AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70044

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70044.t1 MTR_4g017410 71.067 356 46 7 1 304 1 351 8.02e-168 476
MS.gene70044.t1 MTR_4g017830 58.286 350 95 5 1 303 1 346 1.12e-133 390
MS.gene70044.t1 MTR_4g017440 60.282 355 87 6 1 303 1 353 9.20e-133 387
MS.gene70044.t1 MTR_4g017470 61.176 340 70 5 1 304 1 314 1.71e-126 370
MS.gene70044.t1 MTR_4g017550 60.125 321 84 5 26 304 3 321 3.40e-125 366
MS.gene70044.t1 MTR_4g017610 57.349 347 99 6 1 303 1 342 4.63e-124 363
MS.gene70044.t1 MTR_4g019340 57.224 353 86 8 10 303 36 382 2.93e-123 362
MS.gene70044.t1 MTR_4g019340 30.876 217 118 8 15 220 306 501 3.35e-16 79.0
MS.gene70044.t1 MTR_4g019310 35.380 342 159 9 1 293 1 329 3.50e-52 179
MS.gene70044.t1 MTR_4g019310 27.869 244 138 9 13 235 267 493 7.63e-11 63.2
MS.gene70044.t1 MTR_4g019240 62.992 127 37 4 1 123 1 121 9.18e-45 150
MS.gene70044.t1 MTR_7g066350 32.464 345 165 9 3 297 2 328 9.37e-42 150
MS.gene70044.t1 MTR_7g066340 32.456 342 156 10 2 291 6 324 1.02e-41 150
MS.gene70044.t1 MTR_7g066340 27.391 230 127 7 1 226 254 447 1.20e-15 77.4
MS.gene70044.t1 MTR_7g066260 34.783 276 144 7 13 266 16 277 1.47e-40 147
MS.gene70044.t1 MTR_7g066260 26.364 220 133 6 8 226 265 456 1.27e-12 68.6
MS.gene70044.t1 MTR_7g066570 36.644 292 132 12 1 266 1 265 4.01e-40 147
MS.gene70044.t1 MTR_7g066570 27.197 239 135 8 9 226 254 474 2.85e-13 70.5
MS.gene70044.t1 MTR_7g058710 32.990 291 140 8 1 266 1 261 1.33e-38 142
MS.gene70044.t1 MTR_7g066410 31.445 353 162 11 13 297 11 351 6.35e-38 140
MS.gene70044.t1 MTR_7g066290 34.948 289 147 9 1 266 5 275 6.49e-35 131
MS.gene70044.t1 MTR_7g066360 35.636 275 136 9 13 264 15 271 1.94e-34 130
MS.gene70044.t1 MTR_7g066430 29.714 350 174 10 13 304 5 340 2.86e-34 130
MS.gene70044.t1 MTR_1g041315 33.559 295 148 10 5 266 7 286 8.01e-34 129
MS.gene70044.t1 MTR_2g029400 33.220 295 137 10 13 297 15 259 9.12e-34 127
MS.gene70044.t1 MTR_7g012680 32.895 304 138 13 7 286 11 272 1.44e-33 128
MS.gene70044.t1 MTR_7g063880 31.322 348 174 13 1 304 1 327 2.04e-33 128
MS.gene70044.t1 MTR_7g063660 30.655 336 166 10 8 298 5 318 1.06e-32 126
MS.gene70044.t1 MTR_7g063660 26.316 228 139 4 13 225 251 464 3.21e-13 70.1
MS.gene70044.t1 MTR_7g063900 31.105 344 169 13 10 303 8 333 2.08e-32 125
MS.gene70044.t1 MTR_7g066400 30.994 342 156 13 1 297 1 307 2.87e-31 121
MS.gene70044.t1 MTR_7g066480 37.118 229 122 6 13 230 17 234 1.69e-30 119
MS.gene70044.t1 MTR_7g063820 33.728 338 168 15 1 298 1 322 1.44e-29 117
MS.gene70044.t1 MTR_7g063890 34.177 237 117 8 13 229 28 245 3.12e-29 116
MS.gene70044.t1 MTR_7g063840 34.818 247 113 9 7 229 5 227 7.19e-29 115
MS.gene70044.t1 MTR_7g063830 29.769 346 168 14 13 303 11 336 2.26e-27 110
