AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene055086

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055086.t1 MTR_7g066340 79.710 483 88 3 1 480 1 476 0.0 762
MS.gene055086.t1 MTR_7g066310 68.220 472 81 4 9 480 4 406 0.0 617
MS.gene055086.t1 MTR_7g066310 27.826 230 124 7 8 236 189 377 1.51e-12 69.7
MS.gene055086.t1 MTR_7g066350 63.983 472 145 8 1 461 1 458 0.0 557
MS.gene055086.t1 MTR_1g041315 64.414 444 146 5 8 441 10 451 0.0 555
MS.gene055086.t1 MTR_7g066280 63.636 440 83 3 91 480 1 413 0.0 535
MS.gene055086.t1 MTR_7g066280 26.894 264 133 11 8 236 146 384 1.66e-11 66.2
MS.gene055086.t1 MTR_7g066260 57.576 495 179 11 6 480 9 492 0.0 520
MS.gene055086.t1 MTR_7g066570 55.666 503 170 9 6 472 8 493 4.06e-171 494
MS.gene055086.t1 MTR_7g066400 56.250 464 163 5 1 454 1 434 9.22e-170 486
MS.gene055086.t1 MTR_7g066360 52.953 491 194 9 6 479 8 478 3.58e-154 447
MS.gene055086.t1 MTR_7g012680 53.151 476 179 7 3 463 5 451 2.79e-153 444
MS.gene055086.t1 MTR_7g066290 54.902 459 168 9 6 458 9 434 3.32e-151 438
MS.gene055086.t1 MTR_7g058710 47.904 501 193 11 1 457 1 477 3.45e-138 407
MS.gene055086.t1 MTR_7g066580 47.571 494 164 14 18 463 1 447 3.47e-131 387
MS.gene055086.t1 MTR_7g066580 26.984 252 146 8 6 238 209 441 2.62e-12 68.9
MS.gene055086.t1 MTR_7g066410 40.039 507 239 9 12 457 10 512 3.70e-115 348
MS.gene055086.t1 MTR_7g058700 46.309 447 171 7 24 457 531 921 6.06e-113 355
MS.gene055086.t1 MTR_7g066430 41.498 494 229 12 12 457 4 485 2.13e-110 336
MS.gene055086.t1 MTR_7g066430 26.772 254 132 8 9 236 254 479 1.58e-13 73.2
MS.gene055086.t1 MTR_2g029400 44.211 475 151 10 6 445 8 403 7.89e-108 326
MS.gene055086.t1 MTR_7g066480 40.570 456 217 10 11 459 15 423 6.55e-98 301
MS.gene055086.t1 MTR_7g066490 41.593 452 202 12 12 459 16 409 1.51e-97 300
MS.gene055086.t1 MTR_7g063660 36.570 484 256 9 8 457 5 471 5.68e-94 293
MS.gene055086.t1 MTR_7g062300 37.860 486 252 13 1 453 1 469 2.45e-93 291
MS.gene055086.t1 MTR_7g062280 36.768 495 247 12 11 457 9 485 1.28e-91 287
MS.gene055086.t1 MTR_7g063900 40.164 488 235 13 12 457 10 482 2.22e-90 284
MS.gene055086.t1 MTR_7g063820 37.323 493 250 12 6 457 4 478 3.19e-87 276
MS.gene055086.t1 MTR_7g063880 38.365 477 242 13 11 458 10 463 1.53e-86 273
MS.gene055086.t1 MTR_7g063890 38.200 500 253 12 6 464 21 505 1.49e-85 272
MS.gene055086.t1 MTR_7g063840 36.810 489 255 13 1 457 1 467 3.45e-84 268
MS.gene055086.t1 MTR_7g063920 36.441 472 224 10 11 457 10 430 3.04e-82 261
MS.gene055086.t1 MTR_7g063830 36.443 461 244 12 13 457 11 438 3.72e-80 256
MS.gene055086.t1 MTR_3g007740 38.095 462 244 13 11 457 8 442 8.04e-78 250
MS.gene055086.t1 MTR_4g017440 34.556 518 256 15 6 457 2 502 8.37e-78 252
MS.gene055086.t1 MTR_4g019310 33.595 509 259 16 1 453 1 486 3.39e-77 250
MS.gene055086.t1 MTR_4g017830 33.837 529 272 14 1 468 1 512 4.02e-77 251
MS.gene055086.t1 MTR_3g065200 36.831 467 200 14 12 444 3 408 5.36e-76 246
MS.gene055086.t1 MTR_4g019340 34.719 481 250 11 13 446 39 502 3.94e-75 245
MS.gene055086.t1 MTR_4g019340 29.646 226 127 8 12 231 303 502 5.71e-15 77.4
MS.gene055086.t1 MTR_4g017610 33.814 485 252 13 15 454 16 476 1.31e-73 240
MS.gene055086.t1 MTR_7g063670 35.181 469 247 12 13 457 16 451 3.53e-72 236
MS.gene055086.t1 MTR_4g017410 33.333 498 270 13 6 457 3 484 4.54e-71 234
MS.gene055086.t1 MTR_7g066680 44.828 319 109 6 210 465 28 342 4.73e-70 227
MS.gene055086.t1 MTR_7g066680 28.774 212 135 7 8 212 82 284 4.41e-14 73.9
MS.gene055086.t1 MTR_1g014060 54.464 224 91 5 21 242 9 223 8.66e-68 222
