%
CTTCACTTCAAGATCAACAA+GGG - chr4.2:83198450-83198469 None:intergenic 35.0%
CTTGTTACGAAGAGCAATAT+TGG - chr4.2:83197270-83197289 None:intergenic 35.0%
GAACCTTAAAGGGAATAAGT+TGG + chr4.2:83196737-83196756 MS.gene99474:intron 35.0%
GAATAGAAAGGAAAGTAAGG+AGG + chr4.2:83196477-83196496 MS.gene99474:intron 35.0%
GAATGTAGTAGTAGGGAATT+AGG + chr4.2:83196546-83196565 MS.gene99474:intron 35.0%
GCTTCCTTACAATACCATTA+GGG + chr4.2:83197949-83197968 MS.gene99474:CDS 35.0%
GTACTCTATCAGAACCTTAA+AGG + chr4.2:83196726-83196745 MS.gene99474:intron 35.0%
GTAGAATTAAAGATAGAGGG+AGG + chr4.2:83198227-83198246 MS.gene99474:CDS 35.0%
GTTCAAGGACCTACTAATTT+GGG - chr4.2:83195867-83195886 None:intergenic 35.0%
TACCCAACTTATTCCCTTTA+AGG - chr4.2:83196743-83196762 None:intergenic 35.0%
TATCAGATGCGTATCAGTAT+CGG - chr4.2:83198645-83198664 None:intergenic 35.0%
TATTGCTCTTCGTAACAAGT+TGG + chr4.2:83197271-83197290 MS.gene99474:intron 35.0%
TCATATCATACTGTGTATCC+AGG + chr4.2:83199038-83199057 MS.gene99474:intron 35.0%
TCTCCTACACTATATAATGC+CGG - chr4.2:83198362-83198381 None:intergenic 35.0%
TTCAACATGACACAGATATG+AGG - chr4.2:83199017-83199036 None:intergenic 35.0%
TTCACTTCAAGATCAACAAG+GGG - chr4.2:83198449-83198468 None:intergenic 35.0%
TTCAGCATCTGTACATCAAA+AGG - chr4.2:83197708-83197727 None:intergenic 35.0%
TTCTTGCATAACGATTCACA+TGG - chr4.2:83195181-83195200 None:intergenic 35.0%
TTGTCTATGAAGATTCTGAG+AGG - chr4.2:83194905-83194924 None:intergenic 35.0%
! ACTAGTTTGAACACACTTTG+AGG - chr4.2:83199190-83199209 None:intergenic 35.0%
! AGTAAGAATTGGGAGAGTTT+TGG + chr4.2:83196583-83196602 MS.gene99474:intron 35.0%
! ATAGACTAGCCCACAAAAAA+TGG - chr4.2:83195794-83195813 None:intergenic 35.0%
! ATGCAGATGTTGATGGAATT+GGG + chr4.2:83195463-83195482 MS.gene99474:CDS 35.0%
! ATGTTTAGATATAGGTTGGG+TGG + chr4.2:83198869-83198888 MS.gene99474:intron 35.0%
! GAGGAATGATAATGATTCTG+AGG - chr4.2:83195062-83195081 None:intergenic 35.0%
! GGGTTTTTGTTAAGGACAAT+GGG + chr4.2:83195383-83195402 MS.gene99474:CDS 35.0%
! GGTATTGCTGATGCTTTAAA+TGG + chr4.2:83195361-83195380 MS.gene99474:CDS 35.0%
! GTAAGAATTGGGAGAGTTTT+GGG + chr4.2:83196584-83196603 MS.gene99474:intron 35.0%
! TAAGAATTGGGAGAGTTTTG+GGG + chr4.2:83196585-83196604 MS.gene99474:intron 35.0%
! TGAACATTGATGACACTGAA+TGG + chr4.2:83195209-83195228 MS.gene99474:CDS 35.0%
! TGTTTAGATATAGGTTGGGT+GGG + chr4.2:83198870-83198889 MS.gene99474:intron 35.0%
! TTGGACTTATGGAAAGGTAT+TGG - chr4.2:83198672-83198691 None:intergenic 35.0%
