AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037545

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037545.t1 MTR_4g017610 89.216 306 33 0 1 306 1 306 0.0 556
MS.gene037545.t1 MTR_4g017410 67.752 307 91 4 8 306 4 310 1.24e-140 407
MS.gene037545.t1 MTR_4g019340 63.072 306 100 5 12 306 37 340 5.43e-127 372
MS.gene037545.t1 MTR_4g019340 29.091 220 130 7 16 233 306 501 3.32e-13 70.1
MS.gene037545.t1 MTR_4g017830 60.656 305 117 2 3 306 2 304 3.36e-126 370
MS.gene037545.t1 MTR_4g017830 26.459 257 129 8 16 241 270 497 2.68e-12 67.4
MS.gene037545.t1 MTR_4g017440 63.754 309 104 3 4 306 5 311 2.38e-124 365
MS.gene037545.t1 MTR_4g017550 64.685 286 91 5 27 306 3 284 6.53e-119 350
MS.gene037545.t1 MTR_4g017470 54.019 311 98 5 1 306 1 271 5.20e-98 297
MS.gene037545.t1 MTR_4g019310 38.170 317 171 10 1 306 1 303 4.96e-57 191
MS.gene037545.t1 MTR_7g066260 38.188 309 163 9 6 301 8 301 4.15e-56 189
MS.gene037545.t1 MTR_7g066340 37.179 312 165 10 3 301 2 295 1.01e-53 182
MS.gene037545.t1 MTR_7g066340 28.814 236 126 7 6 241 256 449 6.89e-15 75.1
MS.gene037545.t1 MTR_7g066350 36.656 311 171 7 7 306 3 298 1.18e-52 179
MS.gene037545.t1 MTR_7g058710 38.235 306 136 9 16 302 15 286 4.54e-52 178
MS.gene037545.t1 MTR_7g066570 38.907 311 151 12 4 301 5 289 3.90e-51 177
MS.gene037545.t1 MTR_7g066570 27.984 243 146 6 9 241 253 476 2.84e-13 70.5
MS.gene037545.t1 MTR_1g041315 37.143 315 162 12 9 301 10 310 6.68e-49 170
MS.gene037545.t1 MTR_7g066290 37.987 308 163 9 6 301 8 299 2.53e-47 164
MS.gene037545.t1 MTR_7g012680 35.238 315 143 11 4 301 5 275 2.11e-46 162
MS.gene037545.t1 MTR_4g019240 60.976 123 46 1 1 123 1 121 3.87e-45 152
MS.gene037545.t1 MTR_7g066360 35.714 308 171 10 6 301 7 299 3.85e-42 151
MS.gene037545.t1 MTR_7g066430 36.093 302 158 12 16 301 7 289 4.57e-42 152
MS.gene037545.t1 MTR_7g066430 24.797 246 159 4 7 241 251 481 1.77e-13 70.9
MS.gene037545.t1 MTR_7g063660 35.932 295 156 13 16 301 12 282 9.75e-41 148
MS.gene037545.t1 MTR_7g063880 34.304 309 164 12 1 301 1 278 1.34e-39 144
MS.gene037545.t1 MTR_7g066410 33.654 312 165 11 20 301 17 316 3.79e-39 144
MS.gene037545.t1 MTR_7g066410 26.378 254 155 7 2 241 273 508 2.78e-12 67.4
MS.gene037545.t1 MTR_7g063830 33.651 315 166 12 1 301 1 286 1.33e-37 139
MS.gene037545.t1 MTR_7g058700 33.441 311 128 12 19 301 525 784 4.59e-37 140
MS.gene037545.t1 MTR_7g066480 35.714 294 164 11 16 301 19 295 8.76e-37 136
MS.gene037545.t1 MTR_7g063900 36.027 297 154 11 16 301 13 284 1.01e-36 137
