AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89793

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89793.t1 MTR_4g017440 80.952 399 52 4 2 399 133 508 0.0 622
MS.gene89793.t1 MTR_4g017830 63.433 402 124 6 2 399 125 507 7.27e-170 486
MS.gene89793.t1 MTR_4g017410 63.250 400 110 6 2 399 126 490 4.15e-161 462
MS.gene89793.t1 MTR_4g017410 28.405 257 137 11 151 387 12 241 3.46e-13 71.2
MS.gene89793.t1 MTR_4g017470 65.922 358 111 3 43 399 129 476 6.04e-155 446
MS.gene89793.t1 MTR_4g017470 36.036 111 54 2 151 244 15 125 2.02e-11 65.9
MS.gene89793.t1 MTR_4g019340 62.694 386 100 7 3 383 157 503 6.46e-154 444
MS.gene89793.t1 MTR_4g017550 61.323 393 120 9 3 389 103 469 9.06e-149 430
MS.gene89793.t1 MTR_4g017610 52.051 390 145 7 2 390 128 476 1.45e-120 358
MS.gene89793.t1 MTR_4g017610 33.333 114 61 3 151 249 16 129 9.84e-11 63.5
MS.gene89793.t1 MTR_4g019310 36.253 411 214 11 2 399 122 497 7.45e-68 223
MS.gene89793.t1 MTR_4g019310 28.235 255 143 8 148 386 8 238 5.76e-11 64.3
MS.gene89793.t1 MTR_7g066340 29.877 405 201 11 2 393 119 453 2.93e-41 152
MS.gene89793.t1 MTR_7g066340 28.854 253 133 10 148 386 12 231 2.15e-14 75.1
MS.gene89793.t1 MTR_7g058710 29.327 416 238 12 2 399 106 483 4.63e-40 149
MS.gene89793.t1 MTR_7g058710 25.703 249 146 8 143 386 7 221 3.05e-13 71.6
MS.gene89793.t1 MTR_7g066570 31.051 409 217 12 2 393 120 480 6.00e-39 147
MS.gene89793.t1 MTR_7g066400 32.836 402 186 14 2 389 102 433 2.41e-38 143
MS.gene89793.t1 MTR_7g066430 31.795 390 228 10 11 393 127 485 3.30e-38 144
MS.gene89793.t1 MTR_7g066350 31.671 401 192 11 2 390 116 446 4.75e-38 143
MS.gene89793.t1 MTR_7g066350 27.016 248 144 8 148 387 9 227 8.31e-12 67.0
MS.gene89793.t1 MTR_7g066260 31.931 404 194 14 2 389 120 458 3.19e-36 138
MS.gene89793.t1 MTR_7g066280 29.534 386 221 10 15 393 49 390 1.06e-34 133
MS.gene89793.t1 MTR_7g062280 28.974 390 222 8 16 399 151 491 2.92e-34 133
MS.gene89793.t1 MTR_7g062280 30.290 241 129 10 161 386 23 239 7.92e-12 67.0
MS.gene89793.t1 MTR_1g041315 30.390 385 185 13 9 377 134 451 1.43e-32 128
MS.gene89793.t1 MTR_7g066410 28.372 430 237 12 2 393 116 512 6.73e-32 126
MS.gene89793.t1 MTR_7g063900 29.695 394 238 10 3 393 125 482 2.74e-31 124
MS.gene89793.t1 MTR_7g063900 35.577 104 60 3 148 244 10 113 8.68e-11 63.9
MS.gene89793.t1 MTR_7g063660 28.747 407 237 14 2 399 115 477 8.27e-31 123
MS.gene89793.t1 MTR_7g063660 39.474 114 61 4 148 254 9 121 9.06e-15 76.3
MS.gene89793.t1 MTR_7g062300 28.609 381 213 9 19 392 144 472 2.55e-30 121
MS.gene89793.t1 MTR_7g062300 28.346 254 141 9 143 386 7 229 4.53e-13 70.9
MS.gene89793.t1 MTR_7g063890 30.526 380 221 11 19 393 157 498 2.18e-29 119
MS.gene89793.t1 MTR_7g066580 29.584 409 203 18 2 393 101 441 9.41e-29 117
MS.gene89793.t1 MTR_7g012680 28.537 410 204 11 2 399 119 451 1.53e-27 113
MS.gene89793.t1 MTR_7g063840 27.095 358 204 8 54 399 161 473 3.66e-27 112
MS.gene89793.t1 MTR_7g066290 34.400 250 144 4 2 239 120 361 5.21e-27 112
MS.gene89793.t1 MTR_7g063880 26.277 411 231 14 2 399 117 468 8.09e-26 108
MS.gene89793.t1 MTR_7g063880 35.606 132 75 5 143 267 6 134 5.26e-13 70.9
MS.gene89793.t1 MTR_7g063920 27.350 351 206 8 55 399 129 436 1.84e-25 107
MS.gene89793.t1 MTR_7g066360 30.925 346 169 10 49 389 165 445 1.65e-23 102
MS.gene89793.t1 MTR_7g063820 29.248 359 199 12 55 399 167 484 1.05e-22 100
MS.gene89793.t1 MTR_7g063820 37.383 107 60 3 149 249 11 116 7.28e-12 67.0
MS.gene89793.t1 MTR_7g066680 32.143 280 154 8 99 372 37 286 1.62e-22 97.8
