AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99475

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99475.t1 MTR_4g019340 81.250 400 50 2 1 400 128 502 0.0 643
MS.gene99475.t1 MTR_4g017830 71.364 440 114 4 1 437 97 527 0.0 615
MS.gene99475.t1 MTR_4g017440 68.750 416 115 5 1 415 105 506 0.0 546
MS.gene99475.t1 MTR_4g017410 64.303 423 123 5 1 421 98 494 0.0 521
MS.gene99475.t1 MTR_4g017410 27.203 261 125 8 179 405 12 241 2.68e-14 75.1
MS.gene99475.t1 MTR_4g017550 66.184 414 120 8 1 411 77 473 1.09e-176 503
MS.gene99475.t1 MTR_4g017470 60.047 423 124 3 1 421 101 480 1.18e-162 468
MS.gene99475.t1 MTR_4g017470 27.165 254 108 8 172 407 10 204 4.63e-11 65.1
MS.gene99475.t1 MTR_4g017610 57.002 407 144 7 1 407 100 475 3.25e-148 430
MS.gene99475.t1 MTR_4g019310 42.553 423 210 13 1 415 95 492 3.02e-91 285
MS.gene99475.t1 MTR_4g019310 28.629 248 147 6 173 404 5 238 2.82e-18 87.4
MS.gene99475.t1 MTR_7g066340 32.967 455 218 13 1 439 91 474 3.70e-60 203
MS.gene99475.t1 MTR_7g066340 28.333 240 141 7 176 404 12 231 3.70e-19 90.1
MS.gene99475.t1 MTR_7g066570 34.899 447 218 16 1 422 93 491 3.83e-58 200
MS.gene99475.t1 MTR_7g066570 26.923 234 137 6 179 404 17 224 3.69e-12 68.6
MS.gene99475.t1 MTR_7g066260 32.009 453 236 11 1 438 94 489 2.71e-55 191
MS.gene99475.t1 MTR_7g066260 29.046 241 138 9 176 404 15 234 8.78e-14 73.6
MS.gene99475.t1 MTR_7g066400 34.286 420 202 11 1 407 75 433 5.27e-55 189
MS.gene99475.t1 MTR_7g066410 33.042 457 227 15 1 411 89 512 5.99e-55 191
MS.gene99475.t1 MTR_7g058710 31.797 434 250 11 1 417 79 483 7.15e-55 190
MS.gene99475.t1 MTR_7g058710 26.724 232 145 5 176 404 12 221 2.61e-15 78.2
MS.gene99475.t1 MTR_7g066350 32.857 420 207 10 1 407 88 445 2.74e-49 174
MS.gene99475.t1 MTR_7g066350 28.458 253 150 6 176 420 9 238 6.36e-18 86.3
MS.gene99475.t1 MTR_7g066430 34.325 437 233 16 1 414 83 488 2.27e-48 172
MS.gene99475.t1 MTR_7g062280 33.108 444 229 16 1 417 89 491 1.79e-46 167
MS.gene99475.t1 MTR_7g062280 31.174 247 133 10 177 404 11 239 1.75e-14 75.9
MS.gene99475.t1 MTR_7g012680 33.023 430 190 15 1 410 93 444 1.19e-44 162
MS.gene99475.t1 MTR_7g012680 25.703 249 139 8 164 405 2 211 2.17e-12 68.9
MS.gene99475.t1 MTR_7g066360 32.965 452 223 15 1 439 93 477 1.33e-44 162
MS.gene99475.t1 MTR_7g063660 32.483 431 236 15 1 417 88 477 3.73e-43 158
MS.gene99475.t1 MTR_7g063660 29.412 238 135 8 176 404 9 222 4.03e-16 80.9
MS.gene99475.t1 MTR_7g066580 30.788 419 221 12 1 404 74 438 1.62e-41 153
MS.gene99475.t1 MTR_1g041315 30.425 424 201 14 1 395 93 451 4.61e-41 152
MS.gene99475.t1 MTR_7g066280 28.381 451 244 13 1 422 1 401 7.13e-41 150
MS.gene99475.t1 MTR_7g063880 30.166 421 236 14 1 411 90 462 1.52e-40 151
MS.gene99475.t1 MTR_7g063880 28.750 240 135 8 176 404 11 225 4.06e-13 71.2
MS.gene99475.t1 MTR_7g063900 33.019 424 241 14 1 411 89 482 1.72e-40 151
MS.gene99475.t1 MTR_7g063900 29.150 247 138 8 170 404 4 225 2.17e-16 81.6
MS.gene99475.t1 MTR_7g062300 31.905 420 238 15 1 410 91 472 1.93e-40 150
MS.gene99475.t1 MTR_7g062300 28.216 241 138 7 176 404 12 229 2.40e-13 72.0
MS.gene99475.t1 MTR_7g063840 30.841 428 242 15 1 417 89 473 1.40e-39 148
MS.gene99475.t1 MTR_7g066310 29.493 434 169 13 1 422 86 394 7.81e-39 145
MS.gene99475.t1 MTR_7g063890 32.477 428 237 16 1 411 106 498 2.35e-37 142
MS.gene99475.t1 MTR_7g063820 31.221 426 255 12 1 417 88 484 3.23e-37 142
MS.gene99475.t1 MTR_7g063820 34.259 108 65 2 170 271 4 111 1.04e-11 67.0
MS.gene99475.t1 MTR_7g066290 36.525 282 150 9 1 267 94 361 2.46e-35 136
MS.gene99475.t1 MTR_4g019240 89.041 73 8 0 369 441 112 184 1.84e-34 127
MS.gene99475.t1 MTR_7g063670 30.698 430 229 16 2 417 83 457 1.43e-33 131
MS.gene99475.t1 MTR_7g058700 32.759 290 129 10 1 259 584 838 1.10e-32 131
MS.gene99475.t1 MTR_7g063920 28.741 421 222 12 1 417 90 436 3.35e-32 127
MS.gene99475.t1 MTR_3g065200 28.713 404 213 14 1 397 72 407 2.69e-31 125
MS.gene99475.t1 MTR_3g007740 29.508 427 223 18 2 411 77 442 1.28e-28 117
MS.gene99475.t1 MTR_7g063830 30.847 295 191 5 1 292 89 373 1.60e-28 117
MS.gene99475.t1 MTR_7g063830 28.216 241 130 8 179 404 13 225 1.23e-13 72.8
MS.gene99475.t1 MTR_7g066680 32.075 318 158 7 128 407 37 334 7.66e-28 113
MS.gene99475.t1 MTR_7g063720 31.933 238 124 3 173 407 20 222 5.44e-26 105
MS.gene99475.t1 MTR_8g445860 30.709 254 118 8 176 405 13 232 1.22e-22 96.7
MS.gene99475.t1 MTR_4g019350 50.505 99 36 2 234 332 1 86 4.16e-22 90.5
MS.gene99475.t1 MTR_7g066480 33.455 275 142 11 1 258 95 345 5.46e-21 95.1
MS.gene99475.t1 MTR_7g066480 38.835 103 52 1 320 411 319 421 4.75e-12 67.8
MS.gene99475.t1 MTR_7g100420 31.560 282 169 7 128 407 134 393 9.19e-21 94.4
MS.gene99475.t1 MTR_2g029400 28.313 332 168 9 86 399 124 403 4.88e-20 92.0
MS.gene99475.t1 MTR_1g014060 35.714 210 96 7 206 411 210 384 5.71e-20 91.7
MS.gene99475.t1 MTR_8g023240 25.000 352 213 11 75 409 170 487 2.98e-18 87.4
MS.gene99475.t1 MTR_1g115440 30.544 239 141 7 176 405 11 233 8.23e-18 82.8
MS.gene99475.t1 MTR_7g066490 31.321 265 147 10 1 258 95 331 1.36e-17 84.7
MS.gene99475.t1 MTR_7g066490 49.296 71 36 0 341 411 337 407 1.63e-11 66.2
MS.gene99475.t1 MTR_0150s0010 30.612 245 133 8 176 405 10 232 1.54e-17 82.0