MS.gene037545.t1 MTR_7g063890 33.788 293 165 9 16 301 30 300 1.63e-36 137
MS.gene037545.t1 MTR_7g066580 33.562 292 138 10 20 301 2 247 2.04e-36 135
MS.gene037545.t1 MTR_7g066580 28.163 245 148 7 10 241 212 441 4.22e-11 63.5
MS.gene037545.t1 MTR_2g029400 35.319 235 95 3 9 243 10 187 3.77e-36 134
MS.gene037545.t1 MTR_7g066400 33.223 301 147 10 15 302 14 273 1.14e-35 134
MS.gene037545.t1 MTR_7g066400 26.582 237 139 4 5 241 232 433 7.08e-11 63.2
MS.gene037545.t1 MTR_7g063820 37.667 300 147 14 16 301 13 286 2.47e-35 133
MS.gene037545.t1 MTR_7g063840 32.573 307 169 12 6 301 3 282 7.51e-35 132
MS.gene037545.t1 MTR_7g062300 32.903 310 172 10 2 301 5 288 1.08e-34 131
MS.gene037545.t1 MTR_7g062280 33.548 310 158 12 16 301 13 298 6.08e-34 129
MS.gene037545.t1 MTR_7g066490 32.881 295 155 10 16 300 19 280 8.24e-33 125
MS.gene037545.t1 MTR_1g014060 34.802 227 125 6 22 241 9 219 3.12e-31 120
MS.gene037545.t1 MTR_7g063920 32.237 304 150 11 1 302 1 250 3.76e-28 113
MS.gene037545.t1 MTR_3g065200 30.743 296 148 11 16 306 6 249 2.00e-27 111
MS.gene037545.t1 MTR_7g066310 30.333 300 121 9 16 306 10 230 1.77e-26 108
MS.gene037545.t1 MTR_7g063670 31.348 319 163 12 1 301 4 284 1.82e-25 105
MS.gene037545.t1 MTR_3g007740 31.186 295 164 10 16 301 12 276 3.51e-25 104
MS.gene037545.t1 MTR_7g062270 36.735 196 94 9 8 193 5 180 2.55e-21 93.6
MS.gene037545.t1 MTR_7g066280 30.332 211 109 9 100 301 1 182 2.77e-16 79.0
MS.gene037545.t1 MTR_8g445860 28.049 246 144 6 11 243 11 236 3.32e-16 77.0
MS.gene037545.t1 MTR_1g115440 27.848 237 149 6 16 243 14 237 3.87e-15 73.9
MS.gene037545.t1 MTR_6g006800 72.917 48 11 1 1 48 1 46 1.75e-14 67.8
MS.gene037545.t1 MTR_1g115455 27.823 248 131 8 16 243 12 231 7.57e-14 70.1
MS.gene037545.t1 MTR_0150s0010 27.984 243 141 7 16 243 13 236 3.00e-12 65.5
MS.gene037545.t1 MTR_1g115435 26.160 237 144 6 11 247 5 210 6.79e-12 64.3
MS.gene037545.t1 MTR_7g063720 25.641 234 133 8 14 241 24 222 8.63e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037545.t1 AT3G09110 33.663 303 177 9 10 304 6 292 1.04e-42 150
MS.gene037545.t1 AT3G09140 31.677 322 189 9 7 306 3 315 8.92e-39 143
MS.gene037545.t1 AT5G43240 33.115 305 179 10 16 306 12 305 3.41e-37 139
MS.gene037545.t1 AT5G43240 33.115 305 179 10 16 306 12 305 3.41e-37 139
MS.gene037545.t1 AT5G43240 33.115 305 179 10 16 306 12 305 3.41e-37 139
MS.gene037545.t1 AT5G01130 31.629 313 168 12 6 302 3 285 1.95e-34 130
MS.gene037545.t1 AT3G09120 33.333 315 171 13 10 306 3 296 4.57e-34 127
