AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89821

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89821.t1 MTR_7g063880 71.227 497 114 8 2 497 3 471 0.0 661
MS.gene89821.t1 MTR_7g063840 53.427 496 206 13 1 494 1 473 2.71e-155 451
MS.gene89821.t1 MTR_7g062280 50.196 510 219 10 1 494 1 491 6.40e-152 442
MS.gene89821.t1 MTR_7g063900 50.000 508 220 12 1 494 1 488 6.01e-150 437
MS.gene89821.t1 MTR_7g062300 51.313 495 210 13 1 487 1 472 5.54e-148 432
MS.gene89821.t1 MTR_7g063660 49.296 497 222 13 5 494 4 477 1.14e-143 421
MS.gene89821.t1 MTR_7g063660 31.633 98 60 2 252 343 3 99 7.13e-11 64.7
MS.gene89821.t1 MTR_7g063820 50.690 507 214 14 1 494 1 484 1.32e-140 413
MS.gene89821.t1 MTR_7g063920 48.800 500 183 10 2 494 3 436 9.87e-139 407
MS.gene89821.t1 MTR_7g063830 49.098 499 194 13 1 494 1 444 4.21e-137 403
MS.gene89821.t1 MTR_7g063890 49.012 506 225 11 2 494 19 504 1.33e-130 389
MS.gene89821.t1 MTR_3g007740 48.889 495 192 13 3 489 2 443 8.87e-129 382
MS.gene89821.t1 MTR_7g063670 48.491 497 194 13 8 494 13 457 3.51e-128 380
MS.gene89821.t1 MTR_7g066570 38.856 507 269 12 3 492 2 484 1.33e-97 306
MS.gene89821.t1 MTR_3g065200 41.667 504 216 19 11 497 4 446 8.09e-95 295
MS.gene89821.t1 MTR_7g066260 37.425 497 262 12 1 488 6 462 6.43e-93 291
MS.gene89821.t1 MTR_7g058710 37.209 516 269 15 1 494 1 483 3.61e-86 273
MS.gene89821.t1 MTR_7g063850 51.703 323 115 8 180 495 1 289 3.43e-85 265
MS.gene89821.t1 MTR_7g066410 34.345 527 296 11 1 490 1 514 7.91e-84 268
MS.gene89821.t1 MTR_7g012680 36.957 506 252 14 1 494 1 451 1.72e-82 263
MS.gene89821.t1 MTR_7g066350 36.765 476 243 14 1 461 1 433 5.06e-79 254
MS.gene89821.t1 MTR_7g066430 35.375 506 285 14 11 494 5 490 4.74e-78 253
MS.gene89821.t1 MTR_7g066340 35.141 498 268 13 1 488 1 453 6.08e-78 252
MS.gene89821.t1 MTR_7g066360 37.349 498 242 14 2 484 3 445 1.62e-74 243
MS.gene89821.t1 MTR_1g041315 34.375 480 252 14 6 461 11 451 1.24e-71 236
MS.gene89821.t1 MTR_7g066290 35.685 496 240 11 1 484 1 429 6.40e-70 229
MS.gene89821.t1 MTR_4g019310 34.280 528 281 21 3 495 2 498 1.84e-69 230
MS.gene89821.t1 MTR_7g066580 32.929 495 266 15 16 491 1 448 2.25e-68 226
MS.gene89821.t1 MTR_7g066580 29.046 241 134 11 11 229 216 441 3.87e-11 65.5
MS.gene89821.t1 MTR_7g066480 43.939 330 159 8 11 327 17 333 3.00e-67 223
MS.gene89821.t1 MTR_7g066400 34.375 512 241 19 1 490 1 439 1.10e-66 221
MS.gene89821.t1 MTR_7g066490 43.252 326 153 8 11 327 17 319 1.46e-64 215
MS.gene89821.t1 MTR_7g058700 31.984 494 218 10 22 494 531 927 2.65e-63 222
MS.gene89821.t1 MTR_2g029400 33.333 465 216 10 4 454 8 392 7.13e-62 207
MS.gene89821.t1 MTR_4g017410 31.818 528 285 22 3 494 2 490 3.62e-58 201
MS.gene89821.t1 MTR_7g066310 30.785 484 225 14 9 488 6 383 6.81e-57 194
MS.gene89821.t1 MTR_7g066310 29.148 223 122 7 6 227 190 377 5.71e-15 77.0
MS.gene89821.t1 MTR_7g062270 51.087 184 89 1 3 185 2 185 2.12e-56 192
MS.gene89821.t1 MTR_7g063690 44.014 284 91 7 2 283 3 220 1.90e-55 186
MS.gene89821.t1 MTR_7g063720 43.846 260 113 9 237 493 2 231 3.67e-55 185
