AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene45986

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45986.t1 MTR_7g066430 84.770 499 75 1 1 499 1 498 0.0 862
MS.gene45986.t1 MTR_7g066410 51.881 505 219 5 4 486 10 512 5.29e-172 495
MS.gene45986.t1 MTR_7g066570 47.379 496 230 10 5 492 15 487 1.38e-137 409
MS.gene45986.t1 MTR_7g012680 44.172 489 221 9 4 491 14 451 2.41e-122 366
MS.gene45986.t1 MTR_7g058710 42.331 489 253 10 4 486 12 477 5.35e-121 363
MS.gene45986.t1 MTR_7g066260 42.505 487 237 9 4 486 15 462 1.15e-116 353
MS.gene45986.t1 MTR_7g066260 28.205 234 150 7 255 480 12 235 2.53e-16 82.0
MS.gene45986.t1 MTR_7g066340 41.718 489 232 11 4 486 12 453 5.76e-111 338
MS.gene45986.t1 MTR_1g041315 40.501 479 232 8 4 470 14 451 9.33e-109 332
MS.gene45986.t1 MTR_7g066580 40.281 499 232 12 10 493 1 448 1.87e-108 330
MS.gene45986.t1 MTR_7g066350 41.529 484 233 12 4 483 9 446 9.63e-107 326
MS.gene45986.t1 MTR_7g062280 39.053 507 254 10 4 486 10 485 7.46e-106 325
MS.gene45986.t1 MTR_7g062300 39.113 496 249 8 4 483 12 470 8.72e-101 311
MS.gene45986.t1 MTR_7g063660 38.540 493 263 8 4 486 9 471 3.57e-99 307
MS.gene45986.t1 MTR_7g063660 27.706 231 145 7 257 480 8 223 1.00e-13 73.9
MS.gene45986.t1 MTR_7g063900 40.771 493 262 12 4 486 10 482 1.17e-96 301
MS.gene45986.t1 MTR_7g063900 25.862 232 150 8 257 480 9 226 4.51e-14 74.7
MS.gene45986.t1 MTR_7g066400 38.776 490 225 13 2 482 10 433 2.04e-96 299
MS.gene45986.t1 MTR_7g066360 39.228 492 244 11 2 489 12 452 7.45e-96 299
MS.gene45986.t1 MTR_7g066290 39.228 492 217 11 4 486 15 433 3.82e-94 293
MS.gene45986.t1 MTR_7g063880 37.988 487 263 11 4 486 11 462 1.00e-91 288
MS.gene45986.t1 MTR_7g063820 38.211 492 281 9 5 491 11 484 1.94e-89 282
MS.gene45986.t1 MTR_7g063840 37.347 490 266 12 5 486 11 467 4.67e-89 281
MS.gene45986.t1 MTR_7g058700 38.277 499 191 11 16 499 531 927 1.60e-88 291
MS.gene45986.t1 MTR_7g063830 38.776 490 240 12 5 491 11 443 4.46e-88 278
MS.gene45986.t1 MTR_7g066480 48.844 346 145 9 4 336 16 342 1.94e-87 275
MS.gene45986.t1 MTR_7g066480 62.651 83 31 0 412 494 347 429 1.64e-24 106
MS.gene45986.t1 MTR_2g029400 37.342 474 212 10 4 475 14 404 6.95e-86 271
MS.gene45986.t1 MTR_3g007740 37.782 487 246 12 4 486 9 442 9.02e-85 269
MS.gene45986.t1 MTR_7g066490 46.588 337 154 7 3 336 15 328 7.07e-84 266
MS.gene45986.t1 MTR_7g066490 61.446 83 32 0 412 494 333 415 1.16e-23 103
MS.gene45986.t1 MTR_7g063920 36.049 491 235 9 4 486 11 430 5.17e-83 264
MS.gene45986.t1 MTR_4g019310 36.419 497 280 13 4 482 8 486 4.93e-82 263
MS.gene45986.t1 MTR_7g063890 38.677 499 263 12 4 486 27 498 8.01e-78 253
MS.gene45986.t1 MTR_7g063890 28.696 230 145 6 257 480 26 242 6.11e-19 90.1
MS.gene45986.t1 MTR_7g063670 37.628 489 245 9 5 486 16 451 2.37e-76 248
MS.gene45986.t1 MTR_3g065200 34.395 471 243 12 3 472 2 407 6.76e-76 246
MS.gene45986.t1 MTR_4g019340 34.545 495 267 13 5 474 39 501 2.91e-66 223