! TTGGATGGTAACTCTTTTGT+TGG + chr4.2:83197987-83198006 MS.gene99474:CDS 35.0%
!! AGTAGATGGGGAAAAGTATT+AGG - chr4.2:83196678-83196697 None:intergenic 35.0%
!! ATCGTTTTTGACTCTTCTTG+CGG - chr4.2:83194881-83194900 None:intergenic 35.0%
!! CCTCTAAAACCTTTATCTTG+CGG + chr4.2:83196062-83196081 MS.gene99474:intron 35.0%
!! CCTCTTGATTTGCAATTTAC+AGG + chr4.2:83199068-83199087 MS.gene99474:intron 35.0%
!! GGAATTAGTTTGTCAGTTAG+AGG - chr4.2:83196934-83196953 None:intergenic 35.0%
!! TAACGTCCATTTTAATGTCG+CGG - chr4.2:83197854-83197873 None:intergenic 35.0%
!! TGGACTTATGGAAAGGTATT+GGG - chr4.2:83198671-83198690 None:intergenic 35.0%
!! TTAATTTGTTGGGGGATCTT+GGG - chr4.2:83198310-83198329 None:intergenic 35.0%
!! TTTAATTTGTTGGGGGATCT+TGG - chr4.2:83198311-83198330 None:intergenic 35.0%
!!! GGACAATGGGTTTTTGTTTT+TGG + chr4.2:83195396-83195415 MS.gene99474:CDS 35.0%
!!! TCCATTGTAAAGGTTTTGGA+TGG + chr4.2:83197972-83197991 MS.gene99474:CDS 35.0%
AAGTATTAGGATCAACCCTC+TGG - chr4.2:83196665-83196684 None:intergenic 40.0%
ACAGGGTTCCAAACATCATT+TGG - chr4.2:83195127-83195146 None:intergenic 40.0%
ACCATTAGGGTCCATTGTAA+AGG + chr4.2:83197962-83197981 MS.gene99474:CDS 40.0%
ACCTTTACAATGGACCCTAA+TGG - chr4.2:83197966-83197985 None:intergenic 40.0%
AGACCGGCATTATATAGTGT+AGG + chr4.2:83198356-83198375 MS.gene99474:CDS 40.0%
AGAGAGAGTCATATACAAGC+GGG - chr4.2:83196839-83196858 None:intergenic 40.0%
AGCAAAAGCCTTGACTGAAA+TGG + chr4.2:83198283-83198302 MS.gene99474:CDS 40.0%
AGGCAAGAATTAGCAGAAAG+TGG - chr4.2:83198401-83198420 None:intergenic 40.0%
ATGGAAGTCACCAAAGAACT+TGG - chr4.2:83195445-83195464 None:intergenic 40.0%
ATTGCAACAAGGAGTAGATG+GGG - chr4.2:83196690-83196709 None:intergenic 40.0%
CAATTCCATCAACATCTGCA+TGG - chr4.2:83195464-83195483 None:intergenic 40.0%
CAATTGCAACAAGGAGTAGA+TGG - chr4.2:83196692-83196711 None:intergenic 40.0%
CAGAGAGAGTCATATACAAG+CGG - chr4.2:83196840-83196859 None:intergenic 40.0%
CATCTGTACATCAAAAGGAC+AGG - chr4.2:83197703-83197722 None:intergenic 40.0%
CATTGGAAAACAGGAGGTAA+TGG + chr4.2:83195525-83195544 MS.gene99474:intron 40.0%
CCCTCAGAATCCAAATTCTT+AGG - chr4.2:83195325-83195344 None:intergenic 40.0%
CCTTCACTTCAAGATCAACA+AGG - chr4.2:83198451-83198470 None:intergenic 40.0%
CCTTGTTGATCTTGAAGTGA+AGG + chr4.2:83198448-83198467 MS.gene99474:CDS 40.0%
CTAAATTGTGGTCACGATTG+TGG + chr4.2:83195964-83195983 MS.gene99474:intron 40.0%
GAAGATTGTGCCAAGTTCTT+TGG + chr4.2:83195432-83195451 MS.gene99474:CDS 40.0%
GAAGTCAACAAAGTTTCCAC+TGG - chr4.2:83197922-83197941 None:intergenic 40.0%
