AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50817

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50817.t1 MTR_4g017410 66.795 521 132 7 8 505 4 506 0.0 646
MS.gene50817.t1 MTR_4g017440 65.476 504 156 8 4 489 5 508 0.0 597
MS.gene50817.t1 MTR_4g017830 60.000 525 175 12 3 505 2 513 0.0 566
MS.gene50817.t1 MTR_4g017610 60.816 490 170 6 1 482 1 476 0.0 563
MS.gene50817.t1 MTR_4g017550 63.080 474 149 10 27 481 3 469 0.0 546
MS.gene50817.t1 MTR_4g017470 60.577 520 152 13 1 500 1 487 0.0 530
MS.gene50817.t1 MTR_4g019340 58.097 494 150 10 12 475 37 503 3.88e-177 508
MS.gene50817.t1 MTR_4g019310 37.406 532 265 17 1 499 1 497 8.70e-95 296
MS.gene50817.t1 MTR_7g066350 34.800 500 252 12 7 485 3 449 1.01e-77 251
MS.gene50817.t1 MTR_7g066340 31.928 498 270 10 3 481 2 449 2.05e-70 232
MS.gene50817.t1 MTR_7g058710 31.631 509 284 15 1 481 1 473 7.47e-69 228
MS.gene50817.t1 MTR_7g066570 34.971 509 270 19 1 481 1 476 1.01e-67 228
MS.gene50817.t1 MTR_7g066260 33.595 509 247 16 6 481 8 458 3.44e-67 224
MS.gene50817.t1 MTR_7g012680 33.464 511 264 17 2 497 3 452 5.94e-63 212
MS.gene50817.t1 MTR_7g066430 32.370 519 292 16 14 498 5 498 1.32e-62 213
MS.gene50817.t1 MTR_7g066430 24.797 246 154 4 7 236 251 481 1.04e-13 73.9
MS.gene50817.t1 MTR_1g041315 32.394 497 245 15 9 469 10 451 1.56e-61 209
MS.gene50817.t1 MTR_7g066400 31.537 501 254 14 9 488 8 440 5.60e-61 207
MS.gene50817.t1 MTR_7g063660 31.984 494 276 16 14 482 10 468 4.73e-60 205
MS.gene50817.t1 MTR_7g063900 31.601 481 271 15 12 463 9 460 8.65e-58 199
MS.gene50817.t1 MTR_7g066580 32.165 485 247 13 19 473 1 433 9.19e-58 198
MS.gene50817.t1 MTR_7g066410 32.432 518 280 17 14 481 11 508 7.36e-57 197
MS.gene50817.t1 MTR_7g066360 34.286 490 257 15 6 481 7 445 9.95e-56 194
MS.gene50817.t1 MTR_7g066290 37.389 337 180 8 6 327 8 328 2.65e-53 186
MS.gene50817.t1 MTR_7g063880 30.739 501 286 15 1 482 1 459 1.33e-51 182
MS.gene50817.t1 MTR_7g062300 30.178 507 292 15 1 482 1 470 1.23e-50 180
MS.gene50817.t1 MTR_7g063840 28.916 498 297 15 6 482 3 464 1.55e-50 179
MS.gene50817.t1 MTR_7g062280 30.930 527 274 20 1 482 1 482 1.59e-50 180
MS.gene50817.t1 MTR_7g066480 30.208 480 246 13 14 482 17 418 3.45e-48 172
MS.gene50817.t1 MTR_7g066310 30.247 486 207 15 14 481 8 379 5.30e-48 171
MS.gene50817.t1 MTR_7g063890 30.204 490 281 15 2 459 12 472 1.04e-47 172
MS.gene50817.t1 MTR_7g063830 30.677 502 261 17 1 482 1 435 2.53e-46 167
MS.gene50817.t1 MTR_7g063920 29.376 497 266 13 1 482 1 427 2.54e-46 167
MS.gene50817.t1 MTR_7g063820 30.920 511 286 16 1 481 1 474 6.34e-46 167
MS.gene50817.t1 MTR_2g029400 28.842 475 247 12 9 473 10 403 1.12e-42 157
MS.gene50817.t1 MTR_4g019240 60.800 125 45 2 1 125 1 121 1.60e-42 150
MS.gene50817.t1 MTR_4g019240 65.079 63 14 2 443 505 112 166 5.36e-13 67.8
MS.gene50817.t1 MTR_7g058700 27.778 486 223 16 25 481 531 917 7.36e-42 160
MS.gene50817.t1 MTR_7g066490 29.167 480 237 14 14 482 17 404 1.64e-41 154
MS.gene50817.t1 MTR_7g063670 28.425 489 280 15 10 481 12 447 1.39e-38 146
MS.gene50817.t1 MTR_3g007740 27.642 492 281 14 7 481 5 438 2.74e-35 137
MS.gene50817.t1 MTR_3g065200 26.061 495 284 14 14 489 4 435 1.03e-34 135
MS.gene50817.t1 MTR_7g066280 30.435 414 226 13 102 481 1 386 5.85e-34 133
MS.gene50817.t1 MTR_1g014060 33.475 236 128 10 22 243 9 229 1.86e-26 111
