AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037550

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037550.t1 MTR_4g017410 68.315 445 101 6 4 439 75 488 0.0 579
MS.gene037550.t1 MTR_4g017440 64.126 446 133 5 4 441 82 508 0.0 555
MS.gene037550.t1 MTR_4g017550 67.126 435 117 8 4 431 54 469 0.0 547
MS.gene037550.t1 MTR_4g017830 62.277 448 141 7 4 439 74 505 0.0 539
MS.gene037550.t1 MTR_4g017830 30.037 273 134 10 180 431 2 238 5.54e-15 78.6
MS.gene037550.t1 MTR_4g017470 60.360 444 120 7 4 439 79 474 1.72e-168 490
MS.gene037550.t1 MTR_4g019340 61.556 437 112 9 4 424 106 502 7.83e-168 489
MS.gene037550.t1 MTR_4g017610 57.506 433 147 7 4 432 77 476 2.83e-160 468
MS.gene037550.t1 MTR_4g019310 40.000 445 217 12 5 431 74 486 9.99e-89 285
MS.gene037550.t1 MTR_4g019310 34.615 104 54 2 190 280 3 105 3.58e-12 69.7
MS.gene037550.t1 MTR_7g058710 31.707 451 247 10 6 431 59 473 4.75e-51 184
MS.gene037550.t1 MTR_7g066570 33.115 459 239 16 6 441 73 486 1.48e-50 184
MS.gene037550.t1 MTR_7g066350 31.839 446 216 12 6 431 68 445 4.11e-50 181
MS.gene037550.t1 MTR_7g066350 29.200 250 133 6 194 429 8 227 2.50e-14 76.3
MS.gene037550.t1 MTR_7g066400 31.264 435 234 13 6 431 55 433 8.05e-47 171
MS.gene037550.t1 MTR_7g066430 30.526 475 269 14 6 458 63 498 8.68e-45 167
MS.gene037550.t1 MTR_7g066410 30.835 467 255 13 6 431 69 508 2.97e-44 166
MS.gene037550.t1 MTR_7g062280 31.681 464 234 14 6 434 69 484 2.78e-43 162
MS.gene037550.t1 MTR_7g066340 34.122 296 169 8 6 282 71 359 1.25e-42 160
MS.gene037550.t1 MTR_7g066340 28.226 248 128 7 195 424 12 227 1.49e-15 80.1
MS.gene037550.t1 MTR_7g063900 30.752 452 251 13 6 434 69 481 1.61e-42 160
MS.gene037550.t1 MTR_7g063900 27.652 264 141 8 195 443 10 238 3.12e-12 69.7
MS.gene037550.t1 MTR_7g063660 31.136 440 255 14 6 434 68 470 5.60e-42 159
MS.gene037550.t1 MTR_7g063660 36.207 116 61 2 190 292 4 119 4.93e-14 75.5
MS.gene037550.t1 MTR_7g066260 30.702 456 215 17 6 431 74 458 1.45e-40 155
MS.gene037550.t1 MTR_7g066260 30.682 264 139 10 195 443 15 249 5.80e-15 78.2
MS.gene037550.t1 MTR_7g062300 30.752 452 240 15 6 434 71 472 1.51e-39 152
MS.gene037550.t1 MTR_7g062300 32.353 136 69 4 190 302 7 142 7.15e-11 65.5
MS.gene037550.t1 MTR_7g012680 30.303 462 213 15 6 441 73 451 3.92e-38 147
MS.gene037550.t1 MTR_7g063880 29.438 445 245 15 6 434 70 461 2.76e-37 145
MS.gene037550.t1 MTR_7g063880 35.294 119 64 2 190 295 6 124 9.40e-14 74.3
MS.gene037550.t1 MTR_7g063890 29.535 430 234 13 6 409 86 472 1.44e-36 144
MS.gene037550.t1 MTR_7g063890 26.877 253 138 8 191 429 23 242 1.03e-11 68.2
MS.gene037550.t1 MTR_7g066580 29.018 448 242 13 6 436 54 442 2.43e-35 139
MS.gene037550.t1 MTR_3g065200 27.083 432 241 9 6 430 52 416 2.75e-34 136
MS.gene037550.t1 MTR_1g041315 32.198 323 181 12 6 296 73 389 1.19e-33 135
MS.gene037550.t1 MTR_7g063840 28.665 457 239 14 6 434 69 466 1.88e-33 134
MS.gene037550.t1 MTR_7g058700 34.629 283 122 9 26 272 583 838 7.40e-33 135
MS.gene037550.t1 MTR_7g063830 28.222 450 221 13 6 434 69 437 5.22e-32 129
MS.gene037550.t1 MTR_7g063920 29.025 441 220 15 6 434 70 429 2.89e-31 127
MS.gene037550.t1 MTR_7g066360 35.172 290 162 11 6 280 73 351 2.00e-30 125
MS.gene037550.t1 MTR_7g066360 26.804 291 158 9 196 472 15 264 1.08e-11 67.8
MS.gene037550.t1 MTR_7g063820 30.617 454 245 17 6 434 69 477 8.33e-30 123
MS.gene037550.t1 MTR_7g066290 34.014 294 169 8 6 280 74 361 1.66e-29 122
MS.gene037550.t1 MTR_7g066290 44.928 69 33 1 194 257 14 82 9.86e-11 64.7
MS.gene037550.t1 MTR_3g007740 27.500 440 242 12 6 434 68 441 2.21e-27 115
MS.gene037550.t1 MTR_7g066480 33.091 275 139 9 6 258 75 326 2.98e-27 115
MS.gene037550.t1 MTR_7g063670 26.291 426 243 12 25 434 80 450 4.82e-25 108
MS.gene037550.t1 MTR_7g066280 31.092 238 129 6 27 256 1 211 6.13e-25 108
MS.gene037550.t1 MTR_7g066310 27.797 295 124 8 6 282 66 289 2.75e-22 100
MS.gene037550.t1 MTR_7g066680 30.000 280 157 7 146 415 37 287 4.93e-22 98.2
MS.gene037550.t1 MTR_7g063720 29.771 262 126 6 185 434 10 225 8.01e-22 95.5
MS.gene037550.t1 MTR_8g445860 28.253 269 112 9 194 429 12 232 1.39e-18 85.9
MS.gene037550.t1 MTR_7g066490 27.925 265 150 7 6 257 75 311 5.37e-18 87.0
MS.gene037550.t1 MTR_7g100420 29.429 350 181 14 98 431 94 393 3.88e-17 84.3
MS.gene037550.t1 MTR_1g115440 26.190 252 142 6 194 429 10 233 2.36e-15 76.6
MS.gene037550.t1 MTR_1g115450 42.500 80 38 1 194 265 6 85 5.80e-14 69.7
MS.gene037550.t1 MTR_1g115455 39.080 87 45 1 187 265 1 87 1.74e-13 70.9
MS.gene037550.t1 MTR_8g445920 41.791 67 39 0 190 256 5 71 8.10e-13 65.5
MS.gene037550.t1 MTR_8g044890 41.791 67 39 0 190 256 5 71 8.10e-13 65.5
MS.gene037550.t1 MTR_0150s0030 38.372 86 43 2 194 270 7 91 2.41e-12 67.0
MS.gene037550.t1 MTR_1g014060 32.121 165 95 7 6 161 59 215 2.43e-12 69.7
MS.gene037550.t1 MTR_8g445910 39.535 86 42 2 194 270 7 91 9.54e-12 66.2