GAAGTGAAGGTGATATGCAT+TGG + chr4.2:83198461-83198480 MS.gene99474:CDS 40.0%
GATGTACATGAGGAATGTTG+AGG - chr4.2:83198130-83198149 None:intergenic 40.0%
GCCTAAGAATTTGGATTCTG+AGG + chr4.2:83195321-83195340 MS.gene99474:CDS 40.0%
GGCTTCCTTACAATACCATT+AGG + chr4.2:83197948-83197967 MS.gene99474:CDS 40.0%
GGTTCAAGGACCTACTAATT+TGG - chr4.2:83195868-83195887 None:intergenic 40.0%
TAAAGACACAGTTGTCTTCC+AGG + chr4.2:83198765-83198784 MS.gene99474:intron 40.0%
TAGTAAAATGAGGGTAACGC+AGG + chr4.2:83196010-83196029 MS.gene99474:intron 40.0%
TAGTAGCAAGTGGAAGAGAA+AGG - chr4.2:83194991-83195010 None:intergenic 40.0%
TCGATGCCAACAAATCTGAA+CGG + chr4.2:83196865-83196884 MS.gene99474:intron 40.0%
TCTGATCCTATTGATCCAGA+GGG + chr4.2:83196647-83196666 MS.gene99474:intron 40.0%
TGGACCCTAATGGTATTGTA+AGG - chr4.2:83197956-83197975 None:intergenic 40.0%
TGTACATGAGGAATGTTGAG+GGG - chr4.2:83198128-83198147 None:intergenic 40.0%
TTCAAGTGTTCAACACAAGC+AGG + chr4.2:83197046-83197065 MS.gene99474:intron 40.0%
TTGATCATCGTTGTTGTGTG+TGG - chr4.2:83195029-83195048 None:intergenic 40.0%
TTGTAGAGTAAGACCTTGGA+GGG + chr4.2:83195712-83195731 MS.gene99474:intron 40.0%
TTTGAATGTGAGACCTTGAG+AGG - chr4.2:83195760-83195779 None:intergenic 40.0%
TTTGTAGAGTAAGACCTTGG+AGG + chr4.2:83195711-83195730 MS.gene99474:intron 40.0%
! AAAACTCGGTATCTGGTTCA+AGG - chr4.2:83195882-83195901 None:intergenic 40.0%
! AATTTGGATTCTGAGGGTCA+GGG + chr4.2:83195328-83195347 MS.gene99474:CDS 40.0%
! AGGGTCCATTGTAAAGGTTT+TGG + chr4.2:83197968-83197987 MS.gene99474:CDS 40.0%
! ATTTACACTGACTAGTGGAC+TGG + chr4.2:83199125-83199144 MS.gene99474:CDS 40.0%
! CATGCAGATGTTGATGGAAT+TGG + chr4.2:83195462-83195481 MS.gene99474:CDS 40.0%
! CCAAACATCATTTGGAGTGA+GGG - chr4.2:83195119-83195138 None:intergenic 40.0%
! GGGGTTTTTGTTAAGGACAA+TGG + chr4.2:83195382-83195401 MS.gene99474:CDS 40.0%
! GTTTAGATATAGGTTGGGTG+GGG + chr4.2:83198871-83198890 MS.gene99474:intron 40.0%
! TCCAAACATCATTTGGAGTG+AGG - chr4.2:83195120-83195139 None:intergenic 40.0%
! TGCAGATGTTGATGGAATTG+GGG + chr4.2:83195464-83195483 MS.gene99474:CDS 40.0%
! TGGTTTGGACAATCAAAGTG+TGG - chr4.2:83198104-83198123 None:intergenic 40.0%
! TTTAGATATAGGTTGGGTGG+GGG + chr4.2:83198872-83198891 MS.gene99474:intron 40.0%
!! AACGTCCATTTTAATGTCGC+GGG - chr4.2:83197853-83197872 None:intergenic 40.0%
!! ACTTATGGAAAGGTATTGGG+GGG - chr4.2:83198668-83198687 None:intergenic 40.0%
!! CCGCAAGATAAAGGTTTTAG+AGG - chr4.2:83196065-83196084 None:intergenic 40.0%
