AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89823

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89823.t1 MTR_7g063890 94.841 504 25 1 1 503 1 504 0.0 919
MS.gene89823.t1 MTR_7g063900 73.211 489 127 4 19 504 2 489 0.0 687
MS.gene89823.t1 MTR_7g063840 56.939 490 191 11 19 504 1 474 4.41e-169 486
MS.gene89823.t1 MTR_7g062280 53.908 499 197 11 25 504 8 492 3.93e-163 471
MS.gene89823.t1 MTR_7g063660 55.785 484 198 10 25 504 7 478 9.69e-162 467
MS.gene89823.t1 MTR_7g062300 55.094 481 190 12 25 497 10 472 1.37e-160 464
MS.gene89823.t1 MTR_7g063880 54.792 480 185 11 24 497 8 461 4.83e-158 457
MS.gene89823.t1 MTR_7g063820 57.028 498 176 15 25 504 8 485 7.54e-156 452
MS.gene89823.t1 MTR_7g063830 52.469 486 174 11 19 497 2 437 8.09e-150 436
MS.gene89823.t1 MTR_7g063920 52.772 487 169 11 23 504 7 437 6.15e-148 431
MS.gene89823.t1 MTR_3g007740 49.491 491 186 12 19 497 1 441 2.45e-128 381
MS.gene89823.t1 MTR_7g063670 46.964 494 200 15 25 504 13 458 1.03e-115 349
MS.gene89823.t1 MTR_7g066570 42.886 492 236 12 26 497 13 479 3.96e-110 338
MS.gene89823.t1 MTR_7g058710 39.961 508 254 12 20 504 5 484 6.95e-103 317
MS.gene89823.t1 MTR_7g066260 42.623 488 222 14 26 496 14 460 5.15e-102 315
MS.gene89823.t1 MTR_3g065200 42.641 462 206 12 27 485 3 408 2.62e-94 294
MS.gene89823.t1 MTR_7g012680 40.388 515 233 16 1 504 1 452 1.78e-93 291
MS.gene89823.t1 MTR_7g066430 39.676 494 274 11 27 503 4 490 1.49e-91 288
MS.gene89823.t1 MTR_7g066430 26.609 233 152 4 23 242 253 479 1.14e-15 79.7
MS.gene89823.t1 MTR_7g066350 38.259 494 244 10 13 494 1 445 3.37e-91 286
MS.gene89823.t1 MTR_7g066350 27.419 248 147 8 268 500 9 238 1.64e-12 69.7
MS.gene89823.t1 MTR_7g066340 38.900 491 232 14 26 497 11 452 4.02e-91 286
MS.gene89823.t1 MTR_7g066410 37.305 512 274 10 25 497 8 511 3.11e-88 280
MS.gene89823.t1 MTR_7g066410 39.130 92 51 1 261 347 3 94 2.23e-12 69.7
MS.gene89823.t1 MTR_1g041315 35.950 484 238 10 26 482 13 451 1.23e-82 265
MS.gene89823.t1 MTR_7g066290 38.398 487 215 11 26 496 14 431 2.14e-82 262
MS.gene89823.t1 MTR_4g019310 35.913 504 280 14 21 497 2 489 1.98e-81 262
MS.gene89823.t1 MTR_7g066580 38.046 481 241 14 33 497 1 440 1.43e-80 258
MS.gene89823.t1 MTR_7g066580 27.126 247 141 8 21 243 209 440 3.09e-13 72.0
MS.gene89823.t1 MTR_7g066360 38.923 483 229 11 28 495 15 446 5.43e-79 255
MS.gene89823.t1 MTR_7g066400 36.082 485 230 12 27 494 12 433 1.23e-76 248
MS.gene89823.t1 MTR_7g066400 24.670 227 144 6 18 242 230 431 1.27e-13 73.2
MS.gene89823.t1 MTR_1g014060 36.017 472 202 14 40 504 13 391 7.76e-74 239
MS.gene89823.t1 MTR_7g063720 52.381 252 90 6 248 497 2 225 7.81e-73 231
MS.gene89823.t1 MTR_7g063720 28.033 239 146 8 10 242 2 220 4.66e-18 84.0
