AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene068392

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068392.t1 MTR_4g017830 88.162 321 36 1 19 339 213 531 0.0 566
MS.gene068392.t1 MTR_4g017470 63.973 297 102 3 26 318 184 479 3.96e-122 360
MS.gene068392.t1 MTR_4g017470 25.410 244 108 5 78 303 15 202 1.01e-12 69.3
MS.gene068392.t1 MTR_4g017440 64.605 291 96 4 26 315 224 508 2.90e-121 358
MS.gene068392.t1 MTR_4g019340 67.153 274 66 3 26 299 254 503 1.13e-119 354
MS.gene068392.t1 MTR_4g017550 62.718 287 94 7 27 309 196 473 5.18e-109 326
MS.gene068392.t1 MTR_4g017410 58.108 296 92 6 26 316 223 491 6.12e-98 299
MS.gene068392.t1 MTR_4g017410 27.126 247 142 8 78 303 12 241 2.99e-14 73.9
MS.gene068392.t1 MTR_4g017610 50.714 280 113 6 26 305 221 475 2.45e-76 242
MS.gene068392.t1 MTR_4g019310 37.829 304 148 12 26 315 221 497 3.33e-49 172
MS.gene068392.t1 MTR_4g019310 27.459 244 148 7 75 302 8 238 2.77e-13 70.9
MS.gene068392.t1 MTR_7g066400 34.859 284 139 6 28 305 190 433 3.25e-38 141
MS.gene068392.t1 MTR_7g066570 34.471 293 156 8 26 305 207 476 1.47e-36 139
MS.gene068392.t1 MTR_7g066340 31.023 303 149 5 26 320 214 464 3.07e-35 134
MS.gene068392.t1 MTR_7g066340 29.487 234 137 8 75 298 12 227 2.63e-15 77.0
MS.gene068392.t1 MTR_7g066260 32.143 308 155 8 26 325 217 478 4.82e-35 134
MS.gene068392.t1 MTR_7g066260 29.046 241 131 9 78 302 18 234 6.98e-14 72.8
MS.gene068392.t1 MTR_7g066430 35.216 301 172 7 18 311 203 487 1.55e-34 132
MS.gene068392.t1 MTR_7g066410 33.856 319 175 8 8 309 213 512 7.40e-34 130
MS.gene068392.t1 MTR_7g066350 31.818 286 140 7 26 305 209 445 2.47e-33 128
MS.gene068392.t1 MTR_7g066310 34.413 247 116 6 75 320 193 394 2.45e-32 125
MS.gene068392.t1 MTR_7g066310 32.710 107 59 3 75 171 7 110 8.44e-11 63.2
MS.gene068392.t1 MTR_7g058710 29.010 293 192 3 26 315 204 483 9.15e-32 124
MS.gene068392.t1 MTR_7g066280 32.950 261 145 5 78 320 153 401 4.47e-30 119
MS.gene068392.t1 MTR_1g041315 30.403 273 135 6 28 293 227 451 3.95e-29 117
MS.gene068392.t1 MTR_7g062280 30.612 294 176 7 26 315 222 491 5.56e-29 117
MS.gene068392.t1 MTR_7g062280 29.719 249 133 11 76 302 11 239 1.22e-14 75.1
MS.gene068392.t1 MTR_7g066680 30.341 323 168 7 21 305 31 334 1.20e-28 114
MS.gene068392.t1 MTR_7g012680 33.779 299 149 10 26 315 193 451 1.28e-28 115
MS.gene068392.t1 MTR_7g066580 30.068 296 164 9 28 305 167 437 6.46e-27 110
MS.gene068392.t1 MTR_7g063720 32.000 250 132 7 74 320 22 236 1.63e-26 105
MS.gene068392.t1 MTR_7g100420 33.684 285 158 9 27 305 134 393 1.74e-26 108
MS.gene068392.t1 MTR_7g062300 30.175 285 167 8 26 305 212 469 5.94e-26 108
MS.gene068392.t1 MTR_7g062300 30.041 243 130 10 75 302 12 229 3.73e-16 79.3
MS.gene068392.t1 MTR_7g066360 32.168 286 135 9 41 320 228 460 1.11e-25 107
MS.gene068392.t1 MTR_4g019240 76.471 68 16 0 268 335 113 180 2.36e-25 100
MS.gene068392.t1 MTR_7g063900 31.818 286 172 9 27 305 209 478 8.97e-25 104
MS.gene068392.t1 MTR_7g063900 28.112 249 143 8 75 313 10 232 6.36e-17 82.0
MS.gene068392.t1 MTR_7g063920 27.986 293 179 6 26 315 173 436 1.23e-24 103
MS.gene068392.t1 MTR_7g063840 28.231 294 181 8 26 315 206 473 1.84e-24 103
MS.gene068392.t1 MTR_7g063840 33.945 109 65 2 76 177 11 119 5.54e-11 63.9
MS.gene068392.t1 MTR_8g445860 31.818 242 138 7 71 303 9 232 1.34e-23 97.8
