AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89795

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89795.t1 MTR_4g017470 58.159 478 103 7 1 382 1 477 2.07e-168 480
MS.gene89795.t1 MTR_4g017410 61.613 310 57 4 125 390 208 499 3.35e-110 332
MS.gene89795.t1 MTR_4g017410 79.070 129 19 3 1 126 1 124 5.25e-60 202
MS.gene89795.t1 MTR_4g017830 43.907 517 161 14 1 389 1 516 4.04e-108 328
MS.gene89795.t1 MTR_4g017550 61.512 291 67 4 126 372 181 470 8.46e-107 323
MS.gene89795.t1 MTR_4g017550 66.019 103 28 3 26 126 3 100 3.08e-33 130
MS.gene89795.t1 MTR_4g017440 55.150 301 90 5 125 381 209 508 6.10e-98 301
MS.gene89795.t1 MTR_4g017440 70.992 131 33 2 1 126 1 131 2.75e-49 174
MS.gene89795.t1 MTR_4g019340 52.265 287 69 6 125 365 239 503 6.87e-78 249
MS.gene89795.t1 MTR_4g019340 62.903 124 34 3 10 126 36 154 8.61e-38 142
MS.gene89795.t1 MTR_4g017610 48.201 278 105 5 125 371 206 475 1.30e-66 219
MS.gene89795.t1 MTR_4g017610 71.429 126 34 2 1 125 1 125 4.41e-53 183
MS.gene89795.t1 MTR_4g019240 55.556 162 54 5 1 158 1 148 8.48e-46 155
MS.gene89795.t1 MTR_4g019240 61.290 62 23 1 333 393 112 173 3.46e-14 70.9
MS.gene89795.t1 MTR_2g029400 31.762 403 209 13 13 363 15 403 1.47e-41 151
MS.gene89795.t1 MTR_4g019310 34.737 285 150 8 126 375 207 490 1.26e-31 125
MS.gene89795.t1 MTR_4g019310 48.780 123 58 2 1 123 1 118 2.54e-28 116
MS.gene89795.t1 MTR_7g066580 27.253 455 220 12 18 375 1 441 2.77e-29 118
MS.gene89795.t1 MTR_7g063920 26.998 463 229 12 1 381 1 436 1.88e-28 115
MS.gene89795.t1 MTR_1g041315 38.889 144 79 3 5 148 7 141 1.52e-23 102
MS.gene89795.t1 MTR_7g066260 48.182 110 48 3 13 122 16 116 3.36e-23 101
MS.gene89795.t1 MTR_7g066360 48.214 112 49 3 13 124 15 117 6.34e-23 100
MS.gene89795.t1 MTR_7g012680 47.414 116 50 4 7 122 11 115 6.69e-23 100
MS.gene89795.t1 MTR_7g058710 41.935 124 51 2 1 124 1 103 8.72e-23 100
MS.gene89795.t1 MTR_7g066570 46.400 125 56 4 1 123 1 116 2.16e-22 99.4
MS.gene89795.t1 MTR_7g066570 26.190 294 163 9 132 379 199 484 6.13e-13 70.5
MS.gene89795.t1 MTR_7g066350 45.968 124 56 4 3 126 2 114 3.74e-22 98.2
MS.gene89795.t1 MTR_7g066290 44.262 122 58 4 1 122 5 116 6.49e-22 97.1
MS.gene89795.t1 MTR_7g066340 44.262 122 55 4 2 123 6 114 7.61e-22 97.4
MS.gene89795.t1 MTR_7g066400 25.773 485 226 18 1 391 1 445 4.61e-20 92.0
MS.gene89795.t1 MTR_7g063840 43.182 132 60 4 7 135 5 124 1.92e-19 90.1
MS.gene89795.t1 MTR_7g063840 27.551 294 159 8 128 381 194 473 3.45e-11 65.1
MS.gene89795.t1 MTR_7g066410 43.243 111 54 3 13 123 11 112 2.10e-19 90.1
MS.gene89795.t1 MTR_7g066410 28.125 320 160 9 123 376 198 513 8.87e-16 79.3
MS.gene89795.t1 MTR_1g014060 45.098 102 47 3 21 122 9 101 5.56e-19 88.2
MS.gene89795.t1 MTR_7g063880 43.651 126 59 4 1 125 1 115 9.46e-19 88.2
MS.gene89795.t1 MTR_7g063880 27.899 276 164 8 128 376 196 463 3.06e-14 74.3
MS.gene89795.t1 MTR_7g063660 41.176 119 59 3 8 125 5 113 8.59e-18 85.1
MS.gene89795.t1 MTR_7g063660 25.503 298 183 5 119 381 184 477 3.65e-12 68.2
MS.gene89795.t1 MTR_7g063900 41.525 118 56 3 10 125 8 114 8.85e-18 85.1
MS.gene89795.t1 MTR_7g066310 44.068 118 55 4 7 124 4 110 1.09e-17 84.3
MS.gene89795.t1 MTR_7g063830 40.000 115 56 3 13 125 11 114 1.39e-17 84.3
MS.gene89795.t1 MTR_7g066490 40.909 132 62 3 13 144 17 132 2.57e-17 83.6
MS.gene89795.t1 MTR_7g066480 45.946 111 51 2 13 123 17 118 5.16e-17 82.8
MS.gene89795.t1 MTR_7g066480 28.090 267 134 7 126 376 198 422 5.64e-11 64.3
MS.gene89795.t1 MTR_7g062300 36.957 138 76 3 1 138 1 127 1.29e-16 81.6
MS.gene89795.t1 MTR_7g062300 29.091 330 168 15 89 374 165 472 1.88e-13 72.0
MS.gene89795.t1 MTR_7g063820 43.651 126 57 5 1 125 1 113 1.45e-16 81.6