MS.gene70044.t1 MTR_7g062300 28.571 357 182 11 1 303 1 338 8.13e-27 109
MS.gene70044.t1 MTR_1g014060 32.444 225 113 7 21 224 9 215 1.57e-25 105
MS.gene70044.t1 MTR_7g066490 34.156 243 116 10 13 240 17 230 1.19e-24 103
MS.gene70044.t1 MTR_7g066580 29.368 269 124 7 18 266 1 223 2.72e-24 102
MS.gene70044.t1 MTR_7g066580 29.583 240 129 8 11 225 214 438 8.54e-13 68.9
MS.gene70044.t1 MTR_7g062280 29.086 361 163 13 13 303 11 348 5.23e-24 102
MS.gene70044.t1 MTR_7g058700 27.507 349 156 12 10 298 509 820 2.64e-22 97.8
MS.gene70044.t1 MTR_7g063670 30.877 285 150 9 1 266 4 260 1.40e-21 94.7
MS.gene70044.t1 MTR_3g007740 28.247 308 163 8 1 284 1 274 2.55e-21 94.0
MS.gene70044.t1 MTR_7g066310 29.487 312 132 11 7 291 4 254 4.34e-21 92.8
MS.gene70044.t1 MTR_7g063920 31.034 232 121 7 1 230 1 195 4.94e-21 93.2
MS.gene70044.t1 MTR_3g065200 31.364 220 108 7 13 215 4 197 8.28e-20 89.7
MS.gene70044.t1 MTR_7g063690 31.004 229 97 8 1 228 1 169 8.21e-18 81.3
MS.gene70044.t1 MTR_1g115440 27.500 240 136 7 13 229 12 236 1.28e-17 80.9
MS.gene70044.t1 MTR_7g062270 33.679 193 96 9 1 177 1 177 3.02e-17 81.6
MS.gene70044.t1 MTR_8g445860 28.814 236 135 6 13 229 14 235 8.30e-17 78.6
MS.gene70044.t1 MTR_0150s0010 29.084 251 140 8 1 229 1 235 4.20e-16 76.6
MS.gene70044.t1 MTR_6g006800 72.340 47 12 1 1 47 1 46 7.66e-14 65.9
MS.gene70044.t1 MTR_7g100420 67.442 43 14 0 12 54 39 81 7.51e-12 65.9
MS.gene70044.t1 MTR_1g115455 38.298 94 42 3 13 105 10 88 2.23e-11 63.2
MS.gene70044.t1 MTR_1g115450 33.945 109 58 2 13 121 8 102 2.69e-11 60.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70044.t1 AT5G43240 35.547 256 132 6 13 245 10 255 5.79e-37 138
MS.gene70044.t1 AT5G43240 35.547 256 132 6 13 245 10 255 5.79e-37 138
MS.gene70044.t1 AT5G43240 35.547 256 132 6 13 245 10 255 5.79e-37 138
MS.gene70044.t1 AT5G01120 32.830 265 145 5 13 258 10 260 4.27e-35 133
MS.gene70044.t1 AT5G01130 33.977 259 136 8 13 256 10 248 1.60e-34 130
MS.gene70044.t1 AT3G09120 34.440 241 119 8 13 229 7 232 5.79e-34 126
MS.gene70044.t1 AT5G01150 35.498 231 123 6 15 229 12 232 1.41e-33 129
MS.gene70044.t1 AT3G09140 29.565 345 174 10 11 295 8 343 3.66e-33 127
MS.gene70044.t1 AT3G09110 31.307 329 164 9 9 291 6 318 3.70e-33 125
MS.gene70044.t1 AT3G09140 27.346 373 174 11 11 295 8 371 2.84e-28 114
MS.gene70044.t1 AT5G01140 28.440 327 159 10 15 297 12 307 4.90e-27 110
MS.gene70044.t1 AT5G37320 29.129 333 162 12 19 291 18 336 3.18e-23 99.8

Find 63 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATGCTTCACATGGATTTC+TGG 0.119581 4.2:+84656427 None:intergenic
CATCATTTGAATTGAGATTT+TGG 0.129092 4.2:+84656489 None:intergenic
CTCTTATAAAGACCTCATTT+TGG 0.187757 4.2:+84656276 None:intergenic