MS.gene89793.t1 MTR_7g066310 31.707 246 113 5 148 393 193 383 2.06e-22 98.6
MS.gene89793.t1 MTR_7g063670 28.011 357 195 9 49 399 157 457 2.24e-22 99.0
MS.gene89793.t1 MTR_7g058700 32.365 241 105 9 15 231 632 838 1.44e-20 94.4
MS.gene89793.t1 MTR_3g065200 25.781 384 204 11 9 388 110 416 5.51e-20 91.7
MS.gene89793.t1 MTR_8g445860 27.935 247 152 5 144 387 9 232 8.51e-20 88.2
MS.gene89793.t1 MTR_7g063830 26.870 361 184 12 46 399 157 444 1.09e-18 87.8
MS.gene89793.t1 MTR_7g063830 28.216 241 131 11 161 391 23 231 1.09e-12 69.7
MS.gene89793.t1 MTR_3g007740 30.723 166 114 1 54 218 149 314 5.83e-17 82.8
MS.gene89793.t1 MTR_7g066480 30.882 204 119 5 55 248 166 357 2.32e-16 80.9
MS.gene89793.t1 MTR_7g100420 27.920 351 198 12 52 394 95 398 1.49e-14 75.1
MS.gene89793.t1 MTR_1g115455 28.740 254 142 9 143 387 4 227 2.63e-14 72.4
MS.gene89793.t1 MTR_4g019350 40.187 107 41 1 206 312 1 84 3.05e-14 68.2
MS.gene89793.t1 MTR_1g014060 29.435 248 117 7 157 399 196 390 1.06e-13 72.4
MS.gene89793.t1 MTR_0150s0010 26.295 251 156 7 143 387 5 232 2.15e-13 69.7
MS.gene89793.t1 MTR_0150s0030 34.906 106 60 3 143 245 3 102 2.27e-13 68.9
MS.gene89793.t1 MTR_1g115450 39.535 86 50 1 143 226 2 87 2.58e-13 67.4
MS.gene89793.t1 MTR_8g445910 35.000 120 67 4 143 257 3 116 3.19e-13 69.7
MS.gene89793.t1 MTR_7g066490 28.283 198 119 4 55 248 165 343 4.62e-13 70.5
MS.gene89793.t1 MTR_4g019240 67.347 49 16 0 351 399 112 160 7.90e-13 67.0
MS.gene89793.t1 MTR_8g044880 35.000 120 67 4 143 257 3 116 3.27e-12 67.0
MS.gene89793.t1 MTR_4g019330 41.304 92 52 2 310 399 97 188 8.34e-12 64.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89793.t1 AT5G01120 29.630 405 230 13 1 389 120 485 1.27e-37 142
MS.gene89793.t1 AT5G01140 31.429 420 202 19 1 399 104 458 1.38e-35 136
MS.gene89793.t1 AT5G43240 28.155 412 235 12 2 389 115 489 1.32e-34 134
MS.gene89793.t1 AT5G43240 28.155 412 235 12 2 389 115 489 1.32e-34 134
MS.gene89793.t1 AT5G43240 28.155 412 235 12 2 389 115 489 1.32e-34 134
MS.gene89793.t1 AT3G09140 30.952 420 221 18 2 393 115 493 3.21e-33 130
MS.gene89793.t1 AT3G09140 26.190 252 166 10 143 386 4 243 2.27e-12 68.9
MS.gene89793.t1 AT5G43240 28.912 377 214 10 30 389 6 345 6.59e-33 127
MS.gene89793.t1 AT5G01130 28.536 403 207 14 7 399 120 451 4.48e-31 123
MS.gene89793.t1 AT3G09140 29.018 448 221 19 2 393 115 521 1.23e-28 117
MS.gene89793.t1 AT5G01150 28.218 404 237 11 3 393 116 479 2.19e-28 116
MS.gene89793.t1 AT3G09110 28.899 218 131 6 2 209 115 318 4.21e-20 90.9
MS.gene89793.t1 AT5G37320 28.291 357 197 14 48 393 171 479 7.10e-18 85.9
MS.gene89793.t1 AT3G09120 26.316 209 142 4 1 202 110 313 1.24e-14 74.7

Find 102 sgRNAs with CRISPR-Local

Find 155 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGACATGTTCGGAGATTT+TGG 0.150642 4.4:+83395925 None:intergenic
CCCACTTGTCAAGGTAAATT+TGG 0.181828 4.4:+83395622 None:intergenic
AACAAAGAGACCTCGAAATT+TGG 0.196356 4.4:-83397468 MS.gene89793:CDS
CCTGGCGCAGCACCACATTT+TGG 0.210743 4.4:-83396180 MS.gene89793:CDS
ACCATCTGAAAGCTTTAAAA+TGG 0.212595 4.4:+83396296 None:intergenic
CTTGCGCCTGTTGAAGGAAT+TGG 0.237644 4.4:-83397321 MS.gene89793:CDS
TTAATTCTTTCAAATATAAA+AGG 0.238698 4.4:+83395815 None:intergenic
GGTACTGGTGTACCAAATTT+TGG 0.258982 4.4:-83396099 MS.gene89793:CDS
CACAAGTGGCGGCACTAAAA+TGG 0.259934 4.4:-83396424 MS.gene89793:CDS
TGGTTTGGACAACCAAAATT+TGG 0.263143 4.4:+83396087 None:intergenic
AATCAGAAATGTTGAGGAAT+TGG 0.275365 4.4:+83396067 None:intergenic
GAGGTCTTTCTTAGAGAAAA+TGG 0.295488 4.4:-83397401 MS.gene89793:CDS
