AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89798

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89798.t1 MTR_4g017610 91.789 475 39 0 1 475 1 475 0.0 886
MS.gene89798.t1 MTR_4g017410 61.446 498 149 9 8 479 4 484 0.0 563
MS.gene89798.t1 MTR_4g017440 60.400 500 172 6 4 479 5 502 0.0 560
MS.gene89798.t1 MTR_4g017830 56.972 502 189 7 3 479 2 501 0.0 542
MS.gene89798.t1 MTR_4g017550 62.236 474 151 10 27 477 3 471 0.0 541
MS.gene89798.t1 MTR_4g019340 58.368 478 166 8 12 468 37 502 9.18e-178 508
MS.gene89798.t1 MTR_4g017470 53.529 510 166 10 1 479 1 470 5.51e-163 470
MS.gene89798.t1 MTR_4g019310 38.386 508 266 17 1 479 1 490 3.36e-95 297
MS.gene89798.t1 MTR_7g066260 33.733 501 259 14 6 479 8 462 3.27e-73 239
MS.gene89798.t1 MTR_7g066340 33.333 507 253 17 3 479 2 453 4.10e-73 239
MS.gene89798.t1 MTR_7g058710 32.871 505 262 13 16 482 15 480 8.98e-73 238
MS.gene89798.t1 MTR_7g066570 35.098 510 263 19 4 479 5 480 2.56e-71 237
MS.gene89798.t1 MTR_7g066350 33.130 492 257 12 7 475 3 445 5.03e-71 233
MS.gene89798.t1 MTR_7g066430 32.731 498 282 17 16 479 7 485 3.69e-64 216
MS.gene89798.t1 MTR_7g066430 25.203 246 158 4 7 241 251 481 1.58e-13 73.2
MS.gene89798.t1 MTR_7g066410 32.692 520 272 16 20 482 17 515 5.26e-64 216
MS.gene89798.t1 MTR_7g066410 26.562 256 156 6 2 243 273 510 1.62e-13 73.2
MS.gene89798.t1 MTR_7g012680 32.207 503 252 15 4 479 5 445 7.76e-64 214
MS.gene89798.t1 MTR_7g066400 31.388 497 249 16 15 485 14 444 2.03e-62 210
MS.gene89798.t1 MTR_1g041315 32.649 487 251 17 9 463 10 451 6.13e-62 210
MS.gene89798.t1 MTR_7g066360 32.787 488 269 14 6 479 7 449 1.62e-59 203
MS.gene89798.t1 MTR_7g062280 31.213 503 281 16 16 481 13 487 1.88e-57 198
MS.gene89798.t1 MTR_7g063900 31.864 499 276 14 16 479 13 482 2.44e-56 195
MS.gene89798.t1 MTR_7g063660 32.577 485 285 15 16 481 12 473 2.66e-56 194
MS.gene89798.t1 MTR_7g066580 31.463 499 258 15 20 486 2 448 5.66e-56 193
MS.gene89798.t1 MTR_7g063840 30.600 500 290 16 6 481 3 469 1.57e-54 190
MS.gene89798.t1 MTR_7g062300 31.200 500 289 15 2 478 5 472 1.32e-53 187
MS.gene89798.t1 MTR_7g066290 32.056 496 245 14 6 479 8 433 2.29e-53 186
MS.gene89798.t1 MTR_7g063880 30.137 511 280 18 1 481 1 464 1.42e-52 184
MS.gene89798.t1 MTR_7g063890 30.972 494 290 14 16 481 30 500 2.43e-52 184
MS.gene89798.t1 MTR_7g063820 32.041 490 283 17 16 479 13 478 1.78e-51 182
MS.gene89798.t1 MTR_7g066310 30.515 485 205 15 16 479 10 383 2.31e-49 174
MS.gene89798.t1 MTR_7g063920 28.916 498 271 14 1 481 1 432 6.76e-48 171
MS.gene89798.t1 MTR_7g063830 30.200 500 270 18 1 481 1 440 8.30e-48 171
MS.gene89798.t1 MTR_2g029400 29.193 483 229 13 9 467 10 403 1.97e-45 164
MS.gene89798.t1 MTR_3g007740 30.480 479 270 15 16 479 12 442 2.29e-45 164
MS.gene89798.t1 MTR_3g065200 28.721 477 254 16 16 473 6 415 1.15e-42 157
MS.gene89798.t1 MTR_4g019240 59.350 123 48 1 1 123 1 121 6.73e-41 145
MS.gene89798.t1 MTR_4g019240 74.419 43 11 0 437 479 112 154 1.63e-11 63.5
MS.gene89798.t1 MTR_7g058700 31.768 362 165 12 19 346 525 838 7.15e-41 157
MS.gene89798.t1 MTR_7g063670 29.800 500 278 18 1 479 4 451 1.52e-40 152
MS.gene89798.t1 MTR_7g066480 29.536 474 253 14 16 479 19 421 1.23e-39 149
MS.gene89798.t1 MTR_7g066280 27.830 424 228 16 100 479 1 390 3.65e-35 136
MS.gene89798.t1 MTR_7g066490 31.056 322 187 9 16 331 19 311 2.91e-31 125
