AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50820

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50820.t1 MTR_4g017610 66.883 462 149 3 2 462 3 461 0.0 596
MS.gene50820.t1 MTR_4g017410 65.539 473 122 8 10 458 7 462 0.0 584
MS.gene50820.t1 MTR_4g017440 65.319 470 141 5 10 458 12 480 0.0 577
MS.gene50820.t1 MTR_4g017830 61.284 483 158 9 1 458 1 479 0.0 558
MS.gene50820.t1 MTR_4g017550 65.198 454 132 10 26 458 3 451 0.0 548
MS.gene50820.t1 MTR_4g019340 60.924 476 141 11 9 458 35 491 0.0 531
MS.gene50820.t1 MTR_4g017470 57.819 486 139 9 1 458 1 448 5.19e-173 495
MS.gene50820.t1 MTR_4g019310 40.598 468 247 13 13 458 9 467 5.87e-100 308
MS.gene50820.t1 MTR_4g019310 34.343 99 51 2 269 354 8 105 2.09e-11 66.2
MS.gene50820.t1 MTR_7g066350 34.698 464 237 12 13 457 10 426 1.76e-71 233
MS.gene50820.t1 MTR_7g066350 29.268 205 119 6 265 451 5 201 2.35e-13 72.4
MS.gene50820.t1 MTR_7g066570 36.730 471 238 19 13 454 15 454 1.20e-70 234
MS.gene50820.t1 MTR_7g066340 34.549 466 236 16 13 457 13 430 2.56e-68 226
MS.gene50820.t1 MTR_7g066340 28.922 204 117 7 264 448 7 201 2.91e-14 75.1
MS.gene50820.t1 MTR_7g066340 25.610 246 139 7 10 252 261 465 2.95e-11 65.9
MS.gene50820.t1 MTR_7g058710 31.687 486 247 16 13 457 13 454 4.14e-63 212
MS.gene50820.t1 MTR_7g066260 34.110 472 230 18 13 454 16 436 7.05e-63 212
MS.gene50820.t1 MTR_7g066260 29.245 212 121 6 269 458 15 219 6.15e-13 71.2
MS.gene50820.t1 MTR_1g041315 33.619 467 251 14 13 457 15 444 1.24e-62 211
MS.gene50820.t1 MTR_7g012680 34.056 461 229 16 13 454 15 419 8.70e-61 205
MS.gene50820.t1 MTR_7g012680 26.728 217 117 6 261 440 6 217 1.05e-11 67.0
MS.gene50820.t1 MTR_7g066410 31.683 505 275 16 13 463 11 499 1.17e-58 201
MS.gene50820.t1 MTR_7g066360 35.463 454 236 15 13 454 15 423 7.81e-57 196
MS.gene50820.t1 MTR_7g066360 27.064 218 133 6 260 458 5 215 2.13e-11 66.2
MS.gene50820.t1 MTR_7g063900 32.558 473 265 17 11 454 9 456 9.57e-57 196
MS.gene50820.t1 MTR_7g066580 32.008 478 247 15 18 463 1 432 1.36e-54 189
MS.gene50820.t1 MTR_7g066430 30.818 477 287 12 9 457 1 462 5.43e-54 188
MS.gene50820.t1 MTR_7g063890 31.210 471 269 16 13 454 28 472 6.14e-52 183
MS.gene50820.t1 MTR_7g063890 30.303 198 107 6 264 441 22 208 6.93e-12 67.8
MS.gene50820.t1 MTR_7g063660 31.263 467 265 17 13 454 10 445 7.38e-52 182
MS.gene50820.t1 MTR_7g063660 32.022 178 100 6 268 431 8 178 1.81e-14 75.9
MS.gene50820.t1 MTR_7g066290 32.450 453 234 13 13 454 16 407 1.20e-51 181
MS.gene50820.t1 MTR_7g066400 31.317 463 233 17 13 454 13 411 6.24e-51 179
MS.gene50820.t1 MTR_7g062280 30.579 484 281 16 1 454 1 459 2.13e-50 179
MS.gene50820.t1 MTR_7g063840 30.297 472 270 17 5 454 7 441 2.15e-48 173
MS.gene50820.t1 MTR_7g063880 30.603 464 261 16 13 454 12 436 2.57e-48 172
MS.gene50820.t1 MTR_7g063880 28.177 181 98 6 268 428 10 178 6.89e-11 64.7
MS.gene50820.t1 MTR_7g066480 31.102 463 230 13 13 462 17 403 2.22e-47 169
MS.gene50820.t1 MTR_7g062300 29.508 488 275 17 1 457 1 450 7.60e-47 169
MS.gene50820.t1 MTR_7g058700 29.095 464 207 12 22 457 529 898 1.28e-45 170
MS.gene50820.t1 MTR_7g063830 31.798 456 235 18 13 437 11 421 9.66e-44 159
MS.gene50820.t1 MTR_7g066310 31.545 466 185 16 13 457 8 360 1.17e-42 155
MS.gene50820.t1 MTR_2g029400 29.487 468 211 13 13 454 15 389 1.26e-42 155
MS.gene50820.t1 MTR_7g063820 32.365 482 246 20 13 454 11 452 5.07e-42 155