MS.gene50817.t1 MTR_7g066680 31.179 263 155 6 217 465 37 287 1.33e-24 105
MS.gene50817.t1 MTR_7g062270 36.022 186 98 8 8 185 5 177 7.44e-20 92.0
MS.gene50817.t1 MTR_7g100420 29.464 336 188 15 165 481 88 393 1.05e-16 82.4
MS.gene50817.t1 MTR_7g100420 46.032 63 34 0 13 75 39 101 1.64e-11 66.6
MS.gene50817.t1 MTR_1g115440 26.939 245 138 8 14 237 12 236 2.77e-16 79.0
MS.gene50817.t1 MTR_1g115440 27.586 232 141 6 266 479 11 233 8.05e-15 74.3
MS.gene50817.t1 MTR_8g445860 26.667 255 149 6 1 237 1 235 5.59e-16 77.8
MS.gene50817.t1 MTR_8g445860 28.326 233 137 7 265 479 12 232 8.33e-16 77.4
MS.gene50817.t1 MTR_0150s0010 26.829 246 135 5 261 479 5 232 1.69e-15 76.6
MS.gene50817.t1 MTR_0150s0010 26.829 246 137 6 14 237 11 235 3.48e-14 72.8
MS.gene50817.t1 MTR_6g006800 70.833 48 12 1 1 48 1 46 2.21e-14 68.6
MS.gene50817.t1 MTR_1g115455 26.556 241 140 7 14 237 10 230 5.19e-13 68.9
MS.gene50817.t1 MTR_1g115455 26.582 237 149 8 258 479 1 227 6.51e-12 65.9
MS.gene50817.t1 MTR_8g445920 40.299 67 40 0 261 327 5 71 1.06e-11 62.0
MS.gene50817.t1 MTR_8g044890 40.299 67 40 0 261 327 5 71 1.06e-11 62.0
MS.gene50817.t1 MTR_1g115450 40.000 80 40 1 265 336 6 85 2.41e-11 62.0
MS.gene50817.t1 MTR_0150s0030 34.091 88 49 1 261 339 3 90 7.12e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50817.t1 AT5G01130 31.224 490 271 16 6 481 3 440 1.30e-58 201
MS.gene50817.t1 AT5G01150 29.240 513 299 11 7 488 3 482 4.98e-54 189
MS.gene50817.t1 AT5G01120 28.773 497 312 14 10 481 6 485 5.43e-51 182
MS.gene50817.t1 AT3G09110 34.347 329 189 8 10 327 6 318 3.33e-49 172
MS.gene50817.t1 AT5G43240 28.210 514 289 15 14 481 10 489 7.47e-48 173
MS.gene50817.t1 AT5G43240 28.210 514 289 15 14 481 10 489 7.47e-48 173
MS.gene50817.t1 AT5G43240 28.210 514 289 15 14 481 10 489 7.47e-48 173
MS.gene50817.t1 AT5G01140 30.040 506 273 16 16 499 12 458 2.36e-46 168
MS.gene50817.t1 AT3G09140 27.154 534 297 17 7 488 3 496 5.34e-46 168
MS.gene50817.t1 AT3G09140 27.049 244 154 6 258 478 1 243 2.44e-12 69.7
MS.gene50817.t1 AT3G09120 32.110 327 190 10 10 320 3 313 2.15e-41 151
MS.gene50817.t1 AT3G09140 26.335 562 294 19 7 488 3 524 6.43e-41 154
MS.gene50817.t1 AT5G37320 27.539 512 308 18 8 488 3 482 8.93e-38 145
MS.gene50817.t1 AT5G43240 26.149 348 205 8 163 481 21 345 1.56e-22 99.8

Find 105 sgRNAs with CRISPR-Local

Find 141 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAACAGCCAGCTTATTATTT+TGG 0.124078 4.1:+80042359 MS.gene50817:CDS
TCCTCCCTTTGTATAATAAT+TGG 0.173244 4.1:-80042420 None:intergenic
GTTTGTCCACTAACACTTTC+AGG 0.174169 4.1:-80041162 None:intergenic
TGGCATACAGCCATGGAATT+TGG 0.179175 4.1:+80041576 MS.gene50817:CDS
AGGTGGGTTGTCTTTGTTCT+TGG 0.216712 4.1:-80042018 None:intergenic
CGGGTCCATTGTAAAGCTTT+TGG 0.265521 4.1:+80042199 MS.gene50817:CDS
TAGTGTGGTTGAAACGTTTC+TGG 0.271192 4.1:+80041624 MS.gene50817:CDS
CACCAATAACAGAAGTAAAA+TGG 0.276087 4.1:+80042076 MS.gene50817:CDS
GTCAAATTTGATTCTTGTTT+AGG 0.299305 4.1:-80042867 None:intergenic
AGTAAGTCACATGGATTTCT+TGG 0.302049 4.1:-80041433 None:intergenic
TTGCAGCTGTTGAAGGAATT+CGG 0.312659 4.1:+80041691 MS.gene50817:CDS
TTGATCATTGTTGCAGTTGA+TGG 0.313976 4.1:-80041287 None:intergenic
AAGTTACGTGAATCAATTAT+AGG 0.328775 4.1:+80042452 MS.gene50817:CDS
AGCTTTCTTACCATGCCATT+CGG 0.346241 4.1:+80042179 MS.gene50817:CDS