MS.gene89821.t1 MTR_7g063720 28.571 217 133 7 12 227 25 220 1.18e-13 71.2
MS.gene89821.t1 MTR_4g017440 30.019 533 299 20 4 494 8 508 8.98e-53 186
MS.gene89821.t1 MTR_4g017830 29.755 531 312 21 1 494 1 507 9.89e-52 184
MS.gene89821.t1 MTR_4g017470 28.625 538 275 19 3 494 2 476 1.52e-47 172
MS.gene89821.t1 MTR_4g019340 29.980 497 282 20 1 460 29 496 8.26e-47 170
MS.gene89821.t1 MTR_1g014060 47.317 205 104 3 23 225 13 215 9.01e-46 164
MS.gene89821.t1 MTR_1g014060 29.167 216 117 9 284 494 206 390 2.44e-15 78.2
MS.gene89821.t1 MTR_4g017610 29.339 484 290 19 6 462 7 465 1.95e-43 160
MS.gene89821.t1 MTR_7g100420 35.562 329 178 11 161 484 94 393 6.61e-39 146
MS.gene89821.t1 MTR_7g100420 67.308 52 17 0 2 53 31 82 4.48e-14 74.3
MS.gene89821.t1 MTR_4g017550 28.226 496 264 25 37 484 18 469 7.22e-34 134
MS.gene89821.t1 MTR_4g017550 29.268 246 113 9 13 226 250 466 6.63e-14 74.3
MS.gene89821.t1 MTR_7g066280 27.674 430 241 14 89 488 1 390 1.46e-31 126
MS.gene89821.t1 MTR_7g066280 28.689 244 137 8 11 227 151 384 1.42e-14 75.9
MS.gene89821.t1 MTR_7g066680 30.094 319 168 9 205 484 32 334 4.27e-31 123
MS.gene89821.t1 MTR_8g023240 27.187 320 205 9 7 318 34 333 1.16e-26 114
MS.gene89821.t1 MTR_1g115440 32.685 257 131 8 1 233 1 239 5.92e-23 97.8
MS.gene89821.t1 MTR_8g445860 31.250 240 141 7 3 230 8 235 4.22e-21 92.8
MS.gene89821.t1 MTR_8g445860 24.186 215 141 7 254 454 9 215 9.83e-11 62.4
MS.gene89821.t1 MTR_0150s0010 32.520 246 139 8 1 230 1 235 2.35e-20 90.5
MS.gene89821.t1 MTR_7g063870 47.619 105 51 2 121 223 63 165 5.96e-19 87.0
MS.gene89821.t1 MTR_7g063870 66.667 57 19 0 4 60 25 81 3.14e-17 82.0
MS.gene89821.t1 MTR_2g067310 35.294 187 77 5 11 193 44 190 1.18e-18 85.9
MS.gene89821.t1 MTR_1g115455 28.400 250 139 9 1 230 1 230 8.48e-18 83.2
MS.gene89821.t1 MTR_7g063760 48.235 85 30 3 412 494 1 73 4.87e-17 76.3
MS.gene89821.t1 MTR_8g445920 43.590 78 39 1 1 78 1 73 1.45e-11 61.6
MS.gene89821.t1 MTR_8g044890 43.590 78 39 1 1 78 1 73 1.45e-11 61.6
MS.gene89821.t1 MTR_1g115450 37.615 109 60 3 3 110 2 103 4.06e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89821.t1 AT5G01120 30.315 508 288 18 11 484 10 485 9.36e-47 170
MS.gene89821.t1 AT3G09110 33.036 336 211 8 12 343 11 336 9.58e-46 163
MS.gene89821.t1 AT5G01130 29.703 505 289 16 1 494 1 450 1.95e-45 165
MS.gene89821.t1 AT5G01150 28.764 518 313 18 1 494 1 486 5.32e-45 165
MS.gene89821.t1 AT3G09140 29.851 536 289 19 1 490 1 495 1.29e-44 164
MS.gene89821.t1 AT5G01140 28.343 501 305 14 3 494 2 457 1.63e-42 157
MS.gene89821.t1 AT3G09140 28.369 564 289 20 1 490 1 523 2.46e-40 152
MS.gene89821.t1 AT3G09120 36.333 300 166 9 9 293 5 294 5.28e-40 147
MS.gene89821.t1 AT5G43240 28.655 513 294 18 11 484 10 489 2.64e-37 144
MS.gene89821.t1 AT5G43240 28.655 513 294 18 11 484 10 489 2.64e-37 144
MS.gene89821.t1 AT5G43240 28.655 513 294 18 11 484 10 489 2.64e-37 144
MS.gene89821.t1 AT5G37320 26.437 522 292 16 11 490 10 481 1.55e-31 127

Find 93 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGCTTCCTTGTTTCTCCTTT+GGG 0.184056 7.3:+41486201 MS.gene89821:CDS