MS.gene037545.t1 AT5G01150 30.868 311 191 9 7 306 3 300 9.58e-34 129
MS.gene037545.t1 AT3G09140 29.143 350 189 10 7 306 3 343 4.54e-33 127
MS.gene037545.t1 AT5G01120 29.870 308 196 7 10 306 6 304 7.77e-33 127
MS.gene037545.t1 AT5G01140 30.744 309 171 9 7 306 3 277 5.03e-29 115
MS.gene037545.t1 AT5G37320 31.879 298 177 12 20 302 18 304 6.34e-26 107

Find 59 sgRNAs with CRISPR-Local

Find 103 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTTACGAATACAATTT+TGG 0.148306 4.3:-82025353 MS.gene037545:CDS
TAATAGACAACCTAAGAATT+TGG 0.262420 4.3:-82026113 MS.gene037545:CDS
CAATTATAAGATAAATAAAA+TGG 0.268027 4.3:-82025269 MS.gene037545:CDS
CGTTGTCTGAGTTGAGGTTT+TGG 0.285726 4.3:+82026317 None:intergenic
CAGGGGAATCATGCCTCTAA+TGG 0.286513 4.3:-82026079 MS.gene037545:CDS
CCCTCAGAGTCCAAATTCTT+AGG 0.309465 4.3:+82026103 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.310647 4.3:+82026115 None:intergenic
ATTCTTCTCCATCAACATAT+TGG 0.310876 4.3:+82025296 None:intergenic
TCAGAATCATCACCATTCTT+TGG 0.319703 4.3:-82026367 MS.gene037545:CDS
ATGCAGATGTTGCTGGAATC+AGG 0.320991 4.3:-82025977 MS.gene037545:CDS
TCCAAAATTGTATTCGTAAG+AGG 0.327361 4.3:+82025352 None:intergenic
AGCAACATTTGCTTACATAA+AGG 0.329982 4.3:+82026283 None:intergenic
TGTTGTTGGTGGTTGCTAAA+AGG 0.334739 4.3:+82026407 None:intergenic
GCAACATTTGCTTACATAAA+GGG 0.341137 4.3:+82026284 None:intergenic
TGTATCAAACACCCCATTAG+AGG 0.344943 4.3:+82026066 None:intergenic
ATATGTTGATGGAGAAGAAT+TGG 0.357603 4.3:-82025293 MS.gene037545:CDS
CAGACATAAAACTTACTTGA+TGG 0.362318 4.3:+82026196 None:intergenic
AGGGGAATCATGCCTCTAAT+GGG 0.380156 4.3:-82026078 MS.gene037545:CDS
ACCTCAACTCAGACAACGTT+TGG 0.380712 4.3:-82026312 MS.gene037545:CDS
GAACTCAATGCAGATGTTGC+TGG 0.386915 4.3:-82025984 MS.gene037545:CDS
GAATTTGGACTCTGAGGGTC+AGG 0.397162 4.3:-82026098 MS.gene037545:CDS
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.3:-82026442 MS.gene037545:CDS
AAGAGGCTCATGAGAGGTTA+AGG 0.445820 4.3:+82025369 None:intergenic
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.3:+82026437 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4.3:+82025380 None:intergenic
TATGTTGATGGAGAAGAATT+GGG 0.477595 4.3:-82025292 MS.gene037545:CDS
CCACGGAACCAATATGTTGA+TGG 0.488534 4.3:-82025304 MS.gene037545:CDS
TTGTCGATCATTGTTGTTGG+TGG 0.499724 4.3:+82026396 None:intergenic
ATTGAGTTCAGCAAGGAACT+TGG 0.501314 4.3:+82025998 None:intergenic
TAGTCAGGAATGGATCCTAA+TGG 0.510661 4.3:+82025156 None:intergenic