CTGCCCACTACATATTATTA+TGG 0.306674 4.4:-83396039 MS.gene89793:CDS
AAGTGGGCTGGGAAACCTTT+TGG 0.309378 4.4:-83395606 MS.gene89793:CDS
CCGCCCACTACATATTATTA+TGG 0.322722 4.4:-83396120 MS.gene89793:CDS
AGGCAGGTTGCCTTTGTACT+TGG 0.329588 4.4:+83396482 None:intergenic
AGCTTCCTTACCATGCCATT+AGG 0.332201 4.4:-83396321 MS.gene89793:CDS
TTCAAAATTGTATTTGTCAT+AGG 0.339563 4.4:+83396513 None:intergenic
GCTTCCTTACCATGCCATTA+GGG 0.353748 4.4:-83396320 MS.gene89793:CDS
GTGCAGAGATTAGCATTATA+CGG 0.353949 4.4:-83395883 MS.gene89793:CDS
CTCTAACGGCTGATGAAGAT+TGG 0.361391 4.4:+83396457 None:intergenic
TCAGATGGTAACTCGTTTGC+TGG 0.365227 4.4:-83396282 MS.gene89793:CDS
GGAATGTTGAGGGGCTGGTT+TGG 0.384748 4.4:+83396153 None:intergenic
GACTACCTTGCGCCTGTTGA+AGG 0.390286 4.4:-83397327 MS.gene89793:CDS
AAATTTGGATTCTGAGGGTC+AGG 0.390940 4.4:-83397453 MS.gene89793:CDS
AAATTTCGAGGTCTCTTTGT+TGG 0.405962 4.4:+83397470 None:intergenic
TTGCGCCTGTTGAAGGAATT+GGG 0.408613 4.4:-83397320 MS.gene89793:CDS
AACAGGCGCAAGGTAGTCAT+AGG 0.418300 4.4:+83397332 None:intergenic
GAAATGTTGAGGAATTGGTT+TGG 0.428433 4.4:+83396072 None:intergenic
CCAAAATGTGGTGCTGCGCC+AGG 0.440390 4.4:+83396180 None:intergenic
CTCCACTTGAAGATCTTCAA+TGG 0.442005 4.4:+83395786 None:intergenic
TCCAAATTTACCTTGACAAG+TGG 0.443877 4.4:-83395623 MS.gene89793:CDS
CAGAACCTTGGTTGCACTTG+TGG 0.445824 4.4:-83397497 MS.gene89793:CDS
CCACCTTCCCTTGACATGTT+CGG 0.449279 4.4:+83395916 None:intergenic
CCCTCAGAATCCAAATTTCG+AGG 0.452176 4.4:+83397458 None:intergenic
CGCACTGTGTTACTCAATCC+TGG 0.461909 4.4:-83396198 MS.gene89793:CDS
CGGCACTAAAATGGATGTTA+AGG 0.464791 4.4:-83396415 MS.gene89793:CDS
AATAACCCAATTCCTTCAAC+AGG 0.469036 4.4:+83397315 None:intergenic
GTGTAGGCTCTGAGCATCCT+TGG 0.469524 4.4:-83395659 MS.gene89793:intron
GTACAAGAGTGTGGAGAACT+TGG 0.471755 4.4:-83396244 MS.gene89793:CDS
TTTACCTTGACAAGTGGGCT+GGG 0.472395 4.4:-83395617 MS.gene89793:CDS
ATTTACCTTGACAAGTGGGC+TGG 0.473382 4.4:-83395618 MS.gene89793:CDS
ACTACATATTATTATGGTAC+TGG 0.476342 4.4:-83396114 MS.gene89793:CDS
ACCTCGAAATTTGGATTCTG+AGG 0.476612 4.4:-83397459 MS.gene89793:CDS
CCTTCCTTGATATATTTATG+TGG 0.478610 4.4:+83396006 None:intergenic
AATCAGGAATGTTGAGGGGC+TGG 0.483938 4.4:+83396148 None:intergenic
TTCACTACTATTCAGAACCT+TGG 0.487641 4.4:-83397509 MS.gene89793:CDS
TCCACTTGAAGATCTTCAAT+GGG 0.491617 4.4:+83395787 None:intergenic
AGCTTTAAAATGGACCCTAA+TGG 0.492701 4.4:+83396306 None:intergenic
AAGGTAGTCATAGGGGAACT+TGG 0.501027 4.4:+83397341 None:intergenic
AGGTTAAGCTATACTTGAGT+AGG 0.508695 4.4:+83396544 None:intergenic
CTTGTTTAGGAAGATCCAAA+AGG 0.511212 4.4:+83395591 None:intergenic
AACTCGTTTGCTGGATGTGT+TGG 0.520836 4.4:-83396273 MS.gene89793:CDS
CAAAATCTCCGAACATGTCA+AGG 0.523947 4.4:-83395924 MS.gene89793:CDS
ACTACATATTATTATGGTAC+AGG 0.525179 4.4:-83396033 MS.gene89793:CDS
CATGTCAAGGGAAGGTGGTG+TGG 0.534164 4.4:-83395911 MS.gene89793:CDS
CTGATGAAGATTGGTTAGGC+AGG 0.539982 4.4:+83396466 None:intergenic
TATACCACATAAATATATCA+AGG 0.540609 4.4:-83396010 MS.gene89793:CDS
ACGGCTGATGAAGATTGGTT+AGG 0.542485 4.4:+83396462 None:intergenic
ACAGGCGCAAGGTAGTCATA+GGG 0.544912 4.4:+83397333 None:intergenic
TGGCACAATCTTCAAATCAT+CGG 0.556682 4.4:+83397361 None:intergenic
CTTGAAGATCTTCAATGGGA+AGG 0.557870 4.4:+83395791 None:intergenic
ATGTCAAGGGAAGGTGGTGT+GGG 0.560436 4.4:-83395910 MS.gene89793:CDS
CAAGTGGAGGTGATAAGCAT+TGG 0.573003 4.4:-83395772 MS.gene89793:CDS
TTCAGATTTGACTCAACTAG+AGG 0.573354 4.4:-83397294 MS.gene89793:CDS
AGAGTAAAACCTTGCACAAG+TGG 0.576824 4.4:-83396438 MS.gene89793:CDS