MS.gene99475.t1 MTR_1g115450 43.038 79 43 1 176 252 7 85 7.01e-14 68.9
MS.gene99475.t1 MTR_1g115455 40.506 79 45 1 176 252 9 87 1.12e-12 67.8
MS.gene99475.t1 MTR_0150s0030 34.694 98 55 2 176 270 8 99 1.49e-12 67.0
MS.gene99475.t1 MTR_8g445910 35.714 98 54 2 176 270 8 99 3.44e-12 67.0
MS.gene99475.t1 MTR_8g044880 40.476 84 46 2 176 256 8 90 8.92e-12 65.9
MS.gene99475.t1 MTR_8g445920 43.548 62 35 0 176 237 10 71 1.03e-11 62.0
MS.gene99475.t1 MTR_8g044890 43.548 62 35 0 176 237 10 71 1.03e-11 62.0
MS.gene99475.t1 MTR_4g019330 42.222 90 49 2 328 416 97 184 8.06e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99475.t1 AT5G01140 32.444 450 243 16 1 439 72 471 1.85e-43 159
MS.gene99475.t1 AT5G01120 30.095 422 256 13 1 407 88 485 4.28e-41 153
MS.gene99475.t1 AT5G43240 29.748 437 242 15 1 407 88 489 2.12e-37 143
MS.gene99475.t1 AT5G43240 29.748 437 242 15 1 407 88 489 2.12e-37 143
MS.gene99475.t1 AT5G43240 29.748 437 242 15 1 407 88 489 2.12e-37 143
MS.gene99475.t1 AT5G01130 29.268 410 230 12 1 407 88 440 3.92e-37 141
MS.gene99475.t1 AT3G09140 29.358 436 245 14 1 407 88 489 8.45e-35 135
MS.gene99475.t1 AT3G09140 28.295 258 164 7 172 418 5 252 5.22e-17 83.6
MS.gene99475.t1 AT5G01150 30.901 466 239 16 1 438 88 498 3.01e-34 134
MS.gene99475.t1 AT5G43240 29.891 368 206 11 58 407 12 345 4.35e-30 120
MS.gene99475.t1 AT3G09140 27.586 464 245 15 1 407 88 517 4.60e-30 122
MS.gene99475.t1 AT3G09140 28.436 211 136 5 172 372 5 210 3.53e-15 78.2
MS.gene99475.t1 AT3G09110 32.794 247 140 7 1 237 88 318 1.28e-26 110
MS.gene99475.t1 AT5G37320 26.573 429 261 12 1 412 89 480 1.26e-25 109
MS.gene99475.t1 AT3G09120 32.353 238 145 7 1 230 84 313 8.35e-25 104
MS.gene99475.t1 AT3G09120 28.870 239 146 8 176 405 6 229 4.19e-17 82.4

Find 95 sgRNAs with CRISPR-Local

Find 292 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAGACAGCCTAAGAATT+TGG 0.219049 4.2:+83167937 MS.gene99475:CDS
TGGTAGGTTATCTTTCTTCT+TGG 0.272752 4.2:-83170606 None:intergenic
CTCACCACTACATATTATTA+TGG 0.301079 4.2:+83170968 MS.gene99475:CDS
ATGCACCCGTGACATTAAAA+TGG 0.306519 4.2:+83170661 MS.gene99475:CDS
ATAACTCAGTGTACAAGTTT+AGG 0.324914 4.2:-83171831 None:intergenic
CGTAAATCCCTTTCTTGCTT+TGG 0.330917 4.2:-83171001 None:intergenic
ATGCAGATGTTGGTGGAGTT+AGG 0.331679 4.2:+83168088 MS.gene99475:CDS
ATCGATCTGTTCGGAGATCT+TGG 0.334754 4.2:-83171130 None:intergenic
GAAGATTGTGCCTAGTTCTT+TGG 0.339006 4.2:+83168057 MS.gene99475:CDS
AGGGTCCATTGTAAAGGTTT+TGG 0.343635 4.2:+83170784 MS.gene99475:CDS
AATGATTCACATGGATTCTT+CGG 0.347163 4.2:-83167794 None:intergenic
AGCTTCCTTACAATACCATT+AGG 0.351633 4.2:+83170764 MS.gene99475:CDS
ATTTGTCAATGGCTCATTAG+AGG 0.354868 4.2:-83170564 None:intergenic
CTCTGACTGCTGATGCAAAC+TGG 0.370802 4.2:-83170631 None:intergenic
TTCAAAATTGTATTTGTCAA+TGG 0.373137 4.2:-83170575 None:intergenic
GAATTTGGATTCTGAGGGTC+AGG 0.378169 4.2:+83167952 MS.gene99475:CDS
CACACACAACATTGGAAAAC+AGG 0.378988 4.2:+83168141 MS.gene99475:CDS
GCTTCCTTACAATACCATTA+GGG 0.381396 4.2:+83170765 MS.gene99475:CDS
CCCTCAGAATCCAAATTCTT+AGG 0.386443 4.2:-83167947 None:intergenic
CAAAGTGTGTTGAAACTAGT+AGG 0.386601 4.2:+83171787 MS.gene99475:CDS
GGAATGTTGAGGGGCTGGTT+TGG 0.395287 4.2:-83170932 None:intergenic
TTCTGATTTGAGTCAATTAG+AGG 0.405222 4.2:+83168114 MS.gene99475:CDS
TGTATTGCTGATGCTTTGAA+TGG 0.406254 4.2:+83167986 MS.gene99475:CDS
AGGAGTGATTTCTAAGACAA+TGG 0.423140 4.2:+83171069 MS.gene99475:CDS
AATCACTTTCTGCTAACTCT+TGG 0.425000 4.2:+83171218 MS.gene99475:CDS
TACTTCTACTGTATTATAAC+GGG 0.436374 4.2:-83171035 None:intergenic
CAGAATCTTGATTGTACTTG+TGG 0.438965 4.2:+83167905 MS.gene99475:CDS
ATTCTTAGGCTGTCTATTAG+TGG 0.442822 4.2:-83167933 None:intergenic
ACTGCTGATGCAAACTGGTT+TGG 0.450650 4.2:-83170626 None:intergenic
TTCTTAGGCTGTCTATTAGT+GGG 0.450651 4.2:-83167932 None:intergenic
CAGGAACACCAAAGCAAGAA+AGG 0.451058 4.2:+83170993 MS.gene99475:CDS
TGCAGATGTTGGTGGAGTTA+GGG 0.452689 4.2:+83168089 MS.gene99475:CDS
GTATTGCTGATGCTTTGAAT+GGG 0.453410 4.2:+83167987 MS.gene99475:CDS
ACCATTAGGGTCCATTGTAA+AGG 0.460196 4.2:+83170778 MS.gene99475:CDS
TCTGAGGGTCAGGGGAATAG+TGG 0.460813 4.2:+83167962 MS.gene99475:CDS
GTACTTCTACTGTATTATAA+CGG 0.466663 4.2:-83171036 None:intergenic
GATTGTGCCTAGTTCTTTGG+TGG 0.468949 4.2:+83168060 MS.gene99475:CDS
CCTGGTGTCGCACCACAGTT+TGG 0.469731 4.2:+83170905 MS.gene99475:CDS
CGCTCCGTGTTACTCAATCC+TGG 0.470619 4.2:+83170887 MS.gene99475:CDS
TATGAGGAATGTTGAGGGGC+TGG 0.477325 4.2:-83170937 None:intergenic
ACCTTTACAATGGACCCTAA+TGG 0.477568 4.2:-83170779 None:intergenic
CTTGGACTCTTTGTGGTGTA+CGG 0.490020 4.2:+83170859 MS.gene99475:CDS
TAGAGAACTTGGACTCTTTG+TGG 0.493299 4.2:+83170852 MS.gene99475:CDS
GCCTAAGAATTTGGATTCTG+AGG 0.494703 4.2:+83167946 MS.gene99475:CDS
AGACCAGCATTATATGTTGT+AGG 0.499652 4.2:+83171178 MS.gene99475:CDS
CCAAACTGTGGTGCGACACC+AGG 0.503303 4.2:-83170905 None:intergenic
TCTTGGATCCATTGCAGTCA+AGG 0.503976 4.2:-83171113 None:intergenic
GTACAAGAGTGTAGAGAACT+TGG 0.512946 4.2:+83170841 MS.gene99475:CDS
GGTGGCTTCCATGCAGATGT+TGG 0.516496 4.2:+83168078 MS.gene99475:CDS
GAAATCACTCCTCCCACTAC+AGG 0.518920 4.2:-83171058 None:intergenic
ACTACATATTATTATGGTAC+AGG 0.521118 4.2:+83170974 MS.gene99475:CDS