MS.gene45986.t1 MTR_4g019340 27.600 250 145 7 257 482 37 274 6.02e-11 65.1
MS.gene45986.t1 MTR_4g017830 33.858 508 295 14 5 488 13 503 1.41e-65 221
MS.gene45986.t1 MTR_4g017830 27.801 241 150 5 255 482 9 238 5.45e-14 74.7
MS.gene45986.t1 MTR_4g017440 32.347 507 303 14 5 488 15 504 8.50e-65 219
MS.gene45986.t1 MTR_7g066680 42.532 308 134 6 208 482 37 334 1.69e-63 210
MS.gene45986.t1 MTR_4g017610 32.932 498 276 17 7 483 16 476 5.57e-63 213
MS.gene45986.t1 MTR_4g017610 28.163 245 150 5 253 482 8 241 5.24e-16 80.9
MS.gene45986.t1 MTR_4g017410 31.298 524 304 16 1 496 1 496 1.37e-58 202
MS.gene45986.t1 MTR_4g017470 31.274 518 282 11 5 496 13 482 5.95e-55 192
MS.gene45986.t1 MTR_7g066280 33.179 431 220 13 83 486 1 390 6.18e-55 190
MS.gene45986.t1 MTR_1g014060 44.076 211 114 3 13 223 9 215 6.09e-52 181
MS.gene45986.t1 MTR_1g014060 42.439 205 93 4 288 491 210 390 6.99e-37 140
MS.gene45986.t1 MTR_1g014060 28.774 212 132 7 267 471 9 208 9.06e-16 79.7
MS.gene45986.t1 MTR_4g017550 32.860 493 277 19 18 482 3 469 8.04e-52 183
MS.gene45986.t1 MTR_7g066310 39.764 254 102 3 246 486 168 383 1.99e-47 169
MS.gene45986.t1 MTR_7g066310 46.809 141 73 2 4 143 7 146 3.23e-35 136
MS.gene45986.t1 MTR_7g063720 39.655 232 97 5 261 483 26 223 3.46e-41 148
MS.gene45986.t1 MTR_7g063720 28.261 230 129 7 2 225 21 220 2.60e-16 79.0
MS.gene45986.t1 MTR_7g100420 37.003 327 176 8 159 482 94 393 5.17e-38 144
MS.gene45986.t1 MTR_7g063850 35.294 272 147 9 238 504 46 293 4.10e-36 136
MS.gene45986.t1 MTR_7g062270 40.116 172 99 1 5 176 11 178 3.10e-35 136
MS.gene45986.t1 MTR_8g023240 27.181 493 291 18 5 478 38 481 1.18e-31 129
MS.gene45986.t1 MTR_8g445860 29.218 243 147 7 2 233 11 239 9.53e-18 83.2
MS.gene45986.t1 MTR_7g066390 52.055 73 35 0 414 486 50 122 2.07e-17 79.7
MS.gene45986.t1 MTR_7g063690 37.086 151 86 3 135 285 81 222 5.45e-17 80.9
MS.gene45986.t1 MTR_7g063690 62.500 48 18 0 5 52 13 60 1.27e-11 65.1
MS.gene45986.t1 MTR_4g019240 44.330 97 46 2 5 93 14 110 4.24e-16 77.0
MS.gene45986.t1 MTR_1g115440 29.461 241 143 9 4 230 11 238 1.20e-13 71.2
MS.gene45986.t1 MTR_0150s0010 26.721 247 142 6 4 230 10 237 2.65e-13 70.1
MS.gene45986.t1 MTR_2g067310 31.447 159 66 4 15 173 58 173 3.59e-13 69.7
MS.gene45986.t1 MTR_1g115455 27.273 253 138 9 2 233 7 234 4.74e-13 69.3
MS.gene45986.t1 MTR_7g063760 52.239 67 31 1 421 486 1 67 1.34e-12 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene45986.t1 AT5G01120 33.533 501 285 13 5 482 10 485 4.90e-61 209
MS.gene45986.t1 AT5G43240 31.673 502 297 13 5 482 10 489 7.68e-61 208
MS.gene45986.t1 AT5G43240 31.673 502 297 13 5 482 10 489 7.68e-61 208
MS.gene45986.t1 AT5G43240 31.673 502 297 13 5 482 10 489 7.68e-61 208
MS.gene45986.t1 AT5G01140 32.099 486 284 13 4 486 9 451 1.25e-60 207
MS.gene45986.t1 AT3G09140 31.862 521 299 17 2 492 7 501 2.15e-58 201
MS.gene45986.t1 AT3G09140 24.896 241 164 6 255 480 6 244 6.31e-12 68.2
MS.gene45986.t1 AT5G01150 30.814 516 319 11 4 505 9 500 2.88e-58 201