MS.gene055086.t1 MTR_1g014060 43.284 201 97 4 279 463 191 390 1.48e-35 136
MS.gene055086.t1 MTR_4g017470 29.831 533 265 11 1 472 1 485 2.00e-59 203
MS.gene055086.t1 MTR_4g017550 31.393 481 239 18 39 455 18 471 6.51e-52 182
MS.gene055086.t1 MTR_4g017550 30.252 238 119 9 5 218 240 454 5.98e-19 89.7
MS.gene055086.t1 MTR_7g066390 53.165 158 46 1 308 465 1 130 3.02e-45 155
MS.gene055086.t1 MTR_7g062270 43.678 174 91 4 13 183 11 180 1.75e-39 147
MS.gene055086.t1 MTR_7g063720 35.784 204 118 3 267 457 23 226 1.61e-34 129
MS.gene055086.t1 MTR_7g063720 27.876 226 134 8 12 236 23 220 4.62e-18 84.0
MS.gene055086.t1 MTR_2g067310 46.243 173 53 4 23 195 58 190 1.62e-34 130
MS.gene055086.t1 MTR_8g023240 24.346 497 294 19 24 475 49 508 8.63e-28 117
MS.gene055086.t1 MTR_7g063850 29.260 311 152 9 184 457 3 282 3.12e-27 111
MS.gene055086.t1 MTR_7g063690 30.035 283 124 7 13 294 13 222 5.76e-27 109
MS.gene055086.t1 MTR_1g115440 33.198 247 131 7 11 241 10 238 6.95e-27 108
MS.gene055086.t1 MTR_7g100420 31.212 330 165 9 164 457 94 397 5.20e-26 110
MS.gene055086.t1 MTR_7g100420 45.055 91 37 3 6 83 26 116 2.08e-12 69.3
MS.gene055086.t1 MTR_8g445860 31.148 244 137 7 12 242 13 238 1.32e-23 99.8
MS.gene055086.t1 MTR_8g445860 30.667 225 113 6 265 450 11 231 7.95e-20 89.0
MS.gene055086.t1 MTR_0150s0010 29.778 225 118 5 265 450 8 231 4.73e-21 92.4
MS.gene055086.t1 MTR_0150s0010 29.921 254 140 7 6 241 4 237 7.22e-20 89.0
MS.gene055086.t1 MTR_1g115455 28.455 246 140 8 12 242 9 233 3.27e-17 81.3
MS.gene055086.t1 MTR_1g115450 45.238 84 41 1 12 95 7 85 4.31e-17 78.6
MS.gene055086.t1 MTR_4g019240 39.344 122 60 5 3 114 4 121 5.71e-17 79.3
MS.gene055086.t1 MTR_4g019330 29.834 181 93 4 281 457 32 182 3.38e-15 74.3
MS.gene055086.t1 MTR_0150s0030 42.353 85 43 2 12 95 8 87 2.14e-14 72.4
MS.gene055086.t1 MTR_8g044880 41.758 91 47 2 12 101 8 93 1.28e-13 71.6
MS.gene055086.t1 MTR_8g445910 43.529 85 42 2 12 95 8 87 1.94e-13 70.9
MS.gene055086.t1 MTR_8g445920 40.000 85 46 1 6 90 4 83 7.61e-13 65.5
MS.gene055086.t1 MTR_8g044890 40.000 85 46 1 6 90 4 83 7.61e-13 65.5
MS.gene055086.t1 MTR_1g115470 40.230 87 47 1 12 98 6 87 3.11e-12 68.9
MS.gene055086.t1 MTR_7g063760 45.455 66 35 1 393 457 2 67 7.56e-12 61.2
MS.gene055086.t1 MTR_1g115435 27.869 244 138 10 12 250 7 217 6.64e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene055086.t1 AT3G09140 32.549 510 282 14 1 461 1 497 4.40e-64 216
MS.gene055086.t1 AT3G09140 31.827 531 286 15 1 461 1 525 8.77e-63 213
MS.gene055086.t1 AT5G01120 28.460 513 306 13 13 479 10 507 4.08e-58 200
MS.gene055086.t1 AT5G43240 30.636 519 291 15 13 479 10 511 6.26e-57 197
MS.gene055086.t1 AT5G43240 30.636 519 291 15 13 479 10 511 6.26e-57 197
MS.gene055086.t1 AT5G43240 30.636 519 291 15 13 479 10 511 6.26e-57 197
MS.gene055086.t1 AT5G01140 30.120 498 284 13 12 479 9 472 7.78e-55 191
MS.gene055086.t1 AT5G01130 30.369 461 281 9 12 457 9 444 1.01e-53 187
MS.gene055086.t1 AT5G01150 29.675 492 285 12 12 460 9 482 5.84e-52 183
MS.gene055086.t1 AT3G09110 32.609 322 208 3 12 332 9 322 2.00e-51 178
MS.gene055086.t1 AT3G09120 32.198 323 197 7 9 321 3 313 7.76e-44 157
MS.gene055086.t1 AT5G37320 26.477 491 300 16 13 460 10 482 2.64e-38 146
MS.gene055086.t1 AT5G43240 28.492 358 200 11 163 479 25 367 6.19e-23 100

Find 87 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATCCTAAATCCCCTATTTC+TGG 0.192592 7.4:+38247838 MS.gene055086:CDS
GCTTCTTAACATTGCCTTTA+GGG 0.201640 7.4:+38246758 MS.gene055086:CDS
TTTCTACCTTTGGCTTCTTC+AGG 0.202952 7.4:-38248907 None:intergenic
TGGAGCACCATTAACGTTTA+TGG 0.217850 7.4:+38247870 MS.gene055086:CDS