!! GACTTATGGAAAGGTATTGG+GGG - chr4.2:83198669-83198688 None:intergenic 40.0%
!! GGACTTATGGAAAGGTATTG+GGG - chr4.2:83198670-83198689 None:intergenic 40.0%
!! GTGTGTGTGTGTTTTGGATA+GGG - chr4.2:83197534-83197553 None:intergenic 40.0%
!! TATACAGCTCTGGCTTTAGT+TGG + chr4.2:83195811-83195830 MS.gene99474:intron 40.0%
!! TGTGTGTGTGTGTTTTGGAT+AGG - chr4.2:83197535-83197554 None:intergenic 40.0%
!!! ATCGTTGGTCTTTTCAGTCT+AGG + chr4.2:83196128-83196147 MS.gene99474:intron 40.0%
!!! GTCCATGTGCCATTTTTTGT+GGG + chr4.2:83195782-83195801 MS.gene99474:intron 40.0%
!!! TCGTTGGTCTTTTCAGTCTA+GGG + chr4.2:83196129-83196148 MS.gene99474:intron 40.0%
ACGCAACATTGGAAAACAGG+AGG + chr4.2:83195519-83195538 MS.gene99474:CDS 45.0%
ACTGCTGATGCAAACTGGTT+TGG - chr4.2:83197813-83197832 None:intergenic 45.0%
AGCCCACAAAAAATGGCACA+TGG - chr4.2:83195787-83195806 None:intergenic 45.0%
AGGCGAATGATAGTAGCAAG+TGG - chr4.2:83195001-83195020 None:intergenic 45.0%
AGGTTAAGGTGTACTTGAGG+AGG - chr4.2:83197731-83197750 None:intergenic 45.0%
ATAATCTGTGCAGAAGCCAG+TGG + chr4.2:83197903-83197922 MS.gene99474:CDS 45.0%
ATTTGTCAGAGGCTCATGAG+AGG - chr4.2:83197751-83197770 None:intergenic 45.0%
CAATTGCAGCCGCAAGATAA+AGG - chr4.2:83196074-83196093 None:intergenic 45.0%
CACACGCAACATTGGAAAAC+AGG + chr4.2:83195516-83195535 MS.gene99474:CDS 45.0%
CCCTCACTCCAAATGATGTT+TGG + chr4.2:83195116-83195135 MS.gene99474:CDS 45.0%
CGTTGTTGTGTGTGGTTAAG+AGG - chr4.2:83195021-83195040 None:intergenic 45.0%
CTATGCTTCATAGACCACGT+GGG - chr4.2:83198582-83198601 None:intergenic 45.0%
CTCTGATCCTATTGATCCAG+AGG + chr4.2:83196646-83196665 MS.gene99474:intron 45.0%
CTCTGGATCAATAGGATCAG+AGG - chr4.2:83196648-83196667 None:intergenic 45.0%
CTTCAAGATCAACAAGGGGA+AGG - chr4.2:83198445-83198464 None:intergenic 45.0%
CTTTGACTGCTGATGCAAAC+TGG - chr4.2:83197818-83197837 None:intergenic 45.0%
GACACCAGGATTGAGTAACA+TGG - chr4.2:83198078-83198097 None:intergenic 45.0%
GACATGAGTCAGAAGCTCTT+AGG - chr4.2:83197391-83197410 None:intergenic 45.0%
GACTTCCATGCAGATGTTGA+TGG + chr4.2:83195456-83195475 MS.gene99474:CDS 45.0%
GAGACACCGTTCAGATTTGT+TGG - chr4.2:83196874-83196893 None:intergenic 45.0%
GAGAGGTTAAGGTGTACTTG+AGG - chr4.2:83197734-83197753 None:intergenic 45.0%
GATCAACCCTCTGGATCAAT+AGG - chr4.2:83196656-83196675 None:intergenic 45.0%
GATGGGATTTGTGAAGAGAC+CGG + chr4.2:83198340-83198359 MS.gene99474:CDS 45.0%
GCTACTCGAACAGTAATGTC+TGG + chr4.2:83196233-83196252 MS.gene99474:intron 45.0%