MS.gene89795.t1 MTR_7g063890 40.351 114 57 3 13 125 28 131 5.03e-16 80.1
MS.gene89795.t1 MTR_7g062280 30.169 295 156 8 126 381 208 491 7.26e-16 79.3
MS.gene89795.t1 MTR_7g062280 39.823 113 59 2 13 125 11 114 1.44e-14 75.5
MS.gene89795.t1 MTR_7g066430 43.299 97 46 3 13 109 5 92 1.21e-15 79.0
MS.gene89795.t1 MTR_7g066430 26.623 308 169 8 123 377 184 487 6.18e-15 76.6
MS.gene89795.t1 MTR_7g062270 37.500 128 67 4 1 127 1 116 2.22e-15 77.4
MS.gene89795.t1 MTR_7g063690 32.240 183 87 4 1 160 1 169 2.95e-15 75.1
MS.gene89795.t1 MTR_7g058700 39.837 123 43 4 10 124 509 608 1.43e-13 72.8
MS.gene89795.t1 MTR_6g006800 72.340 47 12 1 1 47 1 46 1.76e-13 65.5
MS.gene89795.t1 MTR_4g019350 45.946 74 38 1 223 294 10 83 9.35e-13 64.3
MS.gene89795.t1 MTR_1g115440 35.246 122 64 4 13 134 12 118 1.89e-12 67.0
MS.gene89795.t1 MTR_4g019330 32.370 173 64 5 211 381 67 188 4.67e-12 64.7
MS.gene89795.t1 MTR_1g115450 34.211 114 61 2 13 126 8 107 7.51e-12 63.2
MS.gene89795.t1 MTR_7g063670 37.795 127 54 3 1 125 4 107 1.66e-11 65.9
MS.gene89795.t1 MTR_0150s0010 35.156 128 67 4 1 128 1 112 1.79e-11 63.9
MS.gene89795.t1 MTR_7g100420 67.442 43 14 0 12 54 39 81 2.00e-11 65.5
MS.gene89795.t1 MTR_1g115455 37.634 93 44 2 13 105 10 88 3.63e-11 63.2
MS.gene89795.t1 MTR_3g065200 37.600 125 53 5 13 131 4 109 8.58e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89795.t1 AT5G01140 24.736 473 222 16 15 381 12 456 6.16e-21 94.7
MS.gene89795.t1 AT5G01150 44.037 109 52 3 15 123 12 111 2.75e-18 87.0
MS.gene89795.t1 AT5G01150 24.739 287 179 6 125 376 196 480 1.58e-11 66.2
MS.gene89795.t1 AT5G01130 41.071 112 55 3 13 123 10 111 6.01e-18 85.5
MS.gene89795.t1 AT5G01130 27.178 287 141 11 126 375 189 444 3.27e-14 74.3
MS.gene89795.t1 AT3G09110 39.496 119 63 3 9 127 6 115 1.68e-17 83.6
MS.gene89795.t1 AT5G43240 44.248 113 54 3 13 125 10 113 4.56e-17 83.2
MS.gene89795.t1 AT5G43240 26.452 310 167 9 113 371 190 489 1.68e-11 66.2
MS.gene89795.t1 AT5G43240 44.248 113 54 3 13 125 10 113 4.56e-17 83.2
MS.gene89795.t1 AT5G43240 26.452 310 167 9 113 371 190 489 1.68e-11 66.2
MS.gene89795.t1 AT5G43240 44.248 113 54 3 13 125 10 113 4.56e-17 83.2
MS.gene89795.t1 AT5G43240 26.452 310 167 9 113 371 190 489 1.68e-11 66.2
MS.gene89795.t1 AT3G09120 40.351 114 56 3 13 125 7 109 4.85e-17 81.6
MS.gene89795.t1 AT3G09140 40.179 112 58 3 11 122 8 110 1.02e-15 79.0
MS.gene89795.t1 AT3G09140 40.179 112 58 3 11 122 8 110 1.12e-15 79.0
MS.gene89795.t1 AT5G01120 40.708 113 58 3 13 125 10 113 4.67e-15 77.0
MS.gene89795.t1 AT5G01120 25.763 295 179 8 117 371 191 485 3.16e-14 74.7
MS.gene89795.t1 AT5G37320 27.972 286 151 10 129 376 212 480 6.75e-14 73.6
MS.gene89795.t1 AT5G37320 38.318 107 56 4 19 125 18 114 5.16e-12 67.8
MS.gene89795.t1 AT5G43240 26.433 314 170 9 109 371 42 345 1.14e-11 66.2

Find 77 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGCTTCACATGGATTTC+TGG 0.117643 4.4:+83382851 None:intergenic
CATCATTTGAATTGAGATTT+TGG 0.129092 4.4:+83382913 None:intergenic
AGGCTCCATTGTAAAGCTTT+TGG 0.220130 4.4:-83381377 MS.gene89795:CDS
GTCATTGTTGTTGTTATTCC+TGG 0.237924 4.4:+83382992 None:intergenic
AACAGAACAGAAGGTGTTAT+TGG 0.249994 4.4:-83381042 MS.gene89795:CDS
AGGTCGTTTGTCTTTGTTCT+TGG 0.253669 4.4:+83381558 None:intergenic
CACCAGTGACAACAGGAAAT+TGG 0.303337 4.4:-83381500 MS.gene89795:CDS
TCTGTTCTGTTGAGGGATCT+AGG 0.304846 4.4:+83381054 None:intergenic
TATTCCTGGTGGTGGCTAAA+AGG 0.313041 4.4:+83383006 None:intergenic
ATCTCAATTCAAATGATGTT+TGG 0.325115 4.4:-83382908 MS.gene89795:CDS
GTGGGTTGTGGTTGAACTTC+AGG 0.343664 4.4:+83381226 None:intergenic
AGCGAAACCTTTGATTGAAC+TGG 0.350563 4.4:-83381079 MS.gene89795:CDS