AACGGTTGAGTAAATGTAAA+AGG 0.208138 7.3:-41486626 None:intergenic
AAGCTTCCTTGTTTCTCCTT+TGG 0.253692 7.3:+41486200 MS.gene89821:CDS
GGATCTACTAAAATGTTCTT+TGG 0.259006 7.3:+41485978 MS.gene89821:CDS
GCATAATAGTATAGCTGATT+TGG 0.270242 7.3:+41486275 MS.gene89821:CDS
CATGATTGTTGTTCCTCAAT+TGG 0.293295 7.3:+41486335 MS.gene89821:CDS
TAATAAGCTTCACAGTGATT+TGG 0.312794 7.3:-41486113 None:intergenic
TAAATAATTAAAACGTTCAT+TGG 0.341468 7.3:-41486481 None:intergenic
AAGGAGAAGTTTGTCACTAT+TGG 0.362524 7.3:+41486672 MS.gene89821:CDS
GAAGAATCTTGGCCCCTCTA+TGG 0.366680 7.3:-41486058 None:intergenic
TGAACCTCGAAAATACAATA+TGG 0.370857 7.3:+41486428 MS.gene89821:CDS
AACATCTGCAAAATCTTTCT+CGG 0.371262 7.3:-41485332 None:intergenic
TCCGTGACCACGTAAATTCT+TGG 0.379065 7.3:-41486570 None:intergenic
GGATCTCGATGAAGGGTGTC+TGG 0.384491 7.3:+41485468 MS.gene89821:CDS
AGCTTCTTGACACTGCCTTT+AGG 0.400104 7.3:+41485361 MS.gene89821:CDS
TTTGTTCACAAGAAGTTTCA+AGG 0.424038 7.3:-41485281 None:intergenic
GAAGCTTATTATAAGAAAAT+CGG 0.427086 7.3:+41486125 MS.gene89821:CDS
AGTAAATGTAAAAGGGTGAT+TGG 0.429390 7.3:-41486618 None:intergenic
CTGAATACATGAATCGTTTG+AGG 0.429843 7.3:+41485620 MS.gene89821:CDS
AATTGTCAGTTGGAAGATAT+CGG 0.436220 7.3:+41486388 MS.gene89821:CDS
AGCATGGGGCCATTCACAAT+TGG 0.452783 7.3:+41485415 MS.gene89821:CDS
TCAAAGGATTCCCGCACTCT+TGG 0.453341 7.3:-41485666 None:intergenic
AAATACAATATGGAAGGTAT+TGG 0.453537 7.3:+41486438 MS.gene89821:CDS
CGCTTAAATCAAAATATTGA+CGG 0.454596 7.3:-41486359 None:intergenic
GCAGTGTCTCAGCATATTGA+AGG 0.456934 7.3:+41486831 MS.gene89821:CDS
TTAATAGGAGGGAGTTCATT+GGG 0.461635 7.3:+41486237 MS.gene89821:CDS
TCACTTGCTAACTGAAGTTA+AGG 0.463987 7.3:+41486893 MS.gene89821:CDS
GGGCCAAGATTCTTCTCTTG+TGG 0.466673 7.3:+41486066 MS.gene89821:CDS
GGTGTCTGGGAACGTGGATA+AGG 0.470294 7.3:+41485482 MS.gene89821:CDS
TTTAATAGGAGGGAGTTCAT+TGG 0.477160 7.3:+41486236 MS.gene89821:CDS
AAACCACAAGAGAAGAATCT+TGG 0.480544 7.3:-41486069 None:intergenic
TTCAGCAAACAAGACTTTGT+TGG 0.490750 7.3:-41485308 None:intergenic
GCTTCCTTGTTTCTCCTTTG+GGG 0.491865 7.3:+41486202 MS.gene89821:CDS
TGTCACTGTCACTAAAGAAA+AGG 0.497602 7.3:+41485855 MS.gene89821:CDS
GGCTACGAACGCTTTGTCAT+GGG 0.504848 7.3:+41486546 MS.gene89821:CDS
AAATATTGACGGGCCAATTG+AGG 0.505994 7.3:-41486348 None:intergenic
CTTCCTTGTTTCTCCTTTGG+GGG 0.513430 7.3:+41486203 MS.gene89821:CDS
AACAAATTATTGTATGTGCA+AGG 0.513664 7.3:+41486150 MS.gene89821:CDS
TGGCTACGAACGCTTTGTCA+TGG 0.515142 7.3:+41486545 MS.gene89821:CDS
TTCCTTGTTTCTCCTTTGGG+GGG 0.515635 7.3:+41486204 MS.gene89821:CDS
TGCGTGGAGGATCTCGATGA+AGG 0.516149 7.3:+41485460 MS.gene89821:CDS
ATCATAACTGAACAGTTCAA+AGG 0.518489 7.3:-41485682 None:intergenic
CAAATTATTGTATGTGCAAG+GGG 0.524177 7.3:+41486152 MS.gene89821:CDS
TAATCTTCTGATGAGTTAGC+TGG 0.527130 7.3:-41485523 None:intergenic
AGACAGAAAACCATCCATAG+AGG 0.527169 7.3:+41486044 MS.gene89821:CDS
ACGGTTGAGTAAATGTAAAA+GGG 0.528785 7.3:-41486625 None:intergenic
GACAGAAAACCATCCATAGA+GGG 0.528903 7.3:+41486045 MS.gene89821:CDS
