AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037551

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037551.t1 MTR_4g017610 66.739 463 148 5 2 462 3 461 0.0 595
MS.gene037551.t1 MTR_4g017410 65.328 473 123 8 10 458 7 462 0.0 583
MS.gene037551.t1 MTR_4g017440 65.106 470 142 5 10 458 12 480 0.0 575
MS.gene037551.t1 MTR_4g017830 61.077 483 159 9 1 458 1 479 0.0 556
MS.gene037551.t1 MTR_4g017550 64.978 454 133 10 26 458 3 451 0.0 546
MS.gene037551.t1 MTR_4g019340 60.714 476 142 11 9 458 35 491 0.0 529
MS.gene037551.t1 MTR_4g017470 57.613 486 140 9 1 458 1 448 2.52e-172 493
MS.gene037551.t1 MTR_4g019310 40.385 468 248 13 13 458 9 467 1.92e-99 307
MS.gene037551.t1 MTR_4g019310 34.343 99 51 2 269 354 8 105 1.96e-11 66.2
MS.gene037551.t1 MTR_7g066350 34.483 464 238 12 13 457 10 426 5.43e-71 232
MS.gene037551.t1 MTR_7g066350 28.780 205 120 6 265 451 5 201 6.47e-13 70.9
MS.gene037551.t1 MTR_7g066570 36.518 471 239 19 13 454 15 454 4.21e-70 233
MS.gene037551.t1 MTR_7g066340 34.335 466 237 16 13 457 13 430 9.18e-68 224
MS.gene037551.t1 MTR_7g066340 28.571 203 117 7 264 447 7 200 9.17e-14 73.6
MS.gene037551.t1 MTR_7g066340 25.610 246 139 7 10 252 261 465 2.98e-11 65.9
MS.gene037551.t1 MTR_7g058710 31.687 486 247 16 13 457 13 454 1.52e-62 211
MS.gene037551.t1 MTR_7g066260 33.898 472 231 18 13 454 16 436 2.46e-62 210
MS.gene037551.t1 MTR_7g066260 28.774 212 122 6 269 458 15 219 1.98e-12 69.3
MS.gene037551.t1 MTR_1g041315 33.405 467 252 14 13 457 15 444 4.40e-62 209
MS.gene037551.t1 MTR_7g012680 33.839 461 230 16 13 454 15 419 3.27e-60 204
MS.gene037551.t1 MTR_7g012680 26.728 217 117 6 261 440 6 217 1.82e-11 66.2
MS.gene037551.t1 MTR_7g066410 31.485 505 276 16 13 463 11 499 4.98e-58 199
MS.gene037551.t1 MTR_7g066360 35.242 454 237 15 13 454 15 423 3.12e-56 194
MS.gene037551.t1 MTR_7g066360 26.606 218 134 6 260 458 5 215 7.29e-11 64.7
MS.gene037551.t1 MTR_7g063900 32.558 473 265 17 11 454 9 456 3.98e-56 194
MS.gene037551.t1 MTR_7g066580 31.799 478 248 15 18 463 1 432 5.61e-54 187
MS.gene037551.t1 MTR_7g066430 30.608 477 288 12 9 457 1 462 2.24e-53 187
MS.gene037551.t1 MTR_7g063890 31.210 471 269 16 13 454 28 472 7.71e-52 183
MS.gene037551.t1 MTR_7g063890 29.798 198 108 6 264 441 22 208 2.18e-11 66.2
MS.gene037551.t1 MTR_7g063660 31.263 467 265 17 13 454 10 445 1.59e-51 181
MS.gene037551.t1 MTR_7g063660 31.461 178 101 6 268 431 8 178 5.91e-14 74.3
MS.gene037551.t1 MTR_7g066290 32.009 453 236 12 13 454 16 407 4.04e-51 179
MS.gene037551.t1 MTR_7g066400 31.169 462 235 17 13 454 13 411 1.51e-50 178
MS.gene037551.t1 MTR_7g062280 30.361 471 277 14 13 454 11 459 1.03e-49 177
MS.gene037551.t1 MTR_7g063840 30.297 472 270 17 5 454 7 441 8.00e-48 171
MS.gene037551.t1 MTR_7g063880 30.603 464 261 16 13 454 12 436 8.91e-48 171
MS.gene037551.t1 MTR_7g066480 31.102 463 230 13 13 462 17 403 8.06e-47 167
MS.gene037551.t1 MTR_7g062300 29.303 488 276 17 1 457 1 450 3.15e-46 167
MS.gene037551.t1 MTR_7g058700 29.095 464 207 12 22 457 529 898 4.47e-45 169
MS.gene037551.t1 MTR_7g063830 31.798 456 235 18 13 437 11 421 4.97e-44 160
MS.gene037551.t1 MTR_7g066310 31.330 466 186 16 13 457 8 360 3.19e-42 154
MS.gene037551.t1 MTR_2g029400 29.274 468 212 13 13 454 15 389 5.00e-42 154
MS.gene037551.t1 MTR_4g019240 61.789 123 44 2 1 122 1 121 1.04e-41 147