AATGCTTCACATGGATTTCT+GGG 0.382524 4.4:+83382852 None:intergenic
GTGAAGAGAGTATCATTATA+TGG 0.388943 4.4:-83381015 MS.gene89795:CDS
CAACCCACTACATATTACTA+TGG 0.391195 4.4:-83381211 MS.gene89795:intron
GTGTAGGCTTTGAACTTCCT+TGG 0.395429 4.4:-83380801 MS.gene89795:intron
AACAGTAGAAGTAAAGATAG+AGG 0.408680 4.4:-83381139 MS.gene89795:CDS
CAAAGGCTCATGAGAGGTTA+AGG 0.411865 4.4:+83381606 None:intergenic
ACTGCTGATGCAAGTTGGTT+AGG 0.427676 4.4:+83381538 None:intergenic
TTCAGATTTGGCTCAAATAG+AGG 0.433676 4.4:-83382547 MS.gene89795:intron
AATTCCCCTCAAAATGTTAG+TGG 0.442870 4.4:-83383126 MS.gene89795:CDS
AGAGTATCATTATATGGTAT+AGG 0.444006 4.4:-83381009 MS.gene89795:CDS
CTGTTCTGTTGAGGGATCTA+GGG 0.449379 4.4:+83381055 None:intergenic
AACACCTTCTGTTCTGTTGA+GGG 0.450662 4.4:+83381047 None:intergenic
TGCCTTGCACCAGTGACAAC+AGG 0.457008 4.4:-83381507 MS.gene89795:CDS
ATGGTATTGATTCTGATTGT+TGG 0.459184 4.4:+83382970 None:intergenic
CTGGTTTAGGCACACTCAAA+AGG 0.467866 4.4:+83380733 None:intergenic
GCTGGTTAAGACAACTAAAC+AGG 0.478360 4.4:+83381260 None:intergenic
ATCCAATTTCCTGTTGTCAC+TGG 0.480035 4.4:+83381498 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4.4:+83383036 None:intergenic
AGCTTCCTATCTCTTCCACT+TGG 0.491188 4.4:-83383041 MS.gene89795:CDS
AGGAGTGATTTCTAAGTCAC+AGG 0.492064 4.4:-83381115 MS.gene89795:CDS
AAGTCTGTGTTACTCGATCC+TGG 0.493339 4.4:-83381292 MS.gene89795:CDS
GTTGTTGTTATTCCTGGTGG+TGG 0.496331 4.4:+83382998 None:intergenic
GTACAATAGTGTGAAGAAGT+TGG 0.515165 4.4:-83381320 MS.gene89795:CDS
GGATCGAGTAACACAGACTT+GGG 0.525345 4.4:+83381295 None:intergenic
ACAGTAGAAGTAAAGATAGA+GGG 0.526753 4.4:-83381138 MS.gene89795:CDS
AGGAAACAATTAGCAGAAAG+TGG 0.527592 4.4:+83380967 None:intergenic
CTTCAGGAATGTTGACGGGC+TGG 0.530155 4.4:+83381242 None:intergenic
GAGAGTGTCAACAAAGTCCT+TGG 0.531473 4.4:+83383067 None:intergenic
CTCTAACTGCTGATGCAAGT+TGG 0.535856 4.4:+83381533 None:intergenic
CTTGAAGATCATCAAGAGGA+TGG 0.544525 4.4:+83380923 None:intergenic
TAACACCTTCTGTTCTGTTG+AGG 0.544915 4.4:+83381046 None:intergenic
CTAAACAGGTGTGCTATACC+AGG 0.546199 4.4:+83381274 None:intergenic
TAGTAATATGTAGTGGGTTG+TGG 0.549518 4.4:+83381214 None:intergenic
TTGAACTTCAGGAATGTTGA+CGG 0.551725 4.4:+83381237 None:intergenic
AGTTCCCAAAAGCTTTACAA+TGG 0.556042 4.4:+83381372 None:intergenic
TTTCTGATGTTGTCAAGGAA+TGG 0.556391 4.4:+83382951 None:intergenic
TTGTAGCTGTTCAGAAGCCA+TGG 0.558598 4.4:+83383163 None:intergenic
CAAGTGAAGGTGATAAGCAT+TGG 0.564667 4.4:-83380904 MS.gene89795:CDS
TTCCTGTTGTCACTGGTGCA+AGG 0.566131 4.4:+83381505 None:intergenic
CTAGGGTCCAGTTCAATCAA+AGG 0.572776 4.4:+83381072 None:intergenic
TCTTGATGATCTTCAAGTGA+AGG 0.577520 4.4:-83380917 MS.gene89795:CDS
TGAACTTCAGGAATGTTGAC+GGG 0.580572 4.4:+83381238 None:intergenic
AGGATCGAGTAACACAGACT+TGG 0.584803 4.4:+83381294 None:intergenic
GTCTTTAAGTCCACTCGTCA+AGG 0.585990 4.4:+83380755 None:intergenic
AGAGGTTTCTGATGTTGTCA+AGG 0.592804 4.4:+83382946 None:intergenic
GTAGAAGTAAAGATAGAGGG+AGG 0.594513 4.4:-83381135 MS.gene89795:CDS
AGATCCCTCAACAGAACAGA+AGG 0.594691 4.4:-83381051 MS.gene89795:CDS
TCAAGATTTACCTTGACGAG+TGG 0.605006 4.4:-83380765 MS.gene89795:CDS
TTCATGAACAATGCTTCACA+TGG 0.606502 4.4:+83382843 None:intergenic
ATTGTTGTTGTTATTCCTGG+TGG 0.607529 4.4:+83382995 None:intergenic
TTCACTTGAAGATCATCAAG+AGG 0.608876 4.4:+83380919 None:intergenic
AAATGTTAGTGGACAAACAA+AGG 0.609948 4.4:-83383115 MS.gene89795:CDS
GTACCATAGTAATATGTAGT+GGG 0.610428 4.4:+83381208 None:intergenic
GAGGAAGTCACACACAACAT+TGG 0.621785 4.4:-83382528 MS.gene89795:CDS
TGTACCATAGTAATATGTAG+TGG 0.628329 4.4:+83381207 None:intergenic
ACACAACATTGGCAAACAAG+AGG 0.630703 4.4:-83382517 MS.gene89795:intron
