AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene84588

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84588.t1 MTR_7g066340 76.569 478 75 2 1 443 1 476 0.0 704
MS.gene84588.t1 MTR_7g066310 76.169 449 58 4 1 443 1 406 0.0 652
MS.gene84588.t1 MTR_7g066350 62.108 446 125 5 9 415 6 446 4.38e-180 511
MS.gene84588.t1 MTR_7g066280 61.785 437 59 4 91 443 1 413 8.39e-172 488
MS.gene84588.t1 MTR_1g041315 56.923 455 139 7 1 402 1 451 1.88e-165 475
MS.gene84588.t1 MTR_7g066260 54.582 491 163 8 6 443 9 492 9.16e-165 473
MS.gene84588.t1 MTR_7g066260 28.017 232 127 8 218 411 5 234 4.72e-14 74.3
MS.gene84588.t1 MTR_7g066400 55.629 453 144 8 1 415 1 434 8.95e-157 451
MS.gene84588.t1 MTR_7g066570 52.800 500 152 8 6 435 8 493 1.34e-151 442
MS.gene84588.t1 MTR_7g066360 52.567 487 165 10 6 442 8 478 3.04e-142 415
MS.gene84588.t1 MTR_7g066290 53.187 455 137 8 9 419 12 434 2.73e-137 401
MS.gene84588.t1 MTR_7g012680 50.216 464 158 7 6 420 8 447 4.78e-131 386
MS.gene84588.t1 MTR_7g058710 46.856 493 171 9 1 418 1 477 7.14e-127 376
MS.gene84588.t1 MTR_7g066580 47.033 455 173 12 18 418 1 441 1.03e-115 347
MS.gene84588.t1 MTR_7g066580 29.918 244 116 10 3 199 206 441 3.58e-14 74.7
MS.gene84588.t1 MTR_2g029400 47.936 436 152 9 6 406 8 403 4.57e-114 341
MS.gene84588.t1 MTR_7g066430 40.082 489 204 11 12 418 4 485 8.82e-104 318
MS.gene84588.t1 MTR_7g058700 44.643 448 138 8 24 418 531 921 1.17e-102 326
MS.gene84588.t1 MTR_7g066410 38.039 510 204 13 13 418 11 512 1.43e-93 292
MS.gene84588.t1 MTR_7g066410 30.544 239 109 8 12 201 284 514 2.45e-18 87.8
MS.gene84588.t1 MTR_7g066480 38.976 449 207 8 10 425 14 428 2.73e-93 288
MS.gene84588.t1 MTR_7g063920 38.657 432 230 6 11 418 10 430 8.04e-90 280
MS.gene84588.t1 MTR_7g063920 29.703 202 109 9 225 411 7 190 2.05e-13 72.0
MS.gene84588.t1 MTR_7g062300 37.657 478 225 12 1 414 1 469 1.04e-89 280
MS.gene84588.t1 MTR_7g066490 39.052 443 197 8 12 425 16 414 5.39e-89 277
MS.gene84588.t1 MTR_7g062280 37.113 485 212 12 11 414 9 481 1.68e-87 275
MS.gene84588.t1 MTR_7g063660 36.345 476 223 10 11 418 8 471 9.22e-84 265
MS.gene84588.t1 MTR_7g063900 39.212 482 205 11 12 416 10 480 1.53e-83 265
MS.gene84588.t1 MTR_7g063820 38.095 483 218 13 6 416 3 476 5.03e-83 263
MS.gene84588.t1 MTR_7g063880 37.500 472 214 14 11 419 10 463 1.39e-82 262
MS.gene84588.t1 MTR_7g063880 28.384 229 128 10 215 411 1 225 8.67e-12 67.4
MS.gene84588.t1 MTR_7g063840 37.238 478 221 14 1 414 1 463 3.15e-80 256
MS.gene84588.t1 MTR_7g063830 36.465 447 224 9 13 418 11 438 6.86e-78 249
MS.gene84588.t1 MTR_7g063890 37.810 484 214 11 11 418 26 498 4.90e-77 248
MS.gene84588.t1 MTR_7g063890 25.000 220 132 5 224 411 23 241 1.61e-13 72.8
MS.gene84588.t1 MTR_3g007740 37.174 460 212 14 11 418 8 442 5.05e-77 247
MS.gene84588.t1 MTR_7g063670 34.581 454 227 9 13 416 16 449 6.85e-70 229
MS.gene84588.t1 MTR_3g065200 34.348 460 186 10 10 405 1 408 1.47e-69 228