MS.gene89798.t1 MTR_1g014060 35.242 227 124 8 22 241 9 219 3.78e-28 115
MS.gene89798.t1 MTR_1g014060 33.684 190 102 4 300 480 211 385 2.68e-17 84.0
MS.gene89798.t1 MTR_7g066680 32.046 259 147 7 222 459 37 287 3.79e-26 109
MS.gene89798.t1 MTR_8g023240 22.869 481 313 14 13 471 37 481 3.22e-20 94.0
MS.gene89798.t1 MTR_7g062270 34.872 195 99 8 8 193 5 180 5.30e-20 92.0
MS.gene89798.t1 MTR_7g063720 28.151 238 134 5 259 481 13 228 4.67e-19 86.7
MS.gene89798.t1 MTR_8g445860 28.511 235 123 7 269 473 13 232 1.20e-17 82.8
MS.gene89798.t1 MTR_8g445860 28.049 246 144 8 11 243 11 236 2.89e-15 75.9
MS.gene89798.t1 MTR_0150s0010 30.568 229 131 9 268 473 9 232 2.05e-16 79.0
MS.gene89798.t1 MTR_0150s0010 28.870 239 144 7 16 243 13 236 5.05e-13 69.3
MS.gene89798.t1 MTR_1g115440 30.222 225 137 7 268 473 10 233 1.71e-15 76.3
MS.gene89798.t1 MTR_1g115440 27.615 239 147 7 16 243 14 237 4.63e-15 75.1
MS.gene89798.t1 MTR_6g006800 72.917 48 11 1 1 48 1 46 5.11e-14 67.4
MS.gene89798.t1 MTR_7g100420 27.500 280 157 8 222 475 134 393 2.08e-13 72.4
MS.gene89798.t1 MTR_7g063850 26.872 227 138 9 272 481 69 284 2.92e-13 70.9
MS.gene89798.t1 MTR_1g115455 28.205 234 124 8 269 473 9 227 1.38e-12 67.8
MS.gene89798.t1 MTR_1g115455 33.858 127 65 4 16 137 12 124 2.30e-12 67.0
MS.gene89798.t1 MTR_1g115435 25.523 239 147 6 9 247 3 210 9.27e-12 65.1
MS.gene89798.t1 MTR_1g115450 42.857 63 36 0 269 331 7 69 1.46e-11 62.4
MS.gene89798.t1 MTR_8g445920 37.500 72 45 0 260 331 1 72 4.04e-11 60.5
MS.gene89798.t1 MTR_8g044890 37.500 72 45 0 260 331 1 72 4.04e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89798.t1 AT5G01120 30.488 492 304 14 10 475 6 485 4.36e-55 192
MS.gene89798.t1 AT3G09140 30.099 505 299 16 7 475 3 489 3.96e-54 189
MS.gene89798.t1 AT3G09140 24.280 243 153 5 261 472 1 243 3.50e-12 68.9
MS.gene89798.t1 AT5G43240 31.148 488 298 14 16 475 12 489 1.91e-52 185
MS.gene89798.t1 AT5G43240 31.148 488 298 14 16 475 12 489 1.91e-52 185
MS.gene89798.t1 AT5G43240 31.148 488 298 14 16 475 12 489 1.91e-52 185
MS.gene89798.t1 AT5G01130 29.938 481 291 15 6 479 3 444 1.24e-50 179
MS.gene89798.t1 AT5G01150 28.655 513 302 13 7 485 3 485 4.28e-49 176
MS.gene89798.t1 AT5G01150 25.106 235 136 7 261 467 1 223 7.48e-11 64.7
MS.gene89798.t1 AT3G09140 28.518 533 299 17 7 475 3 517 2.45e-48 174
MS.gene89798.t1 AT3G09110 32.286 350 204 11 10 345 6 336 3.42e-45 161
MS.gene89798.t1 AT5G01140 29.652 489 280 15 7 475 3 447 1.13e-44 163
MS.gene89798.t1 AT5G37320 30.063 479 299 16 20 479 18 479 3.10e-38 145
MS.gene89798.t1 AT3G09120 32.121 330 189 10 10 323 3 313 4.46e-38 141
MS.gene89798.t1 AT3G09120 26.872 227 147 4 266 473 3 229 6.91e-15 75.9
MS.gene89798.t1 AT5G43240 28.319 339 222 7 158 475 7 345 8.61e-25 105

Find 89 sgRNAs with CRISPR-Local

Find 143 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCCTGATTATTATTATTA+TGG 0.175602 4.4:-83301980 MS.gene89798:CDS
CCACGTGACCAATATGTTAA+TGG 0.248131 4.4:-83302301 MS.gene89798:CDS
GGGTATTAAAATCTTTATAA+TGG 0.249437 4.4:+83302138 None:intergenic
CATCGTCCGAGTTGAGGTTT+TGG 0.282836 4.4:+83303343 None:intergenic
ACCTCAACTCGGACGATGTT+TGG 0.309591 4.4:-83303338 MS.gene89798:CDS
CAATAATAAGAGAAATAAAA+TGG 0.315012 4.4:-83302266 MS.gene89798:CDS
AAATCCTTAGGTTGTCTATT+AGG 0.320019 4.4:+83303141 None:intergenic