CCAGCATATCCACCAGAAAT+AGG 0.239307 7.4:-38247850 None:intergenic
CAAAATTAGCATTAGAGTTT+TGG 0.251042 7.4:+38246669 MS.gene055086:CDS
TGCCATTGACTCCATGCTTC+TGG 0.253037 7.4:-38247206 None:intergenic
CCACCAGAAATAGGGGATTT+AGG 0.261981 7.4:-38247841 None:intergenic
CCAACCTCCAATCCGTTTCT+TGG 0.279458 7.4:-38247457 None:intergenic
CGACTTCTTTACTCAAAATC+TGG 0.292213 7.4:-38247098 None:intergenic
CCTTAATGACGTGGAGAAAA+TGG 0.295092 7.4:+38247966 MS.gene055086:CDS
AGCTTCTTAACATTGCCTTT+AGG 0.310196 7.4:+38246757 MS.gene055086:CDS
CGGCATGCAAAATTGAAAAT+AGG 0.311961 7.4:+38247010 MS.gene055086:CDS
AATGTTGAAGCAGTAAAATT+TGG 0.349258 7.4:+38246811 MS.gene055086:CDS
CAGCATATCCACCAGAAATA+GGG 0.355977 7.4:-38247849 None:intergenic
GATACCTACAGAAAATTATA+AGG 0.356507 7.4:+38247789 MS.gene055086:CDS
CTGTAGGTATCGGTAATATC+TGG 0.356911 7.4:-38247777 None:intergenic
TTCTACCTTTGGCTTCTTCA+GGG 0.358571 7.4:-38248906 None:intergenic
AAACTTGTCATAACCGCATT+TGG 0.359305 7.4:-38247174 None:intergenic
CAATGTCTAATTTCATATTC+CGG 0.365290 7.4:-38246945 None:intergenic
TCAAAATCTGGAACGTTTCT+CGG 0.365833 7.4:-38247086 None:intergenic
TGATCTTCTCAAATTGTCAT+TGG 0.376840 7.4:+38247372 MS.gene055086:CDS
GAGTTCAGATTGAGCGGAAA+TGG 0.387194 7.4:-38247749 None:intergenic
AACGGCCTCAACCAGTTCAT+TGG 0.391379 7.4:+38248148 MS.gene055086:CDS
ATCTTGATCAACAGTATCTA+TGG 0.394065 7.4:+38246866 MS.gene055086:CDS
CCTATTTCTGGTGGATATGC+TGG 0.397124 7.4:+38247850 MS.gene055086:CDS
CAAGAAACGGATTGGAGGTT+GGG 0.405114 7.4:+38247458 MS.gene055086:CDS
TCTGTCACCATAAACGTTAA+TGG 0.410819 7.4:-38247877 None:intergenic
CAACCTCCAATCCGTTTCTT+GGG 0.430506 7.4:-38247456 None:intergenic
AAGATATGAAATACCGTCAA+TGG 0.431308 7.4:-38247926 None:intergenic
AACGGATTGGAGGTTGGGAT+TGG 0.432333 7.4:+38247463 MS.gene055086:CDS
TACTATACTAACCAATGAAC+TGG 0.434085 7.4:-38248159 None:intergenic
AAAGGAAGAATCTAATGAAA+TGG 0.437473 7.4:+38247492 MS.gene055086:CDS
CTCCAGAAGCATGGAGTCAA+TGG 0.448511 7.4:+38247204 MS.gene055086:CDS
TTGGAGGTTGGGATTGGTAG+AGG 0.452268 7.4:+38247469 MS.gene055086:CDS
AATATGCAACACTGCTCCTA+AGG 0.452936 7.4:-38247611 None:intergenic
TTCATATCTTGAAAGAATGA+AGG 0.455227 7.4:+38247939 MS.gene055086:CDS
TCTGTTTGCTGAGGCAGATA+AGG 0.456755 7.4:+38246714 MS.gene055086:CDS
TCAAAATCTGCCTTGACAAA+CGG 0.470384 7.4:+38248130 MS.gene055086:CDS
TTTAGTCCCAAGAAACGGAT+TGG 0.478591 7.4:+38247450 MS.gene055086:CDS
GAGAAAATGGTGGTTGAGAT+TGG 0.482733 7.4:+38247979 MS.gene055086:CDS
TACTCAATCCTATAAACTCA+TGG 0.488738 7.4:+38246911 MS.gene055086:CDS
GAAAATTTAGTCCCAAGAAA+CGG 0.492817 7.4:+38247445 MS.gene055086:CDS
ACACACTCACACACAAACTA+AGG 0.501714 7.4:-38247281 None:intergenic
CCAAGAAACGGATTGGAGGT+TGG 0.507870 7.4:+38247457 MS.gene055086:CDS
CTACCTTTGGCTTCTTCAGG+GGG 0.513478 7.4:-38248904 None:intergenic
TCAGATTGAGCGGAAATGGT+TGG 0.530833 7.4:-38247745 None:intergenic
CTGGTTGAGGCCGTTTGTCA+AGG 0.537345 7.4:-38248140 None:intergenic
ACAGAGGTAGTAGGACTGGA+AGG 0.548379 7.4:-38246980 None:intergenic
TCTACCTTTGGCTTCTTCAG+GGG 0.550005 7.4:-38248905 None:intergenic
ACTCAATCCTATAAACTCAT+GGG 0.552160 7.4:+38246912 MS.gene055086:CDS
TCTCACAGAGGTAGTAGGAC+TGG 0.553733 7.4:-38246984 None:intergenic
ATATGCAACACTGCTCCTAA+GGG 0.554427 7.4:-38247610 None:intergenic
AGCTTACAAAAGAGAACCGT+TGG 0.559569 7.4:-38247636 None:intergenic
AGCATATCCACCAGAAATAG+GGG 0.561344 7.4:-38247848 None:intergenic
AGTCCCAAGAAACGGATTGG+AGG 0.561405 7.4:+38247453 MS.gene055086:CDS
TTAAATCTCCTCCAGAAGCA+TGG 0.561651 7.4:+38247195 MS.gene055086:CDS
ACTAACCAATGAACTGGTTG+AGG 0.567961 7.4:-38248153 None:intergenic