MS.gene037550.t1 MTR_1g115470 41.250 80 39 1 194 265 5 84 1.00e-11 67.8
MS.gene037550.t1 MTR_8g044880 39.506 81 40 1 194 265 7 87 1.69e-11 65.9
MS.gene037550.t1 MTR_2g029400 38.835 103 50 2 195 284 14 116 2.14e-11 66.6
MS.gene037550.t1 MTR_4g019330 47.500 80 40 2 362 439 107 186 2.19e-11 63.5
MS.gene037550.t1 MTR_0150s0010 39.286 84 43 1 190 265 5 88 2.39e-11 64.7
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037550.t1 AT5G01140 30.753 465 241 18 4 442 50 459 4.25e-44 164
MS.gene037550.t1 AT5G01130 29.841 439 237 13 5 434 67 443 3.59e-42 159
MS.gene037550.t1 AT5G01120 29.299 471 273 17 5 450 67 502 4.74e-39 151
MS.gene037550.t1 AT5G01120 28.667 300 160 12 194 473 8 273 8.02e-14 74.7
MS.gene037550.t1 AT5G43240 29.130 460 256 16 5 431 67 489 1.51e-33 135
MS.gene037550.t1 AT5G43240 29.167 216 111 8 196 394 10 200 8.89e-13 71.6
MS.gene037550.t1 AT5G43240 29.130 460 256 16 5 431 67 489 1.51e-33 135
MS.gene037550.t1 AT5G43240 29.167 216 111 8 196 394 10 200 8.89e-13 71.6
MS.gene037550.t1 AT5G43240 29.130 460 256 16 5 431 67 489 1.51e-33 135
MS.gene037550.t1 AT5G43240 29.167 216 111 8 196 394 10 200 8.89e-13 71.6
MS.gene037550.t1 AT3G09140 26.638 473 257 15 5 434 67 492 2.04e-32 131
MS.gene037550.t1 AT3G09140 27.823 248 152 6 195 428 9 243 9.98e-16 80.9
MS.gene037550.t1 AT5G01150 27.617 449 269 13 5 434 67 478 1.13e-31 129
MS.gene037550.t1 AT3G09110 33.588 262 154 8 5 256 67 318 6.95e-30 121
MS.gene037550.t1 AT3G09140 25.150 501 257 16 5 434 67 520 1.25e-27 117
MS.gene037550.t1 AT3G09140 26.761 213 130 5 195 394 9 208 1.29e-12 71.2
MS.gene037550.t1 AT5G37320 27.033 455 263 15 5 434 68 478 7.91e-27 114
MS.gene037550.t1 AT3G09120 30.224 268 149 9 4 249 62 313 8.98e-26 108
MS.gene037550.t1 AT3G09120 27.419 248 143 6 195 429 6 229 3.36e-16 80.5
MS.gene037550.t1 AT5G43240 29.395 347 192 10 104 431 33 345 4.57e-25 107

Find 155 sgRNAs with CRISPR-Local

Find 223 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAAACTTTGGTCCATTT+TGG 0.099278 4.3:-81960131 None:intergenic
GCCACCTTTGGCACGCTATT+TGG 0.164828 4.3:+81962107 MS.gene037550:CDS
AAACCAACAGCCTCGAAATT+TGG 0.181777 4.3:+81960071 MS.gene037550:CDS
GCCTCTCACAAATACAATTT+TGG 0.184722 4.3:+81960931 MS.gene037550:CDS
GGACCTCCTTCCGTCAATTT+TGG 0.186758 4.3:+81962038 MS.gene037550:CDS
GAACACCCTGCTTATTATTA+TGG 0.198395 4.3:+81961304 MS.gene037550:CDS
CCTTGGTTCCAGTGCAATTA+AGG 0.221659 4.3:-81961488 None:intergenic
AGGATCCATTGTAAAGCTTT+TGG 0.229184 4.3:+81961144 MS.gene037550:CDS
CTTGCAGCTGTTGATGGAAT+TGG 0.234153 4.3:+81960197 MS.gene037550:CDS
TTCTTTACTCGGCGTGATAT+CGG 0.236275 4.3:+81962067 MS.gene037550:CDS
CCAAAATGGACCAAAGTTTC+TGG 0.249363 4.3:+81960131 MS.gene037550:CDS
TGTTGATCCCTCTTTCTCTT+TGG 0.267814 4.3:-81961337 None:intergenic
GCAGCTTCTATACCGTCTTT+TGG 0.269844 4.3:+81962329 MS.gene037550:CDS
ACTACTCCTACGAATTCTTT+TGG 0.270872 4.3:+81962143 MS.gene037550:CDS
GCAAAGTAAATCTCAGAAAA+AGG 0.300596 4.3:+81961057 MS.gene037550:CDS
AAAGGTGGCGGAACCGAAAA+TGG 0.307297 4.3:-81962093 None:intergenic
TCATCATCCACCTCCGCATT+TGG 0.313927 4.3:+81961939 MS.gene037550:CDS
TCTTCTGGTACACCGTCTTT+TGG 0.322220 4.3:+81962299 MS.gene037550:CDS
TCGGCTTCAAATACAGCTTC+AGG 0.323250 4.3:+81962173 MS.gene037550:CDS
AATATTGCGGGTGCCCAAAA+TGG 0.336180 4.3:+81960117 MS.gene037550:CDS
GACTTACTTGCAGCTGTTGA+TGG 0.338778 4.3:+81960191 MS.gene037550:CDS
CAGTGATTCACATGGATTCC+TGG 0.341087 4.3:-81959929 None:intergenic
CCGTTTGGAGTACCGTCCTT+TGG 0.341591 4.3:+81962230 MS.gene037550:CDS
GGATTGAGTAACACAGAAAC+AGG 0.345671 4.3:-81961226 None:intergenic
ACCTACACTCAGACGATGTT+TGG 0.345816 4.3:+81959872 MS.gene037550:CDS
AAGGCTTCTACACCATTGTT+TGG 0.357666 4.3:+81962389 MS.gene037550:CDS
TCGGCTTCTACACCATTGTT+TGG 0.359582 4.3:+81962263 MS.gene037550:CDS
TGAATGTTCGATTGCAAAAC+GGG 0.360175 4.3:-81961866 None:intergenic
TTGCGCCGTCGTTTGGAGTT+TGG 0.360350 4.3:+81962426 MS.gene037550:CDS
AGTTTCCTTACCATGCCATT+AGG 0.365207 4.3:+81961124 MS.gene037550:CDS
TTGCAGCTGTTGATGGAATT+GGG 0.365599 4.3:+81960198 MS.gene037550:CDS
TCAGATTTCGCGGAGTATAA+TGG 0.378322 4.3:+81961364 MS.gene037550:CDS
GTGATAGAAGAAAAGATAGA+AGG 0.378763 4.3:+81961421 MS.gene037550:CDS
GGAGCACCATCATCATCTTC+TGG 0.379469 4.3:+81962284 MS.gene037550:CDS
TTTCGAGGCTGTTGGTTTGT+TGG 0.381093 4.3:-81960066 None:intergenic
GTGTAGGCTTTGAACTTCCT+TGG 0.395429 4.3:+81961771 MS.gene037550:intron
CATCGTCTGAGTGTAGGTTT+TGG 0.399061 4.3:-81959867 None:intergenic
ACAGCTTCAGGGTCGTCGTT+TGG 0.405842 4.3:+81962185 MS.gene037550:CDS
ACGGCTTCTACACCGTCGTT+TGG 0.416764 4.3:+81962359 MS.gene037550:CDS
CAGAAGTAAAATTAATGTTA+AGG 0.418763 4.3:+81961030 MS.gene037550:CDS
GTTTCTGTGTTACTCAATCC+TGG 0.427223 4.3:+81961229 MS.gene037550:CDS
TGCGAAGTCCTTAATTGCAC+TGG 0.430913 4.3:+81961480 MS.gene037550:CDS