AGGTTAAGGTATGCTTGAGG+AGG 0.633116 4.4:+83381620 None:intergenic
GATAAGCATTGGTGAAGCTG+AGG 0.638424 4.4:-83380893 MS.gene89795:intron
ATTTGTCAAAGGCTCATGAG+AGG 0.656727 4.4:+83381600 None:intergenic
GGAGTGATTTCTAAGTCACA+GGG 0.668864 4.4:-83381114 MS.gene89795:CDS
TTGATGTCAAGAATGCTCCA+AGG 0.669048 4.4:+83380784 None:intergenic
GAGAGGTTAAGGTATGCTTG+AGG 0.679201 4.4:+83381617 None:intergenic
AGGCGAACGATGGTGCCAAG+TGG 0.699497 4.4:+83383026 None:intergenic
GGTGGCTAAAAGGCGAACGA+TGG 0.723514 4.4:+83383016 None:intergenic
GATCGAGTAACACAGACTTG+GGG 0.769489 4.4:+83381296 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATAACAAAAATATATTTAG+TGG + chr4.4:83383004-83383023 None:intergenic 10.0%
!!! TTAATTTTTTCAAATATGAA+AGG + chr4.4:83382909-83382928 None:intergenic 10.0%
!! AATTAACAAATTCAATGTAA+TGG + chr4.4:83381409-83381428 None:intergenic 15.0%
!! AATTAATATTCACAATCATT+TGG - chr4.4:83381425-83381444 MS.gene89795:intron 15.0%
!! ACAAAAATAAAGAAATACTA+TGG - chr4.4:83381600-83381619 MS.gene89795:CDS 15.0%
!! ATTAACAAATTCAATGTAAT+GGG + chr4.4:83381408-83381427 None:intergenic 15.0%
!! CAAAAATAAAGAAATACTAT+GGG - chr4.4:83381601-83381620 MS.gene89795:CDS 15.0%
!! AATATCAAATAATAGTCGTA+TGG + chr4.4:83381783-83381802 None:intergenic 20.0%
!! ACAATTAAAGTGAATCAAAA+AGG + chr4.4:83383031-83383050 None:intergenic 20.0%
!!! AACTTTGAAAAAATTGATTC+TGG + chr4.4:83383142-83383161 None:intergenic 20.0%
!!! TTTTTGATTCACTTTAATTG+TGG - chr4.4:83383030-83383049 MS.gene89795:CDS 20.0%
!!! TTTTTTCAAAGTTCCTAAAA+AGG - chr4.4:83383149-83383168 MS.gene89795:CDS 20.0%
! ACAAGAAAAAGCAATACTAT+GGG - chr4.4:83381652-83381671 MS.gene89795:intron 25.0%
! ATCTCAATTCAAATGATGTT+TGG - chr4.4:83380945-83380964 MS.gene89795:CDS 25.0%
! CATTTACCAAACATTCTTAT+CGG + chr4.4:83381721-83381740 None:intergenic 25.0%
! GCAAAAAATGTATTTGTCAA+AGG + chr4.4:83382267-83382286 None:intergenic 25.0%
! TACAAGAAAAAGCAATACTA+TGG - chr4.4:83381651-83381670 MS.gene89795:intron 25.0%
!! AGAGTATCATTATATGGTAT+AGG - chr4.4:83382844-83382863 MS.gene89795:CDS 25.0%
!! ATTTGAATTGAGATTTTGGA+CGG + chr4.4:83380939-83380958 None:intergenic 25.0%
!! CATCATTTGAATTGAGATTT+TGG + chr4.4:83380943-83380962 None:intergenic 25.0%
!! TCTTATAAAGACCTCATTTT+GGG + chr4.4:83381194-83381213 None:intergenic 25.0%
!! TTTTTCTGTTAGAAAACATG+TGG - chr4.4:83382044-83382063 MS.gene89795:intron 25.0%
!!! AGTATTGCTTTTTCTTGTAT+TGG + chr4.4:83381650-83381669 None:intergenic 25.0%
!!! GAAAAAATTGATTCTGGTTT+AGG + chr4.4:83383136-83383155 None:intergenic 25.0%
!!! GTATTGCTTTTTCTTGTATT+GGG + chr4.4:83381649-83381668 None:intergenic 25.0%
AACAGTAGAAGTAAAGATAG+AGG - chr4.4:83382714-83382733 MS.gene89795:intron 30.0%
ACAGTAGAAGTAAAGATAGA+GGG - chr4.4:83382715-83382734 MS.gene89795:intron 30.0%
ACTACATATTACTATGGTAC+AGG - chr4.4:83382648-83382667 MS.gene89795:intron 30.0%
AGAAAATGGATCAATGTTTC+TGG - chr4.4:83381213-83381232 MS.gene89795:CDS 30.0%
ATAATCTTTGCAGAAACCAA+TGG - chr4.4:83382411-83382430 MS.gene89795:intron 30.0%
ATTCACTTTAATTGTGGTGT+AGG - chr4.4:83383036-83383055 MS.gene89795:CDS 30.0%
CACACAAAAAACTTACTTGA+TGG + chr4.4:83381064-83381083 None:intergenic 30.0%
CTACATATTACTATGGTACA+GGG - chr4.4:83382649-83382668 MS.gene89795:intron 30.0%
GAGGTCTTTATAAGAGAAAA+TGG - chr4.4:83381199-83381218 MS.gene89795:intron 30.0%
GTACCATAGTAATATGTAGT+GGG + chr4.4:83382648-83382667 None:intergenic 30.0%
GTGAATAAGCAGTAAATTCA+AGG + chr4.4:83381545-83381564 None:intergenic 30.0%
TAATAGATAGCCTAAGAACT+TGG - chr4.4:83381144-83381163 MS.gene89795:CDS 30.0%
TACTGCTTATTCACCTTAAA+GGG - chr4.4:83381551-83381570 MS.gene89795:CDS 30.0%