MS.gene068392.t1 MTR_7g063660 28.082 292 187 7 27 315 206 477 6.55e-23 99.4
MS.gene068392.t1 MTR_7g063660 28.727 275 141 12 75 328 9 249 2.84e-15 77.0
MS.gene068392.t1 MTR_7g063670 30.241 291 161 9 27 315 207 457 3.28e-22 97.4
MS.gene068392.t1 MTR_0150s0010 31.356 236 142 8 75 303 10 232 1.01e-21 92.8
MS.gene068392.t1 MTR_7g063820 28.912 294 184 6 27 315 211 484 6.67e-21 93.6
MS.gene068392.t1 MTR_7g063820 35.238 105 62 2 73 171 8 112 1.17e-12 68.9
MS.gene068392.t1 MTR_7g063880 29.932 294 167 12 27 315 209 468 2.72e-20 91.7
MS.gene068392.t1 MTR_7g063880 31.381 239 129 10 75 302 11 225 5.18e-15 76.3
MS.gene068392.t1 MTR_3g007740 28.966 290 159 9 26 309 194 442 7.02e-20 90.5
MS.gene068392.t1 MTR_4g019350 44.554 101 44 2 133 233 1 89 2.09e-19 82.0
MS.gene068392.t1 MTR_1g115440 30.126 239 141 6 75 303 11 233 2.68e-19 85.9
MS.gene068392.t1 MTR_7g063830 28.475 295 150 9 26 316 208 445 4.27e-19 88.2
MS.gene068392.t1 MTR_7g063830 37.624 101 56 3 78 171 13 113 1.98e-11 65.1
MS.gene068392.t1 MTR_3g065200 28.085 235 136 7 74 304 211 416 6.06e-19 87.8
MS.gene068392.t1 MTR_7g063850 28.125 256 142 8 72 315 63 288 3.82e-18 84.0
MS.gene068392.t1 MTR_1g014060 33.945 218 100 8 105 315 210 390 6.40e-18 84.3
MS.gene068392.t1 MTR_7g058700 33.108 148 73 5 28 172 725 849 2.04e-16 80.9
MS.gene068392.t1 MTR_7g066290 37.121 132 75 3 41 166 232 361 2.76e-16 79.7
MS.gene068392.t1 MTR_7g063890 30.303 297 184 7 27 316 225 505 6.90e-16 79.0
MS.gene068392.t1 MTR_7g063890 29.362 235 133 9 78 302 30 241 1.19e-13 72.0
MS.gene068392.t1 MTR_7g062270 29.353 201 111 6 71 264 6 182 1.31e-15 77.4
MS.gene068392.t1 MTR_1g115450 43.373 83 45 1 71 151 3 85 5.34e-15 71.6
MS.gene068392.t1 MTR_1g115455 39.759 83 48 1 71 151 5 87 1.50e-13 69.7
MS.gene068392.t1 MTR_8g445920 46.774 62 33 0 75 136 10 71 4.81e-13 65.1
MS.gene068392.t1 MTR_8g044890 46.774 62 33 0 75 136 10 71 4.81e-13 65.1
MS.gene068392.t1 MTR_0150s0030 38.095 84 49 1 71 151 4 87 9.69e-13 67.0
MS.gene068392.t1 MTR_8g445910 39.286 84 48 1 71 151 4 87 2.21e-12 66.6
MS.gene068392.t1 MTR_8g044880 39.286 84 48 1 71 151 4 87 2.92e-12 66.6
MS.gene068392.t1 MTR_1g115470 41.463 82 46 1 72 151 3 84 7.60e-12 66.2
MS.gene068392.t1 MTR_7g066490 28.966 290 125 9 24 309 195 407 1.36e-11 65.5
MS.gene068392.t1 MTR_7g066480 37.589 141 77 5 24 157 209 345 3.33e-11 64.3
MS.gene068392.t1 MTR_4g019330 43.529 85 46 2 233 315 104 188 4.98e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene068392.t1 AT5G01120 30.000 290 174 8 26 305 215 485 1.78e-26 110
MS.gene068392.t1 AT5G01140 28.859 298 181 9 26 319 191 461 7.40e-24 102
MS.gene068392.t1 AT5G01130 29.110 292 160 10 27 315 204 451 7.88e-24 102
MS.gene068392.t1 AT3G09140 28.523 298 161 10 26 305 254 517 1.47e-20 92.8
MS.gene068392.t1 AT3G09140 26.829 205 134 6 75 268 9 208 4.33e-11 64.3
MS.gene068392.t1 AT3G09140 28.620 297 160 10 27 305 227 489 2.03e-20 92.4
MS.gene068392.t1 AT3G09140 27.083 240 158 7 75 302 9 243 2.27e-13 71.2
MS.gene068392.t1 AT5G37320 27.055 292 166 9 26 307 223 477 3.18e-19 89.0
MS.gene068392.t1 AT5G43240 29.720 286 171 9 31 305 79 345 1.07e-17 83.6
MS.gene068392.t1 AT5G43240 29.720 286 171 9 31 305 223 489 2.91e-17 83.2
MS.gene068392.t1 AT5G43240 26.966 267 148 10 78 324 12 251 2.92e-13 70.9