MS.gene45986.t1 AT3G09140 30.292 548 300 17 2 492 7 529 2.19e-55 194
MS.gene45986.t1 AT3G09110 35.395 291 180 5 4 293 9 292 6.08e-50 174
MS.gene45986.t1 AT5G01130 30.612 490 271 15 4 482 9 440 5.54e-49 175
MS.gene45986.t1 AT3G09120 35.423 319 185 9 4 312 6 313 9.90e-49 171
MS.gene45986.t1 AT3G09120 25.532 235 158 5 255 480 3 229 1.02e-12 69.7
MS.gene45986.t1 AT5G37320 29.264 516 299 12 3 491 8 484 3.85e-45 165
MS.gene45986.t1 AT5G43240 28.857 350 190 12 160 482 28 345 9.61e-23 100

Find 82 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATCTCGAAGAACAACAATTT+TGG 0.193641 7.2:+44336262 MS.gene45986:CDS
TATTCTTCCGTTGAATTCTT+TGG 0.260474 7.2:-44336315 None:intergenic
GGGGATTCCTTCTTCCGATT+TGG 0.309886 7.2:+44337613 MS.gene45986:CDS
AACTCTTTCGAAGTAATATA+TGG 0.319176 7.2:-44337425 None:intergenic
CTTACTTTCTCAATGTTAGA+TGG 0.325555 7.2:-44336201 None:intergenic
CGGGGTAGAACACATGTTAA+AGG 0.327870 7.2:+44337160 MS.gene45986:CDS
TTGAATTGGTGGGCAAGTTT+AGG 0.335304 7.2:-44337287 None:intergenic
CAAATTTAAAATGCAGTTGC+GGG 0.349657 7.2:+44336448 MS.gene45986:CDS
ACTGCCCAACCCTGTTGTAT+TGG 0.353634 7.2:+44336989 MS.gene45986:CDS
ATACAAGAGCATTCTTGAAT+TGG 0.356482 7.2:+44337214 MS.gene45986:CDS
TTGTCAAAATCCAATACAAC+AGG 0.360905 7.2:-44336999 None:intergenic
TCGTCTTCTGCTCTGACTAA+TGG 0.361646 7.2:+44337801 MS.gene45986:CDS
AAATTGTTGTTCTTCGAGAT+TGG 0.371839 7.2:-44336259 None:intergenic
CTAGTGACACAAAATATATT+AGG 0.371958 7.2:-44337350 None:intergenic
CCATGCATATTTAACTGCTC+TGG 0.374280 7.2:+44337397 MS.gene45986:CDS
AACATTGAGAAAGTAAGCTT+TGG 0.387986 7.2:+44336207 MS.gene45986:CDS
TTCAAGTGCAAGAAGATTCA+TGG 0.391251 7.2:-44336610 None:intergenic
TTCTGATTTAAAAGATAAGC+TGG 0.410605 7.2:+44337259 MS.gene45986:CDS
TCCTTACATTTCCCTTGGGC+GGG 0.415979 7.2:+44337141 MS.gene45986:CDS
TAGTTTCCTTACATTTCCCT+TGG 0.418561 7.2:+44337136 MS.gene45986:CDS
TTTGTGTCACTAGACATAAT+AGG 0.420484 7.2:+44337360 MS.gene45986:CDS
ATTGGTTACGGAGTTTGAAT+TGG 0.424103 7.2:-44337301 None:intergenic
TCAACCTTGACTTTAAGAAA+TGG 0.427676 7.2:-44337831 None:intergenic
TCTGCTCTGACTAATGGTCT+TGG 0.434350 7.2:+44337807 MS.gene45986:CDS
CAAAGGTGTGTTTGAGACTA+AGG 0.434805 7.2:-44336925 None:intergenic
TCAAGGAGGCAAAGATTTCA+TGG 0.435034 7.2:+44336119 MS.gene45986:CDS
TTGGAGCCACAGTCATCAAC+TGG 0.436173 7.2:+44337467 MS.gene45986:CDS
TGAGACACTTCTCAAAGTTA+AGG 0.436596 7.2:+44337851 MS.gene45986:CDS
TAAGGATGAGCTCAAAGTTA+AGG 0.447408 7.2:+44337869 MS.gene45986:CDS
CTTTCCTCCAAATCGGAAGA+AGG 0.453157 7.2:-44337620 None:intergenic
GATCATTCTTTCCTCCAAAT+CGG 0.457025 7.2:-44337627 None:intergenic
AAGCCTAGCAATTGTAGCTA+AGG 0.458301 7.2:-44336169 None:intergenic
GATTCCTTCTTCCGATTTGG+AGG 0.458978 7.2:+44337616 MS.gene45986:CDS
TTTCATCAAAGGGCAACAAT+TGG 0.465774 7.2:-44337319 None:intergenic
AGACATAATAGGAGAAGAAC+TGG 0.475136 7.2:+44337371 MS.gene45986:CDS
ACACAAAATATATTAGGATC+AGG 0.478516 7.2:-44337344 None:intergenic
