AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50816

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50816.t1 MTR_4g017410 65.878 507 131 7 7 491 4 490 0.0 615
MS.gene50816.t1 MTR_4g017440 65.020 506 150 10 7 491 9 508 0.0 586
MS.gene50816.t1 MTR_4g017830 59.808 520 177 11 1 496 1 512 0.0 554
MS.gene50816.t1 MTR_4g017610 60.941 489 165 9 1 478 1 474 0.0 544
MS.gene50816.t1 MTR_4g017550 64.488 459 136 10 39 478 18 468 0.0 535
MS.gene50816.t1 MTR_4g019340 59.519 499 152 10 1 473 29 503 1.47e-179 513
MS.gene50816.t1 MTR_4g019340 28.571 245 131 7 266 478 41 273 1.53e-11 67.0
MS.gene50816.t1 MTR_4g017470 58.465 508 162 10 1 491 1 476 6.66e-178 509
MS.gene50816.t1 MTR_4g019310 39.293 509 257 13 14 491 10 497 1.68e-94 295
MS.gene50816.t1 MTR_4g019310 26.471 272 152 8 258 494 3 261 2.03e-12 69.7
MS.gene50816.t1 MTR_7g066570 37.449 494 248 17 13 478 15 475 3.97e-71 236
MS.gene50816.t1 MTR_7g058710 32.812 512 270 14 1 478 1 472 3.19e-70 232
MS.gene50816.t1 MTR_7g066430 34.990 503 269 15 13 481 5 483 4.19e-69 229
MS.gene50816.t1 MTR_7g066430 26.316 247 147 6 6 233 251 481 7.84e-12 67.8
MS.gene50816.t1 MTR_7g066350 36.552 435 231 13 13 418 10 428 3.21e-68 226
MS.gene50816.t1 MTR_7g066340 33.667 499 259 14 1 478 1 448 3.61e-68 226
MS.gene50816.t1 MTR_7g066260 34.263 502 244 17 8 478 11 457 9.52e-64 215
MS.gene50816.t1 MTR_7g066410 33.397 521 268 16 13 478 11 507 8.49e-63 213
MS.gene50816.t1 MTR_7g063660 34.980 506 276 18 8 492 5 478 3.60e-62 211
MS.gene50816.t1 MTR_7g063660 35.345 116 59 4 258 359 4 117 7.90e-12 67.8
MS.gene50816.t1 MTR_7g063900 33.192 473 273 12 11 461 9 460 1.05e-60 207
MS.gene50816.t1 MTR_1g041315 32.383 491 242 16 13 467 15 451 6.77e-60 205
MS.gene50816.t1 MTR_7g063880 33.333 507 278 15 2 491 5 468 1.70e-59 203
MS.gene50816.t1 MTR_7g012680 33.205 518 251 18 1 492 4 452 1.26e-56 195
MS.gene50816.t1 MTR_7g066360 35.861 488 239 16 13 481 15 447 4.94e-56 194
MS.gene50816.t1 MTR_7g062280 31.429 525 282 16 8 492 6 492 2.14e-55 193
MS.gene50816.t1 MTR_7g062300 31.881 505 280 16 1 478 1 468 5.45e-55 191
MS.gene50816.t1 MTR_7g066580 32.922 486 241 16 18 471 1 433 1.31e-54 189
MS.gene50816.t1 MTR_7g063840 31.548 504 293 14 7 492 5 474 3.22e-53 186
MS.gene50816.t1 MTR_7g066400 30.675 489 271 12 1 478 1 432 4.59e-53 186
MS.gene50816.t1 MTR_7g063890 32.189 466 274 11 13 457 28 472 1.01e-52 186
MS.gene50816.t1 MTR_7g066290 37.260 365 191 8 8 348 11 361 2.46e-51 181
MS.gene50816.t1 MTR_7g063820 33.977 518 271 22 8 492 6 485 2.85e-51 181
MS.gene50816.t1 MTR_7g066310 30.947 475 215 15 13 478 8 378 4.70e-51 179
MS.gene50816.t1 MTR_7g063830 31.622 487 248 14 13 478 11 433 5.15e-49 174
MS.gene50816.t1 MTR_7g066480 31.070 486 235 12 13 481 17 419 3.27e-47 169
MS.gene50816.t1 MTR_7g066480 27.869 244 126 11 256 476 13 229 9.85e-11 64.3
MS.gene50816.t1 MTR_7g063920 29.980 497 270 13 8 492 7 437 1.56e-46 167
MS.gene50816.t1 MTR_3g065200 30.189 477 256 13 13 477 4 415 2.68e-46 167
MS.gene50816.t1 MTR_2g029400 32.128 470 227 14 13 471 15 403 1.47e-45 164
MS.gene50816.t1 MTR_7g063670 29.941 511 272 16 5 492 11 458 2.30e-42 157
MS.gene50816.t1 MTR_7g058700 33.146 356 153 10 22 339 529 837 6.39e-41 157
MS.gene50816.t1 MTR_4g019240 62.295 122 41 3 4 125 5 121 7.26e-41 145
MS.gene50816.t1 MTR_4g019240 65.455 55 17 1 441 495 112 164 3.09e-11 62.8
MS.gene50816.t1 MTR_3g007740 28.776 490 273 16 8 478 5 437 1.59e-39 149
MS.gene50816.t1 MTR_7g066490 27.984 486 236 13 13 481 17 405 4.54e-36 139
MS.gene50816.t1 MTR_7g066280 28.883 412 231 12 102 478 1 385 8.23e-32 127
MS.gene50816.t1 MTR_7g066680 32.222 270 144 9 214 463 37 287 1.28e-26 110
MS.gene50816.t1 MTR_1g014060 33.906 233 132 9 21 243 9 229 9.42e-24 103
