AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70037

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70037.t1 MTR_4g017410 69.186 516 119 5 8 514 4 488 0.0 682
MS.gene70037.t1 MTR_4g017440 64.559 522 156 6 4 515 5 507 0.0 631
MS.gene70037.t1 MTR_4g017830 61.804 521 167 8 6 514 5 505 0.0 618
MS.gene70037.t1 MTR_4g017550 66.242 471 130 10 43 506 21 469 0.0 571
MS.gene70037.t1 MTR_4g017610 59.844 513 163 9 1 507 1 476 0.0 566
MS.gene70037.t1 MTR_4g017470 60.038 523 151 8 1 514 1 474 0.0 562
MS.gene70037.t1 MTR_4g019340 59.921 509 140 10 12 499 37 502 0.0 549
MS.gene70037.t1 MTR_4g019310 39.887 529 252 16 1 506 1 486 1.32e-104 328
MS.gene70037.t1 MTR_7g066570 34.070 543 274 19 1 516 1 486 1.51e-65 227
MS.gene70037.t1 MTR_7g066350 32.950 522 249 14 7 506 3 445 1.03e-64 222
MS.gene70037.t1 MTR_7g058710 31.716 536 273 13 1 506 1 473 2.63e-63 219
MS.gene70037.t1 MTR_7g063660 33.140 516 280 16 9 509 5 470 1.46e-58 206
MS.gene70037.t1 MTR_7g066340 36.170 376 204 11 1 357 1 359 4.21e-58 204
MS.gene70037.t1 MTR_7g066340 28.000 250 130 7 268 499 10 227 5.86e-16 81.6
MS.gene70037.t1 MTR_7g066340 26.609 233 130 5 6 238 256 447 2.38e-12 70.1
MS.gene70037.t1 MTR_7g066430 32.000 550 288 16 14 533 5 498 4.91e-58 205
MS.gene70037.t1 MTR_7g066430 26.800 250 144 6 7 238 251 479 3.20e-14 76.3
MS.gene70037.t1 MTR_7g063900 32.061 524 281 14 11 509 8 481 6.59e-57 201
MS.gene70037.t1 MTR_7g066260 31.579 532 252 18 6 506 8 458 1.11e-55 198
MS.gene70037.t1 MTR_7g063880 30.857 525 283 15 1 509 1 461 2.03e-55 197
MS.gene70037.t1 MTR_7g066410 31.618 544 275 17 14 506 11 508 2.41e-55 198
MS.gene70037.t1 MTR_7g062280 31.963 535 274 15 9 509 6 484 1.80e-54 195
MS.gene70037.t1 MTR_7g062300 32.336 535 273 19 1 509 1 472 3.01e-54 194
MS.gene70037.t1 MTR_7g066400 30.769 507 251 16 14 506 13 433 1.01e-52 189
MS.gene70037.t1 MTR_7g012680 30.669 538 259 15 2 515 3 450 3.49e-52 187
MS.gene70037.t1 MTR_1g041315 35.025 394 211 14 9 371 10 389 9.98e-51 184
MS.gene70037.t1 MTR_7g063890 31.440 493 268 14 14 484 28 472 1.84e-50 184
MS.gene70037.t1 MTR_7g066580 29.961 514 267 14 19 511 1 442 8.66e-49 178
MS.gene70037.t1 MTR_7g063840 29.720 535 274 17 6 509 3 466 1.95e-47 175
MS.gene70037.t1 MTR_7g063830 29.623 530 259 15 1 509 1 437 5.77e-47 173
MS.gene70037.t1 MTR_7g066290 36.216 370 200 12 6 355 8 361 3.34e-46 170
MS.gene70037.t1 MTR_7g066360 35.616 365 206 11 2 355 5 351 1.18e-45 170
MS.gene70037.t1 MTR_7g063920 30.228 526 253 17 1 509 1 429 2.61e-45 168
MS.gene70037.t1 MTR_7g058700 33.518 361 153 10 23 347 529 838 5.44e-43 167
MS.gene70037.t1 MTR_7g063820 31.132 530 278 19 9 509 6 477 8.80e-43 162
MS.gene70037.t1 MTR_7g066310 28.125 512 211 14 14 507 8 380 4.51e-42 158
MS.gene70037.t1 MTR_2g029400 28.743 501 239 13 9 498 10 403 4.72e-40 152
MS.gene70037.t1 MTR_7g066480 35.588 340 169 12 14 333 17 326 5.14e-40 153
MS.gene70037.t1 MTR_3g007740 27.379 515 282 14 9 509 5 441 6.53e-39 150
MS.gene70037.t1 MTR_7g063670 28.320 512 282 16 10 509 12 450 1.71e-38 149
MS.gene70037.t1 MTR_4g019240 58.400 125 48 2 1 125 1 121 1.81e-38 141
MS.gene70037.t1 MTR_3g065200 26.962 497 274 12 14 505 4 416 2.37e-38 149
MS.gene70037.t1 MTR_7g066490 31.024 332 179 9 14 332 17 311 5.03e-31 126
MS.gene70037.t1 MTR_7g066280 31.799 239 126 7 102 331 1 211 2.69e-24 106
MS.gene70037.t1 MTR_7g066280 24.615 260 151 7 6 238 143 384 5.58e-12 68.9
MS.gene70037.t1 MTR_1g014060 32.589 224 125 7 22 236 9 215 2.95e-23 103
MS.gene70037.t1 MTR_7g066680 29.643 280 158 7 221 490 37 287 1.56e-20 94.0
MS.gene70037.t1 MTR_7g063720 29.675 246 118 5 273 509 26 225 4.67e-20 90.5
MS.gene70037.t1 MTR_7g062270 34.184 196 103 7 8 194 5 183 4.78e-20 93.2
MS.gene70037.t1 MTR_8g445860 27.881 269 113 9 269 504 12 232 1.60e-17 83.2
MS.gene70037.t1 MTR_8g445860 28.512 242 145 7 11 241 11 235 3.55e-14 73.2
MS.gene70037.t1 MTR_1g115440 29.752 242 139 8 14 241 12 236 9.00e-17 80.9
MS.gene70037.t1 MTR_1g115440 25.794 252 143 6 269 504 10 233 7.35e-15 75.1
MS.gene70037.t1 MTR_7g100420 28.032 371 201 14 152 506 73 393 3.25e-16 82.0
MS.gene70037.t1 MTR_7g100420 58.000 50 21 0 13 62 39 88 2.55e-11 66.6
MS.gene70037.t1 MTR_0150s0010 29.661 236 139 6 14 237 11 231 6.20e-16 78.2
MS.gene70037.t1 MTR_0150s0010 28.287 251 146 7 265 504 5 232 2.51e-14 73.6
MS.gene70037.t1 MTR_8g023240 22.376 362 229 12 13 352 37 368 3.56e-15 79.7
MS.gene70037.t1 MTR_6g006800 72.917 48 11 1 1 48 1 46 6.43e-15 70.5
MS.gene70037.t1 MTR_1g115450 42.500 80 38 1 269 340 6 85 7.12e-14 69.7
MS.gene70037.t1 MTR_1g115455 39.080 87 45 1 262 340 1 87 2.32e-13 70.9
MS.gene70037.t1 MTR_1g115455 26.271 236 151 5 14 241 10 230 8.56e-12 66.2
MS.gene70037.t1 MTR_8g445920 41.791 67 39 0 265 331 5 71 9.79e-13 65.5
MS.gene70037.t1 MTR_8g044890 41.791 67 39 0 265 331 5 71 9.79e-13 65.5
MS.gene70037.t1 MTR_0150s0030 38.372 86 43 2 269 345 7 91 2.95e-12 67.0
MS.gene70037.t1 MTR_8g445910 39.535 86 42 2 269 345 7 91 1.16e-11 66.2
MS.gene70037.t1 MTR_1g115470 41.250 80 39 1 269 340 5 84 1.39e-11 67.8
MS.gene70037.t1 MTR_8g044880 39.506 81 40 1 269 340 7 87 2.11e-11 65.9