TTACTATTCCATTAACATAT+TGG 0.323766 4.4:+83302293 None:intergenic
GGATTTGGACTCTGAGGGTC+TGG 0.324672 4.4:-83303124 MS.gene89798:CDS
TGCAAAATTGTATTAGTAAG+AGG 0.327361 4.4:+83302349 None:intergenic
CACACATAAAACTTACTTGA+TGG 0.339007 4.4:+83303222 None:intergenic
TGATGTCGGTGGTTGCTAAA+AGG 0.350182 4.4:+83303433 None:intergenic
CTGGGGAATCATGCCTCTAA+TGG 0.352032 4.4:-83303105 MS.gene89798:CDS
TAATAGACAACCTAAGGATT+TGG 0.352431 4.4:-83303139 MS.gene89798:CDS
GGAATCCTTACCATTCCATT+AGG 0.362270 4.4:-83302163 MS.gene89798:CDS
ATACCATAATAATAATAATC+AGG 0.363692 4.4:+83301977 None:intergenic
TGTATCAAAGAATCCATTAG+AGG 0.366366 4.4:+83303092 None:intergenic
AACGTCTAAGTCCACTTGTC+AGG 0.369892 4.4:+83301561 None:intergenic
AAAATGTCGAGGGGTTGATT+TGG 0.376322 4.4:+83302013 None:intergenic
GCAAAGCAGATCTCAGAAAA+AGG 0.384785 4.4:-83302230 MS.gene89798:CDS
ATGCAGATGTTGCTGAAGTC+AGG 0.386929 4.4:-83303003 MS.gene89798:CDS
AAGAGGCTCATGAGAGGTTA+AGG 0.401681 4.4:+83302366 None:intergenic
TACTTGTGGTGTAGGCTTTG+CGG 0.426049 4.4:-83301616 MS.gene89798:intron
ATCTTTATAATGGATCCTAA+TGG 0.432878 4.4:+83302148 None:intergenic
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.4:-83303468 MS.gene89798:CDS
AATCCTACAGCACCTTCTCT+TGG 0.447362 4.4:+83301854 None:intergenic
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.4:+83303463 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4.4:+83302377 None:intergenic
ATCCTACAGCACCTTCTCTT+GGG 0.485199 4.4:+83301855 None:intergenic
GATTTGGACTCTGAGGGTCT+GGG 0.486663 4.4:-83303123 MS.gene89798:CDS
CACATGTATCAATTGTCAAT+CGG 0.498037 4.4:-83301898 MS.gene89798:CDS
CCCTCAGAGTCCAAATCCTT+AGG 0.498299 4.4:+83303129 None:intergenic
AACACATCCAGGAAAATTGT+TGG 0.509207 4.4:+83302117 None:intergenic
TCCTTAGGTTGTCTATTAGG+AGG 0.515531 4.4:+83303144 None:intergenic
AGCAACATTTGCTTACAGAA+AGG 0.516025 4.4:+83303309 None:intergenic
ACCTCCTAATAGACAACCTA+AGG 0.517583 4.4:-83303145 MS.gene89798:CDS
CCTTGTATAGCACCACACTT+TGG 0.521703 4.4:-83302040 MS.gene89798:CDS
CACACAAAACATTGGCAAGA+AGG 0.522739 4.4:-83302950 MS.gene89798:CDS
TTAATTCTTTCAGATATGAA+AGG 0.526183 4.4:+83301762 None:intergenic
ATGGAGTTCAGCAAGGAACT+TGG 0.529572 4.4:+83303024 None:intergenic
TACCATAATAATAATAATCA+GGG 0.534358 4.4:+83301978 None:intergenic
CCAAAGTGTGGTGCTATACA+AGG 0.535426 4.4:+83302040 None:intergenic
TCCAAACATCGTCCGAGTTG+AGG 0.536828 4.4:+83303337 None:intergenic
TACAAATTACCAACACATCC+AGG 0.541284 4.4:+83302106 None:intergenic
ACACATCCAGGAAAATTGTT+GGG 0.541913 4.4:+83302118 None:intergenic
GTCATCATTGCAGAAGCTGA+TGG 0.544526 4.4:-83302208 MS.gene89798:CDS
TTGTTCTTGTTGAGAAGTCA+TGG 0.547094 4.4:+83303593 None:intergenic
CGAAACCTCGACTGCACCTG+TGG 0.548497 4.4:-83303171 MS.gene89798:CDS
CTTCAAGATCATCAAGAGGA+AGG 0.549012 4.4:+83301738 None:intergenic
ATTTGGACTCTGAGGGTCTG+GGG 0.553375 4.4:-83303122 MS.gene89798:CDS
CCATTAACATATTGGTCACG+TGG 0.563853 4.4:+83302301 None:intergenic
TGATGATTATAATACAATAG+AGG 0.563983 4.4:-83301936 MS.gene89798:CDS
AGGATGGAGCAACTGAGACA+TGG 0.564407 4.4:+83302060 None:intergenic
TATAATGGATCCTAATGGAA+TGG 0.565520 4.4:+83302153 None:intergenic
TGCAGATGTTGCTGAAGTCA+GGG 0.569222 4.4:-83303002 MS.gene89798:CDS
TCTTGATGATCTTGAAGTGA+AGG 0.571298 4.4:-83301732 MS.gene89798:CDS
TGACTTCAGCAACATCTGCA+TGG 0.571318 4.4:+83303005 None:intergenic