CACACAAAGCATTGGCAAGA+AGG 0.511399 4.3:-82025924 MS.gene037545:CDS
CGAAACCTTGACTGCACCTG+CGG 0.512942 4.3:-82026145 MS.gene037545:CDS
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4.3:+82026118 None:intergenic
AGGAATGGATCCTAATGGTA+AGG 0.535365 4.3:+82025161 None:intergenic
TCCAAACGTTGTCTGAGTTG+AGG 0.536321 4.3:+82026311 None:intergenic
CCATCAACATATTGGTTCCG+TGG 0.536459 4.3:+82025304 None:intergenic
TGCAGATGTTGCTGGAATCA+GGG 0.536790 4.3:-82025976 MS.gene037545:CDS
TGTTTGATGTTGCCAAAGAA+TGG 0.539653 4.3:+82026355 None:intergenic
TTGTTCTTGTTGAGAAGTCA+TGG 0.547094 4.3:+82026567 None:intergenic
ATCATCATTGCAGAAGCTGA+TGG 0.549559 4.3:-82025211 MS.gene037545:CDS
ATTCATGTGAATCACTATGC+AGG 0.552422 4.3:-82026249 MS.gene037545:CDS
CTAAGTCCACTAGTCAGGAA+TGG 0.565309 4.3:+82025146 None:intergenic
ACCTAAGAATTTGGACTCTG+AGG 0.575501 4.3:-82026104 MS.gene037545:CDS
AGTGATTCACATGAATTCCT+CGG 0.577391 4.3:+82026256 None:intergenic
AGCAAATGTTGCTGCATCCG+AGG 0.591888 4.3:-82026273 MS.gene037545:CDS
CCTAAGAATTTGGACTCTGA+GGG 0.592717 4.3:-82026103 MS.gene037545:CDS
AATTTGGACTCTGAGGGTCA+GGG 0.595588 4.3:-82026097 MS.gene037545:CDS
ATTTGGACTCTGAGGGTCAG+GGG 0.598326 4.3:-82026096 MS.gene037545:CDS
GAGGAAGTCACACAAAGCAT+TGG 0.599245 4.3:-82025932 MS.gene037545:CDS
AACGTTTCCCCTCAAAATCA+CGG 0.610398 4.3:-82026530 MS.gene037545:CDS
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4.3:+82025383 None:intergenic
TTCAGACTCAACGCAACTAG+AGG 0.625063 4.3:-82025951 MS.gene037545:CDS
GGGGAATCATGCCTCTAATG+GGG 0.639572 4.3:-82026077 MS.gene037545:CDS
CAAAGAACAATGACGAACCA+CGG 0.642115 4.3:-82025321 MS.gene037545:CDS
ACAAAGCATTGGCAAGAAGG+AGG 0.647102 4.3:-82025921 MS.gene037545:intron
GGTTGCTAAAAGGCGAACGA+TGG 0.680679 4.3:+82026417 None:intergenic
ATTCGTAAGAGGCTCATGAG+AGG 0.692406 4.3:+82025363 None:intergenic
CATCTGCATTGAGTTCAGCA+AGG 0.698278 4.3:+82025991 None:intergenic
AGGCGAACGATGGTACCAAG+TGG 0.729828 4.3:+82026427 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATAAGATAAATAAAA+TGG - chr4.3:82026455-82026474 MS.gene037545:CDS 10.0%
!! ATAAAATGTTAAAACACAAA+TGG + chr4.3:82026309-82026328 None:intergenic 15.0%
!!! AAATTTAAAATCATAGGTTA+AGG + chr4.3:82025947-82025966 None:intergenic 15.0%
!!! TAACCTATGATTTTAAATTT+AGG - chr4.3:82025947-82025966 MS.gene037545:CDS 15.0%
!! AATAAAGAGAAATAGAACTT+TGG + chr4.3:82026240-82026259 None:intergenic 20.0%
!! GGATATATATAGTTAATACT+TGG - chr4.3:82026089-82026108 MS.gene037545:CDS 20.0%