MS.gene70037.t1 MTR_4g019330 47.500 80 40 2 437 514 107 186 8.87e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70037.t1 AT5G01130 30.934 514 271 16 7 509 3 443 8.02e-56 197
MS.gene70037.t1 AT5G01120 31.169 539 306 19 10 525 6 502 2.06e-55 198
MS.gene70037.t1 AT5G01140 30.798 526 262 19 16 517 12 459 1.26e-51 186
MS.gene70037.t1 AT5G01150 30.874 515 287 16 16 509 12 478 4.40e-51 186
MS.gene70037.t1 AT5G43240 31.120 527 284 18 13 506 9 489 6.81e-50 182
MS.gene70037.t1 AT5G43240 31.120 527 284 18 13 506 9 489 6.81e-50 182
MS.gene70037.t1 AT5G43240 31.120 527 284 18 13 506 9 489 6.81e-50 182
MS.gene70037.t1 AT3G09140 29.282 543 285 18 10 509 6 492 1.21e-49 182
MS.gene70037.t1 AT3G09110 35.843 332 184 11 10 331 6 318 4.40e-46 167
MS.gene70037.t1 AT3G09140 27.846 571 285 19 10 509 6 520 1.25e-44 168
MS.gene70037.t1 AT3G09120 33.828 337 175 12 10 324 3 313 3.49e-41 153
MS.gene70037.t1 AT3G09120 27.016 248 144 6 270 504 6 229 1.83e-15 78.6
MS.gene70037.t1 AT5G37320 28.350 515 290 17 20 509 18 478 3.19e-38 149
MS.gene70037.t1 AT5G43240 29.107 347 193 10 179 506 33 345 5.69e-24 104

Find 176 sgRNAs with CRISPR-Local

Find 246 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAGAAACTTTGGTCCATTT+TGG 0.096796 4.2:-84441425 None:intergenic
AAATCAACAGCCTCGAAATT+TGG 0.164742 4.2:+84441365 MS.gene70037:CDS
GCCACCTTTGGCTCGCTATT+TGG 0.177964 4.2:+84443401 MS.gene70037:CDS
GCCTCTCACAAATACAATTT+TGG 0.184722 4.2:+84442225 MS.gene70037:CDS
GGACCTCCTTCCGTCAATTT+TGG 0.186758 4.2:+84443332 MS.gene70037:CDS
CCTTGGTTCCAGTGCAATTA+AGG 0.221659 4.2:-84442782 None:intergenic
AGGATCCATTGTAAAGCTTT+TGG 0.229184 4.2:+84442438 MS.gene70037:CDS
CTTGCAGCTGTTGATGGAAT+TGG 0.234153 4.2:+84441491 MS.gene70037:CDS
TTCTTTACTCGGCGTGATAT+CGG 0.236275 4.2:+84443361 MS.gene70037:CDS
GAACACCCAGCTTATTATTA+TGG 0.239352 4.2:+84442598 MS.gene70037:CDS
CCAAAATGGACCAAAGTTTC+TGG 0.240644 4.2:+84441425 MS.gene70037:CDS
TGTTGACCCCTCTTTCTCTT+TGG 0.261951 4.2:-84442631 None:intergenic
GCAGCTTCTATACCGTCTTT+TGG 0.269844 4.2:+84443623 MS.gene70037:CDS
ACTACTCCTACGAATTCTTT+TGG 0.270872 4.2:+84443437 MS.gene70037:CDS
TCATCATCCACCTCTGCATT+TGG 0.274399 4.2:+84443233 MS.gene70037:CDS
TTGATCATTGTTGCTGTTGA+TGG 0.292170 4.2:-84441076 None:intergenic
GCAAAGTAAATCTCAGAAAA+AGG 0.300596 4.2:+84442351 MS.gene70037:CDS
TCTTCTGGTACACCGTCTTT+TGG 0.322220 4.2:+84443593 MS.gene70037:CDS
TCGGCTTCAAATACAGCTTC+AGG 0.323250 4.2:+84443467 MS.gene70037:CDS
GACTTACTTGCAGCTGTTGA+TGG 0.339065 4.2:+84441485 MS.gene70037:CDS
CCGTTTGGAGTACCGTCCTT+TGG 0.341591 4.2:+84443524 MS.gene70037:CDS
GCTTTCCACAAGTGCAATAA+AGG 0.345430 4.2:-84441338 None:intergenic
GGATTGAGTAACACAGAAAC+AGG 0.345671 4.2:-84442520 None:intergenic
TCAGATTTCGCGGATTATAA+TGG 0.349431 4.2:+84442658 MS.gene70037:CDS
AAGGCTTCTACACCATTGTT+TGG 0.357666 4.2:+84443683 MS.gene70037:CDS
ACCTACACTCAGACGATGTT+TGG 0.357820 4.2:+84441166 MS.gene70037:CDS
TTGCGCCGTCGTTTGGAGTT+TGG 0.360350 4.2:+84443720 MS.gene70037:CDS
GGAGTACCATCATCATCTTC+TGG 0.362322 4.2:+84443578 MS.gene70037:CDS
AGTTTCCTTACCATGCCATT+AGG 0.365207 4.2:+84442418 MS.gene70037:CDS
TTGCAGCTGTTGATGGAATT+GGG 0.365599 4.2:+84441492 MS.gene70037:CDS
TCGGCTTCTACACCATTGTT+TGG 0.369254 4.2:+84443557 MS.gene70037:CDS
GTGATAGAAGAAAAGATAGA+AGG 0.378763 4.2:+84442715 MS.gene70037:CDS
CACACACAACATTGGAAAAC+AGG 0.378988 4.2:+84441545 MS.gene70037:CDS
TTTCGAGGCTGTTGATTTGT+TGG 0.384093 4.2:-84441360 None:intergenic
ACGGCTTCTACACCGTCGTT+TGG 0.385815 4.2:+84443653 MS.gene70037:CDS
TGCTGTTGATGGTGGCTAAA+AGG 0.392005 4.2:-84441065 None:intergenic
GTGTAGGCTTTGAACTTCCT+TGG 0.395429 4.2:+84443065 MS.gene70037:intron
TGTATTTGTGAGAGGCTCAT+TGG 0.399502 4.2:-84442218 None:intergenic
AATAGTGCGGAAGCCCAAAA+TGG 0.402429 4.2:+84441411 MS.gene70037:CDS
ACAGCTTCAGGGTCGTCGTT+TGG 0.405842 4.2:+84443479 MS.gene70037:CDS
CAGTGATTCACATGGACTCC+TGG 0.416519 4.2:-84441223 None:intergenic
GTTTCTGTGTTACTCAATCC+TGG 0.427223 4.2:+84442523 MS.gene70037:CDS
TTTGAATGTTCGATTGCAAA+AGG 0.429014 4.2:-84443162 None:intergenic
TGCGAAGTCCTTAATTGCAC+TGG 0.430913 4.2:+84442774 MS.gene70037:CDS
AAAGAATTCGTAGGAGTAGT+AGG 0.438431 4.2:-84443434 None:intergenic
CCGGCATCTTCAACTCCGTT+TGG 0.439075 4.2:+84443509 MS.gene70037:CDS
CCTGGTATAGCACCACAGTT+TGG 0.441678 4.2:+84442541 MS.gene70037:CDS
AGGAGAAGAAGTTGCGGCAG+AGG 0.444556 4.2:-84443270 None:intergenic
CAAAACCTTTATTGCACTTG+TGG 0.445323 4.2:+84441333 MS.gene70037:CDS
GAGAGGCTCATTGGAGGTTA+AGG 0.452511 4.2:-84442209 None:intergenic
CAAAGGTGGCGGAACCGAAA+AGG 0.455116 4.2:-84443388 None:intergenic
CATAAGTAAAATCAATGTTA+AGG 0.456872 4.2:+84442324 MS.gene70037:CDS
CCTGTACCATAATAATAAGC+TGG 0.458318 4.2:-84442604 None:intergenic
AACGACGGCGCAAAAGCCGC+TGG 0.458595 4.2:-84443710 None:intergenic
TTTGACCTGTTGGGAGACCT+TGG 0.460627 4.2:-84442799 None:intergenic
AGTGTAGGTCTTGGACGGTT+TGG 0.461547 4.2:-84441152 None:intergenic
ACTGTTGATGCAGATTGCTT+AGG 0.463635 4.2:-84442277 None:intergenic
CCCTCCGAATCCAAATTTCG+AGG 0.464453 4.2:-84441375 None:intergenic
TTCAGACGCGACTCAATTAG+AGG 0.464556 4.2:+84441518 MS.gene70037:CDS
TCATCAGACACCAGAAACTT+TGG 0.465061 4.2:-84441435 None:intergenic
TCCTCAATTTCAAAACCAAA+AGG 0.467135 4.2:+84443190 MS.gene70037:CDS