GCTACTCTGATGAGTGGTAA+AGG - chr4.2:83197415-83197434 None:intergenic 45.0%
GGAGTGATTTCTAAGTCGCA+TGG + chr4.2:83198248-83198267 MS.gene99474:CDS 45.0%
GGGTTTGTACGCTTTCATAG+AGG - chr4.2:83195099-83195118 None:intergenic 45.0%
TCATAAGCTGGGCGATCTAT+TGG + chr4.2:83196975-83196994 MS.gene99474:intron 45.0%
TCTATGCTTCATAGACCACG+TGG - chr4.2:83198583-83198602 None:intergenic 45.0%
TCTTGGGTCCATTTCAGTCA+AGG - chr4.2:83198294-83198313 None:intergenic 45.0%
TGTAGAGTAAGACCTTGGAG+GGG + chr4.2:83195713-83195732 MS.gene99474:intron 45.0%
TTGATGTCAAGGAAGCTGCA+AGG - chr4.2:83199104-83199123 None:intergenic 45.0%
TTGCACCCGCGACATTAAAA+TGG + chr4.2:83197845-83197864 MS.gene99474:CDS 45.0%
! AGAGGTTTTTGATGCTTCCG+AGG - chr4.2:83195081-83195100 None:intergenic 45.0%
! ATTTGGATTCTGAGGGTCAG+GGG + chr4.2:83195329-83195348 MS.gene99474:CDS 45.0%
! GAATTTGGATTCTGAGGGTC+AGG + chr4.2:83195327-83195346 MS.gene99474:CDS 45.0%
! GATATGCATTGGTGAAGCTG+AGG + chr4.2:83198472-83198491 MS.gene99474:CDS 45.0%
! GTACAAGAGTGTAGAGAGCT+TGG + chr4.2:83198025-83198044 MS.gene99474:CDS 45.0%
! GTGTGTGTGTGTGTGTGTTT+TGG - chr4.2:83197540-83197559 None:intergenic 45.0%
!! GGTTGGTCTCCCAAATTAGT+AGG + chr4.2:83195855-83195874 MS.gene99474:intron 45.0%
!!! CGTCCATGTGCCATTTTTTG+TGG + chr4.2:83195781-83195800 MS.gene99474:intron 45.0%
!! TAAAGAATTAATAAAATTAT+TGG - chr4.2:83198691-83198710 None:intergenic 5.0%
ATGTCAAGGAAGCTGCAAGG+AGG - chr4.2:83199101-83199120 None:intergenic 50.0%
CACCAGATACGACACTGACA+TGG - chr4.2:83195673-83195692 None:intergenic 50.0%
CAGAGGCTCATGAGAGGTTA+AGG - chr4.2:83197745-83197764 None:intergenic 50.0%
CGCAATGCTACTCTGATGAG+TGG - chr4.2:83197421-83197440 None:intergenic 50.0%
CGCTCCATGTTACTCAATCC+TGG + chr4.2:83198071-83198090 MS.gene99474:CDS 50.0%
GAGGAAGTCACACGCAACAT+TGG + chr4.2:83195508-83195527 MS.gene99474:CDS 50.0%
GTCCATGTCAGTGTCGTATC+TGG + chr4.2:83195668-83195687 MS.gene99474:intron 50.0%
TAGAGAGCTTGGACTCTTCG+TGG + chr4.2:83198036-83198055 MS.gene99474:CDS 50.0%
TGTCAGACACTGTCACGTGT+AGG - chr4.2:83195598-83195617 None:intergenic 50.0%
!! CTGATGCAAACTGGTTTGGC+GGG - chr4.2:83197809-83197828 None:intergenic 50.0%
!! GCTGATGCAAACTGGTTTGG+CGG - chr4.2:83197810-83197829 None:intergenic 50.0%
!! GTGAGTGGTGGATGTACATG+AGG - chr4.2:83198140-83198159 None:intergenic 50.0%
!! TGGCGGGTTGTCTTTGTTCT+TGG - chr4.2:83197793-83197812 None:intergenic 50.0%
!! TGGGCTAGTCTATACAGCTC+TGG + chr4.2:83195801-83195820 MS.gene99474:intron 50.0%
AGTAAGACCTTGGAGGGGCT+TGG + chr4.2:83195718-83195737 MS.gene99474:intron 55.0%