AGAAAAGTTCGTAATATCCA+TGG 0.533127 7.3:-41485782 None:intergenic
AAAATAACTGCCAAGAGTGC+GGG 0.537509 7.3:+41485656 MS.gene89821:CDS
GGAATTGTACGTTTAATAGG+AGG 0.539040 7.3:+41486225 MS.gene89821:CDS
AACTTCTGTTGACGATCTGA+AGG 0.539244 7.3:+41486653 MS.gene89821:CDS
TCAACATCTGCTCTATCAAA+CGG 0.543468 7.3:+41486864 MS.gene89821:CDS
TTCAGACAACCAATTGTGAA+TGG 0.545287 7.3:-41485424 None:intergenic
GACTGTCATGCAAAACTCCA+TGG 0.546370 7.3:+41485765 MS.gene89821:CDS
ACAAATTATTGTATGTGCAA+GGG 0.546473 7.3:+41486151 MS.gene89821:CDS
AAGGAGGAGATATGTGAATG+AGG 0.550943 7.3:+41486912 MS.gene89821:CDS
AATCTTGGCCCCTCTATGGA+TGG 0.554927 7.3:-41486054 None:intergenic
GATCTCGATGAAGGGTGTCT+GGG 0.555440 7.3:+41485469 MS.gene89821:CDS
GCTTAAATCAAAATATTGAC+GGG 0.557539 7.3:-41486358 None:intergenic
CAGTGTCTCAGCATATTGAA+GGG 0.557849 7.3:+41486832 MS.gene89821:CDS
TTACGTGGTCACGGATGACC+TGG 0.562305 7.3:+41486578 MS.gene89821:CDS
CGTCAACAGAAGTTTCAAAA+CGG 0.562417 7.3:-41486644 None:intergenic
CATCAATCACCTACACTGAC+TGG 0.563229 7.3:+41486525 MS.gene89821:CDS
AGTCTTGCAATAGTTCCTAA+AGG 0.563897 7.3:-41485376 None:intergenic
ATGAAGGGTGTCTGGGAACG+TGG 0.569556 7.3:+41485476 MS.gene89821:CDS
CTGCAATGGATTTGTGAGAA+GGG 0.573463 7.3:+41485714 MS.gene89821:CDS
CGATTCATGTATTCAGAGCA+AGG 0.583592 7.3:-41485613 None:intergenic
TCTGCAATGGATTTGTGAGA+AGG 0.584547 7.3:+41485713 MS.gene89821:CDS
CCTCGAAAATACAATATGGA+AGG 0.584924 7.3:+41486432 MS.gene89821:CDS
TGCTTCTACTCAAACAGAGG+AGG 0.591424 7.3:+41485252 MS.gene89821:CDS
ACCAAGAATTTACGTGGTCA+CGG 0.595998 7.3:+41486569 MS.gene89821:CDS
CTTGCTAACTGAAGTTAAGG+AGG 0.601679 7.3:+41486896 MS.gene89821:CDS
GCGTGGAGGATCTCGATGAA+GGG 0.602362 7.3:+41485461 MS.gene89821:CDS
AAAAGGGTGATTGGAGACAA+AGG 0.609586 7.3:-41486609 None:intergenic
GGCTGCTTCTACTCAAACAG+AGG 0.611746 7.3:+41485249 MS.gene89821:CDS
GAGACAAAGGTGAAATGACC+AGG 0.612367 7.3:-41486596 None:intergenic
CGTTCGTAGCCAGTCAGTGT+AGG 0.615213 7.3:-41486534 None:intergenic
GAATTGTACGTTTAATAGGA+GGG 0.615334 7.3:+41486226 MS.gene89821:CDS
GTGCATAAGAAGTACTCTGT+GGG 0.616518 7.3:-41485583 None:intergenic
GCTTCTACTCAAACAGAGGA+GGG 0.621158 7.3:+41485253 MS.gene89821:CDS
CATGGGACCAAGAATTTACG+TGG 0.621275 7.3:+41486563 MS.gene89821:CDS
TGCAATGGATTTGTGAGAAG+GGG 0.621803 7.3:+41485715 MS.gene89821:CDS
TCTGGGAACGTGGATAAGGA+AGG 0.621845 7.3:+41485486 MS.gene89821:CDS
GTAGAGAAAGAGACAAGCAT+GGG 0.622227 7.3:+41485400 MS.gene89821:CDS
TGTCACTATTGGCATCAAAG+AGG 0.622993 7.3:+41486683 MS.gene89821:CDS
GAACTCACTCTATCATTGCG+TGG 0.647546 7.3:+41485444 MS.gene89821:CDS
AGTGTCTCAGCATATTGAAG+GGG 0.654536 7.3:+41486833 MS.gene89821:CDS
TGTAGAGAAAGAGACAAGCA+TGG 0.657449 7.3:+41485399 MS.gene89821:CDS
GTATTCAGAGCAAGGAAACG+TGG 0.675034 7.3:-41485605 None:intergenic
ACAGAAAACCATCCATAGAG+GGG 0.692645 7.3:+41486046 MS.gene89821:CDS
GGTGCATAAGAAGTACTCTG+TGG 0.705012 7.3:-41485584 None:intergenic
CTCACTCTATCATTGCGTGG+AGG 0.709021 7.3:+41485447 MS.gene89821:CDS
TAGAGAAAGAGACAAGCATG+GGG 0.752295 7.3:+41485401 MS.gene89821:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAATAATTAAAACGTTCAT+TGG - chr7.3:41486484-41486503 None:intergenic 15.0%