MS.gene50820.t1 MTR_4g019240 61.789 123 44 2 1 122 1 121 1.04e-41 147
MS.gene50820.t1 MTR_3g065200 29.474 475 245 17 13 465 4 410 1.54e-41 154
MS.gene50820.t1 MTR_7g066490 28.603 451 243 9 13 462 17 389 1.03e-39 148
MS.gene50820.t1 MTR_7g066490 29.032 186 110 6 254 426 1 177 5.63e-11 64.7
MS.gene50820.t1 MTR_7g063670 31.489 470 250 20 3 454 10 425 6.49e-39 147
MS.gene50820.t1 MTR_3g007740 29.892 465 259 18 9 457 6 419 1.03e-38 146
MS.gene50820.t1 MTR_7g063920 28.035 453 255 12 13 454 12 404 1.21e-38 145
MS.gene50820.t1 MTR_1g014060 28.571 455 199 18 21 454 9 358 3.14e-33 129
MS.gene50820.t1 MTR_7g066680 37.264 212 101 7 268 458 85 285 6.00e-26 108
MS.gene50820.t1 MTR_7g066280 26.733 404 214 12 99 457 1 367 3.44e-25 107
MS.gene50820.t1 MTR_7g062270 35.000 200 98 10 13 200 11 190 2.17e-19 90.1
MS.gene50820.t1 MTR_8g445860 27.755 245 143 7 10 240 11 235 9.88e-17 79.7
MS.gene50820.t1 MTR_8g445860 26.923 208 122 5 269 454 13 212 6.30e-15 74.7
MS.gene50820.t1 MTR_7g063720 30.000 200 104 5 269 454 23 200 1.10e-16 79.7
MS.gene50820.t1 MTR_8g023240 23.782 349 221 11 12 345 37 355 2.51e-16 82.0
MS.gene50820.t1 MTR_1g115440 27.197 239 149 7 13 240 12 236 5.60e-15 74.7
MS.gene50820.t1 MTR_4g019330 30.636 173 70 4 283 454 32 155 8.40e-14 70.1
MS.gene50820.t1 MTR_6g006800 62.903 62 12 2 1 62 1 51 1.84e-13 65.5
MS.gene50820.t1 MTR_0150s0010 28.750 240 144 8 13 240 11 235 5.69e-13 68.9
MS.gene50820.t1 MTR_7g100420 28.627 255 145 10 220 454 134 371 9.10e-13 70.1
MS.gene50820.t1 MTR_7g100420 67.442 43 14 0 12 54 39 81 2.08e-11 65.9
MS.gene50820.t1 MTR_1g115455 35.252 139 71 5 13 145 10 135 5.45e-12 65.9
MS.gene50820.t1 MTR_1g115435 27.602 221 120 8 10 225 5 190 1.24e-11 64.7
MS.gene50820.t1 MTR_1g115450 37.975 79 41 1 269 339 7 85 1.87e-11 62.4
MS.gene50820.t1 MTR_0150s0030 32.000 100 53 2 269 359 8 101 7.60e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50820.t1 AT5G01120 34.105 475 267 16 13 458 10 467 8.76e-66 220
MS.gene50820.t1 AT5G43240 33.333 486 260 19 13 458 10 471 3.36e-57 197
MS.gene50820.t1 AT5G43240 33.333 486 260 19 13 458 10 471 3.36e-57 197
MS.gene50820.t1 AT5G43240 33.333 486 260 19 13 458 10 471 3.36e-57 197
MS.gene50820.t1 AT5G01130 33.186 452 255 16 13 457 10 421 4.32e-55 190
MS.gene50820.t1 AT3G09140 30.328 488 278 18 10 458 7 471 2.44e-51 181
MS.gene50820.t1 AT5G01150 32.334 467 272 13 15 458 12 457 1.38e-50 179
MS.gene50820.t1 AT3G09110 35.061 328 180 11 15 330 12 318 6.13e-48 168
MS.gene50820.t1 AT3G09140 28.682 516 278 19 10 458 7 499 3.84e-46 168
MS.gene50820.t1 AT5G01140 31.072 457 255 15 15 454 12 425 9.55e-46 166
MS.gene50820.t1 AT3G09120 32.934 334 180 11 10 323 4 313 4.06e-43 154
MS.gene50820.t1 AT5G37320 29.284 461 284 17 19 458 18 457 6.78e-36 139
MS.gene50820.t1 AT5G43240 30.422 332 185 11 160 458 9 327 5.46e-28 114

Find 92 sgRNAs with CRISPR-Local

Find 147 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTATTACAAATACAATTT+TGG 0.077846 4.1:+80020090 MS.gene50820:CDS
CATCGTCTGAGTTGAGATTT+TGG 0.163224 4.1:-80019103 None:intergenic
CCCAAAATTGTATTTGTAAT+AGG 0.221679 4.1:-80020091 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.255481 4.1:-80019305 None:intergenic
TAATAGACAACCTAAGAATT+TGG 0.261898 4.1:+80019307 MS.gene50820:CDS
GTGGGTTGTGGTATTAAATA+AGG 0.297283 4.1:-80020454 None:intergenic
CTCTAACTACTGATGCAAAT+TGG 0.299041 4.1:-80020147 None:intergenic