AAAGGTGGCGGAACCGAAAA+GGG 0.469822 4.2:-84443387 None:intergenic
TTTGGATCCTCTTCTTTACT+CGG 0.473778 4.2:+84443350 MS.gene70037:CDS
GGAAGGAGATGGCAAGAAAG+AGG 0.475700 4.2:-84443300 None:intergenic
GGTCTTGGACGGTTTGGTAG+AGG 0.485592 4.2:-84441146 None:intergenic
CCAGCTTATTATTATGGTAC+AGG 0.485999 4.2:+84442604 MS.gene70037:CDS
GGAATGTTGAGTGGTTGGTT+TGG 0.486563 4.2:-84442568 None:intergenic
TGGTGCCAAGTGGAAGAGAT+AGG 0.487529 4.2:-84441035 None:intergenic
TAATGGTAATCGTATAATGA+TGG 0.489399 4.2:+84442675 MS.gene70037:CDS
ACAGCCTCGAAATTTGGATT+CGG 0.490480 4.2:+84441371 MS.gene70037:CDS
TGGGATTTGTGAAGAGAGAA+GGG 0.491787 4.2:+84442833 MS.gene70037:CDS
AGCTTTACAATGGATCCTAA+TGG 0.496722 4.2:-84442433 None:intergenic
CATCGTCTGAGTGTAGGTCT+TGG 0.496962 4.2:-84441161 None:intergenic
TGGAACCAAGGTCTCCCAAC+AGG 0.497799 4.2:+84442794 MS.gene70037:CDS
ACCTTTGGCTCGCTATTTGG+TGG 0.499467 4.2:+84443404 MS.gene70037:CDS
CCAAACTGTGGTGCTATACC+AGG 0.500141 4.2:-84442541 None:intergenic
ATTAACCTTGACTAGTGGAC+TGG 0.503661 4.2:+84443106 MS.gene70037:CDS
AGCTTCCTATCTCTTCCACT+TGG 0.503728 4.2:+84441030 MS.gene70037:CDS
TTGAATGTTCGATTGCAAAA+GGG 0.510409 4.2:-84443161 None:intergenic
GAGAGTGTCAACAAAATCCT+TGG 0.515103 4.2:-84441004 None:intergenic
GCCAAAATTGTATTTGTGAG+AGG 0.523537 4.2:-84442226 None:intergenic
GCCTCGAAATTTGGATTCGG+AGG 0.523747 4.2:+84441374 MS.gene70037:CDS
CCAAAGGACGGTACTCCAAA+CGG 0.525259 4.2:-84443524 None:intergenic
AGAGAAGGGTTATATTGTGT+AGG 0.527009 4.2:+84442847 MS.gene70037:CDS
CGGCTTCAAATACAGCTTCA+GGG 0.527253 4.2:+84443468 MS.gene70037:CDS
GTCGTCGTTTGGAGCATCAC+CGG 0.531624 4.2:+84443490 MS.gene70037:CDS
TGAATGTTCGATTGCAAAAG+GGG 0.532394 4.2:-84443160 None:intergenic
CCATCTCCTTCCTCTTCTAG+AGG 0.534956 4.2:+84443311 MS.gene70037:CDS
CCTTAATTGCACTGGAACCA+AGG 0.535146 4.2:+84442782 MS.gene70037:CDS
AACAAATGTTGCTGTATCCC+AGG 0.536182 4.2:+84441205 MS.gene70037:CDS
ACGACGGCGCAAAAGCCGCT+GGG 0.536665 4.2:-84443709 None:intergenic
GTAACACAGAAACAGGGTTA+AGG 0.537772 4.2:-84442513 None:intergenic
ATCATTGTTGCTGTTGATGG+TGG 0.539275 4.2:-84441073 None:intergenic
CAGGAACACCAAAGAGAAAG+AGG 0.539842 4.2:+84442623 MS.gene70037:CDS
AGTGATTCACATGGACTCCT+GGG 0.542069 4.2:-84441222 None:intergenic
TCTGAGGAATGTTGAGTGGT+TGG 0.545273 4.2:-84442573 None:intergenic
AGCCGATGACGATGCTCCAA+AGG 0.545629 4.2:-84443540 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.547627 4.2:+84442486 MS.gene70037:CDS
AAGGAATAGTGATGATTCTG+AGG 0.547834 4.2:-84441109 None:intergenic
CTGTACCATAATAATAAGCT+GGG 0.548563 4.2:-84442603 None:intergenic
GTCTGAGTGTAGGTCTTGGA+CGG 0.550379 4.2:-84441157 None:intergenic
TGGATCCTAATGGCATGGTA+AGG 0.551509 4.2:-84442423 None:intergenic
TCAACATTCCTCAGATTTCG+CGG 0.551509 4.2:+84442648 MS.gene70037:CDS
ATAGAAGAAAAGATAGAAGG+AGG 0.551511 4.2:+84442718 MS.gene70037:CDS
GTCCTTTGGAGCATCGTCAT+CGG 0.554103 4.2:+84443538 MS.gene70037:CDS
AATGCAGAGGTGGATGATGA+AGG 0.554362 4.2:-84443230 None:intergenic
TTACTTATGTTACCGGTGCA+AGG 0.557753 4.2:-84442310 None:intergenic
TTTCAAGATCATCAAGAGGA+AGG 0.560065 4.2:-84442933 None:intergenic
AAATAGCGAGCCAAAGGTGG+CGG 0.560413 4.2:-84443399 None:intergenic
GTGGGATTTGTGAAGAGAGA+AGG 0.565305 4.2:+84442832 MS.gene70037:CDS
GCCGTTAGAGCTCCAAAAGA+CGG 0.565551 4.2:-84443635 None:intergenic
TTCATTTCAAGATCATCAAG+AGG 0.577676 4.2:-84442937 None:intergenic
AGAAGAGGATCCAAAATTGA+CGG 0.578525 4.2:-84443342 None:intergenic
TCAAAATTAACCTTGACTAG+TGG 0.580631 4.2:+84443101 MS.gene70037:CDS
TACAATGGATCCTAATGGCA+TGG 0.582946 4.2:-84442428 None:intergenic
GAGGAAGTCACACACAACAT+TGG 0.582955 4.2:+84441537 MS.gene70037:CDS
GATAAGCATTGGTGAAGTTG+AGG 0.584497 4.2:+84442963 MS.gene70037:CDS
AGATCCCAAAAGCTTTACAA+TGG 0.585059 4.2:-84442443 None:intergenic
ACCAAATAGCGAGCCAAAGG+TGG 0.585195 4.2:-84443402 None:intergenic
GGCAAGAAAGAGGAGGCAGA+AGG 0.585799 4.2:-84443290 None:intergenic
ATTTGTGAGAGGCTCATTGG+AGG 0.588293 4.2:-84442215 None:intergenic
GGCAGAAGGAGAAGAAGTTG+CGG 0.588454 4.2:-84443276 None:intergenic
GATCCAAAATTGACGGAAGG+AGG 0.589093 4.2:-84443335 None:intergenic
ACCACCAAATAGCGAGCCAA+AGG 0.596309 4.2:-84443405 None:intergenic
GTCTCCCAGTCCACTAGTCA+AGG 0.597674 4.2:-84443111 None:intergenic
CCTCGAAATTTGGATTCGGA+GGG 0.597982 4.2:+84441375 MS.gene70037:CDS
GCCTTTGATGTTCCAAACGA+CGG 0.598640 4.2:-84443665 None:intergenic
AGGAATAGTGATGATTCTGA+GGG 0.601982 4.2:-84441108 None:intergenic
ATTATAATCCGCGAAATCTG+AGG 0.603102 4.2:-84442656 None:intergenic
CCTCTAGAAGAGGAAGGAGA+TGG 0.603237 4.2:-84443311 None:intergenic
CGACGGCGCAAAAGCCGCTG+GGG 0.604534 4.2:-84443708 None:intergenic
GCTGCCGGTGCTCCAAAAGA+CGG 0.605110 4.2:-84443605 None:intergenic
TTGATGCAGATTGCTTAGGT+AGG 0.607887 4.2:-84442273 None:intergenic
GAAGTCAACAAAATCACCGT+TGG 0.609986 4.2:-84442389 None:intergenic
CCAAACGGAGTTGAAGATGC+CGG 0.610712 4.2:-84443509 None:intergenic
GTTAGATTAATGCCTTGCAC+CGG 0.610804 4.2:+84442298 MS.gene70037:CDS
GGAAGGAGGTCCTCTAGAAG+AGG 0.613771 4.2:-84443321 None:intergenic
GGAGGTCCTCTAGAAGAGGA+AGG 0.615174 4.2:-84443317 None:intergenic
CGTATAATGATGGATTGTAG+TGG 0.615640 4.2:+84442685 MS.gene70037:CDS