AAATCCAATACAACAGGGTT+GGG 0.479589 7.2:-44336993 None:intergenic
GAGGAAAGAATGATCACCAT+TGG 0.483156 7.2:+44337635 MS.gene45986:CDS
AGTTTCCTTACATTTCCCTT+GGG 0.483205 7.2:+44337137 MS.gene45986:CDS
GGATCAGGCATTTCATCAAA+GGG 0.495430 7.2:-44337329 None:intergenic
AGGATCAGGCATTTCATCAA+AGG 0.497099 7.2:-44337330 None:intergenic
GGTTACGGAGTTTGAATTGG+TGG 0.510699 7.2:-44337298 None:intergenic
GTAACAAGAGTATCAGTTTG+AGG 0.511310 7.2:-44337901 None:intergenic
TTCCTTACATTTCCCTTGGG+CGG 0.512120 7.2:+44337140 MS.gene45986:CDS
AAAATCCAATACAACAGGGT+TGG 0.520441 7.2:-44336994 None:intergenic
GGGGTAGAACACATGTTAAA+GGG 0.523293 7.2:+44337161 MS.gene45986:CDS
TCAAATTTAAAATGCAGTTG+CGG 0.524990 7.2:+44336447 MS.gene45986:CDS
TGGTCCATTTCTTAAAGTCA+AGG 0.525498 7.2:+44337827 MS.gene45986:CDS
CAAAGGGCAACAATTGGTTA+CGG 0.525929 7.2:-44337313 None:intergenic
TCAAGTGCAAGAAGATTCAT+GGG 0.529660 7.2:-44336609 None:intergenic
TTCCCTTAGCTACAATTGCT+AGG 0.535607 7.2:+44336166 MS.gene45986:CDS
TCAGTCACCATGTAAAATGC+TGG 0.536470 7.2:-44337533 None:intergenic
TTACATGGTGACTGATGACT+TGG 0.536780 7.2:+44337541 MS.gene45986:CDS
CAGAGCAGAAGACGACATCA+AGG 0.539091 7.2:-44337793 None:intergenic
GTTACGGAGTTTGAATTGGT+GGG 0.541425 7.2:-44337297 None:intergenic
CCTTACATTTCCCTTGGGCG+GGG 0.543355 7.2:+44337142 MS.gene45986:CDS
TATGGTGATTGTGGTGGAAG+AGG 0.543911 7.2:+44337497 MS.gene45986:CDS
CCAGAGCAGTTAAATATGCA+TGG 0.547050 7.2:-44337397 None:intergenic
AAAGCATAACATCGATGTCT+CGG 0.547614 7.2:+44336350 MS.gene45986:CDS
AGTGGTCAACAAGCCACCAC+TGG 0.550325 7.2:-44336421 None:intergenic
CCCCGCCCAAGGGAAATGTA+AGG 0.553475 7.2:-44337142 None:intergenic
TCATCAACTGGCGAAGCGTA+TGG 0.554710 7.2:+44337479 MS.gene45986:CDS
AGCCTAGCAATTGTAGCTAA+GGG 0.571248 7.2:-44336168 None:intergenic
TGCATATTTAACTGCTCTGG+AGG 0.595252 7.2:+44337400 MS.gene45986:CDS
CATGTGTTCTACCCCGCCCA+AGG 0.595789 7.2:-44337153 None:intergenic
TGCTTACAAGTTTATGAACG+TGG 0.600738 7.2:+44336641 MS.gene45986:CDS
ATGTGTTCTACCCCGCCCAA+GGG 0.607536 7.2:-44337152 None:intergenic
TCTTCAACCAAAGAATTCAA+CGG 0.621903 7.2:+44336308 MS.gene45986:CDS
GGCGAAGCGTATGGTGATTG+TGG 0.624211 7.2:+44337488 MS.gene45986:CDS
AATACTGTCAATGAAAACGA+AGG 0.627418 7.2:+44337023 MS.gene45986:CDS
AATTCTCAGTGAAATTTGTG+TGG 0.632684 7.2:-44336061 None:intergenic
TAGCACGCTGTATGAAAGTG+TGG 0.633787 7.2:+44336236 MS.gene45986:CDS
ATACTGTCAATGAAAACGAA+GGG 0.638947 7.2:+44337024 MS.gene45986:CDS
ATGTTAAAGGGAAACTCATG+TGG 0.642490 7.2:+44337173 MS.gene45986:CDS
TGTAGTCGTCAATCCAGTGG+TGG 0.654643 7.2:+44336408 MS.gene45986:CDS
ATGACAGAAACAGATGTACA+TGG 0.658175 7.2:-44337572 None:intergenic
GATTGTAGTCGTCAATCCAG+TGG 0.678128 7.2:+44336405 MS.gene45986:CDS
GCATAACATCGATGTCTCGG+AGG 0.684769 7.2:+44336353 MS.gene45986:CDS
TGTCAAAATCCAATACAACA+GGG 0.693439 7.2:-44336998 None:intergenic
GCTTCGCCAGTTGATGACTG+TGG 0.702717 7.2:-44337473 None:intergenic
GAAGCGTATGGTGATTGTGG+TGG 0.710145 7.2:+44337491 MS.gene45986:CDS