CAAGAGAATAGTAATATTGC+GGG 0.432706 4.3:+81960105 MS.gene037550:CDS
CAAAACCTTCATTGTACTTG+TGG 0.434397 4.3:+81960039 MS.gene037550:CDS
ATTTGTGAGAGGCTCATTAG+AGG 0.437734 4.3:-81960921 None:intergenic
AAAGAATTCGTAGGAGTAGT+AGG 0.438431 4.3:-81962140 None:intergenic
CCTGGTATAGCACCACAGTT+TGG 0.441678 4.3:+81961247 MS.gene037550:CDS
GAGAGGCTCATTAGAGGTTA+AGG 0.444319 4.3:-81960915 None:intergenic
CCGACATCTTCAACTCCGTT+TGG 0.451844 4.3:+81962215 MS.gene037550:CDS
AACAAATGTTGCTGCATCCC+AGG 0.457734 4.3:+81959911 MS.gene037550:CDS
AACGACGGCGCAAAAGCCGC+TGG 0.458595 4.3:-81962416 None:intergenic
ACTGTTGATGCAGATTGCTT+AGG 0.463635 4.3:-81960983 None:intergenic
TTCAGATGCGACTCAATTAG+AGG 0.463812 4.3:+81960224 MS.gene037550:CDS
TCATCAGACACCAGAAACTT+TGG 0.465061 4.3:-81960141 None:intergenic
TCCTCAATTTCAAAACCAAA+AGG 0.467135 4.3:+81961896 MS.gene037550:CDS
TTTGGATCCTCTTCTTTACT+CGG 0.473778 4.3:+81962056 MS.gene037550:CDS
GGAAGGAGATGGCAAGAAAG+AGG 0.475700 4.3:-81962006 None:intergenic
CCTGTACCATAATAATAAGC+AGG 0.479447 4.3:-81961310 None:intergenic
CCTGCTTATTATTATGGTAC+AGG 0.483192 4.3:+81961310 MS.gene037550:CDS
GGAATGTTGAGTGGTTGGTT+TGG 0.486563 4.3:-81961274 None:intergenic
TTGAATGTTCGATTGCAAAA+CGG 0.488665 4.3:-81961867 None:intergenic
TAATGGTAATCGTATAATGA+TGG 0.489399 4.3:+81961381 MS.gene037550:CDS
TGGGATTTGTGAAGAGAGAA+GGG 0.491787 4.3:+81961539 MS.gene037550:CDS
AGCTTTACAATGGATCCTAA+TGG 0.496722 4.3:-81961139 None:intergenic
CCAAACTGTGGTGCTATACC+AGG 0.500141 4.3:-81961247 None:intergenic
AGGAGAAGAAGTTGTGGCAG+AGG 0.501885 4.3:-81961976 None:intergenic
ATTAACCTTGACTAGTGGAC+TGG 0.503661 4.3:+81961812 MS.gene037550:CDS
ACCTTTGGCACGCTATTTGG+TGG 0.508457 4.3:+81962110 MS.gene037550:CDS
CACACACAACATTGGCAAGC+AGG 0.511161 4.3:+81960251 MS.gene037550:CDS
CAGGAACACCAAAGAGAAAG+AGG 0.520391 4.3:+81961329 MS.gene037550:CDS
GCCAAAATTGTATTTGTGAG+AGG 0.523537 4.3:-81960932 None:intergenic
CCAAAGGACGGTACTCCAAA+CGG 0.525259 4.3:-81962230 None:intergenic
AGAGAAGGGTTATATTGTGT+AGG 0.527009 4.3:+81961553 MS.gene037550:CDS
CGGCTTCAAATACAGCTTCA+GGG 0.527253 4.3:+81962174 MS.gene037550:CDS
TTTCAAGATCATCAAGAGGA+AGG 0.531048 4.3:-81961639 None:intergenic
CCATCTCCTTCCTCTTCTAG+AGG 0.534956 4.3:+81962017 MS.gene037550:CDS
TTACTTCTGTTATTGGTGCA+AGG 0.535137 4.3:-81961016 None:intergenic
ACGACGGCGCAAAAGCCGCT+GGG 0.536665 4.3:-81962415 None:intergenic
CCTTAATTGCACTGGAACCA+AGG 0.537318 4.3:+81961488 MS.gene037550:CDS
GTAACACAGAAACAGGGTTA+AGG 0.537772 4.3:-81961219 None:intergenic
AGTGATTCACATGGATTCCT+GGG 0.538053 4.3:-81959928 None:intergenic
AATCCAAATTTCGAGGCTGT+TGG 0.539114 4.3:-81960074 None:intergenic
TCTGAGGAATGTTGAGTGGT+TGG 0.545273 4.3:-81961279 None:intergenic
AGCCGATGACGATGCTCCAA+AGG 0.545629 4.3:-81962246 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.547627 4.3:+81961192 MS.gene037550:CDS
TCAACATTCCTCAGATTTCG+CGG 0.550964 4.3:+81961354 MS.gene037550:CDS
TGGATCCTAATGGCATGGTA+AGG 0.551509 4.3:-81961129 None:intergenic
ATAGAAGAAAAGATAGAAGG+AGG 0.551511 4.3:+81961424 MS.gene037550:CDS
GTCCTTTGGAGCATCGTCAT+CGG 0.554103 4.3:+81962244 MS.gene037550:CDS
AATGCGGAGGTGGATGATGA+AGG 0.554362 4.3:-81961936 None:intergenic
AGATGCGAATGAACAGAAAG+TGG 0.554908 4.3:-81961595 None:intergenic
AAATAGCGTGCCAAAGGTGG+CGG 0.556331 4.3:-81962105 None:intergenic
TGAGGATCCAAATGCGGAGG+TGG 0.558954 4.3:-81961946 None:intergenic
GTGGGATTTGTGAAGAGAGA+AGG 0.565305 4.3:+81961538 MS.gene037550:CDS
CCGTACGAATCCAAATTTCG+AGG 0.565407 4.3:-81960081 None:intergenic
GCCGTTGGAGCTCCAAAAGA+CGG 0.565551 4.3:-81962341 None:intergenic
ACCAAATAGCGTGCCAAAGG+TGG 0.567039 4.3:-81962108 None:intergenic
GGCAGAAGGAGAAGAAGTTG+TGG 0.572188 4.3:-81961982 None:intergenic
CGGAGGTGGATGATGAAGGA+GGG 0.572769 4.3:-81961932 None:intergenic
TCAAGAGAATAGTAATATTG+CGG 0.576099 4.3:+81960104 MS.gene037550:CDS
TTCATTTCAAGATCATCAAG+AGG 0.577676 4.3:-81961643 None:intergenic
AGAAGAGGATCCAAAATTGA+CGG 0.578525 4.3:-81962048 None:intergenic
TCAAAATTAACCTTGACTAG+TGG 0.580631 4.3:+81961807 MS.gene037550:CDS
CCTCGAAATTTGGATTCGTA+CGG 0.581423 4.3:+81960081 MS.gene037550:CDS
GCGGAGGTGGATGATGAAGG+AGG 0.581895 4.3:-81961933 None:intergenic
TACAATGGATCCTAATGGCA+TGG 0.582946 4.3:-81961134 None:intergenic
GATAAGCATTGGTGAAGTTG+AGG 0.584497 4.3:+81961669 MS.gene037550:CDS
AGATCCCAAAAGCTTTACAA+TGG 0.585059 4.3:-81961149 None:intergenic
GGCAAGAAAGAGGAGGCAGA+AGG 0.585799 4.3:-81961996 None:intergenic
GATCCAAAATTGACGGAAGG+AGG 0.589093 4.3:-81962041 None:intergenic
ACCACCAAATAGCGTGCCAA+AGG 0.596309 4.3:-81962111 None:intergenic
GTCTCCCAGTCCACTAGTCA+AGG 0.597674 4.3:-81961817 None:intergenic
GCCTTTGATGTTCCAAACGA+CGG 0.598640 4.3:-81962371 None:intergenic
CCTCTAGAAGAGGAAGGAGA+TGG 0.603237 4.3:-81962017 None:intergenic
CGACGGCGCAAAAGCCGCTG+GGG 0.604534 4.3:-81962414 None:intergenic