TGAATATTGATGACACTGAA+TGG 0.522820 4.2:+83167834 MS.gene99475:CDS
TGCAAAAGCCTTGACTGCAA+TGG 0.525800 4.2:+83171105 MS.gene99475:CDS
TGGACCCTAATGGTATTGTA+AGG 0.526033 4.2:-83170769 None:intergenic
ACATCTTTAATCGATCTGTT+CGG 0.526928 4.2:-83171139 None:intergenic
GGAGTGATTTCTAAGACAAT+GGG 0.529024 4.2:+83171070 MS.gene99475:CDS
ATGGAAGCCACCAAAGAACT+AGG 0.533783 4.2:-83168067 None:intergenic
CCTTCACTTCAAGATCATCA+AGG 0.544271 4.2:-83171270 None:intergenic
GGCTTCCATGCAGATGTTGG+TGG 0.546842 4.2:+83168081 MS.gene99475:CDS
TCTCCTACAACATATAATGC+TGG 0.557766 4.2:-83171181 None:intergenic
TTGATATCAAAGAAGCTGCT+AGG 0.558042 4.2:-83171698 None:intergenic
AGGAACACCAAAGCAAGAAA+GGG 0.568229 4.2:+83170994 MS.gene99475:CDS
TTGTATATGAGGAATGTTGA+GGG 0.573653 4.2:-83170942 None:intergenic
CAGATCGATTAAAGATGTGG+TGG 0.575124 4.2:+83171144 MS.gene99475:CDS
TACAGTAGAAGTACCTGTAG+TGG 0.576097 4.2:+83171045 MS.gene99475:CDS
CTTCACTTCAAGATCATCAA+GGG 0.577068 4.2:-83171269 None:intergenic
TCAAAATCTACACTGACTAG+TGG 0.579892 4.2:+83171717 MS.gene99475:CDS
ATTTGGATTCTGAGGGTCAG+GGG 0.580968 4.2:+83167954 MS.gene99475:CDS
GAGGAAGTCACACACAACAT+TGG 0.582955 4.2:+83168133 MS.gene99475:CDS
ACCATCCAAAACCTTTACAA+TGG 0.583435 4.2:-83170789 None:intergenic
CCTAAGAATTTGGATTCTGA+GGG 0.584987 4.2:+83167947 MS.gene99475:CDS
AAAGTGTGTTGAAACTAGTA+GGG 0.586448 4.2:+83171788 MS.gene99475:CDS
CTGATGCAAACTGGTTTGGT+AGG 0.598901 4.2:-83170622 None:intergenic
ACAGTAGAAGTACCTGTAGT+GGG 0.600137 4.2:+83171046 MS.gene99475:CDS
GTGGTGAGTGTTTGTATATG+AGG 0.601347 4.2:-83170953 None:intergenic
TATTGCTGATGCTTTGAATG+GGG 0.608870 4.2:+83167988 MS.gene99475:CDS
GAAGTGAAGGTGATTAGCAT+TGG 0.609659 4.2:+83171283 MS.gene99475:CDS
AATTTGGATTCTGAGGGTCA+GGG 0.610725 4.2:+83167953 MS.gene99475:CDS
GAACAGATCGATTAAAGATG+TGG 0.610884 4.2:+83171141 MS.gene99475:CDS
CCTTGATGATCTTGAAGTGA+AGG 0.611182 4.2:+83171270 MS.gene99475:CDS
TAACTCCACCAACATCTGCA+TGG 0.611734 4.2:-83168086 None:intergenic
TTCTTGCATAATGATTCACA+TGG 0.617558 4.2:-83167803 None:intergenic
AGATCGATTAAAGATGTGGT+GGG 0.625379 4.2:+83171145 MS.gene99475:CDS
AGGCTAAGCTGTACTTGAGG+AGG 0.645177 4.2:-83170544 None:intergenic
TGTACCATAATAATATGTAG+TGG 0.647988 4.2:-83170972 None:intergenic
GATTAGCATTGGTGAAGCCG+AGG 0.652499 4.2:+83171294 MS.gene99475:CDS
ATATCAAAGAAGCTGCTAGG+AGG 0.664960 4.2:-83171695 None:intergenic
TAGAGGCTAAGCTGTACTTG+AGG 0.670253 4.2:-83170547 None:intergenic
TTTGTATATGAGGAATGTTG+AGG 0.676554 4.2:-83170943 None:intergenic
TTCACTTCAAGATCATCAAG+GGG 0.677198 4.2:-83171268 None:intergenic
ACACAACATTGGAAAACAGG+AGG 0.691626 4.2:+83168144 MS.gene99475:CDS
TGTATATGAGGAATGTTGAG+GGG 0.697312 4.2:-83170941 None:intergenic
GTAGAAGTACCTGTAGTGGG+AGG 0.706078 4.2:+83171049 MS.gene99475:CDS
TGGTTTGGACAACCAAACTG+TGG 0.732553 4.2:-83170917 None:intergenic
GACACCAGGATTGAGTAACA+CGG 0.757821 4.2:-83170891 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATAAAATGAAAATAGAAAA+TGG + chr4.2:83170257-83170276 MS.gene99475:intron 10.0%
!! AAGTTCAAACAATAAATTAT+AGG - chr4.2:83169055-83169074 None:intergenic 15.0%
!! AAAGAATATAAAGGAAAGTA+AGG + chr4.2:83169218-83169237 MS.gene99475:intron 20.0%
!! AATGGTTTCACAAAATTAAA+CGG + chr4.2:83170275-83170294 MS.gene99475:intron 20.0%
!! ATTTGCCATTAAAATAAACT+TGG + chr4.2:83169888-83169907 MS.gene99475:intron 20.0%
!! GAAAAAATGATGTAATAAGT+AGG + chr4.2:83169283-83169302 MS.gene99475:intron 20.0%
!! TAACAGAATGTAATAAGTAA+GGG + chr4.2:83169548-83169567 MS.gene99475:intron 20.0%
!! TTAGTTGTATTTATTAACCA+AGG + chr4.2:83169961-83169980 MS.gene99475:intron 20.0%
!! TTCAAAATTGTATTTGTCAA+TGG - chr4.2:83170578-83170597 None:intergenic 20.0%
!! TTGATATTGTAGTAAAATGA+CGG + chr4.2:83168715-83168734 MS.gene99475:intron 20.0%
!! TTTGCCATTAAAATAAACTT+GGG + chr4.2:83169889-83169908 MS.gene99475:intron 20.0%
!!! AAATACAACTAACACATTTT+AGG - chr4.2:83169953-83169972 None:intergenic 20.0%
!!! TATATAACTTGATGCTTTTA+GGG + chr4.2:83169125-83169144 MS.gene99475:intron 20.0%
!!! TTAATTAGTGTTTTTGTCAT+TGG + chr4.2:83170322-83170341 MS.gene99475:intron 20.0%
!!! TTTTTAGTAAACAATCATGT+TGG + chr4.2:83171357-83171376 MS.gene99475:intron 20.0%
! AAAATGATGTAATAAGTAGG+AGG + chr4.2:83169286-83169305 MS.gene99475:intron 25.0%
! AAATGATGTAATAAGTAGGA+GGG + chr4.2:83169287-83169306 MS.gene99475:intron 25.0%
! AATATAAAGGAAAGTAAGGA+GGG + chr4.2:83169222-83169241 MS.gene99475:intron 25.0%
! AATGGAAAGGAAAATAAGTA+GGG + chr4.2:83169489-83169508 MS.gene99475:intron 25.0%
! AATTAAACAATGAGCCATTA+AGG - chr4.2:83170308-83170327 None:intergenic 25.0%
! ACAAACAAGTTTACATTACT+TGG - chr4.2:83170482-83170501 None:intergenic 25.0%
! ACTACATATTATTATGGTAC+AGG + chr4.2:83170974-83170993 MS.gene99475:CDS 25.0%
! AGAACTATGTTGTTAATTGT+TGG + chr4.2:83168830-83168849 MS.gene99475:intron 25.0%
! ATGGTTTCACAAAATTAAAC+GGG + chr4.2:83170276-83170295 MS.gene99475:intron 25.0%
! CTATGTGAGAAAGAATATAA+AGG + chr4.2:83169209-83169228 MS.gene99475:intron 25.0%
! CTTTATATTCTTTCTCACAT+AGG - chr4.2:83169211-83169230 None:intergenic 25.0%
! GAAAGTTCATAATTCAAGAA+TGG - chr4.2:83169101-83169120 None:intergenic 25.0%
! GGAAAAAAATTATAAACTGC+AGG - chr4.2:83169628-83169647 None:intergenic 25.0%