ATACAAGCGGGAGCCCTTGA+AGG - chr4.2:83196827-83196846 None:intergenic 55.0%
CATGAGGAATGTTGAGGGGC+TGG - chr4.2:83198124-83198143 None:intergenic 55.0%
CTTCATAGACCACGTGGGAG+GGG - chr4.2:83198577-83198596 None:intergenic 55.0%
CTTGGAGTGTGCTCCTCTCA+AGG + chr4.2:83195744-83195763 MS.gene99474:intron 55.0%
GCTTCATAGACCACGTGGGA+GGG - chr4.2:83198578-83198597 None:intergenic 55.0%
GTAAGACCTTGGAGGGGCTT+GGG + chr4.2:83195719-83195738 MS.gene99474:intron 55.0%
TCAAAGTGTGGTGCGACACC+AGG - chr4.2:83198092-83198111 None:intergenic 55.0%
TCTGAGGGTCAGGGGAATAG+TGG + chr4.2:83195337-83195356 MS.gene99474:CDS 55.0%
TGCTTCATAGACCACGTGGG+AGG - chr4.2:83198579-83198598 None:intergenic 55.0%
TTCATAGACCACGTGGGAGG+GGG - chr4.2:83198576-83198595 None:intergenic 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - chr4.2:83198119-83198138 None:intergenic 55.0%
AACCGCGCTGTCTACTCGTG+GGG - chr4.2:83195839-83195858 None:intergenic 60.0%
ACGTGGGAGGGGGATCAAAG+AGG - chr4.2:83198566-83198585 None:intergenic 60.0%
CAACCGCGCTGTCTACTCGT+GGG - chr4.2:83195840-83195859 None:intergenic 60.0%
CAAGATCCCAAGCCCCTCCA+AGG - chr4.2:83195728-83195747 None:intergenic 60.0%
CTTGGAGGGGCTTGGGATCT+TGG + chr4.2:83195726-83195745 MS.gene99474:intron 60.0%
GAGGGTCAGGGGAATAGTGG+TGG + chr4.2:83195340-83195359 MS.gene99474:CDS 60.0%
TCTTTGATCCCCCTCCCACG+TGG + chr4.2:83198565-83198584 MS.gene99474:intron 60.0%
TGCGGCTGCAATTGCTGCTG+CGG + chr4.2:83196080-83196099 MS.gene99474:intron 60.0%
AGCCCCACGAGTAGACAGCG+CGG + chr4.2:83195834-83195853 MS.gene99474:intron 65.0%
CCAACCGCGCTGTCTACTCG+TGG - chr4.2:83195841-83195860 None:intergenic 65.0%
CCACGAGTAGACAGCGCGGT+TGG + chr4.2:83195838-83195857 MS.gene99474:intron 65.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83194863 83199251 83194863 ID=MS.gene99474
chr4.2 mRNA 83194863 83199251 83194863 ID=MS.gene99474.t1;Parent=MS.gene99474
chr4.2 exon 83194863 83195540 83194863 ID=MS.gene99474.t1.exon1;Parent=MS.gene99474.t1
chr4.2 CDS 83194863 83195540 83194863 ID=cds.MS.gene99474.t1;Parent=MS.gene99474.t1
chr4.2 exon 83197720 83198493 83197720 ID=MS.gene99474.t1.exon2;Parent=MS.gene99474.t1
chr4.2 CDS 83197720 83198493 83197720 ID=cds.MS.gene99474.t1;Parent=MS.gene99474.t1
chr4.2 exon 83199090 83199251 83199090 ID=MS.gene99474.t1.exon3;Parent=MS.gene99474.t1
chr4.2 CDS 83199090 83199251 83199090 ID=cds.MS.gene99474.t1;Parent=MS.gene99474.t1
Gene Sequence

>MS.gene99474