AGATTAGCATTATACGGTGT+AGG 0.576913 4.4:-83395877 MS.gene89793:CDS
CCACATAAATATATCAAGGA+AGG 0.577989 4.4:-83396006 MS.gene89793:CDS
TGGACCCTAATGGCATGGTA+AGG 0.578120 4.4:+83396316 None:intergenic
TCAGGGTAATAGTACTAGTG+TGG 0.581620 4.4:-83397435 MS.gene89793:CDS
TGGTAATTTGTACAAGAGTG+TGG 0.582155 4.4:-83396253 MS.gene89793:CDS
GTTTCCCAGCCCACTTGTCA+AGG 0.584219 4.4:+83395613 None:intergenic
CCAAATTTACCTTGACAAGT+GGG 0.586402 4.4:-83395622 MS.gene89793:CDS
TTAGTGCCGCCACTTGTGCA+AGG 0.589354 4.4:+83396429 None:intergenic
TAAAATGGACCCTAATGGCA+TGG 0.595393 4.4:+83396311 None:intergenic
TCCCATTGAAGATCTTCAAG+TGG 0.597466 4.4:-83395788 MS.gene89793:CDS
CCTCGAAATTTGGATTCTGA+GGG 0.599214 4.4:-83397458 MS.gene89793:CDS
TAGTGTGGATGCTCAAAATG+AGG 0.602519 4.4:-83397420 MS.gene89793:CDS
CCGAACATGTCAAGGGAAGG+TGG 0.605703 4.4:-83395916 MS.gene89793:CDS
CATTGAAGATCTTCAAGTGG+AGG 0.606584 4.4:-83395785 MS.gene89793:CDS
GAAGGAAACAAAATCACCGT+TGG 0.609986 4.4:+83396350 None:intergenic
GAGGAAATCACACACAACAT+TGG 0.612797 4.4:-83397275 MS.gene89793:CDS
GTACCATAATAATATGTAGT+GGG 0.617649 4.4:+83396117 None:intergenic
CAATTCCTTCAACAGGCGCA+AGG 0.619456 4.4:+83397322 None:intergenic
GTACCATAATAATATGTAGT+GGG 0.621257 4.4:+83396036 None:intergenic
AAAATCTCCGAACATGTCAA+GGG 0.622631 4.4:-83395923 MS.gene89793:CDS
TGTACCATAATAATATGTAG+TGG 0.631710 4.4:+83396035 None:intergenic
TCTCCGAACATGTCAAGGGA+AGG 0.632004 4.4:-83395919 MS.gene89793:CDS
AAAAGTAGCTCCAAGTACAA+AGG 0.632301 4.4:-83396492 MS.gene89793:CDS
ACACAACATTGGCAAGCAAG+AGG 0.636713 4.4:-83397264 MS.gene89793:intron
AGTACCATAATAATATGTAG+TGG 0.637800 4.4:+83396116 None:intergenic
AGAACTTGGACTCTTCATTG+TGG 0.642932 4.4:-83396230 MS.gene89793:CDS
TTTAAATCAGGAATGTTGAG+GGG 0.644292 4.4:+83396144 None:intergenic
ATTTGTCATAGGCTCGTGAG+AGG 0.647045 4.4:+83396524 None:intergenic
ATCATCTTTGTAGAAGCCAA+CGG 0.648266 4.4:-83396366 MS.gene89793:CDS
GTAAAACCTTGCACAAGTGG+CGG 0.651940 4.4:-83396435 MS.gene89793:CDS
GATAAGCATTGGTGAAGCCG+AGG 0.652499 4.4:-83395761 MS.gene89793:intron
CAGGCGCAAGGTAGTCATAG+GGG 0.659750 4.4:+83397334 None:intergenic
CCATAATAATATGTAGTGGG+CGG 0.667025 4.4:+83396120 None:intergenic
AATTTGGATTCTGAGGGTCA+GGG 0.676851 4.4:-83397452 MS.gene89793:CDS
TGGTTTGGACAACCAAAATG+TGG 0.677088 4.4:+83396168 None:intergenic
TGGACGTCAAGAATGCTCCA+AGG 0.688603 4.4:+83395642 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TATATATAATTTGAAGAAAA+AGG - chr4.4:83396370-83396389 MS.gene89793:CDS 10.0%
!! TTAATTCTTTCAAATATAAA+AGG + chr4.4:83397313-83397332 None:intergenic 10.0%
!!! TGTTTATTTTACAAAAATAA+AGG + chr4.4:83396021-83396040 None:intergenic 10.0%
!! AAAAAAAAACTAGATGAAAA+AGG + chr4.4:83396481-83396500 None:intergenic 15.0%
!! TTAAAATATAGGGAAAAATT+CGG + chr4.4:83396118-83396137 None:intergenic 15.0%
!!! ACATTTAATTGATATTTTGA+AGG - chr4.4:83396161-83396180 MS.gene89793:CDS 15.0%
!!! CATTTAATTGATATTTTGAA+GGG - chr4.4:83396162-83396181 MS.gene89793:CDS 15.0%
!! TAAAATATAGGGAAAAATTC+GGG + chr4.4:83396117-83396136 None:intergenic 20.0%
!! TATACCACATAAATATATCA+AGG - chr4.4:83397115-83397134 MS.gene89793:intron 20.0%
!! TTCAAAATTGTATTTGTCAT+AGG + chr4.4:83396615-83396634 None:intergenic 20.0%
!!! TATTGTCATTTGTTTACATT+GGG + chr4.4:83396248-83396267 None:intergenic 20.0%
!!! TTATTGTCATTTGTTTACAT+TGG + chr4.4:83396249-83396268 None:intergenic 20.0%
! AAATATCAATTAAATGTCGC+TGG + chr4.4:83396158-83396177 None:intergenic 25.0%
! ACTACATATTATTATGGTAC+AGG - chr4.4:83397011-83397030 MS.gene89793:intron 25.0%