GTCATTGTTGTTGTTATTCC+TGG 0.237924 4.2:+84656568 None:intergenic
CTTGAAGCTGTTGATGGAAT+TGG 0.253662 4.2:-84656189 MS.gene70044:CDS
AGGTCGGTTGTCTTTGTTCT+TGG 0.264894 4.2:+84655217 None:intergenic
AGAAAATGGATCAATGTTTC+TGG 0.270658 4.2:-84656255 MS.gene70044:CDS
AGGGTCCATTGTAAAGCTTT+TGG 0.288911 4.2:-84655036 MS.gene70044:CDS
CACCAGTGACAACAGGAAAT+TGG 0.303337 4.2:-84655159 MS.gene70044:CDS
TATTCCTGGTGGTGGCTAAA+AGG 0.313041 4.2:+84656582 None:intergenic
ATCTCAATTCAAATGATGTT+TGG 0.325115 4.2:-84656484 MS.gene70044:CDS
GAGGTCTTTATAAGAGAAAA+TGG 0.340080 4.2:-84656269 MS.gene70044:CDS
GGAATTGGGGTATTCAGATT+TGG 0.342273 4.2:-84656174 MS.gene70044:CDS
GACTTACTTGAAGCTGTTGA+TGG 0.369037 4.2:-84656195 MS.gene70044:CDS
TTGAAGCTGTTGATGGAATT+GGG 0.369604 4.2:-84656188 MS.gene70044:CDS
AATGCTTCACATGGATTTCT+GGG 0.382524 4.2:+84656428 None:intergenic
CCCTCAGAATCCAAGTTCTT+AGG 0.386132 4.2:+84656314 None:intergenic
CAAAGGCTCATGAGAGGTTA+AGG 0.417761 4.2:+84655265 None:intergenic
TTCTTAGGCTGTCTATTAGT+GGG 0.418576 4.2:+84656329 None:intergenic
ACTGCTGATGCAAGTTGGTT+AGG 0.427676 4.2:+84655197 None:intergenic
GTTCTTAGGCTGTCTATTAG+TGG 0.429169 4.2:+84656328 None:intergenic
TTCAGATTTGGCTCAAATAG+AGG 0.433676 4.2:-84656162 MS.gene70044:CDS
AATTCCCCTCAAAATGTTAG+TGG 0.442870 4.2:-84656702 MS.gene70044:CDS
TGCCTTGCACCAGTGACAAC+AGG 0.457008 4.2:-84655166 MS.gene70044:CDS
ATGGTATTGATTCTGATTGT+TGG 0.459184 4.2:+84656546 None:intergenic
CAGAATCTTGATTGCACCTG+TGG 0.459192 4.2:-84656356 MS.gene70044:CDS
ACACACCCATCAAAAGAGTT+AGG 0.466364 4.2:+84655011 None:intergenic
TAATAGACAGCCTAAGAACT+TGG 0.472478 4.2:-84656324 MS.gene70044:CDS
AAAAGCATTTGTTTGCAGAC+AGG 0.473427 4.2:+84656455 None:intergenic
ATCCAATTTCCTGTTGTCAC+TGG 0.480035 4.2:+84655157 None:intergenic
CATGAGAGGTTAAGGTGTGC+TGG 0.483568 4.2:+84655273 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4.2:+84656612 None:intergenic
AGCTTCCTATCTCTTCCACT+TGG 0.491188 4.2:-84656617 MS.gene70044:CDS
GTTGTTGTTATTCCTGGTGG+TGG 0.496331 4.2:+84656574 None:intergenic
GTACAATAGTGTGAAGAAGT+TGG 0.515165 4.2:-84654979 MS.gene70044:CDS
GCCTAAGAACTTGGATTCTG+AGG 0.520425 4.2:-84656315 MS.gene70044:CDS
GGATCGAGTAACACAGACTT+GGG 0.525345 4.2:+84654954 None:intergenic
GAGAGTGTCAACAAAGTCCT+TGG 0.531473 4.2:+84656643 None:intergenic
CTCTAACTGCTGATGCAAGT+TGG 0.535856 4.2:+84655192 None:intergenic
AAGTAAGTCACAGAGGAACT+TGG 0.549971 4.2:+84656209 None:intergenic
AGGTTAAGGTGTGCTGGAGG+AGG 0.551038 4.2:+84655279 None:intergenic
TTTCTGATGTTGTCAAGGAA+TGG 0.556391 4.2:+84656527 None:intergenic
TTGTAGCTGTTCAGAAGCCA+TGG 0.558598 4.2:+84656739 None:intergenic
TTCCTGTTGTCACTGGTGCA+AGG 0.566131 4.2:+84655164 None:intergenic
AGGATCGAGTAACACAGACT+TGG 0.584803 4.2:+84654953 None:intergenic
