AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50819

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50819.t1 MTR_4g017410 70.155 516 114 6 8 514 4 488 0.0 689
MS.gene50819.t1 MTR_4g017440 64.943 522 154 6 4 515 5 507 0.0 637
MS.gene50819.t1 MTR_4g017830 62.572 521 163 8 6 514 5 505 0.0 627
MS.gene50819.t1 MTR_4g017550 67.516 471 124 10 43 506 21 469 0.0 582
MS.gene50819.t1 MTR_4g017610 60.429 513 160 9 1 507 1 476 0.0 574
MS.gene50819.t1 MTR_4g017470 61.185 523 145 9 1 514 1 474 0.0 570
MS.gene50819.t1 MTR_4g019340 60.314 509 138 10 12 499 37 502 0.0 554
MS.gene50819.t1 MTR_4g019310 40.076 529 251 16 1 506 1 486 1.79e-106 333
MS.gene50819.t1 MTR_7g066570 34.070 543 274 19 1 516 1 486 1.99e-66 229
MS.gene50819.t1 MTR_7g066350 32.759 522 250 14 7 506 3 445 3.39e-65 223
MS.gene50819.t1 MTR_7g058710 31.903 536 272 13 1 506 1 473 2.37e-64 221
MS.gene50819.t1 MTR_7g063900 32.887 523 278 15 11 509 8 481 5.28e-59 207
MS.gene50819.t1 MTR_7g066340 36.170 376 204 11 1 357 1 359 1.61e-58 206
MS.gene50819.t1 MTR_7g066340 27.600 250 131 7 268 499 10 227 3.65e-15 79.0
MS.gene50819.t1 MTR_7g066340 26.609 233 130 5 6 238 256 447 2.59e-13 73.2
MS.gene50819.t1 MTR_7g063660 32.752 516 282 16 9 509 5 470 1.86e-58 205
MS.gene50819.t1 MTR_7g066430 31.818 550 289 16 14 533 5 498 2.21e-58 206
MS.gene50819.t1 MTR_7g066430 27.309 249 144 6 7 238 251 479 3.31e-14 76.3
MS.gene50819.t1 MTR_7g066410 32.216 537 281 16 14 506 11 508 1.83e-57 204
MS.gene50819.t1 MTR_7g066410 26.877 253 148 8 3 238 274 506 7.62e-11 65.5
MS.gene50819.t1 MTR_7g062280 32.537 544 272 18 1 509 1 484 2.66e-57 202
MS.gene50819.t1 MTR_7g063880 31.048 525 282 15 1 509 1 461 1.40e-56 200
MS.gene50819.t1 MTR_7g066260 31.569 529 250 19 9 506 11 458 9.59e-56 198
MS.gene50819.t1 MTR_7g012680 31.667 540 251 17 2 515 3 450 8.29e-55 195
MS.gene50819.t1 MTR_7g062300 32.150 535 274 19 1 509 1 472 8.66e-55 195
MS.gene50819.t1 MTR_7g066400 30.572 507 252 16 14 506 13 433 4.22e-52 187
MS.gene50819.t1 MTR_7g063890 31.643 493 267 15 14 484 28 472 5.09e-52 188
MS.gene50819.t1 MTR_7g063840 30.540 537 274 17 1 509 1 466 2.74e-51 186
MS.gene50819.t1 MTR_7g066580 29.961 514 267 14 19 511 1 442 1.38e-49 180
MS.gene50819.t1 MTR_1g041315 34.257 397 216 14 6 371 7 389 6.11e-49 179
MS.gene50819.t1 MTR_7g066290 35.695 367 200 11 9 355 11 361 8.80e-46 169
MS.gene50819.t1 MTR_7g063920 30.038 526 254 17 1 509 1 429 2.10e-45 168
MS.gene50819.t1 MTR_7g063830 29.207 517 255 14 14 509 11 437 2.67e-45 168
MS.gene50819.t1 MTR_7g066360 34.626 361 209 10 6 355 7 351 5.18e-45 168
MS.gene50819.t1 MTR_7g063820 31.589 535 282 20 1 509 1 477 9.52e-44 165
MS.gene50819.t1 MTR_7g066310 28.433 517 203 15 14 507 8 380 6.96e-43 160
MS.gene50819.t1 MTR_7g058700 33.795 361 152 11 23 347 529 838 2.05e-42 166
MS.gene50819.t1 MTR_3g007740 29.038 520 279 14 1 509 1 441 1.45e-41 157
MS.gene50819.t1 MTR_7g066480 36.176 340 167 12 14 333 17 326 3.37e-41 156
MS.gene50819.t1 MTR_4g019240 60.000 125 46 2 1 125 1 121 3.98e-40 145
MS.gene50819.t1 MTR_2g029400 28.543 501 240 13 9 498 10 403 5.25e-40 152
MS.gene50819.t1 MTR_3g065200 27.054 499 271 12 14 505 4 416 2.46e-39 151
MS.gene50819.t1 MTR_7g063670 28.077 520 281 15 6 509 8 450 3.03e-39 151
MS.gene50819.t1 MTR_7g066490 31.325 332 178 9 14 332 17 311 5.90e-32 129
MS.gene50819.t1 MTR_1g014060 33.482 224 123 8 22 236 9 215 9.91e-24 104
MS.gene50819.t1 MTR_7g066280 30.252 238 131 6 102 331 1 211 2.11e-23 103
MS.gene50819.t1 MTR_7g066280 27.308 260 144 8 6 238 143 384 8.36e-15 77.4
MS.gene50819.t1 MTR_7g066680 29.643 280 158 7 221 490 37 287 7.28e-21 95.1
MS.gene50819.t1 MTR_7g063720 29.675 246 118 5 273 509 26 225 2.64e-20 91.3
MS.gene50819.t1 MTR_7g062270 35.714 196 100 9 8 194 5 183 3.98e-20 93.6
MS.gene50819.t1 MTR_1g115440 30.041 243 137 9 14 241 12 236 2.21e-18 85.5
MS.gene50819.t1 MTR_1g115440 26.190 252 142 6 269 504 10 233 3.52e-15 76.3
MS.gene50819.t1 MTR_8g445860 28.253 269 112 9 269 504 12 232 5.34e-18 84.3
MS.gene50819.t1 MTR_8g445860 29.876 241 143 8 11 241 11 235 1.53e-16 80.1
MS.gene50819.t1 MTR_7g100420 28.857 350 183 14 173 506 94 393 3.25e-16 82.0
MS.gene50819.t1 MTR_7g100420 58.000 50 21 0 13 62 39 88 3.07e-11 66.2
MS.gene50819.t1 MTR_0150s0010 28.390 236 142 6 14 237 11 231 5.91e-16 78.6
MS.gene50819.t1 MTR_0150s0010 28.685 251 145 7 265 504 5 232 8.09e-15 75.1
MS.gene50819.t1 MTR_6g006800 72.917 48 11 1 1 48 1 46 8.29e-15 70.1
MS.gene50819.t1 MTR_1g115450 42.500 80 38 1 269 340 6 85 1.82e-13 68.6
MS.gene50819.t1 MTR_1g115455 39.080 87 45 1 262 340 1 87 7.66e-13 69.3
MS.gene50819.t1 MTR_1g115455 26.971 241 143 6 14 241 10 230 8.26e-13 68.9
MS.gene50819.t1 MTR_8g445920 41.791 67 39 0 265 331 5 71 2.33e-12 64.3
MS.gene50819.t1 MTR_8g044890 41.791 67 39 0 265 331 5 71 2.33e-12 64.3
MS.gene50819.t1 MTR_0150s0030 38.372 86 43 2 269 345 7 91 1.18e-11 65.1
MS.gene50819.t1 MTR_4g019330 47.500 80 40 2 437 514 107 186 2.21e-11 63.9
MS.gene50819.t1 MTR_1g078040 40.777 206 79 13 517 700 31 215 2.40e-11 67.8
MS.gene50819.t1 MTR_1g115470 41.250 80 39 1 269 340 5 84 3.26e-11 66.6
MS.gene50819.t1 MTR_8g445910 39.535 86 42 2 269 345 7 91 3.90e-11 64.7