MS.gene50816.t1 MTR_1g014060 31.795 195 99 4 293 478 210 379 1.15e-13 73.2
MS.gene50816.t1 MTR_7g062270 35.870 184 95 7 7 180 5 175 1.48e-21 97.1
MS.gene50816.t1 MTR_8g445860 28.862 246 120 7 263 478 13 233 2.90e-19 87.4
MS.gene50816.t1 MTR_8g445860 29.046 241 139 6 10 234 11 235 9.60e-16 77.0
MS.gene50816.t1 MTR_7g100420 31.429 280 156 10 214 478 134 392 8.80e-18 85.9
MS.gene50816.t1 MTR_7g100420 49.206 63 32 0 12 74 39 101 8.68e-13 70.5
MS.gene50816.t1 MTR_1g115440 28.279 244 134 7 13 234 12 236 2.29e-17 82.0
MS.gene50816.t1 MTR_7g063720 29.167 240 132 5 253 480 10 223 4.21e-17 81.3
MS.gene50816.t1 MTR_0150s0010 27.386 241 140 5 13 234 11 235 3.61e-15 75.5
MS.gene50816.t1 MTR_0150s0010 27.897 233 152 7 258 478 5 233 1.62e-13 70.5
MS.gene50816.t1 MTR_7g063690 28.521 284 135 9 7 290 7 222 1.69e-14 73.6
MS.gene50816.t1 MTR_1g115455 26.749 243 135 7 13 234 10 230 1.99e-14 73.2
MS.gene50816.t1 MTR_6g006800 74.468 47 11 1 1 47 1 46 5.48e-14 67.4
MS.gene50816.t1 MTR_8g023240 21.850 508 304 16 5 475 30 481 5.89e-12 68.6
MS.gene50816.t1 MTR_1g115450 40.506 79 39 1 263 333 7 85 2.50e-11 62.0
MS.gene50816.t1 MTR_1g115450 33.884 121 62 3 10 129 5 108 4.77e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50816.t1 AT5G01130 31.313 495 271 15 13 491 10 451 6.73e-55 191
MS.gene50816.t1 AT5G01120 30.815 503 283 17 13 478 10 484 3.05e-53 187
MS.gene50816.t1 AT5G01140 30.830 506 262 17 15 491 12 458 2.75e-48 173
MS.gene50816.t1 AT5G43240 30.561 517 270 21 13 478 10 488 3.09e-48 174
MS.gene50816.t1 AT5G43240 30.561 517 270 21 13 478 10 488 3.09e-48 174
MS.gene50816.t1 AT5G43240 30.561 517 270 21 13 478 10 488 3.09e-48 174
MS.gene50816.t1 AT3G09140 30.620 516 277 17 10 478 7 488 7.85e-48 172
MS.gene50816.t1 AT5G01150 30.123 488 292 12 15 478 12 474 5.07e-45 165
MS.gene50816.t1 AT3G09110 32.448 339 201 10 15 339 12 336 2.14e-43 157
MS.gene50816.t1 AT3G09140 29.044 544 277 18 10 478 7 516 1.30e-42 159
MS.gene50816.t1 AT3G09120 34.146 328 178 10 10 317 4 313 5.17e-42 152
MS.gene50816.t1 AT5G37320 29.515 515 289 19 7 485 3 479 4.39e-39 148
MS.gene50816.t1 AT5G43240 28.446 341 193 13 166 478 27 344 4.07e-22 98.2

Find 100 sgRNAs with CRISPR-Local

Find 172 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GACCCTCATAATCCAAATTT+TGG 0.074085 4.1:-80048789 None:intergenic
AGGTGGGTTGTCTTTATTTA+TGG 0.113150 4.1:-80049828 None:intergenic
GCCTCTCACAAATACAATTT+TGG 0.193780 4.1:+80049796 MS.gene50816:CDS
AAACGGAGAGGCCCAAAATT+TGG 0.202521 4.1:+80048777 MS.gene50816:CDS
CACCAATAACAGAAATAAAA+TGG 0.256157 4.1:+80049886 MS.gene50816:CDS
ACCAGATGCATCAATGTTTC+TGG 0.258927 4.1:+80048816 MS.gene50816:CDS
AGGTTCCATTGTAAACCTTT+TGG 0.300527 4.1:+80050009 MS.gene50816:CDS
TTGATCATTGCTGTTGTTGA+TGG 0.321006 4.1:-80048485 None:intergenic
TTTAAGCTGTTGATGGAGTT+GGG 0.330345 4.1:+80048883 MS.gene50816:CDS
CTTTAAGCTGTTGATGGAGT+TGG 0.330640 4.1:+80048882 MS.gene50816:CDS
GTGAAGAGAGAAGGATTATA+TGG 0.333879 4.1:+80050352 MS.gene50816:CDS
CAGTGATTCACATGGATTCT+TGG 0.338174 4.1:-80048635 None:intergenic
CTGGTCCACTGGCACTAGTC+AGG 0.353124 4.1:-80050620 None:intergenic
ACACCTTCTTCTGACCTGTT+GGG 0.354032 4.1:-80050319 None:intergenic
GACTTACTTTAAGCTGTTGA+TGG 0.354471 4.1:+80048876 MS.gene50816:CDS
TGTTGTTGATGGTGGCTAAA+AGG 0.368114 4.1:-80048474 None:intergenic
GCAACATTTGTTTGCAGAAA+GGG 0.373252 4.1:-80048606 None:intergenic
CACCTTCTTCTGACCTGTTG+GGG 0.390319 4.1:-80050318 None:intergenic
CACACCTTCTTCTGACCTGT+TGG 0.394282 4.1:-80050320 None:intergenic
GGCCCAAAATTTGGATTATG+AGG 0.396371 4.1:+80048786 MS.gene50816:CDS
AAACATCATCGTCTGAGTTA+AGG 0.396737 4.1:-80048576 None:intergenic