MS.gene037551.t1 MTR_7g063820 32.365 482 246 20 13 454 11 452 1.77e-41 154
MS.gene037551.t1 MTR_3g065200 29.474 475 245 17 13 465 4 410 5.84e-41 152
MS.gene037551.t1 MTR_7g066490 28.603 451 243 9 13 462 17 389 3.35e-39 147
MS.gene037551.t1 MTR_7g063670 31.277 470 251 20 3 454 10 425 2.00e-38 145
MS.gene037551.t1 MTR_3g007740 29.892 465 259 18 9 457 6 419 3.40e-38 144
MS.gene037551.t1 MTR_7g063920 28.035 453 255 12 13 454 12 404 4.56e-38 144
MS.gene037551.t1 MTR_1g014060 28.352 455 200 18 21 454 9 358 1.22e-32 128
MS.gene037551.t1 MTR_7g066680 36.792 212 102 7 268 458 85 285 1.93e-25 107
MS.gene037551.t1 MTR_7g066280 26.485 404 215 12 99 457 1 367 1.02e-24 106
MS.gene037551.t1 MTR_7g062270 35.000 200 98 10 13 200 11 190 2.31e-19 90.1
MS.gene037551.t1 MTR_8g445860 27.755 245 143 7 10 240 11 235 1.11e-16 79.7
MS.gene037551.t1 MTR_8g445860 26.923 208 122 5 269 454 13 212 2.08e-14 73.2
MS.gene037551.t1 MTR_8g023240 23.782 349 221 11 12 345 37 355 3.06e-16 81.6
MS.gene037551.t1 MTR_7g063720 29.500 200 105 5 269 454 23 200 3.54e-16 78.2
MS.gene037551.t1 MTR_1g115440 27.197 239 149 7 13 240 12 236 5.34e-15 74.7
MS.gene037551.t1 MTR_6g006800 62.903 62 12 2 1 62 1 51 1.86e-13 65.5
MS.gene037551.t1 MTR_4g019330 30.058 173 71 4 283 454 32 155 2.89e-13 68.6
MS.gene037551.t1 MTR_0150s0010 28.750 240 144 8 13 240 11 235 5.23e-13 68.9
MS.gene037551.t1 MTR_7g100420 28.627 255 145 10 220 454 134 371 1.28e-12 69.7
MS.gene037551.t1 MTR_7g100420 67.442 43 14 0 12 54 39 81 2.10e-11 65.9
MS.gene037551.t1 MTR_1g115455 35.252 139 71 5 13 145 10 135 5.56e-12 65.9
MS.gene037551.t1 MTR_1g115435 27.602 221 120 8 10 225 5 190 1.21e-11 64.7
MS.gene037551.t1 MTR_1g115450 37.975 79 41 1 269 339 7 85 1.75e-11 62.4
MS.gene037551.t1 MTR_0150s0030 32.000 100 53 2 269 359 8 101 7.68e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037551.t1 AT5G01120 34.105 475 267 16 13 458 10 467 3.36e-65 218
MS.gene037551.t1 AT5G43240 33.333 486 260 19 13 458 10 471 1.18e-56 196
MS.gene037551.t1 AT5G43240 33.333 486 260 19 13 458 10 471 1.18e-56 196
MS.gene037551.t1 AT5G43240 33.333 486 260 19 13 458 10 471 1.18e-56 196
MS.gene037551.t1 AT5G01130 32.965 452 256 16 13 457 10 421 1.45e-54 189
MS.gene037551.t1 AT3G09140 30.328 488 278 18 10 458 7 471 1.18e-50 179
MS.gene037551.t1 AT5G01150 32.334 467 272 13 15 458 12 457 5.48e-50 177
MS.gene037551.t1 AT3G09110 35.061 328 180 11 15 330 12 318 5.24e-48 168
MS.gene037551.t1 AT3G09140 28.738 515 277 19 10 457 7 498 1.45e-45 166
MS.gene037551.t1 AT5G01140 30.853 457 256 15 15 454 12 425 3.22e-45 164
MS.gene037551.t1 AT3G09120 33.434 332 181 11 10 323 4 313 3.27e-43 155
MS.gene037551.t1 AT5G37320 29.067 461 285 17 19 458 18 457 2.16e-35 137
MS.gene037551.t1 AT5G43240 30.422 332 185 11 160 458 9 327 1.75e-27 113

Find 92 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTATTACAAATACAATTT+TGG 0.077846 4.3:+81951836 MS.gene037551:CDS
CATCGTCTGAGTTGAGATTT+TGG 0.163224 4.3:-81950849 None:intergenic
CCCAAAATTGTATTTGTAAT+AGG 0.221679 4.3:-81951837 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.255481 4.3:-81951051 None:intergenic
TAATAGACAACCTAAGAATT+TGG 0.261898 4.3:+81951053 MS.gene037551:CDS
GTGGGTTGTGGTATTAAATA+AGG 0.297283 4.3:-81952200 None:intergenic
CTCTAACTACTGATGCAAAT+TGG 0.299041 4.3:-81951893 None:intergenic
AGCTTCCTTACCATGCCATT+GGG 0.310690 4.3:+81952029 MS.gene037551:CDS