! GTAACAGAATGTAATAAGTA+AGG + chr4.2:83169547-83169566 MS.gene99475:intron 25.0%
! GTACTTCTACTGTATTATAA+CGG - chr4.2:83171039-83171058 None:intergenic 25.0%
! TAATTGGGTAAAAGAGAATA+TGG + chr4.2:83169761-83169780 MS.gene99475:intron 25.0%
! TACATGATGTTAAAACGAAA+CGG - chr4.2:83170453-83170472 None:intergenic 25.0%
! TACTTCTACTGTATTATAAC+GGG - chr4.2:83171038-83171057 None:intergenic 25.0%
! TATTAACAAGGAAAATGTGT+CGG + chr4.2:83170002-83170021 MS.gene99475:intron 25.0%
! TCCTAAAACTTAAGATAGTT+TGG + chr4.2:83169397-83169416 MS.gene99475:intron 25.0%
! TGTACCATAATAATATGTAG+TGG - chr4.2:83170975-83170994 None:intergenic 25.0%
! TTGCCATTAAAATAAACTTG+GGG + chr4.2:83169890-83169909 MS.gene99475:intron 25.0%
!! AAATGTTTTCACAAAGTACA+CGG - chr4.2:83169917-83169936 None:intergenic 25.0%
!! CTATATAACTTGATGCTTTT+AGG + chr4.2:83169124-83169143 MS.gene99475:intron 25.0%
!! CTTAGGTTTACTTGTTAATA+AGG - chr4.2:83170197-83170216 None:intergenic 25.0%
!! GTTTCAGTCTAAGATTATTT+GGG + chr4.2:83168855-83168874 MS.gene99475:intron 25.0%
!! TCCAAACTATCTTAAGTTTT+AGG - chr4.2:83169401-83169420 None:intergenic 25.0%
!! TGTTTCAGTCTAAGATTATT+TGG + chr4.2:83168854-83168873 MS.gene99475:intron 25.0%
!!! AAACAAAAGAGTACTTAATC+AGG - chr4.2:83171619-83171638 None:intergenic 25.0%
!!! CTAAAAGCATCAAGTTATAT+AGG - chr4.2:83169126-83169145 None:intergenic 25.0%
!!! GAGTTTTGAAAAAAAACTGT+AGG + chr4.2:83168612-83168631 MS.gene99475:intron 25.0%
!!! GTCATTTTACTACAATATCA+AGG - chr4.2:83168716-83168735 None:intergenic 25.0%
!!! TAACATCCATTTTAATGTCA+CGG - chr4.2:83170670-83170689 None:intergenic 25.0%
AAAGTGTGTTGAAACTAGTA+GGG + chr4.2:83171788-83171807 MS.gene99475:CDS 30.0%
AAGTAAGGAGGGAAATAAAT+TGG + chr4.2:83169233-83169252 MS.gene99475:intron 30.0%
AATGATTCACATGGATTCTT+CGG - chr4.2:83167797-83167816 None:intergenic 30.0%
ACATCTTTAATCGATCTGTT+CGG - chr4.2:83171142-83171161 None:intergenic 30.0%
AGTTTGGATTTGACATAGTA+AGG + chr4.2:83169413-83169432 MS.gene99475:intron 30.0%
CCAATGTAATCATAAGTGTT+GGG - chr4.2:83168334-83168353 None:intergenic 30.0%
CTCACCACTACATATTATTA+TGG + chr4.2:83170968-83170987 MS.gene99475:CDS 30.0%
GAATATAAAGGAAAGTAAGG+AGG + chr4.2:83169221-83169240 MS.gene99475:intron 30.0%
GAATGGAAAGGAAAATAAGT+AGG + chr4.2:83169488-83169507 MS.gene99475:intron 30.0%
GAATTGCAATAGGAATTCAA+TGG - chr4.2:83169700-83169719 None:intergenic 30.0%
GGAAAAAGATGAACTTTATC+AGG - chr4.2:83169679-83169698 None:intergenic 30.0%
GTTGGTTGTAGTTATTAACA+AGG + chr4.2:83169990-83170009 MS.gene99475:intron 30.0%
GTTTGGATTTGACATAGTAA+GGG + chr4.2:83169414-83169433 MS.gene99475:intron 30.0%
TAATAGACAGCCTAAGAATT+TGG + chr4.2:83167937-83167956 MS.gene99475:CDS 30.0%
TATTAACCAAGGAAATGTGT+TGG + chr4.2:83169972-83169991 MS.gene99475:intron 30.0%
TCCAATGTAATCATAAGTGT+TGG - chr4.2:83168335-83168354 None:intergenic 30.0%
TGAATCATTGTTCCAAATTG+TGG + chr4.2:83168666-83168685 MS.gene99475:intron 30.0%
TTACCCAATTAATTCCCTTT+GGG - chr4.2:83169752-83169771 None:intergenic 30.0%
TTCTTGCATAATGATTCACA+TGG - chr4.2:83167806-83167825 None:intergenic 30.0%
TTGATACCAAAGTTACTCTA+AGG - chr4.2:83170375-83170394 None:intergenic 30.0%
TTGTATATGAGGAATGTTGA+GGG - chr4.2:83170945-83170964 None:intergenic 30.0%
TTGTTGATACTCTTCCTTTA+TGG + chr4.2:83171650-83171669 MS.gene99475:intron 30.0%
TTTACCCAATTAATTCCCTT+TGG - chr4.2:83169753-83169772 None:intergenic 30.0%
TTTGAACTTTGAACTTGCAT+TGG + chr4.2:83169066-83169085 MS.gene99475:intron 30.0%
TTTGTATATGAGGAATGTTG+AGG - chr4.2:83170946-83170965 None:intergenic 30.0%
! CAATGTAATCATAAGTGTTG+GGG - chr4.2:83168333-83168352 None:intergenic 30.0%
! GGGTTTTTGTTAAGGAAAAT+GGG + chr4.2:83168008-83168027 MS.gene99475:CDS 30.0%
! TACTTGCTCTTTATTTCAAG+AGG - chr4.2:83170221-83170240 None:intergenic 30.0%
! TCTTTATTTCAAGAGGTCTT+AGG - chr4.2:83170214-83170233 None:intergenic 30.0%
! TGAATATTGATGACACTGAA+TGG + chr4.2:83167834-83167853 MS.gene99475:CDS 30.0%
! TTCTGATTTGAGTCAATTAG+AGG + chr4.2:83168114-83168133 MS.gene99475:CDS 30.0%
! TTTAAAGTGAGTCTTTGTGA+TGG - chr4.2:83170162-83170181 None:intergenic 30.0%
! TTTGAATGGGGTTTTTGTTA+AGG + chr4.2:83168000-83168019 MS.gene99475:CDS 30.0%
!! AACATCCATTTTAATGTCAC+GGG - chr4.2:83170669-83170688 None:intergenic 30.0%
!! AAGCGTTATCATTTTAGTCT+AGG + chr4.2:83168889-83168908 MS.gene99475:intron 30.0%
!! TCGCAATATAAAGGTTTTAG+AGG - chr4.2:83168782-83168801 None:intergenic 30.0%
AAAACTCAGTATGCAGTTCA+AGG - chr4.2:83168599-83168618 None:intergenic 35.0%
AAATAAGTAGGGAAATCGAG+TGG + chr4.2:83169500-83169519 MS.gene99475:intron 35.0%
AACAATGAGCCATTAAGGTA+AGG - chr4.2:83170303-83170322 None:intergenic 35.0%
AACACTCCTTAGAGTAACTT+TGG + chr4.2:83170366-83170385 MS.gene99475:intron 35.0%
AACCCCAAAGGGAATTAATT+GGG + chr4.2:83169746-83169765 MS.gene99475:intron 35.0%
AATCACTTTCTGCTAACTCT+TGG + chr4.2:83171218-83171237 MS.gene99475:CDS 35.0%
ACCATCCAAAACCTTTACAA+TGG - chr4.2:83170792-83170811 None:intergenic 35.0%
ACTAACACAAGACACATTCA+GGG - chr4.2:83171442-83171461 None:intergenic 35.0%
AGACCAGCATTATATGTTGT+AGG + chr4.2:83171178-83171197 MS.gene99475:CDS 35.0%
AGAGAAAGATTTCTCCTTCA+AGG + chr4.2:83169813-83169832 MS.gene99475:intron 35.0%