MS.gene068392.t1 AT5G43240 29.720 286 171 9 31 305 223 489 2.91e-17 83.2
MS.gene068392.t1 AT5G43240 26.966 267 148 10 78 324 12 251 2.92e-13 70.9
MS.gene068392.t1 AT5G43240 29.720 286 171 9 31 305 223 489 2.91e-17 83.2
MS.gene068392.t1 AT5G43240 26.966 267 148 10 78 324 12 251 2.92e-13 70.9
MS.gene068392.t1 AT5G01150 28.814 236 149 6 84 318 272 489 1.40e-16 80.9
MS.gene068392.t1 AT3G09120 27.119 236 153 5 75 303 6 229 3.01e-13 70.1
MS.gene068392.t1 AT3G09110 25.311 241 143 6 75 302 9 225 3.53e-11 63.9

Find 67 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCACCCACTACTTGTTATTA+TGG 0.206586 4.2:-83850549 MS.gene068392:CDS
AGGAGTGATTTCTAAGAAAA+AGG 0.237632 4.2:-83850454 MS.gene068392:CDS
GTCAAATTTGATTCTTGTTT+TGG 0.240264 4.2:+83849774 None:intergenic
TTGCACCAGCGACGTTAAAA+TGG 0.290575 4.2:-83850859 MS.gene068392:CDS
GGTGGTTGTGGTTGTGCATT+AGG 0.307334 4.2:+83850567 None:intergenic
TTTGACTTGATTGAGGATCT+TGG 0.316826 4.2:+83850393 None:intergenic
AGGGTCCGTTGTAAAGCTTT+TGG 0.360145 4.2:-83850736 MS.gene068392:CDS
ACTGCTGATGCAAATTCCTT+TGG 0.374617 4.2:+83850894 None:intergenic
AAAGCAACATTATATGCTAT+TGG 0.383153 4.2:-83850345 MS.gene068392:CDS
CAGAGGCTCACGAGAGGTTA+AGG 0.404803 4.2:+83850962 None:intergenic
TAGTGTTCAGCGACTATAAT+GGG 0.412179 4.2:-83850509 MS.gene068392:CDS
ATAATTCAGGCATCTGCTAT+AGG 0.419735 4.2:+83849705 None:intergenic
CTAGTGTTCAGCGACTATAA+TGG 0.430095 4.2:-83850510 MS.gene068392:CDS
CCTGGCCTATCACCATGGTT+TGG 0.435447 4.2:-83850615 MS.gene068392:CDS
ACTACTTGTTATTATGGTAC+AGG 0.436855 4.2:-83850543 MS.gene068392:CDS
GGAATGTTGAGGAGTTGGTT+TGG 0.438474 4.2:+83850588 None:intergenic
GCTGATGCAAATTCCTTTGG+TGG 0.439130 4.2:+83850897 None:intergenic
GATTCGACTTTACTCTGGTT+CGG 0.439315 4.2:+83851776 None:intergenic
TCCAATGCAGTCAAGGCTCT+TGG 0.465938 4.2:+83850417 None:intergenic
GACAACCAAACCATGGTGAT+AGG 0.468422 4.2:+83850610 None:intergenic
ATGCAAGAATTATCAGAAAG+TGG 0.472857 4.2:+83850303 None:intergenic
CCTTCATTTCCAGATCATCA+AGG 0.480594 4.2:+83850253 None:intergenic
GTACAAGACTATTGAGACCT+TGG 0.488174 4.2:-83850679 MS.gene068392:CDS
CATTAGGAATGTTGAGGAGT+TGG 0.490761 4.2:+83850583 None:intergenic
AAGTCAAAAGAAGGTGTTGT+GGG 0.497331 4.2:-83850378 MS.gene068392:CDS
TCGAGGTGCTGTGGCACGTA+AGG 0.501548 4.2:-83851749 MS.gene068392:intron
AGCTTTACAACGGACCCTAA+TGG 0.502068 4.2:+83850741 None:intergenic
TTGCATCAGCAGTCAGAGTA+AGG 0.502602 4.2:-83850884 MS.gene068392:CDS
CGCTCCGTGTTACTCAATCC+TGG 0.507746 4.2:-83850633 MS.gene068392:CDS
TAGTCGCTGAACACTAGCTT+TGG 0.516019 4.2:+83850516 None:intergenic
ACCAAGAGCCTTGACTGCAT+TGG 0.516962 4.2:-83850418 MS.gene068392:CDS
TCCTCAATCAAGTCAAAAGA+AGG 0.520771 4.2:-83850387 MS.gene068392:CDS
TTCAAAATTGTATTTGTCAG+AGG 0.520812 4.2:+83850945 None:intergenic
TAACAAGTAGTGGGTGGTTG+TGG 0.521856 4.2:+83850555 None:intergenic
GTAGAAGAAAAGATAAAGAG+AGG 0.525245 4.2:-83850474 MS.gene068392:CDS
TCAAAATTTACACTGACTAG+TGG 0.539589 4.2:-83849819 MS.gene068392:CDS
ACTGTGATTCGACTTTACTC+TGG 0.546990 4.2:+83851771 None:intergenic
CTTCATTTCCAGATCATCAA+GGG 0.555681 4.2:+83850254 None:intergenic
CAAAGTATATACAAACTAGC+AGG 0.558931 4.2:-83849749 MS.gene068392:CDS
CCTTGATGATCTGGAAATGA+AGG 0.561579 4.2:-83850253 MS.gene068392:CDS
CTTGGACTCTTCATTGTGCA+CGG 0.565969 4.2:-83850661 MS.gene068392:CDS