TGCAGCTGTTGATGGAATTG+GGG 0.616569 4.2:+84441493 MS.gene70037:CDS
ACCGTCGTTTGGAACATCAA+AGG 0.616749 4.2:+84443664 MS.gene70037:CDS
TAAGCTGGGTGTTCAATCTG+AGG 0.617424 4.2:-84442589 None:intergenic
TTAACCTTGACTAGTGGACT+GGG 0.619919 4.2:+84443107 MS.gene70037:CDS
GCAGAGGTGGATGATGAAGG+AGG 0.620324 4.2:-84443227 None:intergenic
GAAATGAAGCTGATAAGCAT+TGG 0.620330 4.2:+84442952 MS.gene70037:CDS
AGGAACACCAAAGAGAAAGA+GGG 0.620352 4.2:+84442624 MS.gene70037:CDS
CAGAGGTGGATGATGAAGGA+GGG 0.620708 4.2:-84443226 None:intergenic
TGCGGCAGAGGTTGTCTGTG+AGG 0.627436 4.2:-84443258 None:intergenic
GAGGATCCAAAATTGACGGA+AGG 0.631203 4.2:-84443338 None:intergenic
AAAGACGGTATAGAAGCTGC+CGG 0.631499 4.2:-84443620 None:intergenic
GTGATATATGCAGAAGCCAA+CGG 0.632346 4.2:+84442373 MS.gene70037:CDS
AAGTAAGTCACAGACGAACA+TGG 0.633871 4.2:-84441471 None:intergenic
TATCACGCCGAGTAAAGAAG+AGG 0.639003 4.2:-84443357 None:intergenic
AGGAGATGGCAAGAAAGAGG+AGG 0.640462 4.2:-84443297 None:intergenic
TGAGGATCCAAATGCAGAGG+TGG 0.643982 4.2:-84443240 None:intergenic
TCAAGAGAATAGTAATAGTG+CGG 0.645716 4.2:+84441398 MS.gene70037:CDS
TTGATGTCAAGAATGCTCCA+AGG 0.651294 4.2:-84443082 None:intergenic
GCCGCTGGGGATCCAAACAA+TGG 0.651558 4.2:-84443695 None:intergenic
GGTGTACCAGAAGATGATGA+TGG 0.659132 4.2:-84443584 None:intergenic
GTTGCCCCAAAAGAATTCGT+AGG 0.667496 4.2:-84443443 None:intergenic
GGTGGCTAAAAGGCGGACAA+TGG 0.668124 4.2:-84441055 None:intergenic
TCCAAACATCGTCTGAGTGT+AGG 0.668557 4.2:-84441167 None:intergenic
ACCATTGTTTGGATCCCCAG+CGG 0.670336 4.2:+84443694 MS.gene70037:CDS
TCAATCTGAGGAATGTTGAG+TGG 0.670562 4.2:-84442577 None:intergenic
GGAACACCAAAGAGAAAGAG+GGG 0.676448 4.2:+84442625 MS.gene70037:CDS
GATGACGATGCTCCAAAGGA+CGG 0.678623 4.2:-84443536 None:intergenic
AGGCGGACAATGGTGCCAAG+TGG 0.686421 4.2:-84441045 None:intergenic
ACACAACATTGGAAAACAGG+AGG 0.691626 4.2:+84441548 MS.gene70037:CDS
CAAAACCAAACTCCAAACGA+CGG 0.692161 4.2:-84443725 None:intergenic
TAATTTGTACAAGAGTGTGG+AGG 0.710790 4.2:+84442489 MS.gene70037:CDS
CTGTGAGGATCCAAATGCAG+AGG 0.714952 4.2:-84443243 None:intergenic
TTCATGCACAGTGATTCACA+TGG 0.718168 4.2:-84441231 None:intergenic
GATGATGGTACTCCAAACAA+TGG 0.720142 4.2:-84443569 None:intergenic
TGGTTTGGACAACCAAACTG+TGG 0.720651 4.2:-84442553 None:intergenic
GATTGAGTAACACAGAAACA+GGG 0.732239 4.2:-84442519 None:intergenic
TGTTGATGGTGGCTAAAAGG+CGG 0.769976 4.2:-84441062 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TCTAAATTTTACCATAATAA+AGG - chr4.2:84441730-84441749 None:intergenic 15.0%
!!! TTTAAGATCTATTCAATTTT+CGG - chr4.2:84441898-84441917 None:intergenic 15.0%
!! CATAAGTAAAATCAATGTTA+AGG + chr4.2:84442324-84442343 MS.gene70037:CDS 20.0%
!! TAATTAATATTCACAATCTC+AGG + chr4.2:84441978-84441997 MS.gene70037:intron 20.0%
!! TTTATTGAGAACCTTTATTA+TGG + chr4.2:84441716-84441735 MS.gene70037:intron 20.0%
!!! ATTGATTTTACTTATGTTAC+CGG - chr4.2:84442320-84442339 None:intergenic 20.0%
!!! TCACAAATATATGATTTTAG+AGG - chr4.2:84441794-84441813 None:intergenic 20.0%
!!! TTAAGATCTATTCAATTTTC+GGG - chr4.2:84441897-84441916 None:intergenic 20.0%
!!! TTTGTCTACTATGATTTTAA+GGG - chr4.2:84440953-84440972 None:intergenic 20.0%
! AACCACAAATTAAAACAATG+AGG - chr4.2:84441693-84441712 None:intergenic 25.0%
! TAATGGTAATCGTATAATGA+TGG + chr4.2:84442675-84442694 MS.gene70037:CDS 25.0%
! TCAATATCTGTACATCAAAA+AGG - chr4.2:84442174-84442193 None:intergenic 25.0%
! TGAAGATTCATGATTAAGAT+TGG - chr4.2:84441670-84441689 None:intergenic 25.0%
!! CACACAAAAAACTTGTTTAA+TGG - chr4.2:84441285-84441304 None:intergenic 25.0%
!! GTTTGTCTACTATGATTTTA+AGG - chr4.2:84440954-84440973 None:intergenic 25.0%
!! TTGTCTACTATGATTTTAAG+GGG - chr4.2:84440952-84440971 None:intergenic 25.0%
!!! AAGCAACAAAGTTATTTTTC+TGG + chr4.2:84440975-84440994 MS.gene70037:CDS 25.0%
!!! ACCTTTTTCGAACAAAAAAA+AGG - chr4.2:84442140-84442159 None:intergenic 25.0%
!!! TCCTTTTTTTTGTTCGAAAA+AGG + chr4.2:84442136-84442155 MS.gene70037:intron 25.0%
ACAAGTAAAGGAAATCAAAG+AGG - chr4.2:84443044-84443063 None:intergenic 30.0%
AGGTAATTAATTCTGTTGTG+TGG + chr4.2:84441568-84441587 MS.gene70037:intron 30.0%
ATAGAAGAAAAGATAGAAGG+AGG + chr4.2:84442718-84442737 MS.gene70037:CDS 30.0%
CTGTACCATAATAATAAGCT+GGG - chr4.2:84442606-84442625 None:intergenic 30.0%
GCAAAGTAAATCTCAGAAAA+AGG + chr4.2:84442351-84442370 MS.gene70037:CDS 30.0%
GGAAGTCAATTAGTTAGATA+AGG + chr4.2:84442061-84442080 MS.gene70037:intron 30.0%
GTGATAGAAGAAAAGATAGA+AGG + chr4.2:84442715-84442734 MS.gene70037:CDS 30.0%
TCAAAATTAACCTTGACTAG+TGG + chr4.2:84443101-84443120 MS.gene70037:CDS 30.0%
TCAAGAGAATAGTAATAGTG+CGG + chr4.2:84441398-84441417 MS.gene70037:CDS 30.0%
TCCTCAATTTCAAAACCAAA+AGG + chr4.2:84443190-84443209 MS.gene70037:CDS 30.0%
TTCATTTCAAGATCATCAAG+AGG - chr4.2:84442940-84442959 None:intergenic 30.0%
TTGAATGTTCGATTGCAAAA+GGG - chr4.2:84443164-84443183 None:intergenic 30.0%
TTGTACCAAGTTTAAAGGTT+CGG + chr4.2:84441942-84441961 MS.gene70037:intron 30.0%
TTTGAATGTTCGATTGCAAA+AGG - chr4.2:84443165-84443184 None:intergenic 30.0%
! AGAGGTTTTTGATGTTTTCA+AGG - chr4.2:84441131-84441150 None:intergenic 30.0%
! TCTTTGATTTCCTTTACTTG+TGG + chr4.2:84443043-84443062 MS.gene70037:intron 30.0%