AGATCGATTAAAGATGTGGT+GGG + chr4.2:83171145-83171164 MS.gene99475:CDS 35.0%
AGCTTCCTTACAATACCATT+AGG + chr4.2:83170764-83170783 MS.gene99475:CDS 35.0%
AGTTCAAGGACCAACTAATT+TGG - chr4.2:83168585-83168604 None:intergenic 35.0%
ATTCTTAGGCTGTCTATTAG+TGG - chr4.2:83167936-83167955 None:intergenic 35.0%
ATTTGTCAATGGCTCATTAG+AGG - chr4.2:83170567-83170586 None:intergenic 35.0%
CAAAGTGTGTTGAAACTAGT+AGG + chr4.2:83171787-83171806 MS.gene99475:CDS 35.0%
CAATTGCAGTCGCAATATAA+AGG - chr4.2:83168791-83168810 None:intergenic 35.0%
CAGAATCTTGATTGTACTTG+TGG + chr4.2:83167905-83167924 MS.gene99475:CDS 35.0%
CCCAACACTTATGATTACAT+TGG + chr4.2:83168331-83168350 MS.gene99475:intron 35.0%
CCTAAGAATTTGGATTCTGA+GGG + chr4.2:83167947-83167966 MS.gene99475:CDS 35.0%
CTTCACTTCAAGATCATCAA+GGG - chr4.2:83171272-83171291 None:intergenic 35.0%
GAACAGATCGATTAAAGATG+TGG + chr4.2:83171141-83171160 MS.gene99475:CDS 35.0%
GAAGGAGAAATCTTTCTCTT+TGG - chr4.2:83169812-83169831 None:intergenic 35.0%
GAGAAAGATTTCTCCTTCAA+GGG + chr4.2:83169814-83169833 MS.gene99475:intron 35.0%
GATTTGTCTCCCAAATTAGT+TGG + chr4.2:83168572-83168591 MS.gene99475:intron 35.0%
GCTTCCTTACAATACCATTA+GGG + chr4.2:83170765-83170784 MS.gene99475:CDS 35.0%
GTAATAAGTAGGAGGGAATA+GGG + chr4.2:83169294-83169313 MS.gene99475:intron 35.0%
GTTCAAGGACCAACTAATTT+GGG - chr4.2:83168584-83168603 None:intergenic 35.0%
GTTGTAACTGATATAGCTGA+GGG + chr4.2:83169525-83169544 MS.gene99475:intron 35.0%
TAATAAGTAGGAGGGAATAG+GGG + chr4.2:83169295-83169314 MS.gene99475:intron 35.0%
TAATGTGACATCCCAATTCT+CGG - chr4.2:83168247-83168266 None:intergenic 35.0%
TACAGAAGTAAGTGAGAACT+GGG + chr4.2:83169576-83169595 MS.gene99475:intron 35.0%
TACCCAATTAATTCCCTTTG+GGG - chr4.2:83169751-83169770 None:intergenic 35.0%
TACTAACACAAGACACATTC+AGG - chr4.2:83171443-83171462 None:intergenic 35.0%
TCTAGGCTTAGTTAGAATTC+TGG + chr4.2:83168906-83168925 MS.gene99475:intron 35.0%
TCTCCTACAACATATAATGC+TGG - chr4.2:83171184-83171203 None:intergenic 35.0%
TGGTAGGTTATCTTTCTTCT+TGG - chr4.2:83170609-83170628 None:intergenic 35.0%
TGGTCTTCTGTTAATCAGTT+TGG - chr4.2:83171476-83171495 None:intergenic 35.0%
TGTAATAAGTAGGAGGGAAT+AGG + chr4.2:83169293-83169312 MS.gene99475:intron 35.0%
TGTATATGAGGAATGTTGAG+GGG - chr4.2:83170944-83170963 None:intergenic 35.0%
TGTTGTAACTGATATAGCTG+AGG + chr4.2:83169524-83169543 MS.gene99475:intron 35.0%
TTACAGAAGTAAGTGAGAAC+TGG + chr4.2:83169575-83169594 MS.gene99475:intron 35.0%
TTCACTTCAAGATCATCAAG+GGG - chr4.2:83171271-83171290 None:intergenic 35.0%
TTCTTAGGCTGTCTATTAGT+GGG - chr4.2:83167935-83167954 None:intergenic 35.0%
TTGATATCAAAGAAGCTGCT+AGG - chr4.2:83171701-83171720 None:intergenic 35.0%
TTTGAGTAAACCCGAGAATT+GGG + chr4.2:83168233-83168252 MS.gene99475:intron 35.0%
TTTGATCCTATTGATCCAGA+GGG + chr4.2:83169648-83169667 MS.gene99475:intron 35.0%
! AACCTCATAAGAGTGCATTA+AGG + chr4.2:83169449-83169468 MS.gene99475:intron 35.0%
! GAAGTCAACAAAGTTTTCAC+TGG - chr4.2:83170738-83170757 None:intergenic 35.0%
! GGGGTTTTTGTTAAGGAAAA+TGG + chr4.2:83168007-83168026 MS.gene99475:CDS 35.0%
! GTATTGCTGATGCTTTGAAT+GGG + chr4.2:83167987-83168006 MS.gene99475:CDS 35.0%
! GTCGGTTGTATTTGTTAACA+AGG + chr4.2:83170020-83170039 MS.gene99475:intron 35.0%
! TAGTAATAGCCACTTTACCT+CGG - chr4.2:83171314-83171333 None:intergenic 35.0%
! TATTGCTGATGCTTTGAATG+GGG + chr4.2:83167988-83168007 MS.gene99475:CDS 35.0%
! TCAAAATCTACACTGACTAG+TGG + chr4.2:83171717-83171736 MS.gene99475:CDS 35.0%
! TGACAGTACTCACACTTTTA+AGG + chr4.2:83171592-83171611 MS.gene99475:intron 35.0%
! TGTATTGCTGATGCTTTGAA+TGG + chr4.2:83167986-83168005 MS.gene99475:CDS 35.0%
! TTCTGATTGAAGGTGTGTTT+AGG + chr4.2:83168284-83168303 MS.gene99475:intron 35.0%
! TTGGATGGTAACTCTTTTGT+TGG + chr4.2:83170803-83170822 MS.gene99475:CDS 35.0%
!! AGGAGTGATTTCTAAGACAA+TGG + chr4.2:83171069-83171088 MS.gene99475:CDS 35.0%
!! GGAGTGATTTCTAAGACAAT+GGG + chr4.2:83171070-83171089 MS.gene99475:CDS 35.0%
!! TCTGTTAGTTTGCAGCTTAT+CGG - chr4.2:83169265-83169284 None:intergenic 35.0%
!! TTTTGAGTAAACCCGAGAAT+TGG + chr4.2:83168232-83168251 MS.gene99475:intron 35.0%
!!! CTTTTAGGGTAGGATGTATT+TGG + chr4.2:83169139-83169158 MS.gene99475:intron 35.0%
!!! GGACCCCAAGTTTATTTTAA+TGG - chr4.2:83169896-83169915 None:intergenic 35.0%
!!! TAACTTGATGCTTTTAGGGT+AGG + chr4.2:83169129-83169148 MS.gene99475:intron 35.0%
!!! TCCATTGTAAAGGTTTTGGA+TGG + chr4.2:83170788-83170807 MS.gene99475:CDS 35.0%
AATGTGACATCCCAATTCTC+GGG - chr4.2:83168246-83168265 None:intergenic 40.0%
ACACAACATTGGAAAACAGG+AGG + chr4.2:83168144-83168163 MS.gene99475:CDS 40.0%
ACCATTAGGGTCCATTGTAA+AGG + chr4.2:83170778-83170797 MS.gene99475:CDS 40.0%
ACCTTTACAATGGACCCTAA+TGG - chr4.2:83170782-83170801 None:intergenic 40.0%
ACTAGCTAGCCCACAAAAAT+TGG - chr4.2:83168507-83168526 None:intergenic 40.0%
ACTGCAGGCTAATTTAAGAG+TGG - chr4.2:83169613-83169632 None:intergenic 40.0%
AGAGCCACTACTCATATACA+AGG - chr4.2:83169847-83169866 None:intergenic 40.0%
AGCCACTACTCATATACAAG+GGG - chr4.2:83169845-83169864 None:intergenic 40.0%
AGGAACACCAAAGCAAGAAA+GGG + chr4.2:83170994-83171013 MS.gene99475:CDS 40.0%
ATAGGGTTCAACAACACTTG+AGG - chr4.2:83169724-83169743 None:intergenic 40.0%