AGAGGTTTCTGATGTTGTCA+AGG 0.592804 4.2:+84656522 None:intergenic
TTCATGCACAATGCTTCACA+TGG 0.593963 4.2:+84656419 None:intergenic
TGAGAGTAATGCCCAAAATG+AGG 0.598804 4.2:-84656288 MS.gene70044:CDS
CTGATGCAAGTTGGTTAGGT+CGG 0.600509 4.2:+84655201 None:intergenic
ATTGTTGTTGTTATTCCTGG+TGG 0.607529 4.2:+84656571 None:intergenic
AGGTCCCAAAAGCTTTACAA+TGG 0.609484 4.2:+84655031 None:intergenic
AAATGTTAGTGGACAAACAA+AGG 0.609948 4.2:-84656691 MS.gene70044:CDS
CCTAAGAACTTGGATTCTGA+GGG 0.620194 4.2:-84656314 MS.gene70044:CDS
GAGGAAGTCACACACAACAT+TGG 0.621785 4.2:-84656143 MS.gene70044:CDS
ACACAACATTGGCAAACAAG+AGG 0.630703 4.2:-84656132 MS.gene70044:intron
TGAAGCTGTTGATGGAATTG+GGG 0.640294 4.2:-84656187 MS.gene70044:CDS
CTTCACAGGTGTGCTATACG+AGG 0.643795 4.2:+84654933 None:intergenic
GAGAGGTTAAGGTGTGCTGG+AGG 0.655982 4.2:+84655276 None:intergenic
ATTTGTCAAAGGCTCATGAG+AGG 0.656727 4.2:+84655259 None:intergenic
AGGCGAACGATGGTGCCAAG+TGG 0.699497 4.2:+84656602 None:intergenic
GGTGGCTAAAAGGCGAACGA+TGG 0.723514 4.2:+84656592 None:intergenic
CAGCTTCAAGTAAGTCACAG+AGG 0.741452 4.2:+84656202 None:intergenic
GATCGAGTAACACAGACTTG+GGG 0.769489 4.2:+84654955 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAATAGATCTATTAAAGAA+AGG + chr4.2:84656292-84656311 None:intergenic 15.0%
!! AATTAATATTCACAATCATT+TGG - chr4.2:84655604-84655623 MS.gene70044:intron 15.0%
!! ACAAAAATAAAGAAATACTA+TGG - chr4.2:84655786-84655805 MS.gene70044:intron 15.0%
!! CAAAAATAAAGAAATACTAT+GGG - chr4.2:84655787-84655806 MS.gene70044:intron 15.0%
!! AATATCAAATAATAGTCGTA+TGG + chr4.2:84655961-84655980 None:intergenic 20.0%
!! TAATTAAAAAAATTGCATCC+TGG - chr4.2:84656041-84656060 MS.gene70044:intron 20.0%
!!! AACTTTTAGTTGAAATCTAA+AGG - chr4.2:84655865-84655884 MS.gene70044:intron 20.0%
!!! TTTTTTTGTTAGAAAACATG+TGG - chr4.2:84656204-84656223 MS.gene70044:CDS 20.0%
! ACAAGAAAAAGCAATACTAT+GGG - chr4.2:84655830-84655849 MS.gene70044:intron 25.0%
! ATCTCAATTCAAATGATGTT+TGG - chr4.2:84655188-84655207 MS.gene70044:CDS 25.0%
! CATTTACCAAACATTCTTAT+CGG + chr4.2:84655899-84655918 None:intergenic 25.0%
! GCAAAAAATGTATTTGTCAA+AGG + chr4.2:84656427-84656446 None:intergenic 25.0%
! TACAAGAAAAAGCAATACTA+TGG - chr4.2:84655829-84655848 MS.gene70044:intron 25.0%
!! ATTTGAATTGAGATTTTGGA+CGG + chr4.2:84655182-84655201 None:intergenic 25.0%
!! CATCATTTGAATTGAGATTT+TGG + chr4.2:84655186-84655205 None:intergenic 25.0%
!! TCTTATAAAGACCTCATTTT+GGG + chr4.2:84655398-84655417 None:intergenic 25.0%
!!! AGTATTGCTTTTTCTTGTAT+TGG + chr4.2:84655828-84655847 None:intergenic 25.0%
!!! GTATTGCTTTTTCTTGTATT+GGG + chr4.2:84655827-84655846 None:intergenic 25.0%
AGAAAATGGATCAATGTTTC+TGG - chr4.2:84655417-84655436 MS.gene70044:intron 30.0%