TGGATCCTAATGGAATGGTA+AGG 0.572194 4.4:+83302158 None:intergenic
ACGTCTAAGTCCACTTGTCA+GGG 0.573102 4.4:+83301562 None:intergenic
AGGAAAGAGTTAGCAGCAAG+TGG 0.581274 4.4:+83301782 None:intergenic
GGATGGAGCAACTGAGACAT+GGG 0.582410 4.4:+83302061 None:intergenic
ACCTAAGGATTTGGACTCTG+AGG 0.588589 4.4:-83303130 MS.gene89798:CDS
AGCAAATGTTGCTGCATCCG+AGG 0.591888 4.4:-83303299 MS.gene89798:CDS
GAAGAGAGCAACATTATATG+TGG 0.598338 4.4:-83301828 MS.gene89798:CDS
TTGTCGATCATTGATGTCGG+TGG 0.600116 4.4:+83303422 None:intergenic
GAAGTGAAGGTGATTACCAT+TGG 0.600646 4.4:-83301719 MS.gene89798:CDS
AGTGATTCACATGAATTCCT+CGG 0.604741 4.4:+83303282 None:intergenic
TTCAGACTCAACACAACTAG+AGG 0.605328 4.4:-83302977 MS.gene89798:CDS
CCTTACCTCAGCTTCACCAA+TGG 0.608284 4.4:+83301703 None:intergenic
AACGTTTCCCCTCAAAATCA+CGG 0.610398 4.4:-83303556 MS.gene89798:CDS
ATTGAACTCGACCCAAGAGA+AGG 0.619174 4.4:-83301866 MS.gene89798:CDS
TTGATGTGAAGAATGCTCGA+AGG 0.619854 4.4:+83301591 None:intergenic
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4.4:+83302380 None:intergenic
TTCACTTCAAGATCATCAAG+AGG 0.629431 4.4:+83301734 None:intergenic
CCTAAGGATTTGGACTCTGA+GGG 0.632367 4.4:-83303129 MS.gene89798:CDS
AGAGCAACATTATATGTGGT+AGG 0.637042 4.4:-83301824 MS.gene89798:CDS
AGTGTGGTGCTATACAAGGA+TGG 0.638386 4.4:+83302044 None:intergenic
GATTACCATTGGTGAAGCTG+AGG 0.638424 4.4:-83301708 MS.gene89798:intron
GAGGAAGTCACACAAAACAT+TGG 0.640036 4.4:-83302958 MS.gene89798:CDS
GGTTGCTAAAAGGCGGACGA+TGG 0.651188 4.4:+83303443 None:intergenic
CATCTGCATGGAGTTCAGCA+AGG 0.654155 4.4:+83303017 None:intergenic
TTGTTGTCGATCATTGATGT+CGG 0.654674 4.4:+83303419 None:intergenic
ACAAAACATTGGCAAGAAGG+AGG 0.657225 4.4:-83302947 MS.gene89798:intron
GACCCAAGAGAAGGTGCTGT+AGG 0.664812 4.4:-83301857 MS.gene89798:CDS
AACCGTCCAAAACCTCAACT+CGG 0.667289 4.4:-83303349 MS.gene89798:CDS
ATTAGTAAGAGGCTCATGAG+AGG 0.684922 4.4:+83302360 None:intergenic
TGATTTGGACAACCAAAGTG+TGG 0.694943 4.4:+83302028 None:intergenic
TGTCGGTGGTTGCTAAAAGG+CGG 0.706045 4.4:+83303436 None:intergenic
AGGCGGACGATGGTACCAAG+TGG 0.729828 4.4:+83303453 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAAAAAATCTATGTAATA+GGG + chr4.4:83302259-83302278 None:intergenic 10.0%
!! AAAAAAAATCTATGTAATAG+GGG + chr4.4:83302258-83302277 None:intergenic 15.0%
!! ATACCATAATAATAATAATC+AGG + chr4.4:83303149-83303168 None:intergenic 15.0%
!! CAATAATAAGAGAAATAAAA+TGG - chr4.4:83302857-83302876 MS.gene89798:intron 15.0%
!! GAAAAAAAAATCTATGTAAT+AGG + chr4.4:83302260-83302279 None:intergenic 15.0%
!! TACCATAATAATAATAATCA+GGG + chr4.4:83303148-83303167 None:intergenic 15.0%
!! AAAAAAATCTATGTAATAGG+GGG + chr4.4:83302257-83302276 None:intergenic 20.0%
!! AAAGAATATGTTAGAATTAG+AGG - chr4.4:83302544-83302563 MS.gene89798:intron 20.0%
!! ATAAAATGTTAGAACACAAA+TGG + chr4.4:83302711-83302730 None:intergenic 20.0%
!! ATCTATATTACGGTAAAATA+CGG - chr4.4:83302352-83302371 MS.gene89798:CDS 20.0%
!! GGGTATTAAAATCTTTATAA+TGG + chr4.4:83302988-83303007 None:intergenic 20.0%
!! TGATGATTATAATACAATAG+AGG - chr4.4:83303187-83303206 MS.gene89798:CDS 20.0%
!! TTAATTCTTTCAGATATGAA+AGG + chr4.4:83303364-83303383 None:intergenic 20.0%
!! TTACTATTCCATTAACATAT+TGG + chr4.4:83302833-83302852 None:intergenic 20.0%
!! TTCCCTGATTATTATTATTA+TGG - chr4.4:83303143-83303162 MS.gene89798:CDS 20.0%