ATATCAAAGAAGCTGCTAGG+AGG - chr4.2:83171698-83171717 None:intergenic 40.0%
ATGCACCCGTGACATTAAAA+TGG + chr4.2:83170661-83170680 MS.gene99475:CDS 40.0%
CACACACAACATTGGAAAAC+AGG + chr4.2:83168141-83168160 MS.gene99475:CDS 40.0%
CACCTTAATGCACTCTTATG+AGG - chr4.2:83169454-83169473 None:intergenic 40.0%
CAGATCGATTAAAGATGTGG+TGG + chr4.2:83171144-83171163 MS.gene99475:CDS 40.0%
CATTGGAAAACAGGAGGTAA+TGG + chr4.2:83168150-83168169 MS.gene99475:intron 40.0%
CCCTCAGAATCCAAATTCTT+AGG - chr4.2:83167950-83167969 None:intergenic 40.0%
CCTTCACTTCAAGATCATCA+AGG - chr4.2:83171273-83171292 None:intergenic 40.0%
CGTAAATCCCTTTCTTGCTT+TGG - chr4.2:83171004-83171023 None:intergenic 40.0%
CTACAACCAACACATTTCCT+TGG - chr4.2:83169981-83170000 None:intergenic 40.0%
CTATGCGAGAAAGAATGGAA+AGG + chr4.2:83169476-83169495 MS.gene99475:intron 40.0%
CTCTGGATCAATAGGATCAA+AGG - chr4.2:83169649-83169668 None:intergenic 40.0%
CTGCAAAGTGCAAACCATAA+AGG - chr4.2:83171667-83171686 None:intergenic 40.0%
CTTTGATCCTATTGATCCAG+AGG + chr4.2:83169647-83169666 MS.gene99475:intron 40.0%
GAACCCCAAAGGGAATTAAT+TGG + chr4.2:83169745-83169764 MS.gene99475:intron 40.0%
GAAGTGAAGGTGATTAGCAT+TGG + chr4.2:83171283-83171302 MS.gene99475:CDS 40.0%
GAGCCACTACTCATATACAA+GGG - chr4.2:83169846-83169865 None:intergenic 40.0%
GCCTAAGAATTTGGATTCTG+AGG + chr4.2:83167946-83167965 MS.gene99475:CDS 40.0%
GTGACACCATCTCACATTTA+AGG - chr4.2:83171384-83171403 None:intergenic 40.0%
GTGGTGAGTGTTTGTATATG+AGG - chr4.2:83170956-83170975 None:intergenic 40.0%
GTTGGTCCTTAAATGTGAGA+TGG + chr4.2:83171375-83171394 MS.gene99475:intron 40.0%
TAAACGGGTCCTTACCTTAA+TGG + chr4.2:83170291-83170310 MS.gene99475:intron 40.0%
TAGAGAACTTGGACTCTTTG+TGG + chr4.2:83170852-83170871 MS.gene99475:CDS 40.0%
TAGGGTTCAACAACACTTGA+GGG - chr4.2:83169723-83169742 None:intergenic 40.0%
TCTCGAACTCATTAATCGTC+AGG - chr4.2:83168986-83169005 None:intergenic 40.0%
TGCTAGCTATGATGCATTTC+AGG - chr4.2:83170126-83170145 None:intergenic 40.0%
TGGACCCTAATGGTATTGTA+AGG - chr4.2:83170772-83170791 None:intergenic 40.0%
TGGAGAATTAGGTGTGAACT+GGG + chr4.2:83169318-83169337 MS.gene99475:intron 40.0%
TTCGTACACATCTCAATGCA+CGG - chr4.2:83170398-83170417 None:intergenic 40.0%
! AATTTGGATTCTGAGGGTCA+GGG + chr4.2:83167953-83167972 MS.gene99475:CDS 40.0%
! ACACCTTCAATCAGAAGTGT+CGG - chr4.2:83168280-83168299 None:intergenic 40.0%
! AGGGTCCATTGTAAAGGTTT+TGG + chr4.2:83170784-83170803 MS.gene99475:CDS 40.0%
! CCTTGATGATCTTGAAGTGA+AGG + chr4.2:83171270-83171289 MS.gene99475:CDS 40.0%
! CTCATAAGAGTGCATTAAGG+TGG + chr4.2:83169452-83169471 MS.gene99475:intron 40.0%
! CTTTATGGTTTGCACTTTGC+AGG + chr4.2:83171665-83171684 MS.gene99475:intron 40.0%
! GAAGATTGTGCCTAGTTCTT+TGG + chr4.2:83168057-83168076 MS.gene99475:CDS 40.0%
! GACTTTGAGAGTATACTCCA+AGG - chr4.2:83168462-83168481 None:intergenic 40.0%
! GTACAAGAGTGTAGAGAACT+TGG + chr4.2:83170841-83170860 MS.gene99475:CDS 40.0%
!! ACAGTAGAAGTACCTGTAGT+GGG + chr4.2:83171046-83171065 MS.gene99475:CDS 40.0%
!! GGAAAATGGGTCGTTGTTTT+TGG + chr4.2:83168021-83168040 MS.gene99475:CDS 40.0%
!! GTTACTCTAAGGAGTGTTTG+AGG - chr4.2:83170364-83170383 None:intergenic 40.0%
!! TACAGTAGAAGTACCTGTAG+TGG + chr4.2:83171045-83171064 MS.gene99475:CDS 40.0%
!! TGTACAGCTCTGGCTTTAAT+TGG + chr4.2:83168528-83168547 MS.gene99475:intron 40.0%
AACAGCACGTGTGTTTGTGT+AGG - chr4.2:83170423-83170442 None:intergenic 45.0%
AAGACCAAACGAACCAACGA+AGG + chr4.2:83171489-83171508 MS.gene99475:intron 45.0%
ACCAACGAAGGACACATACA+AGG + chr4.2:83171501-83171520 MS.gene99475:intron 45.0%
ACTGCTGATGCAAACTGGTT+TGG - chr4.2:83170629-83170648 None:intergenic 45.0%
AGCCCACAAAAATTGGCACT+GGG - chr4.2:83168500-83168519 None:intergenic 45.0%
AGGGTTCAACAACACTTGAG+GGG - chr4.2:83169722-83169741 None:intergenic 45.0%
ATCGATCTGTTCGGAGATCT+TGG - chr4.2:83171133-83171152 None:intergenic 45.0%
ATGGAAGCCACCAAAGAACT+AGG - chr4.2:83168070-83168089 None:intergenic 45.0%
CACTACTCATATACAAGGGG+GGG - chr4.2:83169842-83169861 None:intergenic 45.0%
CACTTGAGGGGAATTGCAAT+AGG - chr4.2:83169710-83169729 None:intergenic 45.0%
CAGGAACACCAAAGCAAGAA+AGG + chr4.2:83170993-83171012 MS.gene99475:CDS 45.0%
CCAAATTGTGGTCGCAATTG+TGG + chr4.2:83168678-83168697 MS.gene99475:intron 45.0%
CCACAATTGCGACCACAATT+TGG - chr4.2:83168681-83168700 None:intergenic 45.0%
CCACTACTCATATACAAGGG+GGG - chr4.2:83169843-83169862 None:intergenic 45.0%
CTTGGACTCTTTGTGGTGTA+CGG + chr4.2:83170859-83170878 MS.gene99475:CDS 45.0%
CTTTATCAGGATCAACCCTC+TGG - chr4.2:83169666-83169685 None:intergenic 45.0%
GACACCAGGATTGAGTAACA+CGG - chr4.2:83170894-83170913 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG + chr4.2:83168133-83168152 MS.gene99475:CDS 45.0%
GAGTTTGCAGAGTAAGACCA+TGG + chr4.2:83168396-83168415 MS.gene99475:intron 45.0%
GATCAACCCTCTGGATCAAT+AGG - chr4.2:83169657-83169676 None:intergenic 45.0%
GCCACTACTCATATACAAGG+GGG - chr4.2:83169844-83169863 None:intergenic 45.0%
GCTACTCGAACAGTAATGTC+TGG + chr4.2:83168951-83168970 MS.gene99475:intron 45.0%
GTGGAGAATTAGGTGTGAAC+TGG + chr4.2:83169317-83169336 MS.gene99475:intron 45.0%
GTGGTCTATGCGAGAAAGAA+TGG + chr4.2:83169471-83169490 MS.gene99475:intron 45.0%