MS.gene89823.t1 MTR_7g066480 42.651 347 177 9 26 360 15 351 1.27e-70 231
MS.gene89823.t1 MTR_7g066310 34.884 473 209 12 26 496 6 381 1.06e-68 226
MS.gene89823.t1 MTR_2g029400 33.902 469 226 9 26 487 13 404 4.56e-68 224
MS.gene89823.t1 MTR_7g066490 41.337 329 163 6 21 339 12 320 1.39e-65 218
MS.gene89823.t1 MTR_4g017830 32.549 510 282 16 28 497 13 500 2.79e-65 220
MS.gene89823.t1 MTR_4g017440 32.302 517 299 15 21 500 2 504 2.04e-62 212
MS.gene89823.t1 MTR_7g058700 38.938 339 156 6 39 355 531 840 2.65e-61 216
MS.gene89823.t1 MTR_4g017410 30.680 515 295 13 19 500 1 486 4.86e-59 203
MS.gene89823.t1 MTR_4g017610 31.187 497 273 15 30 494 16 475 5.00e-58 199
MS.gene89823.t1 MTR_7g063850 43.182 308 150 9 198 503 4 288 7.46e-58 194
MS.gene89823.t1 MTR_4g019340 32.213 506 281 16 16 488 27 503 2.97e-56 196
MS.gene89823.t1 MTR_4g017470 30.604 513 271 13 28 504 13 476 4.20e-55 192
MS.gene89823.t1 MTR_4g017470 25.911 247 150 7 20 241 225 463 8.91e-12 67.8
MS.gene89823.t1 MTR_7g062270 52.778 180 79 4 19 194 1 178 1.17e-52 183
MS.gene89823.t1 MTR_7g062270 36.145 83 48 1 269 346 11 93 8.45e-11 64.3
MS.gene89823.t1 MTR_7g063690 42.403 283 94 8 19 295 3 222 2.41e-52 177
MS.gene89823.t1 MTR_7g100420 40.367 327 171 9 171 494 88 393 3.46e-52 182
MS.gene89823.t1 MTR_7g100420 74.510 51 13 0 20 70 32 82 1.41e-14 75.9
MS.gene89823.t1 MTR_7g066280 31.850 427 216 11 106 496 1 388 1.26e-47 170
MS.gene89823.t1 MTR_8g023240 26.844 488 313 15 18 492 27 483 7.91e-42 157
MS.gene89823.t1 MTR_4g017550 29.878 492 283 18 41 495 4 470 2.54e-41 154
MS.gene89823.t1 MTR_4g017550 28.571 245 136 7 19 241 239 466 1.55e-14 76.3
MS.gene89823.t1 MTR_7g066680 36.774 310 144 8 225 494 37 334 7.03e-40 147
MS.gene89823.t1 MTR_7g066680 26.455 189 120 5 27 205 86 265 7.90e-13 70.1
MS.gene89823.t1 MTR_8g445860 31.330 233 142 6 27 248 13 238 7.91e-27 108
MS.gene89823.t1 MTR_8g445860 29.583 240 132 7 268 491 13 231 6.27e-18 83.6
MS.gene89823.t1 MTR_0150s0010 29.388 245 146 6 20 247 3 237 2.42e-22 96.3
MS.gene89823.t1 MTR_0150s0010 29.614 233 144 7 268 491 10 231 4.99e-18 84.0
MS.gene89823.t1 MTR_1g115440 30.328 244 142 6 20 247 7 238 3.57e-22 95.9
MS.gene89823.t1 MTR_2g067310 36.022 186 77 6 27 208 43 190 1.79e-19 88.2
MS.gene89823.t1 MTR_7g063760 54.795 73 31 2 434 504 2 74 9.54e-19 80.9
MS.gene89823.t1 MTR_1g115455 28.163 245 149 7 19 248 1 233 5.96e-17 80.5
MS.gene89823.t1 MTR_7g063870 64.815 54 19 0 24 77 28 81 7.11e-15 75.1
MS.gene89823.t1 MTR_7g063870 54.688 64 28 1 170 232 94 157 3.46e-12 67.0
MS.gene89823.t1 MTR_1g115450 36.264 91 53 1 24 114 4 89 2.02e-11 62.4
MS.gene89823.t1 MTR_1g115450 36.250 80 51 0 268 347 7 86 9.02e-11 60.5
MS.gene89823.t1 MTR_1g115470 41.414 99 50 3 30 126 9 101 5.15e-11 65.1