CCAAACCATGGTGATAGGCC+AGG 0.566603 4.2:+83850615 None:intergenic
TCTTGGATCCAATGCAGTCA+AGG 0.569511 4.2:+83850410 None:intergenic
CTACTTGTTATTATGGTACA+GGG 0.573529 4.2:-83850542 MS.gene068392:CDS
CAGGCTTGATGAACTTGCAA+AGG 0.580987 4.2:-83849730 MS.gene068392:CDS
TGTACCATAATAACAAGTAG+TGG 0.600592 4.2:+83850545 None:intergenic
TTGATGTAATGGAAGCTGCA+AGG 0.606907 4.2:+83849838 None:intergenic
GAAATGAAGGTGAAAAGCAT+CGG 0.612181 4.2:-83850240 MS.gene068392:CDS
CGTGCACAATGAAGAGTCCA+AGG 0.613164 4.2:+83850662 None:intergenic
GAACAAAGAAAATCCACCAA+AGG 0.613320 4.2:-83850910 MS.gene068392:CDS
CAAGTCAAAAGAAGGTGTTG+TGG 0.614507 4.2:-83850379 MS.gene068392:CDS
AGGTTAAGGTGTACTTGAGG+AGG 0.618815 4.2:+83850976 None:intergenic
GAATCACAGTCGAGGTGCTG+TGG 0.619480 4.2:-83851758 MS.gene068392:CDS
TCAATCCTGGCCTATCACCA+TGG 0.620012 4.2:-83850620 MS.gene068392:CDS
GTACCATAATAACAAGTAGT+GGG 0.629726 4.2:+83850546 None:intergenic
GAAAAGCATCGGTGAAGCTG+AGG 0.637156 4.2:-83850229 MS.gene068392:intron
TTCATTTCCAGATCATCAAG+GGG 0.637868 4.2:+83850255 None:intergenic
ACCATCCAAAAGCTTTACAA+CGG 0.652754 4.2:+83850731 None:intergenic
TGGTTTGGACAACCAAACCA+TGG 0.676530 4.2:+83850603 None:intergenic
CCATAATAACAAGTAGTGGG+TGG 0.678221 4.2:+83850549 None:intergenic
GAGAGGTTAAGGTGTACTTG+AGG 0.692261 4.2:+83850973 None:intergenic
GTAAAGTCGAATCACAGTCG+AGG 0.700846 4.2:-83851766 MS.gene068392:CDS
ATTTGTCAGAGGCTCACGAG+AGG 0.701484 4.2:+83850956 None:intergenic
GTTGTGCATTAGGAATGTTG+AGG 0.729602 4.2:+83850577 None:intergenic
ATGTAATGGAAGCTGCAAGG+AGG 0.730424 4.2:+83849841 None:intergenic
TCATTTCCAGATCATCAAGG+GGG 0.747642 4.2:+83850256 None:intergenic
TAGGCCAGGATTGAGTAACA+CGG 0.748218 4.2:+83850629 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATAAACATTCACAATTT+GGG - chr4.2:83850340-83850359 MS.gene068392:CDS 15.0%
!! TTTAATAAACATTCACAATT+TGG - chr4.2:83850339-83850358 MS.gene068392:CDS 15.0%
!!! TTGTAATTTTAATACATTCA+AGG - chr4.2:83851541-83851560 MS.gene068392:intron 15.0%
!! AAAAAAAAGTGATAAACTCT+AGG + chr4.2:83850150-83850169 None:intergenic 20.0%
!!! AAATAACTTGTAACATTCAA+AGG + chr4.2:83850453-83850472 None:intergenic 20.0%
!!! TTGAATGTTACAAGTTATTT+TGG - chr4.2:83850453-83850472 MS.gene068392:CDS 20.0%
!!! TTGTTGCTATATTTAAGTTA+TGG - chr4.2:83850385-83850404 MS.gene068392:CDS 20.0%
! AAAGCAACATTATATGCTAT+TGG - chr4.2:83851173-83851192 MS.gene068392:intron 25.0%
! AAATTGTAAACCACAAAAGA+AGG + chr4.2:83851645-83851664 None:intergenic 25.0%
! CCAATATAACAGATGAATTA+TGG - chr4.2:83849920-83849939 MS.gene068392:intron 25.0%
! CCATAATTCATCTGTTATAT+TGG + chr4.2:83849923-83849942 None:intergenic 25.0%
! TGGTGTACCATAAATTAAAA+GGG - chr4.2:83850307-83850326 MS.gene068392:CDS 25.0%
! TTCAAAATTGTATTTGTCAG+AGG + chr4.2:83850576-83850595 None:intergenic 25.0%
! TTGGTGTACCATAAATTAAA+AGG - chr4.2:83850306-83850325 MS.gene068392:CDS 25.0%
!! ACTTTTCTTACAATACCATT+AGG - chr4.2:83850762-83850781 MS.gene068392:CDS 25.0%
!! CTTTTCTTACAATACCATTA+GGG - chr4.2:83850763-83850782 MS.gene068392:CDS 25.0%
!!! ATGTTCACCCTTTTAATTTA+TGG + chr4.2:83850317-83850336 None:intergenic 25.0%
!!! CAGTGTAAATTTTGATGTAA+TGG + chr4.2:83851694-83851713 None:intergenic 25.0%
!!! GTCAAATTTGATTCTTGTTT+TGG + chr4.2:83851747-83851766 None:intergenic 25.0%
AATAGACAGGAAAGTAAAGA+GGG - chr4.2:83850014-83850033 MS.gene068392:intron 30.0%