GCTGCCGGTGCTCCAAAAGA+CGG 0.605110 4.3:-81962311 None:intergenic
GAAGTCAACAAAATCACCGT+TGG 0.609986 4.3:-81961095 None:intergenic
ATTATACTCCGCGAAATCTG+AGG 0.611140 4.3:-81961362 None:intergenic
AACGACGGTGTAGAAGCCGT+TGG 0.611925 4.3:-81962356 None:intergenic
GGAAGGAGGTCCTCTAGAAG+AGG 0.613771 4.3:-81962027 None:intergenic
GGAGGTCCTCTAGAAGAGGA+AGG 0.615174 4.3:-81962023 None:intergenic
CGTATAATGATGGATTGTAG+TGG 0.615640 4.3:+81961391 MS.gene037550:CDS
TGCAGCTGTTGATGGAATTG+GGG 0.616569 4.3:+81960199 MS.gene037550:CDS
ACCGTCGTTTGGAACATCAA+AGG 0.616749 4.3:+81962370 MS.gene037550:CDS
AAGTAAGTCACTGACGAACA+TGG 0.618986 4.3:-81960177 None:intergenic
TTAACCTTGACTAGTGGACT+GGG 0.619919 4.3:+81961813 MS.gene037550:CDS
GAAATGAAGCTGATAAGCAT+TGG 0.620330 4.3:+81961658 MS.gene037550:CDS
GAGGAAGTCACACACAACAT+TGG 0.621785 4.3:+81960243 MS.gene037550:CDS
TAAGCAGGGTGTTCAATCTG+AGG 0.622346 4.3:-81961295 None:intergenic
GAGGATCCAAAATTGACGGA+AGG 0.631203 4.3:-81962044 None:intergenic
AAAGACGGTATAGAAGCTGC+CGG 0.631499 4.3:-81962326 None:intergenic
GTGATATATGCAGAAGCCAA+CGG 0.632346 4.3:+81961079 MS.gene037550:CDS
CTGTACCATAATAATAAGCA+GGG 0.633705 4.3:-81961309 None:intergenic
TATCACGCCGAGTAAAGAAG+AGG 0.639003 4.3:-81962063 None:intergenic
AGGAGATGGCAAGAAAGAGG+AGG 0.640462 4.3:-81962003 None:intergenic
TCCAAACATCGTCTGAGTGT+AGG 0.640477 4.3:-81959873 None:intergenic
TTGATGTCAAGAATGCTCCA+AGG 0.651294 4.3:-81961788 None:intergenic
GCCGCTGGGGATCCAAACAA+TGG 0.651558 4.3:-81962401 None:intergenic
CCAAACGGAGTTGAAGATGT+CGG 0.662695 4.3:-81962215 None:intergenic
GTTGCCCCAAAAGAATTCGT+AGG 0.667496 4.3:-81962149 None:intergenic
ACCATTGTTTGGATCCCCAG+CGG 0.670336 4.3:+81962400 MS.gene037550:CDS
TCAATCTGAGGAATGTTGAG+TGG 0.670562 4.3:-81961283 None:intergenic
AGGAACACCAAAGAGAAAGA+GGG 0.671429 4.3:+81961330 MS.gene037550:CDS
TTCATGCACAGTGATTCACA+TGG 0.677672 4.3:-81959937 None:intergenic
ACACAACATTGGCAAGCAGG+AGG 0.678135 4.3:+81960254 MS.gene037550:CDS
GATGACGATGCTCCAAAGGA+CGG 0.678623 4.3:-81962242 None:intergenic
TGTGGCAGAGGTTGTCTGTG+AGG 0.679227 4.3:-81961964 None:intergenic
CAAAACCAAACTCCAAACGA+CGG 0.692161 4.3:-81962431 None:intergenic
GATGATGGTGCTCCAAACAA+TGG 0.693801 4.3:-81962275 None:intergenic
GGTGTACCAGAAGATGATGA+TGG 0.698145 4.3:-81962290 None:intergenic
CTGTGAGGATCCAAATGCGG+AGG 0.699470 4.3:-81961949 None:intergenic
TAATTTGTACAAGAGTGTGG+AGG 0.710790 4.3:+81961195 MS.gene037550:CDS
TGGTTTGGACAACCAAACTG+TGG 0.720651 4.3:-81961259 None:intergenic
TGTCTGTGAGGATCCAAATG+CGG 0.722055 4.3:-81961952 None:intergenic
GATTGAGTAACACAGAAACA+GGG 0.732239 4.3:-81961225 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTAATTTTACTTCTGTTAT+TGG - chr4.3:81961026-81961045 None:intergenic 15.0%
!!! TCTAAATTTTACCATAATAA+AGG - chr4.3:81960437-81960456 None:intergenic 15.0%
!!! TTTAAGATCTATTGAATTTT+CGG - chr4.3:81960603-81960622 None:intergenic 15.0%
!! CAGAAGTAAAATTAATGTTA+AGG + chr4.3:81961030-81961049 MS.gene037550:CDS 20.0%
!! TAATTAATATTCACAATCTC+AGG + chr4.3:81960683-81960702 MS.gene037550:intron 20.0%
!! TTTATTGAGAACCTTTATTA+TGG + chr4.3:81960423-81960442 MS.gene037550:intron 20.0%
!!! TCACAAATATATGATTTTAG+AGG - chr4.3:81960501-81960520 None:intergenic 20.0%
!!! TTAAGATCTATTGAATTTTC+GGG - chr4.3:81960602-81960621 None:intergenic 20.0%
! TAATGGTAATCGTATAATGA+TGG + chr4.3:81961381-81961400 MS.gene037550:CDS 25.0%
! TCAAGAGAATAGTAATATTG+CGG + chr4.3:81960104-81960123 MS.gene037550:CDS 25.0%
! TCAATATCTGTACATCAAAA+AGG - chr4.3:81960880-81960899 None:intergenic 25.0%
!!! AGGTAAGTTGTCTTTATTTT+TGG - chr4.3:81960966-81960985 None:intergenic 25.0%
AACCACAAATTAAAACCATG+AGG - chr4.3:81960400-81960419 None:intergenic 30.0%
AGGTAATTAATTCTGTTGTG+TGG + chr4.3:81960274-81960293 MS.gene037550:intron 30.0%
ATAGAAGAAAAGATAGAAGG+AGG + chr4.3:81961424-81961443 MS.gene037550:CDS 30.0%
CAAGAGAATAGTAATATTGC+GGG + chr4.3:81960105-81960124 MS.gene037550:CDS 30.0%
CTGTACCATAATAATAAGCA+GGG - chr4.3:81961312-81961331 None:intergenic 30.0%
GCAAAGTAAATCTCAGAAAA+AGG + chr4.3:81961057-81961076 MS.gene037550:CDS 30.0%
GTGATAGAAGAAAAGATAGA+AGG + chr4.3:81961421-81961440 MS.gene037550:CDS 30.0%
TATATTTGTGACTGCAATTG+AGG + chr4.3:81960510-81960529 MS.gene037550:intron 30.0%
TCAAAATTAACCTTGACTAG+TGG + chr4.3:81961807-81961826 MS.gene037550:CDS 30.0%
TCCTCAATTTCAAAACCAAA+AGG + chr4.3:81961896-81961915 MS.gene037550:CDS 30.0%
TGAAGATTCGTGATTAAGAT+TGG - chr4.3:81960377-81960396 None:intergenic 30.0%
TTCATTTCAAGATCATCAAG+AGG - chr4.3:81961646-81961665 None:intergenic 30.0%
TTGAATGTTCGATTGCAAAA+CGG - chr4.3:81961870-81961889 None:intergenic 30.0%
TTGTACCAAGTTTAAAGGTT+CGG + chr4.3:81960647-81960666 MS.gene037550:intron 30.0%
! CACACAAAAAACTTGTTTGA+TGG - chr4.3:81959991-81960010 None:intergenic 30.0%
! TCTTTGATTTCCTTTACTTG+TGG + chr4.3:81961749-81961768 MS.gene037550:intron 30.0%
