AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene99478

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99478.t1 MTR_4g019340 87.454 271 11 2 1 271 256 503 6.09e-165 469
MS.gene99478.t1 MTR_4g017830 72.026 311 86 1 1 310 220 530 9.17e-154 441
MS.gene99478.t1 MTR_4g017470 66.667 294 94 2 1 290 186 479 1.18e-128 376
MS.gene99478.t1 MTR_4g017470 26.587 252 110 6 44 277 10 204 5.12e-11 63.5
MS.gene99478.t1 MTR_4g017440 69.065 278 80 3 1 277 226 498 2.66e-126 370
MS.gene99478.t1 MTR_4g017550 64.211 285 90 6 1 281 197 473 6.12e-111 330
MS.gene99478.t1 MTR_4g017410 60.269 297 83 5 1 290 225 493 2.30e-104 314
MS.gene99478.t1 MTR_4g017610 54.152 277 103 6 1 277 223 475 2.69e-86 267
MS.gene99478.t1 MTR_4g019310 41.463 287 135 7 1 277 223 486 3.01e-55 187
MS.gene99478.t1 MTR_4g019310 27.984 243 147 6 48 274 8 238 1.12e-14 74.7
MS.gene99478.t1 MTR_7g066400 34.828 290 144 7 1 284 190 440 5.94e-36 134
MS.gene99478.t1 MTR_7g066310 34.211 266 119 5 48 310 193 405 2.20e-35 132
MS.gene99478.t1 MTR_7g066310 33.636 110 65 3 48 150 7 115 8.11e-11 62.8
MS.gene99478.t1 MTR_7g066570 33.219 292 172 6 1 285 209 484 2.38e-34 132
MS.gene99478.t1 MTR_7g066340 32.166 314 155 8 1 310 216 475 1.12e-33 129
MS.gene99478.t1 MTR_7g066340 29.046 241 136 8 48 274 12 231 7.36e-17 81.3
MS.gene99478.t1 MTR_7g066410 33.108 296 167 7 1 281 233 512 3.27e-32 125
MS.gene99478.t1 MTR_7g066350 32.095 296 140 7 1 284 211 457 1.15e-31 123
MS.gene99478.t1 MTR_7g066350 28.685 251 150 8 48 290 9 238 5.73e-17 81.3
MS.gene99478.t1 MTR_7g066260 31.646 316 167 8 1 310 219 491 1.43e-31 123
MS.gene99478.t1 MTR_7g066260 29.237 236 142 7 48 274 15 234 1.04e-14 74.7
MS.gene99478.t1 MTR_7g058710 29.932 294 183 4 1 287 206 483 4.78e-31 121
MS.gene99478.t1 MTR_4g019240 88.571 70 8 0 240 309 113 182 5.03e-31 115
MS.gene99478.t1 MTR_7g062280 33.106 293 165 8 1 287 224 491 7.84e-31 121
MS.gene99478.t1 MTR_7g062280 30.000 260 135 12 49 285 11 246 7.67e-15 75.1
MS.gene99478.t1 MTR_7g066580 31.707 287 174 6 1 279 167 439 1.51e-30 120
MS.gene99478.t1 MTR_7g066430 34.859 284 173 6 1 280 209 484 2.18e-30 120
MS.gene99478.t1 MTR_7g066280 31.636 275 158 7 51 310 153 412 3.92e-29 115
MS.gene99478.t1 MTR_1g041315 31.985 272 131 9 1 265 227 451 6.29e-29 115
MS.gene99478.t1 MTR_7g066680 30.818 318 158 9 1 277 38 334 2.08e-27 109
MS.gene99478.t1 MTR_7g012680 32.534 292 153 7 1 285 195 449 2.30e-27 111
MS.gene99478.t1 MTR_7g012680 26.506 249 135 8 36 275 2 211 7.62e-12 65.9
MS.gene99478.t1 MTR_7g063920 29.795 292 170 7 1 287 175 436 2.68e-27 110
MS.gene99478.t1 MTR_4g019350 52.000 100 37 2 106 205 1 89 6.33e-25 96.3