CCTAAATCCCCTATTTCTGG+TGG 0.575624 7.4:+38247841 MS.gene055086:CDS
GGTGGTTGAGATTGGCCAGA+TGG 0.576868 7.4:+38247987 MS.gene055086:CDS
GGTTGGGATTGGTAGAGGAA+AGG 0.578117 7.4:+38247474 MS.gene055086:CDS
AGTCTTGCAATAGTCCCTAA+AGG 0.578435 7.4:-38246772 None:intergenic
CGTGTTCTCACAGAGGTAGT+AGG 0.590180 7.4:-38246989 None:intergenic
ACTCCAATGTCATCCATTGA+CGG 0.602769 7.4:+38247913 MS.gene055086:CDS
CAGTGTTGCATATTCTCCAA+CGG 0.603523 7.4:+38247620 MS.gene055086:CDS
GAAAATCCCTCACCAAAACA+AGG 0.603627 7.4:+38246643 MS.gene055086:CDS
ATACCGTCAATGGATGACAT+TGG 0.606080 7.4:-38247916 None:intergenic
CATTGACTCCATGCTTCTGG+AGG 0.609983 7.4:-38247203 None:intergenic
TTTCCGCTCAATCTGAACTC+AGG 0.610861 7.4:+38247752 MS.gene055086:CDS
GTTTATGGTGACAGATGACT+TGG 0.612440 7.4:+38247885 MS.gene055086:CDS
CTACTACCTCTGTGAGAACA+CGG 0.613030 7.4:+38246990 MS.gene055086:CDS
AAACAAAGTTCTGTTTGCTG+AGG 0.615217 7.4:+38246705 MS.gene055086:CDS
CTCAATCCTATAAACTCATG+GGG 0.615712 7.4:+38246913 MS.gene055086:CDS
CAGAGGTAGTAGGACTGGAA+GGG 0.616285 7.4:-38246979 None:intergenic
TTCACTCTATCAAAGTGTGT+CGG 0.618376 7.4:+38246843 MS.gene055086:CDS
GTTCCTGAGTTCAGATTGAG+CGG 0.623935 7.4:-38247755 None:intergenic
AGGGACTATTGCAAGACTCG+TGG 0.639762 7.4:+38246777 MS.gene055086:CDS
TTAAATATAAAGTCGGACAG+AGG 0.641473 7.4:-38247406 None:intergenic
CAATCCTATAAACTCATGGG+GGG 0.660598 7.4:+38246915 MS.gene055086:CDS
TCAATCCTATAAACTCATGG+GGG 0.663846 7.4:+38246914 MS.gene055086:CDS
GAAGGTTCCCCTTAATGACG+TGG 0.668252 7.4:+38247957 MS.gene055086:CDS
TCTATGGTCAGACACATGCA+AGG 0.670514 7.4:+38246882 MS.gene055086:CDS
TATGCAACACTGCTCCTAAG+GGG 0.682165 7.4:-38247609 None:intergenic
TGCATGCCGTGTTCTCACAG+AGG 0.701708 7.4:-38246996 None:intergenic
GGTTGAGATTGGCCAGATGG+AGG 0.701991 7.4:+38247990 MS.gene055086:CDS
TCTTTCTGTGATGCCAAATG+CGG 0.708786 7.4:+38247161 MS.gene055086:CDS
TAATGACGTGGAGAAAATGG+TGG 0.744537 7.4:+38247969 MS.gene055086:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTAATATAACAATTAAGA+AGG + chr7.4:38248521-38248540 MS.gene055086:intron 10.0%
!! TAAAGATAATATACAAACAA+GGG - chr7.4:38248392-38248411 None:intergenic 15.0%
!!! GAGTATTATAATTTTAATGT+AGG - chr7.4:38248206-38248225 None:intergenic 15.0%
!!! TGTAATTTAAAATGAACAAA+AGG + chr7.4:38248726-38248745 MS.gene055086:intron 15.0%
!!! TTGCTTTTTAAATATAAAGT+CGG - chr7.4:38247416-38247435 None:intergenic 15.0%
!! AAGAATCAAAAATGTATTTG+TGG + chr7.4:38247051-38247070 MS.gene055086:CDS 20.0%
!! CTAAAGATAATATACAAACA+AGG - chr7.4:38248393-38248412 None:intergenic 20.0%
!! CTTGTTTGTATATTATCTTT+AGG + chr7.4:38248391-38248410 MS.gene055086:intron 20.0%
!! TGTACATGAACATATAATAA+AGG - chr7.4:38248838-38248857 None:intergenic 20.0%
!!! AAATTCAACTTTTATCGTAT+CGG + chr7.4:38247134-38247153 MS.gene055086:CDS 20.0%
!!! GTACTACTTGTTATATTAAA+TGG - chr7.4:38248616-38248635 None:intergenic 20.0%
!!! TTATAATTTTCTGTAGGTAT+CGG - chr7.4:38247790-38247809 None:intergenic 20.0%
!!! TTTTCCTTATAATTTTCTGT+AGG - chr7.4:38247796-38247815 None:intergenic 20.0%
! AAAACGTGTTAGTGAATTAA+TGG - chr7.4:38248504-38248523 None:intergenic 25.0%
! AAAGGAAGAATCTAATGAAA+TGG + chr7.4:38247492-38247511 MS.gene055086:CDS 25.0%
! AAGAGATTGACAATCATAAA+GGG + chr7.4:38248758-38248777 MS.gene055086:intron 25.0%
! AATGTTGAAGCAGTAAAATT+TGG + chr7.4:38246811-38246830 MS.gene055086:CDS 25.0%
! AGTTAATATCAGCAAAAAAG+AGG + chr7.4:38247251-38247270 MS.gene055086:CDS 25.0%
! ATTTGTAGAGTGATTGAAAA+TGG + chr7.4:38248329-38248348 MS.gene055086:intron 25.0%
! CAATGTCTAATTTCATATTC+CGG - chr7.4:38246948-38246967 None:intergenic 25.0%