AGCTTCCTTACCATGCCATT+GGG 0.310690 4.1:+80020283 MS.gene50820:CDS
TGTTGTCAGTGGTGGCTAAA+AGG 0.330255 4.1:-80019013 None:intergenic
ATCTCAACTCAGACGATGTT+TGG 0.331962 4.1:+80019108 MS.gene50820:CDS
CTTAGAGATGTTGGTTCCAT+TGG 0.338874 4.1:+80019439 MS.gene50820:CDS
CCACAACCCACGTATTATTA+TGG 0.345905 4.1:+80020466 MS.gene50820:CDS
GTGAAGAGAGCAACATTATA+TGG 0.349402 4.1:+80020619 MS.gene50820:CDS
CGGCCCGTATCCAAATTCTT+AGG 0.353895 4.1:-80019317 None:intergenic
AGGCAAAACTTAGCAGAAAC+TGG 0.372974 4.1:-80020667 None:intergenic
AATTCTTCACAAGGATTCCT+TGG 0.390278 4.1:-80019164 None:intergenic
GGAATGTTGAGGGGCTGGTT+TGG 0.392660 4.1:-80020433 None:intergenic
TAGCTTCCTTACCATGCCAT+TGG 0.394737 4.1:+80020282 MS.gene50820:CDS
AATCCCACAGCGCCTTCTCT+TGG 0.395694 4.1:-80020595 None:intergenic
CAATGGAACCAACATCTCTA+AGG 0.402119 4.1:-80019438 None:intergenic
GTACAAGAGTGTTCAGAACT+TGG 0.408992 4.1:+80020360 MS.gene50820:CDS
ACTGATGCAAATTGGTTAGC+TGG 0.412013 4.1:-80020139 None:intergenic
TTAGAGATGTTGGTTCCATT+GGG 0.416438 4.1:+80019440 MS.gene50820:CDS
AATAAGGAATGTTGAGGGGC+TGG 0.425217 4.1:-80020438 None:intergenic
AATTCCCCTCAAAATCTTAG+TGG 0.429720 4.1:+80018893 MS.gene50820:CDS
AAGAATTTGGATACGGGCCG+GGG 0.431114 4.1:+80019320 MS.gene50820:CDS
CATTGGTGAATTTGAGGTAA+AGG 0.432182 4.1:+80020747 MS.gene50820:CDS
ATCCCACAGCGCCTTCTCTT+GGG 0.434305 4.1:-80020594 None:intergenic
TAAGAATTTGGATACGGGCC+GGG 0.434440 4.1:+80019319 MS.gene50820:CDS
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.1:+80018978 MS.gene50820:CDS
GTTGAGTTCCTTAGAGATGT+TGG 0.445404 4.1:+80019430 MS.gene50820:CDS
GGATTAAGTAACACAGAAGT+AGG 0.447147 4.1:-80020385 None:intergenic
ACTTCTGTGTTACTTAATCC+TGG 0.451739 4.1:+80020388 MS.gene50820:CDS
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.1:-80018983 None:intergenic
GATAAGCATTGGTGAATTTG+AGG 0.462994 4.1:+80020741 MS.gene50820:CDS
CAAAACCTTGATTGCACCTG+TGG 0.476027 4.1:+80019275 MS.gene50820:CDS
CTGGTATAGCACCACAACTT+AGG 0.481431 4.1:+80020407 MS.gene50820:CDS
GACTATTATCCTACAAAAGA+AGG 0.481572 4.1:+80020514 MS.gene50820:CDS
TTCAAGGTCGTTGATTGCAC+TGG 0.491294 4.1:+80020570 MS.gene50820:CDS
AAGGAACTCAACATGGAACT+TGG 0.499089 4.1:-80019419 None:intergenic
AACAAATGTTGCTGCGTCCA+AGG 0.504062 4.1:+80019147 MS.gene50820:CDS
GGAGTCTTCGTTAGAGAAAG+TGG 0.504992 4.1:+80019359 MS.gene50820:CDS
CCATAATAATACGTGGGTTG+TGG 0.507333 4.1:-80020466 None:intergenic
CTAAGTTGTGGTGCTATACC+AGG 0.515459 4.1:-80020406 None:intergenic
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4.1:-80019302 None:intergenic
CATCTCTAAGGAACTCAACA+TGG 0.531180 4.1:-80019426 None:intergenic
ACACATCCAGCAAAAGAGTC+AGG 0.540251 4.1:-80020328 None:intergenic
ATGACTCTGAGAGTTGTTGT+AGG 0.542216 4.1:-80019040 None:intergenic
TGGTAAAATCTTCAAATCAT+CGG 0.545386 4.1:-80019399 None:intergenic
AGAGATATTTGATGTTGTCA+AGG 0.547142 4.1:-80019070 None:intergenic
CTGATGCAAATTGGTTAGCT+GGG 0.547375 4.1:-80020138 None:intergenic
CTGTCGACCATAATAATACG+TGG 0.548759 4.1:-80020473 None:intergenic
ACAACCTAAGAATTTGGATA+CGG 0.549258 4.1:+80019313 MS.gene50820:CDS
TCTGTTCTTTCAGATATGAA+AGG 0.549535 4.1:-80020687 None:intergenic
TTGTAGGTCATTGTTGTCAG+TGG 0.554864 4.1:-80019024 None:intergenic
TATTAAATAAGGAATGTTGA+GGG 0.558962 4.1:-80020443 None:intergenic
TCTTGACGATCTTGAAGTGA+AGG 0.559099 4.1:+80020717 MS.gene50820:CDS