MS.gene99478.t1 MTR_7g063840 29.758 289 178 7 1 287 208 473 8.95e-25 103
MS.gene99478.t1 MTR_7g063880 30.584 291 164 8 1 286 210 467 9.67e-25 103
MS.gene99478.t1 MTR_7g063880 28.395 243 130 9 48 274 11 225 7.36e-14 72.0
MS.gene99478.t1 MTR_7g062300 32.042 284 158 10 1 277 214 469 1.80e-24 103
MS.gene99478.t1 MTR_7g062300 28.685 251 138 11 49 285 13 236 2.68e-13 70.5
MS.gene99478.t1 MTR_7g066360 30.945 307 148 9 14 311 228 479 3.17e-24 102
MS.gene99478.t1 MTR_7g063720 30.864 243 132 5 47 286 22 231 8.62e-24 97.8
MS.gene99478.t1 MTR_7g063900 32.632 285 176 7 1 281 210 482 2.25e-22 97.4
MS.gene99478.t1 MTR_7g063900 28.514 249 141 8 48 285 10 232 2.07e-15 77.0
MS.gene99478.t1 MTR_1g014060 35.749 207 98 5 78 281 210 384 2.63e-22 96.3
MS.gene99478.t1 MTR_8g445860 29.249 253 121 7 48 275 13 232 2.72e-22 93.6
MS.gene99478.t1 MTR_7g063660 29.866 298 171 8 1 287 207 477 1.46e-21 95.1
MS.gene99478.t1 MTR_7g063660 36.066 122 69 3 48 162 9 128 4.50e-15 75.9
MS.gene99478.t1 MTR_7g063820 30.405 296 174 7 1 287 212 484 7.98e-21 92.8
MS.gene99478.t1 MTR_7g063820 26.971 241 151 6 42 274 4 227 7.17e-13 69.3
MS.gene99478.t1 MTR_1g115440 30.705 241 136 8 48 275 11 233 8.25e-21 89.7
MS.gene99478.t1 MTR_7g063670 29.861 288 163 8 1 287 208 457 9.84e-21 92.4
MS.gene99478.t1 MTR_0150s0010 30.508 236 143 6 48 275 10 232 1.72e-20 88.6
MS.gene99478.t1 MTR_7g063850 29.317 249 145 7 46 287 64 288 2.60e-20 89.4
MS.gene99478.t1 MTR_3g065200 28.632 234 137 5 45 275 209 415 3.83e-20 90.9
MS.gene99478.t1 MTR_7g100420 32.258 279 167 6 1 277 135 393 1.21e-19 89.0
MS.gene99478.t1 MTR_2g029400 27.798 277 152 8 1 269 167 403 3.84e-18 84.7
MS.gene99478.t1 MTR_7g063830 27.119 295 149 7 1 288 210 445 2.29e-17 82.4
MS.gene99478.t1 MTR_7g063890 28.390 236 133 7 51 274 30 241 6.16e-15 75.5
MS.gene99478.t1 MTR_7g063890 28.664 307 173 10 1 288 226 505 2.31e-14 73.6
MS.gene99478.t1 MTR_0150s0030 35.294 102 57 2 48 146 8 103 2.70e-14 70.9
MS.gene99478.t1 MTR_8g445910 36.275 102 56 2 48 146 8 103 3.88e-14 71.6
MS.gene99478.t1 MTR_7g066290 34.074 135 75 4 14 139 232 361 4.24e-14 72.8
MS.gene99478.t1 MTR_7g058700 33.835 133 65 3 1 130 725 837 5.73e-14 72.8
MS.gene99478.t1 MTR_7g066390 43.137 102 55 2 209 310 50 148 9.71e-14 68.2
MS.gene99478.t1 MTR_1g115450 40.506 79 45 1 48 124 7 85 2.56e-13 66.6
MS.gene99478.t1 MTR_8g044880 36.275 102 56 2 48 146 8 103 2.72e-13 69.3
MS.gene99478.t1 MTR_8g445920 43.548 62 35 0 48 109 10 71 9.66e-12 61.2
MS.gene99478.t1 MTR_8g044890 43.548 62 35 0 48 109 10 71 9.66e-12 61.2
MS.gene99478.t1 MTR_1g115455 37.975 79 47 1 48 124 9 87 1.02e-11 63.9