TGTTGTCAGTGGTGGCTAAA+AGG 0.330255 4.3:-81950759 None:intergenic
ATCTCAACTCAGACGATGTT+TGG 0.331962 4.3:+81950854 MS.gene037551:CDS
CTTAGAGATGTTGGTTCCAT+TGG 0.338874 4.3:+81951185 MS.gene037551:CDS
CCACAACCCACGTATTATTA+TGG 0.345905 4.3:+81952212 MS.gene037551:CDS
GTGAAGAGAGCAACATTATA+TGG 0.349402 4.3:+81952365 MS.gene037551:CDS
CGGCCCGTATCCAAATTCTT+AGG 0.353895 4.3:-81951063 None:intergenic
AGGCAAAACTTAGCAGAAAC+TGG 0.372974 4.3:-81952413 None:intergenic
AATTCTTCACAAGGATTCCT+TGG 0.390278 4.3:-81950910 None:intergenic
GGAATGTTGAGGGGCTGGTT+TGG 0.392660 4.3:-81952179 None:intergenic
TAGCTTCCTTACCATGCCAT+TGG 0.394737 4.3:+81952028 MS.gene037551:CDS
AATCCCACAGCGCCTTCTCT+TGG 0.395694 4.3:-81952341 None:intergenic
CAATGGAACCAACATCTCTA+AGG 0.402119 4.3:-81951184 None:intergenic
GTACAAGAGTGTTCAGAACT+TGG 0.408992 4.3:+81952106 MS.gene037551:CDS
ACTGATGCAAATTGGTTAGC+TGG 0.412013 4.3:-81951885 None:intergenic
TTAGAGATGTTGGTTCCATT+GGG 0.416438 4.3:+81951186 MS.gene037551:CDS
AATAAGGAATGTTGAGGGGC+TGG 0.425217 4.3:-81952184 None:intergenic
AATTCCCCTCAAAATCTTAG+TGG 0.429720 4.3:+81950639 MS.gene037551:CDS
AAGAATTTGGATACGGGCCG+GGG 0.431114 4.3:+81951066 MS.gene037551:CDS
CATTGGTGAATTTGAGGTAA+AGG 0.432182 4.3:+81952493 MS.gene037551:CDS
ATCCCACAGCGCCTTCTCTT+GGG 0.434305 4.3:-81952340 None:intergenic
TAAGAATTTGGATACGGGCC+GGG 0.434440 4.3:+81951065 MS.gene037551:CDS
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.3:+81950724 MS.gene037551:CDS
GTTGAGTTCCTTAGAGATGT+TGG 0.445404 4.3:+81951176 MS.gene037551:CDS
GGATTAAGTAACACAGAAGT+AGG 0.447147 4.3:-81952131 None:intergenic
ACTTCTGTGTTACTTAATCC+TGG 0.451739 4.3:+81952134 MS.gene037551:CDS
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.3:-81950729 None:intergenic
GATAAGCATTGGTGAATTTG+AGG 0.462994 4.3:+81952487 MS.gene037551:CDS
CAAAACCTTGATTGCACCTG+TGG 0.476027 4.3:+81951021 MS.gene037551:CDS
CTGGTATAGCACCACAACTT+AGG 0.481431 4.3:+81952153 MS.gene037551:CDS
GACTATTATCCTACAAAAGA+AGG 0.481572 4.3:+81952260 MS.gene037551:CDS
TTCAAGGTCGTTGATTGCAC+TGG 0.491294 4.3:+81952316 MS.gene037551:CDS
AAGGAACTCAACATGGAACT+TGG 0.499089 4.3:-81951165 None:intergenic
AACAAATGTTGCTGCGTCCA+AGG 0.504062 4.3:+81950893 MS.gene037551:CDS
GGAGTCTTCGTTAGAGAAAG+TGG 0.504992 4.3:+81951105 MS.gene037551:CDS
CCATAATAATACGTGGGTTG+TGG 0.507333 4.3:-81952212 None:intergenic
CTAAGTTGTGGTGCTATACC+AGG 0.515459 4.3:-81952152 None:intergenic
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4.3:-81951048 None:intergenic
CATCTCTAAGGAACTCAACA+TGG 0.531180 4.3:-81951172 None:intergenic
ACACATCCAGCAAAAGAGTC+AGG 0.540251 4.3:-81952074 None:intergenic
ATGACTCTGAGAGTTGTTGT+AGG 0.542216 4.3:-81950786 None:intergenic
TGGTAAAATCTTCAAATCAT+CGG 0.545386 4.3:-81951145 None:intergenic
AGAGATATTTGATGTTGTCA+AGG 0.547142 4.3:-81950816 None:intergenic
CTGATGCAAATTGGTTAGCT+GGG 0.547375 4.3:-81951884 None:intergenic
CTGTCGACCATAATAATACG+TGG 0.548759 4.3:-81952219 None:intergenic
ACAACCTAAGAATTTGGATA+CGG 0.549258 4.3:+81951059 MS.gene037551:CDS
TCTGTTCTTTCAGATATGAA+AGG 0.549535 4.3:-81952433 None:intergenic
TTGTAGGTCATTGTTGTCAG+TGG 0.554864 4.3:-81950770 None:intergenic
TATTAAATAAGGAATGTTGA+GGG 0.558962 4.3:-81952189 None:intergenic
TCTTGACGATCTTGAAGTGA+AGG 0.559099 4.3:+81952463 MS.gene037551:CDS
TAGGTCATTGTTGTCAGTGG+TGG 0.559820 4.3:-81950767 None:intergenic