TGTACCATAGTAATATGTAG+TGG + chr4.4:83382649-83382668 None:intergenic 30.0%
TTACTGCTTATTCACCTTAA+AGG - chr4.4:83381550-83381569 MS.gene89795:CDS 30.0%
TTCTTAGGCTATCTATTAGT+GGG + chr4.4:83381142-83381161 None:intergenic 30.0%
! AAATGTTAGTGGACAAACAA+AGG - chr4.4:83380738-83380757 MS.gene89795:CDS 30.0%
! ATTTTTGTCAATTAGCCAAG+TGG + chr4.4:83381588-83381607 None:intergenic 30.0%
! CTCTTATAAAGACCTCATTT+TGG + chr4.4:83381195-83381214 None:intergenic 30.0%
! GTTTTCTAACAGAAAAATGC+AGG + chr4.4:83382041-83382060 None:intergenic 30.0%
! TTTGTCCACTAACATTTTGA+GGG + chr4.4:83380735-83380754 None:intergenic 30.0%
!! ATGGTATTGATTCTGATTGT+TGG + chr4.4:83380886-83380905 None:intergenic 30.0%
!! GTGAAGAGAGTATCATTATA+TGG - chr4.4:83382838-83382857 MS.gene89795:CDS 30.0%
!!! AAGGAACAAAGTTGTTTTTG+TGG - chr4.4:83380757-83380776 MS.gene89795:CDS 30.0%
AAAAATCGCATCTAAGCACA+AGG - chr4.4:83381868-83381887 MS.gene89795:intron 35.0%
AATGCTTCACATGGATTTCT+GGG + chr4.4:83381004-83381023 None:intergenic 35.0%
AATTCCCCTCAAAATGTTAG+TGG - chr4.4:83380727-83380746 MS.gene89795:CDS 35.0%
ACTGAAACATCTCTTCTTAG+AGG - chr4.4:83382078-83382097 MS.gene89795:intron 35.0%
AGGAAACAATTAGCAGAAAG+TGG + chr4.4:83382889-83382908 None:intergenic 35.0%
AGTTCCCAAAAGCTTTACAA+TGG + chr4.4:83382484-83382503 None:intergenic 35.0%
ATCTGTGATCACAACTTAGT+TGG - chr4.4:83381485-83381504 MS.gene89795:CDS 35.0%
CAACCCACTACATATTACTA+TGG - chr4.4:83382642-83382661 MS.gene89795:intron 35.0%
CGATCTTATCTTCTCATCTT+AGG + chr4.4:83382017-83382036 None:intergenic 35.0%
GAAGTCAACAAAATCTCCAT+TGG + chr4.4:83382430-83382449 None:intergenic 35.0%
GTACAATAGTGTGAAGAAGT+TGG - chr4.4:83382533-83382552 MS.gene89795:CDS 35.0%
GTTCTTAGGCTATCTATTAG+TGG + chr4.4:83381143-83381162 None:intergenic 35.0%
TTCACTTGAAGATCATCAAG+AGG + chr4.4:83382937-83382956 None:intergenic 35.0%
TTCATGAACAATGCTTCACA+TGG + chr4.4:83381013-83381032 None:intergenic 35.0%
TTGAACTTCAGGAATGTTGA+CGG + chr4.4:83382619-83382638 None:intergenic 35.0%
TTTCTGATGTTGTCAAGGAA+TGG + chr4.4:83380905-83380924 None:intergenic 35.0%
! AATTGAGATTTTGGACGGTA+CGG + chr4.4:83380934-83380953 None:intergenic 35.0%
! ATTGTTGTTGTTATTCCTGG+TGG + chr4.4:83380861-83380880 None:intergenic 35.0%
! GTCATTGTTGTTGTTATTCC+TGG + chr4.4:83380864-83380883 None:intergenic 35.0%
! GTTTGTCCACTAACATTTTG+AGG + chr4.4:83380736-83380755 None:intergenic 35.0%
! TCTTGATGATCTTCAAGTGA+AGG - chr4.4:83382936-83382955 MS.gene89795:CDS 35.0%
! TGATCACAACTTAGTTGGTA+GGG - chr4.4:83381490-83381509 MS.gene89795:CDS 35.0%
! TGGGAACTAACTCTTTTGAT+GGG - chr4.4:83382496-83382515 MS.gene89795:intron 35.0%
! TTCAGATTTGGCTCAAATAG+AGG - chr4.4:83381306-83381325 MS.gene89795:CDS 35.0%
! TTGAAGCTGTTGATGGAATT+GGG - chr4.4:83381280-83381299 MS.gene89795:CDS 35.0%
! TTGGGAACTAACTCTTTTGA+TGG - chr4.4:83382495-83382514 MS.gene89795:intron 35.0%
! TTGTCCACTAACATTTTGAG+GGG + chr4.4:83380734-83380753 None:intergenic 35.0%
!! AACAGAACAGAAGGTGTTAT+TGG - chr4.4:83382811-83382830 MS.gene89795:CDS 35.0%
!! TAAGCACCGATAAGAATGTT+TGG - chr4.4:83381712-83381731 MS.gene89795:intron 35.0%
!! TAGTAATATGTAGTGGGTTG+TGG + chr4.4:83382642-83382661 None:intergenic 35.0%
AACACCTTCTGTTCTGTTGA+GGG + chr4.4:83382809-83382828 None:intergenic 40.0%
AAGCAAATCCATCGTAATCC+TGG + chr4.4:83382114-83382133 None:intergenic 40.0%
AAGTAAGTCACAGAGGAACT+TGG + chr4.4:83381262-83381281 None:intergenic 40.0%
ACACAACATTGGCAAACAAG+AGG - chr4.4:83381336-83381355 MS.gene89795:CDS 40.0%
AGAGGTTTCTGATGTTGTCA+AGG + chr4.4:83380910-83380929 None:intergenic 40.0%
AGAGTAAGGTCACTAGGTTT+GGG - chr4.4:83381991-83382010 MS.gene89795:intron 40.0%
AGCGAAACCTTTGATTGAAC+TGG - chr4.4:83382774-83382793 MS.gene89795:intron 40.0%