ACTGGCTAGTGGACTGGGTT+TGG 0.352248 4.1:+80042809 MS.gene50817:CDS
ACCTCAATTCAGACGATGTT+TGG 0.367095 4.1:+80041377 MS.gene50817:CDS
TGCAGTTGATGGTGGCTAAA+AGG 0.368848 4.1:-80041276 None:intergenic
GCCTCTCACAAATACAATCT+TGG 0.374861 4.1:+80041986 MS.gene50817:CDS
TGCAGCTGTTGAAGGAATTC+GGG 0.386713 4.1:+80041692 MS.gene50817:CDS
TCTTGATCCCTCTTTCGCTT+TGG 0.389102 4.1:-80042392 None:intergenic
CAGAAGTAAAATGGATGTTA+AGG 0.394664 4.1:+80042085 MS.gene50817:CDS
GCTTTCTTACCATGCCATTC+GGG 0.404087 4.1:+80042180 MS.gene50817:CDS
TCTAGTGCCATCAAGGATTT+CGG 0.406512 4.1:-80042476 None:intergenic
AAATTCCATGGCTGTATGCC+AGG 0.407093 4.1:-80041574 None:intergenic
GAGAGGCTCGTTAGTGGTTA+AGG 0.413109 4.1:-80041970 None:intergenic
CATCGTCTGAATTGAGGTCT+TGG 0.415737 4.1:-80041372 None:intergenic
GACATACTTGCAGCTGTTGA+AGG 0.421440 4.1:+80041684 MS.gene50817:CDS
GGAATGTTGAAGGGTTGGTT+TGG 0.427066 4.1:-80042329 None:intergenic
ATTTACACTGGCTAGTGGAC+TGG 0.431451 4.1:+80042803 MS.gene50817:CDS
CCTCTCACAAATACAATCTT+GGG 0.454373 4.1:+80041987 MS.gene50817:CDS
TTGATGTCAAGAATGCTTCA+AGG 0.460218 4.1:-80042779 None:intergenic
GATTCCCCTGAAAGTGTTAG+TGG 0.461151 4.1:+80041156 MS.gene50817:CDS
ACACATCCAGCAAAAGAGTT+AGG 0.466364 4.1:-80042224 None:intergenic
TATAGGTCCGAAATCCTTGA+TGG 0.468124 4.1:+80042469 MS.gene50817:CDS
GGATTGAGTAACACAGAAAC+GGG 0.473027 4.1:-80042281 None:intergenic
CACGAGGAATGTTGAAGGGT+TGG 0.473876 4.1:-80042334 None:intergenic
TTTGTCCACTAACACTTTCA+GGG 0.476352 4.1:-80041161 None:intergenic
GTTTCTGTGTTACTCAATCC+TGG 0.478867 4.1:+80042284 MS.gene50817:CDS
TGGTTAAGGTATACTTGAGT+AGG 0.482160 4.1:-80041956 None:intergenic
GTACAATAGTGTGAAGAACT+TGG 0.482756 4.1:+80042256 MS.gene50817:CDS
GTTTGGAAAACCAACCTCTA+GGG 0.484257 4.1:-80042312 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4.1:-80041246 None:intergenic
GTTTCGAAAAGTAGTAAACT+TGG 0.491017 4.1:-80041527 None:intergenic
AGCTTCCTATCTCTTCCACT+TGG 0.491188 4.1:+80041241 MS.gene50817:CDS
CGAAACCTTAACTGCACTTG+TGG 0.496385 4.1:+80041544 MS.gene50817:CDS
TTCAGACTTGACTCAAATAG+AGG 0.500328 4.1:+80041717 MS.gene50817:CDS
GGTTTGGAAAACCAACCTCT+AGG 0.502092 4.1:-80042313 None:intergenic
ACTGTTGGTGCAGATTGCTT+AGG 0.504136 4.1:-80042038 None:intergenic
GTTGGTGCAGATTGCTTAGG+TGG 0.508685 4.1:-80042035 None:intergenic
AGGATTGAGTAACACAGAAA+CGG 0.515205 4.1:-80042282 None:intergenic
GACAGGTCAAAAGGTGTTGT+AGG 0.515602 4.1:+80042512 MS.gene50817:CDS
TTTACACTGGCTAGTGGACT+GGG 0.516391 4.1:+80042804 MS.gene50817:CDS
GTCTGAATTGAGGTCTTGGA+TGG 0.519900 4.1:-80041368 None:intergenic
TTCTACACGAGGAATGTTGA+AGG 0.521839 4.1:-80042339 None:intergenic
ATTTGTGAGAGGCTCGTTAG+TGG 0.522997 4.1:-80041976 None:intergenic
TTACTTCTGTTATTGGTGCA+AGG 0.527701 4.1:-80042071 None:intergenic
GCCATGGAATTTGGATTCTG+AGG 0.528284 4.1:+80041585 MS.gene50817:CDS
CCTAAGCCAAAATAATAAGC+TGG 0.530209 4.1:-80042365 None:intergenic
AAGTCCAATTATTATACAAA+GGG 0.533133 4.1:+80042416 MS.gene50817:CDS
CTTGACATCAAAATTTACAC+TGG 0.536531 4.1:+80042791 MS.gene50817:CDS
CCAACCTCTAGGGGTATACC+AGG 0.537768 4.1:-80042302 None:intergenic
TCTTGGGTCTAGTGCCATCA+AGG 0.537782 4.1:-80042483 None:intergenic
TAGGAACGCCAAAGCGAAAG+AGG 0.542595 4.1:+80042384 MS.gene50817:CDS
CCCTCAGAATCCAAATTCCA+TGG 0.542858 4.1:-80041586 None:intergenic
TCCAATTATTATACAAAGGG+AGG 0.543047 4.1:+80042419 MS.gene50817:CDS