!!! GATTTTATTGACTTCATTTT+TGG - chr4.4:83302939-83302958 MS.gene89798:intron 20.0%
!!! TAATAATCAGGGAATTTTAA+AGG + chr4.4:83303137-83303156 None:intergenic 20.0%
!!! TTTGTTGTGAATCTATATTA+CGG - chr4.4:83302342-83302361 MS.gene89798:CDS 20.0%
! CTGCAATTACAATATTTGTT+AGG - chr4.4:83302434-83302453 MS.gene89798:intron 25.0%
! GAAATAGAACTTTGATAGAA+TGG + chr4.4:83302634-83302653 None:intergenic 25.0%
! TAATGTTATCTGTTAGTGAT+AGG - chr4.4:83302489-83302508 MS.gene89798:intron 25.0%
! TAGGATATATAGTTAGTACT+GGG - chr4.4:83302453-83302472 MS.gene89798:intron 25.0%
! TGCAAAATTGTATTAGTAAG+AGG + chr4.4:83302777-83302796 None:intergenic 25.0%
! TTAGGATATATAGTTAGTAC+TGG - chr4.4:83302452-83302471 MS.gene89798:intron 25.0%
!! TGTTTGATGTTTTCAAAGAA+TGG + chr4.4:83301745-83301764 None:intergenic 25.0%
!!! ATCTTTATAATGGATCCTAA+TGG + chr4.4:83302978-83302997 None:intergenic 25.0%
!!! ATTTTAAAGGAAAAATGTCG+AGG + chr4.4:83303124-83303143 None:intergenic 25.0%
!!! TTTTAAAGGAAAAATGTCGA+GGG + chr4.4:83303123-83303142 None:intergenic 25.0%
AACACAAATGGTAAGAATGA+AGG + chr4.4:83302699-83302718 None:intergenic 30.0%
CAAAACTGCATCTAAATCAT+AGG + chr4.4:83302326-83302345 None:intergenic 30.0%
CACACATAAAACTTACTTGA+TGG + chr4.4:83301904-83301923 None:intergenic 30.0%
CACATGTATCAATTGTCAAT+CGG - chr4.4:83303225-83303244 MS.gene89798:CDS 30.0%
TAATAGACAACCTAAGGATT+TGG - chr4.4:83301984-83302003 MS.gene89798:CDS 30.0%
TGTATCAAAGAATCCATTAG+AGG + chr4.4:83302034-83302053 None:intergenic 30.0%
TTTAAAGGAAAAATGTCGAG+GGG + chr4.4:83303122-83303141 None:intergenic 30.0%
! AAATCCTTAGGTTGTCTATT+AGG + chr4.4:83301985-83302004 None:intergenic 30.0%
! TATCTAAAACCATTACATCG+TGG - chr4.4:83302411-83302430 MS.gene89798:intron 30.0%
! TTAATACCCAACAATTTTCC+TGG - chr4.4:83302999-83303018 MS.gene89798:CDS 30.0%
!! TATAATGGATCCTAATGGAA+TGG + chr4.4:83302973-83302992 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG - chr4.4:83301600-83301619 MS.gene89798:CDS 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG - chr4.4:83302223-83302242 MS.gene89798:CDS 30.0%
AACACATCCAGGAAAATTGT+TGG + chr4.4:83303009-83303028 None:intergenic 35.0%
ACAAGTAAAGGGAATCAAAG+AGG + chr4.4:83303494-83303513 None:intergenic 35.0%
ACACATCCAGGAAAATTGTT+GGG + chr4.4:83303008-83303027 None:intergenic 35.0%
AGAGCAACATTATATGTGGT+AGG - chr4.4:83303299-83303318 MS.gene89798:CDS 35.0%
AGCAACATTTGCTTACAGAA+AGG + chr4.4:83301817-83301836 None:intergenic 35.0%
AGTGATTCACATGAATTCCT+CGG + chr4.4:83301844-83301863 None:intergenic 35.0%
CAAAGCTCGTAAAAAAAGTC+TGG + chr4.4:83303593-83303612 None:intergenic 35.0%
CTCTTTATTCAGAACTCTCT+CGG - chr4.4:83302653-83302672 MS.gene89798:intron 35.0%
GAAGAGAGCAACATTATATG+TGG - chr4.4:83303295-83303314 MS.gene89798:CDS 35.0%
TACAAATTACCAACACATCC+AGG + chr4.4:83303020-83303039 None:intergenic 35.0%
TCAAAAATTACCCTGACAAG+TGG - chr4.4:83303551-83303570 MS.gene89798:CDS 35.0%
TCTTTGATTCCCTTTACTTG+TGG - chr4.4:83303493-83303512 MS.gene89798:CDS 35.0%
TGCATCTAAATCATAGGTGA+AGG + chr4.4:83302320-83302339 None:intergenic 35.0%
TTCACTTCAAGATCATCAAG+AGG + chr4.4:83303392-83303411 None:intergenic 35.0%
TTGTTGTCGATCATTGATGT+CGG + chr4.4:83301707-83301726 None:intergenic 35.0%
TTTCACTTGCATATGACCTT+AGG - chr4.4:83302276-83302295 MS.gene89798:CDS 35.0%