!!! TCCTTTTGGTTTTGAAATTG+AGG - chr4.3:81961900-81961919 None:intergenic 30.0%
!!! TTGGTTTTGAAATTGAGGAA+GGG - chr4.3:81961895-81961914 None:intergenic 30.0%
!!! TTTGGTTTTGAAATTGAGGA+AGG - chr4.3:81961896-81961915 None:intergenic 30.0%
AAAGAATTCGTAGGAGTAGT+AGG - chr4.3:81962143-81962162 None:intergenic 35.0%
AAGAGTAGAATAGTGCAGTT+AGG + chr4.3:81960744-81960763 MS.gene037550:intron 35.0%
AGAAGAGGATCCAAAATTGA+CGG - chr4.3:81962051-81962070 None:intergenic 35.0%
AGAGAAGGGTTATATTGTGT+AGG + chr4.3:81961553-81961572 MS.gene037550:CDS 35.0%
AGAGTAGAATAGTGCAGTTA+GGG + chr4.3:81960745-81960764 MS.gene037550:intron 35.0%
AGATCCCAAAAGCTTTACAA+TGG - chr4.3:81961152-81961171 None:intergenic 35.0%
AGCTTTACAATGGATCCTAA+TGG - chr4.3:81961142-81961161 None:intergenic 35.0%
AGTACTACTACATTTCCTGA+AGG - chr4.3:81960546-81960565 None:intergenic 35.0%
ATTTCCTTTACTTGTGGTGT+AGG + chr4.3:81961755-81961774 MS.gene037550:intron 35.0%
CAAAACCTTCATTGTACTTG+TGG + chr4.3:81960039-81960058 MS.gene037550:CDS 35.0%
CAGGAAATGTAGTAGTACTA+GGG + chr4.3:81960547-81960566 MS.gene037550:intron 35.0%
CCTGCTTATTATTATGGTAC+AGG + chr4.3:81961310-81961329 MS.gene037550:CDS 35.0%
CCTGTACCATAATAATAAGC+AGG - chr4.3:81961313-81961332 None:intergenic 35.0%
GAAATGAAGCTGATAAGCAT+TGG + chr4.3:81961658-81961677 MS.gene037550:CDS 35.0%
GAACACCCTGCTTATTATTA+TGG + chr4.3:81961304-81961323 MS.gene037550:CDS 35.0%
GATTGAGTAACACAGAAACA+GGG - chr4.3:81961228-81961247 None:intergenic 35.0%
GCCAAAATTGTATTTGTGAG+AGG - chr4.3:81960935-81960954 None:intergenic 35.0%
GGAAGTCAATTAGTTAGATC+AGG + chr4.3:81960766-81960785 MS.gene037550:intron 35.0%
GTACTACTACATTTCCTGAA+GGG - chr4.3:81960545-81960564 None:intergenic 35.0%
TCAGGAAATGTAGTAGTACT+AGG + chr4.3:81960546-81960565 MS.gene037550:intron 35.0%
TCTGTACATCAAAAAGGAAG+TGG - chr4.3:81960874-81960893 None:intergenic 35.0%
TGAATGTTCGATTGCAAAAC+GGG - chr4.3:81961869-81961888 None:intergenic 35.0%
TTACTTCTGTTATTGGTGCA+AGG - chr4.3:81961019-81961038 None:intergenic 35.0%
TTTCAAGATCATCAAGAGGA+AGG - chr4.3:81961642-81961661 None:intergenic 35.0%
TTTGGATCCTCTTCTTTACT+CGG + chr4.3:81962056-81962075 MS.gene037550:CDS 35.0%
! ACTACTCCTACGAATTCTTT+TGG + chr4.3:81962143-81962162 MS.gene037550:CDS 35.0%
! AGATTTTAGGCAGTTTCGAA+AGG - chr4.3:81961842-81961861 None:intergenic 35.0%
! AGGATCCATTGTAAAGCTTT+TGG + chr4.3:81961144-81961163 MS.gene037550:CDS 35.0%
! AGGTCAAAAATAGGTGTTGT+GGG + chr4.3:81961520-81961539 MS.gene037550:CDS 35.0%
! CAGAAACTTTGGTCCATTTT+GGG - chr4.3:81960133-81960152 None:intergenic 35.0%
! CTACTCCTACGAATTCTTTT+GGG + chr4.3:81962144-81962163 MS.gene037550:CDS 35.0%
! GCCTCTCACAAATACAATTT+TGG + chr4.3:81960931-81960950 MS.gene037550:CDS 35.0%
! GGATCCATTGTAAAGCTTTT+GGG + chr4.3:81961145-81961164 MS.gene037550:CDS 35.0%
! GTAGCTTGTACCAAGTTTAA+AGG + chr4.3:81960642-81960661 MS.gene037550:intron 35.0%
! GTTTTCCACAAGTACAATGA+AGG - chr4.3:81960047-81960066 None:intergenic 35.0%
! TAATTTGTACAAGAGTGTGG+AGG + chr4.3:81961195-81961214 MS.gene037550:CDS 35.0%
! TACTCCTACGAATTCTTTTG+GGG + chr4.3:81962145-81962164 MS.gene037550:CDS 35.0%
! TGGTAATTTGTACAAGAGTG+TGG + chr4.3:81961192-81961211 MS.gene037550:CDS 35.0%
!! CAAAACGGGTTTGAGATTTT+AGG - chr4.3:81961855-81961874 None:intergenic 35.0%
!! CGTATAATGATGGATTGTAG+TGG + chr4.3:81961391-81961410 MS.gene037550:CDS 35.0%
!!! AACACCTATTTTTGACCTGT+TGG - chr4.3:81961518-81961537 None:intergenic 35.0%
!!! ACACCTATTTTTGACCTGTT+GGG - chr4.3:81961517-81961536 None:intergenic 35.0%
!!! CACCTCATGGTTTTAATTTG+TGG + chr4.3:81960395-81960414 MS.gene037550:intron 35.0%
!!! GGTGTGAAGTTTTTTCCTTT+TGG - chr4.3:81961914-81961933 None:intergenic 35.0%
AAACCAACAGCCTCGAAATT+TGG + chr4.3:81960071-81960090 MS.gene037550:CDS 40.0%
AAAGCCTACACCACAAGTAA+AGG - chr4.3:81961762-81961781 None:intergenic 40.0%
AAGGCTTCTACACCATTGTT+TGG + chr4.3:81962389-81962408 MS.gene037550:CDS 40.0%
AAGTAAGTCACTGACGAACA+TGG - chr4.3:81960180-81960199 None:intergenic 40.0%
AATCACGAATCTTCACCTCA+TGG + chr4.3:81960382-81960401 MS.gene037550:intron 40.0%
AATCCAAATTTCGAGGCTGT+TGG - chr4.3:81960077-81960096 None:intergenic 40.0%
ACTGTTGATGCAGATTGCTT+AGG - chr4.3:81960986-81961005 None:intergenic 40.0%
AGATGCGAATGAACAGAAAG+TGG - chr4.3:81961598-81961617 None:intergenic 40.0%
AGGAACACCAAAGAGAAAGA+GGG + chr4.3:81961330-81961349 MS.gene037550:CDS 40.0%
AGTGATTCACATGGATTCCT+GGG - chr4.3:81959931-81959950 None:intergenic 40.0%
AGTTTCCTTACCATGCCATT+AGG + chr4.3:81961124-81961143 MS.gene037550:CDS 40.0%
ATGGTGTATCAGTTAGTGTC+GGG + chr4.3:81960574-81960593 MS.gene037550:intron 40.0%
ATTATACTCCGCGAAATCTG+AGG - chr4.3:81961365-81961384 None:intergenic 40.0%
ATTTGTGAGAGGCTCATTAG+AGG - chr4.3:81960924-81960943 None:intergenic 40.0%
CAAAACCAAACTCCAAACGA+CGG - chr4.3:81962434-81962453 None:intergenic 40.0%