CGTGGATGTCACTTTCAACG+AGG 0.739877 7.2:+44336659 MS.gene45986:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAAAGAAAATATTTATAACA+TGG - chr7.2:44336709-44336728 None:intergenic 10.0%
!!! ATTAAATACTAGTACATTTT+TGG + chr7.2:44336785-44336804 MS.gene45986:intron 15.0%
!! AGGGAAGAAAATAAAAATAA+AGG + chr7.2:44337043-44337062 MS.gene45986:CDS 20.0%
!!! AATAAAGTTTTATTTGCTCA+AGG + chr7.2:44336102-44336121 MS.gene45986:CDS 20.0%
!!! GAAGAAAAAATTGATAATGA+AGG + chr7.2:44336504-44336523 MS.gene45986:CDS 20.0%
!!! TATTTGTATTTTGTAGATAC+TGG + chr7.2:44336887-44336906 MS.gene45986:intron 20.0%
!!! TGCAAATAAAATTTTGTTGT+TGG - chr7.2:44337084-44337103 None:intergenic 20.0%
! AACTCTTTCGAAGTAATATA+TGG - chr7.2:44337428-44337447 None:intergenic 25.0%
! ACACAAAATATATTAGGATC+AGG - chr7.2:44337347-44337366 None:intergenic 25.0%
! CTAGTGACACAAAATATATT+AGG - chr7.2:44337353-44337372 None:intergenic 25.0%
! TTCTGATTTAAAAGATAAGC+TGG + chr7.2:44337259-44337278 MS.gene45986:CDS 25.0%
!! AATGGTTCATTTTATTCCTT+TGG + chr7.2:44337733-44337752 MS.gene45986:intron 25.0%
!! GCATTTTAAATTTGAGAAAG+TGG - chr7.2:44336442-44336461 None:intergenic 25.0%
!! TCAAATTTAAAATGCAGTTG+CGG + chr7.2:44336447-44336466 MS.gene45986:CDS 25.0%
!!! ATTTCATTTTTGAGTAAGCT+GGG + chr7.2:44337593-44337612 MS.gene45986:CDS 25.0%
!!! CGATGTTATGCTTTAATTTT+TGG - chr7.2:44336344-44336363 None:intergenic 25.0%
!!! CTTTGGATTTTTTTTTTGCA+GGG + chr7.2:44337750-44337769 MS.gene45986:intron 25.0%
AACATTGAGAAAGTAAGCTT+TGG + chr7.2:44336207-44336226 MS.gene45986:CDS 30.0%
AACTTCATTACACAACACAA+TGG + chr7.2:44337715-44337734 MS.gene45986:intron 30.0%
AATACTGTCAATGAAAACGA+AGG + chr7.2:44337023-44337042 MS.gene45986:CDS 30.0%
AATTCTCAGTGAAATTTGTG+TGG - chr7.2:44336064-44336083 None:intergenic 30.0%
AGCAAAAAAATGTCTGTCAA+AGG - chr7.2:44336945-44336964 None:intergenic 30.0%
ATACTGTCAATGAAAACGAA+GGG + chr7.2:44337024-44337043 MS.gene45986:CDS 30.0%
CCTGCAAAAAAAAAATCCAA+AGG - chr7.2:44337752-44337771 None:intergenic 30.0%
CTTACTTTCTCAATGTTAGA+TGG - chr7.2:44336204-44336223 None:intergenic 30.0%
TATTCTTCCGTTGAATTCTT+TGG - chr7.2:44336318-44336337 None:intergenic 30.0%
TCTTCAACCAAAGAATTCAA+CGG + chr7.2:44336308-44336327 MS.gene45986:CDS 30.0%
TGTCAAAATCCAATACAACA+GGG - chr7.2:44337001-44337020 None:intergenic 30.0%
TTGTCAAAATCCAATACAAC+AGG - chr7.2:44337002-44337021 None:intergenic 30.0%
! AAATTGTTGTTCTTCGAGAT+TGG - chr7.2:44336262-44336281 None:intergenic 30.0%
! AGTTTTTAATATGCGACGAT+TGG + chr7.2:44336382-44336401 MS.gene45986:CDS 30.0%
! ATCTCGAAGAACAACAATTT+TGG + chr7.2:44336262-44336281 MS.gene45986:CDS 30.0%
! ATGTGTTCCATTGAAGTTTT+TGG + chr7.2:44337448-44337467 MS.gene45986:CDS 30.0%
! CAAATTTAAAATGCAGTTGC+GGG + chr7.2:44336448-44336467 MS.gene45986:CDS 30.0%
! GTTTTTAATATGCGACGATT+GGG + chr7.2:44336383-44336402 MS.gene45986:CDS 30.0%
! TCAACCTTGACTTTAAGAAA+TGG - chr7.2:44337834-44337853 None:intergenic 30.0%
! TGCAGCAACTTTTTGTATTT+CGG + chr7.2:44336539-44336558 MS.gene45986:CDS 30.0%