TTAACTCAGACGATGATGTT+TGG 0.397225 4.1:+80048578 MS.gene50816:CDS
GGAATGTTGAGGGGTTGGTT+TGG 0.398404 4.1:-80050139 None:intergenic
AGCTTCCTTACCATGCCATT+AGG 0.400764 4.1:+80049989 MS.gene50816:CDS
AATGGATCTCTCTTTCGCTT+TGG 0.418129 4.1:-80050202 None:intergenic
AGTGATTCACATGGATTCTT+GGG 0.421877 4.1:-80048634 None:intergenic
ACACATCTAGCAAGAGAGTT+AGG 0.425012 4.1:-80050034 None:intergenic
CATAAGGAATGTTGAGGGGT+TGG 0.425340 4.1:-80050144 None:intergenic
GGCACTTTCAAAAGGTCTCC+TGG 0.431676 4.1:-80050639 None:intergenic
AAAACCTTCATTGCGCTTGT+GGG 0.435042 4.1:+80048746 MS.gene50816:CDS
GAGAGGCTCATTAGAGGTTA+AGG 0.435850 4.1:-80049780 None:intergenic
ATTTGTGAGAGGCTCATTAG+AGG 0.437734 4.1:-80049786 None:intergenic
GATTCCCCTCAAAATCTTAG+TGG 0.437735 4.1:+80048354 MS.gene50816:CDS
ACCAGAAACATTGATGCATC+TGG 0.438385 4.1:-80048817 None:intergenic
CAAAACCTTCATTGCGCTTG+TGG 0.445825 4.1:+80048745 MS.gene50816:CDS
CAAAAGGTCTCCTGGTCCAC+TGG 0.449938 4.1:-80050631 None:intergenic
GTACAAGAGTGTGGAGAACT+TGG 0.454304 4.1:+80050066 MS.gene50816:CDS
CTTGTTTAGGCACTTTCAAA+AGG 0.456406 4.1:-80050647 None:intergenic
CAAGAGAGTTAGGTCCCAAA+AGG 0.465979 4.1:-80050024 None:intergenic
AGATTCTGATCGACGTGTCT+TGG 0.469015 4.1:-80050269 None:intergenic
ACTGTTGGTGCAGATTGCTT+AGG 0.472248 4.1:-80049848 None:intergenic
AGGTTAAGGAATACTTGAGC+AGG 0.484909 4.1:-80049766 None:intergenic
CCTTCTTCTGACCTGTTGGG+GGG 0.488088 4.1:-80050316 None:intergenic
GTTTCCCACAAGCGCAATGA+AGG 0.491621 4.1:-80048750 None:intergenic
AGCAACATTTGTTTGCAGAA+AGG 0.493897 4.1:-80048607 None:intergenic
ATCATTGCTGTTGTTGATGG+TGG 0.500624 4.1:-80048482 None:intergenic
AGGTTTACAATGGAACCTAA+TGG 0.501112 4.1:-80050004 None:intergenic
GGATTTAGTAACACAGACTT+GGG 0.501573 4.1:-80050091 None:intergenic
CTGACTAGTGCCAGTGGACC+AGG 0.505141 4.1:+80050621 MS.gene50816:CDS
AACTCTCTTGCTAGATGTGT+TGG 0.507764 4.1:+80050037 MS.gene50816:CDS
GTTGGTGCAGATTGCTTAGG+TGG 0.508685 4.1:-80049845 None:intergenic
AGAGAAGGATTATATGGTGT+AGG 0.509059 4.1:+80050358 MS.gene50816:CDS
AAGTCTGTGTTACTAAATCC+TGG 0.510892 4.1:+80050094 MS.gene50816:CDS
CACACACAACATTGGCAAGC+AGG 0.511161 4.1:+80048936 MS.gene50816:CDS
CAAAACTGTGGTGCTATACC+AGG 0.511808 4.1:-80050112 None:intergenic
ACCTTCTTCTGACCTGTTGG+GGG 0.519536 4.1:-80050317 None:intergenic
AGACGCAAATAAACTGAAAG+TGG 0.521334 4.1:-80050400 None:intergenic
TCTTGACGATCTTGAAGTGA+AGG 0.522916 4.1:+80050450 MS.gene50816:CDS
TTATTTCTGTTATTGGTGCA+AGG 0.532286 4.1:-80049881 None:intergenic
CTTCTTCTGACCTGTTGGGG+GGG 0.534388 4.1:-80050315 None:intergenic
AGGATTTAGTAACACAGACT+TGG 0.536535 4.1:-80050092 None:intergenic
CTTCAAGATCGTCAAGAGGA+AGG 0.538256 4.1:-80050444 None:intergenic
CAACATTTGTTTGCAGAAAG+GGG 0.540436 4.1:-80048605 None:intergenic
GCCAAAATTGTATTTGTGAG+AGG 0.542965 4.1:-80049797 None:intergenic
TTAAGCTGTTGATGGAGTTG+GGG 0.553198 4.1:+80048884 MS.gene50816:CDS
TGGTTGCAAGTGGAAGAGAT+AGG 0.556941 4.1:-80048444 None:intergenic
GAAGTGAAGGAAATAAGCAT+TGG 0.567701 4.1:+80050463 MS.gene50816:CDS
GAAGTCAACAAAATCTCCGT+TGG 0.575489 4.1:-80049960 None:intergenic
TGGAACCTAATGGCATGGTA+AGG 0.579585 4.1:-80049994 None:intergenic
TTGGTGCAGATTGCTTAGGT+GGG 0.579593 4.1:-80049844 None:intergenic
TACAATGGAACCTAATGGCA+TGG 0.581094 4.1:-80049999 None:intergenic
AGGTCCCAAAAGGTTTACAA+TGG 0.585270 4.1:-80050014 None:intergenic
AGGTCAGAAGAAGGTGTGGT+GGG 0.585287 4.1:+80050325 MS.gene50816:CDS
GGTGTTCGCAGAAGCAAACA+AGG 0.590592 4.1:+80048396 MS.gene50816:CDS
TGGTCCACTGGCACTAGTCA+GGG 0.594688 4.1:-80050619 None:intergenic
GCCCAAAATTTGGATTATGA+GGG 0.595994 4.1:+80048787 MS.gene50816:CDS
TTCACTTCAAGATCGTCAAG+AGG 0.596428 4.1:-80050448 None:intergenic
CAGGTCAGAAGAAGGTGTGG+TGG 0.596620 4.1:+80050324 MS.gene50816:CDS