MS.gene99478.t1 MTR_7g066480 35.922 103 55 1 190 281 319 421 3.62e-11 63.9
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene99478.t1 AT5G01120 29.430 316 186 10 4 310 220 507 1.81e-22 97.8
MS.gene99478.t1 AT5G01140 29.299 314 184 9 1 310 193 472 9.65e-22 95.5
MS.gene99478.t1 AT3G09140 29.568 301 149 9 1 277 228 489 4.59e-20 90.9
MS.gene99478.t1 AT3G09140 27.049 244 160 9 44 274 5 243 5.00e-13 69.7
MS.gene99478.t1 AT3G09140 29.568 301 149 9 1 277 256 517 4.92e-20 90.9
MS.gene99478.t1 AT5G01130 27.957 279 156 7 1 277 205 440 5.23e-20 90.5
MS.gene99478.t1 AT5G43240 28.956 297 158 10 4 277 79 345 3.04e-15 75.9
MS.gene99478.t1 AT5G43240 28.956 297 158 10 4 277 223 489 4.07e-15 76.3
MS.gene99478.t1 AT5G43240 28.956 297 158 10 4 277 223 489 4.07e-15 76.3
MS.gene99478.t1 AT5G43240 28.956 297 158 10 4 277 223 489 4.07e-15 76.3
MS.gene99478.t1 AT5G01150 25.483 259 158 6 57 310 272 500 4.19e-15 75.9
MS.gene99478.t1 AT3G09120 27.004 237 151 7 48 275 6 229 3.69e-13 69.3

Find 64 sgRNAs with CRISPR-Local

Find 106 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCGGGTTGTCTTTGTTCT+TGG 0.234472 4.2:-83136605 None:intergenic
ATAACTCAGTCTTCTAGTTT+AGG 0.304871 4.2:-83137917 None:intergenic
CCACTCACTACATATTATTA+TGG 0.320058 4.2:+83136967 MS.gene99478:CDS
AGGGTCCATTGTAAAGGTTT+TGG 0.343635 4.2:+83136783 MS.gene99478:CDS
AGCTTCCTTACAATACCATT+AGG 0.351633 4.2:+83136763 MS.gene99478:CDS
TTGCACCCGAGACATTAAAA+TGG 0.365960 4.2:+83136660 MS.gene99478:CDS
ACTGCTGATGCAAACTGGTT+TGG 0.367245 4.2:-83136625 None:intergenic
AGCAAAAGCCTTGACTGAAA+TGG 0.377248 4.2:+83137098 MS.gene99478:CDS
GCTTCCTTACAATACCATTA+GGG 0.381396 4.2:+83136764 MS.gene99478:CDS
CTTTGACTGCTGATGCAAAC+TGG 0.388699 4.2:-83136630 None:intergenic
GGAATGTTGAGGGGCTGGTT+TGG 0.395287 4.2:-83136931 None:intergenic
CTATAATTATACTCTTGCTT+TGG 0.402657 4.2:-83137000 None:intergenic
CAGAGGCTCATGAGAGGTTA+AGG 0.420057 4.2:-83136557 None:intergenic
AACGGTAGAATTAAAGATAG+AGG 0.436841 4.2:+83137038 MS.gene99478:CDS
ACTAGTTTGAACACACTTTG+AGG 0.444744 4.2:-83137870 None:intergenic
GAGTATAATTATAGTTATCA+TGG 0.454252 4.2:+83137009 MS.gene99478:CDS
ACCATTAGGGTCCATTGTAA+AGG 0.460196 4.2:+83136777 MS.gene99478:CDS
CGCTCCATGTTACTCAATCC+TGG 0.462371 4.2:+83136886 MS.gene99478:CDS
ACCTTTACAATGGACCCTAA+TGG 0.477568 4.2:-83136778 None:intergenic
CATGAGGAATGTTGAGGGGC+TGG 0.489637 4.2:-83136936 None:intergenic
GCTGATGCAAACTGGTTTGG+CGG 0.490283 4.2:-83136622 None:intergenic
CAAAGTGTGTTCAAACTAGT+AGG 0.500953 4.2:+83137873 MS.gene99478:CDS
GTACAAGAGTGTAGAGAGCT+TGG 0.505497 4.2:+83136840 MS.gene99478:CDS
TCTTGGGTCCATTTCAGTCA+AGG 0.507464 4.2:-83137106 None:intergenic