CTAAGAATTTGGATACGGGC+CGG 0.563009 4.3:+81951064 MS.gene037551:CDS
CACTTGAGGCATAATTCCCC+CGG 0.572600 4.3:-81951083 None:intergenic
TGGACCCCAATGGCATGGTA+AGG 0.574831 4.3:-81952034 None:intergenic
TACAATGGACCCCAATGGCA+TGG 0.580817 4.3:-81952039 None:intergenic
GCTTCCTTACCATGCCATTG+GGG 0.583133 4.3:+81952030 MS.gene037551:CDS
GACCCAAGAGAAGGCGCTGT+GGG 0.589843 4.3:+81952338 MS.gene037551:CDS
TTCATGCATAATTCTTCACA+AGG 0.597426 4.3:-81950919 None:intergenic
GTATTAAATAAGGAATGTTG+AGG 0.602215 4.3:-81952190 None:intergenic
TATTTGATGTTGTCAAGGAA+TGG 0.603436 4.3:-81950811 None:intergenic
AGAATTTGGATACGGGCCGG+GGG 0.604267 4.3:+81951067 MS.gene037551:CDS
CAACCTAAGAATTTGGATAC+GGG 0.615212 4.3:+81951060 MS.gene037551:CDS
TGGTTTGGACACCTAAGTTG+TGG 0.615467 4.3:-81952164 None:intergenic
GATTAAGTAACACAGAAGTA+GGG 0.615768 4.3:-81952130 None:intergenic
TTTCTGATATCATCGGGACA+AGG 0.617619 4.3:-81951921 None:intergenic
GAAGAAGTCACACACAACAT+TGG 0.621785 4.3:+81951231 MS.gene037551:CDS
ATTGCACTGGACCCAAGAGA+AGG 0.623682 4.3:+81952329 MS.gene037551:CDS
GTCAAGTCTGCATAACCCAA+TGG 0.629435 4.3:-81951201 None:intergenic
ATTAAATAAGGAATGTTGAG+GGG 0.631161 4.3:-81952188 None:intergenic
AGAGCAACATTATATGGTGT+AGG 0.631695 4.3:+81952371 MS.gene037551:CDS
ATAATCTATGCAGAAGCGAA+CGG 0.635454 4.3:+81951984 MS.gene037551:CDS
ACACAACATTGGCAAGCAAG+AGG 0.636713 4.3:+81951242 MS.gene037551:CDS
AGGTCCTAAAAGCTTTACAA+TGG 0.639517 4.3:-81952054 None:intergenic
TTCACTTCAAGATCGTCAAG+AGG 0.643323 4.3:-81952461 None:intergenic
TCTAACGAAGACTCCACTTG+AGG 0.644921 4.3:-81951097 None:intergenic
AGGCGAAGATATACTTGAGG+AGG 0.647256 4.3:-81951806 None:intergenic
GAGAGGCGAAGATATACTTG+AGG 0.649287 4.3:-81951809 None:intergenic
GAAGTGAAGGTGATAAGCAT+TGG 0.654807 4.3:+81952476 MS.gene037551:CDS
ATTTGTAATAGGCTCATGAG+AGG 0.655380 4.3:-81951826 None:intergenic
AGCTTTACAATGGACCCCAA+TGG 0.659874 4.3:-81952044 None:intergenic
GGACCCAAGAGAAGGCGCTG+TGG 0.662998 4.3:+81952337 MS.gene037551:CDS
GGTGGCTAAAAGGCGAACGA+TGG 0.684330 4.3:-81950749 None:intergenic
TGTCGACCATAATAATACGT+GGG 0.685398 4.3:-81952218 None:intergenic
GTTGTTGAATGTTGATGACA+CGG 0.700176 4.3:+81950945 MS.gene037551:CDS
AGGCGAACGATGGTACCAAG+TGG 0.729828 4.3:-81950739 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAGAATATATTAAAAA+AGG + chr4.3:81951549-81951568 MS.gene037551:intron 10.0%
!!! TTTTTAATAGTATTTTACCT+AGG + chr4.3:81951690-81951709 MS.gene037551:intron 15.0%
!! ATTGTAAGAATAACAGTTTA+AGG - chr4.3:81951755-81951774 None:intergenic 20.0%
!! CTATACAAAATGTTAAAACA+TGG - chr4.3:81951778-81951797 None:intergenic 20.0%
!! TATTAAATAAGGAATGTTGA+GGG - chr4.3:81952192-81952211 None:intergenic 20.0%
!!! ATGAACTAATTTTAAGTTCA+AGG + chr4.3:81952300-81952319 MS.gene037551:CDS 20.0%
!!! CCTATTACAAATACAATTTT+GGG + chr4.3:81951837-81951856 MS.gene037551:CDS 20.0%
!!! TATCTCTAAAAACATTATTG+CGG + chr4.3:81951405-81951424 MS.gene037551:intron 20.0%
! AATAAGAGAAGAAACATGTT+AGG + chr4.3:81951595-81951614 MS.gene037551:intron 25.0%
! ATCTTTATTGTGGATAAATG+CGG + chr4.3:81951358-81951377 MS.gene037551:intron 25.0%
! ATTAAATAAGGAATGTTGAG+GGG - chr4.3:81952191-81952210 None:intergenic 25.0%
! CCCAAAATTGTATTTGTAAT+AGG - chr4.3:81951840-81951859 None:intergenic 25.0%
! GTAAGAAAAAGTCAATGTAA+TGG - chr4.3:81951273-81951292 None:intergenic 25.0%