TCAAAATTTACACTGGCTAG+TGG 0.545427 4.1:+80042798 MS.gene50817:CDS
ATCATTGTTGCAGTTGATGG+TGG 0.547051 4.1:-80041284 None:intergenic
ACCAAACATCGTCTGAATTG+AGG 0.548115 4.1:-80041378 None:intergenic
CTTCAAGATCATCAAGAGGA+AGG 0.549012 4.1:-80042631 None:intergenic
CAAGTCCAATTATTATACAA+AGG 0.557972 4.1:+80042415 MS.gene50817:CDS
AGACGCGAATAAACAGAAAG+TGG 0.569116 4.1:-80042587 None:intergenic
AATTCCATGGCTGTATGCCA+GGG 0.570531 4.1:-80041573 None:intergenic
CCATGGAATTTGGATTCTGA+GGG 0.571821 4.1:+80041586 MS.gene50817:CDS
CCCAAGATTGTATTTGTGAG+AGG 0.572271 4.1:-80041987 None:intergenic
CAAGGCATTACTCCAACTGT+TGG 0.575256 4.1:-80042053 None:intergenic
GAAGTCAACAAAATCTCCGT+TGG 0.575489 4.1:-80042150 None:intergenic
AAGCAATCTGCACCAACAGT+TGG 0.576657 4.1:+80042041 MS.gene50817:CDS
TTGGTGCAGATTGCTTAGGT+GGG 0.579593 4.1:-80042034 None:intergenic
CCTGGTATACCCCTAGAGGT+TGG 0.584272 4.1:+80042302 MS.gene50817:CDS
TTGTCCACTAACACTTTCAG+GGG 0.591923 4.1:-80041160 None:intergenic
TTGTTCATGTTGAGAAGCCA+TGG 0.594375 4.1:-80041116 None:intergenic
GAGGAAATCACACACAACAT+TGG 0.597623 4.1:+80041736 MS.gene50817:CDS
AGGTCCCAAAAGCTTTACAA+TGG 0.609484 4.1:-80042204 None:intergenic
TACAATGGACCCGAATGGCA+TGG 0.610188 4.1:-80042189 None:intergenic
ATGATCTTTGTAGAAGCCAA+CGG 0.617543 4.1:+80042134 MS.gene50817:CDS
AAGTGTTAGTGGACAAACAA+AGG 0.621379 4.1:+80041167 MS.gene50817:CDS
TTCACTTCAAGATCATCAAG+AGG 0.622399 4.1:-80042635 None:intergenic
TTTGGAAAACCAACCTCTAG+GGG 0.622428 4.1:-80042311 None:intergenic
GATAAGCATTGGTGAAGCTG+AGG 0.623006 4.1:+80042661 MS.gene50817:CDS
GGTCTTGGATGGTTTCGTAG+AGG 0.631330 4.1:-80041357 None:intergenic
AGGAACGCCAAAGCGAAAGA+GGG 0.635468 4.1:+80042385 MS.gene50817:CDS
ACACAACATTGGCAAGCAAG+AGG 0.636713 4.1:+80041747 MS.gene50817:CDS
AAGTATGTCTCATACGAACA+AGG 0.645228 4.1:-80041670 None:intergenic
TCTACACGAGGAATGTTGAA+GGG 0.646068 4.1:-80042338 None:intergenic
AGCTTTACAATGGACCCGAA+TGG 0.646881 4.1:-80042194 None:intergenic
ATTCCATGGCTGTATGCCAG+GGG 0.663395 4.1:-80041572 None:intergenic
CAATCCTGGTATACCCCTAG+AGG 0.663504 4.1:+80042298 MS.gene50817:CDS
GGTGGCTAAAAGGCGGACAA+TGG 0.668124 4.1:-80041266 None:intergenic
GAAGTGAAGATGATAAGCAT+TGG 0.669635 4.1:+80042650 MS.gene50817:CDS
TTCATGCACAGTAAGTCACA+TGG 0.670403 4.1:-80041442 None:intergenic
AGTTGATGGTGGCTAAAAGG+CGG 0.682328 4.1:-80041273 None:intergenic
TTCCATGGCTGTATGCCAGG+GGG 0.683468 4.1:-80041571 None:intergenic
AGGCGGACAATGGTGCCAAG+TGG 0.686421 4.1:-80041256 None:intergenic
TCAGTTGAACAAGACTAGTG+TGG 0.692973 4.1:+80041609 MS.gene50817:CDS
TAAGCTGGCTGTTCTACACG+AGG 0.728528 4.1:-80042350 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAGTCCAATTATTATACAAA+GGG + chr4.1:80042416-80042435 MS.gene50817:CDS 20.0%
!! CAGAAAACAATTTATAAACT+TGG - chr4.1:80042709-80042728 None:intergenic 20.0%
!! CATTAAGAATTGAAATTGAT+TGG - chr4.1:80041866-80041885 None:intergenic 20.0%
! AAGTTACGTGAATCAATTAT+AGG + chr4.1:80042452-80042471 MS.gene50817:CDS 25.0%
! CAAGTCCAATTATTATACAA+AGG + chr4.1:80042415-80042434 MS.gene50817:CDS 25.0%
! TCAATATCTATACATCAGAA+AGG - chr4.1:80041935-80041954 None:intergenic 25.0%
!! ATCCATTTTACTTCTGTTAT+TGG - chr4.1:80042081-80042100 None:intergenic 25.0%
!! GTCAAATTTGATTCTTGTTT+AGG - chr4.1:80042870-80042889 None:intergenic 25.0%