MS.gene89823.t1 MTR_7g066390 44.578 83 42 2 414 496 42 120 5.50e-11 61.2
MS.gene89823.t1 MTR_4g019240 33.333 123 69 4 15 129 4 121 7.36e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89823.t1 AT3G09140 33.595 509 281 11 30 503 12 498 7.63e-67 224
MS.gene89823.t1 AT3G09140 31.387 548 291 12 19 503 1 526 5.33e-62 212
MS.gene89823.t1 AT5G01120 34.086 487 286 13 30 494 12 485 6.74e-62 211
MS.gene89823.t1 AT5G01120 25.296 253 163 7 1 241 244 482 8.00e-13 71.2
MS.gene89823.t1 AT5G01150 32.072 502 294 11 19 497 1 478 5.20e-58 200
MS.gene89823.t1 AT5G43240 33.130 492 288 11 30 494 12 489 5.66e-55 192
MS.gene89823.t1 AT5G43240 33.130 492 288 11 30 494 12 489 5.66e-55 192
MS.gene89823.t1 AT5G43240 33.130 492 288 11 30 494 12 489 5.66e-55 192
MS.gene89823.t1 AT5G01130 30.162 494 279 10 19 497 1 443 1.40e-51 182
MS.gene89823.t1 AT5G01140 29.150 494 291 13 19 497 1 450 6.66e-51 181
MS.gene89823.t1 AT3G09110 35.897 312 183 4 30 329 12 318 1.45e-50 176
MS.gene89823.t1 AT3G09120 34.603 315 176 6 26 319 5 310 3.49e-43 155
MS.gene89823.t1 AT3G09120 28.270 237 155 6 263 492 1 229 5.14e-13 70.5
MS.gene89823.t1 AT5G37320 28.350 515 288 18 28 503 12 484 1.11e-37 144
MS.gene89823.t1 AT5G43240 29.464 336 211 8 173 494 22 345 1.55e-18 87.8

Find 73 sgRNAs with CRISPR-Local

Find 92 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TCAACTTTCTTGACACATTT+TGG 0.067658 7.3:+41469567 MS.gene89823:CDS
CGTCACTTAACCAATGTTAA+AGG 0.188086 7.3:+41470771 MS.gene89823:CDS
ATCACTTGCCTTAGGTGATT+TGG 0.194221 7.3:-41470423 None:intergenic
GGCATTATTATTACTATAAT+AGG 0.236736 7.3:+41470279 MS.gene89823:CDS
GGCGGAGTTGTTCGCGTATT+AGG 0.267322 7.3:+41470089 MS.gene89823:CDS
CTAAGCATGCTTCAGAATTC+TGG 0.274857 7.3:+41469669 MS.gene89823:CDS
AACACTTGATCTTCTGTATT+AGG 0.291800 7.3:-41469135 None:intergenic
TAATCTTCAGAAGAGTTCTT+TGG 0.301786 7.3:-41469399 None:intergenic
CTATAATAGGTTCCTAGGTT+TGG 0.323823 7.3:+41470292 MS.gene89823:CDS
ATTAGGAGGGAATTGCTCTT+TGG 0.327353 7.3:+41470106 MS.gene89823:CDS
CAAAGTACTTCATATGTTGC+AGG 0.327554 7.3:+41469463 MS.gene89823:CDS
TCACTTGCCTTAGGTGATTT+GGG 0.328981 7.3:-41470422 None:intergenic
TATAATAGGTTCCTAGGTTT+GGG 0.331462 7.3:+41470293 MS.gene89823:CDS
GTCACTTAACCAATGTTAAA+GGG 0.338237 7.3:+41470772 MS.gene89823:CDS
CAACATATGAAGTACTTTGT+AGG 0.355917 7.3:-41469459 None:intergenic
TCAAGTGTTGTTGAAACTTC+TGG 0.356750 7.3:+41469149 MS.gene89823:CDS
TTCAGACAACCAATGGTAAC+TGG 0.356771 7.3:-41469300 None:intergenic
TCACCTAAGGCAAGTGATAA+TGG 0.365777 7.3:+41470428 MS.gene89823:CDS
GCAGTGTCTGAGCATATTAA+AGG 0.368152 7.3:+41470710 MS.gene89823:intron
TTCAGCAATTAAGACTTTGT+TGG 0.376814 7.3:-41469184 None:intergenic