MS.gene50819.t1 MTR_8g044880 39.506 81 40 1 269 340 7 87 7.80e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50819.t1 AT5G01130 30.664 512 275 15 7 509 3 443 3.66e-57 201
MS.gene50819.t1 AT5G01120 30.427 539 310 18 10 525 6 502 9.75e-54 193
MS.gene50819.t1 AT5G43240 30.930 527 285 18 13 506 9 489 4.03e-50 183
MS.gene50819.t1 AT5G43240 30.930 527 285 18 13 506 9 489 4.03e-50 183
MS.gene50819.t1 AT5G43240 30.930 527 285 18 13 506 9 489 4.03e-50 183
MS.gene50819.t1 AT5G01140 30.303 528 262 20 16 517 12 459 4.02e-49 179
MS.gene50819.t1 AT3G09140 28.913 543 287 18 10 509 6 492 3.40e-48 177
MS.gene50819.t1 AT5G01150 29.709 515 293 16 16 509 12 478 1.55e-47 176
MS.gene50819.t1 AT3G09110 34.535 333 189 10 10 332 6 319 9.41e-45 164
MS.gene50819.t1 AT3G09140 27.496 571 287 19 10 509 6 520 3.77e-43 164
MS.gene50819.t1 AT3G09120 33.234 337 177 12 10 324 3 313 5.66e-41 152
MS.gene50819.t1 AT3G09120 27.419 248 143 6 270 504 6 229 8.06e-16 79.7
MS.gene50819.t1 AT5G37320 27.767 515 293 17 20 509 18 478 3.27e-37 146
MS.gene50819.t1 AT5G43240 29.107 347 193 10 179 506 33 345 3.08e-24 105

Find 174 sgRNAs with CRISPR-Local

Find 245 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAAACTTTGGTCCATTT+TGG 0.099278 4.1:-80029271 None:intergenic
GCCACCTTTGGCTCGCTATT+TGG 0.177964 4.1:+80031246 MS.gene50819:CDS
AAACCAACAGCCTCGAAATT+TGG 0.181777 4.1:+80029211 MS.gene50819:CDS
GCCTCTCACAAATACAATTT+TGG 0.184722 4.1:+80030070 MS.gene50819:CDS
GGACCTCCTTCCGTCAATTT+TGG 0.186758 4.1:+80031177 MS.gene50819:CDS
CCTTGGTTCCAGTGCAATTA+AGG 0.221659 4.1:-80030627 None:intergenic
AGGATCCATTGTAAAGCTTT+TGG 0.229184 4.1:+80030283 MS.gene50819:CDS
CTTGCAGCTGTTGATGGAAT+TGG 0.234153 4.1:+80029337 MS.gene50819:CDS
TTCTTTACTCGGCGTGATAT+CGG 0.236275 4.1:+80031206 MS.gene50819:CDS
GAACACCCAGCTTATTATTA+TGG 0.239352 4.1:+80030443 MS.gene50819:CDS
CCAAAATGGACCAAAGTTTC+TGG 0.249363 4.1:+80029271 MS.gene50819:CDS
TGTTGACCCCTCTTTCTCTT+TGG 0.261951 4.1:-80030476 None:intergenic
GCAGCTTCTATACCGTCTTT+TGG 0.269844 4.1:+80031468 MS.gene50819:CDS
ACTACTCCTACGAATTCTTT+TGG 0.270872 4.1:+80031282 MS.gene50819:CDS
TTGATCATTGTTGCTGTTGA+TGG 0.292170 4.1:-80028922 None:intergenic
GCAAAGTAAATCTCAGAAAA+AGG 0.300596 4.1:+80030196 MS.gene50819:CDS
AAAGGTGGCGGAACCGAAAA+TGG 0.307297 4.1:-80031232 None:intergenic
TCATCATCCACCTCCGCATT+TGG 0.313927 4.1:+80031078 MS.gene50819:CDS
TCTTCTGGTACACCGTCTTT+TGG 0.322220 4.1:+80031438 MS.gene50819:CDS
TCGGCTTCAAATACAGCTTC+AGG 0.323250 4.1:+80031312 MS.gene50819:CDS
AATATTGCGGGTGCCCAAAA+TGG 0.336180 4.1:+80029257 MS.gene50819:CDS
GACTTACTTGCAGCTGTTGA+TGG 0.338778 4.1:+80029331 MS.gene50819:CDS
CAGTGATTCACATGGATTCC+TGG 0.341087 4.1:-80029069 None:intergenic
CCGTTTGGAGTACCGTCCTT+TGG 0.341591 4.1:+80031369 MS.gene50819:CDS
GGATTGAGTAACACAGAAAC+AGG 0.345671 4.1:-80030365 None:intergenic
ACCTACACTCAGACGATGTT+TGG 0.345816 4.1:+80029012 MS.gene50819:CDS
TCAGATTTCGCGGATTATAA+TGG 0.349431 4.1:+80030503 MS.gene50819:CDS
TGTTTCCTTACCATGCCATT+AGG 0.356298 4.1:+80030263 MS.gene50819:CDS
AAGGCTTCTACACCATTGTT+TGG 0.357666 4.1:+80031528 MS.gene50819:CDS
TTGCGCCGTCGTTTGGAGTT+TGG 0.360350 4.1:+80031565 MS.gene50819:CDS
GGAGTACCATCATCATCTTC+TGG 0.362322 4.1:+80031423 MS.gene50819:CDS
TTGCAGCTGTTGATGGAATT+GGG 0.365599 4.1:+80029338 MS.gene50819:CDS
TCGGCTTCTACACCATTGTT+TGG 0.369254 4.1:+80031402 MS.gene50819:CDS
GTGATAGAAGAAAAGATAGA+AGG 0.378763 4.1:+80030560 MS.gene50819:CDS
TTTCGAGGCTGTTGGTTTGT+TGG 0.381093 4.1:-80029206 None:intergenic
ACGGCTTCTACACCGTCGTT+TGG 0.385815 4.1:+80031498 MS.gene50819:CDS
TGCTGTTGATGGTGGCTAAA+AGG 0.392005 4.1:-80028911 None:intergenic
GTGTAGGCTTTGAACTTCCT+TGG 0.395429 4.1:+80030910 MS.gene50819:intron
CATCGTCTGAGTGTAGGTTT+TGG 0.399061 4.1:-80029007 None:intergenic
ACAGCTTCAGGGTCGTCGTT+TGG 0.405842 4.1:+80031324 MS.gene50819:CDS
GTTTCTGTGTTACTCAATCC+TGG 0.427223 4.1:+80030368 MS.gene50819:CDS
TTTGAATGTTCGATTGCAAA+AGG 0.429014 4.1:-80031007 None:intergenic
TGCGAAGTCCTTAATTGCAC+TGG 0.430913 4.1:+80030619 MS.gene50819:CDS
CAAGAGAATAGTAATATTGC+GGG 0.432706 4.1:+80029245 MS.gene50819:CDS
CAAAACCTTCATTGTACTTG+TGG 0.434397 4.1:+80029179 MS.gene50819:CDS
ATTTGTGAGAGGCTCATTAG+AGG 0.437734 4.1:-80030060 None:intergenic
AAAGAATTCGTAGGAGTAGT+AGG 0.438431 4.1:-80031279 None:intergenic
CCGGCATCTTCAACTCCGTT+TGG 0.439075 4.1:+80031354 MS.gene50819:CDS
CCTGGTATAGCACCACAGTT+TGG 0.441678 4.1:+80030386 MS.gene50819:CDS
GAGAGGCTCATTAGAGGTTA+AGG 0.444319 4.1:-80030054 None:intergenic
AGGAGAAGAAGTTGCGGCAG+AGG 0.454329 4.1:-80031115 None:intergenic
CATAAGTAAAATCAATGTTA+AGG 0.456872 4.1:+80030169 MS.gene50819:CDS
AACAAATGTTGCTGCATCCC+AGG 0.457734 4.1:+80029051 MS.gene50819:CDS
CCTGTACCATAATAATAAGC+TGG 0.458318 4.1:-80030449 None:intergenic
AACGACGGCGCAAAAGCCGC+TGG 0.458595 4.1:-80031555 None:intergenic
TTTGACCTGTTGGGAGACCT+TGG 0.460627 4.1:-80030644 None:intergenic
ACTGTTGATGCAGATTGCTT+AGG 0.463635 4.1:-80030122 None:intergenic
TTCAGACGCGACTCAATTAG+AGG 0.464556 4.1:+80029364 MS.gene50819:CDS
TCATCAGACACCAGAAACTT+TGG 0.465061 4.1:-80029281 None:intergenic