!!! AAAAATGGATTATATGCTGT+TGG + chr4.1:80042545-80042564 MS.gene50817:CDS 25.0%
!!! TTTCTGTTATTTTAACCTCT+TGG + chr4.1:80042723-80042742 MS.gene50817:intron 25.0%
!!! TTTTTGATGTTTTCAAGGAA+TGG - chr4.1:80041337-80041356 None:intergenic 25.0%
AGAATTGAAATTGATTGGAC+AGG - chr4.1:80041861-80041880 None:intergenic 30.0%
CACCAATAACAGAAGTAAAA+TGG + chr4.1:80042076-80042095 MS.gene50817:CDS 30.0%
CAGAAGTAAAATGGATGTTA+AGG + chr4.1:80042085-80042104 MS.gene50817:CDS 30.0%
CTTGACATCAAAATTTACAC+TGG + chr4.1:80042791-80042810 MS.gene50817:CDS 30.0%
GTAAAAAAATGTGACGACAA+AGG - chr4.1:80041791-80041810 None:intergenic 30.0%
GTTTCGAAAAGTAGTAAACT+TGG - chr4.1:80041530-80041549 None:intergenic 30.0%
TCCAATTATTATACAAAGGG+AGG + chr4.1:80042419-80042438 MS.gene50817:CDS 30.0%
TCCTCCCTTTGTATAATAAT+TGG - chr4.1:80042423-80042442 None:intergenic 30.0%
TTGGACAGGTTAAAACAATA+AGG - chr4.1:80041847-80041866 None:intergenic 30.0%
! AGAGGTTTTTGATGTTTTCA+AGG - chr4.1:80041342-80041361 None:intergenic 30.0%
!!! AAGGAACAAAGTTGTTTTTG+TGG + chr4.1:80041186-80041205 MS.gene50817:CDS 30.0%
AAAGCCTACAACACAAGTAA+AGG - chr4.1:80042753-80042772 None:intergenic 35.0%
AAGCCTACAACACAAGTAAA+GGG - chr4.1:80042752-80042771 None:intergenic 35.0%
AAGTATGTCTCATACGAACA+AGG - chr4.1:80041673-80041692 None:intergenic 35.0%
AGGATTGAGTAACACAGAAA+CGG - chr4.1:80042285-80042304 None:intergenic 35.0%
AGTAAGTCACATGGATTTCT+TGG - chr4.1:80041436-80041455 None:intergenic 35.0%
ATGATCTTTGTAGAAGCCAA+CGG + chr4.1:80042134-80042153 MS.gene50817:CDS 35.0%
ATTCCCTTTACTTGTGTTGT+AGG + chr4.1:80042746-80042765 MS.gene50817:intron 35.0%
CCTAAGCCAAAATAATAAGC+TGG - chr4.1:80042368-80042387 None:intergenic 35.0%
CCTCTCACAAATACAATCTT+GGG + chr4.1:80041987-80042006 MS.gene50817:CDS 35.0%
GAAGTGAAGATGATAAGCAT+TGG + chr4.1:80042650-80042669 MS.gene50817:CDS 35.0%
GTACAATAGTGTGAAGAACT+TGG + chr4.1:80042256-80042275 MS.gene50817:CDS 35.0%
GTAGGATTTGTGCAGAAAAA+TGG + chr4.1:80042530-80042549 MS.gene50817:CDS 35.0%
GTGAAGCTTCATGATTAAGA+CGG - chr4.1:80041825-80041844 None:intergenic 35.0%
TGAAGCTTCATGATTAAGAC+GGG - chr4.1:80041824-80041843 None:intergenic 35.0%
TGGTTAAGGTATACTTGAGT+AGG - chr4.1:80041959-80041978 None:intergenic 35.0%
TTACTTCTGTTATTGGTGCA+AGG - chr4.1:80042074-80042093 None:intergenic 35.0%
TTCACTTCAAGATCATCAAG+AGG - chr4.1:80042638-80042657 None:intergenic 35.0%
TTCAGACTTGACTCAAATAG+AGG + chr4.1:80041717-80041736 MS.gene50817:CDS 35.0%
TTGATCATTGTTGCAGTTGA+TGG - chr4.1:80041290-80041309 None:intergenic 35.0%
TTGATGTCAAGAATGCTTCA+AGG - chr4.1:80042782-80042801 None:intergenic 35.0%
TTTGTCCACTAACACTTTCA+GGG - chr4.1:80041164-80041183 None:intergenic 35.0%
! AAGTGTTAGTGGACAAACAA+AGG + chr4.1:80041167-80041186 MS.gene50817:CDS 35.0%
! GAACAGCCAGCTTATTATTT+TGG + chr4.1:80042359-80042378 MS.gene50817:CDS 35.0%
! TCAAAATTTACACTGGCTAG+TGG + chr4.1:80042798-80042817 MS.gene50817:CDS 35.0%
!!! CCAGCTTATTATTTTGGCTT+AGG + chr4.1:80042365-80042384 MS.gene50817:CDS 35.0%
AAAATGTGACGACAAAGGAG+AGG - chr4.1:80041786-80041805 None:intergenic 40.0%
AAATGTGACGACAAAGGAGA+GGG - chr4.1:80041785-80041804 None:intergenic 40.0%
ACAAGTAAAGGGAATCCAAG+AGG - chr4.1:80042741-80042760 None:intergenic 40.0%
ACACATCCAGCAAAAGAGTT+AGG - chr4.1:80042227-80042246 None:intergenic 40.0%
ACCAAACATCGTCTGAATTG+AGG - chr4.1:80041381-80041400 None:intergenic 40.0%