TAGGTCATTGTTGTCAGTGG+TGG 0.559820 4.1:-80019021 None:intergenic
CTAAGAATTTGGATACGGGC+CGG 0.563009 4.1:+80019318 MS.gene50820:CDS
CACTTGAGGCATAATTCCCC+CGG 0.572600 4.1:-80019337 None:intergenic
TGGACCCCAATGGCATGGTA+AGG 0.574831 4.1:-80020288 None:intergenic
TACAATGGACCCCAATGGCA+TGG 0.580817 4.1:-80020293 None:intergenic
GCTTCCTTACCATGCCATTG+GGG 0.583133 4.1:+80020284 MS.gene50820:CDS
GACCCAAGAGAAGGCGCTGT+GGG 0.589843 4.1:+80020592 MS.gene50820:CDS
TTCATGCATAATTCTTCACA+AGG 0.597426 4.1:-80019173 None:intergenic
GTATTAAATAAGGAATGTTG+AGG 0.602215 4.1:-80020444 None:intergenic
TATTTGATGTTGTCAAGGAA+TGG 0.603436 4.1:-80019065 None:intergenic
AGAATTTGGATACGGGCCGG+GGG 0.604267 4.1:+80019321 MS.gene50820:CDS
CAACCTAAGAATTTGGATAC+GGG 0.615212 4.1:+80019314 MS.gene50820:CDS
TGGTTTGGACACCTAAGTTG+TGG 0.615467 4.1:-80020418 None:intergenic
GATTAAGTAACACAGAAGTA+GGG 0.615768 4.1:-80020384 None:intergenic
TTTCTGATATCATCGGGACA+AGG 0.617619 4.1:-80020175 None:intergenic
GAAGAAGTCACACACAACAT+TGG 0.621785 4.1:+80019485 MS.gene50820:CDS
ATTGCACTGGACCCAAGAGA+AGG 0.623682 4.1:+80020583 MS.gene50820:CDS
GTCAAGTCTGCATAACCCAA+TGG 0.629435 4.1:-80019455 None:intergenic
ATTAAATAAGGAATGTTGAG+GGG 0.631161 4.1:-80020442 None:intergenic
AGAGCAACATTATATGGTGT+AGG 0.631695 4.1:+80020625 MS.gene50820:CDS
ATAATCTATGCAGAAGCGAA+CGG 0.635454 4.1:+80020238 MS.gene50820:CDS
ACACAACATTGGCAAGCAAG+AGG 0.636713 4.1:+80019496 MS.gene50820:CDS
AGGTCCTAAAAGCTTTACAA+TGG 0.639517 4.1:-80020308 None:intergenic
TTCACTTCAAGATCGTCAAG+AGG 0.643323 4.1:-80020715 None:intergenic
TCTAACGAAGACTCCACTTG+AGG 0.644921 4.1:-80019351 None:intergenic
AGGCGAAGATATACTTGAGG+AGG 0.647256 4.1:-80020060 None:intergenic
GAGAGGCGAAGATATACTTG+AGG 0.649287 4.1:-80020063 None:intergenic
GAAGTGAAGGTGATAAGCAT+TGG 0.654807 4.1:+80020730 MS.gene50820:CDS
ATTTGTAATAGGCTCATGAG+AGG 0.655380 4.1:-80020080 None:intergenic
AGCTTTACAATGGACCCCAA+TGG 0.659874 4.1:-80020298 None:intergenic
GGACCCAAGAGAAGGCGCTG+TGG 0.662998 4.1:+80020591 MS.gene50820:CDS
GGTGGCTAAAAGGCGAACGA+TGG 0.684330 4.1:-80019003 None:intergenic
TGTCGACCATAATAATACGT+GGG 0.685398 4.1:-80020472 None:intergenic
GTTGTTGAATGTTGATGACA+CGG 0.700176 4.1:+80019199 MS.gene50820:CDS
AGGCGAACGATGGTACCAAG+TGG 0.729828 4.1:-80018993 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAGAATATATTAAAAA+AGG + chr4.1:80019803-80019822 MS.gene50820:intron 10.0%
!!! TTTTTAATAGTATTTTACCT+AGG + chr4.1:80019944-80019963 MS.gene50820:intron 15.0%
!! ATTGTAAGAATAACAGTTTA+AGG - chr4.1:80020009-80020028 None:intergenic 20.0%
!! CTATACAAAATGTTAAAACA+TGG - chr4.1:80020032-80020051 None:intergenic 20.0%
!! TATTAAATAAGGAATGTTGA+GGG - chr4.1:80020446-80020465 None:intergenic 20.0%
!!! ATGAACTAATTTTAAGTTCA+AGG + chr4.1:80020554-80020573 MS.gene50820:CDS 20.0%
!!! CCTATTACAAATACAATTTT+GGG + chr4.1:80020091-80020110 MS.gene50820:CDS 20.0%
!!! TATCTCTAAAAACATTATTG+CGG + chr4.1:80019659-80019678 MS.gene50820:intron 20.0%
! AATAAGAGAAGAAACATGTT+AGG + chr4.1:80019849-80019868 MS.gene50820:intron 25.0%
! ATCTTTATTGTGGATAAATG+CGG + chr4.1:80019612-80019631 MS.gene50820:intron 25.0%
! ATTAAATAAGGAATGTTGAG+GGG - chr4.1:80020445-80020464 None:intergenic 25.0%
! CCCAAAATTGTATTTGTAAT+AGG - chr4.1:80020094-80020113 None:intergenic 25.0%
! GTAAGAAAAAGTCAATGTAA+TGG - chr4.1:80019527-80019546 None:intergenic 25.0%