TCCTCAATTTCAAAACCAAA+AGG 0.467135 4.1:+80031035 MS.gene50819:CDS
TTTGGATCCTCTTCTTTACT+CGG 0.473778 4.1:+80031195 MS.gene50819:CDS
GGAAGGAGATGGCAAGAAAG+AGG 0.475700 4.1:-80031145 None:intergenic
CCAGCTTATTATTATGGTAC+AGG 0.485999 4.1:+80030449 MS.gene50819:CDS
GGAATGTTGAGTGGTTGGTT+TGG 0.486563 4.1:-80030413 None:intergenic
TTGTTGTTGTTGAGAAGCCA+TGG 0.486870 4.1:-80028751 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4.1:-80028881 None:intergenic
TAATGGTAATCGTATAATGA+TGG 0.489399 4.1:+80030520 MS.gene50819:CDS
TGGGATTTGTGAAGAGAGAA+GGG 0.491787 4.1:+80030678 MS.gene50819:CDS
AGCTTTACAATGGATCCTAA+TGG 0.496722 4.1:-80030278 None:intergenic
TGGAACCAAGGTCTCCCAAC+AGG 0.497799 4.1:+80030639 MS.gene50819:CDS
ACCTTTGGCTCGCTATTTGG+TGG 0.499467 4.1:+80031249 MS.gene50819:CDS
CCAAACTGTGGTGCTATACC+AGG 0.500141 4.1:-80030386 None:intergenic
ATTAACCTTGACTAGTGGAC+TGG 0.503661 4.1:+80030951 MS.gene50819:CDS
AGCTTCCTATCTCTTCCACT+TGG 0.503728 4.1:+80028876 MS.gene50819:CDS
TTGAATGTTCGATTGCAAAA+GGG 0.510409 4.1:-80031006 None:intergenic
CACACACAACATTGGCAAGC+AGG 0.511161 4.1:+80029391 MS.gene50819:CDS
GAGAGTGTCAACAAAATCCT+TGG 0.515103 4.1:-80028850 None:intergenic
GCCAAAATTGTATTTGTGAG+AGG 0.523537 4.1:-80030071 None:intergenic
CCAAAGGACGGTACTCCAAA+CGG 0.525259 4.1:-80031369 None:intergenic
AGAGAAGGGTTATATTGTGT+AGG 0.527009 4.1:+80030692 MS.gene50819:CDS
CGGCTTCAAATACAGCTTCA+GGG 0.527253 4.1:+80031313 MS.gene50819:CDS
GGCAAGAAAGAGGAGGCAAA+AGG 0.531197 4.1:-80031135 None:intergenic
GTCGTCGTTTGGAGCATCAC+CGG 0.531624 4.1:+80031335 MS.gene50819:CDS
TGAATGTTCGATTGCAAAAG+GGG 0.532394 4.1:-80031005 None:intergenic
CCATCTCCTTCCTCTTCTAG+AGG 0.534956 4.1:+80031156 MS.gene50819:CDS
CCTTAATTGCACTGGAACCA+AGG 0.535146 4.1:+80030627 MS.gene50819:CDS
ACGACGGCGCAAAAGCCGCT+GGG 0.536665 4.1:-80031554 None:intergenic
GTAACACAGAAACAGGGTTA+AGG 0.537772 4.1:-80030358 None:intergenic
AGTGATTCACATGGATTCCT+GGG 0.538053 4.1:-80029068 None:intergenic
AATCCAAATTTCGAGGCTGT+TGG 0.539114 4.1:-80029214 None:intergenic
ATCATTGTTGCTGTTGATGG+TGG 0.539275 4.1:-80028919 None:intergenic
CAGGAACACCAAAGAGAAAG+AGG 0.539842 4.1:+80030468 MS.gene50819:CDS
TCTGAGGAATGTTGAGTGGT+TGG 0.545273 4.1:-80030418 None:intergenic
AGCCGATGACGATGCTCCAA+AGG 0.545629 4.1:-80031385 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.547627 4.1:+80030331 MS.gene50819:CDS
AAGGAATAGTGATGATTCTG+AGG 0.547834 4.1:-80028955 None:intergenic
CTGTACCATAATAATAAGCT+GGG 0.548563 4.1:-80030448 None:intergenic
TGGATCCTAATGGCATGGTA+AGG 0.551509 4.1:-80030268 None:intergenic
TCAACATTCCTCAGATTTCG+CGG 0.551509 4.1:+80030493 MS.gene50819:CDS
ATAGAAGAAAAGATAGAAGG+AGG 0.551511 4.1:+80030563 MS.gene50819:CDS
GTCCTTTGGAGCATCGTCAT+CGG 0.554103 4.1:+80031383 MS.gene50819:CDS
AATGCGGAGGTGGATGATGA+AGG 0.554362 4.1:-80031075 None:intergenic
AGATGCGAATGAACAGAAAG+TGG 0.554908 4.1:-80030734 None:intergenic
TTACTTATGTTACCGGTGCA+AGG 0.557753 4.1:-80030155 None:intergenic
TGAGGATCCAAATGCGGAGG+TGG 0.558954 4.1:-80031085 None:intergenic
TTTCAAGATCATCAAGAGGA+AGG 0.560065 4.1:-80030778 None:intergenic
AAATAGCGAGCCAAAGGTGG+CGG 0.560413 4.1:-80031244 None:intergenic
GTGGGATTTGTGAAGAGAGA+AGG 0.565305 4.1:+80030677 MS.gene50819:CDS
CCGTACGAATCCAAATTTCG+AGG 0.565407 4.1:-80029221 None:intergenic
GCCGTTAGAGCTCCAAAAGA+CGG 0.565551 4.1:-80031480 None:intergenic
CGGAGGTGGATGATGAAGGA+GGG 0.572769 4.1:-80031071 None:intergenic
TCAAGAGAATAGTAATATTG+CGG 0.576099 4.1:+80029244 MS.gene50819:CDS
TTCATTTCAAGATCATCAAG+AGG 0.577676 4.1:-80030782 None:intergenic
AGAAGAGGATCCAAAATTGA+CGG 0.578525 4.1:-80031187 None:intergenic
TCAAAATTAACCTTGACTAG+TGG 0.580631 4.1:+80030946 MS.gene50819:CDS
CCTCGAAATTTGGATTCGTA+CGG 0.581423 4.1:+80029221 MS.gene50819:CDS
GCGGAGGTGGATGATGAAGG+AGG 0.581895 4.1:-80031072 None:intergenic
TACAATGGATCCTAATGGCA+TGG 0.582946 4.1:-80030273 None:intergenic
GATAAGCATTGGTGAAGTTG+AGG 0.584497 4.1:+80030808 MS.gene50819:CDS
AGATCCCAAAAGCTTTACAA+TGG 0.585059 4.1:-80030288 None:intergenic
ACCAAATAGCGAGCCAAAGG+TGG 0.585195 4.1:-80031247 None:intergenic
GATCCAAAATTGACGGAAGG+AGG 0.589093 4.1:-80031180 None:intergenic
TGTTGTTGTTGAGAAGCCAT+GGG 0.593299 4.1:-80028750 None:intergenic
ACCACCAAATAGCGAGCCAA+AGG 0.596309 4.1:-80031250 None:intergenic
GTCTCCCAGTCCACTAGTCA+AGG 0.597674 4.1:-80030956 None:intergenic
GCCTTTGATGTTCCAAACGA+CGG 0.598640 4.1:-80031510 None:intergenic
GGCAAAAGGAGAAGAAGTTG+CGG 0.601085 4.1:-80031121 None:intergenic
AGGAATAGTGATGATTCTGA+GGG 0.601982 4.1:-80028954 None:intergenic
ATTATAATCCGCGAAATCTG+AGG 0.603102 4.1:-80030501 None:intergenic
CCTCTAGAAGAGGAAGGAGA+TGG 0.603237 4.1:-80031156 None:intergenic
CGACGGCGCAAAAGCCGCTG+GGG 0.604534 4.1:-80031553 None:intergenic
GCTGCCGGTGCTCCAAAAGA+CGG 0.605110 4.1:-80031450 None:intergenic
TTGATGCAGATTGCTTAGGT+AGG 0.607887 4.1:-80030118 None:intergenic
GAAGTCAACAAAATCACCGT+TGG 0.609986 4.1:-80030234 None:intergenic
CCAAACGGAGTTGAAGATGC+CGG 0.610712 4.1:-80031354 None:intergenic
GTTAGATTAATGCCTTGCAC+CGG 0.610804 4.1:+80030143 MS.gene50819:CDS
GGAAGGAGGTCCTCTAGAAG+AGG 0.613771 4.1:-80031166 None:intergenic
GGAGGTCCTCTAGAAGAGGA+AGG 0.615174 4.1:-80031162 None:intergenic