AGCTTTACAATGGAGCCTAA+TGG + chr4.4:83382474-83382493 None:intergenic 40.0%
AGGAGTGATTTCTAAGTCAC+AGG - chr4.4:83382738-83382757 MS.gene89795:intron 40.0%
AGGTCGTTTGTCTTTGTTCT+TGG + chr4.4:83382298-83382317 None:intergenic 40.0%
ATCCAATTTCCTGTTGTCAC+TGG + chr4.4:83382358-83382377 None:intergenic 40.0%
ATTTGTCAAAGGCTCATGAG+AGG + chr4.4:83382256-83382275 None:intergenic 40.0%
CAAGTGAAGGTGATAAGCAT+TGG - chr4.4:83382949-83382968 MS.gene89795:CDS 40.0%
CAATGCTTCACATGGATTTC+TGG + chr4.4:83381005-83381024 None:intergenic 40.0%
CCTAAGAACTTGGATTCTGA+GGG - chr4.4:83381154-83381173 MS.gene89795:intron 40.0%
CTTGAAGATCATCAAGAGGA+TGG + chr4.4:83382933-83382952 None:intergenic 40.0%
GACTTACTTGAAGCTGTTGA+TGG - chr4.4:83381273-83381292 MS.gene89795:CDS 40.0%
GCTGGTTAAGACAACTAAAC+AGG + chr4.4:83382596-83382615 None:intergenic 40.0%
GGAGTGATTTCTAAGTCACA+GGG - chr4.4:83382739-83382758 MS.gene89795:intron 40.0%
GTAGAAGTAAAGATAGAGGG+AGG - chr4.4:83382718-83382737 MS.gene89795:intron 40.0%
GTGATCACAACTTAGTTGGT+AGG - chr4.4:83381489-83381508 MS.gene89795:CDS 40.0%
TAACACCTTCTGTTCTGTTG+AGG + chr4.4:83382810-83382829 None:intergenic 40.0%
TCAAGATTTACCTTGACGAG+TGG - chr4.4:83383088-83383107 MS.gene89795:CDS 40.0%
TGAACTTCAGGAATGTTGAC+GGG + chr4.4:83382618-83382637 None:intergenic 40.0%
TGAGAGTAATGCCCAAAATG+AGG - chr4.4:83381180-83381199 MS.gene89795:intron 40.0%
TTGATGTCAAGAATGCTCCA+AGG + chr4.4:83383072-83383091 None:intergenic 40.0%
! AGGCTCCATTGTAAAGCTTT+TGG - chr4.4:83382476-83382495 MS.gene89795:intron 40.0%
! CTTGAAGCTGTTGATGGAAT+TGG - chr4.4:83381279-83381298 MS.gene89795:CDS 40.0%
! GGAATTGGGGTATTCAGATT+TGG - chr4.4:83381294-83381313 MS.gene89795:CDS 40.0%
! GGCTCCATTGTAAAGCTTTT+GGG - chr4.4:83382477-83382496 MS.gene89795:intron 40.0%
! TGAAGCTGTTGATGGAATTG+GGG - chr4.4:83381281-83381300 MS.gene89795:CDS 40.0%
!! AACTCTTTTGATGGGTGTGT+TGG - chr4.4:83382504-83382523 MS.gene89795:intron 40.0%
!! TGTTTTTGTGGAAGCAACCA+AGG - chr4.4:83380769-83380788 MS.gene89795:CDS 40.0%
!!! TCTATTAGTGGGTTTTCCAC+AGG + chr4.4:83381131-83381150 None:intergenic 40.0%
AATCCATCGTAATCCTGGAG+TGG + chr4.4:83382109-83382128 None:intergenic 45.0%
AGATCCCTCAACAGAACAGA+AGG - chr4.4:83382802-83382821 MS.gene89795:intron 45.0%
AGCTTCCTATCTCTTCCACT+TGG - chr4.4:83380812-83380831 MS.gene89795:intron 45.0%
AGCTTCCTTACCATGCCATT+AGG - chr4.4:83382456-83382475 MS.gene89795:intron 45.0%
AGGATCGAGTAACACAGACT+TGG + chr4.4:83382562-83382581 None:intergenic 45.0%
AGGTTAAGGTATGCTTGAGG+AGG + chr4.4:83382236-83382255 None:intergenic 45.0%
CAAAGGCTCATGAGAGGTTA+AGG + chr4.4:83382250-83382269 None:intergenic 45.0%
CAAGGTTCAGACACCAACTA+GGG + chr4.4:83381527-83381546 None:intergenic 45.0%
CACCAGTGACAACAGGAAAT+TGG - chr4.4:83382353-83382372 MS.gene89795:intron 45.0%
CAGAATCTTGATTGCACCTG+TGG - chr4.4:83381112-83381131 MS.gene89795:CDS 45.0%
CAGAGTAAGGTCACTAGGTT+TGG - chr4.4:83381990-83382009 MS.gene89795:intron 45.0%
CAGCTTCAAGTAAGTCACAG+AGG + chr4.4:83381269-83381288 None:intergenic 45.0%
CATGTCAGAGTAAGGTCACT+AGG - chr4.4:83381985-83382004 MS.gene89795:intron 45.0%
CCCTCAGAATCCAAGTTCTT+AGG + chr4.4:83381157-83381176 None:intergenic 45.0%
CTAAACAGGTGTGCTATACC+AGG + chr4.4:83382582-83382601 None:intergenic 45.0%
CTAGGGTCCAGTTCAATCAA+AGG + chr4.4:83382784-83382803 None:intergenic 45.0%
CTAGTGACCTTACTCTGACA+TGG + chr4.4:83381987-83382006 None:intergenic 45.0%
CTCTAACTGCTGATGCAAGT+TGG + chr4.4:83382323-83382342 None:intergenic 45.0%
CTGGTTTAGGCACACTCAAA+AGG + chr4.4:83383123-83383142 None:intergenic 45.0%
CTGTTCTGTTGAGGGATCTA+GGG + chr4.4:83382801-83382820 None:intergenic 45.0%