!! TACTATGATTTTAAGGGGAA+TGG - chr4.2:84440947-84440966 None:intergenic 30.0%
!!! CACCTCATTGTTTTAATTTG+TGG + chr4.2:84441688-84441707 MS.gene70037:intron 30.0%
!!! TCCTTTTGGTTTTGAAATTG+AGG - chr4.2:84443194-84443213 None:intergenic 30.0%
!!! TTGGTTTTGAAATTGAGGAA+GGG - chr4.2:84443189-84443208 None:intergenic 30.0%
!!! TTTGGTTTTGAAATTGAGGA+AGG - chr4.2:84443190-84443209 None:intergenic 30.0%
AAAGAATTCGTAGGAGTAGT+AGG - chr4.2:84443437-84443456 None:intergenic 35.0%
AAATCAACAGCCTCGAAATT+TGG + chr4.2:84441365-84441384 MS.gene70037:CDS 35.0%
AAGAGTAGAATAGTGCAGTT+AGG + chr4.2:84442039-84442058 MS.gene70037:intron 35.0%
AAGGAATAGTGATGATTCTG+AGG - chr4.2:84441112-84441131 None:intergenic 35.0%
AGAAGAGGATCCAAAATTGA+CGG - chr4.2:84443345-84443364 None:intergenic 35.0%
AGAGAAGGGTTATATTGTGT+AGG + chr4.2:84442847-84442866 MS.gene70037:CDS 35.0%
AGAGTAGAATAGTGCAGTTA+GGG + chr4.2:84442040-84442059 MS.gene70037:intron 35.0%
AGATCCCAAAAGCTTTACAA+TGG - chr4.2:84442446-84442465 None:intergenic 35.0%
AGATGCGAATGAACAAAAAG+TGG - chr4.2:84442892-84442911 None:intergenic 35.0%
AGCTTTACAATGGATCCTAA+TGG - chr4.2:84442436-84442455 None:intergenic 35.0%
AGGAATAGTGATGATTCTGA+GGG - chr4.2:84441111-84441130 None:intergenic 35.0%
AGTACTACTACATTTCCTGA+AGG - chr4.2:84441839-84441858 None:intergenic 35.0%
ATTATAATCCGCGAAATCTG+AGG - chr4.2:84442659-84442678 None:intergenic 35.0%
ATTTCCTTTACTTGTGGTGT+AGG + chr4.2:84443049-84443068 MS.gene70037:intron 35.0%
CAAAACCTTTATTGCACTTG+TGG + chr4.2:84441333-84441352 MS.gene70037:CDS 35.0%
CAGGAAATGTAGTAGTACTA+GGG + chr4.2:84441840-84441859 MS.gene70037:intron 35.0%
CCAGCTTATTATTATGGTAC+AGG + chr4.2:84442604-84442623 MS.gene70037:CDS 35.0%
CCTGTACCATAATAATAAGC+TGG - chr4.2:84442607-84442626 None:intergenic 35.0%
GAAATGAAGCTGATAAGCAT+TGG + chr4.2:84442952-84442971 MS.gene70037:CDS 35.0%
GAACACCCAGCTTATTATTA+TGG + chr4.2:84442598-84442617 MS.gene70037:CDS 35.0%
GATTGAGTAACACAGAAACA+GGG - chr4.2:84442522-84442541 None:intergenic 35.0%
GCCAAAATTGTATTTGTGAG+AGG - chr4.2:84442229-84442248 None:intergenic 35.0%
GTACTACTACATTTCCTGAA+GGG - chr4.2:84441838-84441857 None:intergenic 35.0%
TCAGGAAATGTAGTAGTACT+AGG + chr4.2:84441839-84441858 MS.gene70037:intron 35.0%
TGAATGTTCGATTGCAAAAG+GGG - chr4.2:84443163-84443182 None:intergenic 35.0%
TTTCAAGATCATCAAGAGGA+AGG - chr4.2:84442936-84442955 None:intergenic 35.0%
TTTGGATCCTCTTCTTTACT+CGG + chr4.2:84443350-84443369 MS.gene70037:CDS 35.0%
! ACTACTCCTACGAATTCTTT+TGG + chr4.2:84443437-84443456 MS.gene70037:CDS 35.0%
! AGATTTTAGGCAGTTTCGAA+AGG - chr4.2:84443136-84443155 None:intergenic 35.0%
! AGGATCCATTGTAAAGCTTT+TGG + chr4.2:84442438-84442457 MS.gene70037:CDS 35.0%
! AGGTCAAAAATAGGTGTTGT+GGG + chr4.2:84442814-84442833 MS.gene70037:CDS 35.0%
! CAGAAACTTTGGTCCATTTT+GGG - chr4.2:84441427-84441446 None:intergenic 35.0%
! CTACTCCTACGAATTCTTTT+GGG + chr4.2:84443438-84443457 MS.gene70037:CDS 35.0%
! GCCTCTCACAAATACAATTT+TGG + chr4.2:84442225-84442244 MS.gene70037:CDS 35.0%
! GGATCCATTGTAAAGCTTTT+GGG + chr4.2:84442439-84442458 MS.gene70037:CDS 35.0%
! GTAGCTTGTACCAAGTTTAA+AGG + chr4.2:84441937-84441956 MS.gene70037:intron 35.0%
! TAATTTGTACAAGAGTGTGG+AGG + chr4.2:84442489-84442508 MS.gene70037:CDS 35.0%
! TACTCCTACGAATTCTTTTG+GGG + chr4.2:84443439-84443458 MS.gene70037:CDS 35.0%
! TCAGATTTCGCGGATTATAA+TGG + chr4.2:84442658-84442677 MS.gene70037:CDS 35.0%
! TGGTAATTTGTACAAGAGTG+TGG + chr4.2:84442486-84442505 MS.gene70037:CDS 35.0%
! TTGATCATTGTTGCTGTTGA+TGG - chr4.2:84441079-84441098 None:intergenic 35.0%
!! CAAAAGGGGTTTGAGATTTT+AGG - chr4.2:84443149-84443168 None:intergenic 35.0%
!! CGTATAATGATGGATTGTAG+TGG + chr4.2:84442685-84442704 MS.gene70037:CDS 35.0%
!! GATTTTAGAGGTTTCCACAA+TGG - chr4.2:84441782-84441801 None:intergenic 35.0%
!! TATTTTTCTGGAAGCAACCA+AGG + chr4.2:84440987-84441006 MS.gene70037:CDS 35.0%
!!! AACACCTATTTTTGACCTGT+TGG - chr4.2:84442812-84442831 None:intergenic 35.0%
!!! ACACCTATTTTTGACCTGTT+GGG - chr4.2:84442811-84442830 None:intergenic 35.0%
!!! AGGTAGGCTGTCTTTATTTT+TGG - chr4.2:84442260-84442279 None:intergenic 35.0%
!!! GGTGTGAAGTTTTTTCCTTT+TGG - chr4.2:84443208-84443227 None:intergenic 35.0%
AAAGCCTACACCACAAGTAA+AGG - chr4.2:84443056-84443075 None:intergenic 40.0%
AACAAATGTTGCTGTATCCC+AGG + chr4.2:84441205-84441224 MS.gene70037:CDS 40.0%
AAGGCTTCTACACCATTGTT+TGG + chr4.2:84443683-84443702 MS.gene70037:CDS 40.0%
AAGTAAGTCACAGACGAACA+TGG - chr4.2:84441474-84441493 None:intergenic 40.0%
ACACAACATTGGAAAACAGG+AGG + chr4.2:84441548-84441567 MS.gene70037:CDS 40.0%
ACAGCCTCGAAATTTGGATT+CGG + chr4.2:84441371-84441390 MS.gene70037:CDS 40.0%
ACTGTTGATGCAGATTGCTT+AGG - chr4.2:84442280-84442299 None:intergenic 40.0%
AGGAACACCAAAGAGAAAGA+GGG + chr4.2:84442624-84442643 MS.gene70037:CDS 40.0%
AGTTTCCTTACCATGCCATT+AGG + chr4.2:84442418-84442437 MS.gene70037:CDS 40.0%
CAAAACCAAACTCCAAACGA+CGG - chr4.2:84443728-84443747 None:intergenic 40.0%
CACACACAACATTGGAAAAC+AGG + chr4.2:84441545-84441564 MS.gene70037:CDS 40.0%
CATCATAACCGAAGTTGTGA+GGG - chr4.2:84441759-84441778 None:intergenic 40.0%
CCAAAATGGACCAAAGTTTC+TGG + chr4.2:84441425-84441444 MS.gene70037:CDS 40.0%