CGTATAATGATGGATTGTAG+TGG 0.615640 4.1:+80030530 MS.gene50819:CDS
TGCAGCTGTTGATGGAATTG+GGG 0.616569 4.1:+80029339 MS.gene50819:CDS
ACCGTCGTTTGGAACATCAA+AGG 0.616749 4.1:+80031509 MS.gene50819:CDS
TAAGCTGGGTGTTCAATCTG+AGG 0.617424 4.1:-80030434 None:intergenic
AAGTAAGTCACTGACGAACA+TGG 0.618986 4.1:-80029317 None:intergenic
TTAACCTTGACTAGTGGACT+GGG 0.619919 4.1:+80030952 MS.gene50819:CDS
GAAATGAAGCTGATAAGCAT+TGG 0.620330 4.1:+80030797 MS.gene50819:CDS
AGGAACACCAAAGAGAAAGA+GGG 0.620352 4.1:+80030469 MS.gene50819:CDS
GAGGAAGTCACACACAACAT+TGG 0.621785 4.1:+80029383 MS.gene50819:CDS
TGCGGCAGAGGTTGTCTGTG+AGG 0.627436 4.1:-80031103 None:intergenic
GAGGATCCAAAATTGACGGA+AGG 0.631203 4.1:-80031183 None:intergenic
AAAGACGGTATAGAAGCTGC+CGG 0.631499 4.1:-80031465 None:intergenic
GTGATATATGCAGAAGCCAA+CGG 0.632346 4.1:+80030218 MS.gene50819:CDS
TATCACGCCGAGTAAAGAAG+AGG 0.639003 4.1:-80031202 None:intergenic
TCCAAACATCGTCTGAGTGT+AGG 0.640477 4.1:-80029013 None:intergenic
TTGATGTCAAGAATGCTCCA+AGG 0.651294 4.1:-80030927 None:intergenic
GCCGCTGGGGATCCAAACAA+TGG 0.651558 4.1:-80031540 None:intergenic
AGGAGATGGCAAGAAAGAGG+AGG 0.653093 4.1:-80031142 None:intergenic
GGTGTACCAGAAGATGATGA+TGG 0.659132 4.1:-80031429 None:intergenic
GTTGCCCCAAAAGAATTCGT+AGG 0.667496 4.1:-80031288 None:intergenic
GGTGGCTAAAAGGCGGACAA+TGG 0.668124 4.1:-80028901 None:intergenic
ACCATTGTTTGGATCCCCAG+CGG 0.670336 4.1:+80031539 MS.gene50819:CDS
TCAATCTGAGGAATGTTGAG+TGG 0.670562 4.1:-80030422 None:intergenic
GGAACACCAAAGAGAAAGAG+GGG 0.676448 4.1:+80030470 MS.gene50819:CDS
TTCATGCACAGTGATTCACA+TGG 0.677672 4.1:-80029077 None:intergenic
ACACAACATTGGCAAGCAGG+AGG 0.678135 4.1:+80029394 MS.gene50819:CDS
GATGACGATGCTCCAAAGGA+CGG 0.678623 4.1:-80031381 None:intergenic
AGGCGGACAATGGTGCCAAG+TGG 0.686421 4.1:-80028891 None:intergenic
CAAAACCAAACTCCAAACGA+CGG 0.692161 4.1:-80031570 None:intergenic
CTGTGAGGATCCAAATGCGG+AGG 0.699470 4.1:-80031088 None:intergenic
TAATTTGTACAAGAGTGTGG+AGG 0.710790 4.1:+80030334 MS.gene50819:CDS
GATGATGGTACTCCAAACAA+TGG 0.720142 4.1:-80031414 None:intergenic
TGGTTTGGACAACCAAACTG+TGG 0.720651 4.1:-80030398 None:intergenic
TGTCTGTGAGGATCCAAATG+CGG 0.722055 4.1:-80031091 None:intergenic
GATTGAGTAACACAGAAACA+GGG 0.732239 4.1:-80030364 None:intergenic
TGTTGATGGTGGCTAAAAGG+CGG 0.769976 4.1:-80028908 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTAAGATCTATTTAATTTT+CGG - chr4.1:80029743-80029762 None:intergenic 10.0%
!!! TCTAAATTTTACCATAATAA+AGG - chr4.1:80029577-80029596 None:intergenic 15.0%
!!! TTAAGATCTATTTAATTTTC+GGG - chr4.1:80029742-80029761 None:intergenic 15.0%
!! CATAAGTAAAATCAATGTTA+AGG + chr4.1:80030169-80030188 MS.gene50819:CDS 20.0%
!! TAATTAATATTCACAATCTC+AGG + chr4.1:80029823-80029842 MS.gene50819:intron 20.0%
!! TTTATTGAGAACCTTTATTA+TGG + chr4.1:80029563-80029582 MS.gene50819:intron 20.0%
!!! ATTGATTTTACTTATGTTAC+CGG - chr4.1:80030165-80030184 None:intergenic 20.0%
!!! TCACAAATATATGATTTTAG+AGG - chr4.1:80029641-80029660 None:intergenic 20.0%
! TAATGGTAATCGTATAATGA+TGG + chr4.1:80030520-80030539 MS.gene50819:CDS 25.0%
! TCAAGAGAATAGTAATATTG+CGG + chr4.1:80029244-80029263 MS.gene50819:CDS 25.0%
! TCAATATCTGTACATCAAAA+AGG - chr4.1:80030019-80030038 None:intergenic 25.0%
!! TTTGTCTACTACGATTTTAA+GGG - chr4.1:80028799-80028818 None:intergenic 25.0%
!!! AAGCAACAAAGTTATTTTTC+TGG + chr4.1:80028821-80028840 MS.gene50819:CDS 25.0%
!!! ACCTTTTTCGAACAAAAAAA+AGG - chr4.1:80029985-80030004 None:intergenic 25.0%
!!! TCCTTTTTTTTGTTCGAAAA+AGG + chr4.1:80029981-80030000 MS.gene50819:intron 25.0%
AACCACAAATTAAAACCATG+AGG - chr4.1:80029540-80029559 None:intergenic 30.0%
ACAAGTAAAGGAAATCAAAG+AGG - chr4.1:80030889-80030908 None:intergenic 30.0%
AGGTAATTAATTCTGTTGTG+TGG + chr4.1:80029414-80029433 MS.gene50819:intron 30.0%
ATAGAAGAAAAGATAGAAGG+AGG + chr4.1:80030563-80030582 MS.gene50819:CDS 30.0%
CAAGAGAATAGTAATATTGC+GGG + chr4.1:80029245-80029264 MS.gene50819:CDS 30.0%
CTGTACCATAATAATAAGCT+GGG - chr4.1:80030451-80030470 None:intergenic 30.0%
GCAAAGTAAATCTCAGAAAA+AGG + chr4.1:80030196-80030215 MS.gene50819:CDS 30.0%
GGAAGTCAATTAGTTAGATA+AGG + chr4.1:80029906-80029925 MS.gene50819:intron 30.0%
GTGATAGAAGAAAAGATAGA+AGG + chr4.1:80030560-80030579 MS.gene50819:CDS 30.0%
TATATTTGTGACTGCAATTG+AGG + chr4.1:80029650-80029669 MS.gene50819:intron 30.0%
TCAAAATTAACCTTGACTAG+TGG + chr4.1:80030946-80030965 MS.gene50819:CDS 30.0%
TCCTCAATTTCAAAACCAAA+AGG + chr4.1:80031035-80031054 MS.gene50819:CDS 30.0%
TGAAGATTCGTGATTAAGAT+TGG - chr4.1:80029517-80029536 None:intergenic 30.0%
TTCATTTCAAGATCATCAAG+AGG - chr4.1:80030785-80030804 None:intergenic 30.0%
TTGAATGTTCGATTGCAAAA+GGG - chr4.1:80031009-80031028 None:intergenic 30.0%
TTGTACCAAGTTTAAAGGTT+CGG + chr4.1:80029787-80029806 MS.gene50819:intron 30.0%
TTTGAATGTTCGATTGCAAA+AGG - chr4.1:80031010-80031029 None:intergenic 30.0%
! AGAGGTTTTTGATGTTTTCA+AGG - chr4.1:80028977-80028996 None:intergenic 30.0%
! CACACAAAAAACTTGTTTGA+TGG - chr4.1:80029131-80029150 None:intergenic 30.0%
! GTTTGTCTACTACGATTTTA+AGG - chr4.1:80028800-80028819 None:intergenic 30.0%