ATAATCTTTGCAGAAACCAA+TGG - chr4.2:84656571-84656590 MS.gene70044:CDS 30.0%
GAGGTCTTTATAAGAGAAAA+TGG - chr4.2:84655403-84655422 MS.gene70044:intron 30.0%
GTGAATAAGCAGTAAATTCA+AGG + chr4.2:84655731-84655750 None:intergenic 30.0%
TACTGCTTATTCACCTTAAA+GGG - chr4.2:84655737-84655756 MS.gene70044:intron 30.0%
TTACTGCTTATTCACCTTAA+AGG - chr4.2:84655736-84655755 MS.gene70044:intron 30.0%
! AAATGTTAGTGGACAAACAA+AGG - chr4.2:84654981-84655000 MS.gene70044:CDS 30.0%
! ACAAAGTATGAGAGAGTATA+AGG - chr4.2:84656008-84656027 MS.gene70044:intron 30.0%
! ATTTTTGTCAATTAGCCAAG+TGG + chr4.2:84655774-84655793 None:intergenic 30.0%
! CTCTTATAAAGACCTCATTT+TGG + chr4.2:84655399-84655418 None:intergenic 30.0%
! TTTGTCCACTAACATTTTGA+GGG + chr4.2:84654978-84654997 None:intergenic 30.0%
!! ATGGTATTGATTCTGATTGT+TGG + chr4.2:84655129-84655148 None:intergenic 30.0%
!! TAAGTACCGATAAGAATGTT+TGG - chr4.2:84655890-84655909 MS.gene70044:intron 30.0%
!!! AAGGAACAAAGTTGTTTTTG+TGG - chr4.2:84655000-84655019 MS.gene70044:CDS 30.0%
AAAGGAAATCCATATAACCC+TGG + chr4.2:84656274-84656293 None:intergenic 35.0%
AACACCACCATATTAGAGTA+AGG - chr4.2:84656150-84656169 MS.gene70044:CDS 35.0%
AATGCTTCACATGGATTTCT+GGG + chr4.2:84655247-84655266 None:intergenic 35.0%
AATTCCCCTCAAAATGTTAG+TGG - chr4.2:84654970-84654989 MS.gene70044:CDS 35.0%
CTAGTGACCTTACTCTAATA+TGG + chr4.2:84656160-84656179 None:intergenic 35.0%
GAAGTCAACAAAATCTCCAT+TGG + chr4.2:84656590-84656609 None:intergenic 35.0%
GTACAATAGTGTGAAGAAGT+TGG - chr4.2:84656693-84656712 MS.gene70044:CDS 35.0%
TAATAGACAGCCTAAGAACT+TGG - chr4.2:84655348-84655367 MS.gene70044:intron 35.0%
TAGAAAACATGTGGTTTCAG+CGG - chr4.2:84656213-84656232 MS.gene70044:CDS 35.0%
TTCTTAGGCTGTCTATTAGT+GGG + chr4.2:84655346-84655365 None:intergenic 35.0%
TTTCTGATGTTGTCAAGGAA+TGG + chr4.2:84655148-84655167 None:intergenic 35.0%
! AATTGAGATTTTGGACGGTA+CGG + chr4.2:84655177-84655196 None:intergenic 35.0%
! ATTGTTGTTGTTATTCCTGG+TGG + chr4.2:84655104-84655123 None:intergenic 35.0%
! GTCATTGTTGTTGTTATTCC+TGG + chr4.2:84655107-84655126 None:intergenic 35.0%
! GTTTGTCCACTAACATTTTG+AGG + chr4.2:84654979-84654998 None:intergenic 35.0%
! TGATCACAACTTAGTTGGTA+GGG - chr4.2:84655669-84655688 MS.gene70044:intron 35.0%
! TTCAGATTTGGCTCAAATAG+AGG - chr4.2:84655510-84655529 MS.gene70044:intron 35.0%
! TTGAAGCTGTTGATGGAATT+GGG - chr4.2:84655484-84655503 MS.gene70044:intron 35.0%
! TTGTCCACTAACATTTTGAG+GGG + chr4.2:84654977-84654996 None:intergenic 35.0%
!! AAAAGCATTTGTTTGCAGAC+AGG + chr4.2:84655220-84655239 None:intergenic 35.0%
AAAAATTGCATCCTGGCACA+AGG - chr4.2:84656048-84656067 MS.gene70044:intron 40.0%
AAATCCATATAACCCTGGAG+TGG + chr4.2:84656269-84656288 None:intergenic 40.0%
AAGTAAGTCACAGAGGAACT+TGG + chr4.2:84655466-84655485 None:intergenic 40.0%