! TTTGTGTCACTAGACATAAT+AGG + chr7.2:44337360-44337379 MS.gene45986:CDS 30.0%
!! ATACAAGAGCATTCTTGAAT+TGG + chr7.2:44337214-44337233 MS.gene45986:CDS 30.0%
!!! AAAGTTTTATTTGCTCAAGG+AGG + chr7.2:44336105-44336124 MS.gene45986:CDS 30.0%
!!! ATTTTTTTTTTGCAGGGTCT+TGG + chr7.2:44337756-44337775 MS.gene45986:intron 30.0%
!!! CATTTCATTTTTGAGTAAGC+TGG + chr7.2:44337592-44337611 MS.gene45986:CDS 30.0%
!!! CCTTTGGATTTTTTTTTTGC+AGG + chr7.2:44337749-44337768 MS.gene45986:intron 30.0%
!!! CTTTACCTCTTTTTTTCCAA+TGG - chr7.2:44337654-44337673 None:intergenic 30.0%
!!! TTTCATTTTTGAGTAAGCTG+GGG + chr7.2:44337594-44337613 MS.gene45986:CDS 30.0%
AAAATCCAATACAACAGGGT+TGG - chr7.2:44336997-44337016 None:intergenic 35.0%
AAAGCATAACATCGATGTCT+CGG + chr7.2:44336350-44336369 MS.gene45986:CDS 35.0%
AAATCCAATACAACAGGGTT+GGG - chr7.2:44336996-44337015 None:intergenic 35.0%
AGACATAATAGGAGAAGAAC+TGG + chr7.2:44337371-44337390 MS.gene45986:CDS 35.0%
AGTTTCCTTACATTTCCCTT+GGG + chr7.2:44337137-44337156 MS.gene45986:CDS 35.0%
ATGACAGAAACAGATGTACA+TGG - chr7.2:44337575-44337594 None:intergenic 35.0%
ATGTTAAAGGGAAACTCATG+TGG + chr7.2:44337173-44337192 MS.gene45986:CDS 35.0%
ATTGGTTACGGAGTTTGAAT+TGG - chr7.2:44337304-44337323 None:intergenic 35.0%
GATCACCATTGGAAAAAAAG+AGG + chr7.2:44337646-44337665 MS.gene45986:CDS 35.0%
GATCATTCTTTCCTCCAAAT+CGG - chr7.2:44337630-44337649 None:intergenic 35.0%
TAAGGATGAGCTCAAAGTTA+AGG + chr7.2:44337869-44337888 MS.gene45986:CDS 35.0%
TAGTTTCCTTACATTTCCCT+TGG + chr7.2:44337136-44337155 MS.gene45986:CDS 35.0%
TCAAGTGCAAGAAGATTCAT+GGG - chr7.2:44336612-44336631 None:intergenic 35.0%
TGAGACACTTCTCAAAGTTA+AGG + chr7.2:44337851-44337870 MS.gene45986:CDS 35.0%
TGCTTACAAGTTTATGAACG+TGG + chr7.2:44336641-44336660 MS.gene45986:CDS 35.0%
TGGTCCATTTCTTAAAGTCA+AGG + chr7.2:44337827-44337846 MS.gene45986:CDS 35.0%
TTCAAGTGCAAGAAGATTCA+TGG - chr7.2:44336613-44336632 None:intergenic 35.0%
TTTCATCAAAGGGCAACAAT+TGG - chr7.2:44337322-44337341 None:intergenic 35.0%
! GACAAGATCGTTTCTTTTGT+TGG + chr7.2:44336746-44336765 MS.gene45986:intron 35.0%
AAGCCTAGCAATTGTAGCTA+AGG - chr7.2:44336172-44336191 None:intergenic 40.0%
AGCCTAGCAATTGTAGCTAA+GGG - chr7.2:44336171-44336190 None:intergenic 40.0%
AGGATCAGGCATTTCATCAA+AGG - chr7.2:44337333-44337352 None:intergenic 40.0%
CAAAGGGCAACAATTGGTTA+CGG - chr7.2:44337316-44337335 None:intergenic 40.0%
CCAGAGCAGTTAAATATGCA+TGG - chr7.2:44337400-44337419 None:intergenic 40.0%
CCATGCATATTTAACTGCTC+TGG + chr7.2:44337397-44337416 MS.gene45986:CDS 40.0%
GAGGAAAGAATGATCACCAT+TGG + chr7.2:44337635-44337654 MS.gene45986:CDS 40.0%
GGATCAGGCATTTCATCAAA+GGG - chr7.2:44337332-44337351 None:intergenic 40.0%
GGGGTAGAACACATGTTAAA+GGG + chr7.2:44337161-44337180 MS.gene45986:CDS 40.0%
GTTACGGAGTTTGAATTGGT+GGG - chr7.2:44337300-44337319 None:intergenic 40.0%
TCAAGGAGGCAAAGATTTCA+TGG + chr7.2:44336119-44336138 MS.gene45986:CDS 40.0%