CCAAAATGGACCAAAGTTTC+TGG + chr4.3:81960131-81960150 MS.gene037550:CDS 40.0%
CCAAGTTTAAAGGTTCGGAA+AGG + chr4.3:81960652-81960671 MS.gene037550:intron 40.0%
CCTCGAAATTTGGATTCGTA+CGG + chr4.3:81960081-81960100 MS.gene037550:CDS 40.0%
CCTTTCCGAACCTTTAAACT+TGG - chr4.3:81960655-81960674 None:intergenic 40.0%
GAAAAATGCGCTCACAACTT+CGG + chr4.3:81960455-81960474 MS.gene037550:intron 40.0%
GAAGTCAACAAAATCACCGT+TGG - chr4.3:81961098-81961117 None:intergenic 40.0%
GATGGTGTATCAGTTAGTGT+CGG + chr4.3:81960573-81960592 MS.gene037550:intron 40.0%
GGATTGAGTAACACAGAAAC+AGG - chr4.3:81961229-81961248 None:intergenic 40.0%
GTAACACAGAAACAGGGTTA+AGG - chr4.3:81961222-81961241 None:intergenic 40.0%
GTAGTAGTACTAGGGTTTGA+TGG + chr4.3:81960555-81960574 MS.gene037550:intron 40.0%
GTGATATATGCAGAAGCCAA+CGG + chr4.3:81961079-81961098 MS.gene037550:CDS 40.0%
TACAATGGATCCTAATGGCA+TGG - chr4.3:81961137-81961156 None:intergenic 40.0%
TCAACATTCCTCAGATTTCG+CGG + chr4.3:81961354-81961373 MS.gene037550:CDS 40.0%
TCAATCTGAGGAATGTTGAG+TGG - chr4.3:81961286-81961305 None:intergenic 40.0%
TCAGATTTCGCGGAGTATAA+TGG + chr4.3:81961364-81961383 MS.gene037550:CDS 40.0%
TCATCAGACACCAGAAACTT+TGG - chr4.3:81960144-81960163 None:intergenic 40.0%
TGGGATTTGTGAAGAGAGAA+GGG + chr4.3:81961539-81961558 MS.gene037550:CDS 40.0%
TGTTGATCCCTCTTTCTCTT+TGG - chr4.3:81961340-81961359 None:intergenic 40.0%
TTCAGATGCGACTCAATTAG+AGG + chr4.3:81960224-81960243 MS.gene037550:CDS 40.0%
TTCATGCACAGTGATTCACA+TGG - chr4.3:81959940-81959959 None:intergenic 40.0%
TTCTTTACTCGGCGTGATAT+CGG + chr4.3:81962067-81962086 MS.gene037550:CDS 40.0%
TTGATGTCAAGAATGCTCCA+AGG - chr4.3:81961791-81961810 None:intergenic 40.0%
! AACTCTTTTGCTGGATGTGT+TGG + chr4.3:81961172-81961191 MS.gene037550:CDS 40.0%
! ATTAACCTTGACTAGTGGAC+TGG + chr4.3:81961812-81961831 MS.gene037550:CDS 40.0%
! CAGGTCAAAAATAGGTGTTG+TGG + chr4.3:81961519-81961538 MS.gene037550:CDS 40.0%
! CCAGAAACTTTGGTCCATTT+TGG - chr4.3:81960134-81960153 None:intergenic 40.0%
! GTTTCTGTGTTACTCAATCC+TGG + chr4.3:81961229-81961248 MS.gene037550:CDS 40.0%
! TTGCAGCTGTTGATGGAATT+GGG + chr4.3:81960198-81960217 MS.gene037550:CDS 40.0%
! TTGGGATCTAACTCTTTTGC+TGG + chr4.3:81961163-81961182 MS.gene037550:CDS 40.0%
!! GATAAGCATTGGTGAAGTTG+AGG + chr4.3:81961669-81961688 MS.gene037550:CDS 40.0%
!! TTAACCTTGACTAGTGGACT+GGG + chr4.3:81961813-81961832 MS.gene037550:CDS 40.0%
!!! ACCTATTTTTGACCTGTTGG+GGG - chr4.3:81961515-81961534 None:intergenic 40.0%
!!! CACCTATTTTTGACCTGTTG+GGG - chr4.3:81961516-81961535 None:intergenic 40.0%
!!! GAATTCTTTTGGGGCAACAT+CGG + chr4.3:81962154-81962173 MS.gene037550:CDS 40.0%
!!! GTGATATCGGCTTCCATTTT+CGG + chr4.3:81962080-81962099 MS.gene037550:CDS 40.0%
AAAGACGGTATAGAAGCTGC+CGG - chr4.3:81962329-81962348 None:intergenic 45.0%
AACAAATGTTGCTGCATCCC+AGG + chr4.3:81959911-81959930 MS.gene037550:CDS 45.0%
AATATTGCGGGTGCCCAAAA+TGG + chr4.3:81960117-81960136 MS.gene037550:CDS 45.0%
ACCTACACTCAGACGATGTT+TGG + chr4.3:81959872-81959891 MS.gene037550:CDS 45.0%
CAGGAACACCAAAGAGAAAG+AGG + chr4.3:81961329-81961348 MS.gene037550:CDS 45.0%
CAGTGATTCACATGGATTCC+TGG - chr4.3:81959932-81959951 None:intergenic 45.0%
CCAAACGGAGTTGAAGATGT+CGG - chr4.3:81962218-81962237 None:intergenic 45.0%
CCGTACGAATCCAAATTTCG+AGG - chr4.3:81960084-81960103 None:intergenic 45.0%
CCTTAATTGCACTGGAACCA+AGG + chr4.3:81961488-81961507 MS.gene037550:CDS 45.0%
CCTTGGTTCCAGTGCAATTA+AGG - chr4.3:81961491-81961510 None:intergenic 45.0%
CGGCTTCAAATACAGCTTCA+GGG + chr4.3:81962174-81962193 MS.gene037550:CDS 45.0%
CTTCGGTTATGATGCTGTTG+TGG + chr4.3:81960472-81960491 MS.gene037550:intron 45.0%
GACTTACTTGCAGCTGTTGA+TGG + chr4.3:81960191-81960210 MS.gene037550:CDS 45.0%
GAGAGGCTCATTAGAGGTTA+AGG - chr4.3:81960918-81960937 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG + chr4.3:81960243-81960262 MS.gene037550:CDS 45.0%
GAGGATCCAAAATTGACGGA+AGG - chr4.3:81962047-81962066 None:intergenic 45.0%
GATCCAAAATTGACGGAAGG+AGG - chr4.3:81962044-81962063 None:intergenic 45.0%
GCCTTTGATGTTCCAAACGA+CGG - chr4.3:81962374-81962393 None:intergenic 45.0%
GGTGTACCAGAAGATGATGA+TGG - chr4.3:81962293-81962312 None:intergenic 45.0%
GTGGGATTTGTGAAGAGAGA+AGG + chr4.3:81961538-81961557 MS.gene037550:CDS 45.0%
GTTGCCCCAAAAGAATTCGT+AGG - chr4.3:81962152-81962171 None:intergenic 45.0%
TATCACGCCGAGTAAAGAAG+AGG - chr4.3:81962066-81962085 None:intergenic 45.0%
TCCAAACATCGTCTGAGTGT+AGG - chr4.3:81959876-81959895 None:intergenic 45.0%
TCCCCCAACAGGTCAAAAAT+AGG + chr4.3:81961511-81961530 MS.gene037550:CDS 45.0%
TCGGCTTCAAATACAGCTTC+AGG + chr4.3:81962173-81962192 MS.gene037550:CDS 45.0%
TCGGCTTCTACACCATTGTT+TGG + chr4.3:81962263-81962282 MS.gene037550:CDS 45.0%
TCTGAGGAATGTTGAGTGGT+TGG - chr4.3:81961282-81961301 None:intergenic 45.0%