MS.gene84588.t1 MTR_4g017470 34.624 465 230 12 13 414 13 466 2.51e-69 228
MS.gene84588.t1 MTR_4g017470 28.444 225 124 7 220 424 4 211 2.82e-13 72.0
MS.gene84588.t1 MTR_4g017470 33.929 112 65 3 5 115 227 330 1.78e-11 66.2
MS.gene84588.t1 MTR_7g066680 46.823 299 96 5 180 415 37 335 3.61e-69 223
MS.gene84588.t1 MTR_4g017830 33.943 492 236 13 13 418 13 501 9.93e-69 228
MS.gene84588.t1 MTR_4g019310 32.323 495 233 13 9 414 5 486 3.11e-67 223
MS.gene84588.t1 MTR_4g019340 33.404 470 232 10 13 407 39 502 6.45e-67 222
MS.gene84588.t1 MTR_4g019340 29.730 222 111 8 6 192 291 502 1.88e-13 72.4
MS.gene84588.t1 MTR_4g017440 32.377 488 242 12 13 415 15 499 1.74e-63 213
MS.gene84588.t1 MTR_4g017410 32.649 487 227 13 13 414 10 480 2.99e-63 213
MS.gene84588.t1 MTR_4g017610 32.696 471 237 10 15 415 16 476 3.26e-63 211
MS.gene84588.t1 MTR_1g014060 50.685 219 68 4 21 203 9 223 6.45e-57 192
MS.gene84588.t1 MTR_1g014060 47.159 176 77 3 258 418 210 384 5.64e-36 137
MS.gene84588.t1 MTR_7g066390 51.124 178 53 3 269 442 1 148 5.80e-47 159
MS.gene84588.t1 MTR_4g017550 29.870 462 228 15 41 416 20 471 5.41e-40 149
MS.gene84588.t1 MTR_7g063720 34.579 214 123 3 220 418 15 226 1.68e-32 124
MS.gene84588.t1 MTR_7g063720 30.415 217 112 9 4 196 18 219 1.36e-17 82.4
MS.gene84588.t1 MTR_7g062270 38.596 171 70 2 13 149 11 180 3.12e-30 121
MS.gene84588.t1 MTR_7g063690 34.286 245 124 8 13 255 13 222 1.72e-29 115
MS.gene84588.t1 MTR_2g067310 46.853 143 62 4 23 161 58 190 1.05e-27 110
MS.gene84588.t1 MTR_7g100420 32.817 323 164 11 130 418 94 397 5.14e-25 106
MS.gene84588.t1 MTR_7g100420 57.627 59 21 1 13 67 40 98 1.49e-11 66.2
MS.gene84588.t1 MTR_8g023240 24.351 501 274 18 13 438 38 508 4.43e-24 105
MS.gene84588.t1 MTR_1g115440 31.356 236 111 5 11 202 10 238 6.30e-23 97.4
MS.gene84588.t1 MTR_7g063850 28.428 299 163 8 151 418 4 282 7.06e-23 98.6
MS.gene84588.t1 MTR_0150s0010 30.705 241 116 5 6 202 4 237 8.20e-21 91.3
MS.gene84588.t1 MTR_8g445860 29.876 241 121 5 4 203 5 238 4.26e-20 89.4
MS.gene84588.t1 MTR_1g115455 27.917 240 122 8 6 203 3 233 2.39e-15 75.5
MS.gene84588.t1 MTR_4g019240 38.393 112 57 4 5 108 6 113 1.59e-14 72.0
MS.gene84588.t1 MTR_1g115450 41.667 84 44 1 12 95 7 85 4.69e-14 69.7
MS.gene84588.t1 MTR_1g115470 42.857 84 43 1 12 95 6 84 9.36e-14 73.2
MS.gene84588.t1 MTR_0150s0030 39.362 94 51 2 6 98 2 90 2.21e-13 69.3
MS.gene84588.t1 MTR_8g044880 40.206 97 52 2 6 101 2 93 4.33e-13 69.7
MS.gene84588.t1 MTR_8g445910 40.426 94 50 2 6 98 2 90 1.69e-12 67.8
MS.gene84588.t1 MTR_8g445920 37.634 93 53 1 6 98 4 91 1.98e-12 63.9
MS.gene84588.t1 MTR_8g044890 37.634 93 53 1 6 98 4 91 1.98e-12 63.9
MS.gene84588.t1 MTR_7g063760 49.206 63 31 1 355 416 3 65 8.03e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene84588.t1 AT5G01120 29.537 518 257 14 13 442 10 507 2.56e-58 199
MS.gene84588.t1 AT3G09140 28.803 493 269 8 12 424 9 499 3.53e-53 186
MS.gene84588.t1 AT5G43240 29.942 521 255 14 13 442 10 511 8.82e-53 185