ACAAAGTAACAGAATGTAGT+TGG - chr4.2:83850058-83850077 MS.gene068392:intron 30.0%
ATGCAAGAATTATCAGAAAG+TGG + chr4.2:83851218-83851237 None:intergenic 30.0%
CAAAGTATATACAAACTAGC+AGG - chr4.2:83851769-83851788 MS.gene068392:CDS 30.0%
GAATAAGCTAAGGTAAAAGT+GGG + chr4.2:83850260-83850279 None:intergenic 30.0%
GTACCATAATAACAAGTAGT+GGG + chr4.2:83850975-83850994 None:intergenic 30.0%
GTAGAAGAAAAGATAAAGAG+AGG - chr4.2:83851044-83851063 MS.gene068392:intron 30.0%
TAATCAATGAAAGACGCATA+TGG - chr4.2:83851470-83851489 MS.gene068392:intron 30.0%
TCATAATTAACTAACACCTG+TGG + chr4.2:83849866-83849885 None:intergenic 30.0%
TGAATAAGCTAAGGTAAAAG+TGG + chr4.2:83850261-83850280 None:intergenic 30.0%
TGTACCATAATAACAAGTAG+TGG + chr4.2:83850976-83850995 None:intergenic 30.0%
! AGGAGTGATTTCTAAGAAAA+AGG - chr4.2:83851064-83851083 MS.gene068392:intron 30.0%
! AGTAAGAACTAAGAGAGTTT+TGG - chr4.2:83850108-83850127 MS.gene068392:intron 30.0%
! CTTTTGTTAAGTCATAAGTC+AGG + chr4.2:83851503-83851522 None:intergenic 30.0%
! GATGAATTATGGTTTGCATT+TGG - chr4.2:83849931-83849950 MS.gene068392:intron 30.0%
! GTAAGAACTAAGAGAGTTTT+GGG - chr4.2:83850109-83850128 MS.gene068392:intron 30.0%
! GTATCAAAATGCTGCTTAAA+AGG - chr4.2:83849886-83849905 MS.gene068392:intron 30.0%
! TAAGAACTAAGAGAGTTTTG+GGG - chr4.2:83850110-83850129 MS.gene068392:intron 30.0%
! TCAAAATTTACACTGACTAG+TGG - chr4.2:83851699-83851718 MS.gene068392:intron 30.0%
! TTTTGTGGTTTACAATTTGC+AGG - chr4.2:83851647-83851666 MS.gene068392:intron 30.0%
!! ACTACTTGTTATTATGGTAC+AGG - chr4.2:83850975-83850994 MS.gene068392:CDS 30.0%
!! CTACTTGTTATTATGGTACA+GGG - chr4.2:83850976-83850995 MS.gene068392:CDS 30.0%
!!! TTGTTGATAACCTTCTTTTG+TGG - chr4.2:83851632-83851651 MS.gene068392:intron 30.0%
AAGTAAAGAGGGAAATAGAG+TGG - chr4.2:83850025-83850044 MS.gene068392:intron 35.0%
AAGTAACAGAATGTAGTTGG+AGG - chr4.2:83850061-83850080 MS.gene068392:intron 35.0%
AATGTAGTTGGAGGGAATAA+GGG - chr4.2:83850070-83850089 MS.gene068392:intron 35.0%
ACCATCCAAAAGCTTTACAA+CGG + chr4.2:83850790-83850809 None:intergenic 35.0%
AGTAACAGAATGTAGTTGGA+GGG - chr4.2:83850062-83850081 MS.gene068392:intron 35.0%
ATTCATGCTTCCACATAAAG+TGG - chr4.2:83850276-83850295 MS.gene068392:CDS 35.0%
ATTGGTTAACATGTTAGTCC+AGG - chr4.2:83849830-83849849 MS.gene068392:CDS 35.0%
CTTCATTTCCAGATCATCAA+GGG + chr4.2:83851267-83851286 None:intergenic 35.0%
GAACAAAGAAAATCCACCAA+AGG - chr4.2:83850608-83850627 MS.gene068392:CDS 35.0%
GAATAGACAGGAAAGTAAAG+AGG - chr4.2:83850013-83850032 MS.gene068392:intron 35.0%
GTTTGCATTTGGCATAGTAT+CGG - chr4.2:83849942-83849961 MS.gene068392:intron 35.0%
TAGTGTTCAGCGACTATAAT+GGG - chr4.2:83851009-83851028 MS.gene068392:intron 35.0%
TCCTCAATCAAGTCAAAAGA+AGG - chr4.2:83851131-83851150 MS.gene068392:intron 35.0%
TGATCCTTAAATGTGTGAGT+CGG + chr4.2:83851577-83851596 None:intergenic 35.0%
TGTTAAGTCATAAGTCAGGT+TGG + chr4.2:83851499-83851518 None:intergenic 35.0%
TTCATTTCCAGATCATCAAG+GGG + chr4.2:83851266-83851285 None:intergenic 35.0%
TTGGTTAACATGTTAGTCCA+GGG - chr4.2:83849831-83849850 MS.gene068392:CDS 35.0%
! GAAATGAAGGTGAAAAGCAT+CGG - chr4.2:83851278-83851297 MS.gene068392:intron 35.0%
! GGCTAGTTGTTAAAGTTATG+AGG - chr4.2:83850361-83850380 MS.gene068392:CDS 35.0%