ACTACATATTATTATGGTAC+AGG 0.518635 4.2:+83136973 MS.gene99478:CDS
TTCAAAATTGTATTTGTCAG+AGG 0.520812 4.2:-83136574 None:intergenic
TTAATTCTTTCAAATATGAA+AGG 0.523699 4.2:-83137233 None:intergenic
TGGACCCTAATGGTATTGTA+AGG 0.526033 4.2:-83136768 None:intergenic
TCAAAGTGTGGTGCGACACC+AGG 0.529733 4.2:-83136904 None:intergenic
AGGCAAGAATTAGCAGAAAG+TGG 0.533853 4.2:-83137213 None:intergenic
CCTTGTTGATCTTGAAGTGA+AGG 0.535507 4.2:+83137263 MS.gene99478:CDS
TAGTTATCATGGTTATCAAA+CGG 0.536461 4.2:+83137020 MS.gene99478:CDS
TCAAAATTTACACTGACTAG+TGG 0.539589 4.2:+83137803 MS.gene99478:CDS
CTGATGCAAACTGGTTTGGC+GGG 0.543289 4.2:-83136621 None:intergenic
CTTCAAGATCAACAAGGGGA+AGG 0.544062 4.2:-83137257 None:intergenic
ATTTACACTGACTAGTGGAC+TGG 0.547720 4.2:+83137808 MS.gene99478:CDS
TAGAGAGCTTGGACTCTTCG+TGG 0.547747 4.2:+83136851 MS.gene99478:CDS
GGAGTGATTTCTAAGTCGCA+TGG 0.571812 4.2:+83137063 MS.gene99478:CDS
GAAGTCAACTAAGTTTCCAC+TGG 0.577621 4.2:-83136734 None:intergenic
GTAGAATTAAAGATAGAGGG+AGG 0.580072 4.2:+83137042 MS.gene99478:CDS
ACGGTAGAATTAAAGATAGA+GGG 0.581849 4.2:+83137039 MS.gene99478:CDS
ACCATCCAAAACCTTTACAA+TGG 0.583435 4.2:-83136788 None:intergenic
TTTCCTACACTATATAATGC+CGG 0.585136 4.2:-83137174 None:intergenic
CCTTCACTTCAAGATCAACA+AGG 0.610416 4.2:-83137263 None:intergenic
AGGTTAAGGTGTACTTGAGG+AGG 0.616375 4.2:-83136543 None:intergenic
ATGTACATGAGGAATGTTGA+GGG 0.622021 4.2:-83136941 None:intergenic
GTGAGTGGTGGATGTACATG+AGG 0.635076 4.2:-83136952 None:intergenic
GATAAGCATTGGTGAAGCTG+AGG 0.638424 4.2:+83137287 MS.gene99478:CDS
TTGATGTCAAGGAAGCTGCA+AGG 0.648287 4.2:-83137784 None:intergenic
GATGGGATTTGTGAAGAGAC+CGG 0.651602 4.2:+83137155 MS.gene99478:CDS
CTTCACTTCAAGATCAACAA+GGG 0.654366 4.2:-83137262 None:intergenic
GAAGTGAAGGTGATAAGCAT+TGG 0.654807 4.2:+83137276 MS.gene99478:CDS
TAATAATATGTAGTGAGTGG+TGG 0.656097 4.2:-83136964 None:intergenic
AGACCGGCATTATATAGTGT+AGG 0.657855 4.2:+83137171 MS.gene99478:CDS
TGGTTTGGACAATCAAAGTG+TGG 0.662188 4.2:-83136916 None:intergenic
GAGAGGTTAAGGTGTACTTG+AGG 0.667782 4.2:-83136546 None:intergenic
ATAATCTGTGCAGAAGCCAG+TGG 0.673897 4.2:+83136718 MS.gene99478:CDS
ATTTGTCAGAGGCTCATGAG+AGG 0.675408 4.2:-83136563 None:intergenic
TTCACTTCAAGATCAACAAG+GGG 0.676098 4.2:-83137261 None:intergenic
GATGTACATGAGGAATGTTG+AGG 0.679220 4.2:-83136942 None:intergenic
ATGTCAAGGAAGCTGCAAGG+AGG 0.721178 4.2:-83137781 None:intergenic
CCATAATAATATGTAGTGAG+TGG 0.727149 4.2:-83136967 None:intergenic
GACACCAGGATTGAGTAACA+TGG 0.730789 4.2:-83136890 None:intergenic