MS.gene84588.t1 AT5G43240 29.942 521 255 14 13 442 10 511 8.82e-53 185
MS.gene84588.t1 AT5G43240 29.942 521 255 14 13 442 10 511 8.82e-53 185
MS.gene84588.t1 AT5G01150 30.105 475 262 12 12 419 9 480 3.91e-51 180
MS.gene84588.t1 AT5G01140 29.218 486 267 12 12 442 9 472 1.80e-50 178
MS.gene84588.t1 AT3G09140 27.168 519 272 8 12 424 9 527 3.02e-49 176
MS.gene84588.t1 AT5G01130 31.858 452 247 15 12 418 9 444 1.41e-46 167
MS.gene84588.t1 AT5G01130 30.653 199 123 5 13 201 253 446 2.32e-12 68.9
MS.gene84588.t1 AT3G09110 30.938 320 177 6 12 293 9 322 7.80e-44 157
MS.gene84588.t1 AT3G09120 29.936 314 173 6 11 282 5 313 4.65e-34 130
MS.gene84588.t1 AT5G37320 26.596 470 261 16 19 415 18 476 5.09e-30 122
MS.gene84588.t1 AT5G43240 30.084 359 185 11 131 442 28 367 2.78e-28 115

Find 70 sgRNAs with CRISPR-Local

Find 146 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCATTTCCTCCACATCATTT+AGG 0.116915 7.3:-39463035 None:intergenic
TCTTGTCCACCAAAACTCTT+AGG 0.174508 7.3:-39461723 None:intergenic
GATCCTAAATCTCCTATTTC+TGG 0.199150 7.3:+39462907 MS.gene84588:CDS
AAGAAAACATACCTCTTTCT+TGG 0.258951 7.3:-39462313 None:intergenic
CCTCCAGAAATAGGAGATTT+AGG 0.263146 7.3:-39462910 None:intergenic
CATTTCCTCCACATCATTTA+GGG 0.283304 7.3:-39463034 None:intergenic
TGATCTTCTCAAATTGTCTT+TGG 0.284928 7.3:+39462441 MS.gene84588:CDS
CAAAGTAACCCTAAGAGTTT+TGG 0.291026 7.3:+39461714 MS.gene84588:CDS
ATTGAGTAGCATCTCCTTGC+AGG 0.299700 7.3:-39461941 None:intergenic
TAAGCGCCCGATGGGTTTAT+AGG 0.300844 7.3:-39461964 None:intergenic
TAGAGCACCATTAAAGATTA+TGG 0.313008 7.3:+39462939 MS.gene84588:CDS
CTAGCATATCCTCCAGAAAT+AGG 0.313219 7.3:-39462919 None:intergenic
TTAACCTCCATTTGGTTACT+TGG 0.318360 7.3:-39462526 None:intergenic
ACCCATCGGGCGCTTATTGC+AGG 0.321290 7.3:+39461971 MS.gene84588:CDS
AAACTTGTCGTAACAACATT+TGG 0.329105 7.3:-39462219 None:intergenic
CTTGGTGATGTTGATATTAA+CGG 0.332558 7.3:-39462295 None:intergenic
GTTTAGGTATCGGTAATATC+TGG 0.348563 7.3:-39462846 None:intergenic
TCTGTCACCATAATCTTTAA+TGG 0.356635 7.3:-39462946 None:intergenic
AATATTGAAGCAGTGAGATT+TGG 0.357605 7.3:+39461856 MS.gene84588:CDS
GAGGAAATGGTCGTTAATAT+TGG 0.365896 7.3:+39463048 MS.gene84588:CDS
TCAGATTGAATAGAAATCAT+TGG 0.402682 7.3:-39462814 None:intergenic
TTAAATCTCCTTCAGAAGCT+TGG 0.404186 7.3:+39462240 MS.gene84588:CDS
TATCAATATTAACCTCCATT+TGG 0.404727 7.3:-39462534 None:intergenic
AAAGGAACAATCTGATGAAA+TGG 0.409183 7.3:+39462561 MS.gene84588:CDS
AAGTGGAAGTAGATCTGTTC+AGG 0.414712 7.3:+39464118 MS.gene84588:CDS
TCAAAATCTGCCTTGACAAC+CGG 0.420103 7.3:+39463222 MS.gene84588:CDS
ACAGAGGTAAAATGACTTCA+AGG 0.422190 7.3:-39462025 None:intergenic
AAGATATGAAATACCATCAA+TGG 0.434003 7.3:-39462995 None:intergenic
CCTAAATGATGTGGAGGAAA+TGG 0.434219 7.3:+39463035 MS.gene84588:CDS
AATATGCAACACTGCTCCTA+AGG 0.452936 7.3:-39462680 None:intergenic
TCTATTTGCTGAGGCAGATA+AGG 0.457603 7.3:+39461759 MS.gene84588:CDS