! GTATTAAATAAGGAATGTTG+AGG - chr4.1:80020447-80020466 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG + chr4.1:80019307-80019326 MS.gene50820:CDS 25.0%
! TATGTTGTGAATCTTTATTG+TGG + chr4.1:80019602-80019621 MS.gene50820:intron 25.0%
! TGGTAAAATCTTCAAATCAT+CGG - chr4.1:80019402-80019421 None:intergenic 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG - chr4.1:80019308-80019327 None:intergenic 25.0%
!! ATGCTAGCCAAAAAAATTTT+TGG - chr4.1:80019973-80019992 None:intergenic 25.0%
!! GCCTATTACAAATACAATTT+TGG + chr4.1:80020090-80020109 MS.gene50820:CDS 25.0%
!! TGCTAGCCAAAAAAATTTTT+GGG - chr4.1:80019972-80019991 None:intergenic 25.0%
!!! AAAAAAATTTTTGGGAACCT+AGG - chr4.1:80019964-80019983 None:intergenic 25.0%
!!! ATCCATTTTTCTGATATCAT+CGG - chr4.1:80020185-80020204 None:intergenic 25.0%
!!! GTTTTTAGAGATATTCACAT+CGG - chr4.1:80019652-80019671 None:intergenic 25.0%
!!! TAGGTTCCCAAAAATTTTTT+TGG + chr4.1:80019963-80019982 MS.gene50820:intron 25.0%
!!! TGACTTTTTCTTACTTACAT+AGG + chr4.1:80019533-80019552 MS.gene50820:intron 25.0%
AAGAAACATGTTAGGATAAG+AGG + chr4.1:80019857-80019876 MS.gene50820:intron 30.0%
ACAACCTAAGAATTTGGATA+CGG + chr4.1:80019313-80019332 MS.gene50820:CDS 30.0%
AGAGATATTTGATGTTGTCA+AGG - chr4.1:80019073-80019092 None:intergenic 30.0%
ATAAGTCTGCTAGTAATATC+AGG + chr4.1:80019738-80019757 MS.gene50820:intron 30.0%
GACTATTATCCTACAAAAGA+AGG + chr4.1:80020514-80020533 MS.gene50820:CDS 30.0%
GATTAAGTAACACAGAAGTA+GGG - chr4.1:80020387-80020406 None:intergenic 30.0%
TATTTGATGTTGTCAAGGAA+TGG - chr4.1:80019068-80019087 None:intergenic 30.0%
TCATAAGTTAGCATAATTGC+AGG - chr4.1:80019766-80019785 None:intergenic 30.0%
TCTGTTCTTTCAGATATGAA+AGG - chr4.1:80020690-80020709 None:intergenic 30.0%
TCTTACTTACATAGGATCTT+AGG + chr4.1:80019541-80019560 MS.gene50820:intron 30.0%
TTCATGCATAATTCTTCACA+AGG - chr4.1:80019176-80019195 None:intergenic 30.0%
! AGAAAGTGGACAAAAGTTTT+TGG + chr4.1:80019373-80019392 MS.gene50820:CDS 30.0%
! AGTTGAGATTTTGGACAATT+TGG - chr4.1:80019097-80019116 None:intergenic 30.0%
! GAAATCAATCCTTCTTTTGT+AGG - chr4.1:80020526-80020545 None:intergenic 30.0%
! TAGGATAAGAGGTATAGTTA+GGG + chr4.1:80019868-80019887 MS.gene50820:intron 30.0%
! TTAGGATAAGAGGTATAGTT+AGG + chr4.1:80019867-80019886 MS.gene50820:intron 30.0%
! TTTGTCCACTAAGATTTTGA+GGG - chr4.1:80018901-80018920 None:intergenic 30.0%
!! TCCATTTTTCTGATATCATC+GGG - chr4.1:80020184-80020203 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG + chr4.1:80018923-80018942 MS.gene50820:CDS 30.0%
!!! ATTTTTTTGGCTAGCATTAG+TGG + chr4.1:80019976-80019995 MS.gene50820:intron 30.0%
AATTCCCCTCAAAATCTTAG+TGG + chr4.1:80018893-80018912 MS.gene50820:CDS 35.0%
AATTCTTCACAAGGATTCCT+TGG - chr4.1:80019167-80019186 None:intergenic 35.0%
AGAGCAACATTATATGGTGT+AGG + chr4.1:80020625-80020644 MS.gene50820:CDS 35.0%
AGGTCCTAAAAGCTTTACAA+TGG - chr4.1:80020311-80020330 None:intergenic 35.0%
AGTTAGGGAAGTTAAGTAGT+TGG + chr4.1:80019883-80019902 MS.gene50820:intron 35.0%
ATAACTGAAACCTCAAGTGA+GGG - chr4.1:80019585-80019604 None:intergenic 35.0%
ATAATCTATGCAGAAGCGAA+CGG + chr4.1:80020238-80020257 MS.gene50820:CDS 35.0%
ATTTGTAATAGGCTCATGAG+AGG - chr4.1:80020083-80020102 None:intergenic 35.0%
CAACCTAAGAATTTGGATAC+GGG + chr4.1:80019314-80019333 MS.gene50820:CDS 35.0%
CTCTAACTACTGATGCAAAT+TGG - chr4.1:80020150-80020169 None:intergenic 35.0%