GTATGTGGTTACAGATGATT+TGG 0.389987 7.3:+41470478 MS.gene89823:CDS
TATTACTATAATAGGTTCCT+AGG 0.390455 7.3:+41470287 MS.gene89823:CDS
TTAGGAGGGAATTGCTCTTT+GGG 0.394467 7.3:+41470107 MS.gene89823:CDS
AATCTTCAGAAGAGTTCTTT+GGG 0.396497 7.3:-41469398 None:intergenic
GGGAAAGGATTTGTTAAAGA+AGG 0.419018 7.3:+41469588 MS.gene89823:CDS
CAAATCTGTCAGAGTAGACT+TGG 0.447463 7.3:-41469886 None:intergenic
AAAGTCTTAATTGCTGAAGC+AGG 0.453365 7.3:+41469189 MS.gene89823:CDS
TGACAAAAGAAGCAAAGAAA+AGG 0.458594 7.3:+41470183 MS.gene89823:CDS
TCATTTCTCCCCTTTAACAT+TGG 0.461782 7.3:-41470781 None:intergenic
ATTTGGGATTACTACTAACT+AGG 0.472685 7.3:-41470406 None:intergenic
TCGTCGTTTGCTCTAACAAA+TGG 0.479714 7.3:+41470744 MS.gene89823:CDS
AGCCCTGTGAATTCTGAGTT+CGG 0.486240 7.3:-41469648 None:intergenic
AGTTTCTTAACATTGCCATC+AGG 0.486390 7.3:+41469237 MS.gene89823:CDS
TGTCTGAACAAACTTTATCA+AGG 0.490886 7.3:+41469315 MS.gene89823:CDS
AAGAGAAAACACTTGACCTC+TGG 0.496645 7.3:-41469932 None:intergenic
TCTCAACGTCACTAAGGAAA+AGG 0.499259 7.3:+41469725 MS.gene89823:CDS
TAACCATTATCACTTGCCTT+AGG 0.502444 7.3:-41470431 None:intergenic
CATAGAGAAGGACTCAAAGA+AGG 0.513614 7.3:+41469275 MS.gene89823:CDS
AAGGAAAAGCTTGTCACCAT+TGG 0.519499 7.3:+41470572 MS.gene89823:CDS
TCTTACCTCCTTGATGCCAA+TGG 0.523413 7.3:-41470588 None:intergenic
GCTTGTCACCATTGGCATCA+AGG 0.526563 7.3:+41470580 MS.gene89823:CDS
GGAGTTGTTCGCGTATTAGG+AGG 0.538074 7.3:+41470092 MS.gene89823:CDS
ACACTTGACCTCTGGATCAA+CGG 0.538261 7.3:-41469924 None:intergenic
GGCAAGATATTGTTTGCTCA+AGG 0.542741 7.3:+41470017 MS.gene89823:CDS
TGTTGATCCACACTTGGCAC+CGG 0.545388 7.3:+41470208 MS.gene89823:CDS
ATGCCGAACTCAGAATTCAC+AGG 0.548559 7.3:+41469645 MS.gene89823:CDS
AATGACTCTCAACGTCACTA+AGG 0.552198 7.3:+41469719 MS.gene89823:CDS
TTTCCTTCACATCATGAAGA+GGG 0.554951 7.3:-41470556 None:intergenic
TTGCTTAATTTGTTGAACTC+CGG 0.560839 7.3:-41470227 None:intergenic
TAGTAATCCCAAATCACCTA+AGG 0.565873 7.3:+41470415 MS.gene89823:CDS
AAGTTTGTTCAGACAACCAA+TGG 0.569049 7.3:-41469307 None:intergenic
AAGAAGGGACCAGTTACCAT+TGG 0.570022 7.3:+41469291 MS.gene89823:CDS
GAGTTGTTCGCGTATTAGGA+GGG 0.571871 7.3:+41470093 MS.gene89823:CDS
AGTCCCTCTTCATGATGTGA+AGG 0.573117 7.3:+41470553 MS.gene89823:CDS
AAAAGAAACCGTTGATCCAG+AGG 0.583692 7.3:+41469916 MS.gene89823:CDS
TTGAACTCCGGTGCCAAGTG+TGG 0.586212 7.3:-41470215 None:intergenic
TTTCTCACATTTCCTTTGGG+CGG 0.589849 7.3:+41470071 MS.gene89823:CDS
AAGTCTTAATTGCTGAAGCA+GGG 0.590272 7.3:+41469190 MS.gene89823:CDS
AATGGTTATGTAAAAGCAGA+GGG 0.594931 7.3:+41470446 MS.gene89823:CDS
ACGCGAACAACTCCGCCCAA+AGG 0.598183 7.3:-41470083 None:intergenic
TACCTATTCAAAAGCTACGT+GGG 0.608030 7.3:+41470258 MS.gene89823:CDS
AAGGCTTGTTGATCCACACT+TGG 0.608575 7.3:+41470202 MS.gene89823:CDS