GAGAGGTTAAGGTATGCTTG+AGG + chr4.4:83382239-83382258 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG - chr4.4:83381325-83381344 MS.gene89795:CDS 45.0%
GATCGAGTAACACAGACTTG+GGG + chr4.4:83382560-83382579 None:intergenic 45.0%
GCCTAAGAACTTGGATTCTG+AGG - chr4.4:83381153-83381172 MS.gene89795:intron 45.0%
GGATCGAGTAACACAGACTT+GGG + chr4.4:83382561-83382580 None:intergenic 45.0%
GTCTTTAAGTCCACTCGTCA+AGG + chr4.4:83383101-83383120 None:intergenic 45.0%
TACAATGGAGCCTAATGGCA+TGG + chr4.4:83382469-83382488 None:intergenic 45.0%
TCAAGGTTCAGACACCAACT+AGG + chr4.4:83381528-83381547 None:intergenic 45.0%
TCTGTTCTGTTGAGGGATCT+AGG + chr4.4:83382802-83382821 None:intergenic 45.0%
TTGGTGAAGCTGAGGTAATG+TGG - chr4.4:83382968-83382987 MS.gene89795:CDS 45.0%
! AAGTCTGTGTTACTCGATCC+TGG - chr4.4:83382561-83382580 MS.gene89795:intron 45.0%
! GTTGTTGTTATTCCTGGTGG+TGG + chr4.4:83380858-83380877 None:intergenic 45.0%
!! ACTGCTGATGCAAGTTGGTT+AGG + chr4.4:83382318-83382337 None:intergenic 45.0%
!! GAGAGTGTCAACAAAGTCCT+TGG + chr4.4:83380789-83380808 None:intergenic 45.0%
!! GATAAGCATTGGTGAAGCTG+AGG - chr4.4:83382960-83382979 MS.gene89795:CDS 45.0%
!! GTGTAGGCTTTGAACTTCCT+TGG - chr4.4:83383052-83383071 MS.gene89795:CDS 45.0%
!! TATTCCTGGTGGTGGCTAAA+AGG + chr4.4:83380850-83380869 None:intergenic 45.0%
!!! TATTATTTGATATTTTATAA+TGG - chr4.4:83381789-83381808 MS.gene89795:intron 5.0%
ACCTTACTCTGACATGGTGG+TGG + chr4.4:83381981-83382000 None:intergenic 50.0%
AGGTGTGCATTGTGCAAGGA+TGG - chr4.4:83381888-83381907 MS.gene89795:intron 50.0%
CACAAGGTGTGCATTGTGCA+AGG - chr4.4:83381884-83381903 MS.gene89795:intron 50.0%
CCTTACTCTGACATGGTGGT+GGG + chr4.4:83381980-83381999 None:intergenic 50.0%
CTTAGAGGAACTTCCACTCC+AGG - chr4.4:83382093-83382112 MS.gene89795:intron 50.0%
CTTCCACTCCAGGATTACGA+TGG - chr4.4:83382103-83382122 MS.gene89795:intron 50.0%
GGCTAGAGTCTCACCCTTTA+AGG + chr4.4:83381567-83381586 None:intergenic 50.0%
GTGACCTTACTCTGACATGG+TGG + chr4.4:83381984-83382003 None:intergenic 50.0%
TGGAGCCTAATGGCATGGTA+AGG + chr4.4:83382464-83382483 None:intergenic 50.0%
TTCCTGTTGTCACTGGTGCA+AGG + chr4.4:83382351-83382370 None:intergenic 50.0%
! GATTTTGGACGGTACGGTAG+AGG + chr4.4:83380928-83380947 None:intergenic 50.0%
! GTGGGTTGTGGTTGAACTTC+AGG + chr4.4:83382630-83382649 None:intergenic 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG + chr4.4:83380820-83380839 None:intergenic 50.0%
AGGGTGAGACTCTAGCCACT+TGG - chr4.4:83381570-83381589 MS.gene89795:CDS 55.0%
CCCACCACCATGTCAGAGTA+AGG - chr4.4:83381977-83381996 MS.gene89795:intron 55.0%
GGACAACGCATAGCCCTAGT+TGG - chr4.4:83381511-83381530 MS.gene89795:CDS 55.0%
GGTGGCTAAAAGGCGAACGA+TGG + chr4.4:83380840-83380859 None:intergenic 55.0%
TGCCTTGCACCAGTGACAAC+AGG - chr4.4:83382346-83382365 MS.gene89795:intron 55.0%
! CTTCAGGAATGTTGACGGGC+TGG + chr4.4:83382614-83382633 None:intergenic 55.0%
! TTCGCCTTTTAGCCACCACC+AGG - chr4.4:83380843-83380862 MS.gene89795:intron 55.0%
! AGGCGAACGATGGTGCCAAG+TGG + chr4.4:83380830-83380849 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 83380692 83383183 83380692 ID=MS.gene89795
chr4.4 mRNA 83380692 83383183 83380692 ID=MS.gene89795.t1;Parent=MS.gene89795
chr4.4 exon 83382808 83383183 83382808 ID=MS.gene89795.t1.exon1;Parent=MS.gene89795.t1
chr4.4 CDS 83382808 83383183 83382808 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
chr4.4 exon 83382518 83382564 83382518 ID=MS.gene89795.t1.exon2;Parent=MS.gene89795.t1
chr4.4 CDS 83382518 83382564 83382518 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
chr4.4 exon 83381482 83381650 83381482 ID=MS.gene89795.t1.exon3;Parent=MS.gene89795.t1