TGTACATGAGGAATGTTGAG+GGG 0.757600 4.2:-83136940 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTAAAAAAAGATGTGAT+GGG + chr4.2:83137138-83137157 MS.gene99478:CDS 15.0%
!! TAATAAAATTATTGGACTTA+TGG - chr4.2:83137498-83137517 None:intergenic 15.0%
!! TTAATTCTTTCAAATATGAA+AGG - chr4.2:83137236-83137255 None:intergenic 15.0%
!!! ACATCTTTTTTTAATTTGTT+GGG - chr4.2:83137135-83137154 None:intergenic 15.0%
!! CAAATTAAAAAAAGATGTGA+TGG + chr4.2:83137137-83137156 MS.gene99478:CDS 20.0%
!! GAGTATAATTATAGTTATCA+TGG + chr4.2:83137009-83137028 MS.gene99478:CDS 20.0%
!! TTAGACAACAAATAAACAAA+AGG - chr4.2:83137350-83137369 None:intergenic 20.0%
!!! ATCTTTTTTTAATTTGTTGG+GGG - chr4.2:83137133-83137152 None:intergenic 20.0%
!!! CACATCTTTTTTTAATTTGT+TGG - chr4.2:83137136-83137155 None:intergenic 20.0%
!!! CATCTTTTTTTAATTTGTTG+GGG - chr4.2:83137134-83137153 None:intergenic 20.0%
! AAATTATTGGACTTATGGAA+AGG - chr4.2:83137493-83137512 None:intergenic 25.0%
! ACTACATATTATTATGGTAC+AGG + chr4.2:83136973-83136992 MS.gene99478:CDS 25.0%
! CTATAATTATACTCTTGCTT+TGG - chr4.2:83137003-83137022 None:intergenic 25.0%
! CTCAAATACATGTTATGATT+GGG + chr4.2:83137549-83137568 MS.gene99478:intron 25.0%
! TAGTTATCATGGTTATCAAA+CGG + chr4.2:83137020-83137039 MS.gene99478:CDS 25.0%
! TCTCAAATACATGTTATGAT+TGG + chr4.2:83137548-83137567 MS.gene99478:intron 25.0%
! TTCAAAATTGTATTTGTCAG+AGG - chr4.2:83136577-83136596 None:intergenic 25.0%
AAATACATGTTATGATTGGG+TGG + chr4.2:83137552-83137571 MS.gene99478:intron 30.0%
AACGGTAGAATTAAAGATAG+AGG + chr4.2:83137038-83137057 MS.gene99478:CDS 30.0%
AATACATGTTATGATTGGGT+GGG + chr4.2:83137553-83137572 MS.gene99478:intron 30.0%
ACGGTAGAATTAAAGATAGA+GGG + chr4.2:83137039-83137058 MS.gene99478:CDS 30.0%
CCACTCACTACATATTATTA+TGG + chr4.2:83136967-83136986 MS.gene99478:CDS 30.0%
CCATAATAATATGTAGTGAG+TGG - chr4.2:83136970-83136989 None:intergenic 30.0%
TAATAATATGTAGTGAGTGG+TGG - chr4.2:83136967-83136986 None:intergenic 30.0%
TTTCCTACACTATATAATGC+CGG - chr4.2:83137177-83137196 None:intergenic 30.0%
! TCAAAATTTACACTGACTAG+TGG + chr4.2:83137803-83137822 MS.gene99478:CDS 30.0%
!! CAGTGTAAATTTTGATGTCA+AGG - chr4.2:83137798-83137817 None:intergenic 30.0%
!! TAACGTCCATTTTAATGTCT+CGG - chr4.2:83136669-83136688 None:intergenic 30.0%
AAATCAAGAGGTAAGAATCC+TGG - chr4.2:83137742-83137761 None:intergenic 35.0%
ACCATCCAAAACCTTTACAA+TGG - chr4.2:83136791-83136810 None:intergenic 35.0%
AGCTTCCTTACAATACCATT+AGG + chr4.2:83136763-83136782 MS.gene99478:CDS 35.0%
ATACATGTTATGATTGGGTG+GGG + chr4.2:83137554-83137573 MS.gene99478:intron 35.0%
ATGTACATGAGGAATGTTGA+GGG - chr4.2:83136944-83136963 None:intergenic 35.0%