TAATGCTATTACCCAAACCT+AGG 0.609121 7.3:-41470304 None:intergenic
TCACTTAACCAATGTTAAAG+GGG 0.615960 7.3:+41470773 MS.gene89823:CDS
AGAGGGACACAGAAAGTATG+TGG 0.617669 7.3:+41470463 MS.gene89823:CDS
ATAGAGAAGGACTCAAAGAA+GGG 0.619880 7.3:+41469276 MS.gene89823:CDS
AGTCTTGCAATAGTTCCTGA+TGG 0.633251 7.3:-41469252 None:intergenic
TTATGTGAGAACTGATAGAG+AGG 0.644156 7.3:+41470379 MS.gene89823:CDS
TATTGCAAGACTCATAGAGA+AGG 0.648416 7.3:+41469263 MS.gene89823:CDS
TAATGGTTATGTAAAAGCAG+AGG 0.652935 7.3:+41470445 MS.gene89823:CDS
TGCCGAACTCAGAATTCACA+GGG 0.676332 7.3:+41469646 MS.gene89823:CDS
TTACCTATTCAAAAGCTACG+TGG 0.707634 7.3:+41470257 MS.gene89823:CDS
TGTCACCATTGGCATCAAGG+AGG 0.741971 7.3:+41470583 MS.gene89823:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTGATGATAAAAATTAAA+AGG - chr7.3:41469806-41469825 None:intergenic 10.0%
!!! ATTAAAAATAGATTTGAAAT+TGG - chr7.3:41470521-41470540 None:intergenic 10.0%
!! AAAAAACACAAGTTCAATAA+CGG + chr7.3:41469695-41469714 MS.gene89823:CDS 20.0%
!! AAAGGTTACTATTAAAAAAG+AGG + chr7.3:41469098-41469117 MS.gene89823:CDS 20.0%
!! GGCATTATTATTACTATAAT+AGG + chr7.3:41470279-41470298 MS.gene89823:CDS 20.0%
!! TGATGATAAAAATTAAAAGG+CGG - chr7.3:41469803-41469822 None:intergenic 20.0%
!!! GATTGATTAATATTTTGTAC+AGG + chr7.3:41469833-41469852 MS.gene89823:intron 20.0%
!!! TTAGTTTTTCGAAAATCAAA+TGG + chr7.3:41469996-41470015 MS.gene89823:CDS 20.0%
! TATAAAGCAAGCAACAAATT+AGG - chr7.3:41469776-41469795 None:intergenic 25.0%
! TATTAAAAAAGAGGAGCAAA+AGG + chr7.3:41469080-41469099 MS.gene89823:CDS 25.0%
! TATTACTATAATAGGTTCCT+AGG + chr7.3:41470287-41470306 MS.gene89823:CDS 25.0%
AACACTTGATCTTCTGTATT+AGG - chr7.3:41469138-41469157 None:intergenic 30.0%
AATCTTCAGAAGAGTTCTTT+GGG - chr7.3:41469401-41469420 None:intergenic 30.0%
AATGGTTATGTAAAAGCAGA+GGG + chr7.3:41470446-41470465 MS.gene89823:CDS 30.0%
ACAGACAAAAATTGTGTGTA+TGG + chr7.3:41469528-41469547 MS.gene89823:CDS 30.0%
GTCACTTAACCAATGTTAAA+GGG + chr7.3:41470772-41470791 MS.gene89823:CDS 30.0%
TAATCTTCAGAAGAGTTCTT+TGG - chr7.3:41469402-41469421 None:intergenic 30.0%
TAATGGTTATGTAAAAGCAG+AGG + chr7.3:41470445-41470464 MS.gene89823:CDS 30.0%
TATAATAGGTTCCTAGGTTT+GGG + chr7.3:41470293-41470312 MS.gene89823:CDS 30.0%
TATTAAAAAAGAGGAGCAAG+TGG + chr7.3:41469107-41469126 MS.gene89823:CDS 30.0%
TCACTTAACCAATGTTAAAG+GGG + chr7.3:41470773-41470792 MS.gene89823:CDS 30.0%
TGACAAAAGAAGCAAAGAAA+AGG + chr7.3:41470183-41470202 MS.gene89823:CDS 30.0%
TGTCTGAACAAACTTTATCA+AGG + chr7.3:41469315-41469334 MS.gene89823:CDS 30.0%
TTCAGCAATTAAGACTTTGT+TGG - chr7.3:41469187-41469206 None:intergenic 30.0%
! AAGTTTTCTCACATTTCCTT+TGG + chr7.3:41470067-41470086 MS.gene89823:CDS 30.0%