! ACTACATATTATTATGGTAC+TGG - chr4.4:83397011-83397030 MS.gene89793:intron 25.0%
! AGTACCATAATAATATGTAG+TGG + chr4.4:83397012-83397031 None:intergenic 25.0%
! ATATGCAAGTAAAAGGTTAT+GGG + chr4.4:83395928-83395947 None:intergenic 25.0%
! CAATATTATAATCACAACTG+TGG + chr4.4:83396051-83396070 None:intergenic 25.0%
! GTACCATAATAATATGTAGT+GGG + chr4.4:83397011-83397030 None:intergenic 25.0%
! TAAGATCATATGCAAGTAAA+AGG + chr4.4:83395935-83395954 None:intergenic 25.0%
! TGTACCATAATAATATGTAG+TGG + chr4.4:83397093-83397112 None:intergenic 25.0%
!! CAAGAGTTTTGCATTAAAAA+TGG + chr4.4:83397168-83397187 None:intergenic 25.0%
!! TTTTAAATCAGGAATGTTGA+GGG + chr4.4:83396985-83397004 None:intergenic 25.0%
!!! AGAAAATGGTTCAACTTTTT+TGG - chr4.4:83395738-83395757 MS.gene89793:intron 25.0%
!!! GTTTTAAATCAGAAATGTTG+AGG + chr4.4:83397067-83397086 None:intergenic 25.0%
AAACATGATAGAATAAGCAG+AGG - chr4.4:83396336-83396355 MS.gene89793:CDS 30.0%
AAATAATAACCACCTTACCT+CGG + chr4.4:83397384-83397403 None:intergenic 30.0%
AATCAGAAATGTTGAGGAAT+TGG + chr4.4:83397061-83397080 None:intergenic 30.0%
AATGAATAAGATGTGCAACA+TGG + chr4.4:83396397-83396416 None:intergenic 30.0%
ACAAGCAAAAGGAATCAAAA+AGG + chr4.4:83397445-83397464 None:intergenic 30.0%
ACTAACTGATGTATCCTAAA+GGG + chr4.4:83396086-83396105 None:intergenic 30.0%
CATATGCAAGTAAAAGGTTA+TGG + chr4.4:83395929-83395948 None:intergenic 30.0%
CCACATAAATATATCAAGGA+AGG - chr4.4:83397119-83397138 MS.gene89793:intron 30.0%
CCTTCCTTGATATATTTATG+TGG + chr4.4:83397122-83397141 None:intergenic 30.0%
GTTGCACATCTTATTCATTT+GGG - chr4.4:83396398-83396417 MS.gene89793:CDS 30.0%
TATGCAAGTAAAAGGTTATG+GGG + chr4.4:83395927-83395946 None:intergenic 30.0%
TGTTGCACATCTTATTCATT+TGG - chr4.4:83396397-83396416 MS.gene89793:CDS 30.0%
TTTAAATCAGGAATGTTGAG+GGG + chr4.4:83396984-83397003 None:intergenic 30.0%
! CAATTTTCCAATTCTTCTCT+TGG - chr4.4:83396505-83396524 MS.gene89793:CDS 30.0%
! TTATTGTCATTTGCTTGCTT+TGG + chr4.4:83396305-83396324 None:intergenic 30.0%
!! AATTGATATTTTGAAGGGCA+TGG - chr4.4:83396167-83396186 MS.gene89793:CDS 30.0%
!! ACCATCTGAAAGCTTTAAAA+TGG + chr4.4:83396832-83396851 None:intergenic 30.0%
!! GTTTTAAATCAGGAATGTTG+AGG + chr4.4:83396986-83397005 None:intergenic 30.0%
!! TTACATTGGGTTGAAATACA+AGG + chr4.4:83396235-83396254 None:intergenic 30.0%
!! TTTTTGATTCCTTTTGCTTG+TGG - chr4.4:83397444-83397463 MS.gene89793:CDS 30.0%
!!! TCCATTTTAAAGCTTTCAGA+TGG - chr4.4:83396828-83396847 MS.gene89793:intron 30.0%
AAAAGTAGCTCCAAGTACAA+AGG - chr4.4:83396633-83396652 MS.gene89793:intron 35.0%
AAAATCTCCGAACATGTCAA+GGG - chr4.4:83397202-83397221 MS.gene89793:intron 35.0%
AAATTTCGAGGTCTCTTTGT+TGG + chr4.4:83395658-83395677 None:intergenic 35.0%
AACAAAGAGACCTCGAAATT+TGG - chr4.4:83395657-83395676 MS.gene89793:intron 35.0%
AATAACCCAATTCCTTCAAC+AGG + chr4.4:83395813-83395832 None:intergenic 35.0%
AGAAAGAGTAGAAACATGCT+AGG - chr4.4:83396268-83396287 MS.gene89793:CDS 35.0%
AGATTAGCATTATACGGTGT+AGG - chr4.4:83397248-83397267 MS.gene89793:intron 35.0%
AGGTTAAGCTATACTTGAGT+AGG + chr4.4:83396584-83396603 None:intergenic 35.0%
ATCATCTTTGTAGAAGCCAA+CGG - chr4.4:83396759-83396778 MS.gene89793:intron 35.0%
ATGCAAGTAAAAGGTTATGG+GGG + chr4.4:83395926-83395945 None:intergenic 35.0%
CACTAACTGATGTATCCTAA+AGG + chr4.4:83396087-83396106 None:intergenic 35.0%
CCAAATTTACCTTGACAAGT+GGG - chr4.4:83397503-83397522 MS.gene89793:CDS 35.0%
CTGCCCACTACATATTATTA+TGG - chr4.4:83397086-83397105 MS.gene89793:intron 35.0%
CTTGTTTAGGAAGATCCAAA+AGG + chr4.4:83397537-83397556 None:intergenic 35.0%