TCAGTCACCATGTAAAATGC+TGG - chr7.2:44337536-44337555 None:intergenic 40.0%
TGCATATTTAACTGCTCTGG+AGG + chr7.2:44337400-44337419 MS.gene45986:CDS 40.0%
TGTGGCTCCAAAAACTTCAA+TGG - chr7.2:44337458-44337477 None:intergenic 40.0%
TTACATGGTGACTGATGACT+TGG + chr7.2:44337541-44337560 MS.gene45986:CDS 40.0%
TTCCCTTAGCTACAATTGCT+AGG + chr7.2:44336166-44336185 MS.gene45986:CDS 40.0%
! CAAAGGTGTGTTTGAGACTA+AGG - chr7.2:44336928-44336947 None:intergenic 40.0%
! GAAGAGTCCAGCATTTTACA+TGG + chr7.2:44337526-44337545 MS.gene45986:CDS 40.0%
! TTGAATTGGTGGGCAAGTTT+AGG - chr7.2:44337290-44337309 None:intergenic 40.0%
!!! AGAAGCATTTTCTTACACGC+AGG - chr7.2:44336291-44336310 None:intergenic 40.0%
CGGGGTAGAACACATGTTAA+AGG + chr7.2:44337160-44337179 MS.gene45986:CDS 45.0%
CTTTCCTCCAAATCGGAAGA+AGG - chr7.2:44337623-44337642 None:intergenic 45.0%
GATTGTAGTCGTCAATCCAG+TGG + chr7.2:44336405-44336424 MS.gene45986:CDS 45.0%
GGTTACGGAGTTTGAATTGG+TGG - chr7.2:44337301-44337320 None:intergenic 45.0%
TAGCACGCTGTATGAAAGTG+TGG + chr7.2:44336236-44336255 MS.gene45986:CDS 45.0%
TCGTCTTCTGCTCTGACTAA+TGG + chr7.2:44337801-44337820 MS.gene45986:CDS 45.0%
TCTGCTCTGACTAATGGTCT+TGG + chr7.2:44337807-44337826 MS.gene45986:CDS 45.0%
TTCCTTACATTTCCCTTGGG+CGG + chr7.2:44337140-44337159 MS.gene45986:CDS 45.0%
! TATGGTGATTGTGGTGGAAG+AGG + chr7.2:44337497-44337516 MS.gene45986:CDS 45.0%
!! GATTCCTTCTTCCGATTTGG+AGG + chr7.2:44337616-44337635 MS.gene45986:CDS 45.0%
ACTGCCCAACCCTGTTGTAT+TGG + chr7.2:44336989-44337008 MS.gene45986:CDS 50.0%
CAGAGCAGAAGACGACATCA+AGG - chr7.2:44337796-44337815 None:intergenic 50.0%
GAAGCGTATGGTGATTGTGG+TGG + chr7.2:44337491-44337510 MS.gene45986:CDS 50.0%
GCATAACATCGATGTCTCGG+AGG + chr7.2:44336353-44336372 MS.gene45986:CDS 50.0%
GGGGATTCCTTCTTCCGATT+TGG + chr7.2:44337613-44337632 MS.gene45986:CDS 50.0%
TCATCAACTGGCGAAGCGTA+TGG + chr7.2:44337479-44337498 MS.gene45986:CDS 50.0%
TCCTTACATTTCCCTTGGGC+GGG + chr7.2:44337141-44337160 MS.gene45986:CDS 50.0%
TGTAGTCGTCAATCCAGTGG+TGG + chr7.2:44336408-44336427 MS.gene45986:CDS 50.0%
TTGGAGCCACAGTCATCAAC+TGG + chr7.2:44337467-44337486 MS.gene45986:CDS 50.0%
!! CGTGGATGTCACTTTCAACG+AGG + chr7.2:44336659-44336678 MS.gene45986:CDS 50.0%
AGTGGTCAACAAGCCACCAC+TGG - chr7.2:44336424-44336443 None:intergenic 55.0%
ATGTGTTCTACCCCGCCCAA+GGG - chr7.2:44337155-44337174 None:intergenic 55.0%
CCTTACATTTCCCTTGGGCG+GGG + chr7.2:44337142-44337161 MS.gene45986:CDS 55.0%
GGCGAAGCGTATGGTGATTG+TGG + chr7.2:44337488-44337507 MS.gene45986:CDS 55.0%
!! GCTTCGCCAGTTGATGACTG+TGG - chr7.2:44337476-44337495 None:intergenic 55.0%
CATGTGTTCTACCCCGCCCA+AGG - chr7.2:44337156-44337175 None:intergenic 60.0%
CCCCGCCCAAGGGAAATGTA+AGG - chr7.2:44337145-44337164 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr7.2 gene 44336057 44337911 44336057 ID=MS.gene45986
chr7.2 mRNA 44336057 44337911 44336057 ID=MS.gene45986.t1;Parent=MS.gene45986