! AGTTTTCTCACATTTCCTTT+GGG + chr7.3:41470068-41470087 MS.gene89823:CDS 30.0%
! ATTTGGGATTACTACTAACT+AGG - chr7.3:41470409-41470428 None:intergenic 30.0%
! CAACATATGAAGTACTTTGT+AGG - chr7.3:41469462-41469481 None:intergenic 30.0%
! CAACTTTCTTGACACATTTT+GGG + chr7.3:41469568-41469587 MS.gene89823:CDS 30.0%
! TCAACTTTCTTGACACATTT+TGG + chr7.3:41469567-41469586 MS.gene89823:CDS 30.0%
!! TTGCTTAATTTGTTGAACTC+CGG - chr7.3:41470230-41470249 None:intergenic 30.0%
AAAGTCTTAATTGCTGAAGC+AGG + chr7.3:41469189-41469208 MS.gene89823:CDS 35.0%
AAGTCTTAATTGCTGAAGCA+GGG + chr7.3:41469190-41469209 MS.gene89823:CDS 35.0%
AGTTTCTTAACATTGCCATC+AGG + chr7.3:41469237-41469256 MS.gene89823:CDS 35.0%
ATAGAGAAGGACTCAAAGAA+GGG + chr7.3:41469276-41469295 MS.gene89823:CDS 35.0%
CGTCACTTAACCAATGTTAA+AGG + chr7.3:41470771-41470790 MS.gene89823:CDS 35.0%
CTATAATAGGTTCCTAGGTT+TGG + chr7.3:41470292-41470311 MS.gene89823:CDS 35.0%
GTATGTGGTTACAGATGATT+TGG + chr7.3:41470478-41470497 MS.gene89823:CDS 35.0%
TAACCATTATCACTTGCCTT+AGG - chr7.3:41470434-41470453 None:intergenic 35.0%
TAATGCTATTACCCAAACCT+AGG - chr7.3:41470307-41470326 None:intergenic 35.0%
TACCTATTCAAAAGCTACGT+GGG + chr7.3:41470258-41470277 MS.gene89823:CDS 35.0%
TAGTAATCCCAAATCACCTA+AGG + chr7.3:41470415-41470434 MS.gene89823:CDS 35.0%
TATTGCAAGACTCATAGAGA+AGG + chr7.3:41469263-41469282 MS.gene89823:CDS 35.0%
TCATTTCTCCCCTTTAACAT+TGG - chr7.3:41470784-41470803 None:intergenic 35.0%
TTACCTATTCAAAAGCTACG+TGG + chr7.3:41470257-41470276 MS.gene89823:CDS 35.0%
TTATGTGAGAACTGATAGAG+AGG + chr7.3:41470379-41470398 MS.gene89823:CDS 35.0%
TTTCCTTCACATCATGAAGA+GGG - chr7.3:41470559-41470578 None:intergenic 35.0%
! AAGTTTGTTCAGACAACCAA+TGG - chr7.3:41469310-41469329 None:intergenic 35.0%
! CAAAGTACTTCATATGTTGC+AGG + chr7.3:41469463-41469482 MS.gene89823:CDS 35.0%
! TCAAGTGTTGTTGAAACTTC+TGG + chr7.3:41469149-41469168 MS.gene89823:CDS 35.0%
! TTTTCCTTCACATCATGAAG+AGG - chr7.3:41470560-41470579 None:intergenic 35.0%
!! GGGAAAGGATTTGTTAAAGA+AGG + chr7.3:41469588-41469607 MS.gene89823:CDS 35.0%
!! TTCTTGACACATTTTGGGAA+AGG + chr7.3:41469573-41469592 MS.gene89823:CDS 35.0%
AAAAGAAACCGTTGATCCAG+AGG + chr7.3:41469916-41469935 MS.gene89823:CDS 40.0%
AAACCAAAAAACACGACGCA+TGG - chr7.3:41470641-41470660 None:intergenic 40.0%
AAGAGAAAACACTTGACCTC+TGG - chr7.3:41469935-41469954 None:intergenic 40.0%
AAGGAAAAGCTTGTCACCAT+TGG + chr7.3:41470572-41470591 MS.gene89823:CDS 40.0%
AATGACTCTCAACGTCACTA+AGG + chr7.3:41469719-41469738 MS.gene89823:CDS 40.0%
AGTCTTGCAATAGTTCCTGA+TGG - chr7.3:41469255-41469274 None:intergenic 40.0%
ATCACTTGCCTTAGGTGATT+TGG - chr7.3:41470426-41470445 None:intergenic 40.0%