!!! GATTTTATTGACTTCATTTT+TGG - chr4.3:82026537-82026556 MS.gene037545:CDS 20.0%
! GGAAAAAAAATCAATGGAAT+GGG + chr4.3:82025887-82025906 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG - chr4.3:82025611-82025630 MS.gene037545:intron 25.0%
! TGGAAAAAAAATCAATGGAA+TGG + chr4.3:82025888-82025907 None:intergenic 25.0%
! TTTCGTTGTGAATCTATATT+GGG - chr4.3:82025975-82025994 MS.gene037545:CDS 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG + chr4.3:82025612-82025631 None:intergenic 25.0%
!! GCACCTAAATTTAAAATCAT+AGG + chr4.3:82025953-82025972 None:intergenic 25.0%
!! TATCTAAAACCATTACATTG+TGG - chr4.3:82026045-82026064 MS.gene037545:CDS 25.0%
AAGAGAAATAGAACTTTGGT+AGG + chr4.3:82026236-82026255 None:intergenic 30.0%
AGAGAAATAGAACTTTGGTA+GGG + chr4.3:82026235-82026254 None:intergenic 30.0%
AGCAACATTTGCTTACATAA+AGG + chr4.3:82025444-82025463 None:intergenic 30.0%
ATCTATATTGGGGTAAAATG+CGG - chr4.3:82025986-82026005 MS.gene037545:CDS 30.0%
ATGTTATCTGTTAGTGATAG+AGG - chr4.3:82026127-82026146 MS.gene037545:CDS 30.0%
ATTCTTCTCCATCAACATAT+TGG + chr4.3:82026431-82026450 None:intergenic 30.0%
CAAATGGTTAGAATGAAAGT+TGG + chr4.3:82026293-82026312 None:intergenic 30.0%
CAGACATAAAACTTACTTGA+TGG + chr4.3:82025531-82025550 None:intergenic 30.0%
CTGCAATGACAATATTTGTT+AGG - chr4.3:82026068-82026087 MS.gene037545:CDS 30.0%
GCAACATTTGCTTACATAAA+GGG + chr4.3:82025443-82025462 None:intergenic 30.0%
GCAAGTGGAAAAAAAATCAA+TGG + chr4.3:82025893-82025912 None:intergenic 30.0%
GTTTCGTTGTGAATCTATAT+TGG - chr4.3:82025974-82025993 MS.gene037545:CDS 30.0%
TCCAAAATTGTATTCGTAAG+AGG + chr4.3:82026375-82026394 None:intergenic 30.0%
TTCGTTGTGAATCTATATTG+GGG - chr4.3:82025976-82025995 MS.gene037545:CDS 30.0%
! ATATGTTGATGGAGAAGAAT+TGG - chr4.3:82026431-82026450 MS.gene037545:CDS 30.0%
! TATGTTGATGGAGAAGAATT+GGG - chr4.3:82026432-82026451 MS.gene037545:CDS 30.0%
! TTTTTGTCGATCATTGTTGT+TGG + chr4.3:82025334-82025353 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG - chr4.3:82025227-82025246 MS.gene037545:CDS 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG - chr4.3:82025851-82025870 MS.gene037545:intron 30.0%
!!! TTTTTGGTTTCCTTACCATT+AGG - chr4.3:82026553-82026572 MS.gene037545:CDS 30.0%
AGTGATTCACATGAATTCCT+CGG + chr4.3:82025471-82025490 None:intergenic 35.0%
ATTCATGTGAATCACTATGC+AGG - chr4.3:82025475-82025494 MS.gene037545:intron 35.0%
TCAGAATCATCACCATTCTT+TGG - chr4.3:82025357-82025376 MS.gene037545:CDS 35.0%
TGTTTGATGTTGCCAAAGAA+TGG + chr4.3:82025372-82025391 None:intergenic 35.0%