CTTGTCCACCAAAACTCTTA+GGG 0.457678 7.3:-39461722 None:intergenic
TTATAATTGTGTTTAGGTAT+CGG 0.468696 7.3:-39462856 None:intergenic
TTCATATCTTGAAAGAATGA+AGG 0.484244 7.3:+39463008 MS.gene84588:CDS
AGCTTACAAAAGAGAATCCT+TGG 0.499942 7.3:-39462705 None:intergenic
TCCTGCAATAAGCGCCCGAT+GGG 0.500940 7.3:-39461972 None:intergenic
TGAGTTCAGACAAGTGTTTG+AGG 0.507323 7.3:+39462761 MS.gene84588:CDS
GGTTAATATTGATAAAGAAA+AGG 0.510314 7.3:+39462543 MS.gene84588:CDS
GATGCTTGCAATAGTACCTA+AGG 0.512187 7.3:-39461818 None:intergenic
TACTGTACTAACCTATGTAC+TGG 0.514263 7.3:-39463251 None:intergenic
TTGTCAACTTGTACAGCTGA+GGG 0.525848 7.3:-39461694 None:intergenic
CTCAATCCTATAAACCCATC+GGG 0.528295 7.3:+39461958 MS.gene84588:CDS
TATCAACATCACCAAGAAAG+AGG 0.532453 7.3:+39462302 MS.gene84588:CDS
TTTCTATTCAATCTGAACTC+AGG 0.535560 7.3:+39462821 MS.gene84588:CDS
GATTATGGTGACAGACGACT+TGG 0.540003 7.3:+39462954 MS.gene84588:CDS
ACTCCAATGTCATCCATTGA+TGG 0.546893 7.3:+39462982 MS.gene84588:CDS
ATATGCAACACTGCTCCTAA+GGG 0.554427 7.3:-39462679 None:intergenic
GGTCGTTAATATTGGCAAGA+AGG 0.557875 7.3:+39463056 MS.gene84588:CDS
CATTGACTCCAAGCTTCTGA+AGG 0.561296 7.3:-39462248 None:intergenic
ACCTATGTACTGGTTGAGAC+CGG 0.565014 7.3:-39463241 None:intergenic
CTGGTTGAGACCGGTTGTCA+AGG 0.565956 7.3:-39463232 None:intergenic
TTTGTCAACTTGTACAGCTG+AGG 0.572792 7.3:-39461695 None:intergenic
TTAAATATAAATTCGGACAG+AGG 0.575858 7.3:-39462475 None:intergenic
TTCCTGCAATAAGCGCCCGA+TGG 0.577842 7.3:-39461973 None:intergenic
ACTCAATCCTATAAACCCAT+CGG 0.584196 7.3:+39461957 MS.gene84588:CDS
AAATAAAGTTCTATTTGCTG+AGG 0.602370 7.3:+39461750 MS.gene84588:CDS
CAGAGGTAAAATGACTTCAA+GGG 0.603871 7.3:-39462024 None:intergenic
ATACCATCAATGGATGACAT+TGG 0.606080 7.3:-39462985 None:intergenic
CCTAAATCTCCTATTTCTGG+AGG 0.607923 7.3:+39462910 MS.gene84588:CDS
TGTTCCAAGTAACCAAATGG+AGG 0.609107 7.3:+39462522 MS.gene84588:CDS
GCAGTGTTGCATATTCTCCA+AGG 0.619271 7.3:+39462688 MS.gene84588:CDS
CGTTAATATTGGCAAGAAGG+AGG 0.619331 7.3:+39463059 MS.gene84588:CDS
ACCGGTCTCAACCAGTACAT+AGG 0.625446 7.3:+39463240 MS.gene84588:CDS
ATTTCCTCCACATCATTTAG+GGG 0.634248 7.3:-39463033 None:intergenic
TTTCCTCCACATCATTTAGG+GGG 0.636216 7.3:-39463032 None:intergenic
TCTACACTCACAAACCTGCA+AGG 0.640445 7.3:+39461927 MS.gene84588:CDS
ATGCTTGCAATAGTACCTAA+GGG 0.654856 7.3:-39461817 None:intergenic
TGCATACCGCATTCTCACAG+AGG 0.667726 7.3:-39462041 None:intergenic
TATGCAACACTGCTCCTAAG+GGG 0.682165 7.3:-39462678 None:intergenic
AGGTACTATTGCAAGCATCG+TGG 0.724958 7.3:+39461822 MS.gene84588:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTGCTTTTTAAATATAAATT+CGG - chr7.3:39462485-39462504 None:intergenic 10.0%
!!! TTTTGTTATTATTATAATGT+AGG - chr7.3:39463298-39463317 None:intergenic 10.0%
!! ATTTGTTTATATAACCATTA+AGG + chr7.3:39463605-39463624 MS.gene84588:intron 15.0%
!!! TTTTCATTATAATTGTGTTT+AGG - chr7.3:39462865-39462884 None:intergenic 15.0%
!! AACAAATCTAAGTGAATTAA+TGG - chr7.3:39463592-39463611 None:intergenic 20.0%