ATGGCTTCTGAAGAACCGCAAGAAGAGTCAAAAACGATTCCTCTCAGAATCTTCATAGACAAAGAAAACAACAAAGTAGTCGCTGTAGAAGCAACAAAAGACTTCGTTGACACTCTCTTCAGCTTCCTTTCTCTTCCACTTGCTACTATCATTCGCCTCTTAACCACACACAACAACGATGATCAAAAACAAGAAGCCTCAGAATCATTATCATTCCTCGGAAGCATCAAAAACCTCTATGAAAGCGTACAAACCCTCACTCCAAATGATGTTTGGAACCCTGTCTGCAAACAAATGTTGTTGAATCCAAAGAATCCATGTGAATCGTTATGCAAGAAGCTTTTCTTGAACATTGATGACACTGAATGGTCAAGTAAGTTTTTCGTGTGTGATGCTTGTAACAAGTTTACAACTTTTCAGAATCTTGATTGTACTTGTGGAAAACCCACTAATAAACAGCCTAAGAATTTGGATTCTGAGGGTCAGGGGAATAGTGGTGGTATTGCTGATGCTTTAAATGGGGTTTTTGTTAAGGACAATGGGTTTTTGTTTTTGGTGTATGATGATTTGAAGATTGTGCCAAGTTCTTTGGTGACTTCCATGCAGATGTTGATGGAATTGGGGTATTCTGATTTGAGTCAATTAGAGGAAGTCACACGCAACATTGGAAAACAGGAGGTAATGGTGTTGTGTTTTGCAATGTGTATTTTTATGTCATGTTACTCTATTGCACCTACACGTGACAGTGTCTGACACCGACACTTATGATTGCATTGAATTATGTTATTTTCTTAAAATTTTACGTGTCCATGTCAGTGTCGTATCTGGTGTCCGTGTTTCATAACATGTTTGTAGAGTAAGACCTTGGAGGGGCTTGGGATCTTGGAGTGTGCTCCTCTCAAGGTCTCACATTCAAATCGTCCATGTGCCATTTTTTGTGGGCTAGTCTATACAGCTCTGGCTTTAGTTGGAGCCCCACGAGTAGACAGCGCGGTTGGTCTCCCAAATTAGTAGGTCCTTGAACCAGATACCGAGTTTTAAAAAAATAAATTGTAGCGTAAGAAAAATCCGAAAAAATATTTGAAACTGCGAATCATTGTTCTAAATTGTGGTCACGATTGTGGATACGATGCAAACCTTGTTATTGTAGTAAAATGAGGGTAACGCAGGCAATTGCAGTTGCGATGTCGTTGCAGAGACCTCTAAAACCTTTATCTTGCGGCTGCAATTGCTGCTGCGGACTACAATTTAGAACTATGTTGTGAATCGTTGGTCTTTTCAGTCTAGGGTTACTTAGAACTCAAGTCTTAAGTGTTTGCATTTTAGTCTAGTGTTAGTAGAATACACATGTTAACTATGAGTATCAAAATGCTACTCGAACAGTAATGTCTGGATCAAAATAACTAATGAATAATGGTTTTGAGATCTTTTCTGAGTTTTTCTTGTTTCACAGACAAGTTAACAATGTCCTATAATTTGAGGTATTGAATTGGTGATTCACTCTTTTTGCTTTTTGAACTTGCATTGGATGTTTAGACTGTTCTTGAATTATGAAACTTAACTTTATAACTTTCGTTTTTTAGGGTAGTTTGTATATGGAATATCATCTGTTAAGAATAGAAAGGAAAGTAAGGAGGTAAATAGAGCGGTCGTAACTGATATAGTTGTAAACTGACAGAAACAGAATGTAGTAGTAGGGAATTAGGGTGTTGCACAGATAAGTAAGAATTGGGAGAGTTTTGGGGCACTTTTGAATTAGCCTAGAGTTTATCACTTTCTATTCCTCTGATCCTATTGATCCAGAGGGTTGATCCTAATACTTTTCCCCATCTACTCCTTGTTGCAATTGCCCTTAAATCTATTGTACTCTATCAGAACCTTAAAGGGAATAAGTTGGGTAAAAGAGAAGATGGTTTTTACTGATTGAATAAAATTGAACAAAACCAAAGAAAAAGATTTAACCTTCAAGGGCTCCCGCTTGTATATGACTCTCTCTGTCTGATCGATGCCAACAAATCTGAACGGTGTCTCTGCCCTTACATCCCTTATTTAGTTATCGAACTAATCCCCTCTAACTGACAAACTAATTCCTCAAATTGTTCCTAATTGATATCATAAGCTGGGCGATCTATTGGTTAACAGTTCAACCATTTTTACACTAATTATTCTGATAAATATTGAATTTCAAGTGTTCAACACAAGCAGGTTGCACCTTATCTTCATTCACCTCATCTTTATTTATGCTTCCACATAAAGTTATTAGTGTACCATAAATGAAAAGGGAAAACATTACTTAATAAACATTCACAATGTTATGATGGTTTACTTAGTGGCTAGTTGTTGTACTTAAATTACGGACACACTATAAATCTTGAGTTAGAAATTAGTACTCCAAATATTCGTTCCAATATTGCTCTTCGTAACAAGTTGGCAAGTATTTTTTTTTAATGTAACAACTAACAAGTTTTTTTGTTTTTGCTGCTGTGTGATCAAGTAGAACATGCTGAAATTTGTTTGTCGTTGCACCTAAGAGCTTCTGACTCATGTCACCTTTACCACTCATCAGAGTAGCATTGCGTATCTCAACTAATTTGAGTGAAGAGTTAAAGGTGTTGAAATAGTTAAGAGAAAAATACTAACATAACAATTAATCAATATACTAATATTTCCCTATCCAAAACACACACACACACACACACACACACACTGTTATTGTTACCGTTTGGTTTAATATCTTGTATTTCTGTTATGTAATGTGAATTTGTTAGTGAAATATTTTAAAAGAATGTTATATTGGGGTCATTTGTATGTATGTTATCTTGGATAAAAATGTTTTCCCTGTCCTTTTGATGTACAGATGCTGAACCTCCTCAAGTACACCTTAACCTCTCATGAGCCTCTGACAAATACAATTTTGAAAAGCAGCTCCAAGAACAAAGACAACCCGCCAAACCAGTTTGCATCAGCAGTCAAAGTAAAAACTTGCACCCGCGACATTAAAATGGACGTTAAGCTAGTGCGAAGTAAATCTCAGAAAAAGATAATCTGTGCAGAAGCCAGTGGAAACTTTGTTGACTTCATGTTTGGCTTCCTTACAATACCATTAGGGTCCATTGTAAAGGTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGAGTGTAGAGAGCTTGGACTCTTCGTGGTGTACAAACTCGCGCTCCATGTTACTCAATCCTGGTGTCGCACCACACTTTGATTGTCCAAACCAGCCCCTCAACATTCCTCATGTACATCCACCACTCACTACATATTATTATGGTACAGGAACACCAAAGCAAGAGTATAATTATAGTTATTATGGTTATCAAACGGTAGAATTAAAGATAGAGGGAGGAGTGATTTCTAAGTCGCATGGATTGATTTATAAAGCAAAAGCCTTGACTGAAATGGACCCAAGATCCCCCAACAAATTAAAAAAAGATGTGATGGGATTTGTGAAGAGACCGGCATTATATAGTGTAGGAGATGATCTAAAAGTAAAACCACTTTCTGCTAATTCTTGCCTTTCATATTTGAAAGAATTAAACCTTCCCCTTGTTGATCTTGAAGTGAAGGTGATATGCATTGGTGAAGCTGAGGTAAAGTGACTACTATTACTAAATTTATAATTTTTTTTCCTTTTGTTTATTTGTTGTCTAAGTTTTTAGCCTCTTTGATCCCCCTCCCACGTGGTCTATGAAGCATAGATACTTCAAAATTAGATAAGTATTGATGTATGACACGTATCCGATACTGATACGCATCTGATACCCCCCAATACCTTTCCATAAGTCCAATAATTTTATTAATTCTTTATGAAAATATTTTCATTGATACTTCTCAAATACATCTTCAATACTTCTTACCAACTAAAGACACAGTTGTCTTCCAGGTACACTACATTTTTTATATGCTTTACTAACTAAAGAGACGTAGTTTTGTGATTTTTGGGTCACATTCTTTATAGAAATAATATGTTTAGATATAGGTTGGGTGGGGGAATTATATATGTAACTATGCTTACATGTTATGATTACAAGTACTTTTTTGTAAATTGCGATTACAATAATATCTTTATAAACGTTTTCGAATGCTATACATATCTTGGTTTTGTCGTATCCTCATATCTGTGTCATGTTGAATTCATATCATACTGTGTATCCAGGATTCTTACCTCTTGATTTGCAATTTACAGGCTTTGAGCCTCCTTGCAGCTTCCTTGACATCAAAATTTACACTGACTAGTGGACTGGAAGAATTTTTTAATGAGCAAAAACAAGATTCAAATTTGACCTCAAAGTGTGTTCAAACTAGTAGGCTTGATAAAGTTGCAAAGAAGCCTAAACTAGAAGATTGA
Protein sequence

>MS.gene99474.t1

MASEEPQEESKTIPLRIFIDKENNKVVAVEATKDFVDTLFSFLSLPLATIIRLLTTHNNDDQKQEASESLSFLGSIKNLYESVQTLTPNDVWNPVCKQMLLNPKNPCESLCKKLFLNIDDTEWSSKFFVCDACNKFTTFQNLDCTCGKPTNKQPKNLDSEGQGNSGGIADALNGVFVKDNGFLFLVYDDLKIVPSSLVTSMQMLMELGYSDLSQLEEVTRNIGKQEMLNLLKYTLTSHEPLTNTILKSSSKNKDNPPNQFASAVKVKTCTRDIKMDVKLVRSKSQKKIICAEASGNFVDFMFGFLTIPLGSIVKVLDGNSFVGCVDNLYKSVESLDSSWCTNSRSMLLNPGVAPHFDCPNQPLNIPHVHPPLTTYYYGTGTPKQEYNYSYYGYQTVELKIEGGVISKSHGLIYKAKALTEMDPRSPNKLKKDVMGFVKRPALYSVGDDLKVKPLSANSCLSYLKELNLPLVDLEVKVICIGEAEALSLLAASLTSKFTLTSGLEEFFNEQKQDSNLTSKCVQTSRLDKVAKKPKLED