! GCCTCTTACGAATACAATTT+TGG - chr4.3:82026371-82026390 MS.gene037545:CDS 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG + chr4.3:82025609-82025628 None:intergenic 35.0%
! TTTGTCTACCGTGATTTTGA+GGG + chr4.3:82025205-82025224 None:intergenic 35.0%
!! ATAGAGGTGTCATTAGGAAA+GGG - chr4.3:82026143-82026162 MS.gene037545:CDS 35.0%
!! CTTGTTTCTACAATGACCTA+AGG + chr4.3:82025922-82025941 None:intergenic 35.0%
!! TTAGTGATAGAGGTGTCATT+AGG - chr4.3:82026137-82026156 MS.gene037545:CDS 35.0%
!!! AGTTGAGGTTTTGGACAATT+TGG + chr4.3:82025401-82025420 None:intergenic 35.0%
!!! GGTTTTGGACAATTTGGTAA+AGG + chr4.3:82025395-82025414 None:intergenic 35.0%
AACGTTTCCCCTCAAAATCA+CGG - chr4.3:82025194-82025213 MS.gene037545:CDS 40.0%
ACCTAAGAATTTGGACTCTG+AGG - chr4.3:82025620-82025639 MS.gene037545:intron 40.0%
AGGAATGGATCCTAATGGTA+AGG + chr4.3:82026566-82026585 None:intergenic 40.0%
AGGTTAAGGTATACTTCAGG+AGG + chr4.3:82026344-82026363 None:intergenic 40.0%
AGTTAGAACTTAGAGGAGAC+TGG - chr4.3:82026170-82026189 MS.gene037545:CDS 40.0%
ATCATCATTGCAGAAGCTGA+TGG - chr4.3:82026513-82026532 MS.gene037545:CDS 40.0%
ATTGAGTTCAGCAAGGAACT+TGG + chr4.3:82025729-82025748 None:intergenic 40.0%
CAAAGAACAATGACGAACCA+CGG - chr4.3:82026403-82026422 MS.gene037545:CDS 40.0%
CCTAAGAATTTGGACTCTGA+GGG - chr4.3:82025621-82025640 MS.gene037545:intron 40.0%
GAAATAGAACTTTGGTAGGG+TGG + chr4.3:82026232-82026251 None:intergenic 40.0%
GAGAGGTTAAGGTATACTTC+AGG + chr4.3:82026347-82026366 None:intergenic 40.0%
GGGAACTAGTTAGAACTTAG+AGG - chr4.3:82026163-82026182 MS.gene037545:CDS 40.0%
TACCTCGACAACGACATTAA+TGG + chr4.3:82026027-82026046 None:intergenic 40.0%
TCAATGGAATGGGTGCATAA+TGG + chr4.3:82025877-82025896 None:intergenic 40.0%
TCATTGCAGCCACAATGTAA+TGG + chr4.3:82026057-82026076 None:intergenic 40.0%
TGTATCAAACACCCCATTAG+AGG + chr4.3:82025661-82025680 None:intergenic 40.0%
TTCCACTTGCAAATGACCTT+AGG - chr4.3:82025903-82025922 MS.gene037545:intron 40.0%
! GTTTGTCTACCGTGATTTTG+AGG + chr4.3:82025206-82025225 None:intergenic 40.0%
! TTGTCGATCATTGTTGTTGG+TGG + chr4.3:82025331-82025350 None:intergenic 40.0%
! TTGTCTACCGTGATTTTGAG+GGG + chr4.3:82025204-82025223 None:intergenic 40.0%
!! GATAGAGGTGTCATTAGGAA+AGG - chr4.3:82026142-82026161 MS.gene037545:CDS 40.0%
!! TGTTGTTGGTGGTTGCTAAA+AGG + chr4.3:82025320-82025339 None:intergenic 40.0%
AAGAGGCTCATGAGAGGTTA+AGG + chr4.3:82026358-82026377 None:intergenic 45.0%
ACCTCAACTCAGACAACGTT+TGG - chr4.3:82025412-82025431 MS.gene037545:intron 45.0%