TGCGAAGTCCTTAATTGCAC+TGG + chr4.3:81961480-81961499 MS.gene037550:CDS 45.0%
TGGATCCTAATGGCATGGTA+AGG - chr4.3:81961132-81961151 None:intergenic 45.0%
TGTCTGTGAGGATCCAAATG+CGG - chr4.3:81961955-81961974 None:intergenic 45.0%
! ACCGTCGTTTGGAACATCAA+AGG + chr4.3:81962370-81962389 MS.gene037550:CDS 45.0%
! CATCGTCTGAGTGTAGGTTT+TGG - chr4.3:81959870-81959889 None:intergenic 45.0%
! CTTGCAGCTGTTGATGGAAT+TGG + chr4.3:81960197-81960216 MS.gene037550:CDS 45.0%
! GATGATGGTGCTCCAAACAA+TGG - chr4.3:81962278-81962297 None:intergenic 45.0%
! GCAGCTTCTATACCGTCTTT+TGG + chr4.3:81962329-81962348 MS.gene037550:CDS 45.0%
! GGAATGTTGAGTGGTTGGTT+TGG - chr4.3:81961277-81961296 None:intergenic 45.0%
! TAAGCAGGGTGTTCAATCTG+AGG - chr4.3:81961298-81961317 None:intergenic 45.0%
! TCTTCTGGTACACCGTCTTT+TGG + chr4.3:81962299-81962318 MS.gene037550:CDS 45.0%
! TGCAGCTGTTGATGGAATTG+GGG + chr4.3:81960199-81960218 MS.gene037550:CDS 45.0%
! TGGTTTGGACAACCAAACTG+TGG - chr4.3:81961262-81961281 None:intergenic 45.0%
!! GTGTAGGCTTTGAACTTCCT+TGG + chr4.3:81961771-81961790 MS.gene037550:intron 45.0%
!! TTTCGAGGCTGTTGGTTTGT+TGG - chr4.3:81960069-81960088 None:intergenic 45.0%
!!! AGTGTAGGTTTTGGACGGTT+TGG - chr4.3:81959861-81959880 None:intergenic 45.0%
!!! GTCTGAGTGTAGGTTTTGGA+CGG - chr4.3:81959866-81959885 None:intergenic 45.0%
AAATAGCGTGCCAAAGGTGG+CGG - chr4.3:81962108-81962127 None:intergenic 50.0%
AATGCGGAGGTGGATGATGA+AGG - chr4.3:81961939-81961958 None:intergenic 50.0%
ACACAACATTGGCAAGCAGG+AGG + chr4.3:81960254-81960273 MS.gene037550:CDS 50.0%
ACCAAATAGCGTGCCAAAGG+TGG - chr4.3:81962111-81962130 None:intergenic 50.0%
ACCACCAAATAGCGTGCCAA+AGG - chr4.3:81962114-81962133 None:intergenic 50.0%
ACCATTGTTTGGATCCCCAG+CGG + chr4.3:81962400-81962419 MS.gene037550:CDS 50.0%
ACCTTTGGCACGCTATTTGG+TGG + chr4.3:81962110-81962129 MS.gene037550:CDS 50.0%
AGGAGAAGAAGTTGTGGCAG+AGG - chr4.3:81961979-81961998 None:intergenic 50.0%
AGGAGATGGCAAGAAAGAGG+AGG - chr4.3:81962006-81962025 None:intergenic 50.0%
CACACACAACATTGGCAAGC+AGG + chr4.3:81960251-81960270 MS.gene037550:CDS 50.0%
CCAAAGGACGGTACTCCAAA+CGG - chr4.3:81962233-81962252 None:intergenic 50.0%
CCATCTCCTTCCTCTTCTAG+AGG + chr4.3:81962017-81962036 MS.gene037550:CDS 50.0%
CCGACATCTTCAACTCCGTT+TGG + chr4.3:81962215-81962234 MS.gene037550:CDS 50.0%
CCTGGTATAGCACCACAGTT+TGG + chr4.3:81961247-81961266 MS.gene037550:CDS 50.0%
GATGACGATGCTCCAAAGGA+CGG - chr4.3:81962245-81962264 None:intergenic 50.0%
GGAAGGAGATGGCAAGAAAG+AGG - chr4.3:81962009-81962028 None:intergenic 50.0%
GGCAGAAGGAGAAGAAGTTG+TGG - chr4.3:81961985-81962004 None:intergenic 50.0%
TCATCATCCACCTCCGCATT+TGG + chr4.3:81961939-81961958 MS.gene037550:CDS 50.0%
! ATTTTCGGTTCCGCCACCTT+TGG + chr4.3:81962095-81962114 MS.gene037550:CDS 50.0%
! CCTCTAGAAGAGGAAGGAGA+TGG - chr4.3:81962020-81962039 None:intergenic 50.0%
! GGACCTCCTTCCGTCAATTT+TGG + chr4.3:81962038-81962057 MS.gene037550:CDS 50.0%
! GGAGCACCATCATCATCTTC+TGG + chr4.3:81962284-81962303 MS.gene037550:CDS 50.0%
!! AAAGGTGGCGGAACCGAAAA+TGG - chr4.3:81962096-81962115 None:intergenic 50.0%
!! CCAAACTGTGGTGCTATACC+AGG - chr4.3:81961250-81961269 None:intergenic 50.0%
!! GTCCTTTGGAGCATCGTCAT+CGG + chr4.3:81962244-81962263 MS.gene037550:CDS 50.0%
!!! ACCGTCTTTTGGAGCTCCAA+CGG + chr4.3:81962340-81962359 MS.gene037550:CDS 50.0%
!!! GGTTTTGGACGGTTTGGTAG+AGG - chr4.3:81959855-81959874 None:intergenic 50.0%
!!! TACACCGTCTTTTGGAGCAC+CGG + chr4.3:81962307-81962326 MS.gene037550:CDS 50.0%
AACGACGGTGTAGAAGCCGT+TGG - chr4.3:81962359-81962378 None:intergenic 55.0%
ACAGCTTCAGGGTCGTCGTT+TGG + chr4.3:81962185-81962204 MS.gene037550:CDS 55.0%
ACGGCTTCTACACCGTCGTT+TGG + chr4.3:81962359-81962378 MS.gene037550:CDS 55.0%
AGCCGATGACGATGCTCCAA+AGG - chr4.3:81962249-81962268 None:intergenic 55.0%
CGGAGGTGGATGATGAAGGA+GGG - chr4.3:81961935-81961954 None:intergenic 55.0%
CTGTGAGGATCCAAATGCGG+AGG - chr4.3:81961952-81961971 None:intergenic 55.0%
GCCACCTTTGGCACGCTATT+TGG + chr4.3:81962107-81962126 MS.gene037550:CDS 55.0%
GCCGTTGGAGCTCCAAAAGA+CGG - chr4.3:81962344-81962363 None:intergenic 55.0%
GGCAAGAAAGAGGAGGCAGA+AGG - chr4.3:81961999-81962018 None:intergenic 55.0%
GTCTCCCAGTCCACTAGTCA+AGG - chr4.3:81961820-81961839 None:intergenic 55.0%
TGAGGATCCAAATGCGGAGG+TGG - chr4.3:81961949-81961968 None:intergenic 55.0%
TGAGGCATGCTTCACCCTTC+AGG + chr4.3:81960528-81960547 MS.gene037550:intron 55.0%
TGTGGCAGAGGTTGTCTGTG+AGG - chr4.3:81961967-81961986 None:intergenic 55.0%
TTTGACCTGTTGGGGGACCT+TGG - chr4.3:81961508-81961527 None:intergenic 55.0%
! GGAAGGAGGTCCTCTAGAAG+AGG - chr4.3:81962030-81962049 None:intergenic 55.0%
! GGAGGTCCTCTAGAAGAGGA+AGG - chr4.3:81962026-81962045 None:intergenic 55.0%
! TTGCGCCGTCGTTTGGAGTT+TGG + chr4.3:81962426-81962445 MS.gene037550:CDS 55.0%