ATTAGGAGGGAATTGCTCTT+TGG + chr7.3:41470106-41470125 MS.gene89823:CDS 40.0%
CAAATCTGTCAGAGTAGACT+TGG - chr7.3:41469889-41469908 None:intergenic 40.0%
CATAGAGAAGGACTCAAAGA+AGG + chr7.3:41469275-41469294 MS.gene89823:CDS 40.0%
CTAAGCATGCTTCAGAATTC+TGG + chr7.3:41469669-41469688 MS.gene89823:CDS 40.0%
GCAGTGTCTGAGCATATTAA+AGG + chr7.3:41470710-41470729 MS.gene89823:intron 40.0%
GGCAAGATATTGTTTGCTCA+AGG + chr7.3:41470017-41470036 MS.gene89823:CDS 40.0%
TCACCTAAGGCAAGTGATAA+TGG + chr7.3:41470428-41470447 MS.gene89823:CDS 40.0%
TCACTTGCCTTAGGTGATTT+GGG - chr7.3:41470425-41470444 None:intergenic 40.0%
TCGTCGTTTGCTCTAACAAA+TGG + chr7.3:41470744-41470763 MS.gene89823:CDS 40.0%
TCTCAACGTCACTAAGGAAA+AGG + chr7.3:41469725-41469744 MS.gene89823:CDS 40.0%
TTAGGAGGGAATTGCTCTTT+GGG + chr7.3:41470107-41470126 MS.gene89823:CDS 40.0%
TTCAGACAACCAATGGTAAC+TGG - chr7.3:41469303-41469322 None:intergenic 40.0%
TTTCTCACATTTCCTTTGGG+CGG + chr7.3:41470071-41470090 MS.gene89823:CDS 40.0%
AAGAAGGGACCAGTTACCAT+TGG + chr7.3:41469291-41469310 MS.gene89823:CDS 45.0%
ACACTTGACCTCTGGATCAA+CGG - chr7.3:41469927-41469946 None:intergenic 45.0%
AGCCCTGTGAATTCTGAGTT+CGG - chr7.3:41469651-41469670 None:intergenic 45.0%
AGTCCCTCTTCATGATGTGA+AGG + chr7.3:41470553-41470572 MS.gene89823:CDS 45.0%
ATGCCGAACTCAGAATTCAC+AGG + chr7.3:41469645-41469664 MS.gene89823:CDS 45.0%
GAGTTGTTCGCGTATTAGGA+GGG + chr7.3:41470093-41470112 MS.gene89823:CDS 45.0%
TCTTACCTCCTTGATGCCAA+TGG - chr7.3:41470591-41470610 None:intergenic 45.0%
TGCCGAACTCAGAATTCACA+GGG + chr7.3:41469646-41469665 MS.gene89823:CDS 45.0%
! AGAGGGACACAGAAAGTATG+TGG + chr7.3:41470463-41470482 MS.gene89823:CDS 45.0%
! TGCCCACGTAGCTTTTGAAT+AGG - chr7.3:41470263-41470282 None:intergenic 45.0%
!! AAGGCTTGTTGATCCACACT+TGG + chr7.3:41470202-41470221 MS.gene89823:CDS 45.0%
!!! TCACCATGCGTCGTGTTTTT+TGG + chr7.3:41470635-41470654 MS.gene89823:intron 45.0%
GCTTGTCACCATTGGCATCA+AGG + chr7.3:41470580-41470599 MS.gene89823:CDS 50.0%
GGAGTTGTTCGCGTATTAGG+AGG + chr7.3:41470092-41470111 MS.gene89823:CDS 50.0%
TGTCACCATTGGCATCAAGG+AGG + chr7.3:41470583-41470602 MS.gene89823:CDS 50.0%
TGTTGATCCACACTTGGCAC+CGG + chr7.3:41470208-41470227 MS.gene89823:CDS 50.0%
GGCGGAGTTGTTCGCGTATT+AGG + chr7.3:41470089-41470108 MS.gene89823:CDS 55.0%
! TTGAACTCCGGTGCCAAGTG+TGG - chr7.3:41470218-41470237 None:intergenic 55.0%
ACGCGAACAACTCCGCCCAA+AGG - chr7.3:41470086-41470105 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 41469072 41470803 41469072 ID=MS.gene89823
chr7.3 mRNA 41469072 41470803 41469072 ID=MS.gene89823.t1;Parent=MS.gene89823
chr7.3 exon 41469072 41469746 41469072 ID=MS.gene89823.t1.exon1;Parent=MS.gene89823.t1
chr7.3 CDS 41469072 41469746 41469072 ID=cds.MS.gene89823.t1;Parent=MS.gene89823.t1