!! CTAAAAATTCCATTAAATTG+TGG - chr7.3:39463431-39463450 None:intergenic 20.0%
!! GGTTAATATTGATAAAGAAA+AGG + chr7.3:39462543-39462562 MS.gene84588:CDS 20.0%
!! TATTATTATAATGTAGGATG+AGG - chr7.3:39463292-39463311 None:intergenic 20.0%
!! TGAACTTTGGTTAAAAATAA+AGG + chr7.3:39463377-39463396 MS.gene84588:intron 20.0%
!! TGTACATGAACATATAATAA+AGG - chr7.3:39464091-39464110 None:intergenic 20.0%
!! TTATAATTGTGTTTAGGTAT+CGG - chr7.3:39462859-39462878 None:intergenic 20.0%
!! TTATTATATGTTCATGTACA+TGG + chr7.3:39464091-39464110 MS.gene84588:intron 20.0%
!! TTCTATCAAAATGTAATGAA+AGG + chr7.3:39463882-39463901 MS.gene84588:intron 20.0%
!! TTTGAGTAGATTAAAATAGT+CGG - chr7.3:39463642-39463661 None:intergenic 20.0%
!!! TAAAAATAAAGGACTAGTAT+TGG + chr7.3:39463388-39463407 MS.gene84588:intron 20.0%
! AAGATATGAAATACCATCAA+TGG - chr7.3:39462998-39463017 None:intergenic 25.0%
! AATGAAAGGTAACCATATAT+TGG + chr7.3:39463896-39463915 MS.gene84588:intron 25.0%
! ACAAGAGTTAAATATGTTGT+TGG - chr7.3:39463490-39463509 None:intergenic 25.0%
! AGGAATCAAAAATGTATTTG+TGG + chr7.3:39462096-39462115 MS.gene84588:intron 25.0%
! ATTTAGAGACCACAATTTAA+TGG + chr7.3:39463419-39463438 MS.gene84588:intron 25.0%
! CAACATATTTAACTCTTGTT+TGG + chr7.3:39463491-39463510 MS.gene84588:intron 25.0%
! CAACCACAAAAAATCAAATT+TGG - chr7.3:39463169-39463188 None:intergenic 25.0%
! GAGTGATTAAAAATGAAAGT+TGG + chr7.3:39463450-39463469 MS.gene84588:intron 25.0%
! GGAATCAAAAATGTATTTGT+GGG + chr7.3:39462097-39462116 MS.gene84588:intron 25.0%
! TATCAATATTAACCTCCATT+TGG - chr7.3:39462537-39462556 None:intergenic 25.0%
! TCAATATGAAAATAGTATGC+AGG - chr7.3:39463546-39463565 None:intergenic 25.0%
! TCAGATTGAATAGAAATCAT+TGG - chr7.3:39462817-39462836 None:intergenic 25.0%
! TGTTTATATAACCATTAAGG+AGG + chr7.3:39463608-39463627 MS.gene84588:intron 25.0%
! TTAAATATAAATTCGGACAG+AGG - chr7.3:39462478-39462497 None:intergenic 25.0%
! TTATAACATTTGAGAGTAGT+TGG + chr7.3:39463715-39463734 MS.gene84588:intron 25.0%
! TTCATATCTTGAAAGAATGA+AGG + chr7.3:39463008-39463027 MS.gene84588:CDS 25.0%
!! AAATAAAGTTCTATTTGCTG+AGG + chr7.3:39461750-39461769 MS.gene84588:CDS 25.0%
!! TTTTAATCTACTCAAATCGT+TGG + chr7.3:39463646-39463665 MS.gene84588:intron 25.0%
!!! AAAAATCCTGATTTTGAGTA+AGG + chr7.3:39462137-39462156 MS.gene84588:intron 25.0%
!!! ATGAAAGTTGGTATATTTAG+TGG + chr7.3:39463462-39463481 MS.gene84588:intron 25.0%
!!! TACTATCTTTAGAATTTGAC+TGG + chr7.3:39463515-39463534 MS.gene84588:intron 25.0%
!!! TCAAAATCAGGATTTTTTCT+CGG - chr7.3:39462134-39462153 None:intergenic 25.0%
!!! TCACCAAATTTGATTTTTTG+TGG + chr7.3:39463163-39463182 MS.gene84588:intron 25.0%
!!! TGAAAGTTGGTATATTTAGT+GGG + chr7.3:39463463-39463482 MS.gene84588:intron 25.0%
AAACTTGTCGTAACAACATT+TGG - chr7.3:39462222-39462241 None:intergenic 30.0%
AAAGGAACAATCTGATGAAA+TGG + chr7.3:39462561-39462580 MS.gene84588:CDS 30.0%
AAGAAAACATACCTCTTTCT+TGG - chr7.3:39462316-39462335 None:intergenic 30.0%