!! CCGTTTGGAGTACCGTCCTT+TGG + chr4.3:81962230-81962249 MS.gene037550:CDS 55.0%
GCCGCTGGGGATCCAAACAA+TGG - chr4.3:81962404-81962423 None:intergenic 60.0%
GCGGAGGTGGATGATGAAGG+AGG - chr4.3:81961936-81961955 None:intergenic 60.0%
GCTGCCGGTGCTCCAAAAGA+CGG - chr4.3:81962314-81962333 None:intergenic 60.0%
TGGAACCAAGGTCCCCCAAC+AGG + chr4.3:81961500-81961519 MS.gene037550:CDS 60.0%
AACGACGGCGCAAAAGCCGC+TGG - chr4.3:81962419-81962438 None:intergenic 65.0%
ACGACGGCGCAAAAGCCGCT+GGG - chr4.3:81962418-81962437 None:intergenic 65.0%
! GCGGCTTTTGCGCCGTCGTT+TGG + chr4.3:81962419-81962438 MS.gene037550:CDS 65.0%
CGACGGCGCAAAAGCCGCTG+GGG - chr4.3:81962417-81962436 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 81959838 81962454 81959838 ID=MS.gene037550
chr4.3 mRNA 81959838 81962454 81959838 ID=MS.gene037550.t1;Parent=MS.gene037550
chr4.3 exon 81959838 81960275 81959838 ID=MS.gene037550.t1.exon1;Parent=MS.gene037550.t1
chr4.3 CDS 81959838 81960275 81959838 ID=cds.MS.gene037550.t1;Parent=MS.gene037550.t1
chr4.3 exon 81960893 81961690 81960893 ID=MS.gene037550.t1.exon2;Parent=MS.gene037550.t1
chr4.3 CDS 81960893 81961690 81960893 ID=cds.MS.gene037550.t1;Parent=MS.gene037550.t1
chr4.3 exon 81961777 81962454 81961777 ID=MS.gene037550.t1.exon3;Parent=MS.gene037550.t1
chr4.3 CDS 81961777 81962454 81961777 ID=cds.MS.gene037550.t1;Parent=MS.gene037550.t1
Gene Sequence

>MS.gene037550

ATGATCAACAAAAACCTCTACCAAACCGTCCAAAACCTACACTCAGACGATGTTTGGAACACAACTTTTGTCAAACAAATGTTGCTGCATCCCAGGAATCCATGTGAATCACTGTGCATGAAGATGTTCTTGAACGTTGATGACACTGAACCATCAAACAAGTTTTTTGTGTGTGATTCTTGTAACAAGTTCACTACTTTTCAAAACCTTCATTGTACTTGTGGAAAACCAACAAACCAACAGCCTCGAAATTTGGATTCGTACGGTCAAGAGAATAGTAATATTGCGGGTGCCCAAAATGGACCAAAGTTTCTGGTGTCTGATGATTTGAAGATTGTTCCATGTTCGTCAGTGACTTACTTGCAGCTGTTGATGGAATTGGGGTATTCAGATGCGACTCAATTAGAGGAAGTCACACACAACATTGGCAAGCAGGAGGTAATTAATTCTGTTGTGTGGTTTTTTGTTTGATATGTATCTATCTCACAAGTTGTATCATTACATTGTTTTTTTTATTACTTGCATAGCAGCTTAGACCAATCTTAATCACGAATCTTCACCTCATGGTTTTAATTTGTGGTTACTTTTATTGAGAACCTTTATTATGGTAAAATTTAGAAAAATGCGCTCACAACTTCGGTTATGATGCTGTTGTGGAAACCTCTAAAATCATATATTTGTGACTGCAATTGAGGCATGCTTCACCCTTCAGGAAATGTAGTAGTACTAGGGTTTGATGGTGTATCAGTTAGTGTCGGGAACCCGAAAATTCAATAGATCTTAAATTTCAAAGTTGCAACACTAGTAGCTTGTACCAAGTTTAAAGGTTCGGAAAGGAAAAATATTAATTAATATTCACAATCTCAGGATTTAATACTTGACAAAAAAAAGAGAATATAATAAAAAAAGAGTAGAATAGTGCAGTTAGGGAAGTCAATTAGTTAGATCAGGATATTGATCACTTTATTGAAAAGTTGATGAAGTTAATTTTTGTTTAATAACTTCTTTTTTTTTTGTTTGAAAAAACTATTGACCACTTCCTTTTTGATGTACAGATATTGAAACTACTTAAGTATACCTTAACCTCTAATGAGCCTCTCACAAATACAATTTTGGCAAGCAGCTCCAAAAATAAAGACAACTTACCTAAGCAATCTGCATCAACAGTTAGAGTAATGCCTTGCACCAATAACAGAAGTAAAATTAATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGGTGATATATGCAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTAGTTTCCTTACCATGCCATTAGGATCCATTGTAAAGCTTTTGGGATCTAACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGGACCTTAACCCTGTTTCTGTGTTACTCAATCCTGGTATAGCACCACAGTTTGGTTGTCCAAACCAACCACTCAACATTCCTCAGATTGAACACCCTGCTTATTATTATGGTACAGGAACACCAAAGAGAAAGAGGGATCAACATTCCTCAGATTTCGCGGAGTATAATGGTAATCGTATAATGATGGATTGTAGTGGTAATACTGTGATAGAAGAAAAGATAGAAGGAGGAGTGATTTCTAAGTCACGCAAATCAATTTATTTTGCGAAGTCCTTAATTGCACTGGAACCAAGGTCCCCCAACAGGTCAAAAATAGGTGTTGTGGGATTTGTGAAGAGAGAAGGGTTATATTGTGTAGGAGATGATCTAAAAGCAAAACCACTTTCTGTTCATTCGCATCTTTCATTTTTGAAAGAGTTAAGCCTTCCTCTTGATGATCTTGAAATGAAGCTGATAAGCATTGGTGAAGTTGAGGTAAAGATGCTATTATTTTATCACTAAGTTTATAAAAATTAATTGTGTTATTTTAATCTCTTTGATTTCCTTTACTTGTGGTGTAGGCTTTGAACTTCCTTGGAGCATTCTTGACATCAAAATTAACCTTGACTAGTGGACTGGGAGACCTTTCGAAACTGCCTAAAATCTCAAACCCGTTTTGCAATCGAACATTCAAAACCCTTCCTCAATTTCAAAACCAAAAGGAAAAAACTTCACACCCTCCTTCATCATCCACCTCCGCATTTGGATCCTCACAGACAACCTCTGCCACAACTTCTTCTCCTTCTGCCTCCTCTTTCTTGCCATCTCCTTCCTCTTCTAGAGGACCTCCTTCCGTCAATTTTGGATCCTCTTCTTTACTCGGCGTGATATCGGCTTCCATTTTCGGTTCCGCCACCTTTGGCACGCTATTTGGTGGTAGAGCTCCTACTACTCCTACGAATTCTTTTGGGGCAACATCGGCTTCAAATACAGCTTCAGGGTCGTCGTTTGGAGCATCACCGACATCTTCAACTCCGTTTGGAGTACCGTCCTTTGGAGCATCGTCATCGGCTTCTACACCATTGTTTGGAGCACCATCATCATCTTCTGGTACACCGTCTTTTGGAGCACCGGCAGCTTCTATACCGTCTTTTGGAGCTCCAACGGCTTCTACACCGTCGTTTGGAACATCAAAGGCTTCTACACCATTGTTTGGATCCCCAGCGGCTTTTGCGCCGTCGTTTGGAGTTTGGTTTTGA
Protein sequence

>MS.gene037550.t1

MINKNLYQTVQNLHSDDVWNTTFVKQMLLHPRNPCESLCMKMFLNVDDTEPSNKFFVCDSCNKFTTFQNLHCTCGKPTNQQPRNLDSYGQENSNIAGAQNGPKFLVSDDLKIVPCSSVTYLQLLMELGYSDATQLEEVTHNIGKQEILKLLKYTLTSNEPLTNTILASSSKNKDNLPKQSASTVRVMPCTNNRSKINVKVVQSKSQKKVIYAEANGDFVDFIFSFLTMPLGSIVKLLGSNSFAGCVGNLYKSVEDLNPVSVLLNPGIAPQFGCPNQPLNIPQIEHPAYYYGTGTPKRKRDQHSSDFAEYNGNRIMMDCSGNTVIEEKIEGGVISKSRKSIYFAKSLIALEPRSPNRSKIGVVGFVKREGLYCVGDDLKAKPLSVHSHLSFLKELSLPLDDLEMKLISIGEVEALNFLGAFLTSKLTLTSGLGDLSKLPKISNPFCNRTFKTLPQFQNQKEKTSHPPSSSTSAFGSSQTTSATTSSPSASSFLPSPSSSRGPPSVNFGSSSLLGVISASIFGSATFGTLFGGRAPTTPTNSFGATSASNTASGSSFGASPTSSTPFGVPSFGASSSASTPLFGAPSSSSGTPSFGAPAASIPSFGAPTASTPSFGTSKASTPLFGSPAAFAPSFGVWF