! CTACAAATTCCTTCAAAATT+TGG - chr7.4:38248317-38248336 None:intergenic 25.0%
! GATACCTACAGAAAATTATA+AGG + chr7.4:38247789-38247808 MS.gene055086:CDS 25.0%
! TTCATATCTTGAAAGAATGA+AGG + chr7.4:38247939-38247958 MS.gene055086:CDS 25.0%
!! AGTAGTACTAATTTTCCATA+AGG + chr7.4:38248628-38248647 MS.gene055086:intron 25.0%
!! CAAAATTAGCATTAGAGTTT+TGG + chr7.4:38246669-38246688 MS.gene055086:CDS 25.0%
!!! ATATTATCTTTAGGATTGAC+TGG + chr7.4:38248400-38248419 MS.gene055086:intron 25.0%
!!! TATAATTTTAATGTAGGACG+AGG - chr7.4:38248200-38248219 None:intergenic 25.0%
AAAATGAACAAAAGGTGAGA+TGG + chr7.4:38248734-38248753 MS.gene055086:intron 30.0%
AAATGAACAAAAGGTGAGAT+GGG + chr7.4:38248735-38248754 MS.gene055086:intron 30.0%
AAGATATGAAATACCGTCAA+TGG - chr7.4:38247929-38247948 None:intergenic 30.0%
AATTTGGTCTCTCTAAATGT+TGG - chr7.4:38248301-38248320 None:intergenic 30.0%
ACAAGGGCTAAATATGTTAT+TGG - chr7.4:38248376-38248395 None:intergenic 30.0%
ACTCAATCCTATAAACTCAT+GGG + chr7.4:38246912-38246931 MS.gene055086:CDS 30.0%
ATATACACACAGTTACCTTA+TGG - chr7.4:38248646-38248665 None:intergenic 30.0%
ATCTTGATCAACAGTATCTA+TGG + chr7.4:38246866-38246885 MS.gene055086:CDS 30.0%
CAACCACATGAAATCAAATA+TGG - chr7.4:38248077-38248096 None:intergenic 30.0%
CAGATATGAAAATAGTATGC+AGG - chr7.4:38248431-38248450 None:intergenic 30.0%
GAAAATTTAGTCCCAAGAAA+CGG + chr7.4:38247445-38247464 MS.gene055086:CDS 30.0%
GAAGAGATTGACAATCATAA+AGG + chr7.4:38248757-38248776 MS.gene055086:intron 30.0%
TAAGGTAACTGTGTGTATAT+TGG + chr7.4:38248646-38248665 MS.gene055086:intron 30.0%
TACTATACTAACCAATGAAC+TGG - chr7.4:38248162-38248181 None:intergenic 30.0%
TACTCAATCCTATAAACTCA+TGG + chr7.4:38246911-38246930 MS.gene055086:CDS 30.0%
TATATGTTCATGTACATTGC+AGG + chr7.4:38248842-38248861 MS.gene055086:intron 30.0%
TCATATGATCTAAGGGTAAT+GGG + chr7.4:38248673-38248692 MS.gene055086:intron 30.0%
TGATCTTCTCAAATTGTCAT+TGG + chr7.4:38247372-38247391 MS.gene055086:CDS 30.0%
TGGATGTTCATATGATCTAA+GGG + chr7.4:38248666-38248685 MS.gene055086:intron 30.0%
TTAAATATAAAGTCGGACAG+AGG - chr7.4:38247409-38247428 None:intergenic 30.0%
TTCATATGATCTAAGGGTAA+TGG + chr7.4:38248672-38248691 MS.gene055086:intron 30.0%
TTGGATGTTCATATGATCTA+AGG + chr7.4:38248665-38248684 MS.gene055086:intron 30.0%
! TCACCATATTTGATTTCATG+TGG + chr7.4:38248071-38248090 MS.gene055086:intron 30.0%
! TGAGTTCAGACAAGTATTTT+AGG + chr7.4:38247692-38247711 MS.gene055086:CDS 30.0%
! TTAGAGAGACCAAATTTTGA+AGG + chr7.4:38248305-38248324 MS.gene055086:intron 30.0%
!! AATTAGCATTAGAGTTTTGG+TGG + chr7.4:38246672-38246691 MS.gene055086:CDS 30.0%
!! CAGATTTTGAGTAAAGAAGT+CGG + chr7.4:38247099-38247118 MS.gene055086:CDS 30.0%
!! CTAATTTTGTCACCTTGTTT+TGG - chr7.4:38246658-38246677 None:intergenic 30.0%
!!! TAAAGAAGTCGGTTTTGTTA+AGG + chr7.4:38247110-38247129 MS.gene055086:CDS 30.0%
AAACTTGTCATAACCGCATT+TGG - chr7.4:38247177-38247196 None:intergenic 35.0%
AGCTTCTTAACATTGCCTTT+AGG + chr7.4:38246757-38246776 MS.gene055086:CDS 35.0%
CACACTAATTCTTCAATCTG+TGG + chr7.4:38247335-38247354 MS.gene055086:intron 35.0%
CATATGATCTAAGGGTAATG+GGG + chr7.4:38248674-38248693 MS.gene055086:intron 35.0%
CGACTTCTTTACTCAAAATC+TGG - chr7.4:38247101-38247120 None:intergenic 35.0%
CGGCATGCAAAATTGAAAAT+AGG + chr7.4:38247010-38247029 MS.gene055086:CDS 35.0%
CTCAATCCTATAAACTCATG+GGG + chr7.4:38246913-38246932 MS.gene055086:CDS 35.0%
CTTCAATCTGTGGTTTGAAT+AGG + chr7.4:38247345-38247364 MS.gene055086:intron 35.0%
GAGTGATTGAAAATGGAAGT+TGG + chr7.4:38248336-38248355 MS.gene055086:intron 35.0%