CAAAGTGTGTTCAAACTAGT+AGG + chr4.2:83137873-83137892 MS.gene99478:CDS 35.0%
CTTCACTTCAAGATCAACAA+GGG - chr4.2:83137265-83137284 None:intergenic 35.0%
GCTTCCTTACAATACCATTA+GGG + chr4.2:83136764-83136783 MS.gene99478:CDS 35.0%
GTAGAATTAAAGATAGAGGG+AGG + chr4.2:83137042-83137061 MS.gene99478:CDS 35.0%
TATCAGATGCGTATCAGTAT+CGG - chr4.2:83137460-83137479 None:intergenic 35.0%
TCATATCATACTGTGTATCC+AGG + chr4.2:83137721-83137740 MS.gene99478:intron 35.0%
TTCAACATGACACAGATATG+AGG - chr4.2:83137700-83137719 None:intergenic 35.0%
TTCACTTCAAGATCAACAAG+GGG - chr4.2:83137264-83137283 None:intergenic 35.0%
TTCGAATGCTATACAGATCT+TGG + chr4.2:83137663-83137682 MS.gene99478:intron 35.0%
! ACTAGTTTGAACACACTTTG+AGG - chr4.2:83137873-83137892 None:intergenic 35.0%
! TTGGACTTATGGAAAGGTAT+TGG - chr4.2:83137487-83137506 None:intergenic 35.0%
! TTGGATGGTAACTCTTTTGT+TGG + chr4.2:83136802-83136821 MS.gene99478:CDS 35.0%
!! AACGTCCATTTTAATGTCTC+GGG - chr4.2:83136668-83136687 None:intergenic 35.0%
!! TGGACTTATGGAAAGGTATT+GGG - chr4.2:83137486-83137505 None:intergenic 35.0%
!! TTAATTTGTTGGGGGATCTT+GGG - chr4.2:83137125-83137144 None:intergenic 35.0%
!! TTTAATTTGTTGGGGGATCT+TGG - chr4.2:83137126-83137145 None:intergenic 35.0%
!!! TCCATTGTAAAGGTTTTGGA+TGG + chr4.2:83136787-83136806 MS.gene99478:CDS 35.0%
ACCATTAGGGTCCATTGTAA+AGG + chr4.2:83136777-83136796 MS.gene99478:CDS 40.0%
ACCTTTACAATGGACCCTAA+TGG - chr4.2:83136781-83136800 None:intergenic 40.0%
AGACCGGCATTATATAGTGT+AGG + chr4.2:83137171-83137190 MS.gene99478:CDS 40.0%
AGCAAAAGCCTTGACTGAAA+TGG + chr4.2:83137098-83137117 MS.gene99478:CDS 40.0%
AGGCAAGAATTAGCAGAAAG+TGG - chr4.2:83137216-83137235 None:intergenic 40.0%
CCTGCAAATTGCAAATCAAG+AGG - chr4.2:83137754-83137773 None:intergenic 40.0%
CCTTCACTTCAAGATCAACA+AGG - chr4.2:83137266-83137285 None:intergenic 40.0%
CCTTGTTGATCTTGAAGTGA+AGG + chr4.2:83137263-83137282 MS.gene99478:CDS 40.0%
CTATGCTTCATAGACCACAT+GGG - chr4.2:83137397-83137416 None:intergenic 40.0%
GAAGTCAACTAAGTTTCCAC+TGG - chr4.2:83136737-83136756 None:intergenic 40.0%
GAAGTGAAGGTGATAAGCAT+TGG + chr4.2:83137276-83137295 MS.gene99478:CDS 40.0%
GATGTACATGAGGAATGTTG+AGG - chr4.2:83136945-83136964 None:intergenic 40.0%
TACATGTTATGATTGGGTGG+GGG + chr4.2:83137555-83137574 MS.gene99478:intron 40.0%
TCTATGCTTCATAGACCACA+TGG - chr4.2:83137398-83137417 None:intergenic 40.0%
TGGACCCTAATGGTATTGTA+AGG - chr4.2:83136771-83136790 None:intergenic 40.0%
TGTACATGAGGAATGTTGAG+GGG - chr4.2:83136943-83136962 None:intergenic 40.0%
TTGCACCCGAGACATTAAAA+TGG + chr4.2:83136660-83136679 MS.gene99478:CDS 40.0%
! AGGGTCCATTGTAAAGGTTT+TGG + chr4.2:83136783-83136802 MS.gene99478:CDS 40.0%