! AACAATTTTCCTGGATGTGT+TGG - chr4.4:83303008-83303027 MS.gene89798:CDS 35.0%
! TCTTGATGATCTTGAAGTGA+AGG - chr4.4:83303391-83303410 MS.gene89798:CDS 35.0%
! TTTGTCTACCGTGATTTTGA+GGG + chr4.4:83301578-83301597 None:intergenic 35.0%
AAAGCCTACACCACAAGTAA+AGG + chr4.4:83303506-83303525 None:intergenic 40.0%
AACGTTTCCCCTCAAAATCA+CGG - chr4.4:83301567-83301586 MS.gene89798:CDS 40.0%
AAGCCTACACCACAAGTAAA+GGG + chr4.4:83303505-83303524 None:intergenic 40.0%
ACAAAACATTGGCAAGAAGG+AGG - chr4.4:83302176-83302195 MS.gene89798:CDS 40.0%
ACCTCCTAATAGACAACCTA+AGG - chr4.4:83301978-83301997 MS.gene89798:CDS 40.0%
AGGTTAAGGTATACTTCAGG+AGG + chr4.4:83302746-83302765 None:intergenic 40.0%
ATTAGTAAGAGGCTCATGAG+AGG + chr4.4:83302766-83302785 None:intergenic 40.0%
ATTCCCTTTACTTGTGGTGT+AGG - chr4.4:83303499-83303518 MS.gene89798:CDS 40.0%
CACACAAAACATTGGCAAGA+AGG - chr4.4:83302173-83302192 MS.gene89798:CDS 40.0%
CCACGTGACCAATATGTTAA+TGG - chr4.4:83302822-83302841 MS.gene89798:intron 40.0%
CCATTAACATATTGGTCACG+TGG + chr4.4:83302825-83302844 None:intergenic 40.0%
CTTCAAGATCATCAAGAGGA+AGG + chr4.4:83303388-83303407 None:intergenic 40.0%
GAAGTGAAGGTGATTACCAT+TGG - chr4.4:83303404-83303423 MS.gene89798:CDS 40.0%
GAGAGGTTAAGGTATACTTC+AGG + chr4.4:83302749-83302768 None:intergenic 40.0%
GAGGAAGTCACACAAAACAT+TGG - chr4.4:83302165-83302184 MS.gene89798:CDS 40.0%
GCAAAGCAGATCTCAGAAAA+AGG - chr4.4:83302893-83302912 MS.gene89798:intron 40.0%
GGAATCCTTACCATTCCATT+AGG - chr4.4:83302960-83302979 MS.gene89798:CDS 40.0%
TAATTGCAGCCACGATGTAA+TGG + chr4.4:83302423-83302442 None:intergenic 40.0%
TATCTCGACAACGACACTAA+TGG + chr4.4:83302393-83302412 None:intergenic 40.0%
TGGATCCTAATGGAATGGTA+AGG + chr4.4:83302968-83302987 None:intergenic 40.0%
TTCAGACTCAACACAACTAG+AGG - chr4.4:83302146-83302165 MS.gene89798:CDS 40.0%
TTGATGTGAAGAATGCTCGA+AGG + chr4.4:83303535-83303554 None:intergenic 40.0%
! AAAATGTCGAGGGGTTGATT+TGG + chr4.4:83303113-83303132 None:intergenic 40.0%
! CTATGTAATAGGGGGTATAG+TGG + chr4.4:83302249-83302268 None:intergenic 40.0%
! GTTTGTCTACCGTGATTTTG+AGG + chr4.4:83301579-83301598 None:intergenic 40.0%
! TCCTTAGGTTGTCTATTAGG+AGG + chr4.4:83301982-83302001 None:intergenic 40.0%
! TCTATTAGGAGGTTTTCCAC+AGG + chr4.4:83301971-83301990 None:intergenic 40.0%
! TGATTTGGACAACCAAAGTG+TGG + chr4.4:83303098-83303117 None:intergenic 40.0%
! TTGTCTACCGTGATTTTGAG+GGG + chr4.4:83301577-83301596 None:intergenic 40.0%
!! GAAGGATCTTGTTTCTACAG+TGG + chr4.4:83302302-83302321 None:intergenic 40.0%
AACCGTCCAAAACCTCAACT+CGG - chr4.4:83301774-83301793 MS.gene89798:CDS 45.0%
AACGTCTAAGTCCACTTGTC+AGG + chr4.4:83303565-83303584 None:intergenic 45.0%
AAGAGGCTCATGAGAGGTTA+AGG + chr4.4:83302760-83302779 None:intergenic 45.0%
AATCCTACAGCACCTTCTCT+TGG + chr4.4:83303272-83303291 None:intergenic 45.0%
ACCTAAGGATTTGGACTCTG+AGG - chr4.4:83301993-83302012 MS.gene89798:CDS 45.0%
ACGTCTAAGTCCACTTGTCA+GGG + chr4.4:83303564-83303583 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG - chr4.4:83301655-83301674 MS.gene89798:intron 45.0%
AGGAAAGAGTTAGCAGCAAG+TGG + chr4.4:83303344-83303363 None:intergenic 45.0%
ATCCTACAGCACCTTCTCTT+GGG + chr4.4:83303271-83303290 None:intergenic 45.0%
ATGCAGATGTTGCTGAAGTC+AGG - chr4.4:83302120-83302139 MS.gene89798:CDS 45.0%
ATGGAGTTCAGCAAGGAACT+TGG + chr4.4:83302102-83302121 None:intergenic 45.0%