GCTTGTGGGAAACCAACAAA+CGG 0.604091 4.1:+80048760 MS.gene50816:CDS
AATAAGCATTGGTGAAGCTG+AGG 0.609065 4.1:+80050474 MS.gene50816:CDS
GTGGGATTTGTGAAGAGAGA+AGG 0.609295 4.1:+80050343 MS.gene50816:CDS
CAAGGCAATACTCTAACTGT+TGG 0.616222 4.1:-80049863 None:intergenic
GAGGAAGTCACACACAACAT+TGG 0.621785 4.1:+80048928 MS.gene50816:CDS
AGGCGAGCAATGGTTGCAAG+TGG 0.626437 4.1:-80048454 None:intergenic
ATGATATTCACAGAAGCCAA+CGG 0.635551 4.1:+80049944 MS.gene50816:CDS
TTCAGACTTGACTCAACTAG+AGG 0.639443 4.1:+80048909 MS.gene50816:CDS
TTTACATAAGGAATGTTGAG+GGG 0.645423 4.1:-80050148 None:intergenic
CAACAGGTCAGAAGAAGGTG+TGG 0.646980 4.1:+80050321 MS.gene50816:CDS
TTGATGTCAAGAATGCTCGA+AGG 0.662606 4.1:-80050590 None:intergenic
TGGTAACTTGTACAAGAGTG+TGG 0.668210 4.1:+80050057 MS.gene50816:CDS
GGACAAACAAAGCAACAAAG+TGG 0.672252 4.1:+80048375 MS.gene50816:CDS
GGTGGCTAAAAGGCGAGCAA+TGG 0.674121 4.1:-80048464 None:intergenic
ACACAACATTGGCAAGCAGG+AGG 0.678135 4.1:+80048939 MS.gene50816:CDS
TTTACCCTGACTAGTGCCAG+TGG 0.682350 4.1:+80050615 MS.gene50816:CDS
CAGCTTAAAGTAAGTCACAG+AGG 0.704820 4.1:-80048869 None:intergenic
TTTATGCACAGTGATTCACA+TGG 0.706252 4.1:-80048643 None:intergenic
TGGGAAACCAACAAACGGAG+AGG 0.713749 4.1:+80048765 MS.gene50816:CDS
TGGTTTGGACAACAAAACTG+TGG 0.726414 4.1:-80050124 None:intergenic
GATTTAGTAACACAGACTTG+GGG 0.736630 4.1:-80050090 None:intergenic
TATAATAGAAGAATTCACTG+AGG 0.766608 4.1:+80050234 MS.gene50816:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATTTAATATTCACAATCTT+AGG + chr4.1:80049338-80049357 MS.gene50816:intron 15.0%
!! TATTATTCTGTTACAAAAAA+AGG - chr4.1:80049494-80049513 None:intergenic 15.0%
!! TCTATTATATGAAAAATGAA+TGG - chr4.1:80050223-80050242 None:intergenic 15.0%
!!! ATTTAAGATCTATTGAATTT+TGG - chr4.1:80049289-80049308 None:intergenic 15.0%
!!! TTTAAGATCTATTGAATTTT+GGG - chr4.1:80049288-80049307 None:intergenic 15.0%
!!! TTTTAGCATAATAATAAGTA+GGG - chr4.1:80050177-80050196 None:intergenic 15.0%
!! CAAACAAAAAACTTATTTGA+TGG - chr4.1:80048697-80048716 None:intergenic 20.0%
!! CCTCAAAATTTATTATATTG+TGG + chr4.1:80049181-80049200 MS.gene50816:intron 20.0%
!! TCAATATCTATACATCAAAA+AGG - chr4.1:80049745-80049764 None:intergenic 20.0%
!! TTGATTGAAAGAAATATAGA+TGG - chr4.1:80049658-80049677 None:intergenic 20.0%
!!! ATCCATTTTATTTCTGTTAT+TGG - chr4.1:80049891-80049910 None:intergenic 20.0%
!!! CCACAATATAATAAATTTTG+AGG - chr4.1:80049184-80049203 None:intergenic 20.0%
!!! CTTTTAGCATAATAATAAGT+AGG - chr4.1:80050178-80050197 None:intergenic 20.0%
!!! GTAAAATTTTGTTCTTGTTT+AGG - chr4.1:80050663-80050682 None:intergenic 20.0%
!!! TCTATATTTTACCACAATAA+AGG - chr4.1:80049120-80049139 None:intergenic 20.0%
!!! TTAAGATCTATTGAATTTTG+GGG - chr4.1:80049287-80049306 None:intergenic 20.0%
! ATAAAGAACAATGTAATGAG+AGG - chr4.1:80049020-80049039 None:intergenic 25.0%
! ATTTCTTCTAACATGTCTTA+AGG - chr4.1:80049522-80049541 None:intergenic 25.0%
! CACCAATAACAGAAATAAAA+TGG + chr4.1:80049886-80049905 MS.gene50816:CDS 25.0%
! TAAAGAACAATGTAATGAGA+GGG - chr4.1:80049019-80049038 None:intergenic 25.0%
! TATAATAGAAGAATTCACTG+AGG + chr4.1:80050234-80050253 MS.gene50816:CDS 25.0%
! TTAGAGAAGTTATTAGTTAG+AGG + chr4.1:80049458-80049477 MS.gene50816:intron 25.0%
! TTGAAAGAACTTGAAATCTT+TGG + chr4.1:80049580-80049599 MS.gene50816:intron 25.0%
! TTTCTTCTAACATGTCTTAA+GGG - chr4.1:80049521-80049540 None:intergenic 25.0%
!! AATTTCGAATTTGAAACACA+AGG + chr4.1:80049306-80049325 MS.gene50816:intron 25.0%
!! AGGAAATTATCAATTGCTTT+TGG - chr4.1:80049725-80049744 None:intergenic 25.0%
!! TATTTTACCACAATAAAGGT+AGG - chr4.1:80049116-80049135 None:intergenic 25.0%
!! TGTTCTTTATTTACTTGCAT+AGG + chr4.1:80049030-80049049 MS.gene50816:intron 25.0%