GAAATGTTGAGGAATTGGTT+TGG + chr4.4:83397056-83397075 None:intergenic 35.0%
GAGGTCTTTCTTAGAGAAAA+TGG - chr4.4:83395724-83395743 MS.gene89793:intron 35.0%
GGGAAAAACTTGAAATCCTT+GGG - chr4.4:83396418-83396437 MS.gene89793:CDS 35.0%
GGTAAGGAAGCTAAAAATGA+AGG + chr4.4:83396796-83396815 None:intergenic 35.0%
GTGCAGAGATTAGCATTATA+CGG - chr4.4:83397242-83397261 MS.gene89793:intron 35.0%
TCCAAATTTACCTTGACAAG+TGG - chr4.4:83397502-83397521 MS.gene89793:CDS 35.0%
TCCACTTGAAGATCTTCAAT+GGG + chr4.4:83397341-83397360 None:intergenic 35.0%
TGGCACAATCTTCAAATCAT+CGG + chr4.4:83395767-83395786 None:intergenic 35.0%
TGGGAAAAACTTGAAATCCT+TGG - chr4.4:83396417-83396436 MS.gene89793:CDS 35.0%
TTCACTACTATTCAGAACCT+TGG - chr4.4:83395616-83395635 MS.gene89793:CDS 35.0%
TTCAGATTTGACTCAACTAG+AGG - chr4.4:83395831-83395850 MS.gene89793:CDS 35.0%
! CCATAATAATATGTAGTGGG+CGG + chr4.4:83397008-83397027 None:intergenic 35.0%
! TGGTAATTTGTACAAGAGTG+TGG - chr4.4:83396872-83396891 MS.gene89793:intron 35.0%
! TGGTTTGGACAACCAAAATT+TGG + chr4.4:83397041-83397060 None:intergenic 35.0%
! TGTGCAAGGTTTTACTCTAA+CGG + chr4.4:83396685-83396704 None:intergenic 35.0%
!! AGCTTTAAAATGGACCCTAA+TGG + chr4.4:83396822-83396841 None:intergenic 35.0%
ACCTCGAAATTTGGATTCTG+AGG - chr4.4:83395666-83395685 MS.gene89793:intron 40.0%
ACTTGAAATCCTTGGGTGAA+TGG - chr4.4:83396425-83396444 MS.gene89793:CDS 40.0%
AGAACTTGGACTCTTCATTG+TGG - chr4.4:83396895-83396914 MS.gene89793:intron 40.0%
AGAGTAAAACCTTGCACAAG+TGG - chr4.4:83396687-83396706 MS.gene89793:intron 40.0%
CAAAATCTCCGAACATGTCA+AGG - chr4.4:83397201-83397220 MS.gene89793:intron 40.0%
CATTGAAGATCTTCAAGTGG+AGG - chr4.4:83397340-83397359 MS.gene89793:CDS 40.0%
CCCACTTGTCAAGGTAAATT+TGG + chr4.4:83397506-83397525 None:intergenic 40.0%
CCGCCCACTACATATTATTA+TGG - chr4.4:83397005-83397024 MS.gene89793:intron 40.0%
CCTCGAAATTTGGATTCTGA+GGG - chr4.4:83395667-83395686 MS.gene89793:intron 40.0%
CGGCACTAAAATGGATGTTA+AGG - chr4.4:83396710-83396729 MS.gene89793:intron 40.0%
CTCCACTTGAAGATCTTCAA+TGG + chr4.4:83397342-83397361 None:intergenic 40.0%
CTTGAAGATCTTCAATGGGA+AGG + chr4.4:83397337-83397356 None:intergenic 40.0%
GAACTAGCCAAGAGAAGAAT+TGG + chr4.4:83396515-83396534 None:intergenic 40.0%
GAAGGAAACAAAATCACCGT+TGG + chr4.4:83396778-83396797 None:intergenic 40.0%
GAGGAAATCACACACAACAT+TGG - chr4.4:83395850-83395869 MS.gene89793:CDS 40.0%
TAAAATGGACCCTAATGGCA+TGG + chr4.4:83396817-83396836 None:intergenic 40.0%
TAGAAGAAACCATTCACCCA+AGG + chr4.4:83396437-83396456 None:intergenic 40.0%
TAGTGTGGATGCTCAAAATG+AGG - chr4.4:83395705-83395724 MS.gene89793:intron 40.0%
TCAGGGTAATAGTACTAGTG+TGG - chr4.4:83395690-83395709 MS.gene89793:intron 40.0%
TCCCATTGAAGATCTTCAAG+TGG - chr4.4:83397337-83397356 MS.gene89793:CDS 40.0%
! AAATTTGGATTCTGAGGGTC+AGG - chr4.4:83395672-83395691 MS.gene89793:intron 40.0%
! AATTTGGATTCTGAGGGTCA+GGG - chr4.4:83395673-83395692 MS.gene89793:intron 40.0%
! ATTCCTTTTGCTTGTGGTGT+AGG - chr4.4:83397450-83397469 MS.gene89793:CDS 40.0%
! CTTGACATGTTCGGAGATTT+TGG + chr4.4:83397203-83397222 None:intergenic 40.0%
! TGGTTTGGACAACCAAAATG+TGG + chr4.4:83396960-83396979 None:intergenic 40.0%
!! AACCGCAACTTTAAAGCATG+AGG + chr4.4:83395989-83396008 None:intergenic 40.0%
!! CACCTCATGCTTTAAAGTTG+CGG - chr4.4:83395984-83396003 MS.gene89793:CDS 40.0%
!!! GGTACTGGTGTACCAAATTT+TGG - chr4.4:83397026-83397045 MS.gene89793:intron 40.0%
AAGGTAGTCATAGGGGAACT+TGG + chr4.4:83395787-83395806 None:intergenic 45.0%