AATATTGAAGCAGTGAGATT+TGG + chr7.3:39461856-39461875 MS.gene84588:CDS 30.0%
AGAACTTCAGAATATTGTAC+AGG - chr7.3:39463772-39463791 None:intergenic 30.0%
CAGATATCGAAGAATTATTG+AGG - chr7.3:39462406-39462425 None:intergenic 30.0%
CTCAATAATTCTTCGATATC+TGG + chr7.3:39462404-39462423 MS.gene84588:intron 30.0%
GTTGAGAAAATGAGTAGTAA+AGG + chr7.3:39463963-39463982 MS.gene84588:intron 30.0%
TCATATGAGCATCCAATATA+TGG - chr7.3:39463911-39463930 None:intergenic 30.0%
TCTGTCACCATAATCTTTAA+TGG - chr7.3:39462949-39462968 None:intergenic 30.0%
TGATCTTCTCAAATTGTCTT+TGG + chr7.3:39462441-39462460 MS.gene84588:CDS 30.0%
TTTCTATTCAATCTGAACTC+AGG + chr7.3:39462821-39462840 MS.gene84588:CDS 30.0%
! TAGAGCACCATTAAAGATTA+TGG + chr7.3:39462939-39462958 MS.gene84588:CDS 30.0%
! TGTACTGTTTGAGTTACTAT+AGG + chr7.3:39462076-39462095 MS.gene84588:intron 30.0%
! TTTTGTTCCAAGTAACCAAA+TGG + chr7.3:39462519-39462538 MS.gene84588:CDS 30.0%
!! CTGATTTTGAGTAAGGAAAT+CGG + chr7.3:39462144-39462163 MS.gene84588:intron 30.0%
!! CTTGGTGATGTTGATATTAA+CGG - chr7.3:39462298-39462317 None:intergenic 30.0%
!!! ATTTGATTTTTTGTGGTTGC+AGG + chr7.3:39463170-39463189 MS.gene84588:intron 30.0%
!!! TTTGATTTTTTGTGGTTGCA+GGG + chr7.3:39463171-39463190 MS.gene84588:intron 30.0%
AAGAAAGAATGAACGAAAGG+TGG + chr7.3:39463986-39464005 MS.gene84588:intron 35.0%
ACAGAGGTAAAATGACTTCA+AGG - chr7.3:39462028-39462047 None:intergenic 35.0%
ACTCAATCCTATAAACCCAT+CGG + chr7.3:39461957-39461976 MS.gene84588:CDS 35.0%
AGAAAGAATGAACGAAAGGT+GGG + chr7.3:39463987-39464006 MS.gene84588:intron 35.0%
AGCTTACAAAAGAGAATCCT+TGG - chr7.3:39462708-39462727 None:intergenic 35.0%
AGGAAGAAAGAATGAACGAA+AGG + chr7.3:39463983-39464002 MS.gene84588:intron 35.0%
ATACCATCAATGGATGACAT+TGG - chr7.3:39462988-39463007 None:intergenic 35.0%
ATGCTTGCAATAGTACCTAA+GGG - chr7.3:39461820-39461839 None:intergenic 35.0%
ATTTCCTCCACATCATTTAG+GGG - chr7.3:39463036-39463055 None:intergenic 35.0%
CAGAGGTAAAATGACTTCAA+GGG - chr7.3:39462027-39462046 None:intergenic 35.0%
CATATGATCTAACGGTAATG+AGG + chr7.3:39463924-39463943 MS.gene84588:intron 35.0%
CATTTCCTCCACATCATTTA+GGG - chr7.3:39463037-39463056 None:intergenic 35.0%
CGATTTCCTTACTCAAAATC+AGG - chr7.3:39462146-39462165 None:intergenic 35.0%
GAGGAAATGGTCGTTAATAT+TGG + chr7.3:39463048-39463067 MS.gene84588:CDS 35.0%
GATCCTAAATCTCCTATTTC+TGG + chr7.3:39462907-39462926 MS.gene84588:CDS 35.0%
TACTGTACTAACCTATGTAC+TGG - chr7.3:39463254-39463273 None:intergenic 35.0%
TAGTACTACACGTAACAAGT+CGG - chr7.3:39463859-39463878 None:intergenic 35.0%
TATATGTTCATGTACATGGC+AGG + chr7.3:39464095-39464114 MS.gene84588:intron 35.0%
TATCAACATCACCAAGAAAG+AGG + chr7.3:39462302-39462321 MS.gene84588:CDS 35.0%
TGGATGCTCATATGATCTAA+CGG + chr7.3:39463916-39463935 MS.gene84588:intron 35.0%
TTAAATCTCCTTCAGAAGCT+TGG + chr7.3:39462240-39462259 MS.gene84588:CDS 35.0%
TTAACCTCCATTTGGTTACT+TGG - chr7.3:39462529-39462548 None:intergenic 35.0%