! GTATTAAATAAGGAATGTTG+AGG - chr4.3:81952193-81952212 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG + chr4.3:81951053-81951072 MS.gene037551:CDS 25.0%
! TATGTTGTGAATCTTTATTG+TGG + chr4.3:81951348-81951367 MS.gene037551:intron 25.0%
! TGGTAAAATCTTCAAATCAT+CGG - chr4.3:81951148-81951167 None:intergenic 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG - chr4.3:81951054-81951073 None:intergenic 25.0%
!! ATGCTAGCCAAAAAAATTTT+TGG - chr4.3:81951719-81951738 None:intergenic 25.0%
!! GCCTATTACAAATACAATTT+TGG + chr4.3:81951836-81951855 MS.gene037551:CDS 25.0%
!! TGCTAGCCAAAAAAATTTTT+GGG - chr4.3:81951718-81951737 None:intergenic 25.0%
!!! AAAAAAATTTTTGGGAACCT+AGG - chr4.3:81951710-81951729 None:intergenic 25.0%
!!! ATCCATTTTTCTGATATCAT+CGG - chr4.3:81951931-81951950 None:intergenic 25.0%
!!! GTTTTTAGAGATATTCACAT+CGG - chr4.3:81951398-81951417 None:intergenic 25.0%
!!! TAGGTTCCCAAAAATTTTTT+TGG + chr4.3:81951709-81951728 MS.gene037551:intron 25.0%
!!! TGACTTTTTCTTACTTACAT+AGG + chr4.3:81951279-81951298 MS.gene037551:intron 25.0%
AAGAAACATGTTAGGATAAG+AGG + chr4.3:81951603-81951622 MS.gene037551:intron 30.0%
ACAACCTAAGAATTTGGATA+CGG + chr4.3:81951059-81951078 MS.gene037551:CDS 30.0%
AGAGATATTTGATGTTGTCA+AGG - chr4.3:81950819-81950838 None:intergenic 30.0%
ATAAGTCTGCTAGTAATATC+AGG + chr4.3:81951484-81951503 MS.gene037551:intron 30.0%
GACTATTATCCTACAAAAGA+AGG + chr4.3:81952260-81952279 MS.gene037551:CDS 30.0%
GATTAAGTAACACAGAAGTA+GGG - chr4.3:81952133-81952152 None:intergenic 30.0%
TATTTGATGTTGTCAAGGAA+TGG - chr4.3:81950814-81950833 None:intergenic 30.0%
TCATAAGTTAGCATAATTGC+AGG - chr4.3:81951512-81951531 None:intergenic 30.0%
TCTGTTCTTTCAGATATGAA+AGG - chr4.3:81952436-81952455 None:intergenic 30.0%
TCTTACTTACATAGGATCTT+AGG + chr4.3:81951287-81951306 MS.gene037551:intron 30.0%
TTCATGCATAATTCTTCACA+AGG - chr4.3:81950922-81950941 None:intergenic 30.0%
! AGAAAGTGGACAAAAGTTTT+TGG + chr4.3:81951119-81951138 MS.gene037551:CDS 30.0%
! AGTTGAGATTTTGGACAATT+TGG - chr4.3:81950843-81950862 None:intergenic 30.0%
! GAAATCAATCCTTCTTTTGT+AGG - chr4.3:81952272-81952291 None:intergenic 30.0%
! TAGGATAAGAGGTATAGTTA+GGG + chr4.3:81951614-81951633 MS.gene037551:intron 30.0%
! TTAGGATAAGAGGTATAGTT+AGG + chr4.3:81951613-81951632 MS.gene037551:intron 30.0%
! TTTGTCCACTAAGATTTTGA+GGG - chr4.3:81950647-81950666 None:intergenic 30.0%
!! TCCATTTTTCTGATATCATC+GGG - chr4.3:81951930-81951949 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG + chr4.3:81950669-81950688 MS.gene037551:CDS 30.0%
!!! ATTTTTTTGGCTAGCATTAG+TGG + chr4.3:81951722-81951741 MS.gene037551:intron 30.0%
AATTCCCCTCAAAATCTTAG+TGG + chr4.3:81950639-81950658 MS.gene037551:CDS 35.0%
AATTCTTCACAAGGATTCCT+TGG - chr4.3:81950913-81950932 None:intergenic 35.0%
AGAGCAACATTATATGGTGT+AGG + chr4.3:81952371-81952390 MS.gene037551:CDS 35.0%
AGGTCCTAAAAGCTTTACAA+TGG - chr4.3:81952057-81952076 None:intergenic 35.0%
AGTTAGGGAAGTTAAGTAGT+TGG + chr4.3:81951629-81951648 MS.gene037551:intron 35.0%
ATAACTGAAACCTCAAGTGA+GGG - chr4.3:81951331-81951350 None:intergenic 35.0%
ATAATCTATGCAGAAGCGAA+CGG + chr4.3:81951984-81952003 MS.gene037551:CDS 35.0%
ATTTGTAATAGGCTCATGAG+AGG - chr4.3:81951829-81951848 None:intergenic 35.0%
CAACCTAAGAATTTGGATAC+GGG + chr4.3:81951060-81951079 MS.gene037551:CDS 35.0%
CTCTAACTACTGATGCAAAT+TGG - chr4.3:81951896-81951915 None:intergenic 35.0%
GGATTAAGTAACACAGAAGT+AGG - chr4.3:81952134-81952153 None:intergenic 35.0%