ACACAACATTGGCAAGCAAG+AGG - chr4.4:83395861-83395880 MS.gene89793:CDS 45.0%
AGCTTCCTTACCATGCCATT+AGG - chr4.4:83396804-83396823 MS.gene89793:intron 45.0%
ATTTACCTTGACAAGTGGGC+TGG - chr4.4:83397507-83397526 MS.gene89793:CDS 45.0%
ATTTGTCATAGGCTCGTGAG+AGG + chr4.4:83396604-83396623 None:intergenic 45.0%
CAAGTGGAGGTGATAAGCAT+TGG - chr4.4:83397353-83397372 MS.gene89793:CDS 45.0%
CCCTCAGAATCCAAATTTCG+AGG + chr4.4:83395670-83395689 None:intergenic 45.0%
CTCTAACGGCTGATGAAGAT+TGG + chr4.4:83396671-83396690 None:intergenic 45.0%
GCTTCCTTACCATGCCATTA+GGG - chr4.4:83396805-83396824 MS.gene89793:intron 45.0%
GTAAAACCTTGCACAAGTGG+CGG - chr4.4:83396690-83396709 MS.gene89793:intron 45.0%
TCAGATGGTAACTCGTTTGC+TGG - chr4.4:83396843-83396862 MS.gene89793:intron 45.0%
TGCAGTATGCTTCACCCTTT+AGG - chr4.4:83396069-83396088 MS.gene89793:CDS 45.0%
TTGCGCCTGTTGAAGGAATT+GGG - chr4.4:83395805-83395824 MS.gene89793:CDS 45.0%
! AACTCGTTTGCTGGATGTGT+TGG - chr4.4:83396852-83396871 MS.gene89793:intron 45.0%
! ACGGCTGATGAAGATTGGTT+AGG + chr4.4:83396666-83396685 None:intergenic 45.0%
! CTGATGAAGATTGGTTAGGC+AGG + chr4.4:83396662-83396681 None:intergenic 45.0%
! GTACAAGAGTGTGGAGAACT+TGG - chr4.4:83396881-83396900 MS.gene89793:intron 45.0%
! GTTTTCCACAAGTGCAACCA+AGG + chr4.4:83395636-83395655 None:intergenic 45.0%
! TTTACCTTGACAAGTGGGCT+GGG - chr4.4:83397508-83397527 MS.gene89793:CDS 45.0%
!!! GTAGTGGGCGGTTTTAAATC+AGG + chr4.4:83396996-83397015 None:intergenic 45.0%
!!! AAATTTGAAATTTAAAATAT+AGG + chr4.4:83396129-83396148 None:intergenic 5.0%
!!! AATTTGAAATTTAAAATATA+GGG + chr4.4:83396128-83396147 None:intergenic 5.0%
AACAGGCGCAAGGTAGTCAT+AGG + chr4.4:83395796-83395815 None:intergenic 50.0%
AATCAGGAATGTTGAGGGGC+TGG + chr4.4:83396980-83396999 None:intergenic 50.0%
ACAGGCGCAAGGTAGTCATA+GGG + chr4.4:83395795-83395814 None:intergenic 50.0%
AGGCAGGTTGCCTTTGTACT+TGG + chr4.4:83396646-83396665 None:intergenic 50.0%
ATGTCAAGGGAAGGTGGTGT+GGG - chr4.4:83397215-83397234 MS.gene89793:intron 50.0%
CAATTCCTTCAACAGGCGCA+AGG + chr4.4:83395806-83395825 None:intergenic 50.0%
CACAAGTGGCGGCACTAAAA+TGG - chr4.4:83396701-83396720 MS.gene89793:intron 50.0%
CAGAACCTTGGTTGCACTTG+TGG - chr4.4:83395628-83395647 MS.gene89793:CDS 50.0%
CCACCTTCCCTTGACATGTT+CGG + chr4.4:83397212-83397231 None:intergenic 50.0%
CTTGCGCCTGTTGAAGGAAT+TGG - chr4.4:83395804-83395823 MS.gene89793:CDS 50.0%
GAGCCTACACCACAAGCAAA+AGG + chr4.4:83397456-83397475 None:intergenic 50.0%
TCTCCGAACATGTCAAGGGA+AGG - chr4.4:83397206-83397225 MS.gene89793:intron 50.0%
TGGACCCTAATGGCATGGTA+AGG + chr4.4:83396812-83396831 None:intergenic 50.0%
TGGACGTCAAGAATGCTCCA+AGG + chr4.4:83397486-83397505 None:intergenic 50.0%
! CGCACTGTGTTACTCAATCC+TGG - chr4.4:83396927-83396946 MS.gene89793:intron 50.0%
!! GATAAGCATTGGTGAAGCCG+AGG - chr4.4:83397364-83397383 MS.gene89793:CDS 50.0%
!!! AAGTGGGCTGGGAAACCTTT+TGG - chr4.4:83397519-83397538 MS.gene89793:CDS 50.0%
CAGGCGCAAGGTAGTCATAG+GGG + chr4.4:83395794-83395813 None:intergenic 55.0%
CATGTCAAGGGAAGGTGGTG+TGG - chr4.4:83397214-83397233 MS.gene89793:intron 55.0%
CCGAACATGTCAAGGGAAGG+TGG - chr4.4:83397209-83397228 MS.gene89793:intron 55.0%
GACTACCTTGCGCCTGTTGA+AGG - chr4.4:83395798-83395817 MS.gene89793:CDS 55.0%
GTTTCCCAGCCCACTTGTCA+AGG + chr4.4:83397515-83397534 None:intergenic 55.0%
TTAGTGCCGCCACTTGTGCA+AGG + chr4.4:83396699-83396718 None:intergenic 55.0%
TTGGTGAAGCCGAGGTAAGG+TGG - chr4.4:83397372-83397391 MS.gene89793:CDS 55.0%