! TCTTTGATTTCCTTTACTTG+TGG + chr4.1:80030888-80030907 MS.gene50819:intron 30.0%
! TTGTCTACTACGATTTTAAG+GGG - chr4.1:80028798-80028817 None:intergenic 30.0%
!!! TCCTTTTGGTTTTGAAATTG+AGG - chr4.1:80031039-80031058 None:intergenic 30.0%
!!! TTGGTTTTGAAATTGAGGAA+GGG - chr4.1:80031034-80031053 None:intergenic 30.0%
!!! TTTGGTTTTGAAATTGAGGA+AGG - chr4.1:80031035-80031054 None:intergenic 30.0%
AAAGAATTCGTAGGAGTAGT+AGG - chr4.1:80031282-80031301 None:intergenic 35.0%
AAGAGTAGAATAGTGCAGTT+AGG + chr4.1:80029884-80029903 MS.gene50819:intron 35.0%
AAGGAATAGTGATGATTCTG+AGG - chr4.1:80028958-80028977 None:intergenic 35.0%
AGAAGAGGATCCAAAATTGA+CGG - chr4.1:80031190-80031209 None:intergenic 35.0%
AGAGAAGGGTTATATTGTGT+AGG + chr4.1:80030692-80030711 MS.gene50819:CDS 35.0%
AGAGTAGAATAGTGCAGTTA+GGG + chr4.1:80029885-80029904 MS.gene50819:intron 35.0%
AGATCCCAAAAGCTTTACAA+TGG - chr4.1:80030291-80030310 None:intergenic 35.0%
AGCTTTACAATGGATCCTAA+TGG - chr4.1:80030281-80030300 None:intergenic 35.0%
AGGAATAGTGATGATTCTGA+GGG - chr4.1:80028957-80028976 None:intergenic 35.0%
AGTACTACTACATTTCCTGA+AGG - chr4.1:80029686-80029705 None:intergenic 35.0%
ATTATAATCCGCGAAATCTG+AGG - chr4.1:80030504-80030523 None:intergenic 35.0%
ATTTCCTTTACTTGTGGTGT+AGG + chr4.1:80030894-80030913 MS.gene50819:intron 35.0%
CAAAACCTTCATTGTACTTG+TGG + chr4.1:80029179-80029198 MS.gene50819:CDS 35.0%
CAGGAAATGTAGTAGTACTA+GGG + chr4.1:80029687-80029706 MS.gene50819:intron 35.0%
CCAGCTTATTATTATGGTAC+AGG + chr4.1:80030449-80030468 MS.gene50819:CDS 35.0%
CCTGTACCATAATAATAAGC+TGG - chr4.1:80030452-80030471 None:intergenic 35.0%
GAAATGAAGCTGATAAGCAT+TGG + chr4.1:80030797-80030816 MS.gene50819:CDS 35.0%
GAACACCCAGCTTATTATTA+TGG + chr4.1:80030443-80030462 MS.gene50819:CDS 35.0%
GATTGAGTAACACAGAAACA+GGG - chr4.1:80030367-80030386 None:intergenic 35.0%
GCCAAAATTGTATTTGTGAG+AGG - chr4.1:80030074-80030093 None:intergenic 35.0%
GTACTACTACATTTCCTGAA+GGG - chr4.1:80029685-80029704 None:intergenic 35.0%
TCAGGAAATGTAGTAGTACT+AGG + chr4.1:80029686-80029705 MS.gene50819:intron 35.0%
TGAATGTTCGATTGCAAAAG+GGG - chr4.1:80031008-80031027 None:intergenic 35.0%
TTTCAAGATCATCAAGAGGA+AGG - chr4.1:80030781-80030800 None:intergenic 35.0%
TTTGGATCCTCTTCTTTACT+CGG + chr4.1:80031195-80031214 MS.gene50819:CDS 35.0%
! ACTACTCCTACGAATTCTTT+TGG + chr4.1:80031282-80031301 MS.gene50819:CDS 35.0%
! AGATTTTAGGCAGTTTCGAA+AGG - chr4.1:80030981-80031000 None:intergenic 35.0%
! AGGATCCATTGTAAAGCTTT+TGG + chr4.1:80030283-80030302 MS.gene50819:CDS 35.0%
! AGGTCAAAAATAGGTGTTGT+GGG + chr4.1:80030659-80030678 MS.gene50819:CDS 35.0%
! CAGAAACTTTGGTCCATTTT+GGG - chr4.1:80029273-80029292 None:intergenic 35.0%
! CTACTCCTACGAATTCTTTT+GGG + chr4.1:80031283-80031302 MS.gene50819:CDS 35.0%
! GCCTCTCACAAATACAATTT+TGG + chr4.1:80030070-80030089 MS.gene50819:CDS 35.0%
! GGATCCATTGTAAAGCTTTT+GGG + chr4.1:80030284-80030303 MS.gene50819:CDS 35.0%
! GTAGCTTGTACCAAGTTTAA+AGG + chr4.1:80029782-80029801 MS.gene50819:intron 35.0%
! GTTTTCCACAAGTACAATGA+AGG - chr4.1:80029187-80029206 None:intergenic 35.0%
! TAATTTGTACAAGAGTGTGG+AGG + chr4.1:80030334-80030353 MS.gene50819:CDS 35.0%
! TACTCCTACGAATTCTTTTG+GGG + chr4.1:80031284-80031303 MS.gene50819:CDS 35.0%
! TCAGATTTCGCGGATTATAA+TGG + chr4.1:80030503-80030522 MS.gene50819:CDS 35.0%
! TGGTAATTTGTACAAGAGTG+TGG + chr4.1:80030331-80030350 MS.gene50819:CDS 35.0%
! TTGATCATTGTTGCTGTTGA+TGG - chr4.1:80028925-80028944 None:intergenic 35.0%
!! CAAAAGGGGTTTGAGATTTT+AGG - chr4.1:80030994-80031013 None:intergenic 35.0%
!! CGTATAATGATGGATTGTAG+TGG + chr4.1:80030530-80030549 MS.gene50819:CDS 35.0%
!! TACTACGATTTTAAGGGGAA+TGG - chr4.1:80028793-80028812 None:intergenic 35.0%
!! TATTTTTCTGGAAGCAACCA+AGG + chr4.1:80028833-80028852 MS.gene50819:CDS 35.0%
!!! AACACCTATTTTTGACCTGT+TGG - chr4.1:80030657-80030676 None:intergenic 35.0%
!!! ACACCTATTTTTGACCTGTT+GGG - chr4.1:80030656-80030675 None:intergenic 35.0%
!!! AGGTAGGCTGTCTTTATTTT+TGG - chr4.1:80030105-80030124 None:intergenic 35.0%
!!! CACCTCATGGTTTTAATTTG+TGG + chr4.1:80029535-80029554 MS.gene50819:intron 35.0%
!!! GGTGTGAAGTTTTTTCCTTT+TGG - chr4.1:80031053-80031072 None:intergenic 35.0%
AAACCAACAGCCTCGAAATT+TGG + chr4.1:80029211-80029230 MS.gene50819:CDS 40.0%
AAAGCCTACACCACAAGTAA+AGG - chr4.1:80030901-80030920 None:intergenic 40.0%
AAGGCTTCTACACCATTGTT+TGG + chr4.1:80031528-80031547 MS.gene50819:CDS 40.0%
AAGTAAGTCACTGACGAACA+TGG - chr4.1:80029320-80029339 None:intergenic 40.0%
AATCACGAATCTTCACCTCA+TGG + chr4.1:80029522-80029541 MS.gene50819:intron 40.0%
AATCCAAATTTCGAGGCTGT+TGG - chr4.1:80029217-80029236 None:intergenic 40.0%
ACTGTTGATGCAGATTGCTT+AGG - chr4.1:80030125-80030144 None:intergenic 40.0%
AGATGCGAATGAACAGAAAG+TGG - chr4.1:80030737-80030756 None:intergenic 40.0%
AGGAACACCAAAGAGAAAGA+GGG + chr4.1:80030469-80030488 MS.gene50819:CDS 40.0%
AGTGATTCACATGGATTCCT+GGG - chr4.1:80029071-80029090 None:intergenic 40.0%
ATGGTGTATCAGTTAGTGTC+GGG + chr4.1:80029714-80029733 MS.gene50819:intron 40.0%
ATTTGTGAGAGGCTCATTAG+AGG - chr4.1:80030063-80030082 None:intergenic 40.0%
CAAAACCAAACTCCAAACGA+CGG - chr4.1:80031573-80031592 None:intergenic 40.0%