GCATGAGTTGTGAAAATAGT+TGG - chr7.4:38248555-38248574 None:intergenic 35.0%
GCTTCTTAACATTGCCTTTA+GGG + chr7.4:38246758-38246777 MS.gene055086:CDS 35.0%
TCAAAATCTGCCTTGACAAA+CGG + chr7.4:38248130-38248149 MS.gene055086:CDS 35.0%
TCAATCCTATAAACTCATGG+GGG + chr7.4:38246914-38246933 MS.gene055086:CDS 35.0%
TCTGTCACCATAAACGTTAA+TGG - chr7.4:38247880-38247899 None:intergenic 35.0%
TTCACTCTATCAAAGTGTGT+CGG + chr7.4:38246843-38246862 MS.gene055086:CDS 35.0%
! AAACAAAGTTCTGTTTGCTG+AGG + chr7.4:38246705-38246724 MS.gene055086:CDS 35.0%
! ATTTGATTTCATGTGGTTGC+AGG + chr7.4:38248078-38248097 MS.gene055086:intron 35.0%
! ATTTTCTCCACGTCATTAAG+GGG - chr7.4:38247967-38247986 None:intergenic 35.0%
! CATTTTCTCCACGTCATTAA+GGG - chr7.4:38247968-38247987 None:intergenic 35.0%
! TCAAAATCTGGAACGTTTCT+CGG - chr7.4:38247089-38247108 None:intergenic 35.0%
! TTTGATTTCATGTGGTTGCA+GGG + chr7.4:38248079-38248098 MS.gene055086:intron 35.0%
!! GATTTTGTTTGTTGAAGCAG+AGG + chr7.4:38247549-38247568 MS.gene055086:CDS 35.0%
!! TCATAAAGGGTGTGTAGTTT+TGG + chr7.4:38248771-38248790 MS.gene055086:intron 35.0%
!!! AGTTTTTTCACTTTCCCCTT+AGG + chr7.4:38247595-38247614 MS.gene055086:CDS 35.0%
AATATGCAACACTGCTCCTA+AGG - chr7.4:38247614-38247633 None:intergenic 40.0%
ACACACTCACACACAAACTA+AGG - chr7.4:38247284-38247303 None:intergenic 40.0%
ACTAACCAATGAACTGGTTG+AGG - chr7.4:38248156-38248175 None:intergenic 40.0%
ACTCCAATGTCATCCATTGA+CGG + chr7.4:38247913-38247932 MS.gene055086:CDS 40.0%
AGCATATCCACCAGAAATAG+GGG - chr7.4:38247851-38247870 None:intergenic 40.0%
AGCTTACAAAAGAGAACCGT+TGG - chr7.4:38247639-38247658 None:intergenic 40.0%
AGTCTTGCAATAGTCCCTAA+AGG - chr7.4:38246775-38246794 None:intergenic 40.0%
ATACCGTCAATGGATGACAT+TGG - chr7.4:38247919-38247938 None:intergenic 40.0%
ATATGCAACACTGCTCCTAA+GGG - chr7.4:38247613-38247632 None:intergenic 40.0%
ATGTAGGACGAGGTTATGTT+TGG - chr7.4:38248190-38248209 None:intergenic 40.0%
CAATCCTATAAACTCATGGG+GGG + chr7.4:38246915-38246934 MS.gene055086:CDS 40.0%
CAGCATATCCACCAGAAATA+GGG - chr7.4:38247852-38247871 None:intergenic 40.0%
CAGTGTTGCATATTCTCCAA+CGG + chr7.4:38247620-38247639 MS.gene055086:CDS 40.0%
CCTTAATGACGTGGAGAAAA+TGG + chr7.4:38247966-38247985 MS.gene055086:CDS 40.0%
GAAAATCCCTCACCAAAACA+AGG + chr7.4:38246643-38246662 MS.gene055086:CDS 40.0%
GAGAAAATGGTGGTTGAGAT+TGG + chr7.4:38247979-38247998 MS.gene055086:CDS 40.0%
GATCCTAAATCCCCTATTTC+TGG + chr7.4:38247838-38247857 MS.gene055086:CDS 40.0%
GTTTATGGTGACAGATGACT+TGG + chr7.4:38247885-38247904 MS.gene055086:CDS 40.0%
TAATGACGTGGAGAAAATGG+TGG + chr7.4:38247969-38247988 MS.gene055086:CDS 40.0%
TCTTTCTGTGATGCCAAATG+CGG + chr7.4:38247161-38247180 MS.gene055086:CDS 40.0%
TTAAATCTCCTCCAGAAGCA+TGG + chr7.4:38247195-38247214 MS.gene055086:CDS 40.0%
TTTAGTCCCAAGAAACGGAT+TGG + chr7.4:38247450-38247469 MS.gene055086:CDS 40.0%
! CCATTTTCTCCACGTCATTA+AGG - chr7.4:38247969-38247988 None:intergenic 40.0%
! GTTTGTCAAGGCAGATTTTG+AGG - chr7.4:38248131-38248150 None:intergenic 40.0%
! TGGAGCACCATTAACGTTTA+TGG + chr7.4:38247870-38247889 MS.gene055086:CDS 40.0%
! TTCTACCTTTGGCTTCTTCA+GGG - chr7.4:38248909-38248928 None:intergenic 40.0%
! TTTCTACCTTTGGCTTCTTC+AGG - chr7.4:38248910-38248929 None:intergenic 40.0%
!! CTGTAGGTATCGGTAATATC+TGG - chr7.4:38247780-38247799 None:intergenic 40.0%
!!! TTGTCACCTTGTTTTGGTGA+GGG - chr7.4:38246652-38246671 None:intergenic 40.0%
!!! TTTGTCACCTTGTTTTGGTG+AGG - chr7.4:38246653-38246672 None:intergenic 40.0%
ATTGGCCAGATGGAGGTAAA+AGG + chr7.4:38247997-38248016 MS.gene055086:intron 45.0%