GGATTAAGTAACACAGAAGT+AGG - chr4.1:80020388-80020407 None:intergenic 35.0%
GTGAAGAGAGCAACATTATA+TGG + chr4.1:80020619-80020638 MS.gene50820:CDS 35.0%
TAGCAGACTTATATCCCAAA+GGG - chr4.1:80019730-80019749 None:intergenic 35.0%
TCCCGATGATATCAGAAAAA+TGG + chr4.1:80020180-80020199 MS.gene50820:CDS 35.0%
TGTCGACCATAATAATACGT+GGG - chr4.1:80020475-80020494 None:intergenic 35.0%
! CATTGGTGAATTTGAGGTAA+AGG + chr4.1:80020747-80020766 MS.gene50820:CDS 35.0%
! GTGGGTTGTGGTATTAAATA+AGG - chr4.1:80020457-80020476 None:intergenic 35.0%
! GTTTGTCCACTAAGATTTTG+AGG - chr4.1:80018902-80018921 None:intergenic 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG - chr4.1:80019305-80019324 None:intergenic 35.0%
! TTGTCCACTAAGATTTTGAG+GGG - chr4.1:80018900-80018919 None:intergenic 35.0%
!! ACTTCTGTGTTACTTAATCC+TGG + chr4.1:80020388-80020407 MS.gene50820:CDS 35.0%
!! GATAAGCATTGGTGAATTTG+AGG + chr4.1:80020741-80020760 MS.gene50820:CDS 35.0%
!! GTTGTTGAATGTTGATGACA+CGG + chr4.1:80019199-80019218 MS.gene50820:CDS 35.0%
!! TTAGAGATGTTGGTTCCATT+GGG + chr4.1:80019440-80019459 MS.gene50820:CDS 35.0%
AAGGAACTCAACATGGAACT+TGG - chr4.1:80019422-80019441 None:intergenic 40.0%
ACTGATGCAAATTGGTTAGC+TGG - chr4.1:80020142-80020161 None:intergenic 40.0%
AGAACTATGCTTCACCCTTT+GGG + chr4.1:80019713-80019732 MS.gene50820:intron 40.0%
AGGCAAAACTTAGCAGAAAC+TGG - chr4.1:80020670-80020689 None:intergenic 40.0%
ATCTCAACTCAGACGATGTT+TGG + chr4.1:80019108-80019127 MS.gene50820:CDS 40.0%
CAATGGAACCAACATCTCTA+AGG - chr4.1:80019441-80019460 None:intergenic 40.0%
CATAACTGAAACCTCAAGTG+AGG - chr4.1:80019586-80019605 None:intergenic 40.0%
CCACAACCCACGTATTATTA+TGG + chr4.1:80020466-80020485 MS.gene50820:CDS 40.0%
CTAGCAGACTTATATCCCAA+AGG - chr4.1:80019731-80019750 None:intergenic 40.0%
CTGTCGACCATAATAATACG+TGG - chr4.1:80020476-80020495 None:intergenic 40.0%
GAAGAAGTCACACACAACAT+TGG + chr4.1:80019485-80019504 MS.gene50820:CDS 40.0%
GAAGTGAAGGTGATAAGCAT+TGG + chr4.1:80020730-80020749 MS.gene50820:CDS 40.0%
GAGGGTTCATGGTTTAACAA+TGG - chr4.1:80019567-80019586 None:intergenic 40.0%
GTTGAGTTCCTTAGAGATGT+TGG + chr4.1:80019430-80019449 MS.gene50820:CDS 40.0%
TAGAACTATGCTTCACCCTT+TGG + chr4.1:80019712-80019731 MS.gene50820:intron 40.0%
TCTTGACGATCTTGAAGTGA+AGG + chr4.1:80020717-80020736 MS.gene50820:CDS 40.0%
TTCACTTCAAGATCGTCAAG+AGG - chr4.1:80020718-80020737 None:intergenic 40.0%
TTGTAGGTCATTGTTGTCAG+TGG - chr4.1:80019027-80019046 None:intergenic 40.0%
TTTCTGATATCATCGGGACA+AGG - chr4.1:80020178-80020197 None:intergenic 40.0%
! ATGACTCTGAGAGTTGTTGT+AGG - chr4.1:80019043-80019062 None:intergenic 40.0%
! CATCTCTAAGGAACTCAACA+TGG - chr4.1:80019429-80019448 None:intergenic 40.0%
! CCATAATAATACGTGGGTTG+TGG - chr4.1:80020469-80020488 None:intergenic 40.0%
! CTGATGCAAATTGGTTAGCT+GGG - chr4.1:80020141-80020160 None:intergenic 40.0%
! GGGTCCATTGTAAAGCTTTT+AGG + chr4.1:80020304-80020323 MS.gene50820:CDS 40.0%
! TCTATTAGGAGGTTTTCCAC+AGG - chr4.1:80019294-80019313 None:intergenic 40.0%
!! CATCGTCTGAGTTGAGATTT+TGG - chr4.1:80019106-80019125 None:intergenic 40.0%
!! CTTAGAGATGTTGGTTCCAT+TGG + chr4.1:80019439-80019458 MS.gene50820:CDS 40.0%
!! GTACAAGAGTGTTCAGAACT+TGG + chr4.1:80020360-80020379 MS.gene50820:CDS 40.0%
!! TTTTGGCTAGCATTAGTGGT+GGG + chr4.1:80019980-80019999 MS.gene50820:intron 40.0%