! ATTTACACTGACTAGTGGAC+TGG + chr4.2:83137808-83137827 MS.gene99478:CDS 40.0%
! TGGTTTGGACAATCAAAGTG+TGG - chr4.2:83136919-83136938 None:intergenic 40.0%
!! ACTTATGGAAAGGTATTGGG+GGG - chr4.2:83137483-83137502 None:intergenic 40.0%
!! CCTCTTGATTTGCAATTTGC+AGG + chr4.2:83137751-83137770 MS.gene99478:intron 40.0%
!! GACTTATGGAAAGGTATTGG+GGG - chr4.2:83137484-83137503 None:intergenic 40.0%
!! GGACTTATGGAAAGGTATTG+GGG - chr4.2:83137485-83137504 None:intergenic 40.0%
ACTGCTGATGCAAACTGGTT+TGG - chr4.2:83136628-83136647 None:intergenic 45.0%
AGGTTAAGGTGTACTTGAGG+AGG - chr4.2:83136546-83136565 None:intergenic 45.0%
ATAATCTGTGCAGAAGCCAG+TGG + chr4.2:83136718-83136737 MS.gene99478:CDS 45.0%
ATTTGTCAGAGGCTCATGAG+AGG - chr4.2:83136566-83136585 None:intergenic 45.0%
CTTCAAGATCAACAAGGGGA+AGG - chr4.2:83137260-83137279 None:intergenic 45.0%
CTTTGACTGCTGATGCAAAC+TGG - chr4.2:83136633-83136652 None:intergenic 45.0%
GACACCAGGATTGAGTAACA+TGG - chr4.2:83136893-83136912 None:intergenic 45.0%
GAGAGGTTAAGGTGTACTTG+AGG - chr4.2:83136549-83136568 None:intergenic 45.0%
GATGGGATTTGTGAAGAGAC+CGG + chr4.2:83137155-83137174 MS.gene99478:CDS 45.0%
GGAGTGATTTCTAAGTCGCA+TGG + chr4.2:83137063-83137082 MS.gene99478:CDS 45.0%
TCTTGGGTCCATTTCAGTCA+AGG - chr4.2:83137109-83137128 None:intergenic 45.0%
TTGATGTCAAGGAAGCTGCA+AGG - chr4.2:83137787-83137806 None:intergenic 45.0%
! GTACAAGAGTGTAGAGAGCT+TGG + chr4.2:83136840-83136859 MS.gene99478:CDS 45.0%
!! GATAAGCATTGGTGAAGCTG+AGG + chr4.2:83137287-83137306 MS.gene99478:CDS 45.0%
!! TAAAGAATTAATAAAATTAT+TGG - chr4.2:83137506-83137525 None:intergenic 5.0%
ATGTCAAGGAAGCTGCAAGG+AGG - chr4.2:83137784-83137803 None:intergenic 50.0%
CAGAGGCTCATGAGAGGTTA+AGG - chr4.2:83136560-83136579 None:intergenic 50.0%
CGCTCCATGTTACTCAATCC+TGG + chr4.2:83136886-83136905 MS.gene99478:CDS 50.0%
CTTCATAGACCACATGGGAG+GGG - chr4.2:83137392-83137411 None:intergenic 50.0%
GCTTCATAGACCACATGGGA+GGG - chr4.2:83137393-83137412 None:intergenic 50.0%
TAGAGAGCTTGGACTCTTCG+TGG + chr4.2:83136851-83136870 MS.gene99478:CDS 50.0%
TGCTTCATAGACCACATGGG+AGG - chr4.2:83137394-83137413 None:intergenic 50.0%
TTCATAGACCACATGGGAGG+GGG - chr4.2:83137391-83137410 None:intergenic 50.0%
!! CTGATGCAAACTGGTTTGGC+GGG - chr4.2:83136624-83136643 None:intergenic 50.0%
!! GCTGATGCAAACTGGTTTGG+CGG - chr4.2:83136625-83136644 None:intergenic 50.0%
!! GTGAGTGGTGGATGTACATG+AGG - chr4.2:83136955-83136974 None:intergenic 50.0%
!! TGGCGGGTTGTCTTTGTTCT+TGG - chr4.2:83136608-83136627 None:intergenic 50.0%
ACATGGGAGGGGGATCAAAG+AGG - chr4.2:83137381-83137400 None:intergenic 55.0%