! TTGGATGGTAACTCTTTTGT+TGG - chr4.2:83850801-83850820 MS.gene068392:CDS 35.0%
! TTTTATCATGAACAACCCTC+TGG + chr4.2:83850190-83850209 None:intergenic 35.0%
!! AAGTCAAAAGAAGGTGTTGT+GGG - chr4.2:83851140-83851159 MS.gene068392:intron 35.0%
!! GTATCGGTTAAGAACTTGAT+AGG - chr4.2:83849958-83849977 MS.gene068392:intron 35.0%
!! TTTGACTTGATTGAGGATCT+TGG + chr4.2:83851128-83851147 None:intergenic 35.0%
!!! ACCTTCTTTTGACTTGATTG+AGG + chr4.2:83851135-83851154 None:intergenic 35.0%
ACATGGACCTTCTTAATGAG+TGG + chr4.2:83849987-83850006 None:intergenic 40.0%
ACTGCTGATGCAAATTCCTT+TGG + chr4.2:83850627-83850646 None:intergenic 40.0%
ACTGTGATTCGACTTTACTC+TGG + chr4.2:83849750-83849769 None:intergenic 40.0%
ATGTAGTTGGAGGGAATAAG+GGG - chr4.2:83850071-83850090 MS.gene068392:intron 40.0%
CATTAGGAATGTTGAGGAGT+TGG + chr4.2:83850938-83850957 None:intergenic 40.0%
CCTTCATTTCCAGATCATCA+AGG + chr4.2:83851268-83851287 None:intergenic 40.0%
CTAGTGTTCAGCGACTATAA+TGG - chr4.2:83851008-83851027 MS.gene068392:intron 40.0%
CTGTTGCAATTCTCCTTCAT+GGG - chr4.2:83850232-83850251 MS.gene068392:CDS 40.0%
GAATGTAGTTGGAGGGAATA+AGG - chr4.2:83850069-83850088 MS.gene068392:intron 40.0%
GAATTGCAACAGGAATGTGA+TGG + chr4.2:83850224-83850243 None:intergenic 40.0%
GATTCGACTTTACTCTGGTT+CGG + chr4.2:83849745-83849764 None:intergenic 40.0%
GCTGCTTAAAAGGTAATGTG+TGG - chr4.2:83849896-83849915 MS.gene068392:intron 40.0%
GTACAAGACTATTGAGACCT+TGG - chr4.2:83850839-83850858 MS.gene068392:CDS 40.0%
GTTGTGCATTAGGAATGTTG+AGG + chr4.2:83850944-83850963 None:intergenic 40.0%
TAGGAGACCACTCATTAAGA+AGG - chr4.2:83849977-83849996 MS.gene068392:intron 40.0%
TCATTTCCAGATCATCAAGG+GGG + chr4.2:83851265-83851284 None:intergenic 40.0%
! CCATAATAACAAGTAGTGGG+TGG + chr4.2:83850972-83850991 None:intergenic 40.0%
! CCTTGATGATCTGGAAATGA+AGG - chr4.2:83851265-83851284 MS.gene068392:intron 40.0%
! GTGGAAGCATGAATAAGCTA+AGG + chr4.2:83850270-83850289 None:intergenic 40.0%
! TCCGTTGTAAAGCTTTTGGA+TGG - chr4.2:83850786-83850805 MS.gene068392:CDS 40.0%
! TTGATGTAATGGAAGCTGCA+AGG + chr4.2:83851683-83851702 None:intergenic 40.0%
!! AACATCCATTTTAACGTCGC+TGG + chr4.2:83850667-83850686 None:intergenic 40.0%
!! CAAGTCAAAAGAAGGTGTTG+TGG - chr4.2:83851139-83851158 MS.gene068392:intron 40.0%
!! CCACCCACTACTTGTTATTA+TGG - chr4.2:83850969-83850988 MS.gene068392:CDS 40.0%
AGCTTTACAACGGACCCTAA+TGG + chr4.2:83850780-83850799 None:intergenic 45.0%
AGGTTAAGGTGTACTTGAGG+AGG + chr4.2:83850545-83850564 None:intergenic 45.0%
CAGGCTTGATGAACTTGCAA+AGG - chr4.2:83851788-83851807 MS.gene068392:CDS 45.0%
CCATGAAGGAGAATTGCAAC+AGG + chr4.2:83850234-83850253 None:intergenic 45.0%
CCATGTGAGACAGAATAGAC+AGG - chr4.2:83850001-83850020 MS.gene068392:intron 45.0%
CCTGTCTATTCTGTCTCACA+TGG + chr4.2:83850004-83850023 None:intergenic 45.0%
CCTGTTGCAATTCTCCTTCA+TGG - chr4.2:83850231-83850250 MS.gene068392:CDS 45.0%
CTGAGGTAAGCGACTACTAT+TGG - chr4.2:83851306-83851325 MS.gene068392:intron 45.0%
CTTGGACTCTTCATTGTGCA+CGG - chr4.2:83850857-83850876 MS.gene068392:CDS 45.0%
GACAACCAAACCATGGTGAT+AGG + chr4.2:83850911-83850930 None:intergenic 45.0%
GAGAGGTTAAGGTGTACTTG+AGG + chr4.2:83850548-83850567 None:intergenic 45.0%
GCTGATGCAAATTCCTTTGG+TGG + chr4.2:83850624-83850643 None:intergenic 45.0%
GGACTCAATCTTGGCTACAT+TGG - chr4.2:83849812-83849831 MS.gene068392:CDS 45.0%