! AGCTTTTTAACATTGCCCTT+AGG + chr7.3:39461802-39461821 MS.gene84588:CDS 35.0%
! ATTTTACCTCTGTGAGAATG+CGG + chr7.3:39462035-39462054 MS.gene84588:CDS 35.0%
! CAAAGTAACCCTAAGAGTTT+TGG + chr7.3:39461714-39461733 MS.gene84588:CDS 35.0%
! CTTCGATATCTGGTTTGAAT+AGG + chr7.3:39462414-39462433 MS.gene84588:intron 35.0%
!! GAGTGTGTTAGTCTTCATTT+AGG + chr7.3:39463089-39463108 MS.gene84588:intron 35.0%
!! GTTTAGGTATCGGTAATATC+TGG - chr7.3:39462849-39462868 None:intergenic 35.0%
!! TATTCTGAAGTTCTGAAGTC+TGG + chr7.3:39463778-39463797 MS.gene84588:intron 35.0%
!! TGTTAGTCTTCATTTAGGAG+AGG + chr7.3:39463094-39463113 MS.gene84588:intron 35.0%
!!! ATGTAGGATGAGGTTTTGTT+TGG - chr7.3:39463282-39463301 None:intergenic 35.0%
!!! TTCTACTTTTGGCTTCTTCA+GGG - chr7.3:39464168-39464187 None:intergenic 35.0%
!!! TTTCTACTTTTGGCTTCTTC+AGG - chr7.3:39464169-39464188 None:intergenic 35.0%
AAGTGGAAGTAGATCTGTTC+AGG + chr7.3:39464118-39464137 MS.gene84588:CDS 40.0%
AATATGCAACACTGCTCCTA+AGG - chr7.3:39462683-39462702 None:intergenic 40.0%
ACTCCAATGTCATCCATTGA+TGG + chr7.3:39462982-39463001 MS.gene84588:CDS 40.0%
ATATGCAACACTGCTCCTAA+GGG - chr7.3:39462682-39462701 None:intergenic 40.0%
CCATTTCCTCCACATCATTT+AGG - chr7.3:39463038-39463057 None:intergenic 40.0%
CCTAAATGATGTGGAGGAAA+TGG + chr7.3:39463035-39463054 MS.gene84588:CDS 40.0%
CCTCCAGAAATAGGAGATTT+AGG - chr7.3:39462913-39462932 None:intergenic 40.0%
CGAACATGTCACATGAACTT+TGG + chr7.3:39463364-39463383 MS.gene84588:intron 40.0%
CGGTAATTTCTCCTCCTTAA+TGG - chr7.3:39463622-39463641 None:intergenic 40.0%
CGTTAATATTGGCAAGAAGG+AGG + chr7.3:39463059-39463078 MS.gene84588:CDS 40.0%
CTAGCATATCCTCCAGAAAT+AGG - chr7.3:39462922-39462941 None:intergenic 40.0%
CTCAATCCTATAAACCCATC+GGG + chr7.3:39461958-39461977 MS.gene84588:CDS 40.0%
CTTGTCCACCAAAACTCTTA+GGG - chr7.3:39461725-39461744 None:intergenic 40.0%
GATGCTTGCAATAGTACCTA+AGG - chr7.3:39461821-39461840 None:intergenic 40.0%
GGTCGTTAATATTGGCAAGA+AGG + chr7.3:39463056-39463075 MS.gene84588:CDS 40.0%
TCAAAATCTGCCTTGACAAC+CGG + chr7.3:39463222-39463241 MS.gene84588:CDS 40.0%
TCGGTTACTACTGATGTTAC+AGG - chr7.3:39463840-39463859 None:intergenic 40.0%
TCTAACGGTAATGAGGAGTA+AGG + chr7.3:39463931-39463950 MS.gene84588:intron 40.0%
TCTTGTCCACCAAAACTCTT+AGG - chr7.3:39461726-39461745 None:intergenic 40.0%
TGAGTTCAGACAAGTGTTTG+AGG + chr7.3:39462761-39462780 MS.gene84588:CDS 40.0%
TGTTCCAAGTAACCAAATGG+AGG + chr7.3:39462522-39462541 MS.gene84588:CDS 40.0%
TTCATGTGACATGTTCGAAG+TGG - chr7.3:39463361-39463380 None:intergenic 40.0%
TTGGCAAGAAGGAGGTAAAA+AGG + chr7.3:39463067-39463086 MS.gene84588:intron 40.0%
TTGTCAACTTGTACAGCTGA+GGG - chr7.3:39461697-39461716 None:intergenic 40.0%
TTTCCTCCACATCATTTAGG+GGG - chr7.3:39463035-39463054 None:intergenic 40.0%
TTTGTCAACTTGTACAGCTG+AGG - chr7.3:39461698-39461717 None:intergenic 40.0%
! AGTTTTCTCACTTTCCCCTT+AGG + chr7.3:39462664-39462683 MS.gene84588:CDS 40.0%