GTGAAGAGAGCAACATTATA+TGG + chr4.3:81952365-81952384 MS.gene037551:CDS 35.0%
TAGCAGACTTATATCCCAAA+GGG - chr4.3:81951476-81951495 None:intergenic 35.0%
TCCCGATGATATCAGAAAAA+TGG + chr4.3:81951926-81951945 MS.gene037551:CDS 35.0%
TGTCGACCATAATAATACGT+GGG - chr4.3:81952221-81952240 None:intergenic 35.0%
! CATTGGTGAATTTGAGGTAA+AGG + chr4.3:81952493-81952512 MS.gene037551:CDS 35.0%
! GTGGGTTGTGGTATTAAATA+AGG - chr4.3:81952203-81952222 None:intergenic 35.0%
! GTTTGTCCACTAAGATTTTG+AGG - chr4.3:81950648-81950667 None:intergenic 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG - chr4.3:81951051-81951070 None:intergenic 35.0%
! TTGTCCACTAAGATTTTGAG+GGG - chr4.3:81950646-81950665 None:intergenic 35.0%
!! ACTTCTGTGTTACTTAATCC+TGG + chr4.3:81952134-81952153 MS.gene037551:CDS 35.0%
!! GATAAGCATTGGTGAATTTG+AGG + chr4.3:81952487-81952506 MS.gene037551:CDS 35.0%
!! GTTGTTGAATGTTGATGACA+CGG + chr4.3:81950945-81950964 MS.gene037551:CDS 35.0%
!! TTAGAGATGTTGGTTCCATT+GGG + chr4.3:81951186-81951205 MS.gene037551:CDS 35.0%
AAGGAACTCAACATGGAACT+TGG - chr4.3:81951168-81951187 None:intergenic 40.0%
ACTGATGCAAATTGGTTAGC+TGG - chr4.3:81951888-81951907 None:intergenic 40.0%
AGAACTATGCTTCACCCTTT+GGG + chr4.3:81951459-81951478 MS.gene037551:intron 40.0%
AGGCAAAACTTAGCAGAAAC+TGG - chr4.3:81952416-81952435 None:intergenic 40.0%
ATCTCAACTCAGACGATGTT+TGG + chr4.3:81950854-81950873 MS.gene037551:CDS 40.0%
CAATGGAACCAACATCTCTA+AGG - chr4.3:81951187-81951206 None:intergenic 40.0%
CATAACTGAAACCTCAAGTG+AGG - chr4.3:81951332-81951351 None:intergenic 40.0%
CCACAACCCACGTATTATTA+TGG + chr4.3:81952212-81952231 MS.gene037551:CDS 40.0%
CTAGCAGACTTATATCCCAA+AGG - chr4.3:81951477-81951496 None:intergenic 40.0%
CTGTCGACCATAATAATACG+TGG - chr4.3:81952222-81952241 None:intergenic 40.0%
GAAGAAGTCACACACAACAT+TGG + chr4.3:81951231-81951250 MS.gene037551:CDS 40.0%
GAAGTGAAGGTGATAAGCAT+TGG + chr4.3:81952476-81952495 MS.gene037551:CDS 40.0%
GAGGGTTCATGGTTTAACAA+TGG - chr4.3:81951313-81951332 None:intergenic 40.0%
GTTGAGTTCCTTAGAGATGT+TGG + chr4.3:81951176-81951195 MS.gene037551:CDS 40.0%
TAGAACTATGCTTCACCCTT+TGG + chr4.3:81951458-81951477 MS.gene037551:intron 40.0%
TCTTGACGATCTTGAAGTGA+AGG + chr4.3:81952463-81952482 MS.gene037551:CDS 40.0%
TTCACTTCAAGATCGTCAAG+AGG - chr4.3:81952464-81952483 None:intergenic 40.0%
TTGTAGGTCATTGTTGTCAG+TGG - chr4.3:81950773-81950792 None:intergenic 40.0%
TTTCTGATATCATCGGGACA+AGG - chr4.3:81951924-81951943 None:intergenic 40.0%
! ATGACTCTGAGAGTTGTTGT+AGG - chr4.3:81950789-81950808 None:intergenic 40.0%
! CATCTCTAAGGAACTCAACA+TGG - chr4.3:81951175-81951194 None:intergenic 40.0%
! CCATAATAATACGTGGGTTG+TGG - chr4.3:81952215-81952234 None:intergenic 40.0%
! CTGATGCAAATTGGTTAGCT+GGG - chr4.3:81951887-81951906 None:intergenic 40.0%
! GGGTCCATTGTAAAGCTTTT+AGG + chr4.3:81952050-81952069 MS.gene037551:CDS 40.0%
! TCTATTAGGAGGTTTTCCAC+AGG - chr4.3:81951040-81951059 None:intergenic 40.0%
!! CATCGTCTGAGTTGAGATTT+TGG - chr4.3:81950852-81950871 None:intergenic 40.0%
!! CTTAGAGATGTTGGTTCCAT+TGG + chr4.3:81951185-81951204 MS.gene037551:CDS 40.0%
!! GTACAAGAGTGTTCAGAACT+TGG + chr4.3:81952106-81952125 MS.gene037551:CDS 40.0%
!! TTTTGGCTAGCATTAGTGGT+GGG + chr4.3:81951726-81951745 MS.gene037551:intron 40.0%
!!! TTTTTGGCTAGCATTAGTGG+TGG + chr4.3:81951725-81951744 MS.gene037551:intron 40.0%