GTAAAGTCGAATCACAGTCG+AGG - chr4.2:83849752-83849771 MS.gene068392:CDS 45.0%
GTGTCTTCTGCTAGTCAGTT+TGG + chr4.2:83851435-83851454 None:intergenic 45.0%
TAGGCCAGGATTGAGTAACA+CGG + chr4.2:83850892-83850911 None:intergenic 45.0%
TCTGTTCCTATAGCTCCAGA+GGG - chr4.2:83850172-83850191 MS.gene068392:intron 45.0%
TCTTGGATCCAATGCAGTCA+AGG + chr4.2:83851111-83851130 None:intergenic 45.0%
TGCTTCCACATAAAGTGGCT+TGG - chr4.2:83850281-83850300 MS.gene068392:CDS 45.0%
TTGCACCAGCGACGTTAAAA+TGG - chr4.2:83850659-83850678 MS.gene068392:CDS 45.0%
TTGCATCAGCAGTCAGAGTA+AGG - chr4.2:83850634-83850653 MS.gene068392:CDS 45.0%
! AGGGTCCGTTGTAAAGCTTT+TGG - chr4.2:83850782-83850801 MS.gene068392:CDS 45.0%
! ATGTAATGGAAGCTGCAAGG+AGG + chr4.2:83851680-83851699 None:intergenic 45.0%
! CAAGACCAAGCCACTTTATG+TGG + chr4.2:83850289-83850308 None:intergenic 45.0%
! GGAATGTTGAGGAGTTGGTT+TGG + chr4.2:83850933-83850952 None:intergenic 45.0%
! TAGTCGCTGAACACTAGCTT+TGG + chr4.2:83851005-83851024 None:intergenic 45.0%
! TGGTTTGGACAACCAAACCA+TGG + chr4.2:83850918-83850937 None:intergenic 45.0%
!! ATTTTAACGTCGCTGGTGCA+AGG + chr4.2:83850660-83850679 None:intergenic 45.0%
!! CACATAAAGTGGCTTGGTCT+TGG - chr4.2:83850287-83850306 MS.gene068392:CDS 45.0%
!! GACACCGACTCACACATTTA+AGG - chr4.2:83851570-83851589 MS.gene068392:intron 45.0%
!! TAACAAGTAGTGGGTGGTTG+TGG + chr4.2:83850966-83850985 None:intergenic 45.0%
AAGATTGAGTCCCAGCTCGT+AGG + chr4.2:83849804-83849823 None:intergenic 50.0%
ACCAAGAGCCTTGACTGCAT+TGG - chr4.2:83851100-83851119 MS.gene068392:intron 50.0%
ACCTGTGGTCTAACTAACCC+TGG + chr4.2:83849851-83849870 None:intergenic 50.0%
ATTTGTCAGAGGCTCACGAG+AGG + chr4.2:83850565-83850584 None:intergenic 50.0%
CGTGCACAATGAAGAGTCCA+AGG + chr4.2:83850859-83850878 None:intergenic 50.0%
CTCTGTTCCTATAGCTCCAG+AGG - chr4.2:83850171-83850190 MS.gene068392:intron 50.0%
GGTGGTTGTGGTTGTGCATT+AGG + chr4.2:83850954-83850973 None:intergenic 50.0%
GTAAAAGTGGGAGCCCATGA+AGG + chr4.2:83850248-83850267 None:intergenic 50.0%
TACGAGCTGGGACTCAATCT+TGG - chr4.2:83849803-83849822 MS.gene068392:CDS 50.0%
TCAATCCTGGCCTATCACCA+TGG - chr4.2:83850898-83850917 MS.gene068392:CDS 50.0%
TCCAATGCAGTCAAGGCTCT+TGG + chr4.2:83851104-83851123 None:intergenic 50.0%
TTTCCAGATCATCAAGGGGG+AGG + chr4.2:83851262-83851281 None:intergenic 50.0%
!! GAAAAGCATCGGTGAAGCTG+AGG - chr4.2:83851289-83851308 MS.gene068392:intron 50.0%
!! GAACAACCCTCTGGAGCTAT+AGG + chr4.2:83850181-83850200 None:intergenic 50.0%
!! TCCAGGGTTAGTTAGACCAC+AGG - chr4.2:83849847-83849866 MS.gene068392:CDS 50.0%
CAGAGGCTCACGAGAGGTTA+AGG + chr4.2:83850559-83850578 None:intergenic 55.0%
CCAAACCATGGTGATAGGCC+AGG + chr4.2:83850906-83850925 None:intergenic 55.0%
CCTGGCCTATCACCATGGTT+TGG - chr4.2:83850903-83850922 MS.gene068392:CDS 55.0%
GTCGTGTGATCCTACGAGCT+GGG - chr4.2:83849791-83849810 MS.gene068392:CDS 55.0%
! CGCTCCGTGTTACTCAATCC+TGG - chr4.2:83850885-83850904 MS.gene068392:CDS 55.0%
!! GAATCACAGTCGAGGTGCTG+TGG - chr4.2:83849760-83849779 MS.gene068392:CDS 55.0%
GGTCGTGTGATCCTACGAGC+TGG - chr4.2:83849790-83849809 MS.gene068392:CDS 60.0%
! GAGCCTCCCCCTTGATGATC+TGG - chr4.2:83851256-83851275 MS.gene068392:intron 60.0%