TAACTCCACCAACATCTGCA+TGG - chr4.2:83168089-83168108 None:intergenic 45.0%
TAGAGGCTAAGCTGTACTTG+AGG - chr4.2:83170550-83170569 None:intergenic 45.0%
TAGCCCACAAAAATTGGCAC+TGG - chr4.2:83168501-83168520 None:intergenic 45.0%
TCCTTGTATGTGTCCTTCGT+TGG - chr4.2:83171505-83171524 None:intergenic 45.0%
TCTTGGATCCATTGCAGTCA+AGG - chr4.2:83171116-83171135 None:intergenic 45.0%
TGCAAAAGCCTTGACTGCAA+TGG + chr4.2:83171105-83171124 MS.gene99475:CDS 45.0%
! AGTGAGAACTGGGAGAGTTT+TGG + chr4.2:83169586-83169605 MS.gene99475:intron 45.0%
! ATGCAGATGTTGGTGGAGTT+AGG + chr4.2:83168088-83168107 MS.gene99475:CDS 45.0%
! ATTTGGATTCTGAGGGTCAG+GGG + chr4.2:83167954-83167973 MS.gene99475:CDS 45.0%
! CTCCCAGTGCCAATTTTTGT+GGG + chr4.2:83168495-83168514 MS.gene99475:intron 45.0%
! GAATTTGGATTCTGAGGGTC+AGG + chr4.2:83167952-83167971 MS.gene99475:CDS 45.0%
! GATTGTGCCTAGTTCTTTGG+TGG + chr4.2:83168060-83168079 MS.gene99475:CDS 45.0%
! GTGAGAACTGGGAGAGTTTT+GGG + chr4.2:83169587-83169606 MS.gene99475:intron 45.0%
! GTGTGAACTGGGAGAGTTTT+TGG + chr4.2:83169329-83169348 MS.gene99475:intron 45.0%
! TGCAGATGTTGGTGGAGTTA+GGG + chr4.2:83168089-83168108 MS.gene99475:CDS 45.0%
! TGGTTTGGACAACCAAACTG+TGG - chr4.2:83170920-83170939 None:intergenic 45.0%
! TTGCGACTGCAATTGCTGTT+TGG + chr4.2:83168796-83168815 MS.gene99475:intron 45.0%
!! AATTCCCTTTGGGGTTCGAT+AGG - chr4.2:83169742-83169761 None:intergenic 45.0%
!! ATTCCCTTTGGGGTTCGATA+GGG - chr4.2:83169741-83169760 None:intergenic 45.0%
!! CTGATGCAAACTGGTTTGGT+AGG - chr4.2:83170625-83170644 None:intergenic 45.0%
!! TGCGACTGCAATTGCTGTTT+GGG + chr4.2:83168797-83168816 MS.gene99475:intron 45.0%
AGGCTAAGCTGTACTTGAGG+AGG - chr4.2:83170547-83170566 None:intergenic 50.0%
AGTAAGACCATGGAGTGTGC+AGG + chr4.2:83168406-83168425 MS.gene99475:intron 50.0%
CCCCCCTTGTATATGAGTAG+TGG + chr4.2:83169840-83169859 MS.gene99475:intron 50.0%
CGACATCGCAACTGCAATTG+TGG - chr4.2:83168750-83168769 None:intergenic 50.0%
CTCTGACTGCTGATGCAAAC+TGG - chr4.2:83170634-83170653 None:intergenic 50.0%
GAAATCACTCCTCCCACTAC+AGG - chr4.2:83171061-83171080 None:intergenic 50.0%
GAACCCTATCGAACCCCAAA+GGG + chr4.2:83169735-83169754 MS.gene99475:intron 50.0%
GGGAATAGGGGTGGAGAATT+AGG + chr4.2:83169307-83169326 MS.gene99475:intron 50.0%
TAAGTAGGAGGGAATAGGGG+TGG + chr4.2:83169298-83169317 MS.gene99475:intron 50.0%
TATGAGGAATGTTGAGGGGC+TGG - chr4.2:83170940-83170959 None:intergenic 50.0%
TGAACCCTATCGAACCCCAA+AGG + chr4.2:83169734-83169753 MS.gene99475:intron 50.0%
TTGGTGAAGCCGAGGTAAAG+TGG + chr4.2:83171302-83171321 MS.gene99475:intron 50.0%
TTTAATTGGAGCCCCACAGG+TGG + chr4.2:83168542-83168561 MS.gene99475:intron 50.0%
! CCACAAAAATTGGCACTGGG+AGG - chr4.2:83168497-83168516 None:intergenic 50.0%
! CCTCCCAGTGCCAATTTTTG+TGG + chr4.2:83168494-83168513 MS.gene99475:intron 50.0%
! CTAGCTAGTCTGTACAGCTC+TGG + chr4.2:83168518-83168537 MS.gene99475:intron 50.0%
! GCACCGACACTTCTGATTGA+AGG + chr4.2:83168274-83168293 MS.gene99475:intron 50.0%
!! GATTAGCATTGGTGAAGCCG+AGG + chr4.2:83171294-83171313 MS.gene99475:CDS 50.0%
!! GTAGAAGTACCTGTAGTGGG+AGG + chr4.2:83171049-83171068 MS.gene99475:CDS 50.0%
!! GTGTCCTTCGTTGGTTCGTT+TGG - chr4.2:83171496-83171515 None:intergenic 50.0%
AAATCCCACCGTCCACCTGT+GGG - chr4.2:83168557-83168576 None:intergenic 55.0%
ACCATGGAGTGTGCAGGCTT+GGG + chr4.2:83168412-83168431 MS.gene99475:intron 55.0%
ACCTTGGAGTGTGCAGGCTT+GGG + chr4.2:83168435-83168454 MS.gene99475:intron 55.0%
GGCTTCCATGCAGATGTTGG+TGG + chr4.2:83168081-83168100 MS.gene99475:CDS 55.0%
GGTGGCTTCCATGCAGATGT+TGG + chr4.2:83168078-83168097 MS.gene99475:CDS 55.0%
TCTGAGGGTCAGGGGAATAG+TGG + chr4.2:83167962-83167981 MS.gene99475:CDS 55.0%
! CGCTCCGTGTTACTCAATCC+TGG + chr4.2:83170887-83170906 MS.gene99475:CDS 55.0%
! GGCTTTAATTGGAGCCCCAC+AGG + chr4.2:83168539-83168558 MS.gene99475:intron 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - chr4.2:83170935-83170954 None:intergenic 55.0%
AATCCCACCGTCCACCTGTG+GGG - chr4.2:83168556-83168575 None:intergenic 60.0%
AGTGTGCAGGCTTGGGACCT+TGG + chr4.2:83168419-83168438 MS.gene99475:intron 60.0%
ATTGGAGCCCCACAGGTGGA+CGG + chr4.2:83168546-83168565 MS.gene99475:intron 60.0%
CAAATCCCACCGTCCACCTG+TGG - chr4.2:83168558-83168577 None:intergenic 60.0%
CCAAACTGTGGTGCGACACC+AGG - chr4.2:83170908-83170927 None:intergenic 60.0%
CTTGGGACCTTGGAGTGTGC+AGG + chr4.2:83168429-83168448 MS.gene99475:intron 60.0%
GACCATGGAGTGTGCAGGCT+TGG + chr4.2:83168411-83168430 MS.gene99475:intron 60.0%
GACCTTGGAGTGTGCAGGCT+TGG + chr4.2:83168434-83168453 MS.gene99475:intron 60.0%
TCCCAAGCCTGCACACTCCA+AGG - chr4.2:83168416-83168435 None:intergenic 60.0%
TCCCAAGCCTGCACACTCCA+TGG - chr4.2:83168416-83168435 None:intergenic 60.0%
!! CCTGGTGTCGCACCACAGTT+TGG + chr4.2:83170905-83170924 MS.gene99475:CDS 60.0%
ACAAGGGGGGGAGCCCTTGA+AGG - chr4.2:83169830-83169849 None:intergenic 65.0%
GAGCCCCACAGGTGGACGGT+GGG + chr4.2:83168550-83168569 MS.gene99475:intron 70.0%
GGAGCCCCACAGGTGGACGG+TGG + chr4.2:83168549-83168568 MS.gene99475:intron 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83167782 83171848 83167782 ID=MS.gene99475
chr4.2 mRNA 83167782 83171848 83167782 ID=MS.gene99475.t1;Parent=MS.gene99475