ACCTCAATTCAGACGATGTT+TGG + chr4.1:80041377-80041396 MS.gene50817:CDS 40.0%
AGACGCGAATAAACAGAAAG+TGG - chr4.1:80042590-80042609 None:intergenic 40.0%
AGCTTTCTTACCATGCCATT+CGG + chr4.1:80042179-80042198 MS.gene50817:CDS 40.0%
AGGTCCCAAAAGCTTTACAA+TGG - chr4.1:80042207-80042226 None:intergenic 40.0%
CCATGGAATTTGGATTCTGA+GGG + chr4.1:80041586-80041605 MS.gene50817:CDS 40.0%
CCCAAGATTGTATTTGTGAG+AGG - chr4.1:80041990-80042009 None:intergenic 40.0%
CTTCAAGATCATCAAGAGGA+AGG - chr4.1:80042634-80042653 None:intergenic 40.0%
GAAGTCAACAAAATCTCCGT+TGG - chr4.1:80042153-80042172 None:intergenic 40.0%
GAGGAAATCACACACAACAT+TGG + chr4.1:80041736-80041755 MS.gene50817:CDS 40.0%
GCCTCTCACAAATACAATCT+TGG + chr4.1:80041986-80042005 MS.gene50817:CDS 40.0%
GGATTGAGTAACACAGAAAC+GGG - chr4.1:80042284-80042303 None:intergenic 40.0%
GTTTGGAAAACCAACCTCTA+GGG - chr4.1:80042315-80042334 None:intergenic 40.0%
GTTTGTCCACTAACACTTTC+AGG - chr4.1:80041165-80041184 None:intergenic 40.0%
TAGTGTGGTTGAAACGTTTC+TGG + chr4.1:80041624-80041643 MS.gene50817:CDS 40.0%
TATAGGTCCGAAATCCTTGA+TGG + chr4.1:80042469-80042488 MS.gene50817:CDS 40.0%
TCAGTTGAACAAGACTAGTG+TGG + chr4.1:80041609-80041628 MS.gene50817:CDS 40.0%
TCTACACGAGGAATGTTGAA+GGG - chr4.1:80042341-80042360 None:intergenic 40.0%
TCTAGTGCCATCAAGGATTT+CGG - chr4.1:80042479-80042498 None:intergenic 40.0%
TTCATGCACAGTAAGTCACA+TGG - chr4.1:80041445-80041464 None:intergenic 40.0%
TTCTACACGAGGAATGTTGA+AGG - chr4.1:80042342-80042361 None:intergenic 40.0%
TTGCAGCTGTTGAAGGAATT+CGG + chr4.1:80041691-80041710 MS.gene50817:CDS 40.0%
TTGTCCACTAACACTTTCAG+GGG - chr4.1:80041163-80041182 None:intergenic 40.0%
TTTGGAAAACCAACCTCTAG+GGG - chr4.1:80042314-80042333 None:intergenic 40.0%
! AACTCTTTTGCTGGATGTGT+TGG + chr4.1:80042227-80042246 MS.gene50817:CDS 40.0%
! GGGTCCATTGTAAAGCTTTT+GGG + chr4.1:80042200-80042219 MS.gene50817:CDS 40.0%
! GTTTCTGTGTTACTCAATCC+TGG + chr4.1:80042284-80042303 MS.gene50817:CDS 40.0%
! GTTTTCCACAAGTGCAGTTA+AGG - chr4.1:80041552-80041571 None:intergenic 40.0%
! TACAACACCTTTTGACCTGT+CGG - chr4.1:80042513-80042532 None:intergenic 40.0%
!! ATCATTGTTGCAGTTGATGG+TGG - chr4.1:80041287-80041306 None:intergenic 40.0%
AAATTCCATGGCTGTATGCC+AGG - chr4.1:80041577-80041596 None:intergenic 45.0%
AAGCAATCTGCACCAACAGT+TGG + chr4.1:80042041-80042060 MS.gene50817:CDS 45.0%
AATTCCATGGCTGTATGCCA+GGG - chr4.1:80041576-80041595 None:intergenic 45.0%
ACACAACATTGGCAAGCAAG+AGG + chr4.1:80041747-80041766 MS.gene50817:CDS 45.0%
ACTGTTGGTGCAGATTGCTT+AGG - chr4.1:80042041-80042060 None:intergenic 45.0%
AGCTTCCTATCTCTTCCACT+TGG + chr4.1:80041241-80041260 MS.gene50817:CDS 45.0%
AGCTTTACAATGGACCCGAA+TGG - chr4.1:80042197-80042216 None:intergenic 45.0%
ATTTGTGAGAGGCTCGTTAG+TGG - chr4.1:80041979-80041998 None:intergenic 45.0%
CATCGTCTGAATTGAGGTCT+TGG - chr4.1:80041375-80041394 None:intergenic 45.0%
CCCTCAGAATCCAAATTCCA+TGG - chr4.1:80041589-80041608 None:intergenic 45.0%
CGAAACCTTAACTGCACTTG+TGG + chr4.1:80041544-80041563 MS.gene50817:CDS 45.0%
GACATACTTGCAGCTGTTGA+AGG + chr4.1:80041684-80041703 MS.gene50817:CDS 45.0%
GCCATGGAATTTGGATTCTG+AGG + chr4.1:80041585-80041604 MS.gene50817:CDS 45.0%
GCTTTCTTACCATGCCATTC+GGG + chr4.1:80042180-80042199 MS.gene50817:CDS 45.0%