ATTGAACTCGACCCAAGAGA+AGG - chr4.4:83303257-83303276 MS.gene89798:CDS 45.0%
CCTAAGGATTTGGACTCTGA+GGG - chr4.4:83301994-83302013 MS.gene89798:CDS 45.0%
CCTTGTATAGCACCACACTT+TGG - chr4.4:83303083-83303102 MS.gene89798:CDS 45.0%
GATTACCATTGGTGAAGCTG+AGG - chr4.4:83303415-83303434 MS.gene89798:CDS 45.0%
GGCAAGAAGGAGGTAACTAA+GGG - chr4.4:83302186-83302205 MS.gene89798:CDS 45.0%
GTCATCATTGCAGAAGCTGA+TGG - chr4.4:83302915-83302934 MS.gene89798:intron 45.0%
TACTTGTGGTGTAGGCTTTG+CGG - chr4.4:83303507-83303526 MS.gene89798:CDS 45.0%
TGACTTCAGCAACATCTGCA+TGG + chr4.4:83302121-83302140 None:intergenic 45.0%
TGATGTCGGTGGTTGCTAAA+AGG + chr4.4:83301693-83301712 None:intergenic 45.0%
TGCAGATGTTGCTGAAGTCA+GGG - chr4.4:83302121-83302140 MS.gene89798:CDS 45.0%
TGGCAAGAAGGAGGTAACTA+AGG - chr4.4:83302185-83302204 MS.gene89798:CDS 45.0%
TGGTACCAAGTGGCAAAGAT+AGG + chr4.4:83301663-83301682 None:intergenic 45.0%
TTGTCGATCATTGATGTCGG+TGG + chr4.4:83301704-83301723 None:intergenic 45.0%
!! AGTGTGGTGCTATACAAGGA+TGG + chr4.4:83303082-83303101 None:intergenic 45.0%
!! CCAAAGTGTGGTGCTATACA+AGG + chr4.4:83303086-83303105 None:intergenic 45.0%
!! CTTGTTTCTACAGTGGCCTA+AGG + chr4.4:83302295-83302314 None:intergenic 45.0%
!!! AGTTGAGGTTTTGGACGGTT+TGG + chr4.4:83301774-83301793 None:intergenic 45.0%
!!! GGTTTTGGACGGTTTGGTAA+AGG + chr4.4:83301768-83301787 None:intergenic 45.0%
!!! AATTTTAATATTGATTAATA+AGG + chr4.4:83302518-83302537 None:intergenic 5.0%
ACCTCAACTCGGACGATGTT+TGG - chr4.4:83301785-83301804 MS.gene89798:CDS 50.0%
AGCAAATGTTGCTGCATCCG+AGG - chr4.4:83301824-83301843 MS.gene89798:CDS 50.0%
AGGATGGAGCAACTGAGACA+TGG + chr4.4:83303066-83303085 None:intergenic 50.0%
CATCTGCATGGAGTTCAGCA+AGG + chr4.4:83302109-83302128 None:intergenic 50.0%
CCATTGGTGAAGCTGAGGTA+AGG - chr4.4:83303420-83303439 MS.gene89798:CDS 50.0%
CCCTCAGAGTCCAAATCCTT+AGG + chr4.4:83301997-83302016 None:intergenic 50.0%
CCTTACCTCAGCTTCACCAA+TGG + chr4.4:83303423-83303442 None:intergenic 50.0%
CTGGGGAATCATGCCTCTAA+TGG - chr4.4:83302018-83302037 MS.gene89798:CDS 50.0%
GGATGGAGCAACTGAGACAT+GGG + chr4.4:83303065-83303084 None:intergenic 50.0%
TCCAAACATCGTCCGAGTTG+AGG + chr4.4:83301789-83301808 None:intergenic 50.0%
TGTCGGTGGTTGCTAAAAGG+CGG + chr4.4:83301690-83301709 None:intergenic 50.0%
TTGGTGAAGCTGAGGTAAGG+TGG - chr4.4:83303423-83303442 MS.gene89798:CDS 50.0%
! ATTTGGACTCTGAGGGTCTG+GGG - chr4.4:83302001-83302020 MS.gene89798:CDS 50.0%
! GATTTGGACTCTGAGGGTCT+GGG - chr4.4:83302000-83302019 MS.gene89798:CDS 50.0%
! GGGGGTATAGTGGCCTAAAA+TGG + chr4.4:83302239-83302258 None:intergenic 50.0%
!! CATCGTCCGAGTTGAGGTTT+TGG + chr4.4:83301783-83301802 None:intergenic 50.0%
!!! GTCCGAGTTGAGGTTTTGGA+CGG + chr4.4:83301779-83301798 None:intergenic 50.0%
GGTTGCTAAAAGGCGGACGA+TGG + chr4.4:83301683-83301702 None:intergenic 55.0%
! GGATTTGGACTCTGAGGGTC+TGG - chr4.4:83301999-83302018 MS.gene89798:CDS 55.0%
! GTTTTCCACAGGTGCAGTCG+AGG + chr4.4:83301960-83301979 None:intergenic 55.0%
!! GACCCAAGAGAAGGTGCTGT+AGG - chr4.4:83303266-83303285 MS.gene89798:CDS 55.0%
AGGCGGACGATGGTACCAAG+TGG + chr4.4:83301673-83301692 None:intergenic 60.0%
CGAAACCTCGACTGCACCTG+TGG - chr4.4:83301952-83301971 MS.gene89798:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 83301532 83303613 83301532 ID=MS.gene89798