ACTTTGGTAGGGTGGAACAA+TGG + chr4.3:82026224-82026243 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG - chr4.3:82025282-82025301 MS.gene037545:CDS 45.0%
AGGGGAATCATGCCTCTAAT+GGG - chr4.3:82025646-82025665 MS.gene037545:intron 45.0%
ATGCAGATGTTGCTGGAATC+AGG - chr4.3:82025747-82025766 MS.gene037545:intron 45.0%
ATTCGTAAGAGGCTCATGAG+AGG + chr4.3:82026364-82026383 None:intergenic 45.0%
CATCTGCATTGAGTTCAGCA+AGG + chr4.3:82025736-82025755 None:intergenic 45.0%
CCACGGAACCAATATGTTGA+TGG - chr4.3:82026420-82026439 MS.gene037545:CDS 45.0%
CCCTCAGAGTCCAAATTCTT+AGG + chr4.3:82025624-82025643 None:intergenic 45.0%
GAACTCAATGCAGATGTTGC+TGG - chr4.3:82025740-82025759 MS.gene037545:intron 45.0%
GACCTAAGGTCATTTGCAAG+TGG + chr4.3:82025908-82025927 None:intergenic 45.0%
GAGGAAGTCACACAAAGCAT+TGG - chr4.3:82025792-82025811 MS.gene037545:intron 45.0%
GGGTGCATAATGGCCTAAAA+TGG + chr4.3:82025867-82025886 None:intergenic 45.0%
TCCAAACGTTGTCTGAGTTG+AGG + chr4.3:82025416-82025435 None:intergenic 45.0%
TGCAGATGTTGCTGGAATCA+GGG - chr4.3:82025748-82025767 MS.gene037545:intron 45.0%
TGCCATTAATGTCGTTGTCG+AGG - chr4.3:82026022-82026041 MS.gene037545:CDS 45.0%
TGGCAAGAAGGAGGTAACTA+AGG - chr4.3:82025812-82025831 MS.gene037545:intron 45.0%
TGGTACCAAGTGGCAAAGAT+AGG + chr4.3:82025290-82025309 None:intergenic 45.0%
TTCAGACTCAACGCAACTAG+AGG - chr4.3:82025773-82025792 MS.gene037545:intron 45.0%
! AATTTGGACTCTGAGGGTCA+GGG - chr4.3:82025627-82025646 MS.gene037545:intron 45.0%
! TCTATTAGGAGGTTTTCCGC+AGG + chr4.3:82025598-82025617 None:intergenic 45.0%
!! ACAAAGCATTGGCAAGAAGG+AGG - chr4.3:82025803-82025822 MS.gene037545:intron 45.0%
!! CACACAAAGCATTGGCAAGA+AGG - chr4.3:82025800-82025819 MS.gene037545:intron 45.0%
!! CCATCAACATATTGGTTCCG+TGG + chr4.3:82026423-82026442 None:intergenic 45.0%
!! CGTTGTCTGAGTTGAGGTTT+TGG + chr4.3:82025410-82025429 None:intergenic 45.0%
AGCAAATGTTGCTGCATCCG+AGG - chr4.3:82025451-82025470 MS.gene037545:intron 50.0%
CAGGGGAATCATGCCTCTAA+TGG - chr4.3:82025645-82025664 MS.gene037545:intron 50.0%
GGGGAATCATGCCTCTAATG+GGG - chr4.3:82025647-82025666 MS.gene037545:intron 50.0%
GGTTGCTAAAAGGCGAACGA+TGG + chr4.3:82025310-82025329 None:intergenic 50.0%
! ATTTGGACTCTGAGGGTCAG+GGG - chr4.3:82025628-82025647 MS.gene037545:intron 50.0%
! GAATTTGGACTCTGAGGGTC+AGG - chr4.3:82025626-82025645 MS.gene037545:intron 50.0%
AGGCGAACGATGGTACCAAG+TGG + chr4.3:82025300-82025319 None:intergenic 55.0%
CGAAACCTTGACTGCACCTG+CGG - chr4.3:82025579-82025598 MS.gene037545:intron 55.0%