CCAAAATGGACCAAAGTTTC+TGG + chr4.1:80029271-80029290 MS.gene50819:CDS 40.0%
CCAAGTTTAAAGGTTCGGAA+AGG + chr4.1:80029792-80029811 MS.gene50819:intron 40.0%
CCTCGAAATTTGGATTCGTA+CGG + chr4.1:80029221-80029240 MS.gene50819:CDS 40.0%
CCTTTCCGAACCTTTAAACT+TGG - chr4.1:80029795-80029814 None:intergenic 40.0%
GAAAAATGCGCTCACAACTT+CGG + chr4.1:80029595-80029614 MS.gene50819:intron 40.0%
GAAGTCAACAAAATCACCGT+TGG - chr4.1:80030237-80030256 None:intergenic 40.0%
GATGGTGTATCAGTTAGTGT+CGG + chr4.1:80029713-80029732 MS.gene50819:intron 40.0%
GGATTGAGTAACACAGAAAC+AGG - chr4.1:80030368-80030387 None:intergenic 40.0%
GTAACACAGAAACAGGGTTA+AGG - chr4.1:80030361-80030380 None:intergenic 40.0%
GTAGTAGTACTAGGGTTTGA+TGG + chr4.1:80029695-80029714 MS.gene50819:intron 40.0%
GTGATATATGCAGAAGCCAA+CGG + chr4.1:80030218-80030237 MS.gene50819:CDS 40.0%
GTTAGATTAATGCCTTGCAC+CGG + chr4.1:80030143-80030162 MS.gene50819:CDS 40.0%
TACAATGGATCCTAATGGCA+TGG - chr4.1:80030276-80030295 None:intergenic 40.0%
TCAACATTCCTCAGATTTCG+CGG + chr4.1:80030493-80030512 MS.gene50819:CDS 40.0%
TCAATCTGAGGAATGTTGAG+TGG - chr4.1:80030425-80030444 None:intergenic 40.0%
TCATCAGACACCAGAAACTT+TGG - chr4.1:80029284-80029303 None:intergenic 40.0%
TCTCCCAACAGGTCAAAAAT+AGG + chr4.1:80030650-80030669 MS.gene50819:CDS 40.0%
TGGGATTTGTGAAGAGAGAA+GGG + chr4.1:80030678-80030697 MS.gene50819:CDS 40.0%
TGTTTCCTTACCATGCCATT+AGG + chr4.1:80030263-80030282 MS.gene50819:CDS 40.0%
TTACTTATGTTACCGGTGCA+AGG - chr4.1:80030158-80030177 None:intergenic 40.0%
TTCATGCACAGTGATTCACA+TGG - chr4.1:80029080-80029099 None:intergenic 40.0%
TTCTTTACTCGGCGTGATAT+CGG + chr4.1:80031206-80031225 MS.gene50819:CDS 40.0%
TTGATGCAGATTGCTTAGGT+AGG - chr4.1:80030121-80030140 None:intergenic 40.0%
TTGATGTCAAGAATGCTCCA+AGG - chr4.1:80030930-80030949 None:intergenic 40.0%
! AACTCTTTTGCTGGATGTGT+TGG + chr4.1:80030311-80030330 MS.gene50819:CDS 40.0%
! ATCATTGTTGCTGTTGATGG+TGG - chr4.1:80028922-80028941 None:intergenic 40.0%
! ATTAACCTTGACTAGTGGAC+TGG + chr4.1:80030951-80030970 MS.gene50819:CDS 40.0%
! CAGGTCAAAAATAGGTGTTG+TGG + chr4.1:80030658-80030677 MS.gene50819:CDS 40.0%
! CCAGAAACTTTGGTCCATTT+TGG - chr4.1:80029274-80029293 None:intergenic 40.0%
! GATGATGGTACTCCAAACAA+TGG - chr4.1:80031417-80031436 None:intergenic 40.0%
! GTTTCTGTGTTACTCAATCC+TGG + chr4.1:80030368-80030387 MS.gene50819:CDS 40.0%
! TTGCAGCTGTTGATGGAATT+GGG + chr4.1:80029338-80029357 MS.gene50819:CDS 40.0%
! TTGGGATCTAACTCTTTTGC+TGG + chr4.1:80030302-80030321 MS.gene50819:CDS 40.0%
!! GAGAGTGTCAACAAAATCCT+TGG - chr4.1:80028853-80028872 None:intergenic 40.0%
!! GATAAGCATTGGTGAAGTTG+AGG + chr4.1:80030808-80030827 MS.gene50819:CDS 40.0%
!! TTAACCTTGACTAGTGGACT+GGG + chr4.1:80030952-80030971 MS.gene50819:CDS 40.0%
!!! GAATTCTTTTGGGGCAACAT+CGG + chr4.1:80031293-80031312 MS.gene50819:CDS 40.0%
!!! GTGATATCGGCTTCCATTTT+CGG + chr4.1:80031219-80031238 MS.gene50819:CDS 40.0%
AAAGACGGTATAGAAGCTGC+CGG - chr4.1:80031468-80031487 None:intergenic 45.0%
AACAAATGTTGCTGCATCCC+AGG + chr4.1:80029051-80029070 MS.gene50819:CDS 45.0%
AATATTGCGGGTGCCCAAAA+TGG + chr4.1:80029257-80029276 MS.gene50819:CDS 45.0%
ACCTACACTCAGACGATGTT+TGG + chr4.1:80029012-80029031 MS.gene50819:CDS 45.0%
AGCTTCCTATCTCTTCCACT+TGG + chr4.1:80028876-80028895 MS.gene50819:CDS 45.0%
CAGGAACACCAAAGAGAAAG+AGG + chr4.1:80030468-80030487 MS.gene50819:CDS 45.0%
CAGTGATTCACATGGATTCC+TGG - chr4.1:80029072-80029091 None:intergenic 45.0%
CCGTACGAATCCAAATTTCG+AGG - chr4.1:80029224-80029243 None:intergenic 45.0%
CCTTAATTGCACTGGAACCA+AGG + chr4.1:80030627-80030646 MS.gene50819:CDS 45.0%
CCTTGGTTCCAGTGCAATTA+AGG - chr4.1:80030630-80030649 None:intergenic 45.0%
CGGCTTCAAATACAGCTTCA+GGG + chr4.1:80031313-80031332 MS.gene50819:CDS 45.0%
CTTCGGTTATGATGCTGTTG+TGG + chr4.1:80029612-80029631 MS.gene50819:intron 45.0%
GACTTACTTGCAGCTGTTGA+TGG + chr4.1:80029331-80029350 MS.gene50819:CDS 45.0%
GAGAGGCTCATTAGAGGTTA+AGG - chr4.1:80030057-80030076 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG + chr4.1:80029383-80029402 MS.gene50819:CDS 45.0%
GAGGATCCAAAATTGACGGA+AGG - chr4.1:80031186-80031205 None:intergenic 45.0%
GATCCAAAATTGACGGAAGG+AGG - chr4.1:80031183-80031202 None:intergenic 45.0%
GCCTTTGATGTTCCAAACGA+CGG - chr4.1:80031513-80031532 None:intergenic 45.0%
GGAACACCAAAGAGAAAGAG+GGG + chr4.1:80030470-80030489 MS.gene50819:CDS 45.0%
GGCAAAAGGAGAAGAAGTTG+CGG - chr4.1:80031124-80031143 None:intergenic 45.0%
GGTGTACCAGAAGATGATGA+TGG - chr4.1:80031432-80031451 None:intergenic 45.0%
GTGGGATTTGTGAAGAGAGA+AGG + chr4.1:80030677-80030696 MS.gene50819:CDS 45.0%
GTTGCCCCAAAAGAATTCGT+AGG - chr4.1:80031291-80031310 None:intergenic 45.0%
TAAGCTGGGTGTTCAATCTG+AGG - chr4.1:80030437-80030456 None:intergenic 45.0%
TATCACGCCGAGTAAAGAAG+AGG - chr4.1:80031205-80031224 None:intergenic 45.0%
TCCAAACATCGTCTGAGTGT+AGG - chr4.1:80029016-80029035 None:intergenic 45.0%
TCGGCTTCAAATACAGCTTC+AGG + chr4.1:80031312-80031331 MS.gene50819:CDS 45.0%
TCGGCTTCTACACCATTGTT+TGG + chr4.1:80031402-80031421 MS.gene50819:CDS 45.0%
TCTGAGGAATGTTGAGTGGT+TGG - chr4.1:80030421-80030440 None:intergenic 45.0%