CCAAGTTTAAAGGTTCGGAA+AGG + chr4.2:84441947-84441966 MS.gene70037:intron 40.0%
CCTTTCCGAACCTTTAAACT+TGG - chr4.2:84441950-84441969 None:intergenic 40.0%
GAAGTCAACAAAATCACCGT+TGG - chr4.2:84442392-84442411 None:intergenic 40.0%
GATAAATGCCCTCACAACTT+CGG + chr4.2:84441748-84441767 MS.gene70037:intron 40.0%
GCTTTCCACAAGTGCAATAA+AGG - chr4.2:84441341-84441360 None:intergenic 40.0%
GGATTGAGTAACACAGAAAC+AGG - chr4.2:84442523-84442542 None:intergenic 40.0%
GGTGTGTATCAGTTAGTGTT+AGG + chr4.2:84441869-84441888 MS.gene70037:intron 40.0%
GTAACACAGAAACAGGGTTA+AGG - chr4.2:84442516-84442535 None:intergenic 40.0%
GTAGTAGTACTAGGGTTTGA+TGG + chr4.2:84441848-84441867 MS.gene70037:intron 40.0%
GTGATATATGCAGAAGCCAA+CGG + chr4.2:84442373-84442392 MS.gene70037:CDS 40.0%
GTTAGATTAATGCCTTGCAC+CGG + chr4.2:84442298-84442317 MS.gene70037:CDS 40.0%
TACAATGGATCCTAATGGCA+TGG - chr4.2:84442431-84442450 None:intergenic 40.0%
TCAACATTCCTCAGATTTCG+CGG + chr4.2:84442648-84442667 MS.gene70037:CDS 40.0%
TCAATCTGAGGAATGTTGAG+TGG - chr4.2:84442580-84442599 None:intergenic 40.0%
TCATCAGACACCAGAAACTT+TGG - chr4.2:84441438-84441457 None:intergenic 40.0%
TCTCCCAACAGGTCAAAAAT+AGG + chr4.2:84442805-84442824 MS.gene70037:CDS 40.0%
TGGGATTTGTGAAGAGAGAA+GGG + chr4.2:84442833-84442852 MS.gene70037:CDS 40.0%
TGTATTTGTGAGAGGCTCAT+TGG - chr4.2:84442221-84442240 None:intergenic 40.0%
TTACTTATGTTACCGGTGCA+AGG - chr4.2:84442313-84442332 None:intergenic 40.0%
TTCATGCACAGTGATTCACA+TGG - chr4.2:84441234-84441253 None:intergenic 40.0%
TTCTTTACTCGGCGTGATAT+CGG + chr4.2:84443361-84443380 MS.gene70037:CDS 40.0%
TTGATGCAGATTGCTTAGGT+AGG - chr4.2:84442276-84442295 None:intergenic 40.0%
TTGATGTCAAGAATGCTCCA+AGG - chr4.2:84443085-84443104 None:intergenic 40.0%
! AACTCTTTTGCTGGATGTGT+TGG + chr4.2:84442466-84442485 MS.gene70037:CDS 40.0%
! ATCATTGTTGCTGTTGATGG+TGG - chr4.2:84441076-84441095 None:intergenic 40.0%
! ATTAACCTTGACTAGTGGAC+TGG + chr4.2:84443106-84443125 MS.gene70037:CDS 40.0%
! CAGGTCAAAAATAGGTGTTG+TGG + chr4.2:84442813-84442832 MS.gene70037:CDS 40.0%
! CCAGAAACTTTGGTCCATTT+TGG - chr4.2:84441428-84441447 None:intergenic 40.0%
! GATGATGGTACTCCAAACAA+TGG - chr4.2:84443572-84443591 None:intergenic 40.0%
! GTTTCTGTGTTACTCAATCC+TGG + chr4.2:84442523-84442542 MS.gene70037:CDS 40.0%
! TTGCAGCTGTTGATGGAATT+GGG + chr4.2:84441492-84441511 MS.gene70037:CDS 40.0%
! TTGGGATCTAACTCTTTTGC+TGG + chr4.2:84442457-84442476 MS.gene70037:CDS 40.0%
!! GAGAGTGTCAACAAAATCCT+TGG - chr4.2:84441007-84441026 None:intergenic 40.0%
!! GATAAGCATTGGTGAAGTTG+AGG + chr4.2:84442963-84442982 MS.gene70037:CDS 40.0%
!! TTAACCTTGACTAGTGGACT+GGG + chr4.2:84443107-84443126 MS.gene70037:CDS 40.0%
!! TTTCGAGGCTGTTGATTTGT+TGG - chr4.2:84441363-84441382 None:intergenic 40.0%
!!! GAATTCTTTTGGGGCAACAT+CGG + chr4.2:84443448-84443467 MS.gene70037:CDS 40.0%
AAAGACGGTATAGAAGCTGC+CGG - chr4.2:84443623-84443642 None:intergenic 45.0%
AATAGTGCGGAAGCCCAAAA+TGG + chr4.2:84441411-84441430 MS.gene70037:CDS 45.0%
AATGCAGAGGTGGATGATGA+AGG - chr4.2:84443233-84443252 None:intergenic 45.0%
ACCTACACTCAGACGATGTT+TGG + chr4.2:84441166-84441185 MS.gene70037:CDS 45.0%
AGCTTCCTATCTCTTCCACT+TGG + chr4.2:84441030-84441049 MS.gene70037:CDS 45.0%
AGTGATTCACATGGACTCCT+GGG - chr4.2:84441225-84441244 None:intergenic 45.0%
ATTTGTGAGAGGCTCATTGG+AGG - chr4.2:84442218-84442237 None:intergenic 45.0%
CAGGAACACCAAAGAGAAAG+AGG + chr4.2:84442623-84442642 MS.gene70037:CDS 45.0%
CCTCGAAATTTGGATTCGGA+GGG + chr4.2:84441375-84441394 MS.gene70037:CDS 45.0%
CCTTAATTGCACTGGAACCA+AGG + chr4.2:84442782-84442801 MS.gene70037:CDS 45.0%
CCTTGGTTCCAGTGCAATTA+AGG - chr4.2:84442785-84442804 None:intergenic 45.0%
CGGCTTCAAATACAGCTTCA+GGG + chr4.2:84443468-84443487 MS.gene70037:CDS 45.0%
CTTCGGTTATGATGCCATTG+TGG + chr4.2:84441765-84441784 MS.gene70037:intron 45.0%
GACTTACTTGCAGCTGTTGA+TGG + chr4.2:84441485-84441504 MS.gene70037:CDS 45.0%
GAGGAAGTCACACACAACAT+TGG + chr4.2:84441537-84441556 MS.gene70037:CDS 45.0%
GAGGATCCAAAATTGACGGA+AGG - chr4.2:84443341-84443360 None:intergenic 45.0%
GATCCAAAATTGACGGAAGG+AGG - chr4.2:84443338-84443357 None:intergenic 45.0%
GCATCATAACCGAAGTTGTG+AGG - chr4.2:84441760-84441779 None:intergenic 45.0%
GCCTTTGATGTTCCAAACGA+CGG - chr4.2:84443668-84443687 None:intergenic 45.0%
GGAACACCAAAGAGAAAGAG+GGG + chr4.2:84442625-84442644 MS.gene70037:CDS 45.0%
GGTGTACCAGAAGATGATGA+TGG - chr4.2:84443587-84443606 None:intergenic 45.0%
GTGGGATTTGTGAAGAGAGA+AGG + chr4.2:84442832-84442851 MS.gene70037:CDS 45.0%
GTTGCCCCAAAAGAATTCGT+AGG - chr4.2:84443446-84443465 None:intergenic 45.0%
TAAGCTGGGTGTTCAATCTG+AGG - chr4.2:84442592-84442611 None:intergenic 45.0%
TATCACGCCGAGTAAAGAAG+AGG - chr4.2:84443360-84443379 None:intergenic 45.0%
TCATCATCCACCTCTGCATT+TGG + chr4.2:84443233-84443252 MS.gene70037:CDS 45.0%
TCCAAACATCGTCTGAGTGT+AGG - chr4.2:84441170-84441189 None:intergenic 45.0%
TCGGCTTCAAATACAGCTTC+AGG + chr4.2:84443467-84443486 MS.gene70037:CDS 45.0%
TCGGCTTCTACACCATTGTT+TGG + chr4.2:84443557-84443576 MS.gene70037:CDS 45.0%
TCTGAGGAATGTTGAGTGGT+TGG - chr4.2:84442576-84442595 None:intergenic 45.0%