!!! TTTTTGGCTAGCATTAGTGG+TGG + chr4.1:80019979-80019998 MS.gene50820:intron 40.0%
AAACCATGAACCCTCACTTG+AGG + chr4.1:80019572-80019591 MS.gene50820:intron 45.0%
AAACCTCAAGTGAGGGTTCA+TGG - chr4.1:80019578-80019597 None:intergenic 45.0%
AACAAATGTTGCTGCGTCCA+AGG + chr4.1:80019147-80019166 MS.gene50820:CDS 45.0%
AATAAGGAATGTTGAGGGGC+TGG - chr4.1:80020441-80020460 None:intergenic 45.0%
ACACAACATTGGCAAGCAAG+AGG + chr4.1:80019496-80019515 MS.gene50820:CDS 45.0%
ACACATCCAGCAAAAGAGTC+AGG - chr4.1:80020331-80020350 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG + chr4.1:80018978-80018997 MS.gene50820:CDS 45.0%
AGCTTCCTTACCATGCCATT+GGG + chr4.1:80020283-80020302 MS.gene50820:CDS 45.0%
AGCTTTACAATGGACCCCAA+TGG - chr4.1:80020301-80020320 None:intergenic 45.0%
AGGCGAAGATATACTTGAGG+AGG - chr4.1:80020063-80020082 None:intergenic 45.0%
CAAAACCTTGATTGCACCTG+TGG + chr4.1:80019275-80019294 MS.gene50820:CDS 45.0%
CTAAGAATTTGGATACGGGC+CGG + chr4.1:80019318-80019337 MS.gene50820:CDS 45.0%
CTGGTATAGCACCACAACTT+AGG + chr4.1:80020407-80020426 MS.gene50820:CDS 45.0%
GAGAGGCGAAGATATACTTG+AGG - chr4.1:80020066-80020085 None:intergenic 45.0%
GGAGTCTTCGTTAGAGAAAG+TGG + chr4.1:80019359-80019378 MS.gene50820:CDS 45.0%
GTCAAGTCTGCATAACCCAA+TGG - chr4.1:80019458-80019477 None:intergenic 45.0%
TAAGAATTTGGATACGGGCC+GGG + chr4.1:80019319-80019338 MS.gene50820:CDS 45.0%
TAGCTTCCTTACCATGCCAT+TGG + chr4.1:80020282-80020301 MS.gene50820:CDS 45.0%
TAGGTCATTGTTGTCAGTGG+TGG - chr4.1:80019024-80019043 None:intergenic 45.0%
TCTAACGAAGACTCCACTTG+AGG - chr4.1:80019354-80019373 None:intergenic 45.0%
TGGTACCAAGTGGCAAAGAT+AGG - chr4.1:80018986-80019005 None:intergenic 45.0%
TGTTGTCAGTGGTGGCTAAA+AGG - chr4.1:80019016-80019035 None:intergenic 45.0%
! GACTCTTTTGCTGGATGTGT+TGG + chr4.1:80020331-80020350 MS.gene50820:CDS 45.0%
! GTTTTCCACAGGTGCAATCA+AGG - chr4.1:80019283-80019302 None:intergenic 45.0%
! TGGTTTGGACACCTAAGTTG+TGG - chr4.1:80020421-80020440 None:intergenic 45.0%
! TTAGGACCTGACTCTTTTGC+TGG + chr4.1:80020322-80020341 MS.gene50820:CDS 45.0%
! TTCAAGGTCGTTGATTGCAC+TGG + chr4.1:80020570-80020589 MS.gene50820:CDS 45.0%
!! CTAAGTTGTGGTGCTATACC+AGG - chr4.1:80020409-80020428 None:intergenic 45.0%
AAGAATTTGGATACGGGCCG+GGG + chr4.1:80019320-80019339 MS.gene50820:CDS 50.0%
ATTGCACTGGACCCAAGAGA+AGG + chr4.1:80020583-80020602 MS.gene50820:CDS 50.0%
ATTGTGGATAAATGCGGCCG+TGG + chr4.1:80019618-80019637 MS.gene50820:intron 50.0%
CACTTGAGGCATAATTCCCC+CGG - chr4.1:80019340-80019359 None:intergenic 50.0%
CGGCCCGTATCCAAATTCTT+AGG - chr4.1:80019320-80019339 None:intergenic 50.0%
GCTTCCTTACCATGCCATTG+GGG + chr4.1:80020284-80020303 MS.gene50820:CDS 50.0%
TACAATGGACCCCAATGGCA+TGG - chr4.1:80020296-80020315 None:intergenic 50.0%
TCACATCGGCATTGCAACCA+CGG - chr4.1:80019638-80019657 None:intergenic 50.0%
! TGCGGCTGCAACTGTGTTTT+TGG + chr4.1:80019677-80019696 MS.gene50820:intron 50.0%
AATCCCACAGCGCCTTCTCT+TGG - chr4.1:80020598-80020617 None:intergenic 55.0%
AGAATTTGGATACGGGCCGG+GGG + chr4.1:80019321-80019340 MS.gene50820:CDS 55.0%
AGGCGAACGATGGTACCAAG+TGG - chr4.1:80018996-80019015 None:intergenic 55.0%
ATCCCACAGCGCCTTCTCTT+GGG - chr4.1:80020597-80020616 None:intergenic 55.0%
CGGGGGAATTATGCCTCAAG+TGG + chr4.1:80019338-80019357 MS.gene50820:CDS 55.0%
GGTGGCTAAAAGGCGAACGA+TGG - chr4.1:80019006-80019025 None:intergenic 55.0%