CAACCTCCAATCCGTTTCTT+GGG - chr7.4:38247459-38247478 None:intergenic 45.0%
CCACCAGAAATAGGGGATTT+AGG - chr7.4:38247844-38247863 None:intergenic 45.0%
CCAGCATATCCACCAGAAAT+AGG - chr7.4:38247853-38247872 None:intergenic 45.0%
CTACTACCTCTGTGAGAACA+CGG + chr7.4:38246990-38247009 MS.gene055086:CDS 45.0%
GAGTTCAGATTGAGCGGAAA+TGG - chr7.4:38247752-38247771 None:intergenic 45.0%
GTTCCTGAGTTCAGATTGAG+CGG - chr7.4:38247758-38247777 None:intergenic 45.0%
TAAGCCCCCCATGAGTTTAT+AGG - chr7.4:38246922-38246941 None:intergenic 45.0%
TATGCAACACTGCTCCTAAG+GGG - chr7.4:38247612-38247631 None:intergenic 45.0%
TCAGATTGAGCGGAAATGGT+TGG - chr7.4:38247748-38247767 None:intergenic 45.0%
TCTATGGTCAGACACATGCA+AGG + chr7.4:38246882-38246901 MS.gene055086:CDS 45.0%
TTGGCCAGATGGAGGTAAAA+GGG + chr7.4:38247998-38248017 MS.gene055086:intron 45.0%
TTTCCGCTCAATCTGAACTC+AGG + chr7.4:38247752-38247771 MS.gene055086:CDS 45.0%
! TCTACCTTTGGCTTCTTCAG+GGG - chr7.4:38248908-38248927 None:intergenic 45.0%
! TCTGTTTGCTGAGGCAGATA+AGG + chr7.4:38246714-38246733 MS.gene055086:CDS 45.0%
!! CAAGAAACGGATTGGAGGTT+GGG + chr7.4:38247458-38247477 MS.gene055086:CDS 45.0%
!! CCTAAATCCCCTATTTCTGG+TGG + chr7.4:38247841-38247860 MS.gene055086:CDS 45.0%
!! CCTATTTCTGGTGGATATGC+TGG + chr7.4:38247850-38247869 MS.gene055086:CDS 45.0%
!! TCTAAGGGTAATGGGGAGTA+AGG + chr7.4:38248681-38248700 MS.gene055086:intron 45.0%
AACGGCCTCAACCAGTTCAT+TGG + chr7.4:38248148-38248167 MS.gene055086:CDS 50.0%
ACAGAGGTAGTAGGACTGGA+AGG - chr7.4:38246983-38247002 None:intergenic 50.0%
AGGGACTATTGCAAGACTCG+TGG + chr7.4:38246777-38246796 MS.gene055086:CDS 50.0%
CAGAGGTAGTAGGACTGGAA+GGG - chr7.4:38246982-38247001 None:intergenic 50.0%
CCAACCTCCAATCCGTTTCT+TGG - chr7.4:38247460-38247479 None:intergenic 50.0%
CGTGTTCTCACAGAGGTAGT+AGG - chr7.4:38246992-38247011 None:intergenic 50.0%
GAAGGTTCCCCTTAATGACG+TGG + chr7.4:38247957-38247976 MS.gene055086:CDS 50.0%
TCTCACAGAGGTAGTAGGAC+TGG - chr7.4:38246987-38247006 None:intergenic 50.0%
! CACTCCCTTTTACCTCCATC+TGG - chr7.4:38248005-38248024 None:intergenic 50.0%
! CTACCTTTGGCTTCTTCAGG+GGG - chr7.4:38248907-38248926 None:intergenic 50.0%
! CTCCAGAAGCATGGAGTCAA+TGG + chr7.4:38247204-38247223 MS.gene055086:CDS 50.0%
! GGTTGGGATTGGTAGAGGAA+AGG + chr7.4:38247474-38247493 MS.gene055086:CDS 50.0%
! TTGGAGGTTGGGATTGGTAG+AGG + chr7.4:38247469-38247488 MS.gene055086:CDS 50.0%
!! AACGGATTGGAGGTTGGGAT+TGG + chr7.4:38247463-38247482 MS.gene055086:CDS 50.0%
!! AGTCCCAAGAAACGGATTGG+AGG + chr7.4:38247453-38247472 MS.gene055086:CDS 50.0%
!! CATTGACTCCATGCTTCTGG+AGG - chr7.4:38247206-38247225 None:intergenic 50.0%
!! CCAAGAAACGGATTGGAGGT+TGG + chr7.4:38247457-38247476 MS.gene055086:CDS 50.0%
!! TGCCATTGACTCCATGCTTC+TGG - chr7.4:38247209-38247228 None:intergenic 50.0%
!!! GGGTGTGTAGTTTTGGTCAC+TGG + chr7.4:38248778-38248797 MS.gene055086:intron 50.0%
ACTCATGGGGGGCTTATTGC+CGG + chr7.4:38246926-38246945 MS.gene055086:CDS 55.0%
CTGGTTGAGGCCGTTTGTCA+AGG - chr7.4:38248143-38248162 None:intergenic 55.0%
TGCATGCCGTGTTCTCACAG+AGG - chr7.4:38246999-38247018 None:intergenic 55.0%
! GGTGGTTGAGATTGGCCAGA+TGG + chr7.4:38247987-38248006 MS.gene055086:CDS 55.0%
! GGTTGAGATTGGCCAGATGG+AGG + chr7.4:38247990-38248009 MS.gene055086:CDS 55.0%
TCGCCCCCTGAAGAAGCCAA+AGG + chr7.4:38248901-38248920 MS.gene055086:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.4 gene 38246637 38248934 38246637 ID=MS.gene055086
chr7.4 mRNA 38246637 38248934 38246637 ID=MS.gene055086.t1;Parent=MS.gene055086