TGCGAAGTCCTTAATTGCAC+TGG + chr4.1:80030619-80030638 MS.gene50819:CDS 45.0%
TGGATCCTAATGGCATGGTA+AGG - chr4.1:80030271-80030290 None:intergenic 45.0%
TGTCTGTGAGGATCCAAATG+CGG - chr4.1:80031094-80031113 None:intergenic 45.0%
TGTTGACCCCTCTTTCTCTT+TGG - chr4.1:80030479-80030498 None:intergenic 45.0%
TTCAGACGCGACTCAATTAG+AGG + chr4.1:80029364-80029383 MS.gene50819:CDS 45.0%
! ACCGTCGTTTGGAACATCAA+AGG + chr4.1:80031509-80031528 MS.gene50819:CDS 45.0%
! CATCGTCTGAGTGTAGGTTT+TGG - chr4.1:80029010-80029029 None:intergenic 45.0%
! CTTGCAGCTGTTGATGGAAT+TGG + chr4.1:80029337-80029356 MS.gene50819:CDS 45.0%
! GCAGCTTCTATACCGTCTTT+TGG + chr4.1:80031468-80031487 MS.gene50819:CDS 45.0%
! GGAATGTTGAGTGGTTGGTT+TGG - chr4.1:80030416-80030435 None:intergenic 45.0%
! GGAGTACCATCATCATCTTC+TGG + chr4.1:80031423-80031442 MS.gene50819:CDS 45.0%
! TCTTCTGGTACACCGTCTTT+TGG + chr4.1:80031438-80031457 MS.gene50819:CDS 45.0%
! TGCAGCTGTTGATGGAATTG+GGG + chr4.1:80029339-80029358 MS.gene50819:CDS 45.0%
! TGGTTTGGACAACCAAACTG+TGG - chr4.1:80030401-80030420 None:intergenic 45.0%
!! GTGTAGGCTTTGAACTTCCT+TGG + chr4.1:80030910-80030929 MS.gene50819:intron 45.0%
!! TGCTGTTGATGGTGGCTAAA+AGG - chr4.1:80028914-80028933 None:intergenic 45.0%
!! TGTTGATGGTGGCTAAAAGG+CGG - chr4.1:80028911-80028930 None:intergenic 45.0%
!! TTTCGAGGCTGTTGGTTTGT+TGG - chr4.1:80029209-80029228 None:intergenic 45.0%
!!! ACCGTCTTTTGGAGCTCTAA+CGG + chr4.1:80031479-80031498 MS.gene50819:CDS 45.0%
!!! AGTGTAGGTTTTGGACGGTT+TGG - chr4.1:80029001-80029020 None:intergenic 45.0%
!!! GTCTGAGTGTAGGTTTTGGA+CGG - chr4.1:80029006-80029025 None:intergenic 45.0%
AAATAGCGAGCCAAAGGTGG+CGG - chr4.1:80031247-80031266 None:intergenic 50.0%
AATGCGGAGGTGGATGATGA+AGG - chr4.1:80031078-80031097 None:intergenic 50.0%
ACACAACATTGGCAAGCAGG+AGG + chr4.1:80029394-80029413 MS.gene50819:CDS 50.0%
ACCAAATAGCGAGCCAAAGG+TGG - chr4.1:80031250-80031269 None:intergenic 50.0%
ACCACCAAATAGCGAGCCAA+AGG - chr4.1:80031253-80031272 None:intergenic 50.0%
ACCATTGTTTGGATCCCCAG+CGG + chr4.1:80031539-80031558 MS.gene50819:CDS 50.0%
AGGAGATGGCAAGAAAGAGG+AGG - chr4.1:80031145-80031164 None:intergenic 50.0%
CACACACAACATTGGCAAGC+AGG + chr4.1:80029391-80029410 MS.gene50819:CDS 50.0%
CCAAACGGAGTTGAAGATGC+CGG - chr4.1:80031357-80031376 None:intergenic 50.0%
CCAAAGGACGGTACTCCAAA+CGG - chr4.1:80031372-80031391 None:intergenic 50.0%
CCATCTCCTTCCTCTTCTAG+AGG + chr4.1:80031156-80031175 MS.gene50819:CDS 50.0%
CCTGGTATAGCACCACAGTT+TGG + chr4.1:80030386-80030405 MS.gene50819:CDS 50.0%
GATGACGATGCTCCAAAGGA+CGG - chr4.1:80031384-80031403 None:intergenic 50.0%
GCCGTTAGAGCTCCAAAAGA+CGG - chr4.1:80031483-80031502 None:intergenic 50.0%
GGAAGGAGATGGCAAGAAAG+AGG - chr4.1:80031148-80031167 None:intergenic 50.0%
GGCAAGAAAGAGGAGGCAAA+AGG - chr4.1:80031138-80031157 None:intergenic 50.0%
TCATCATCCACCTCCGCATT+TGG + chr4.1:80031078-80031097 MS.gene50819:CDS 50.0%
TTTGACCTGTTGGGAGACCT+TGG - chr4.1:80030647-80030666 None:intergenic 50.0%
! ACCTTTGGCTCGCTATTTGG+TGG + chr4.1:80031249-80031268 MS.gene50819:CDS 50.0%
! ATTTTCGGTTCCGCCACCTT+TGG + chr4.1:80031234-80031253 MS.gene50819:CDS 50.0%
! CCTCTAGAAGAGGAAGGAGA+TGG - chr4.1:80031159-80031178 None:intergenic 50.0%
! GGACCTCCTTCCGTCAATTT+TGG + chr4.1:80031177-80031196 MS.gene50819:CDS 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG - chr4.1:80028884-80028903 None:intergenic 50.0%
!! AAAGGTGGCGGAACCGAAAA+TGG - chr4.1:80031235-80031254 None:intergenic 50.0%
!! CCAAACTGTGGTGCTATACC+AGG - chr4.1:80030389-80030408 None:intergenic 50.0%
!! GTCCTTTGGAGCATCGTCAT+CGG + chr4.1:80031383-80031402 MS.gene50819:CDS 50.0%
!!! GGTTTTGGACGGTTTGGTAG+AGG - chr4.1:80028995-80029014 None:intergenic 50.0%
!!! TACACCGTCTTTTGGAGCAC+CGG + chr4.1:80031446-80031465 MS.gene50819:CDS 50.0%
ACAGCTTCAGGGTCGTCGTT+TGG + chr4.1:80031324-80031343 MS.gene50819:CDS 55.0%
ACGGCTTCTACACCGTCGTT+TGG + chr4.1:80031498-80031517 MS.gene50819:CDS 55.0%
AGCCGATGACGATGCTCCAA+AGG - chr4.1:80031388-80031407 None:intergenic 55.0%
AGGAGAAGAAGTTGCGGCAG+AGG - chr4.1:80031118-80031137 None:intergenic 55.0%
CCGGCATCTTCAACTCCGTT+TGG + chr4.1:80031354-80031373 MS.gene50819:CDS 55.0%
CGGAGGTGGATGATGAAGGA+GGG - chr4.1:80031074-80031093 None:intergenic 55.0%
CTGTGAGGATCCAAATGCGG+AGG - chr4.1:80031091-80031110 None:intergenic 55.0%
GGTGGCTAAAAGGCGGACAA+TGG - chr4.1:80028904-80028923 None:intergenic 55.0%
GTCTCCCAGTCCACTAGTCA+AGG - chr4.1:80030959-80030978 None:intergenic 55.0%
TGAGGATCCAAATGCGGAGG+TGG - chr4.1:80031088-80031107 None:intergenic 55.0%
TGAGGCATGCTTCACCCTTC+AGG + chr4.1:80029668-80029687 MS.gene50819:intron 55.0%
TGGAACCAAGGTCTCCCAAC+AGG + chr4.1:80030639-80030658 MS.gene50819:CDS 55.0%
! GCCACCTTTGGCTCGCTATT+TGG + chr4.1:80031246-80031265 MS.gene50819:CDS 55.0%
! GGAAGGAGGTCCTCTAGAAG+AGG - chr4.1:80031169-80031188 None:intergenic 55.0%
! GGAGGTCCTCTAGAAGAGGA+AGG - chr4.1:80031165-80031184 None:intergenic 55.0%
! GTCGTCGTTTGGAGCATCAC+CGG + chr4.1:80031335-80031354 MS.gene50819:CDS 55.0%
! TTGCGCCGTCGTTTGGAGTT+TGG + chr4.1:80031565-80031584 MS.gene50819:CDS 55.0%