!! TTTTACATAAGGAATGTTGA+GGG - chr4.1:80050152-80050171 None:intergenic 25.0%
!!! GTTTTTGAATGTTGTTGTTT+TGG - chr4.1:80048334-80048353 None:intergenic 25.0%
!!! TCAATCAATTTTTCAATCCT+TGG + chr4.1:80049670-80049689 MS.gene50816:intron 25.0%
!!! TTAGTATGTCTTCTTGTTTT+AGG - chr4.1:80049422-80049441 None:intergenic 25.0%
AAAGAACTTGAAATCTTTGG+AGG + chr4.1:80049583-80049602 MS.gene50816:intron 30.0%
AACCAAAAATTAAAACCACG+AGG - chr4.1:80049087-80049106 None:intergenic 30.0%
AGTAGTACTATGTTTCCTAA+AGG - chr4.1:80049229-80049248 None:intergenic 30.0%
ATTTATTATATTGTGGCTGC+AGG + chr4.1:80049188-80049207 MS.gene50816:intron 30.0%
GTAGTACTATGTTTCCTAAA+GGG - chr4.1:80049228-80049247 None:intergenic 30.0%
TTAGGAAACATAGTACTACT+AGG + chr4.1:80049229-80049248 MS.gene50816:intron 30.0%
TTTACATAAGGAATGTTGAG+GGG - chr4.1:80050151-80050170 None:intergenic 30.0%
! ACCCTCATAATCCAAATTTT+GGG - chr4.1:80048791-80048810 None:intergenic 30.0%
! GTTTTACATAAGGAATGTTG+AGG - chr4.1:80050153-80050172 None:intergenic 30.0%
! TTTGTCCACTAAGATTTTGA+GGG - chr4.1:80048362-80048381 None:intergenic 30.0%
!! CTTTTGATGTTTTCAAGGAA+TGG - chr4.1:80048535-80048554 None:intergenic 30.0%
!! TAAGTAGGGTGTTTTACATA+AGG - chr4.1:80050163-80050182 None:intergenic 30.0%
!! TTATTTCTGTTATTGGTGCA+AGG - chr4.1:80049884-80049903 None:intergenic 30.0%
AAACATCATCGTCTGAGTTA+AGG - chr4.1:80048579-80048598 None:intergenic 35.0%
AACAATGTAATGAGAGGGTA+TGG - chr4.1:80049014-80049033 None:intergenic 35.0%
ACAACATTAAGAGTTAGCCA+AGG - chr4.1:80049690-80049709 None:intergenic 35.0%
AGACGCAAATAAACTGAAAG+TGG - chr4.1:80050403-80050422 None:intergenic 35.0%
AGAGAAGGATTATATGGTGT+AGG + chr4.1:80050358-80050377 MS.gene50816:CDS 35.0%
AGCAACATTTGTTTGCAGAA+AGG - chr4.1:80048610-80048629 None:intergenic 35.0%
AGGATTTAGTAACACAGACT+TGG - chr4.1:80050095-80050114 None:intergenic 35.0%
AGGTTTACAATGGAACCTAA+TGG - chr4.1:80050007-80050026 None:intergenic 35.0%
AGTGATTCACATGGATTCTT+GGG - chr4.1:80048637-80048656 None:intergenic 35.0%
ATGATATTCACAGAAGCCAA+CGG + chr4.1:80049944-80049963 MS.gene50816:CDS 35.0%
CAAAAATTAAAACCACGAGG+TGG - chr4.1:80049084-80049103 None:intergenic 35.0%
CAACATTTGTTTGCAGAAAG+GGG - chr4.1:80048608-80048627 None:intergenic 35.0%
CTTGTTTAGGCACTTTCAAA+AGG - chr4.1:80050650-80050669 None:intergenic 35.0%
GAAGTGAAGGAAATAAGCAT+TGG + chr4.1:80050463-80050482 MS.gene50816:CDS 35.0%
GACTTACTTTAAGCTGTTGA+TGG + chr4.1:80048876-80048895 MS.gene50816:CDS 35.0%
GAGAAGTTATTAGTTAGAGG+AGG + chr4.1:80049461-80049480 MS.gene50816:intron 35.0%
GATTTAGTAACACAGACTTG+GGG - chr4.1:80050093-80050112 None:intergenic 35.0%
GCAACATTTGTTTGCAGAAA+GGG - chr4.1:80048609-80048628 None:intergenic 35.0%
GCCAAAATTGTATTTGTGAG+AGG - chr4.1:80049800-80049819 None:intergenic 35.0%
GCCCAAAATTTGGATTATGA+GGG + chr4.1:80048787-80048806 MS.gene50816:CDS 35.0%
GGATTTAGTAACACAGACTT+GGG - chr4.1:80050094-80050113 None:intergenic 35.0%
GTGAAGAGAGAAGGATTATA+TGG + chr4.1:80050352-80050371 MS.gene50816:CDS 35.0%
TGGAGGCTAATGATTAAGAT+TGG - chr4.1:80049064-80049083 None:intergenic 35.0%
TTAACTCAGACGATGATGTT+TGG + chr4.1:80048578-80048597 MS.gene50816:CDS 35.0%
TTTATGCACAGTGATTCACA+TGG - chr4.1:80048646-80048665 None:intergenic 35.0%
! AAGTCTGTGTTACTAAATCC+TGG + chr4.1:80050094-80050113 MS.gene50816:CDS 35.0%
! ACAATGTAATGAGAGGGTAT+GGG - chr4.1:80049013-80049032 None:intergenic 35.0%
! AGAGGCTTTTGATGTTTTCA+AGG - chr4.1:80048540-80048559 None:intergenic 35.0%
! AGGTTCCATTGTAAACCTTT+TGG + chr4.1:80050009-80050028 MS.gene50816:CDS 35.0%
! AGTTAAGGTTTTCGACAGTT+TGG - chr4.1:80048564-80048583 None:intergenic 35.0%
! GACCCTCATAATCCAAATTT+TGG - chr4.1:80048792-80048811 None:intergenic 35.0%