chr7.2 exon 44336057 44336680 44336057 ID=MS.gene45986.t1.exon1;Parent=MS.gene45986.t1
chr7.2 CDS 44336057 44336680 44336057 ID=cds.MS.gene45986.t1;Parent=MS.gene45986.t1
chr7.2 exon 44336903 44337667 44336903 ID=MS.gene45986.t1.exon2;Parent=MS.gene45986.t1
chr7.2 CDS 44336903 44337667 44336903 ID=cds.MS.gene45986.t1;Parent=MS.gene45986.t1
chr7.2 exon 44337771 44337911 44337771 ID=MS.gene45986.t1.exon3;Parent=MS.gene45986.t1
chr7.2 CDS 44337771 44337911 44337771 ID=cds.MS.gene45986.t1;Parent=MS.gene45986.t1
Gene Sequence

>MS.gene45986

ATGGCCACACAAATTTCACTGAGAATTCTGATCGATGAAGAGAAAAATAAAGTTTTATTTGCTCAAGGAGGCAAAGATTTCATGGATATCATGCTTAGTTTCTTAACATTTCCCTTAGCTACAATTGCTAGGCTTGTTAACACGCCATCTAACATTGAGAAAGTAAGCTTTGGAAGCATTAGCACGCTGTATGAAAGTGTGGCCAATCTCGAAGAACAACAATTTTGGACACCTGCGTGTAAGAAAATGCTTCTTCAACCAAAGAATTCAACGGAAGAATATTGCCAAAAATTAAAGCATAACATCGATGTCTCGGAGGAAACTAAGTTTTTAATATGCGACGATTGGGATTGTAGTCGTCAATCCAGTGGTGGCTTGTTGACCACTTTCTCAAATTTAAAATGCAGTTGCGGGAGTTTTATGTCTCAATCGATATATCTTGCTGATGAAGAAAAAATTGATAATGAAGGATTTGTTGCAGATGCAGCAACTTTTTGTATTTCGGATGATTTAAGTGTGAAGTCTGATAGTTTTCAAAATTCTATTTGTGAACCCATGAATCTTCTTGCACTTGAAAACTTCAGTGCTTACAAGTTTATGAACGTGGATGTCACTTTCAACGAGGTATCTTTTTCTCTTACGCAAAATTCCATGTTATAAATATTTTCTTTAGTGAGTTTCTTGCTTTTGACAAGATCGTTTCTTTTGTTGGATCTTAATATTTTGCAATTAAATACTAGTACATTTTTGGAATTATTTATTAAAATAATGTGAATACTAGAATTTTATTAAAAAAATTGCTTCGTTTTAATGTAGTGCACTGCATTGTTTATTTGTATTTTGTAGATACTGGATTTGTTGAAGTGCTCCTTAGTCTCAAACACACCTTTGACAGACATTTTTTTGCTGAAGAAACAGAATTTTGAGAATTCACTGCCCAACCCTGTTGTATTGGATTTTGACAATAATACTGTCAATGAAAACGAAGGGAAGAAAATAAAAATAAAGGTAATGCTGCAAAAATCCAACAACAAAATTTTATTTGCATTAGCTGATGCTGATTTTGCAGACTTCATTCTTAGTTTCCTTACATTTCCCTTGGGCGGGGTAGAACACATGTTAAAGGGAAACTCATGTGGATCAAGCATAGACAATTTATACAAGAGCATTCTTGAATTGGACAGTGACACATACTTGAAATCTTCTGATTTAAAAGATAAGCTGGTTAAACCTAAACTTGCCCACCAATTCAAACTCCGTAACCAATTGTTGCCCTTTGATGAAATGCCTGATCCTAATATATTTTGTGTCACTAGACATAATAGGAGAAGAACTGGTTTCCATGCATATTTAACTGCTCTGGAGGATCCATATATTACTTCGAAAGAGTTATGTGTTCCATTGAAGTTTTTGGAGCCACAGTCATCAACTGGCGAAGCGTATGGTGATTGTGGTGGAAGAGGATTCATGAAGAGTCCAGCATTTTACATGGTGACTGATGACTTGGTTGTCACGCCATGTACATCTGTTTCTGTCATTTCATTTTTGAGTAAGCTGGGGATTCCTTCTTCCGATTTGGAGGAAAGAATGATCACCATTGGAAAAAAAGAGGTAAAGTAAAGCATGTTACTTTCACATTAGTATTCATTGTTTCAACAAACTTCATTACACAACACAATGGTTCATTTTATTCCTTTGGATTTTTTTTTTGCAGGGTCTTGGCATTTTGAAAGCTTCCTTGATGTCGTCTTCTGCTCTGACTAATGGTCTTGGTCCATTTCTTAAAGTCAAGGTTGAGACACTTCTCAAAGTTAAGGATGAGCTCAAAGTTAAGGATGAGAATACCTCAAACTGA
Protein sequence

>MS.gene45986.t1

MATQISLRILIDEEKNKVLFAQGGKDFMDIMLSFLTFPLATIARLVNTPSNIEKVSFGSISTLYESVANLEEQQFWTPACKKMLLQPKNSTEEYCQKLKHNIDVSEETKFLICDDWDCSRQSSGGLLTTFSNLKCSCGSFMSQSIYLADEEKIDNEGFVADAATFCISDDLSVKSDSFQNSICEPMNLLALENFSAYKFMNVDVTFNEILDLLKCSLVSNTPLTDIFLLKKQNFENSLPNPVVLDFDNNTVNENEGKKIKIKVMLQKSNNKILFALADADFADFILSFLTFPLGGVEHMLKGNSCGSSIDNLYKSILELDSDTYLKSSDLKDKLVKPKLAHQFKLRNQLLPFDEMPDPNIFCVTRHNRRRTGFHAYLTALEDPYITSKELCVPLKFLEPQSSTGEAYGDCGGRGFMKSPAFYMVTDDLVVTPCTSVSVISFLSKLGIPSSDLEERMITIGKKEGLGILKASLMSSSALTNGLGPFLKVKVETLLKVKDELKVKDENTSN