GGTTTGGAAAACCAACCTCT+AGG - chr4.1:80042316-80042335 None:intergenic 45.0%
GTCTGAATTGAGGTCTTGGA+TGG - chr4.1:80041371-80041390 None:intergenic 45.0%
TCTTGATCCCTCTTTCGCTT+TGG - chr4.1:80042395-80042414 None:intergenic 45.0%
TGCAGCTGTTGAAGGAATTC+GGG + chr4.1:80041692-80041711 MS.gene50817:CDS 45.0%
TGGCATACAGCCATGGAATT+TGG + chr4.1:80041576-80041595 MS.gene50817:CDS 45.0%
TTGGTGCAGATTGCTTAGGT+GGG - chr4.1:80042037-80042056 None:intergenic 45.0%
! ACAACACCTTTTGACCTGTC+GGG - chr4.1:80042512-80042531 None:intergenic 45.0%
! ATTTACACTGGCTAGTGGAC+TGG + chr4.1:80042803-80042822 MS.gene50817:CDS 45.0%
! CAAGGCATTACTCCAACTGT+TGG - chr4.1:80042056-80042075 None:intergenic 45.0%
! CGGGTCCATTGTAAAGCTTT+TGG + chr4.1:80042199-80042218 MS.gene50817:CDS 45.0%
! TTGGGACCTAACTCTTTTGC+TGG + chr4.1:80042218-80042237 MS.gene50817:CDS 45.0%
!! AGGTGGGTTGTCTTTGTTCT+TGG - chr4.1:80042021-80042040 None:intergenic 45.0%
!! AGTTGATGGTGGCTAAAAGG+CGG - chr4.1:80041276-80041295 None:intergenic 45.0%
!! GACAGGTCAAAAGGTGTTGT+AGG + chr4.1:80042512-80042531 MS.gene50817:CDS 45.0%
!! GATAAGCATTGGTGAAGCTG+AGG + chr4.1:80042661-80042680 MS.gene50817:CDS 45.0%
!! GATTCCCCTGAAAGTGTTAG+TGG + chr4.1:80041156-80041175 MS.gene50817:CDS 45.0%
!! GGAATGTTGAAGGGTTGGTT+TGG - chr4.1:80042332-80042351 None:intergenic 45.0%
!! TGCAGTTGATGGTGGCTAAA+AGG - chr4.1:80041279-80041298 None:intergenic 45.0%
!! TTTACACTGGCTAGTGGACT+GGG + chr4.1:80042804-80042823 MS.gene50817:CDS 45.0%
AGGAACGCCAAAGCGAAAGA+GGG + chr4.1:80042385-80042404 MS.gene50817:CDS 50.0%
ATTCCATGGCTGTATGCCAG+GGG - chr4.1:80041575-80041594 None:intergenic 50.0%
CAATCCTGGTATACCCCTAG+AGG + chr4.1:80042298-80042317 MS.gene50817:CDS 50.0%
CACGAGGAATGTTGAAGGGT+TGG - chr4.1:80042337-80042356 None:intergenic 50.0%
GAGAGGCTCGTTAGTGGTTA+AGG - chr4.1:80041973-80041992 None:intergenic 50.0%
GGTCTTGGATGGTTTCGTAG+AGG - chr4.1:80041360-80041379 None:intergenic 50.0%
GTTGGTGCAGATTGCTTAGG+TGG - chr4.1:80042038-80042057 None:intergenic 50.0%
TAAGCTGGCTGTTCTACACG+AGG - chr4.1:80042353-80042372 None:intergenic 50.0%
TACAATGGACCCGAATGGCA+TGG - chr4.1:80042192-80042211 None:intergenic 50.0%
TAGGAACGCCAAAGCGAAAG+AGG + chr4.1:80042384-80042403 MS.gene50817:CDS 50.0%
TCTTGGGTCTAGTGCCATCA+AGG - chr4.1:80042486-80042505 None:intergenic 50.0%
! AACACCTTTTGACCTGTCGG+GGG - chr4.1:80042510-80042529 None:intergenic 50.0%
! CAACACCTTTTGACCTGTCG+GGG - chr4.1:80042511-80042530 None:intergenic 50.0%
! GCATTGGTGAAGCTGAGGTA+AGG + chr4.1:80042666-80042685 MS.gene50817:intron 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG - chr4.1:80041249-80041268 None:intergenic 50.0%
AGATCCCCCGACAGGTCAAA+AGG + chr4.1:80042503-80042522 MS.gene50817:CDS 55.0%
CCTGGTATACCCCTAGAGGT+TGG + chr4.1:80042302-80042321 MS.gene50817:CDS 55.0%
GGTGGCTAAAAGGCGGACAA+TGG - chr4.1:80041269-80041288 None:intergenic 55.0%
TGCACTTGTGGAAAACCCCC+TGG + chr4.1:80041556-80041575 MS.gene50817:CDS 55.0%
TTCCATGGCTGTATGCCAGG+GGG - chr4.1:80041574-80041593 None:intergenic 55.0%
TTGACCTGTCGGGGGATCTT+GGG - chr4.1:80042502-80042521 None:intergenic 55.0%
TTTGACCTGTCGGGGGATCT+TGG - chr4.1:80042503-80042522 None:intergenic 55.0%
! CCAACCTCTAGGGGTATACC+AGG - chr4.1:80042305-80042324 None:intergenic 55.0%
!! ACTGGCTAGTGGACTGGGTT+TGG + chr4.1:80042809-80042828 MS.gene50817:CDS 55.0%