TGAAGCATGTTTCACCCTTC+AGG + chr4.2:84441821-84441840 MS.gene70037:intron 45.0%
TGCGAAGTCCTTAATTGCAC+TGG + chr4.2:84442774-84442793 MS.gene70037:CDS 45.0%
TGGATCCTAATGGCATGGTA+AGG - chr4.2:84442426-84442445 None:intergenic 45.0%
TGTTGACCCCTCTTTCTCTT+TGG - chr4.2:84442634-84442653 None:intergenic 45.0%
TTCAGACGCGACTCAATTAG+AGG + chr4.2:84441518-84441537 MS.gene70037:CDS 45.0%
! ACCGTCGTTTGGAACATCAA+AGG + chr4.2:84443664-84443683 MS.gene70037:CDS 45.0%
! CTTGCAGCTGTTGATGGAAT+TGG + chr4.2:84441491-84441510 MS.gene70037:CDS 45.0%
! GCAGCTTCTATACCGTCTTT+TGG + chr4.2:84443623-84443642 MS.gene70037:CDS 45.0%
! GGAATGTTGAGTGGTTGGTT+TGG - chr4.2:84442571-84442590 None:intergenic 45.0%
! GGAGTACCATCATCATCTTC+TGG + chr4.2:84443578-84443597 MS.gene70037:CDS 45.0%
! TCTTCTGGTACACCGTCTTT+TGG + chr4.2:84443593-84443612 MS.gene70037:CDS 45.0%
! TGCAGCTGTTGATGGAATTG+GGG + chr4.2:84441493-84441512 MS.gene70037:CDS 45.0%
! TGGTTTGGACAACCAAACTG+TGG - chr4.2:84442556-84442575 None:intergenic 45.0%
!! GTGTAGGCTTTGAACTTCCT+TGG + chr4.2:84443065-84443084 MS.gene70037:intron 45.0%
!! TGCTGTTGATGGTGGCTAAA+AGG - chr4.2:84441068-84441087 None:intergenic 45.0%
!! TGTTGATGGTGGCTAAAAGG+CGG - chr4.2:84441065-84441084 None:intergenic 45.0%
!!! ACCGTCTTTTGGAGCTCTAA+CGG + chr4.2:84443634-84443653 MS.gene70037:CDS 45.0%
!!! GTGATATCGGCTTCCCTTTT+CGG + chr4.2:84443374-84443393 MS.gene70037:CDS 45.0%
AAATAGCGAGCCAAAGGTGG+CGG - chr4.2:84443402-84443421 None:intergenic 50.0%
ACCAAATAGCGAGCCAAAGG+TGG - chr4.2:84443405-84443424 None:intergenic 50.0%
ACCACCAAATAGCGAGCCAA+AGG - chr4.2:84443408-84443427 None:intergenic 50.0%
ACCATTGTTTGGATCCCCAG+CGG + chr4.2:84443694-84443713 MS.gene70037:CDS 50.0%
AGGAGATGGCAAGAAAGAGG+AGG - chr4.2:84443300-84443319 None:intergenic 50.0%
AGTGTAGGTCTTGGACGGTT+TGG - chr4.2:84441155-84441174 None:intergenic 50.0%
CAGAGGTGGATGATGAAGGA+GGG - chr4.2:84443229-84443248 None:intergenic 50.0%
CAGTGATTCACATGGACTCC+TGG - chr4.2:84441226-84441245 None:intergenic 50.0%
CATCGTCTGAGTGTAGGTCT+TGG - chr4.2:84441164-84441183 None:intergenic 50.0%
CCAAACGGAGTTGAAGATGC+CGG - chr4.2:84443512-84443531 None:intergenic 50.0%
CCAAAGGACGGTACTCCAAA+CGG - chr4.2:84443527-84443546 None:intergenic 50.0%
CCATCTCCTTCCTCTTCTAG+AGG + chr4.2:84443311-84443330 MS.gene70037:CDS 50.0%
CCCTCCGAATCCAAATTTCG+AGG - chr4.2:84441378-84441397 None:intergenic 50.0%
CCTGGTATAGCACCACAGTT+TGG + chr4.2:84442541-84442560 MS.gene70037:CDS 50.0%
CTGTGAGGATCCAAATGCAG+AGG - chr4.2:84443246-84443265 None:intergenic 50.0%
GAGAGGCTCATTGGAGGTTA+AGG - chr4.2:84442212-84442231 None:intergenic 50.0%
GATGACGATGCTCCAAAGGA+CGG - chr4.2:84443539-84443558 None:intergenic 50.0%
GCCGTTAGAGCTCCAAAAGA+CGG - chr4.2:84443638-84443657 None:intergenic 50.0%
GCCTCGAAATTTGGATTCGG+AGG + chr4.2:84441374-84441393 MS.gene70037:CDS 50.0%
GGAAGGAGATGGCAAGAAAG+AGG - chr4.2:84443303-84443322 None:intergenic 50.0%
GGCAGAAGGAGAAGAAGTTG+CGG - chr4.2:84443279-84443298 None:intergenic 50.0%
GTCTGAGTGTAGGTCTTGGA+CGG - chr4.2:84441160-84441179 None:intergenic 50.0%
TGAGGATCCAAATGCAGAGG+TGG - chr4.2:84443243-84443262 None:intergenic 50.0%
TTTGACCTGTTGGGAGACCT+TGG - chr4.2:84442802-84442821 None:intergenic 50.0%
! ACCTTTGGCTCGCTATTTGG+TGG + chr4.2:84443404-84443423 MS.gene70037:CDS 50.0%
! CCTCTAGAAGAGGAAGGAGA+TGG - chr4.2:84443314-84443333 None:intergenic 50.0%
! GGACCTCCTTCCGTCAATTT+TGG + chr4.2:84443332-84443351 MS.gene70037:CDS 50.0%
! TGGTGCCAAGTGGAAGAGAT+AGG - chr4.2:84441038-84441057 None:intergenic 50.0%
!! AAAGGTGGCGGAACCGAAAA+GGG - chr4.2:84443390-84443409 None:intergenic 50.0%
!! CCAAACTGTGGTGCTATACC+AGG - chr4.2:84442544-84442563 None:intergenic 50.0%
!! GTCCTTTGGAGCATCGTCAT+CGG + chr4.2:84443538-84443557 MS.gene70037:CDS 50.0%
!!! TACACCGTCTTTTGGAGCAC+CGG + chr4.2:84443601-84443620 MS.gene70037:CDS 50.0%
ACAGCTTCAGGGTCGTCGTT+TGG + chr4.2:84443479-84443498 MS.gene70037:CDS 55.0%
ACGGCTTCTACACCGTCGTT+TGG + chr4.2:84443653-84443672 MS.gene70037:CDS 55.0%
AGCCGATGACGATGCTCCAA+AGG - chr4.2:84443543-84443562 None:intergenic 55.0%
AGGAGAAGAAGTTGCGGCAG+AGG - chr4.2:84443273-84443292 None:intergenic 55.0%
CCGGCATCTTCAACTCCGTT+TGG + chr4.2:84443509-84443528 MS.gene70037:CDS 55.0%
GCAGAGGTGGATGATGAAGG+AGG - chr4.2:84443230-84443249 None:intergenic 55.0%
GGCAAGAAAGAGGAGGCAGA+AGG - chr4.2:84443293-84443312 None:intergenic 55.0%
GGTGGCTAAAAGGCGGACAA+TGG - chr4.2:84441058-84441077 None:intergenic 55.0%
GTCTCCCAGTCCACTAGTCA+AGG - chr4.2:84443114-84443133 None:intergenic 55.0%
TGGAACCAAGGTCTCCCAAC+AGG + chr4.2:84442794-84442813 MS.gene70037:CDS 55.0%
! CTTTTCGGTTCCGCCACCTT+TGG + chr4.2:84443389-84443408 MS.gene70037:CDS 55.0%
! GCCACCTTTGGCTCGCTATT+TGG + chr4.2:84443401-84443420 MS.gene70037:CDS 55.0%
! GGAAGGAGGTCCTCTAGAAG+AGG - chr4.2:84443324-84443343 None:intergenic 55.0%
! GGAGGTCCTCTAGAAGAGGA+AGG - chr4.2:84443320-84443339 None:intergenic 55.0%
! GTCGTCGTTTGGAGCATCAC+CGG + chr4.2:84443490-84443509 MS.gene70037:CDS 55.0%
! TTGCGCCGTCGTTTGGAGTT+TGG + chr4.2:84443720-84443739 MS.gene70037:CDS 55.0%
!! CAAAGGTGGCGGAACCGAAA+AGG - chr4.2:84443391-84443410 None:intergenic 55.0%