chr7.3 exon 41469855 41470604 41469855 ID=MS.gene89823.t1.exon2;Parent=MS.gene89823.t1
chr7.3 CDS 41469855 41470604 41469855 ID=cds.MS.gene89823.t1;Parent=MS.gene89823.t1
chr7.3 exon 41470714 41470803 41470714 ID=MS.gene89823.t1.exon3;Parent=MS.gene89823.t1
chr7.3 CDS 41470714 41470803 41470714 ID=cds.MS.gene89823.t1;Parent=MS.gene89823.t1
Gene Sequence

>MS.gene89823

ATGGCTGCTATTAAAAAAGAGGAGCAAAAGGTTACTATTAAAAAAGAGGAGCAAGTGGCTGCTCCTAATACAGAAGATCAAGTGTTGTTGAAACTTCTGGTGAACAAAGAGACCAACAAAGTCTTAATTGCTGAAGCAGGGAAAGACTTTGTCGATGTTCTATTTAGTTTCTTAACATTGCCATCAGGAACTATTGCAAGACTCATAGAGAAGGACTCAAAGAAGGGACCAGTTACCATTGGTTGTCTGAACAAACTTTATCAAGGTGTGATAGATCTCGATGAAGAGTGTATGCAGACTGAGACAAGCAAAGAAATGTTATTGCACCCAAAGAACTCTTCTGAAGATTATTGCATCAATCTTAAACTTAACATTGATGACATTAAGCCTACAAAGTACTTCATATGTTGCAGGTTTGATTGTCAATGCATACAACAACATAATTTATACACTTCTACAGACAAAAATTGTGTGTATGGAAAAACTTTGAGTCGTTCAACTTTCTTGACACATTTTGGGAAAGGATTTGTTAAAGAAGGTGCAGCTTTTGTTATTACTGATGATCTAATTGTCATGCCGAACTCAGAATTCACAGGGCTAAGCATGCTTCAGAATTCTGGAATAAAAAACACAAGTTCAATAACGGAAATGACTCTCAACGTCACTAAGGAAAAGGTTATTATAAATTTATAGCTGTGTTTCCTAATTTGTTGCTTGCTTTATATACACCGCCTTTTAATTTTTATCATCAATTCATTTGCGATTGATTAATATTTTGTACAGGTACTCGATCTTTTAAAATGTGCATTGCTCTCCAAGTCTACTCTGACAGATTTGTTTCTAAAAAAGAAACCGTTGATCCAGAGGTCAAGTGTTTTCTCTTGTGATGCTGTCATCAAGAAAAATATCAAGATCAGTCTTAAGTTAGTTTTTCGAAAATCAAATGGCAAGATATTGTTTGCTCAAGGAGAAAAAGATTTTGTTGACTTACTTTTAAGTTTTCTCACATTTCCTTTGGGCGGAGTTGTTCGCGTATTAGGAGGGAATTGCTCTTTGGGTAACATTGATTTTCTGTACAAGAGCATAGTTGATTTGCATGCAAGCACATTTTTGACAAAAGAAGCAAAGAAAAGGCTTGTTGATCCACACTTGGCACCGGAGTTCAACAAATTAAGCAAGCAGATATTACCTATTCAAAAGCTACGTGGGCATTATTATTACTATAATAGGTTCCTAGGTTTGGGTAATAGCATTATTCAGAATCAATTTTTCATTAGTGATGAAAATATGAGCAATGAATATGCAGATTATGTGAGAACTGATAGAGAGGTGAACCTAGTTAGTAGTAATCCCAAATCACCTAAGGCAAGTGATAATGGTTATGTAAAAGCAGAGGGACACAGAAAGTATGTGGTTACAGATGATTTGGTCATTGCAACGTCTTCTCCAATTTCAAATCTATTTTTAATCAACAATTTCAAAGTCCCTCTTCATGATGTGAAGGAAAAGCTTGTCACCATTGGCATCAAGGAGGTAAGAAATGACAATTTATACATATATTTGTCACCATGCGTCGTGTTTTTTGGTTTGCTAGATTGATTGAAGATACTATTGCTAATTTTACATAATTGCGTTGTTGCAGTGTCTGAGCATATTAAAGGCATCTTTGTCATCGTCGTTTGCTCTAACAAATGGTCTTCGTCACTTAACCAATGTTAAAGGGGAGAAATGA
Protein sequence

>MS.gene89823.t1

MAAIKKEEQKVTIKKEEQVAAPNTEDQVLLKLLVNKETNKVLIAEAGKDFVDVLFSFLTLPSGTIARLIEKDSKKGPVTIGCLNKLYQGVIDLDEECMQTETSKEMLLHPKNSSEDYCINLKLNIDDIKPTKYFICCRFDCQCIQQHNLYTSTDKNCVYGKTLSRSTFLTHFGKGFVKEGAAFVITDDLIVMPNSEFTGLSMLQNSGIKNTSSITEMTLNVTKEKVLDLLKCALLSKSTLTDLFLKKKPLIQRSSVFSCDAVIKKNIKISLKLVFRKSNGKILFAQGEKDFVDLLLSFLTFPLGGVVRVLGGNCSLGNIDFLYKSIVDLHASTFLTKEAKKRLVDPHLAPEFNKLSKQILPIQKLRGHYYYYNRFLGLGNSIIQNQFFISDENMSNEYADYVRTDREVNLVSSNPKSPKASDNGYVKAEGHRKYVVTDDLVIATSSPISNLFLINNFKVPLHDVKEKLVTIGIKECLSILKASLSSSFALTNGLRHLTNVKGEK