ACACAACATTGGCAAACAAG+AGG - chr4.2:84655540-84655559 MS.gene70044:intron 40.0%
ACACACCCATCAAAAGAGTT+AGG + chr4.2:84656664-84656683 None:intergenic 40.0%
AGAGGTTTCTGATGTTGTCA+AGG + chr4.2:84655153-84655172 None:intergenic 40.0%
AGCTTTACAATGGACCCTAA+TGG + chr4.2:84656634-84656653 None:intergenic 40.0%
AGGTCCCAAAAGCTTTACAA+TGG + chr4.2:84656644-84656663 None:intergenic 40.0%
ATCCAATTTCCTGTTGTCAC+TGG + chr4.2:84656518-84656537 None:intergenic 40.0%
ATTTGTCAAAGGCTCATGAG+AGG + chr4.2:84656416-84656435 None:intergenic 40.0%
CAATGCTTCACATGGATTTC+TGG + chr4.2:84655248-84655267 None:intergenic 40.0%
CCTAAGAACTTGGATTCTGA+GGG - chr4.2:84655358-84655377 MS.gene70044:intron 40.0%
CTCTGTGATCACAACTTAGT+TGG - chr4.2:84655664-84655683 MS.gene70044:intron 40.0%
GACTTACTTGAAGCTGTTGA+TGG - chr4.2:84655477-84655496 MS.gene70044:intron 40.0%
GCATAGCAACATGTCGTAAT+TGG - chr4.2:84655697-84655716 MS.gene70044:intron 40.0%
GTGACCTTACTCTAATATGG+TGG + chr4.2:84656157-84656176 None:intergenic 40.0%
GTGATCACAACTTAGTTGGT+AGG - chr4.2:84655668-84655687 MS.gene70044:intron 40.0%
GTTCTTAGGCTGTCTATTAG+TGG + chr4.2:84655347-84655366 None:intergenic 40.0%
TGAGAGTAATGCCCAAAATG+AGG - chr4.2:84655384-84655403 MS.gene70044:intron 40.0%
TTCATGCACAATGCTTCACA+TGG + chr4.2:84655256-84655275 None:intergenic 40.0%
! AGGGTCCATTGTAAAGCTTT+TGG - chr4.2:84656636-84656655 MS.gene70044:CDS 40.0%
! CTTGAAGCTGTTGATGGAAT+TGG - chr4.2:84655483-84655502 MS.gene70044:intron 40.0%
! GGAATTGGGGTATTCAGATT+TGG - chr4.2:84655498-84655517 MS.gene70044:intron 40.0%
! GGGTCCATTGTAAAGCTTTT+GGG - chr4.2:84656637-84656656 MS.gene70044:CDS 40.0%
! TGAAGCTGTTGATGGAATTG+GGG - chr4.2:84655485-84655504 MS.gene70044:intron 40.0%
! TGGGACCTAACTCTTTTGAT+GGG - chr4.2:84656656-84656675 MS.gene70044:CDS 40.0%
! TTGGGACCTAACTCTTTTGA+TGG - chr4.2:84656655-84656674 MS.gene70044:CDS 40.0%
!! AACTCTTTTGATGGGTGTGT+TGG - chr4.2:84656664-84656683 MS.gene70044:CDS 40.0%
!! TGTTTTTGTGGAAGCAACCA+AGG - chr4.2:84655012-84655031 MS.gene70044:CDS 40.0%
!!! TCTATTAGTGGGTTTTCCAC+AGG + chr4.2:84655335-84655354 None:intergenic 40.0%
ACTTCCACTCCAGGGTTATA+TGG - chr4.2:84656262-84656281 MS.gene70044:CDS 45.0%
AGCTTCCTATCTCTTCCACT+TGG - chr4.2:84655055-84655074 MS.gene70044:intron 45.0%
AGCTTCCTTACCATGCCATT+AGG - chr4.2:84656616-84656635 MS.gene70044:CDS 45.0%
AGGATCGAGTAACACAGACT+TGG + chr4.2:84656722-84656741 None:intergenic 45.0%
AGGTCGGTTGTCTTTGTTCT+TGG + chr4.2:84656458-84656477 None:intergenic 45.0%
CAAAGGCTCATGAGAGGTTA+AGG + chr4.2:84656410-84656429 None:intergenic 45.0%
CACCAGTGACAACAGGAAAT+TGG - chr4.2:84656513-84656532 MS.gene70044:CDS 45.0%
CAGAATCTTGATTGCACCTG+TGG - chr4.2:84655316-84655335 MS.gene70044:intron 45.0%