chr4.4 mRNA 83301532 83303613 83301532 ID=MS.gene89798.t1;Parent=MS.gene89798
chr4.4 exon 83302948 83303613 83302948 ID=MS.gene89798.t1.exon1;Parent=MS.gene89798.t1
chr4.4 CDS 83302948 83303613 83302948 ID=cds.MS.gene89798.t1;Parent=MS.gene89798.t1
chr4.4 exon 83301709 83302410 83301709 ID=MS.gene89798.t1.exon2;Parent=MS.gene89798.t1
chr4.4 CDS 83301709 83302410 83301709 ID=cds.MS.gene89798.t1;Parent=MS.gene89798.t1
chr4.4 exon 83301532 83301624 83301532 ID=MS.gene89798.t1.exon3;Parent=MS.gene89798.t1
chr4.4 CDS 83301532 83301624 83301532 ID=cds.MS.gene89798.t1;Parent=MS.gene89798.t1
Gene Sequence

>MS.gene89798

ATGACTTCTCAACAAGAACAACAAGAAGAGTCAAAAACGTTTCCCCTCAAAATCACGGTAGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGAGACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTCCGCCTTTTAGCAACCACCGACATCAATGATCGACAACAACTCTCAGAATCATCACCATTCTTTGAAAACATCAAACACCTTTACCAAACCGTCCAAAACCTCAACTCGGACGATGTTTGGAACAATCCTTTCTGTAAGCAAATGTTGCTGCATCCGAGGAATTCATGTGAATCACTGTGCATGAAGTTGTACTTGAATATTGATGATTCTGAACCATCAAGTAAGTTTTATGTGTGTGATTCTTGTAACAAGTTCACTACTTTTCGAAACCTCGACTGCACCTGTGGAAAACCTCCTAATAGACAACCTAAGGATTTGGACTCTGAGGGTCTGGGGAATCATGCCTCTAATGGATTCTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAAGATTGTGCCAAGTTCCTTGCTGAACTCCATGCAGATGTTGCTGAAGTCAGGGATTTCAGACTCAACACAACTAGAGGAAGTCACACAAAACATTGGCAAGAAGGAGGTAACTAAGGGTGTTGTGTTGTTTTGTTTGTTGTGTTTCCATTTTAGGCCACTATACCCCCTATTACATAGATTTTTTTTTCACTTGCATATGACCTTAGGCCACTGTAGAAACAAGATCCTTCACCTATGATTTAGATGCAGTTTTGTTGTGAATCTATATTACGGTAAAATACGGCTGTATTTGCAGTTGCCATTAGTGTCGTTGTCGAGATATCTAAAACCATTACATCGTGGCTGCAATTACAATATTTGTTAGGATATATAGTTAGTACTGGGATTTGACATAGTATAATGTTATCTGTTAGTGATAGGAAACCTTATTAATCAATATTAAAATTAAAAAAAAAGAATATGTTAGAATTAGAGGTGTCATTAGAAAATGTAACTAGTTAGAGAAGACTAGTAAATATACAGTGTACTCTTGTTGTGTTCCATTCTATCAAAGTTCTATTTCTCTTTATTCAGAACTCTCTCGGTTATAATTTCTAAACTCAAACCTTCATTCTTACCATTTGTGTTCTAACATTTTATACAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACTAATACAATTTTGCAAAGTAGCTCAAAGAACAATGACGAACCACGTGACCAATATGTTAATGGAATAGTAACGCGCAATAATAAGAGAAATAAAATGGATATTAAAGTATTGCAAAGCAGATCTCAGAAAAAGGTCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTGGAATCCTTACCATTCCATTAGGATCCATTATAAAGATTTTAATACCCAACAATTTTCCTGGATGTGTTGGTAATTTGTACAAGAGTGTGAAGAACTTGAATCCCATGTCTCAGTTGCTCCATCCTTGTATAGCACCACACTTTGGTTGTCCAAATCAACCCCTCGACATTTTTCCTTTAAAATTCCCTGATTATTATTATTATGGTATTCATCACAATGATGACGATGATGATTATAATACAATAGAGGAAGTGATTTCTAATTCACATGTATCAATTGTCAATCGGAGAAACGTGATTGAACTCGACCCAAGAGAAGGTGCTGTAGGATTTGTGAAGAGAGCAACATTATATGTGGTAGGAAATGATCTAGAAGTAAAACCACTTGCTGCTAACTCTTTCCTTTCATATCTGAAAGAATTAAGCCTTCCTCTTGATGATCTTGAAGTGAAGGTGATTACCATTGGTGAAGCTGAGGTAAGGTGGCTATTGTTTGATTACTGAATTTATTACTTTTTTCTCTTTTTTTAACCTCTTTGATTCCCTTTACTTGTGGTGTAGGCTTTGCGGTTCCTTCGAGCATTCTTCACATCAAAAATTACCCTGACAAGTGGACTTAGACGTTTTTTACCAGACTTTTTTTACGAGCTTTGA
Protein sequence

>MS.gene89798.t1

MTSQQEQQEESKTFPLKITVDKQSNKVVFVEATKDFVETLFSFLSLPLGTIVRLLATTDINDRQQLSESSPFFENIKHLYQTVQNLNSDDVWNNPFCKQMLLHPRNSCESLCMKLYLNIDDSEPSSKFYVCDSCNKFTTFRNLDCTCGKPPNRQPKDLDSEGLGNHASNGFFDTESEQMFLVSDDLKIVPSSLLNSMQMLLKSGISDSTQLEEVTQNIGKKEILNLLKYTLTSHEPLTNTILQSSSKNNDEPRDQYVNGIVTRNNKRNKMDIKVLQSRSQKKVIIAEADGDFIDFIFGILTIPLGSIIKILIPNNFPGCVGNLYKSVKNLNPMSQLLHPCIAPHFGCPNQPLDIFPLKFPDYYYYGIHHNDDDDDYNTIEEVISNSHVSIVNRRNVIELDPREGAVGFVKRATLYVVGNDLEVKPLAANSFLSYLKELSLPLDDLEVKVITIGEAEALRFLRAFFTSKITLTSGLRRFLPDFFYEL