!! CCGTTTGGAGTACCGTCCTT+TGG + chr4.2:84443524-84443543 MS.gene70037:CDS 55.0%
!! GGTCTTGGACGGTTTGGTAG+AGG - chr4.2:84441149-84441168 None:intergenic 55.0%
GCCGCTGGGGATCCAAACAA+TGG - chr4.2:84443698-84443717 None:intergenic 60.0%
GCTGCCGGTGCTCCAAAAGA+CGG - chr4.2:84443608-84443627 None:intergenic 60.0%
TGCGGCAGAGGTTGTCTGTG+AGG - chr4.2:84443261-84443280 None:intergenic 60.0%
! AGGCGGACAATGGTGCCAAG+TGG - chr4.2:84441048-84441067 None:intergenic 60.0%
AACGACGGCGCAAAAGCCGC+TGG - chr4.2:84443713-84443732 None:intergenic 65.0%
ACGACGGCGCAAAAGCCGCT+GGG - chr4.2:84443712-84443731 None:intergenic 65.0%
! GCGGCTTTTGCGCCGTCGTT+TGG + chr4.2:84443713-84443732 MS.gene70037:CDS 65.0%
CGACGGCGCAAAAGCCGCTG+GGG - chr4.2:84443711-84443730 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84440907 84443748 84440907 ID=MS.gene70037
chr4.2 mRNA 84440907 84443748 84440907 ID=MS.gene70037.t1;Parent=MS.gene70037
chr4.2 exon 84440907 84441569 84440907 ID=MS.gene70037.t1.exon1;Parent=MS.gene70037.t1
chr4.2 CDS 84440907 84441569 84440907 ID=cds.MS.gene70037.t1;Parent=MS.gene70037.t1
chr4.2 exon 84442187 84442984 84442187 ID=MS.gene70037.t1.exon2;Parent=MS.gene70037.t1
chr4.2 CDS 84442187 84442984 84442187 ID=cds.MS.gene70037.t1;Parent=MS.gene70037.t1
chr4.2 exon 84443071 84443748 84443071 ID=MS.gene70037.t1.exon3;Parent=MS.gene70037.t1
chr4.2 CDS 84443071 84443748 84443071 ID=cds.MS.gene70037.t1;Parent=MS.gene70037.t1
Gene Sequence

>MS.gene70037

ATGGCTTCTCAACACGAACAACAACAAGAGTCAAAAACCATTCCCCTTAAAATCATAGTAGACAAACAAAGCAACAAAGTTATTTTTCTGGAAGCAACCAAGGATTTTGTTGACACTCTCTTTAGCTTCCTATCTCTTCCACTTGGCACCATTGTCCGCCTTTTAGCCACCATCAACAGCAACAATGATCAACAAAACAAACCCTCAGAATCATCACTATTCCTTGAAAACATCAAAAACCTCTACCAAACCGTCCAAGACCTACACTCAGACGATGTTTGGAACACAACTTTTGTCAAACAAATGTTGCTGTATCCCAGGAGTCCATGTGAATCACTGTGCATGAAGATGTTCTTGAACGTTGATGACACTGAACCATTAAACAAGTTTTTTGTGTGTGATTCTTGTAACAAGTTCACTACTTTTCAAAACCTTTATTGCACTTGTGGAAAGCCAACAAATCAACAGCCTCGAAATTTGGATTCGGAGGGTCAAGAGAATAGTAATAGTGCGGAAGCCCAAAATGGACCAAAGTTTCTGGTGTCTGATGATTTGAAGATTGTTCCATGTTCGTCTGTGACTTACTTGCAGCTGTTGATGGAATTGGGGTATTCAGACGCGACTCAATTAGAGGAAGTCACACACAACATTGGAAAACAGGAGGTAATTAATTCTGTTGTGTGGTTTTTTGTTTGATATGTATCTATCTCACAAGTTGTATCATTACATTGTTTTTTTTTTACTTGCATAGCATCTTAGACCAATCTTAATCATGAATCTTCACCTCATTGTTTTAATTTGTGGTTACATTTATTGAGAACCTTTATTATGGTAAAATTTAGATAAATGCCCTCACAACTTCGGTTATGATGCCATTGTGGAAACCTCTAAAATCATATATTTGTGACTGCAATTGAAGCATGTTTCACCCTTCAGGAAATGTAGTAGTACTAGGGTTTGATGGTGTGTATCAGTTAGTGTTAGGAACCCGAAAATTGAATAGATCTTAAATTTCAAAGTTGCAACACTAGTAGCTTGTACCAAGTTTAAAGGTTCGGAAAGGAAAAATATTAATTAATATTCACAATCTCAGGATTTAATACTTGACAAGAAAAAGAGAATATAATAAAAAAAGAGTAGAATAGTGCAGTTAGGGAAGTCAATTAGTTAGATAAGGATATTGATCACTTTATTGAAAAGTTAATGAAGTTAATTTGTGATTAGTTACTTCCTTTTTTTTGTTCGAAAAAGGTATTCATCACTTCCTTTTTGATGTACAGATATTGAAACTACTCAAGTATACCTTAACCTCCAATGAGCCTCTCACAAATACAATTTTGGCAAGCAGCTCCAAAAATAAAGACAGCCTACCTAAGCAATCTGCATCAACAGTTAGATTAATGCCTTGCACCGGTAACATAAGTAAAATCAATGTTAAGGTAGTGCAAAGTAAATCTCAGAAAAAGGTGATATATGCAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTAGTTTCCTTACCATGCCATTAGGATCCATTGTAAAGCTTTTGGGATCTAACTCTTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGGACCTTAACCCTGTTTCTGTGTTACTCAATCCTGGTATAGCACCACAGTTTGGTTGTCCAAACCAACCACTCAACATTCCTCAGATTGAACACCCAGCTTATTATTATGGTACAGGAACACCAAAGAGAAAGAGGGGTCAACATTCCTCAGATTTCGCGGATTATAATGGTAATCGTATAATGATGGATTGTAGTGGTAATACTGTGATAGAAGAAAAGATAGAAGGAGGAGTGATTTCTAAGTCACGCGAATCAATTTATTTTGCGAAGTCCTTAATTGCACTGGAACCAAGGTCTCCCAACAGGTCAAAAATAGGTGTTGTGGGATTTGTGAAGAGAGAAGGGTTATATTGTGTAGGAGATGATCTAAAAGCAAAACCACTTTTTGTTCATTCGCATCTTTCATTTTTGAAAGAGTTGAGCCTTCCTCTTGATGATCTTGAAATGAAGCTGATAAGCATTGGTGAAGTTGAGGTAAAGATGCTATTATTTTATCACTAAGTTTATAAAAATTAATTGTGTTATTTTAACCTCTTTGATTTCCTTTACTTGTGGTGTAGGCTTTGAACTTCCTTGGAGCATTCTTGACATCAAAATTAACCTTGACTAGTGGACTGGGAGACCTTTCGAAACTGCCTAAAATCTCAAACCCCTTTTGCAATCGAACATTCAAAACCCTTCCTCAATTTCAAAACCAAAAGGAAAAAACTTCACACCCTCCTTCATCATCCACCTCTGCATTTGGATCCTCACAGACAACCTCTGCCGCAACTTCTTCTCCTTCTGCCTCCTCTTTCTTGCCATCTCCTTCCTCTTCTAGAGGACCTCCTTCCGTCAATTTTGGATCCTCTTCTTTACTCGGCGTGATATCGGCTTCCCTTTTCGGTTCCGCCACCTTTGGCTCGCTATTTGGTGGTAGAGCTCCTACTACTCCTACGAATTCTTTTGGGGCAACATCGGCTTCAAATACAGCTTCAGGGTCGTCGTTTGGAGCATCACCGGCATCTTCAACTCCGTTTGGAGTACCGTCCTTTGGAGCATCGTCATCGGCTTCTACACCATTGTTTGGAGTACCATCATCATCTTCTGGTACACCGTCTTTTGGAGCACCGGCAGCTTCTATACCGTCTTTTGGAGCTCTAACGGCTTCTACACCGTCGTTTGGAACATCAAAGGCTTCTACACCATTGTTTGGATCCCCAGCGGCTTTTGCGCCGTCGTTTGGAGTTTGGTTTTGA
Protein sequence

>MS.gene70037.t1

MASQHEQQQESKTIPLKIIVDKQSNKVIFLEATKDFVDTLFSFLSLPLGTIVRLLATINSNNDQQNKPSESSLFLENIKNLYQTVQDLHSDDVWNTTFVKQMLLYPRSPCESLCMKMFLNVDDTEPLNKFFVCDSCNKFTTFQNLYCTCGKPTNQQPRNLDSEGQENSNSAEAQNGPKFLVSDDLKIVPCSSVTYLQLLMELGYSDATQLEEVTHNIGKQEILKLLKYTLTSNEPLTNTILASSSKNKDSLPKQSASTVRLMPCTGNISKINVKVVQSKSQKKVIYAEANGDFVDFIFSFLTMPLGSIVKLLGSNSFAGCVGNLYKSVEDLNPVSVLLNPGIAPQFGCPNQPLNIPQIEHPAYYYGTGTPKRKRGQHSSDFADYNGNRIMMDCSGNTVIEEKIEGGVISKSRESIYFAKSLIALEPRSPNRSKIGVVGFVKREGLYCVGDDLKAKPLFVHSHLSFLKELSLPLDDLEMKLISIGEVEALNFLGAFLTSKLTLTSGLGDLSKLPKISNPFCNRTFKTLPQFQNQKEKTSHPPSSSTSAFGSSQTTSAATSSPSASSFLPSPSSSRGPPSVNFGSSSLLGVISASLFGSATFGSLFGGRAPTTPTNSFGATSASNTASGSSFGASPASSTPFGVPSFGASSSASTPLFGVPSSSSGTPSFGAPAASIPSFGALTASTPSFGTSKASTPLFGSPAAFAPSFGVWF