CAGCTTCAAGTAAGTCACAG+AGG + chr4.2:84655473-84655492 None:intergenic 45.0%
CCCTCAGAATCCAAGTTCTT+AGG + chr4.2:84655361-84655380 None:intergenic 45.0%
CTCTAACTGCTGATGCAAGT+TGG + chr4.2:84656483-84656502 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG - chr4.2:84655529-84655548 MS.gene70044:intron 45.0%
GATCGAGTAACACAGACTTG+GGG + chr4.2:84656720-84656739 None:intergenic 45.0%
GCCTAAGAACTTGGATTCTG+AGG - chr4.2:84655357-84655376 MS.gene70044:intron 45.0%
GCTTCCTTACCATGCCATTA+GGG - chr4.2:84656617-84656636 MS.gene70044:CDS 45.0%
GGATCGAGTAACACAGACTT+GGG + chr4.2:84656721-84656740 None:intergenic 45.0%
TACAATGGACCCTAATGGCA+TGG + chr4.2:84656629-84656648 None:intergenic 45.0%
! ACTGAAGCATCTCTTCTCAG+AGG - chr4.2:84656238-84656257 MS.gene70044:CDS 45.0%
! GTTGTTGTTATTCCTGGTGG+TGG + chr4.2:84655101-84655120 None:intergenic 45.0%
!! ACTGCTGATGCAAGTTGGTT+AGG + chr4.2:84656478-84656497 None:intergenic 45.0%
!! CTGATGCAAGTTGGTTAGGT+CGG + chr4.2:84656474-84656493 None:intergenic 45.0%
!! GAGAGTGTCAACAAAGTCCT+TGG + chr4.2:84655032-84655051 None:intergenic 45.0%
!! TATTCCTGGTGGTGGCTAAA+AGG + chr4.2:84655093-84655112 None:intergenic 45.0%
!!! TATTATTTGATATTTTATAA+TGG - chr4.2:84655967-84655986 MS.gene70044:intron 5.0%
CATGAGAGGTTAAGGTGTGC+TGG + chr4.2:84656402-84656421 None:intergenic 50.0%
GGCTAGAGTCTCACCCTTTA+AGG + chr4.2:84655753-84655772 None:intergenic 50.0%
TCAGAGGAACTTCCACTCCA+GGG - chr4.2:84656254-84656273 MS.gene70044:CDS 50.0%
TGGACCCTAATGGCATGGTA+AGG + chr4.2:84656624-84656643 None:intergenic 50.0%
TTCCTGTTGTCACTGGTGCA+AGG + chr4.2:84656511-84656530 None:intergenic 50.0%
! GATTTTGGACGGTACGGTAG+AGG + chr4.2:84655171-84655190 None:intergenic 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG + chr4.2:84655063-84655082 None:intergenic 50.0%
AGGGTGAGACTCTAGCCACT+TGG - chr4.2:84655756-84655775 MS.gene70044:intron 55.0%
AGGTTAAGGTGTGCTGGAGG+AGG + chr4.2:84656396-84656415 None:intergenic 55.0%
ATCCTGGCACAAGGTGTGCA+AGG - chr4.2:84656057-84656076 MS.gene70044:intron 55.0%
ATCCTTGCACACCTTGTGCC+AGG + chr4.2:84656062-84656081 None:intergenic 55.0%
CTCAGAGGAACTTCCACTCC+AGG - chr4.2:84656253-84656272 MS.gene70044:CDS 55.0%
GAGAGGTTAAGGTGTGCTGG+AGG + chr4.2:84656399-84656418 None:intergenic 55.0%
GGTGGCTAAAAGGCGAACGA+TGG + chr4.2:84655083-84655102 None:intergenic 55.0%
TGCCTTGCACCAGTGACAAC+AGG - chr4.2:84656506-84656525 MS.gene70044:CDS 55.0%
! TTCGCCTTTTAGCCACCACC+AGG - chr4.2:84655086-84655105 MS.gene70044:intron 55.0%
! AGGCGAACGATGGTGCCAAG+TGG + chr4.2:84655073-84655092 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84654935 84656759 84654935 ID=MS.gene70044
chr4.2 mRNA 84654935 84656759 84654935 ID=MS.gene70044.t1;Parent=MS.gene70044