AAACCATGAACCCTCACTTG+AGG + chr4.3:81951318-81951337 MS.gene037551:intron 45.0%
AAACCTCAAGTGAGGGTTCA+TGG - chr4.3:81951324-81951343 None:intergenic 45.0%
AACAAATGTTGCTGCGTCCA+AGG + chr4.3:81950893-81950912 MS.gene037551:CDS 45.0%
AATAAGGAATGTTGAGGGGC+TGG - chr4.3:81952187-81952206 None:intergenic 45.0%
ACACAACATTGGCAAGCAAG+AGG + chr4.3:81951242-81951261 MS.gene037551:CDS 45.0%
ACACATCCAGCAAAAGAGTC+AGG - chr4.3:81952077-81952096 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG + chr4.3:81950724-81950743 MS.gene037551:CDS 45.0%
AGCTTCCTTACCATGCCATT+GGG + chr4.3:81952029-81952048 MS.gene037551:CDS 45.0%
AGCTTTACAATGGACCCCAA+TGG - chr4.3:81952047-81952066 None:intergenic 45.0%
AGGCGAAGATATACTTGAGG+AGG - chr4.3:81951809-81951828 None:intergenic 45.0%
CAAAACCTTGATTGCACCTG+TGG + chr4.3:81951021-81951040 MS.gene037551:CDS 45.0%
CTAAGAATTTGGATACGGGC+CGG + chr4.3:81951064-81951083 MS.gene037551:CDS 45.0%
CTGGTATAGCACCACAACTT+AGG + chr4.3:81952153-81952172 MS.gene037551:CDS 45.0%
GAGAGGCGAAGATATACTTG+AGG - chr4.3:81951812-81951831 None:intergenic 45.0%
GGAGTCTTCGTTAGAGAAAG+TGG + chr4.3:81951105-81951124 MS.gene037551:CDS 45.0%
GTCAAGTCTGCATAACCCAA+TGG - chr4.3:81951204-81951223 None:intergenic 45.0%
TAAGAATTTGGATACGGGCC+GGG + chr4.3:81951065-81951084 MS.gene037551:CDS 45.0%
TAGCTTCCTTACCATGCCAT+TGG + chr4.3:81952028-81952047 MS.gene037551:CDS 45.0%
TAGGTCATTGTTGTCAGTGG+TGG - chr4.3:81950770-81950789 None:intergenic 45.0%
TCTAACGAAGACTCCACTTG+AGG - chr4.3:81951100-81951119 None:intergenic 45.0%
TGGTACCAAGTGGCAAAGAT+AGG - chr4.3:81950732-81950751 None:intergenic 45.0%
TGTTGTCAGTGGTGGCTAAA+AGG - chr4.3:81950762-81950781 None:intergenic 45.0%
! GACTCTTTTGCTGGATGTGT+TGG + chr4.3:81952077-81952096 MS.gene037551:CDS 45.0%
! GTTTTCCACAGGTGCAATCA+AGG - chr4.3:81951029-81951048 None:intergenic 45.0%
! TGGTTTGGACACCTAAGTTG+TGG - chr4.3:81952167-81952186 None:intergenic 45.0%
! TTAGGACCTGACTCTTTTGC+TGG + chr4.3:81952068-81952087 MS.gene037551:CDS 45.0%
! TTCAAGGTCGTTGATTGCAC+TGG + chr4.3:81952316-81952335 MS.gene037551:CDS 45.0%
!! CTAAGTTGTGGTGCTATACC+AGG - chr4.3:81952155-81952174 None:intergenic 45.0%
AAGAATTTGGATACGGGCCG+GGG + chr4.3:81951066-81951085 MS.gene037551:CDS 50.0%
ATTGCACTGGACCCAAGAGA+AGG + chr4.3:81952329-81952348 MS.gene037551:CDS 50.0%
ATTGTGGATAAATGCGGCCG+TGG + chr4.3:81951364-81951383 MS.gene037551:intron 50.0%
CACTTGAGGCATAATTCCCC+CGG - chr4.3:81951086-81951105 None:intergenic 50.0%
CGGCCCGTATCCAAATTCTT+AGG - chr4.3:81951066-81951085 None:intergenic 50.0%
GCTTCCTTACCATGCCATTG+GGG + chr4.3:81952030-81952049 MS.gene037551:CDS 50.0%
TACAATGGACCCCAATGGCA+TGG - chr4.3:81952042-81952061 None:intergenic 50.0%
TCACATCGGCATTGCAACCA+CGG - chr4.3:81951384-81951403 None:intergenic 50.0%
! TGCGGCTGCAACTGTGTTTT+TGG + chr4.3:81951423-81951442 MS.gene037551:intron 50.0%
AATCCCACAGCGCCTTCTCT+TGG - chr4.3:81952344-81952363 None:intergenic 55.0%
AGAATTTGGATACGGGCCGG+GGG + chr4.3:81951067-81951086 MS.gene037551:CDS 55.0%
AGGCGAACGATGGTACCAAG+TGG - chr4.3:81950742-81950761 None:intergenic 55.0%
ATCCCACAGCGCCTTCTCTT+GGG - chr4.3:81952343-81952362 None:intergenic 55.0%
CGGGGGAATTATGCCTCAAG+TGG + chr4.3:81951084-81951103 MS.gene037551:CDS 55.0%
GGTGGCTAAAAGGCGAACGA+TGG - chr4.3:81950752-81950771 None:intergenic 55.0%