!! TCGAGGTGCTGTGGCACGTA+AGG - chr4.2:83849769-83849788 MS.gene068392:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 83849710 83851830 83849710 ID=MS.gene068392
chr4.2 mRNA 83849710 83851830 83849710 ID=MS.gene068392.t1;Parent=MS.gene068392
chr4.2 exon 83851750 83851830 83851750 ID=MS.gene068392.t1.exon1;Parent=MS.gene068392.t1
chr4.2 CDS 83851750 83851830 83851750 ID=cds.MS.gene068392.t1;Parent=MS.gene068392.t1
chr4.2 exon 83850230 83851006 83850230 ID=MS.gene068392.t1.exon2;Parent=MS.gene068392.t1
chr4.2 CDS 83850230 83851006 83850230 ID=cds.MS.gene068392.t1;Parent=MS.gene068392.t1
chr4.2 exon 83849710 83849871 83849710 ID=MS.gene068392.t1.exon3;Parent=MS.gene068392.t1
chr4.2 CDS 83849710 83849871 83849710 ID=cds.MS.gene068392.t1;Parent=MS.gene068392.t1
Gene Sequence

>MS.gene068392

ATGGCAAAAGAAACAAAGGAAACAGAGCAAAACCGAACCAGAGTAAAGTCGAATCACAGTCGAGGTGCTGTGGCACGTAAGGTCGTGTGATCCTACGAGCTGGGACTCAATCTTGGCTACATTGGTTAACATGTTAGTCCAGGGTTAGTTAGACCACAGGTGTTAGTTAATTATGAGTATCAAAATGCTGCTTAAAAGGTAATGTGTGGACCAATATAACAGATGAATTATGGTTTGCATTTGGCATAGTATCGGTTAAGAACTTGATAGGAGACCACTCATTAAGAAGGTCCATGTGAGACAGAATAGACAGGAAAGTAAAGAGGGAAATAGAGTGGTTATACACTGACAAAGTAACAGAATGTAGTTGGAGGGAATAAGGGGATGTTGCAGAAGTAAGTAAGAACTAAGAGAGTTTTGGGGCACTTTTGAATTAGCCTAGAGTTTATCACTTTTTTTTGCTCTGTTCCTATAGCTCCAGAGGGTTGTTCATGATAAAATTCTTCTTTTTCCATCACATTCCTGTTGCAATTCTCCTTCATGGGCTCCCACTTTTACCTTAGCTTATTCATGCTTCCACATAAAGTGGCTTGGTCTTGGTGTACCATAAATTAAAAGGGTGAACATTATTTAATAAACATTCACAATTTGGGCTAGTTGTTAAAGTTATGAGGCTTGTTGCTATATTTAAGTTATGGAAACACTATAAATCTTGAGTTAGAAATTGTTAGTAATTATGTCCTTTGAATGTTACAAGTTATTTTGGTTGTAATTAAAATATCTTGCATAATATCTTGTACTTACAAGTTATTTTGATGTAACAGATACTGAACCTCCTCAAGTACACCTTAACCTCTCGTGAGCCTCTGACAAATACAATTTTGAAAAGTAGCTCAAAGAACAAAGAAAATCCACCAAAGGAATTTGCATCAGCAGTCAGAGTAAGGCCTTGCACCAGCGACGTTAAAATGGATGTTAAGCTTGCACGAAGTAAATCTCAGAAAAAAATAATCTTCGCAGAAGCGAGTGAAAACTTTGTTGATTTCATTTTCACTTTTCTTACAATACCATTAGGGTCCGTTGTAAAGCTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGACTATTGAGACCTTGGACTCTTCATTGTGCACGGACTCGCGCTCCGTGTTACTCAATCCTGGCCTATCACCATGGTTTGGTTGTCCAAACCAACTCCTCAACATTCCTAATGCACAACCACAACCACCCACTACTTGTTATTATGGTACAGGGACGCCAAAGCTAGTGTTCAGCGACTATAATGGGAGTCATCAAATTGTAGAAGAAAAGATAAAGAGAGGAGTGATTTCTAAGAAAAAGGTATCAATTTATAAACCAAGAGCCTTGACTGCATTGGATCCAAGATCCTCAATCAAGTCAAAAGAAGGTGTTGTGGGATTTGTGAAGAAAGCAACATTATATGCTATTGGAGATGATCTAACAGTAAAACCACTTTCTGATAATTCTTGCATTTCATATTTGAAAGAATTGAGCCTCCCCCTTGATGATCTGGAAATGAAGGTGAAAAGCATCGGTGAAGCTGAGGTAAGCGACTACTATTGGTTCTTAAATGTGTGAGACAGTATATTACTATAGTTCTGCGTGTATCGAAATTACATAAAATACATAAAAACATTCATCAAGTCTTTTATTTGTAATTTATGAACCAAACTGACTAGCAGAAGACACATTTGAGAATCAAACTAATCAATGAAAGACGCATATGGAAACCAACCTGACTTATGACTTAACAAAAGAAACATTCATGTATGTTCTTGTAATTTTAATACATTCAAGGACTAGTGACACCGACTCACACATTTAAGGATCAAAATCGTTATTTACTCTTTTCTTTTGTTTTCTTTGTTGTTGATAACCTTCTTTTGTGGTTTACAATTTGCAGGCGTTGAGCCTCCTTGCAGCTTCCATTACATCAAAATTTACACTGACTAGTGGACTAGAAGACTTTTTTGTTGTGCCAAAACAAGAATCAAATTTGACATCAAAGTATATACAAACTAGCAGGCTTGATGAACTTGCAAAGGAGCCTATAGCAGATGCCTGA
Protein sequence

>MS.gene068392.t1

MAKETKETEQNRTRVKSNHSRGAVARKILNLLKYTLTSREPLTNTILKSSSKNKENPPKEFASAVRVRPCTSDVKMDVKLARSKSQKKIIFAEASENFVDFIFTFLTIPLGSVVKLLDGNSFVGCVDNLYKTIETLDSSLCTDSRSVLLNPGLSPWFGCPNQLLNIPNAQPQPPTTCYYGTGTPKLVFSDYNGSHQIVEEKIKRGVISKKKVSIYKPRALTALDPRSSIKSKEGVVGFVKKATLYAIGDDLTVKPLSDNSCISYLKELSLPLDDLEMKVKSIGEAEALSLLAASITSKFTLTSGLEDFFVVPKQESNLTSKYIQTSRLDELAKEPIADA