! GCCTCTCACAAATACAATTT+TGG + chr4.1:80049796-80049815 MS.gene50816:CDS 35.0%
! GGTTCCATTGTAAACCTTTT+GGG + chr4.1:80050010-80050029 MS.gene50816:CDS 35.0%
! GTTTGTCCACTAAGATTTTG+AGG - chr4.1:80048363-80048382 None:intergenic 35.0%
! TTGATCATTGCTGTTGTTGA+TGG - chr4.1:80048488-80048507 None:intergenic 35.0%
! TTGTCCACTAAGATTTTGAG+GGG - chr4.1:80048361-80048380 None:intergenic 35.0%
! TTTAAGCTGTTGATGGAGTT+GGG + chr4.1:80048883-80048902 MS.gene50816:CDS 35.0%
! TTTTCTGCCTACCTTTATTG+TGG + chr4.1:80049106-80049125 MS.gene50816:intron 35.0%
!! AGGTGGGTTGTCTTTATTTA+TGG - chr4.1:80049831-80049850 None:intergenic 35.0%
!!! CACCTCGTGGTTTTAATTTT+TGG + chr4.1:80049082-80049101 MS.gene50816:intron 35.0%
AAAACCTTCATTGCGCTTGT+GGG + chr4.1:80048746-80048765 MS.gene50816:CDS 40.0%
AAATTAAAACCACGAGGTGG+AGG - chr4.1:80049081-80049100 None:intergenic 40.0%
AACTCTCTTGCTAGATGTGT+TGG + chr4.1:80050037-80050056 MS.gene50816:CDS 40.0%
AATGGATCTCTCTTTCGCTT+TGG - chr4.1:80050205-80050224 None:intergenic 40.0%
ACACATCTAGCAAGAGAGTT+AGG - chr4.1:80050037-80050056 None:intergenic 40.0%
ACCAGAAACATTGATGCATC+TGG - chr4.1:80048820-80048839 None:intergenic 40.0%
ACCAGATGCATCAATGTTTC+TGG + chr4.1:80048816-80048835 MS.gene50816:CDS 40.0%
AGGTCCCAAAAGGTTTACAA+TGG - chr4.1:80050017-80050036 None:intergenic 40.0%
AGGTTAAGGAATACTTGAGC+AGG - chr4.1:80049769-80049788 None:intergenic 40.0%
ATTTGACGTTGCGTATCAGT+TGG + chr4.1:80049252-80049271 MS.gene50816:intron 40.0%
ATTTGTGAGAGGCTCATTAG+AGG - chr4.1:80049789-80049808 None:intergenic 40.0%
CAAGGCAATACTCTAACTGT+TGG - chr4.1:80049866-80049885 None:intergenic 40.0%
CAGCTTAAAGTAAGTCACAG+AGG - chr4.1:80048872-80048891 None:intergenic 40.0%
CAGGAGGTAATGATATTGTG+TGG + chr4.1:80048955-80048974 MS.gene50816:intron 40.0%
CAGTGATTCACATGGATTCT+TGG - chr4.1:80048638-80048657 None:intergenic 40.0%
CTACATCATAACCGAAGTTG+TGG - chr4.1:80049152-80049171 None:intergenic 40.0%
CTTCGGTTATGATGTAGTTG+TGG + chr4.1:80049155-80049174 MS.gene50816:intron 40.0%
GAAGTCAACAAAATCTCCGT+TGG - chr4.1:80049963-80049982 None:intergenic 40.0%
GATTCCCCTCAAAATCTTAG+TGG + chr4.1:80048354-80048373 MS.gene50816:CDS 40.0%
GGACAAACAAAGCAACAAAG+TGG + chr4.1:80048375-80048394 MS.gene50816:CDS 40.0%
GGCCCAAAATTTGGATTATG+AGG + chr4.1:80048786-80048805 MS.gene50816:CDS 40.0%
TACAATGGAACCTAATGGCA+TGG - chr4.1:80050002-80050021 None:intergenic 40.0%
TCTTGACGATCTTGAAGTGA+AGG + chr4.1:80050450-80050469 MS.gene50816:CDS 40.0%
TTCACTTCAAGATCGTCAAG+AGG - chr4.1:80050451-80050470 None:intergenic 40.0%
TTCAGACTTGACTCAACTAG+AGG + chr4.1:80048909-80048928 MS.gene50816:CDS 40.0%
TTGATGTCAAGAATGCTCGA+AGG - chr4.1:80050593-80050612 None:intergenic 40.0%
! ATCATTGCTGTTGTTGATGG+TGG - chr4.1:80048485-80048504 None:intergenic 40.0%
! CTTTAAGCTGTTGATGGAGT+TGG + chr4.1:80048882-80048901 MS.gene50816:CDS 40.0%
! TGGTAACTTGTACAAGAGTG+TGG + chr4.1:80050057-80050076 MS.gene50816:CDS 40.0%
! TGGTTTGGACAACAAAACTG+TGG - chr4.1:80050127-80050146 None:intergenic 40.0%
! TTAAGCTGTTGATGGAGTTG+GGG + chr4.1:80048884-80048903 MS.gene50816:CDS 40.0%
!! AATAAGCATTGGTGAAGCTG+AGG + chr4.1:80050474-80050493 MS.gene50816:CDS 40.0%
!! TGTTGTTGATGGTGGCTAAA+AGG - chr4.1:80048477-80048496 None:intergenic 40.0%
AAACGGAGAGGCCCAAAATT+TGG + chr4.1:80048777-80048796 MS.gene50816:CDS 45.0%
ACACCTTCTTCTGACCTGTT+GGG - chr4.1:80050322-80050341 None:intergenic 45.0%
ACTGTTGGTGCAGATTGCTT+AGG - chr4.1:80049851-80049870 None:intergenic 45.0%
AGATTCTGATCGACGTGTCT+TGG - chr4.1:80050272-80050291 None:intergenic 45.0%
AGCTTCCTTACCATGCCATT+AGG + chr4.1:80049989-80050008 MS.gene50816:CDS 45.0%
CAAAACCTTCATTGCGCTTG+TGG + chr4.1:80048745-80048764 MS.gene50816:CDS 45.0%