AACCCCCTGGCATACAGCCA+TGG + chr4.1:80041569-80041588 MS.gene50817:CDS 60.0%
TAGACCCAAGATCCCCCGAC+AGG + chr4.1:80042495-80042514 MS.gene50817:CDS 60.0%
! AGGCGGACAATGGTGCCAAG+TGG - chr4.1:80041259-80041278 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80041118 80042899 80041118 ID=MS.gene50817
chr4.1 mRNA 80041118 80042899 80041118 ID=MS.gene50817.t1;Parent=MS.gene50817
chr4.1 exon 80041118 80041768 80041118 ID=MS.gene50817.t1.exon1;Parent=MS.gene50817.t1
chr4.1 CDS 80041118 80041768 80041118 ID=cds.MS.gene50817.t1;Parent=MS.gene50817.t1
chr4.1 exon 80041948 80042682 80041948 ID=MS.gene50817.t1.exon2;Parent=MS.gene50817.t1
chr4.1 CDS 80041948 80042682 80041948 ID=cds.MS.gene50817.t1;Parent=MS.gene50817.t1
chr4.1 exon 80042768 80042899 80042768 ID=MS.gene50817.t1.exon3;Parent=MS.gene50817.t1
chr4.1 CDS 80042768 80042899 80042768 ID=cds.MS.gene50817.t1;Parent=MS.gene50817.t1
Gene Sequence

>MS.gene50817

ATGGCTTCTCAACATGAACAACAAGAAGAATCAAAAACGATTCCCCTGAAAGTGTTAGTGGACAAACAAAGGAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGATACTATTTTCAGCTTCCTATCTCTTCCACTTGGCACCATTGTCCGCCTTTTAGCCACCATCAACTGCAACAATGATCAACAAAAACAACTCTCAGAATCATCACCATTCCTTGAAAACATCAAAAACCTCTACGAAACCATCCAAGACCTCAATTCAGACGATGTTTGGTACAACTCTTTCTGCAAACATATGTTGTTGCATCCAAGAAATCCATGTGACTTACTGTGCATGAAGTTGTTCTTGAATATTGATGATACTGAAACATCAAATAAATTTTTTGTGTGTCATGCTTGTTCCAAGTTTACTACTTTTCGAAACCTTAACTGCACTTGTGGAAAACCCCCTGGCATACAGCCATGGAATTTGGATTCTGAGGGTCAGTTGAACAAGACTAGTGTGGTTGAAACGTTTCTGGTGTCTGATGATTTGAAGATTGTTCCTTGTTCGTATGAGACATACTTGCAGCTGTTGAAGGAATTCGGGTATTCAGACTTGACTCAAATAGAGGAAATCACACACAACATTGGCAAGCAAGAGGTAACTACTTATGCCCTCTCCTTTGTCGTCACATTTTTTTACGTTTTTTATACCCGTCTTAATCATGAAGCTTCACCTTATTGTTTTAACCTGTCCAATCAATTTCAATTCTTAATGTTGTGATTTATTGCTTCATTTTTTTTTAAGAAGAAATTGATCACTTCCTTTCTGATGTATAGATATTGAACCTACTCAAGTATACCTTAACCACTAACGAGCCTCTCACAAATACAATCTTGGGATGTAGCTCCAAGAACAAAGACAACCCACCTAAGCAATCTGCACCAACAGTTGGAGTAATGCCTTGCACCAATAACAGAAGTAAAATGGATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGATGATCTTTGTAGAAGCCAACGGAGATTTTGTTGACTTCATTTTTAGCTTTCTTACCATGCCATTCGGGTCCATTGTAAAGCTTTTGGGACCTAACTCTTTTGCTGGATGTGTTGGTAATTTGTACAATAGTGTGAAGAACTTGGATCCCGTTTCTGTGTTACTCAATCCTGGTATACCCCTAGAGGTTGGTTTTCCAAACCAACCCTTCAACATTCCTCGTGTAGAACAGCCAGCTTATTATTTTGGCTTAGGAACGCCAAAGCGAAAGAGGGATCAAGACAAGTCCAATTATTATACAAAGGGAGGAGTGATTTCTAAGTTACGTGAATCAATTATAGGTCCGAAATCCTTGATGGCACTAGACCCAAGATCCCCCGACAGGTCAAAAGGTGTTGTAGGATTTGTGCAGAAAAATGGATTATATGCTGTTGGAGATGATCTAAAAGTAAAACCACTTTCTGTTTATTCGCGTCTTTCATATTTGAAAGAGTTAAGCCTTCCTCTTGATGATCTTGAAGTGAAGATGATAAGCATTGGTGAAGCTGAGGTAAGGTTGCTATTATTTTACGACCAAGTTTATAAATTGTTTTCTGTTATTTTAACCTCTTGGATTCCCTTTACTTGTGTTGTAGGCTTTGAGATTCCTTGAAGCATTCTTGACATCAAAATTTACACTGGCTAGTGGACTGGGTTTGGATGTGCATAAAAAAGAATTCACTTCTTTGAAAGTGCCTAAACAAGAATCAAATTTGACATCACAGTAA
Protein sequence

>MS.gene50817.t1

MASQHEQQEESKTIPLKVLVDKQRNKVVFVEATKDFVDTIFSFLSLPLGTIVRLLATINCNNDQQKQLSESSPFLENIKNLYETIQDLNSDDVWYNSFCKHMLLHPRNPCDLLCMKLFLNIDDTETSNKFFVCHACSKFTTFRNLNCTCGKPPGIQPWNLDSEGQLNKTSVVETFLVSDDLKIVPCSYETYLQLLKEFGYSDLTQIEEITHNIGKQEILNLLKYTLTTNEPLTNTILGCSSKNKDNPPKQSAPTVGVMPCTNNRSKMDVKVVQSKSQKKMIFVEANGDFVDFIFSFLTMPFGSIVKLLGPNSFAGCVGNLYNSVKNLDPVSVLLNPGIPLEVGFPNQPFNIPRVEQPAYYFGLGTPKRKRDQDKSNYYTKGGVISKLRESIIGPKSLMALDPRSPDRSKGVVGFVQKNGLYAVGDDLKVKPLSVYSRLSYLKELSLPLDDLEVKMISIGEAEALRFLEAFLTSKFTLASGLGLDVHKKEFTSLKVPKQESNLTSQ