TGGACCCCAATGGCATGGTA+AGG - chr4.3:81952037-81952056 None:intergenic 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - chr4.3:81952182-81952201 None:intergenic 55.0%
!! GACCCAAGAGAAGGCGCTGT+GGG + chr4.3:81952338-81952357 MS.gene037551:CDS 60.0%
!! GGACCCAAGAGAAGGCGCTG+TGG + chr4.3:81952337-81952356 MS.gene037551:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 81950604 81952535 81950604 ID=MS.gene037551
chr4.3 mRNA 81950604 81952535 81950604 ID=MS.gene037551.t1;Parent=MS.gene037551
chr4.3 exon 81950604 81951263 81950604 ID=MS.gene037551.t1.exon1;Parent=MS.gene037551.t1
chr4.3 CDS 81950604 81951263 81950604 ID=cds.MS.gene037551.t1;Parent=MS.gene037551.t1
chr4.3 exon 81951798 81952535 81951798 ID=MS.gene037551.t1.exon2;Parent=MS.gene037551.t1
chr4.3 CDS 81951798 81952535 81951798 ID=cds.MS.gene037551.t1;Parent=MS.gene037551.t1
Gene Sequence

>MS.gene037551

ATGGCTTATCGACGACAACAAATATTGTCAAAAACAATTCCCCTCAAAATCTTAGTGGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGATACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCCACCACTGACAACAATGACCTACAACAACTCTCAGAGTCATCACCATTCCTTGACAACATCAAATATCTCTACCAAATTGTCCAAAATCTCAACTCAGACGATGTTTGGAACAACTCTGTGTGCAAACAAATGTTGCTGCGTCCAAGGAATCCTTGTGAAGAATTATGCATGAAGCTGTTGTTGAATGTTGATGACACGGAGACATCAAGTAAATTTTATGTGTGTGATTCTTGTTACAAGTTCACTAATTTTCAAAACCTTGATTGCACCTGTGGAAAACCTCCTAATAGACAACCTAAGAATTTGGATACGGGCCGGGGGAATTATGCCTCAAGTGGAGTCTTCGTTAGAGAAAGTGGACAAAAGTTTTTGGTGTCCGATGATTTGAAGATTTTACCAAGTTCCATGTTGAGTTCCTTAGAGATGTTGGTTCCATTGGGTTATGCAGACTTGACTGAACTAGAAGAAGTCACACACAACATTGGCAAGCAAGAGGTTAGTCCATTACATTGACTTTTTCTTACTTACATAGGATCTTAGGCCATTGTTAAACCATGAACCCTCACTTGAGGTTTCAGTTATGTTGTGAATCTTTATTGTGGATAAATGCGGCCGTGGTTGCAATGCCGATGTGAATATCTCTAAAAACATTATTGCGGCTGCAACTGTGTTTTTGGATTACAATAATTTAGAACTATGCTTCACCCTTTGGGATATAAGTCTGCTAGTAATATCAGGAACCTGCAATTATGCTAACTTATGATTTTATTTTTGACAAGCTAAAGAGAATATATTAAAAAAGGTACTTCAAAGAAAATGCATACTAAATAAGAGAAGAAACATGTTAGGATAAGAGGTATAGTTAGGGAAGTTAAGTAGTTGGAGCATGAAATTATGTGTAATTAGAATCAATATTACTTATTTTTAATAGTATTTTACCTAGGTTCCCAAAAATTTTTTTGGCTAGCATTAGTGGTGGGTTTCCTTAAACTGTTATTCTTACAATCCATGTTTTAACATTTTGTATAGATATTGAACCTCCTCAAGTATATCTTCGCCTCTCATGAGCCTATTACAAATACAATTTTGGGAAGTAGCTCAAACAACAGAAACAACCCAGCTAACCAATTTGCATCAGTAGTTAGAGTAACACCTTGTCCCGATGATATCAGAAAAATGGATGTTAAAGTAATGCAAAGTAAGTCTCAGAAAAATATAATCTATGCAGAAGCGAACGGTGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTGGGGTCCATTGTAAAGCTTTTAGGACCTGACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTTCAGAACTTGGACCCTACTTCTGTGTTACTTAATCCTGGTATAGCACCACAACTTAGGTGTCCAAACCAGCCCCTCAACATTCCTTATTTAATACCACAACCCACGTATTATTATGGTCGACAGAAAAATGATGATCACTATGACTATTATCCTACAAAAGAAGGATTGATTTCTAAGTCATATGAACTAATTTTAAGTTCAAGGTCGTTGATTGCACTGGACCCAAGAGAAGGCGCTGTGGGATTTGTGAAGAGAGCAACATTATATGGTGTAGGAGATGATCTAAAAGCAAAACCAGTTTCTGCTAAGTTTTGCCTTTCATATCTGAAAGAACAGAATCTTCCTCTTGACGATCTTGAAGTGAAGGTGATAAGCATTGGTGAATTTGAGGTAAAGGTTGCTCTTATATTAGACTAA
Protein sequence

>MS.gene037551.t1

MAYRRQQILSKTIPLKILVDKQSNKVVFVEATKDFVDTLFSFLSLPLGTIVRLLATTDNNDLQQLSESSPFLDNIKYLYQIVQNLNSDDVWNNSVCKQMLLRPRNPCEELCMKLLLNVDDTETSSKFYVCDSCYKFTNFQNLDCTCGKPPNRQPKNLDTGRGNYASSGVFVRESGQKFLVSDDLKILPSSMLSSLEMLVPLGYADLTELEEVTHNIGKQEILNLLKYIFASHEPITNTILGSSSNNRNNPANQFASVVRVTPCPDDIRKMDVKVMQSKSQKNIIYAEANGDFVDFIFSFLTMPLGSIVKLLGPDSFAGCVGNLYKSVQNLDPTSVLLNPGIAPQLRCPNQPLNIPYLIPQPTYYYGRQKNDDHYDYYPTKEGLISKSYELILSSRSLIALDPREGAVGFVKRATLYGVGDDLKAKPVSAKFCLSYLKEQNLPLDDLEVKVISIGEFEVKVALILD