!!! AATGAACGTTTTAATTATTT+AGG + chr7.3:41486483-41486502 MS.gene89821:CDS 15.0%
!! GAAGCTTATTATAAGAAAAT+CGG + chr7.3:41486125-41486144 MS.gene89821:CDS 20.0%
!!! TATTTCAATTTCTTGTTTTC+TGG + chr7.3:41485883-41485902 MS.gene89821:intron 20.0%
!!! TTAAATGACTTGTATTTTAC+AGG + chr7.3:41485951-41485970 MS.gene89821:intron 20.0%
! AACAAATTATTGTATGTGCA+AGG + chr7.3:41486150-41486169 MS.gene89821:CDS 25.0%
! ACAAATTATTGTATGTGCAA+GGG + chr7.3:41486151-41486170 MS.gene89821:CDS 25.0%
! ATGATGAAAAAATTCTGCAA+TGG + chr7.3:41485700-41485719 MS.gene89821:CDS 25.0%
! ATGTTTGGTTGTTTAGTAAT+AGG + chr7.3:41485910-41485929 MS.gene89821:intron 25.0%
! CGCTTAAATCAAAATATTGA+CGG - chr7.3:41486362-41486381 None:intergenic 25.0%
! GCTTAAATCAAAATATTGAC+GGG - chr7.3:41486361-41486380 None:intergenic 25.0%
!! AAATACAATATGGAAGGTAT+TGG + chr7.3:41486438-41486457 MS.gene89821:CDS 25.0%
!! TAAGAGGTAATATAGCTTTA+TGG - chr7.3:41486716-41486735 None:intergenic 25.0%
!!! TCAATATTTTGATTTAAGCG+AGG + chr7.3:41486362-41486381 MS.gene89821:CDS 25.0%
!!! TTGGCAGTTATTTTTGTTAA+AGG - chr7.3:41485650-41485669 None:intergenic 25.0%
AACATCTGCAAAATCTTTCT+CGG - chr7.3:41485335-41485354 None:intergenic 30.0%
AACGGTTGAGTAAATGTAAA+AGG - chr7.3:41486629-41486648 None:intergenic 30.0%
AATTGTCAGTTGGAAGATAT+CGG + chr7.3:41486388-41486407 MS.gene89821:CDS 30.0%
ACGGTTGAGTAAATGTAAAA+GGG - chr7.3:41486628-41486647 None:intergenic 30.0%
AGAAAAGTTCGTAATATCCA+TGG - chr7.3:41485785-41485804 None:intergenic 30.0%
AGTAAATGTAAAAGGGTGAT+TGG - chr7.3:41486621-41486640 None:intergenic 30.0%
ATCATAACTGAACAGTTCAA+AGG - chr7.3:41485685-41485704 None:intergenic 30.0%
CAAATTATTGTATGTGCAAG+GGG + chr7.3:41486152-41486171 MS.gene89821:CDS 30.0%
CACAAAAGCAGAAAAATAAG+AGG - chr7.3:41486732-41486751 None:intergenic 30.0%
GAATTGTACGTTTAATAGGA+GGG + chr7.3:41486226-41486245 MS.gene89821:CDS 30.0%
GCATAATAGTATAGCTGATT+TGG + chr7.3:41486275-41486294 MS.gene89821:CDS 30.0%
GGATCTACTAAAATGTTCTT+TGG + chr7.3:41485978-41485997 MS.gene89821:CDS 30.0%
GGTTGTTTAGTAATAGGTAT+TGG + chr7.3:41485916-41485935 MS.gene89821:intron 30.0%
TAATAAGCTTCACAGTGATT+TGG - chr7.3:41486116-41486135 None:intergenic 30.0%
TGAACCTCGAAAATACAATA+TGG + chr7.3:41486428-41486447 MS.gene89821:CDS 30.0%
TTTGTTCACAAGAAGTTTCA+AGG - chr7.3:41485284-41485303 None:intergenic 30.0%
! GACTTGTATTTTACAGGTAT+TGG + chr7.3:41485957-41485976 MS.gene89821:intron 30.0%
! GGGTGCAACTTTTATTATTA+AGG + chr7.3:41485735-41485754 MS.gene89821:CDS 30.0%
! GTTTAATGTTAGACTGACTT+AGG + chr7.3:41486765-41486784 MS.gene89821:intron 30.0%
!! TTTTGTTTCGAAAGAAGCAA+GGG + chr7.3:41486311-41486330 MS.gene89821:CDS 30.0%
!!! ACTGACAGATTTGTTTTTAG+GGG + chr7.3:41486014-41486033 MS.gene89821:CDS 30.0%
!!! CACTGACAGATTTGTTTTTA+GGG + chr7.3:41486013-41486032 MS.gene89821:CDS 30.0%
!!! TTGTTTTCTGGCTAAATGTT+TGG + chr7.3:41485895-41485914 MS.gene89821:intron 30.0%
AAAAATAACTGCCAAGAGTG+CGG + chr7.3:41485655-41485674 MS.gene89821:CDS 35.0%