! GTTTTCCGCAGGTGCAGTCA+AGG + chr4.3:82025587-82025606 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82025159 82026587 82025159 ID=MS.gene037545
chr4.3 mRNA 82025159 82026587 82025159 ID=MS.gene037545.t1;Parent=MS.gene037545
chr4.3 exon 82025922 82026587 82025922 ID=MS.gene037545.t1.exon1;Parent=MS.gene037545.t1
chr4.3 CDS 82025922 82026587 82025922 ID=cds.MS.gene037545.t1;Parent=MS.gene037545.t1
chr4.3 exon 82025159 82025413 82025159 ID=MS.gene037545.t1.exon2;Parent=MS.gene037545.t1
chr4.3 CDS 82025159 82025413 82025159 ID=cds.MS.gene037545.t1;Parent=MS.gene037545.t1
Gene Sequence

>MS.gene037545

ATGACTTCTCAACAAGAACAACAAGAAGAGTCAAAAACGTTTCCCCTCAAAATCACGGTAGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGAGACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCAACCACCAACAACAATGATCGACAAAAACTCTCAGAATCATCACCATTCTTTGGCAACATCAAACACCTTTACCAAATTGTCCAAAACCTCAACTCAGACAACGTTTGGAACAACCCTTTATGTAAGCAAATGTTGCTGCATCCGAGGAATTCATGTGAATCACTATGCAGGAAGTTGTACTTGAATATTGATGATTCTGAACCATCAAGTAAGTTTTATGTCTGTGATTCTTGTAACAAGTTCACTATTTTTCGAAACCTTGACTGCACCTGCGGAAAACCTCCTAATAGACAACCTAAGAATTTGGACTCTGAGGGTCAGGGGAATCATGCCTCTAATGGGGTGTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAATATTGTGCCAAGTTCCTTGCTGAACTCAATGCAGATGTTGCTGGAATCAGGGATTTCAGACTCAACGCAACTAGAGGAAGTCACACAAAGCATTGGCAAGAAGGAGGTAACTAAGGATGTTGTGTTGTTTTTGTTTGTTGTGTTTCCATTTTAGGCCATTATGCACCCATTCCATTGATTTTTTTTCCACTTGCAAATGACCTTAGGTCATTGTAGAAACAAGATCCTTAACCTATGATTTTAAATTTAGGTGCAGTTTCGTTGTGAATCTATATTGGGGTAAAATGCGGTCGTATTTGCAGTTGCCATTAATGTCGTTGTCGAGGTATCTAAAACCATTACATTGTGGCTGCAATGACAATATTTGTTAGGATATATATAGTTAATACTTGGATTTGACATAGTATGATGTTATCTGTTAGTGATAGAGGTGTCATTAGGAAAGGGAACTAGTTAGAACTTAGAGGAGACTGGTAAATATACAATTTACTCTTGTTGTGTTCCATTGTTCCACCCTACCAAAGTTCTATTTCTCTTTATTCAAGACTCTCTCAAGTATAATTTCTAAACTCCAACTTTCATTCTAACCATTTGTGTTTTAACATTTTATACAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACGAATACAATTTTGGAAAGTAGCTCAAAGAACAATGACGAACCACGGAACCAATATGTTGATGGAGAAGAATTGGGCAATTATAAGATAAATAAAATGGATATTAAAGTATTGCAAAGCAAATCTCAGAATAAGATCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTGGTTTCCTTACCATTAGGATCCATTCCTGA
Protein sequence

>MS.gene037545.t1

MTSQQEQQEESKTFPLKITVDKQSNKVVFVEATKDFVETLFSFLSLPLGTIVRLLATTNNNDRQKLSESSPFFGNIKHLYQIVQNLNSDNVWNNPLCKQMLLHPRNSCESLCRKLYLNIDDSEPSSKFYVCDSCNKFTIFRNLDCTCGKPPNRQPKNLDSEGQGNHASNGVFDTESEQMFLVSDDLNIVPSSLLNSMQMLLESGISDSTQLEEVTQSIGKKEILNLLKYTLTSHEPLTNTILESSSKNNDEPRNQYVDGEELGNYKINKMDIKVLQSKSQNKIIIAEADGDFIDFIFGFLTIRIHS