chr4.2 exon 83167782 83168165 83167782 ID=MS.gene99475.t1.exon1;Parent=MS.gene99475.t1
chr4.2 CDS 83167782 83168165 83167782 ID=cds.MS.gene99475.t1;Parent=MS.gene99475.t1
chr4.2 exon 83170536 83171315 83170536 ID=MS.gene99475.t1.exon2;Parent=MS.gene99475.t1
chr4.2 CDS 83170536 83171315 83170536 ID=cds.MS.gene99475.t1;Parent=MS.gene99475.t1
chr4.2 exon 83171687 83171848 83171687 ID=MS.gene99475.t1.exon3;Parent=MS.gene99475.t1
chr4.2 CDS 83171687 83171848 83171687 ID=cds.MS.gene99475.t1;Parent=MS.gene99475.t1
Gene Sequence

>MS.gene99475

ATGTTGTTGAATCCGAAGAATCCATGTGAATCATTATGCAAGAAACTTTTCTTGAATATTGATGACACTGAATGGTCAAGTAAGTTTTTTGTGTGTGATGCTTGTAACAAGTTTACAACTTTTCAGAATCTTGATTGTACTTGTGGAAAACCCACTAATAGACAGCCTAAGAATTTGGATTCTGAGGGTCAGGGGAATAGTGGTTGTATTGCTGATGCTTTGAATGGGGTTTTTGTTAAGGAAAATGGGTCGTTGTTTTTGGTGTTTGATGATTTGAAGATTGTGCCTAGTTCTTTGGTGGCTTCCATGCAGATGTTGGTGGAGTTAGGGTATTCTGATTTGAGTCAATTAGAGGAAGTCACACACAACATTGGAAAACAGGAGGTAATGGTGTTGTCTTTTGCAATATGTATTTATCTCACTTTTTTTCTTTTTCTTTTTCATTTCAAGTTTTGAGTAAACCCGAGAATTGGGATGTCACATTACTCTGTTGCACCGACACTTCTGATTGAAGGTGTGTTTAGGTGTCTAACACATGTCGTGTACGACCCCAACACTTATGATTACATTGGATTATGTCATTTTCTCATAATTCTATTGACTTCATAATATGTGAGTTTGCAGAGTAAGACCATGGAGTGTGCAGGCTTGGGACCTTGGAGTGTGCAGGCTTGGGACCTTGGAGTATACTCTCAAAGTCTCGCATTCGAATCCTCCCAGTGCCAATTTTTGTGGGCTAGCTAGTCTGTACAGCTCTGGCTTTAATTGGAGCCCCACAGGTGGACGGTGGGATTTGTCTCCCAAATTAGTTGGTCCTTGAACTGCATACTGAGTTTTGAAAAAAAACTGTAGGTTAAGAAAATTCAAAAAAATATTTGAAACTATGAATCATTGTTCCAAATTGTGGTCGCAATTGTGGCTGCGATGCGAACCTTGATATTGTAGTAAAATGACGGTAAATGCAACCACAATTGCAGTTGCGATGTCGTTGCAGAGACCTCTAAAACCTTTATATTGCGACTGCAATTGCTGTTTGGGACTGCAACTTAGAACTATGTTGTTAATTGTTGGTTGTTTCAGTCTAAGATTATTTGGGACTCAGATCTTAAGCGTTATCATTTTAGTCTAGGCTTAGTTAGAATTCTGGTGTTGACTATGAGTTTCAAAATGCTACTCGAACAGTAATGTCTGGATCAAAATACCTGACGATTAATGAGTTCGAGATCTTTTCTAATTTTTTTTCTTGTTTCACAAACAATTTTAACAATGTCCTATAATTTATTGTTTGAACTTTGAACTTGCATTGGATCTTTAGACCATTCTTGAATTATGAACTTTCAACCTATATAACTTGATGCTTTTAGGGTAGGATGTATTTGGCGTAGTATCTGTTAAGAAGCTGATAGAAGAGCATTTATTAAGAAAGCCTATGTGAGAAAGAATATAAAGGAAAGTAAGGAGGGAAATAAATTGGTAATAACCGATAAGCTGCAAACTAACAGAAAAAATGATGTAATAAGTAGGAGGGAATAGGGGTGGAGAATTAGGTGTGAACTGGGAGAGTTTTTGGTGCATACTCTGAATCTTTAGATCGTACTTGAATTATGAATCTTTGTCCTAAAACTTAAGATAGTTTGGATTTGACATAGTAAGGGACATCTGTTAAGAACCTCATAAGAGTGCATTAAGGTGGTCTATGCGAGAAAGAATGGAAAGGAAAATAAGTAGGGAAATCGAGTGGTTGTTGTAACTGATATAGCTGAGGGTAACAGAATGTAATAAGTAAGGGACTCTTACAGAAGTAAGTGAGAACTGGGAGAGTTTTGGGCCACTCTTAAATTAGCCTGCAGTTTATAATTTTTTTCCTTTGATCCTATTGATCCAGAGGGTTGATCCTGATAAAGTTCATCTTTTTCCATTGAATTCCTATTGCAATTCCCCTCAAGTGTTGTTGAACCCTATCGAACCCCAAAGGGAATTAATTGGGTAAAAGAGAATATGGTTTTTACTGATTGAATTAAACAAAACCAAAGAGAAAGATTTCTCCTTCAAGGGCTCCCCCCCTTGTATATGAGTAGTGGCTCTCTCTCTTTGATCAATACTAACATTTGCCATTAAAATAAACTTGGGGTCCGTGTACTTTGTGAAAACATTTACTATTTTCATTTCCTAAAATGTGTTAGTTGTATTTATTAACCAAGGAAATGTGTTGGTTGTAGTTATTAACAAGGAAAATGTGTCGGTTGTATTTGTTAACAAGGAAAAAAAAGAATCTTGAATTAAACAATTGTCTTGCATTTCAAGAAAAATGAAAATATCGAAAAAACTATTTTCATTATTTCCTGAAATGCATCATAGCTAGCATTTTATCTTATCTCCATCACAAAGACTCACTTTAAAAGTCTTTTTTCTCCTTATTAACAAGTAAACCTAAGACCTCTTGAAATAAAGAGCAAGTAAAACTAACACAAATTCTATAAAATGAAAATAGAAAATGGTTTCACAAAATTAAACGGGTCCTTACCTTAATGGCTCATTGTTTAATTAGTGTTTTTGTCATTGGCTTCAGACGAATGTTTCCTCAAACACTCCTTAGAGTAACTTTGGTATCAACCGTGCATTGAGATGTGTACGAACACCTACACAAACACACGTGCTGTTATTCTTACCGTTTCGTTTTAACATCATGTATATATGCCAAGTAATGTAAACTTGTTTGTGAAATATTTTAACAAATACAATTTTGATGTACAGATGTTGAGCCTCCTCAAGTACAGCTTAGCCTCTAATGAGCCATTGACAAATACAATTTTGAAAAGCAGCTCCAAGAAGAAAGATAACCTACCAAACCAGTTTGCATCAGCAGTCAGAGTAAAAACATGCACCCGTGACATTAAAATGGATGTTAAGCTAGTGCGAAGTAAATCTCAGAAAAAGATAATCTTTGCAGAAACCAGTGAAAACTTTGTTGACTTCATGTTTAGCTTCCTTACAATACCATTAGGGTCCATTGTAAAGGTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGAGTGTAGAGAACTTGGACTCTTTGTGGTGTACGGACTTGCGCTCCGTGTTACTCAATCCTGGTGTCGCACCACAGTTTGGTTGTCCAAACCAGCCCCTCAACATTCCTCATATACAAACACTCACCACTACATATTATTATGGTACAGGAACACCAAAGCAAGAAAGGGATTTACGCTATAGTAGTGCCCGTTATAATACAGTAGAAGTACCTGTAGTGGGAGGAGTGATTTCTAAGACAATGGGATCAATTTATAATGCAAAAGCCTTGACTGCAATGGATCCAAGATCTCCGAACAGATCGATTAAAGATGTGGTGGGATTTGTAAAGAGACCAGCATTATATGTTGTAGGAGATGATCTAAAAGTAAAATCACTTTCTGCTAACTCTTGGCTTTCATATTTGAAAGAATTGAGCTTTCCCCTTGATGATCTTGAAGTGAAGGTGATTAGCATTGGTGAAGCCGAGGTAAAGTGGCTATTACTACTGAATTTATCATTTTTTTTTTCTTTTTAGTAAACAATCATGTTGGTCCTTAAATGTGAGATGGTGTCACTATAGTCTGAATGTATTAATATATCATAAAAACATCCCTGAATGTGTCTTGTGTTAGTAATTTTATCAACCAAACTGATTAACAGAAGACCAAACGAACCAACGAAGGACACATACAAGGATTAAACTGAATAAGAAAACACAAATTCTTGTATGTTTTTTTAGTTTTGAAAGATTCATGAATTATAATGACAGTACTCACACTTTTAAGGACCTGATTAAGTACTCTTTTGTTTTGTTTTCTTTGTTGTTGATACTCTTCCTTTATGGTTTGCACTTTGCAGGCTCTGAGCCTCCTAGCAGCTTCTTTGATATCAAAATCTACACTGACTAGTGGACTAGAAGAATTTTTTAATTTGCAAAAACAAGATTCAAATTTGATCTCAAAGTGTGTTGAAACTAGTAGGGTTGATAAAGTTGCAAAGAAGCCTAAACTTGTACACTGA
Protein sequence

>MS.gene99475.t1

MLLNPKNPCESLCKKLFLNIDDTEWSSKFFVCDACNKFTTFQNLDCTCGKPTNRQPKNLDSEGQGNSGCIADALNGVFVKENGSLFLVFDDLKIVPSSLVASMQMLVELGYSDLSQLEEVTHNIGKQEMLSLLKYSLASNEPLTNTILKSSSKKKDNLPNQFASAVRVKTCTRDIKMDVKLVRSKSQKKIIFAETSENFVDFMFSFLTIPLGSIVKVLDGNSFVGCVDNLYKSVENLDSLWCTDLRSVLLNPGVAPQFGCPNQPLNIPHIQTLTTTYYYGTGTPKQERDLRYSSARYNTVEVPVVGGVISKTMGSIYNAKALTAMDPRSPNRSIKDVVGFVKRPALYVVGDDLKVKSLSANSWLSYLKELSFPLDDLEVKVISIGEAEALSLLAASLISKSTLTSGLEEFFNLQKQDSNLISKCVETSRVDKVAKKPKLVH