CAAGAGAGTTAGGTCCCAAA+AGG - chr4.1:80050027-80050046 None:intergenic 45.0%
CATAAGGAATGTTGAGGGGT+TGG - chr4.1:80050147-80050166 None:intergenic 45.0%
CATCATAACCGAAGTTGTGG+CGG - chr4.1:80049149-80049168 None:intergenic 45.0%
CTTCAAGATCGTCAAGAGGA+AGG - chr4.1:80050447-80050466 None:intergenic 45.0%
GAGAGGCTCATTAGAGGTTA+AGG - chr4.1:80049783-80049802 None:intergenic 45.0%
GAGGAAGTCACACACAACAT+TGG + chr4.1:80048928-80048947 MS.gene50816:CDS 45.0%
GATAAATGCCGCCACAACTT+CGG + chr4.1:80049138-80049157 MS.gene50816:intron 45.0%
GCTTGTGGGAAACCAACAAA+CGG + chr4.1:80048760-80048779 MS.gene50816:CDS 45.0%
GTGGGATTTGTGAAGAGAGA+AGG + chr4.1:80050343-80050362 MS.gene50816:CDS 45.0%
TGGAACCTAATGGCATGGTA+AGG - chr4.1:80049997-80050016 None:intergenic 45.0%
TGGTTGCAAGTGGAAGAGAT+AGG - chr4.1:80048447-80048466 None:intergenic 45.0%
TTATATTGTGGCTGCAGGTG+TGG + chr4.1:80049193-80049212 MS.gene50816:intron 45.0%
TTCCTCTTGACTTCTGATGC+AGG + chr4.1:80050557-80050576 MS.gene50816:intron 45.0%
TTGGTGCAGATTGCTTAGGT+GGG - chr4.1:80049847-80049866 None:intergenic 45.0%
! GGTTTTCGACAGTTTGGTAG+AGG - chr4.1:80048558-80048577 None:intergenic 45.0%
! GTACAAGAGTGTGGAGAACT+TGG + chr4.1:80050066-80050085 MS.gene50816:CDS 45.0%
! GTTGCGTATCAGTTGGTGTT+AGG + chr4.1:80049259-80049278 MS.gene50816:intron 45.0%
! TGTGGCACGTTTTACCCTTT+AGG + chr4.1:80049211-80049230 MS.gene50816:intron 45.0%
!! CAAAACTGTGGTGCTATACC+AGG - chr4.1:80050115-80050134 None:intergenic 45.0%
ACACAACATTGGCAAGCAGG+AGG + chr4.1:80048939-80048958 MS.gene50816:CDS 50.0%
ACCTTCTTCTGACCTGTTGG+GGG - chr4.1:80050320-80050339 None:intergenic 50.0%
AGCCTGCATCAGAAGTCAAG+AGG - chr4.1:80050562-80050581 None:intergenic 50.0%
CAACAGGTCAGAAGAAGGTG+TGG + chr4.1:80050321-80050340 MS.gene50816:CDS 50.0%
CACACACAACATTGGCAAGC+AGG + chr4.1:80048936-80048955 MS.gene50816:CDS 50.0%
CACACCTTCTTCTGACCTGT+TGG - chr4.1:80050323-80050342 None:intergenic 50.0%
CACCTTCTTCTGACCTGTTG+GGG - chr4.1:80050321-80050340 None:intergenic 50.0%
GGCACTTTCAAAAGGTCTCC+TGG - chr4.1:80050642-80050661 None:intergenic 50.0%
GGTGTTCGCAGAAGCAAACA+AGG + chr4.1:80048396-80048415 MS.gene50816:CDS 50.0%
GTTGGTGCAGATTGCTTAGG+TGG - chr4.1:80049848-80049867 None:intergenic 50.0%
GTTTCCCACAAGCGCAATGA+AGG - chr4.1:80048753-80048772 None:intergenic 50.0%
TGGGAAACCAACAAACGGAG+AGG + chr4.1:80048765-80048784 MS.gene50816:CDS 50.0%
TTTACCCTGACTAGTGCCAG+TGG + chr4.1:80050615-80050634 MS.gene50816:CDS 50.0%
! AATCATTAGCCTCCACCTCG+TGG + chr4.1:80049069-80049088 MS.gene50816:intron 50.0%
! AGGTCAGAAGAAGGTGTGGT+GGG + chr4.1:80050325-80050344 MS.gene50816:CDS 50.0%
! GCATTGGTGAAGCTGAGGTA+AGG + chr4.1:80050479-80050498 MS.gene50816:intron 50.0%
! TTTTGGGCCTCTCCGTTTGT+TGG - chr4.1:80048775-80048794 None:intergenic 50.0%
!! GGAATGTTGAGGGGTTGGTT+TGG - chr4.1:80050142-80050161 None:intergenic 50.0%
AGGCGAGCAATGGTTGCAAG+TGG - chr4.1:80048457-80048476 None:intergenic 55.0%
CAAAAGGTCTCCTGGTCCAC+TGG - chr4.1:80050634-80050653 None:intergenic 55.0%
CCTTCTTCTGACCTGTTGGG+GGG - chr4.1:80050319-80050338 None:intergenic 55.0%
CTTCTTCTGACCTGTTGGGG+GGG - chr4.1:80050318-80050337 None:intergenic 55.0%
GGTGGCTAAAAGGCGAGCAA+TGG - chr4.1:80048467-80048486 None:intergenic 55.0%
TGGTCCACTGGCACTAGTCA+GGG - chr4.1:80050622-80050641 None:intergenic 55.0%
! CAGGTCAGAAGAAGGTGTGG+TGG + chr4.1:80050324-80050343 MS.gene50816:CDS 55.0%
CCCCCCAACAGGTCAGAAGA+AGG + chr4.1:80050316-80050335 MS.gene50816:CDS 60.0%
CTGACTAGTGCCAGTGGACC+AGG + chr4.1:80050621-80050640 MS.gene50816:CDS 60.0%
CTGGTCCACTGGCACTAGTC+AGG - chr4.1:80050623-80050642 None:intergenic 60.0%