chr4.4 CDS 83381482 83381650 83381482 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
chr4.4 exon 83381212 83381397 83381212 ID=MS.gene89795.t1.exon4;Parent=MS.gene89795.t1
chr4.4 CDS 83381212 83381397 83381212 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
chr4.4 exon 83380894 83381171 83380894 ID=MS.gene89795.t1.exon5;Parent=MS.gene89795.t1
chr4.4 CDS 83380894 83381171 83380894 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
chr4.4 exon 83380692 83380817 83380692 ID=MS.gene89795.t1.exon6;Parent=MS.gene89795.t1
chr4.4 CDS 83380692 83380817 83380692 ID=cds.MS.gene89795.t1;Parent=MS.gene89795.t1
Gene Sequence

>MS.gene89795

ATGGCTTCTGAACAGCTACAAAAAGATAAAAGAACAATTCCCCTCAAAATGTTAGTGGACAAACAAAGGAACAAAGTTGTTTTTGTGGAAGCAACCAAGGACTTTGTTGACACTCTCTTCAGCTTCCTATCTCTTCCACTTGGCACCATCGTTCGCCTTTTAGCCACCACCAGGAATAACAACAACAATGACCAACAATCAGAATCAATACCATTCCTTGACAACATCAGAAACCTCTACCGTACCGTCCAAAATCTCAATTCAAATGATGTTTGGAACAATGCTGTCTGCAAACAAATGCTGTTGCATCCCAGAAATCCATGTGAAGCATTGTTCATGAAGCTGTTCTTGAATGTTGATGACACTGAGCCATCAAGTAAGTTTTTTGTGTGTGATACTTGCTACAAGTTTACTACTTTTCAGAATCTTGATTGCACCTGTGGAAAACCCACTAATAGATAGCCTAAGAACTTGGATTCTGAGGGTCATGAGAGTAATGCCCAAAATGAGGTCTTTATAAGAGAAAATGGATCAATGTTTCTGGTGTCTGATGATTTGAAGATTATGCCAAGTTCCTCTGTGACTTACTTGAAGCTGTTGATGGAATTGGGGTATTCAGATTTGGCTCAAATAGAGGAAGTCACACACAACATTGGCAAACAAGAGGTAATGCTTTTTGTGTTTGATCTGTATCTATCTCACAATTATCAGCTCCCATTACATTGAATTTGTTAATTAATATTCACAATCATTTGGATGTCATTTTTTACAAGCAAAAGAAAATATATTAAATATCTGTGATCACAACTTAGTTGGTAGGGACAACGCATAGCCCTAGTTGGTGTCTGAACCTTGAATTTACTGCTTATTCACCTTAAAGGGTGAGACTCTAGCCACTTGGCTAATTGACAAAAATAAAGAAATACTATGGGCAGTAGTACACTAGTACTTCAACCCAATACAAGAAAAAGCAATACTATGGGCATGTTTCGATTAACTTTTAGTTGAAATCTAAAGACATAAGCACCGATAAGAATGTTTGGTAAATGTTATGAAAGTAGCTTATGACAAATCTATAAGAATGTTTTCCATACGACTATTATTTGATATTTTATAATGGATTAATTTGAGAGTATACAAAGTCTGCGAGAGTATAAGCATGTTTGAGTCAACTAAAAAAATCGCATCTAAGCACAAGGTGTGCATTGTGCAAGGATGGCACAGTATACGAAGTAATATTCAATAAGAGCATTAAAACTCAAAAAATATCAATACTAAAGATTAACCCACCACCATGTCAGAGTAAGGTCACTAGGTTTGGGCCTAAGATGAGAAGATAAGATCGTCCTGCATTTTTCTGTTAGAAAACATGTGGTTTCAGCTGTGACTGAAACATCTCTTCTTAGAGGAACTTCCACTCCAGGATTACGATGGATTTGCTTCTTTAATAGATCTATTTTCAATAAATAATATAATTTATTCTTCTTTGTTGTATTGTTATGCTTATTATTTGTGTTTTAACATATTCTATAGATATTGAACCTCCTCAAGCATACCTTAACCTCTCATGAGCCTTTGACAAATACATTTTTTGCAAGTAGCTCCAAGAACAAAGACAAACGACCTAACCAACTTGCATCAGCAGTTAGAGCAATGCCTTGCACCAGTGACAACAGGAAATTGGATGTTAAAGTAATTCAAAGTAAATCTCAAAAAAATATAATCTTTGCAGAAACCAATGGAGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTAGGCTCCATTGTAAAGCTTTTGGGAACTAACTCTTTTGATGGGTGTGTTGGTAATTTGTACAATAGTGTGAAGAAGTTGGACCCCAAGTCTGTGTTACTCGATCCTGGTATAGCACACCTGTTTAGTTGTCTTAACCAGCCCGTCAACATTCCTGAAGTTCAACCACAACCCACTACATATTACTATGGTACAGGGACAAAAAAAAAAAGTGTATTCGCGTCACATAGAGTGTGATATAACAGTAGAAGTAAAGATAGAGGGAGGAGTGATTTCTAAGTCACAGGGATCACTTTATCAAGCGAAACCTTTGATTGAACTGGACCCTAGATCCCTCAACAGAACAGAAGGTGTTATTGGATTCGTGAAGAGAGTATCATTATATGGTATAGGAGATGATCTAAGAGTAAAACCACTTTCTGCTAATTGTTTCCTTTCATATTTGAAAAAATTAAGCCATCCTCTTGATGATCTTCAAGTGAAGGTGATAAGCATTGGTGAAGCTGAGGTAATGTGGCTATGATTTACCACTAAATATATTTTTGTTATTTTAGCCTTTTTGATTCACTTTAATTGTGGTGTAGGCTTTGAACTTCCTTGGAGCATTCTTGACATCAAGATTTACCTTGACGAGTGGACTTAAAGACCTTTTGAGTGTGCCTAAACCAGAATCAATTTTTTCAAAGTTCCTAAAAAGGAATGAACATTGA
Protein sequence

>MS.gene89795.t1

MASEQLQKDKRTIPLKMLVDKQRNKVVFVEATKDFVDTLFSFLSLPLGTIVRLLATTRNNNNNDQQSESIPFLDNIRNLYRTVQNLNSNDVWNNAVCKQMLLHPRNPCEALFMKLFLNVDDTEPSNLAQIEEVTHNIGKQEILNLLKHTLTSHEPLTNTFFASSSKNKDKRPNQLASAVRAMPCTSDNRKLDVKVIQSSIVKLLGTNSFDGCVGNLYNSVKKLDPKSVLLDPGIAHLFSCLNQPVNIPEVQPQPTTYYYECDITVEVKIEGGVISKSQGSLYQAKPLIELDPRSLNRTEGVIGFVKRVSLYGIGDDLRVKPLSANCFLSYLKKLSHPLDDLQVKVISIGEAEALNFLGAFLTSRFTLTSGLKDLLSVPKPESIFSKFLKRNEH