chr7.4 exon 38246637 38247293 38246637 ID=MS.gene055086.t1.exon1;Parent=MS.gene055086.t1
chr7.4 CDS 38246637 38247293 38246637 ID=cds.MS.gene055086.t1;Parent=MS.gene055086.t1
chr7.4 exon 38247367 38248011 38247367 ID=MS.gene055086.t1.exon2;Parent=MS.gene055086.t1
chr7.4 CDS 38247367 38248011 38247367 ID=cds.MS.gene055086.t1;Parent=MS.gene055086.t1
chr7.4 exon 38248100 38248169 38248100 ID=MS.gene055086.t1.exon3;Parent=MS.gene055086.t1
chr7.4 CDS 38248100 38248169 38248100 ID=cds.MS.gene055086.t1;Parent=MS.gene055086.t1
chr7.4 exon 38248864 38248934 38248864 ID=MS.gene055086.t1.exon4;Parent=MS.gene055086.t1
chr7.4 CDS 38248864 38248934 38248864 ID=cds.MS.gene055086.t1;Parent=MS.gene055086.t1
Gene Sequence

>MS.gene055086

ATGGCTGAAAATCCCTCACCAAAACAAGGTGACAAAATTAGCATTAGAGTTTTGGTGGAAAAGAAGAAAAACAAAGTTCTGTTTGCTGAGGCAGATAAGGATTTTGTTGATGTTCTTTTCAGCTTCTTAACATTGCCTTTAGGGACTATTGCAAGACTCGTGGCTAAAGAGTCAAATGTTGAAGCAGTAAAATTTGGCAGCATTAGTTCACTCTATCAAAGTGTGTCGGATCTTGATCAACAGTATCTATGGTCAGACACATGCAAGGAAATGCTACTCAATCCTATAAACTCATGGGGGGCTTATTGCCGGAATATGAAATTAGACATTGATGATACTGAGCCCTTCCAGTCCTACTACCTCTGTGAGAACACGGCATGCAAAATTGAAAATAGGTACTGTGTGAGTCACTTTAAGAATCAAAAATGTATTTGTGGAAAAATGTTGAACCGAGAAACGTTCCAGATTTTGAGTAAAGAAGTCGGTTTTGTTAAGGAAAATTCAACTTTTATCGTATCGGATGATCTTTCTGTGATGCCAAATGCGGTTATGACAAGTTTAAATCTCCTCCAGAAGCATGGAGTCAATGGCATCAATGCTATTGATAAACAAACAGTTAATATCAGCAAAAAAGAGGCAAGTATCCTTAGTTTGTGTGTGAGTGTGTGTCTTTACTTTTGATGATTATATTGTTTTAACACACTAATTCTTCAATCTGTGGTTTGAATAGGTACTTGATCTTCTCAAATTGTCATTGGTTTCAAAAACTCCTCTGTCCGACTTTATATTTAAAAAGCAACAATTTGTTGAAAATTTAGTCCCAAGAAACGGATTGGAGGTTGGGATTGGTAGAGGAAAGGAAGAATCTAATGAAATGGTTGTGAAAGTACTGAGAAGAAAATCAAATGAACAGATTTTGTTTGTTGAAGCAGAGGAAGATTTTGCTGACTTTGTTTTTAGTTTTTTCACTTTCCCCTTAGGAGCAGTGTTGCATATTCTCCAACGGTTCTCTTTTGTAAGCTGCATTGATAGTTTATATAAGAGTGTGACTGAATTGAGTTCAGACAAGTATTTTAGGTCACAGTTTTTCAAAGACATACTAACTAGTCCAACCATTTCCGCTCAATCTGAACTCAGGAACCAGATATTACCGATACCTACAGAAAATTATAAGGAAAAAAATATGTCATTTGAATTTGTTGATCCTAAATCCCCTATTTCTGGTGGATATGCTGGAGCACCATTAACGTTTATGGTGACAGATGACTTGGTTGTGACTCCAATGTCATCCATTGACGGTATTTCATATCTTGAAAGAATGAAGGTTCCCCTTAATGACGTGGAGAAAATGGTGGTTGAGATTGGCCAGATGGAGGTAAAAGGGAGTGTGTTAGTCTTTCATTCTAATATTTCTTGTATTAGATTCATAACAACTCACCATATTTGATTTCATGTGGTTGCAGGGTCTCAGCATACTTAGAGCTTCATTGACCTCAAAATCTGCCTTGACAAACGGCCTCAACCAGTTCATTGGTTAGTATAGTATTAGTCCAAACATAACCTCGTCCTACATTAAAATTATAATACTCACAATATTAAAGTTCATCGAGAAAATTATTCAAAAATAGTCACTTCGAACATGTTAAGTGAACTTTTGTTAACCAACATTTAGAGAGACCAAATTTTGAAGGAATTTGTAGAGTGATTGAAAATGGAAGTTGGTATATTTAGAGAGACCAATAACATATTTAGCCCTTGTTTGTATATTATCTTTAGGATTGACTGGTTATGCCTGCATACTATTTTCATATCTGAAATACTTGTGTATTGTAAACTAACATTTGTGTATTGTAAACTAACATAGCCATTAATTCACTAACACGTTTTTAATATAACAATTAAGAAGGAGAAATTACCAACTATTTTCACAACTCATGCTTACAAAAATATTAGATGACTACTAATATTAGATGACTCCATTTAATATAACAAGTAGTACTAATTTTCCATAAGGTAACTGTGTGTATATTGGATGTTCATATGATCTAAGGGTAATGGGGAGTAAGGACAGTTAATGTCAAGAAAATTATGTAATTTAAAATGAACAAAAGGTGAGATGGGAAGAGATTGACAATCATAAAGGGTGTGTAGTTTTGGTCACTGGTTTGTTAAATAATTTTGAATATGTACTAACTACTCCTTTATTATATGTTCATGTACATTGCAGGTAGTAGATCTGTTCAGAATAATTTGTCATCTAATGCTCGCCCCCTGAAGAAGCCAAAGGTAGAAAAATGA
Protein sequence

>MS.gene055086.t1

MAENPSPKQGDKISIRVLVEKKKNKVLFAEADKDFVDVLFSFLTLPLGTIARLVAKESNVEAVKFGSISSLYQSVSDLDQQYLWSDTCKEMLLNPINSWGAYCRNMKLDIDDTEPFQSYYLCENTACKIENRYCVSHFKNQKCICGKMLNRETFQILSKEVGFVKENSTFIVSDDLSVMPNAVMTSLNLLQKHGVNGINAIDKQTVNISKKEASILSLCVLDLLKLSLVSKTPLSDFIFKKQQFVENLVPRNGLEVGIGRGKEESNEMVVKVLRRKSNEQILFVEAEEDFADFVFSFFTFPLGAVLHILQRFSFVSCIDSLYKSVTELSSDKYFRSQFFKDILTSPTISAQSELRNQILPIPTENYKEKNMSFEFVDPKSPISGGYAGAPLTFMVTDDLVVTPMSSIDGISYLERMKVPLNDVEKMVVEIGQMEGLSILRASLTSKSALTNGLNQFIGSRSVQNNLSSNARPLKKPKVEK