TGGACCCCAATGGCATGGTA+AGG - chr4.1:80020291-80020310 None:intergenic 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG - chr4.1:80020436-80020455 None:intergenic 55.0%
!! GACCCAAGAGAAGGCGCTGT+GGG + chr4.1:80020592-80020611 MS.gene50820:CDS 60.0%
!! GGACCCAAGAGAAGGCGCTG+TGG + chr4.1:80020591-80020610 MS.gene50820:CDS 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80018858 80020789 80018858 ID=MS.gene50820
chr4.1 mRNA 80018858 80020789 80018858 ID=MS.gene50820.t1;Parent=MS.gene50820
chr4.1 exon 80018858 80019517 80018858 ID=MS.gene50820.t1.exon1;Parent=MS.gene50820.t1
chr4.1 CDS 80018858 80019517 80018858 ID=cds.MS.gene50820.t1;Parent=MS.gene50820.t1
chr4.1 exon 80020052 80020789 80020052 ID=MS.gene50820.t1.exon2;Parent=MS.gene50820.t1
chr4.1 CDS 80020052 80020789 80020052 ID=cds.MS.gene50820.t1;Parent=MS.gene50820.t1
Gene Sequence

>MS.gene50820

ATGGCTTATCGACGACAACAAATATTGTCAAAAACAATTCCCCTCAAAATCTTAGTGGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGATACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCCACCACTGACAACAATGACCTACAACAACTCTCAGAGTCATCACCATTCCTTGACAACATCAAATATCTCTACCAAATTGTCCAAAATCTCAACTCAGACGATGTTTGGAACAACTCTGTGTGCAAACAAATGTTGCTGCGTCCAAGGAATCCTTGTGAAGAATTATGCATGAAGCTGTTGTTGAATGTTGATGACACGGAGACATCAAGTAAATTTTATGTGTGTGATTCTTGTTACAAGTTCACTAATTTTCAAAACCTTGATTGCACCTGTGGAAAACCTCCTAATAGACAACCTAAGAATTTGGATACGGGCCGGGGGAATTATGCCTCAAGTGGAGTCTTCGTTAGAGAAAGTGGACAAAAGTTTTTGGTGTCCGATGATTTGAAGATTTTACCAAGTTCCATGTTGAGTTCCTTAGAGATGTTGGTTCCATTGGGTTATGCAGACTTGACTGAACTAGAAGAAGTCACACACAACATTGGCAAGCAAGAGGTTAGTCCATTACATTGACTTTTTCTTACTTACATAGGATCTTAGGCCATTGTTAAACCATGAACCCTCACTTGAGGTTTCAGTTATGTTGTGAATCTTTATTGTGGATAAATGCGGCCGTGGTTGCAATGCCGATGTGAATATCTCTAAAAACATTATTGCGGCTGCAACTGTGTTTTTGGATTACAATAATTTAGAACTATGCTTCACCCTTTGGGATATAAGTCTGCTAGTAATATCAGGAACCTGCAATTATGCTAACTTATGATTTTATTTTTGACAAGCTAAAGAGAATATATTAAAAAAGGTACTTCAAAGAAAATGCATACTAAATAAGAGAAGAAACATGTTAGGATAAGAGGTATAGTTAGGGAAGTTAAGTAGTTGGAGCATGAAATTATGTGTAATTAGAATCAATATTACTTATTTTTAATAGTATTTTACCTAGGTTCCCAAAAATTTTTTTGGCTAGCATTAGTGGTGGGTTTCCTTAAACTGTTATTCTTACAATCCATGTTTTAACATTTTGTATAGATATTGAACCTCCTCAAGTATATCTTCGCCTCTCATGAGCCTATTACAAATACAATTTTGGGAAGTAGCTCAAACAACAGAAACAACCCAGCTAACCAATTTGCATCAGTAGTTAGAGTAACACCTTGTCCCGATGATATCAGAAAAATGGATGTTAAAGTAATGCAAAGTAAGTCTCAGAAAAATATAATCTATGCAGAAGCGAACGGTGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTGGGGTCCATTGTAAAGCTTTTAGGACCTGACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTTCAGAACTTGGACCCTACTTCTGTGTTACTTAATCCTGGTATAGCACCACAACTTAGGTGTCCAAACCAGCCCCTCAACATTCCTTATTTAATACCACAACCCACGTATTATTATGGTCGACAGAAAAATGATGATCACTATGACTATTATCCTACAAAAGAAGGATTGATTTCTAAGTCATATGAACTAATTTTAAGTTCAAGGTCGTTGATTGCACTGGACCCAAGAGAAGGCGCTGTGGGATTTGTGAAGAGAGCAACATTATATGGTGTAGGAGATGATCTAAAAGTAAAACCAGTTTCTGCTAAGTTTTGCCTTTCATATCTGAAAGAACAGAATCTTCCTCTTGACGATCTTGAAGTGAAGGTGATAAGCATTGGTGAATTTGAGGTAAAGGTTGCTCTTATATTAGACTAA
Protein sequence

>MS.gene50820.t1

MAYRRQQILSKTIPLKILVDKQSNKVVFVEATKDFVDTLFSFLSLPLGTIVRLLATTDNNDLQQLSESSPFLDNIKYLYQIVQNLNSDDVWNNSVCKQMLLRPRNPCEELCMKLLLNVDDTETSSKFYVCDSCYKFTNFQNLDCTCGKPPNRQPKNLDTGRGNYASSGVFVRESGQKFLVSDDLKILPSSMLSSLEMLVPLGYADLTELEEVTHNIGKQEILNLLKYIFASHEPITNTILGSSSNNRNNPANQFASVVRVTPCPDDIRKMDVKVMQSKSQKNIIYAEANGDFVDFIFSFLTMPLGSIVKLLGPDSFAGCVGNLYKSVQNLDPTSVLLNPGIAPQLRCPNQPLNIPYLIPQPTYYYGRQKNDDHYDYYPTKEGLISKSYELILSSRSLIALDPREGAVGFVKRATLYGVGDDLKVKPVSAKFCLSYLKEQNLPLDDLEVKVISIGEFEVKVALILD