CATGAGGAATGTTGAGGGGC+TGG - chr4.2:83136939-83136958 None:intergenic 55.0%
TCAAAGTGTGGTGCGACACC+AGG - chr4.2:83136907-83136926 None:intergenic 55.0%
TCTTTGATCCCCCTCCCATG+TGG + chr4.2:83137380-83137399 MS.gene99478:intron 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - chr4.2:83136934-83136953 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83136535 83137934 83136535 ID=MS.gene99478
chr4.2 mRNA 83136535 83137934 83136535 ID=MS.gene99478.t1;Parent=MS.gene99478
chr4.2 exon 83136535 83137308 83136535 ID=MS.gene99478.t1.exon1;Parent=MS.gene99478.t1
chr4.2 CDS 83136535 83137308 83136535 ID=cds.MS.gene99478.t1;Parent=MS.gene99478.t1
chr4.2 exon 83137773 83137934 83137773 ID=MS.gene99478.t1.exon2;Parent=MS.gene99478.t1
chr4.2 CDS 83137773 83137934 83137773 ID=cds.MS.gene99478.t1;Parent=MS.gene99478.t1
Gene Sequence

>MS.gene99478

ATGCTGAACCTCCTCAAGTACACCTTAACCTCTCATGAGCCTCTGACAAATACAATTTTGAAAAGCAGCTCCAAGAACAAAGACAACCCGCCAAACCAGTTTGCATCAGCAGTCAAAGTAAAAACTTGCACCCGAGACATTAAAATGGACGTTAAGCTAGTGCGAAGTAAATCTCAGAAAAAGATAATCTGTGCAGAAGCCAGTGGAAACTTAGTTGACTTCATGTTTAGCTTCCTTACAATACCATTAGGGTCCATTGTAAAGGTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGAGTGTAGAGAGCTTGGACTCTTCGTGGTGTACAAACTCGCGCTCCATGTTACTCAATCCTGGTGTCGCACCACACTTTGATTGTCCAAACCAGCCCCTCAACATTCCTCATGTACATCCACCACTCACTACATATTATTATGGTACAGGAACACCAAAGCAAGAGTATAATTATAGTTATCATGGTTATCAAACGGTAGAATTAAAGATAGAGGGAGGAGTGATTTCTAAGTCGCATGGATTGATTTATAAAGCAAAAGCCTTGACTGAAATGGACCCAAGATCCCCCAACAAATTAAAAAAAGATGTGATGGGATTTGTGAAGAGACCGGCATTATATAGTGTAGGAAATGATCTAAAAGTAAAACCACTTTCTGCTAATTCTTGCCTTTCATATTTGAAAGAATTAAACCTTCCCCTTGTTGATCTTGAAGTGAAGGTGATAAGCATTGGTGAAGCTGAGGTAAAGTGACTACTATTACTAAATTTATAATTTTTTTTCCTTTTGTTTATTTGTTGTCTAAGTTTTTAGCCTCTTTGATCCCCCTCCCATGTGGTCTATGAAGCATAGATACTTCAAAATTAGAGAAGTATTGATGTATGACACGTATCCGATACTGATACGCATCTGATACCCCCCAATACCTTTCCATAAGTCCAATAATTTTATTAATTCTTTATGAAAATATTTTCATTGATACTTCTCAAATACATGTTATGATTGGGTGGGGGAATTATATATGTAACTATGCTTACATGTTATGATTACAAGTACTTTTTTGTAAATTGCGATTACAATAATATCTTTATAAACGTTTTCGAATGCTATACAGATCTTGGTTTTGTCGTATCCTCATATCTGTGTCATGTTGAATTCATATCATACTGTGTATCCAGGATTCTTACCTCTTGATTTGCAATTTGCAGGCTTTGAGCCTCCTTGCAGCTTCCTTGACATCAAAATTTACACTGACTAGTGGACTGGAAGAATTTTTTAATGAGCAAAAACAAGATTCAAATTTGACCTCAAAGTGTGTTCAAACTAGTAGGCTTGATAAAGTTGCAAAGAAGCCTAAACTAGAAGACTGA
Protein sequence

>MS.gene99478.t1

MLNLLKYTLTSHEPLTNTILKSSSKNKDNPPNQFASAVKVKTCTRDIKMDVKLVRSKSQKKIICAEASGNLVDFMFSFLTIPLGSIVKVLDGNSFVGCVDNLYKSVESLDSSWCTNSRSMLLNPGVAPHFDCPNQPLNIPHVHPPLTTYYYGTGTPKQEYNYSYHGYQTVELKIEGGVISKSHGLIYKAKALTEMDPRSPNKLKKDVMGFVKRPALYSVGNDLKVKPLSANSCLSYLKELNLPLVDLEVKVISIGEAEALSLLAASLTSKFTLTSGLEEFFNEQKQDSNLTSKCVQTSRLDKVAKKPKLED