AAACCACAAGAGAAGAATCT+TGG - chr7.3:41486072-41486091 None:intergenic 35.0%
AAGGAGAAGTTTGTCACTAT+TGG + chr7.3:41486672-41486691 MS.gene89821:CDS 35.0%
AGTCTTGCAATAGTTCCTAA+AGG - chr7.3:41485379-41485398 None:intergenic 35.0%
CCTAAAAACAAATCTGTCAG+TGG - chr7.3:41486015-41486034 None:intergenic 35.0%
CCTCGAAAATACAATATGGA+AGG + chr7.3:41486432-41486451 MS.gene89821:CDS 35.0%
CGTCAACAGAAGTTTCAAAA+CGG - chr7.3:41486647-41486666 None:intergenic 35.0%
CTGAATACATGAATCGTTTG+AGG + chr7.3:41485620-41485639 MS.gene89821:CDS 35.0%
GGAATTGTACGTTTAATAGG+AGG + chr7.3:41486225-41486244 MS.gene89821:CDS 35.0%
TAATCTTCTGATGAGTTAGC+TGG - chr7.3:41485526-41485545 None:intergenic 35.0%
TCAACATCTGCTCTATCAAA+CGG + chr7.3:41486864-41486883 MS.gene89821:CDS 35.0%
TGTCACTGTCACTAAAGAAA+AGG + chr7.3:41485855-41485874 MS.gene89821:CDS 35.0%
TTAATAGGAGGGAGTTCATT+GGG + chr7.3:41486237-41486256 MS.gene89821:CDS 35.0%
TTCAGACAACCAATTGTGAA+TGG - chr7.3:41485427-41485446 None:intergenic 35.0%
TTCAGCAAACAAGACTTTGT+TGG - chr7.3:41485311-41485330 None:intergenic 35.0%
TTTAATAGGAGGGAGTTCAT+TGG + chr7.3:41486236-41486255 MS.gene89821:CDS 35.0%
! AAAAAGTGCAAGTTCTGTGA+AGG + chr7.3:41485825-41485844 MS.gene89821:CDS 35.0%
! CATGATTGTTGTTCCTCAAT+TGG + chr7.3:41486335-41486354 MS.gene89821:CDS 35.0%
! CCTTCCATATTGTATTTTCG+AGG - chr7.3:41486435-41486454 None:intergenic 35.0%
! TCACTTGCTAACTGAAGTTA+AGG + chr7.3:41486893-41486912 MS.gene89821:CDS 35.0%
! TTTTCTCTGCTACAGAACTT+AGG + chr7.3:41485799-41485818 MS.gene89821:CDS 35.0%
!! CCACTGACAGATTTGTTTTT+AGG + chr7.3:41486012-41486031 MS.gene89821:CDS 35.0%
!!! GTTTTGTTTCGAAAGAAGCA+AGG + chr7.3:41486310-41486329 MS.gene89821:CDS 35.0%
AAAAGGGTGATTGGAGACAA+AGG - chr7.3:41486612-41486631 None:intergenic 40.0%
AACTTCTGTTGACGATCTGA+AGG + chr7.3:41486653-41486672 MS.gene89821:CDS 40.0%
ACAGAAAACCATCCATAGAG+GGG + chr7.3:41486046-41486065 MS.gene89821:CDS 40.0%
ACCAAGAATTTACGTGGTCA+CGG + chr7.3:41486569-41486588 MS.gene89821:CDS 40.0%
AGACAGAAAACCATCCATAG+AGG + chr7.3:41486044-41486063 MS.gene89821:CDS 40.0%
AGCGAGGAAAAATTGTCAGT+TGG + chr7.3:41486378-41486397 MS.gene89821:CDS 40.0%
AGTGTCTCAGCATATTGAAG+GGG + chr7.3:41486833-41486852 MS.gene89821:CDS 40.0%
CAGTGTCTCAGCATATTGAA+GGG + chr7.3:41486832-41486851 MS.gene89821:CDS 40.0%
CGATTCATGTATTCAGAGCA+AGG - chr7.3:41485616-41485635 None:intergenic 40.0%
CTTGCTAACTGAAGTTAAGG+AGG + chr7.3:41486896-41486915 MS.gene89821:CDS 40.0%
GACAGAAAACCATCCATAGA+GGG + chr7.3:41486045-41486064 MS.gene89821:CDS 40.0%
GGGGGAATTGTACGTTTAAT+AGG + chr7.3:41486222-41486241 MS.gene89821:CDS 40.0%
GTAGAGAAAGAGACAAGCAT+GGG + chr7.3:41485400-41485419 MS.gene89821:CDS 40.0%
TAGAGAAAGAGACAAGCATG+GGG + chr7.3:41485401-41485420 MS.gene89821:CDS 40.0%
TGTAGAGAAAGAGACAAGCA+TGG + chr7.3:41485399-41485418 MS.gene89821:CDS 40.0%
TGTCACTATTGGCATCAAAG+AGG + chr7.3:41486683-41486702 MS.gene89821:CDS 40.0%
! AAAATAACTGCCAAGAGTGC+GGG + chr7.3:41485656-41485675 MS.gene89821:CDS 40.0%
! AAATATTGACGGGCCAATTG+AGG - chr7.3:41486351-41486370 None:intergenic 40.0%