!! CCGTTTGGAGTACCGTCCTT+TGG + chr4.1:80031369-80031388 MS.gene50819:CDS 55.0%
GCCGCTGGGGATCCAAACAA+TGG - chr4.1:80031543-80031562 None:intergenic 60.0%
GCGGAGGTGGATGATGAAGG+AGG - chr4.1:80031075-80031094 None:intergenic 60.0%
GCTGCCGGTGCTCCAAAAGA+CGG - chr4.1:80031453-80031472 None:intergenic 60.0%
TGCGGCAGAGGTTGTCTGTG+AGG - chr4.1:80031106-80031125 None:intergenic 60.0%
! AGGCGGACAATGGTGCCAAG+TGG - chr4.1:80028894-80028913 None:intergenic 60.0%
AACGACGGCGCAAAAGCCGC+TGG - chr4.1:80031558-80031577 None:intergenic 65.0%
ACGACGGCGCAAAAGCCGCT+GGG - chr4.1:80031557-80031576 None:intergenic 65.0%
! GCGGCTTTTGCGCCGTCGTT+TGG + chr4.1:80031558-80031577 MS.gene50819:CDS 65.0%
CGACGGCGCAAAAGCCGCTG+GGG - chr4.1:80031556-80031575 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80028753 80031593 80028753 ID=MS.gene50819
chr4.1 mRNA 80028753 80031593 80028753 ID=MS.gene50819.t1;Parent=MS.gene50819
chr4.1 exon 80028753 80029415 80028753 ID=MS.gene50819.t1.exon1;Parent=MS.gene50819.t1
chr4.1 CDS 80028753 80029415 80028753 ID=cds.MS.gene50819.t1;Parent=MS.gene50819.t1
chr4.1 exon 80030032 80030829 80030032 ID=MS.gene50819.t1.exon2;Parent=MS.gene50819.t1
chr4.1 CDS 80030032 80030829 80030032 ID=cds.MS.gene50819.t1;Parent=MS.gene50819.t1
chr4.1 exon 80030916 80031593 80030916 ID=MS.gene50819.t1.exon3;Parent=MS.gene50819.t1
chr4.1 CDS 80030916 80031593 80030916 ID=cds.MS.gene50819.t1;Parent=MS.gene50819.t1
Gene Sequence

>MS.gene50819

ATGGCTTCTCAACAACAACAACAACAAGAGTCAAAAACCATTCCCCTTAAAATCGTAGTAGACAAACAAAGCAACAAAGTTATTTTTCTGGAAGCAACCAAGGATTTTGTTGACACTCTCTTTAGCTTCCTATCTCTTCCACTTGGCACCATTGTCCGCCTTTTAGCCACCATCAACAGCAACAATGATCAACAAAACAAACCCTCAGAATCATCACTATTCCTTGAAAACATCAAAAACCTCTACCAAACCGTCCAAAACCTACACTCAGACGATGTTTGGAACACAACTTTTGTCAAACAAATGTTGCTGCATCCCAGGAATCCATGTGAATCACTGTGCATGAAGATGTTCTTGAACGTTGATGACACTGAACCATCAAACAAGTTTTTTGTGTGTGATTCTTGTAACAAGTTCACTACTTTTCAAAACCTTCATTGTACTTGTGGAAAACCAACAAACCAACAGCCTCGAAATTTGGATTCGTACGGTCAAGAGAATAGTAATATTGCGGGTGCCCAAAATGGACCAAAGTTTCTGGTGTCTGATGATTTGAAGATTGTTCCATGTTCGTCAGTGACTTACTTGCAGCTGTTGATGGAATTGGGGTATTCAGACGCGACTCAATTAGAGGAAGTCACACACAACATTGGCAAGCAGGAGGTAATTAATTCTGTTGTGTGGTTTTTTGTTTGATATGTATCTATCTCACAAGTTGTATCATTACATTGTTTTTTTTATTACTTGCATAGCAGCTTAGACCAATCTTAATCACGAATCTTCACCTCATGGTTTTAATTTGTGGTTACTTTTATTGAGAACCTTTATTATGGTAAAATTTAGAAAAATGCGCTCACAACTTCGGTTATGATGCTGTTGTGGAAACCTCTAAAATCATATATTTGTGACTGCAATTGAGGCATGCTTCACCCTTCAGGAAATGTAGTAGTACTAGGGTTTGATGGTGTATCAGTTAGTGTCGGGAACCCGAAAATTAAATAGATCTTAAATTTCAAAGTTGCAACACTAGTAGCTTGTACCAAGTTTAAAGGTTCGGAAAGGAAAAATATTAATTAATATTCACAATCTCAGGATTTAATACTTGACAAGAAAAAGAGAATATAATAAATAAAGAGTAGAATAGTGCAGTTAGGGAAGTCAATTAGTTAGATAAGGATATTGATCACTTTATTGAAAAGTTAATGAAGTTAATTTGTGATTAGTTACTTCCTTTTTTTTGTTCGAAAAAGGTATTCATCACTTCCTTTTTGATGTACAGATATTGAAACTACTCAAGTATACCTTAACCTCTAATGAGCCTCTCACAAATACAATTTTGGCAAGCAGCTCCAAAAATAAAGACAGCCTACCTAAGCAATCTGCATCAACAGTTAGATTAATGCCTTGCACCGGTAACATAAGTAAAATCAATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGGTGATATATGCAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTTGTTTCCTTACCATGCCATTAGGATCCATTGTAAAGCTTTTGGGATCTAACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGGACCTTAACCCTGTTTCTGTGTTACTCAATCCTGGTATAGCACCACAGTTTGGTTGTCCAAACCAACCACTCAACATTCCTCAGATTGAACACCCAGCTTATTATTATGGTACAGGAACACCAAAGAGAAAGAGGGGTCAACATTCCTCAGATTTCGCGGATTATAATGGTAATCGTATAATGATGGATTGTAGTGGTAATACTGTGATAGAAGAAAAGATAGAAGGAGGAGTGATTTCTAAGTCACGCGAATCAATTTATTTTGCGAAGTCCTTAATTGCACTGGAACCAAGGTCTCCCAACAGGTCAAAAATAGGTGTTGTGGGATTTGTGAAGAGAGAAGGGTTATATTGTGTAGGAGATGATCTAAAAGCAAAACCACTTTCTGTTCATTCGCATCTTTCATTTTTGAAAGAGTTGAGCCTTCCTCTTGATGATCTTGAAATGAAGCTGATAAGCATTGGTGAAGTTGAGGTAAAGATGCTATTATTTTATCACTAAGTTTATAAAAATTAATTGTGTTATTTTAACCTCTTTGATTTCCTTTACTTGTGGTGTAGGCTTTGAACTTCCTTGGAGCATTCTTGACATCAAAATTAACCTTGACTAGTGGACTGGGAGACCTTTCGAAACTGCCTAAAATCTCAAACCCCTTTTGCAATCGAACATTCAAAACCCTTCCTCAATTTCAAAACCAAAAGGAAAAAACTTCACACCCTCCTTCATCATCCACCTCCGCATTTGGATCCTCACAGACAACCTCTGCCGCAACTTCTTCTCCTTTTGCCTCCTCTTTCTTGCCATCTCCTTCCTCTTCTAGAGGACCTCCTTCCGTCAATTTTGGATCCTCTTCTTTACTCGGCGTGATATCGGCTTCCATTTTCGGTTCCGCCACCTTTGGCTCGCTATTTGGTGGTAGAGCTCCTACTACTCCTACGAATTCTTTTGGGGCAACATCGGCTTCAAATACAGCTTCAGGGTCGTCGTTTGGAGCATCACCGGCATCTTCAACTCCGTTTGGAGTACCGTCCTTTGGAGCATCGTCATCGGCTTCTACACCATTGTTTGGAGTACCATCATCATCTTCTGGTACACCGTCTTTTGGAGCACCGGCAGCTTCTATACCGTCTTTTGGAGCTCTAACGGCTTCTACACCGTCGTTTGGAACATCAAAGGCTTCTACACCATTGTTTGGATCCCCAGCGGCTTTTGCGCCGTCGTTTGGAGTTTGGTTTTGA
Protein sequence

>MS.gene50819.t1

MASQQQQQQESKTIPLKIVVDKQSNKVIFLEATKDFVDTLFSFLSLPLGTIVRLLATINSNNDQQNKPSESSLFLENIKNLYQTVQNLHSDDVWNTTFVKQMLLHPRNPCESLCMKMFLNVDDTEPSNKFFVCDSCNKFTTFQNLHCTCGKPTNQQPRNLDSYGQENSNIAGAQNGPKFLVSDDLKIVPCSSVTYLQLLMELGYSDATQLEEVTHNIGKQEILKLLKYTLTSNEPLTNTILASSSKNKDSLPKQSASTVRLMPCTGNISKINVKVVQSKSQKKVIYAEANGDFVDFIFCFLTMPLGSIVKLLGSNSFAGCVGNLYKSVEDLNPVSVLLNPGIAPQFGCPNQPLNIPQIEHPAYYYGTGTPKRKRGQHSSDFADYNGNRIMMDCSGNTVIEEKIEGGVISKSRESIYFAKSLIALEPRSPNRSKIGVVGFVKREGLYCVGDDLKAKPLSVHSHLSFLKELSLPLDDLEMKLISIGEVEALNFLGAFLTSKLTLTSGLGDLSKLPKISNPFCNRTFKTLPQFQNQKEKTSHPPSSSTSAFGSSQTTSAATSSPFASSFLPSPSSSRGPPSVNFGSSSLLGVISASIFGSATFGSLFGGRAPTTPTNSFGATSASNTASGSSFGASPASSTPFGVPSFGASSSASTPLFGVPSSSSGTPSFGAPAASIPSFGALTASTPSFGTSKASTPLFGSPAAFAPSFGVWF