! GCATTGGTGAAGCCGAGGTA+AGG - chr4.4:83397369-83397388 MS.gene89793:CDS 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG + chr4.4:83396975-83396994 None:intergenic 55.0%
!! GTGTAGGCTCTGAGCATCCT+TGG - chr4.4:83397466-83397485 MS.gene89793:CDS 55.0%
! CCTGGCGCAGCACCACATTT+TGG - chr4.4:83396945-83396964 MS.gene89793:intron 60.0%
!! CCAAAATGTGGTGCTGCGCC+AGG + chr4.4:83396948-83396967 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 83395586 83397561 83395586 ID=MS.gene89793
chr4.4 mRNA 83395586 83397561 83395586 ID=MS.gene89793.t1;Parent=MS.gene89793
chr4.4 exon 83397265 83397561 83397265 ID=MS.gene89793.t1.exon1;Parent=MS.gene89793.t1
chr4.4 CDS 83397265 83397561 83397265 ID=cds.MS.gene89793.t1;Parent=MS.gene89793.t1
chr4.4 exon 83395762 83396574 83395762 ID=MS.gene89793.t1.exon2;Parent=MS.gene89793.t1
chr4.4 CDS 83395762 83396574 83395762 ID=cds.MS.gene89793.t1;Parent=MS.gene89793.t1
chr4.4 exon 83395586 83395675 83395586 ID=MS.gene89793.t1.exon3;Parent=MS.gene89793.t1
chr4.4 CDS 83395586 83395675 83395586 ID=cds.MS.gene89793.t1;Parent=MS.gene89793.t1
Gene Sequence

>MS.gene89793

ATGTATTTTCTGTGCAATTCTTGTTACAAATTCACTACTATTCAGAACCTTGGTTGCACTTGTGGAAAACCAACAAAGAGACCTCGAAATTTGGATTCTGAGGGTCAGGGTAATAGTACTAGTGTGGATGCTCAAAATGAGGTCTTTCTTAGAGAAAATGGTTCAACTTTTTTGGTTTCCGATGATTTGAAGATTGTGCCAAGTTCCCCTATGACTACCTTGCGCCTGTTGAAGGAATTGGGTTATTCAGATTTGACTCAACTAGAGGAAATCACACACAACATTGGCAAGCAAGAGGTAATTATGTTGTGTATCTGTATTTATCACAATTCTCATGCCCCCATAACCTTTTACTTGCATATGATCTTAGACACATCTAAATTTAAATGATGAACATTCACCTCATGCTTTAAAGTTGCGGTTACGTTGTGACCTTTATTTTTGTAAAATAAACAAATGTGACCACAGTTGTGATTATAATATTGCAGTATGCTTCACCCTTTAGGATACATCAGTTAGTGTTACGAACCCGAATTTTTCCCTATATTTTAAATTTCAAATTTGCAACACCAGCGACATTTAATTGATATTTTGAAGGGCATGGTGTTACTTAAAATAAGCAAAAGAAAATATATAAAAAACGATTCCTTGTATTTCAACCCAATGTAAACAAATGACAATAAGAAAGAGTAGAAACATGCTAGGATAATTAGAGTCCAAAGCAAGCAAATGACAATAAAGTAAGAGAAGAAACATGATAGAATAAGCAGAGGAATATATTATGTATATATAATTTGAAGAAAAAGGACCATGTTGCACATCTTATTCATTTGGGAAAAACTTGAAATCCTTGGGTGAATGGTTTCTTCTATTTTCATTCAATAAAATTTCTCCTTTTTCATCTAGTTTTTTTTTATATCAATTTTCCAATTCTTCTCTTGGCTAGTTCTTAATGTTATGAATGATTATTTGCATTTTGATGTGTAGATATTGAACCTACTCAAGTATAGCTTAACCTCTCACGAGCCTATGACAAATACAATTTTGAAAAGTAGCTCCAAGTACAAAGGCAACCTGCCTAACCAATCTTCATCAGCCGTTAGAGTAAAACCTTGCACAAGTGGCGGCACTAAAATGGATGTTAAGGTAGTTCGAAGTATATCTCAGAAAAAGATCATCTTTGTAGAAGCCAACGGTGATTTTGTTTCCTTCATTTTTAGCTTCCTTACCATGCCATTAGGGTCCATTTTAAAGCTTTCAGATGGTAACTCGTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGAACTTGGACTCTTCATTGTGGACTGACTCACGCACTGTGTTACTCAATCCTGGCGCAGCACCACATTTTGGTTGTCCAAACCAGCCCCTCAACATTCCTGATTTAAAACCGCCCACTACATATTATTATGGTACTGGTGTACCAAATTTTGGTTGTCCAAACCAATTCCTCAACATTTCTGATTTAAAACTGCCCACTACATATTATTATGGTACAGGTATACCACATAAATATATCAAGGAAGGAGTGATTTCTAAGACAAAAGAATCCATTTTTAATGCAAAACTCTTGACTGCAGTTGATCCAAAATCTCCGAACATGTCAAGGGAAGGTGGTGTGGGATTTGTGCAGAGATTAGCATTATACGGTGTAGGAGATGATCTAAAAGTAAAATCACTTTCAGCTAATTCTTTCCTTTTATATTTGAAAGAATTAAGCCTTCCCATTGAAGATCTTCAAGTGGAGGTGATAAGCATTGGTGAAGCCGAGGTAAGGTGGTTATTATTTTATCAGTGAATTTATAATTTTTTTCGTTTTTATTTTAACCTTTTTGATTCCTTTTGCTTGTGGTGTAGGCTCTGAGCATCCTTGGAGCATTCTTGACGTCCAAATTTACCTTGACAAGTGGGCTGGGAAACCTTTTGGATCTTCCTAAACAAGAATAA
Protein sequence

>MS.gene89793.t1

MYFLCNSCYKFTTIQNLGCTCGKPTKRPRNLDSEGQGNSTSVDAQNEVFLRENGSTFLVSDDLKIVPSSPMTTLRLLKELGYSDLTQLEEITHNIGKQEILNLLKYSLTSHEPMTNTILKSSSKYKGNLPNQSSSAVRVKPCTSGGTKMDVKVVRSISQKKIIFVEANGDFVSFIFSFLTMPLGSILKLSDGNSFAGCVGNLYKSVENLDSSLWTDSRTVLLNPGAAPHFGCPNQPLNIPDLKPPTTYYYGTGVPNFGCPNQFLNISDLKLPTTYYYGTGIPHKYIKEGVISKTKESIFNAKLLTAVDPKSPNMSREGGVGFVQRLALYGVGDDLKVKSLSANSFLLYLKELSLPIEDLQVEVISIGEAEALSILGAFLTSKFTLTSGLGNLLDLPKQE