! AAGCTTCCTTGTTTCTCCTT+TGG + chr7.3:41486200-41486219 MS.gene89821:CDS 40.0%
! AGCTTCCTTGTTTCTCCTTT+GGG + chr7.3:41486201-41486220 MS.gene89821:CDS 40.0%
! CTGCAATGGATTTGTGAGAA+GGG + chr7.3:41485714-41485733 MS.gene89821:CDS 40.0%
! TCTGCAATGGATTTGTGAGA+AGG + chr7.3:41485713-41485732 MS.gene89821:CDS 40.0%
! TGCAATGGATTTGTGAGAAG+GGG + chr7.3:41485715-41485734 MS.gene89821:CDS 40.0%
!! GTGCATAAGAAGTACTCTGT+GGG - chr7.3:41485586-41485605 None:intergenic 40.0%
AAACGTACAATTCCCCCCAA+AGG - chr7.3:41486219-41486238 None:intergenic 45.0%
AGCTTCTTGACACTGCCTTT+AGG + chr7.3:41485361-41485380 MS.gene89821:CDS 45.0%
CATCAATCACCTACACTGAC+TGG + chr7.3:41486525-41486544 MS.gene89821:CDS 45.0%
CATGGGACCAAGAATTTACG+TGG + chr7.3:41486563-41486582 MS.gene89821:CDS 45.0%
GAACTCACTCTATCATTGCG+TGG + chr7.3:41485444-41485463 MS.gene89821:CDS 45.0%
GACTGTCATGCAAAACTCCA+TGG + chr7.3:41485765-41485784 MS.gene89821:CDS 45.0%
GAGACAAAGGTGAAATGACC+AGG - chr7.3:41486599-41486618 None:intergenic 45.0%
GCAGTGTCTCAGCATATTGA+AGG + chr7.3:41486831-41486850 MS.gene89821:CDS 45.0%
GCTTCTACTCAAACAGAGGA+GGG + chr7.3:41485253-41485272 MS.gene89821:CDS 45.0%
GTATTCAGAGCAAGGAAACG+TGG - chr7.3:41485608-41485627 None:intergenic 45.0%
TCCGTGACCACGTAAATTCT+TGG - chr7.3:41486573-41486592 None:intergenic 45.0%
TGCTTCTACTCAAACAGAGG+AGG + chr7.3:41485252-41485271 MS.gene89821:CDS 45.0%
! CTTCCTTGTTTCTCCTTTGG+GGG + chr7.3:41486203-41486222 MS.gene89821:CDS 45.0%
! GCTTCCTTGTTTCTCCTTTG+GGG + chr7.3:41486202-41486221 MS.gene89821:CDS 45.0%
! TTCCTTGTTTCTCCTTTGGG+GGG + chr7.3:41486204-41486223 MS.gene89821:CDS 45.0%
!! GGTGCATAAGAAGTACTCTG+TGG - chr7.3:41485587-41485606 None:intergenic 45.0%
AATCTTGGCCCCTCTATGGA+TGG - chr7.3:41486057-41486076 None:intergenic 50.0%
AGCATGGGGCCATTCACAAT+TGG + chr7.3:41485415-41485434 MS.gene89821:CDS 50.0%
CTCACTCTATCATTGCGTGG+AGG + chr7.3:41485447-41485466 MS.gene89821:CDS 50.0%
GAAGAATCTTGGCCCCTCTA+TGG - chr7.3:41486061-41486080 None:intergenic 50.0%
GGCTACGAACGCTTTGTCAT+GGG + chr7.3:41486546-41486565 MS.gene89821:CDS 50.0%
GGCTGCTTCTACTCAAACAG+AGG + chr7.3:41485249-41485268 MS.gene89821:CDS 50.0%
GGGCCAAGATTCTTCTCTTG+TGG + chr7.3:41486066-41486085 MS.gene89821:CDS 50.0%
TCAAAGGATTCCCGCACTCT+TGG - chr7.3:41485669-41485688 None:intergenic 50.0%
TCTGGGAACGTGGATAAGGA+AGG + chr7.3:41485486-41485505 MS.gene89821:CDS 50.0%
TGGCTACGAACGCTTTGTCA+TGG + chr7.3:41486545-41486564 MS.gene89821:CDS 50.0%
TTCCCCCCAAAGGAGAAACA+AGG - chr7.3:41486209-41486228 None:intergenic 50.0%
!! GATCTCGATGAAGGGTGTCT+GGG + chr7.3:41485469-41485488 MS.gene89821:CDS 50.0%
CGTTCGTAGCCAGTCAGTGT+AGG - chr7.3:41486537-41486556 None:intergenic 55.0%
GCGTGGAGGATCTCGATGAA+GGG + chr7.3:41485461-41485480 MS.gene89821:CDS 55.0%
GGTGTCTGGGAACGTGGATA+AGG + chr7.3:41485482-41485501 MS.gene89821:CDS 55.0%
TGCGTGGAGGATCTCGATGA+AGG + chr7.3:41485460-41485479 MS.gene89821:CDS 55.0%
TTACGTGGTCACGGATGACC+TGG + chr7.3:41486578-41486597 MS.gene89821:CDS 55.0%
!! ATGAAGGGTGTCTGGGAACG+TGG + chr7.3:41485476-41485495 MS.gene89821:CDS 55.0%