CTGCACTCGACCCCCCCAAC+AGG + chr4.1:80050305-80050324 MS.gene50816:CDS 70.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80048319 80050689 80048319 ID=MS.gene50816
chr4.1 mRNA 80048319 80050689 80048319 ID=MS.gene50816.t1;Parent=MS.gene50816
chr4.1 exon 80048319 80048960 80048319 ID=MS.gene50816.t1.exon1;Parent=MS.gene50816.t1
chr4.1 CDS 80048319 80048960 80048319 ID=cds.MS.gene50816.t1;Parent=MS.gene50816.t1
chr4.1 exon 80049758 80050495 80049758 ID=MS.gene50816.t1.exon2;Parent=MS.gene50816.t1
chr4.1 CDS 80049758 80050495 80049758 ID=cds.MS.gene50816.t1;Parent=MS.gene50816.t1
chr4.1 exon 80050579 80050689 80050579 ID=MS.gene50816.t1.exon3;Parent=MS.gene50816.t1
chr4.1 CDS 80050579 80050689 80050579 ID=cds.MS.gene50816.t1;Parent=MS.gene50816.t1
Gene Sequence

>MS.gene50816

ATGACTTCTCAACCAAAACAACAACATTCAAAAACGATTCCCCTCAAAATCTTAGTGGACAAACAAAGCAACAAAGTGGTGTTCGCAGAAGCAAACAAGGATTTCGTTGACACTCTCTTCAGCTTCCTATCTCTTCCACTTGCAACCATTGCTCGCCTTTTAGCCACCATCAACAACAGCAATGATCAACAAAAACAACTCTCAGAATCATCACCATTCCTTGAAAACATCAAAAGCCTCTACCAAACTGTCGAAAACCTTAACTCAGACGATGATGTTTGGAACACCCCTTTCTGCAAACAAATGTTGCTGCATCCCAAGAATCCATGTGAATCACTGTGCATAAAGCTATTCTTGAATATTGACGACACTGAACCATCAAATAAGTTTTTTGTTTGTGATTCTTGTAACAAGTTCACTACTTTTCAAAACCTTCATTGCGCTTGTGGGAAACCAACAAACGGAGAGGCCCAAAATTTGGATTATGAGGGTCACAAACCAGATGCATCAATGTTTCTGGTATCTGATGACTTGAAGATTGTGCGATTTTCCTCTGTGACTTACTTTAAGCTGTTGATGGAGTTGGGGTATTCAGACTTGACTCAACTAGAGGAAGTCACACACAACATTGGCAAGCAGGAGGTAATGATATTGTGTGGTTTTTTGTTTGATATGTATCTATCTCACAATTCCCATACCCTCTCATTACATTGTTCTTTATTTACTTGCATAGGATCTTAGACCAATCTTAATCATTAGCCTCCACCTCGTGGTTTTAATTTTTGGTTTTTCTGCCTACCTTTATTGTGGTAAAATATAGATAAATGCCGCCACAACTTCGGTTATGATGTAGTTGTGGCGACCTCAAAATTTATTATATTGTGGCTGCAGGTGTGGCACGTTTTACCCTTTAGGAAACATAGTACTACTAGGATTTGACGTTGCGTATCAGTTGGTGTTAGGAACCCCAAAATTCAATAGATCTTAAATTTCGAATTTGAAACACAAGGAGTTTGACAAATTTAATATTCACAATCTTAGGATTTAATATTTGACAAGCAAAACAGAATATTAATAAAAAAAGAGTACGAGTACTTCAACCTAAAACAAGAAGACATACTAAGAGAAGAAAAGTACAGTTAGAGAAGTTATTAGTTAGAGGAGGATAATATCCTTTTTTTGTAACAGAATAATAATTTCCCTTAAGACATGTTAGAAGAAATAGCACACATGTTGTACATTTTACGAGCTTTCACATGAATTGAAAGAACTTGAAATCTTTGGAGGATTTATCACTTCTATTCTCATTATTTCATGCAGTAAAATTTCTCTTCTTCCATCTATATTTCTTTCAATCAATTTTTCAATCCTTGGCTAACTCTTAATGTTGTGATTTATTACTGCCAAAAGCAATTGATAATTTCCTTTTTGATGTATAGATATTGAACCTGCTCAAGTATTCCTTAACCTCTAATGAGCCTCTCACAAATACAATTTTGGCATGTAGCTCCATAAATAAAGACAACCCACCTAAGCAATCTGCACCAACAGTTAGAGTATTGCCTTGCACCAATAACAGAAATAAAATGGATGTTAAGATTGTGCAGAGTAAATCTCTGAAAAAAATGATATTCACAGAAGCCAACGGAGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTAGGTTCCATTGTAAACCTTTTGGGACCTAACTCTCTTGCTAGATGTGTTGGTAACTTGTACAAGAGTGTGGAGAACTTGGACCCCAAGTCTGTGTTACTAAATCCTGGTATAGCACCACAGTTTTGTTGTCCAAACCAACCCCTCAACATTCCTTATGTAAAACACCCTACTTATTATTATGCTAAAAGAATACCAAAGCGAAAGAGAGATCCATTCATTTTTCATATAATAGAAGAATTCACTGAGGAAAGAGTGATTTCCAAGACACGTCGATCAGAATCTTATTCATTCTTGACTGCACTCGACCCCCCCAACAGGTCAGAAGAAGGTGTGGTGGGATTTGTGAAGAGAGAAGGATTATATGGTGTAGGAGATGATCTGAGAGTAAAACCACTTTCAGTTTATTTGCGTCTTTCATATTTGAAAGAGTTAAGCCTTCCTCTTGACGATCTTGAAGTGAAGGAAATAAGCATTGGTGAAGCTGAGGTAAGGTCGTTATTATTTATCTCTGAATTTATAATTATTTTCTATTTTTTAACATCTTCGATTCCTCTTGACTTCTGATGCAGGCTTTGAACTTCCTTCGAGCATTCTTGACATCAAAATTTACCCTGACTAGTGCCAGTGGACCAGGAGACCTTTTGAAAGTGCCTAAACAAGAACAAAATTTTACTAACTAG
Protein sequence

>MS.gene50816.t1

MTSQPKQQHSKTIPLKILVDKQSNKVVFAEANKDFVDTLFSFLSLPLATIARLLATINNSNDQQKQLSESSPFLENIKSLYQTVENLNSDDDVWNTPFCKQMLLHPKNPCESLCIKLFLNIDDTEPSNKFFVCDSCNKFTTFQNLHCACGKPTNGEAQNLDYEGHKPDASMFLVSDDLKIVRFSSVTYFKLLMELGYSDLTQLEEVTHNIGKQEILNLLKYSLTSNEPLTNTILACSSINKDNPPKQSAPTVRVLPCTNNRNKMDVKIVQSKSLKKMIFTEANGDFVDFIFSFLTMPLGSIVNLLGPNSLARCVGNLYKSVENLDPKSVLLNPGIAPQFCCPNQPLNIPYVKHPTYYYAKRIPKRKRDPFIFHIIEEFTEERVISKTRRSESYSFLTALDPPNRSEEGVVGFVKREGLYGVGDDLRVKPLSVYLRLSYLKELSLPLDDLEVKEISIGEAEALNFLRAFLTSKFTLTSASGPGDLLKVPKQEQNFTN