chr4.2 exon 84656133 84656759 84656133 ID=MS.gene70044.t1.exon1;Parent=MS.gene70044.t1
chr4.2 CDS 84656133 84656759 84656133 ID=cds.MS.gene70044.t1;Parent=MS.gene70044.t1
chr4.2 exon 84655141 84655309 84655141 ID=MS.gene70044.t1.exon2;Parent=MS.gene70044.t1
chr4.2 CDS 84655141 84655309 84655141 ID=cds.MS.gene70044.t1;Parent=MS.gene70044.t1
chr4.2 exon 84654935 84655056 84654935 ID=MS.gene70044.t1.exon3;Parent=MS.gene70044.t1
chr4.2 CDS 84654935 84655056 84654935 ID=cds.MS.gene70044.t1;Parent=MS.gene70044.t1
Gene Sequence

>MS.gene70044

ATGGCTTCTGAACAGCTACAAAAAGATAAAAGAACAATTCCCCTCAAAATGTTAGTGGACAAACAAAGGAACAAAGTTGTTTTTGTGGAAGCAACCAAGGACTTTGTTGACACTCTCTTCAGCTTCCTATCTCTTCCACTTGGCACCATCGTTCGCCTTTTAGCCACCACCAGGAATAACAACAACAATGACCAACAATCAGAATCAATACCATTCCTTGACAACATCAGAAACCTCTACCGTACCGTCCAAAATCTCAATTCAAATGATGTTTGGAACAATCCTGTCTGCAAACAAATGCTTTTGCATCCCAGAAATCCATGTGAAGCATTGTGCATGAAGCTGTTCTTGAATGTTGATGACACTGAGTTTACTACTTTTCAGAATCTTGATTGCACCTGTGGAAAACCCACTAATAGACAGCCTAAGAACTTGGATTCTGAGGGTCATGAGAGTAATGCCCAAAATGAGGTCTTTATAAGAGAAAATGGATCAATGTTTCTGGTGTCTGATGATTTGAAGATTGTGCCAAGTTCCTCTGTGACTTACTTGAAGCTGTTGATGGAATTGGGGTATTCAGATTTGGCTCAAATAGAGGAAGTCACACACAACATTGGCAAACAAGAGGTAATGCTTTTTGTGTTTGATCTGTATTACATTGAATTTGTTAATTAATATTCACAATCATTTGGATGTCATTTTTTACAAGCAAAAGAGAATATATTAAATCTCTGTGATCACAACTTAGTTGGTAGGGACAACGCATAGCAACATGTCGTAATTGGTGTTTGAACCTTGAATTTACTGCTTATTCACCTTAAAGGGTGAGACTCTAGCCACTTGGCTAATTGACAAAAATAAAGAAATACTATGGGCATTAGTACTTCAACCCAATACAAGAAAAAGCAATACTATGGGCATGTTTCGATTAACTTTTAGTTGAAATCTAAAGGCATAAGTACCGATAAGAATGTTTGGTAAATGTTATGAAAGTAGCTTATGACAAATCTATAAGAATGTTTTCCATACGACTATTATTTGATATTTTATAATGGATTAATTTGAGAGAGTATACAAAGTATGAGAGAGTATAAGGATGTTTGAGTTAATTAAAAAAATTGCATCCTGGCACAAGGTGTGCAAGGATGACAGAGTATACAAAGTAATATTCAAAAAGAGCATTAAAACTAAAAAAATATCAGTACTAAAGATTACAACACCACCATATTAGAGTAAGGTCACTAGATGAGTAGATAAGATCGTTCTGCATTTTTTTGTTAGAAAACATGTGGTTTCAGCGGTTACTGAAGCATCTCTTCTCAGAGGAACTTCCACTCCAGGGTTATATGGATTTCCTTTCTTTAATAGATCTATTTTCAATAAATAATATAATTTATTCTTCTTTGTTGTATTGTTATGCTTACTATTTGTGTTTCAACATATTCTATAGATATTGAACCTCCTCCAGCACACCTTAACCTCTCATGAGCCTTTGACAAATACATTTTTTGCAAGTAGCTCCAAGAACAAAGACAACCGACCTAACCAACTTGCATCAGCAGTTAGAGCAATGCCTTGCACCAGTGACAACAGGAAATTGGATGTTAAAGTAATTCAAAGTAAATCTCAAAAAAATATAATCTTTGCAGAAACCAATGGAGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTAGGGTCCATTGTAAAGCTTTTGGGACCTAACTCTTTTGATGGGTGTGTTGGTAATTTGTACAATAGTGTGAAGAAGTTGGACCCCAAGTCTGTGTTACTCGATCCTCGTATAGCACACCTGTGA
Protein sequence

>MS.gene70044.t1

MASEQLQKDKRTIPLKMLVDKQRNKVVFVEATKDFVDTLFSFLSLPLGTIVRLLATTRNNNNNDQQSESIPFLDNIRNLYRTVQNLNSNDVWNNPVCKQMLLHPRNPCEALCMKLFLNVDDTEFTTFQNLDCTCGKPTNRQPKNLDSEGHESNAQNEVFIRENGSMFLVSDDLKIVPSSSVTYLKLLMELGYSDLAQIEEVTHNIGKQEILNLLQHTLTSHEPLTNTFFASSSKNKDNRPNQLASAVRAMPCTSDNRKLDVKVIQRSIVKLLGPNSFDGCVGNLYNSVKKLDPKSVLLDPRIAHL