!! GGATCTCGATGAAGGGTGTC+TGG + chr7.3:41485468-41485487 MS.gene89821:CDS 55.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 41485247 41486932 41485247 ID=MS.gene89821
chr7.3 mRNA 41485247 41486932 41485247 ID=MS.gene89821.t1;Parent=MS.gene89821
chr7.3 exon 41485247 41485876 41485247 ID=MS.gene89821.t1.exon1;Parent=MS.gene89821.t1
chr7.3 CDS 41485247 41485876 41485247 ID=cds.MS.gene89821.t1;Parent=MS.gene89821.t1
chr7.3 exon 41485973 41486704 41485973 ID=MS.gene89821.t1.exon2;Parent=MS.gene89821.t1
chr7.3 CDS 41485973 41486704 41485973 ID=cds.MS.gene89821.t1;Parent=MS.gene89821.t1
chr7.3 exon 41486801 41486932 41486801 ID=MS.gene89821.t1.exon3;Parent=MS.gene89821.t1
chr7.3 CDS 41486801 41486932 41486801 ID=cds.MS.gene89821.t1;Parent=MS.gene89821.t1
Gene Sequence

>MS.gene89821

ATGGCTGCTTCTACTCAAACAGAGGAGGGAGTGACCTTGAAACTTCTTGTGAACAAAGACACCAACAAAGTCTTGTTTGCTGAAGCCGAGAAAGATTTTGCAGATGTTCTATTTAGCTTCTTGACACTGCCTTTAGGAACTATTGCAAGACTTGTAGAGAAAGAGACAAGCATGGGGCCATTCACAATTGGTTGTCTGAACTCACTCTATCATTGCGTGGAGGATCTCGATGAAGGGTGTCTGGGAACGTGGATAAGGAAGGAAATGCTGTTGCAGCCAGCTAACTCATCAGAAGATTATTGTAGCAGTATTAAACTTAATATTGATGACACTGATCCCACAGAGTACTTCTTATGCACCACGTTTCCTTGCTCTGAATACATGAATCGTTTGAGGATTTCCTTTAACAAAAATAACTGCCAAGAGTGCGGGAATCCTTTGAACTGTTCAGTTATGATGAAAAAATTCTGCAATGGATTTGTGAGAAGGGGTGCAACTTTTATTATTAAGGATGATTTGACTGTCATGCAAAACTCCATGGATATTACGAACTTTTCTCTGCTACAGAACTTAGGAATAAAAAGTGCAAGTTCTGTGAAGGAAATGACTGTCACTGTCACTAAAGAAAAGGTTTTATATTTCAATTTCTTGTTTTCTGGCTAAATGTTTGGTTGTTTAGTAATAGGTATTGGATCAATTCGTTTTTAAATGACTTGTATTTTACAGGTATTGGATCTACTAAAATGTTCTTTGGTATCAAAGACACCACTGACAGATTTGTTTTTAGGGGAAAATAAAGACAGAAAACCATCCATAGAGGGGCCAAGATTCTTCTCTTGTGGTTTTGAGAACAATAGTGATAGAATCCAAATCACTGTGAAGCTTATTATAAGAAAATCGGACAACAAATTATTGTATGTGCAAGGGGATTACGATTTTGCGAACAAACTTTTAAGCTTCCTTGTTTCTCCTTTGGGGGGAATTGTACGTTTAATAGGAGGGAGTTCATTGGGAAGCATTGATGCTTTGCATAATAGTATAGCTGATTTGGATAATTTTGAATGTTTTGTTTCGAAAGAAGCAAGGGACATGATTGTTGTTCCTCAATTGGCCCGTCAATATTTTGATTTAAGCGAGGAAAAATTGTCAGTTGGAAGATATCGGAGTTATTACTGTTATTATGAACCTCGAAAATACAATATGGAAGGTATTGGTCACAATCAAGTTTTCATTACCAATGAACGTTTTAATTATTTAGGAAATTGTTGTGAATTGTATCATCAATCACCTACACTGACTGGCTACGAACGCTTTGTCATGGGACCAAGAATTTACGTGGTCACGGATGACCTGGTCATTTCACCTTTGTCTCCAATCACCCTTTTACATTTACTCAACCGTTTTGAAACTTCTGTTGACGATCTGAAGGAGAAGTTTGTCACTATTGGCATCAAAGAGGTAATAGACCATAAAGCTATATTACCTCTTATTTTTCTGCTTTTGTGTTTGAACTTCGAAGTTTAATGTTAGACTGACTTAGGAAGTATTTTATAGCAAATGTTCACTTTAGCTTGTTTTCATTTTGCAGTGTCTCAGCATATTGAAGGGGCTTTAACATCAACATCTGCTCTATCAAACGGTCTTGCTCACTTGCTAACTGAAGTTAAGGAGGAGATATGTGAATGA
Protein sequence

>MS.gene89821.t1

MAASTQTEEGVTLKLLVNKDTNKVLFAEAEKDFADVLFSFLTLPLGTIARLVEKETSMGPFTIGCLNSLYHCVEDLDEGCLGTWIRKEMLLQPANSSEDYCSSIKLNIDDTDPTEYFLCTTFPCSEYMNRLRISFNKNNCQECGNPLNCSVMMKKFCNGFVRRGATFIIKDDLTVMQNSMDITNFSLLQNLGIKSASSVKEMTVTVTKEKVLDLLKCSLVSKTPLTDLFLGENKDRKPSIEGPRFFSCGFENNSDRIQITVKLIIRKSDNKLLYVQGDYDFANKLLSFLVSPLGGIVRLIGGSSLGSIDALHNSIADLDNFECFVSKEARDMIVVPQLARQYFDLSEEKLSVGRYRSYYCYYEPRKYNMEGIGHNQVFITNERFNYLGNCCELYHQSPTLTGYERFVMGPRIYVVTDDLVISPLSPITLLHLLNRFETSVDDLKEKFVTIGIKEQMFTLACFHFAVSQHIEGALTSTSALSNGLAHLLTEVKEEICE