! TCTATTTGCTGAGGCAGATA+AGG + chr7.3:39461759-39461778 MS.gene84588:CDS 40.0%
!! AGTAACCCTAAGAGTTTTGG+TGG + chr7.3:39461717-39461736 MS.gene84588:CDS 40.0%
!! CCTAAATCTCCTATTTCTGG+AGG + chr7.3:39462910-39462929 MS.gene84588:CDS 40.0%
!!! TCTACTTTTGGCTTCTTCAG+GGG - chr7.3:39464167-39464186 None:intergenic 40.0%
AAGGAGAGTGAGAGTCATGA+AGG + chr7.3:39464014-39464033 MS.gene84588:intron 45.0%
AGAATGAACGAAAGGTGGGA+AGG + chr7.3:39463991-39464010 MS.gene84588:intron 45.0%
AGGAGAGTGAGAGTCATGAA+GGG + chr7.3:39464015-39464034 MS.gene84588:intron 45.0%
ATTGAGTAGCATCTCCTTGC+AGG - chr7.3:39461944-39461963 None:intergenic 45.0%
GATTATGGTGACAGACGACT+TGG + chr7.3:39462954-39462973 MS.gene84588:CDS 45.0%
GCAGTGTTGCATATTCTCCA+AGG + chr7.3:39462688-39462707 MS.gene84588:CDS 45.0%
TATGCAACACTGCTCCTAAG+GGG - chr7.3:39462681-39462700 None:intergenic 45.0%
TCTACACTCACAAACCTGCA+AGG + chr7.3:39461927-39461946 MS.gene84588:CDS 45.0%
TTCATGTACATGGCAGGAAG+TGG + chr7.3:39464101-39464120 MS.gene84588:intron 45.0%
! AGGTACTATTGCAAGCATCG+TGG + chr7.3:39461822-39461841 MS.gene84588:CDS 45.0%
! GGTTGTCAAGGCAGATTTTG+AGG - chr7.3:39463223-39463242 None:intergenic 45.0%
!! ACCTATGTACTGGTTGAGAC+CGG - chr7.3:39463244-39463263 None:intergenic 45.0%
!! CATTGACTCCAAGCTTCTGA+AGG - chr7.3:39462251-39462270 None:intergenic 45.0%
!!! CTACTTTTGGCTTCTTCAGG+GGG - chr7.3:39464166-39464185 None:intergenic 45.0%
ACCGGTCTCAACCAGTACAT+AGG + chr7.3:39463240-39463259 MS.gene84588:CDS 50.0%
GAAGGTCCCCCTAAATGATG+TGG + chr7.3:39463026-39463045 MS.gene84588:CDS 50.0%
TGAACGAAAGGTGGGAAGGA+AGG + chr7.3:39463995-39464014 MS.gene84588:intron 50.0%
TGCATACCGCATTCTCACAG+AGG - chr7.3:39462044-39462063 None:intergenic 50.0%
! TAAGCGCCCGATGGGTTTAT+AGG - chr7.3:39461967-39461986 None:intergenic 50.0%
CTGGTTGAGACCGGTTGTCA+AGG - chr7.3:39463235-39463254 None:intergenic 55.0%
GGTCCCCCTAAATGATGTGG+AGG + chr7.3:39463029-39463048 MS.gene84588:CDS 55.0%
TCCTGCAATAAGCGCCCGAT+GGG - chr7.3:39461975-39461994 None:intergenic 55.0%
TTCCTGCAATAAGCGCCCGA+TGG - chr7.3:39461976-39461995 None:intergenic 55.0%
ACCCATCGGGCGCTTATTGC+AGG + chr7.3:39461971-39461990 MS.gene84588:CDS 60.0%


Chromosome Type Strat End Strand Name
chr7.3 gene 39461682 39464193 39461682 ID=MS.gene84588
chr7.3 mRNA 39461682 39464193 39461682 ID=MS.gene84588.t1;Parent=MS.gene84588
chr7.3 exon 39461682 39462056 39461682 ID=MS.gene84588.t1.exon1;Parent=MS.gene84588.t1
chr7.3 CDS 39461682 39462056 39461682 ID=cds.MS.gene84588.t1;Parent=MS.gene84588.t1
chr7.3 exon 39462159 39462323 39462159 ID=MS.gene84588.t1.exon2;Parent=MS.gene84588.t1
chr7.3 CDS 39462159 39462323 39462159 ID=cds.MS.gene84588.t1;Parent=MS.gene84588.t1
chr7.3 exon 39462436 39463080 39462436 ID=MS.gene84588.t1.exon3;Parent=MS.gene84588.t1
chr7.3 CDS 39462436 39463080 39462436 ID=cds.MS.gene84588.t1;Parent=MS.gene84588.t1
chr7.3 exon 39463192 39463261 39463192 ID=MS.gene84588.t1.exon4;Parent=MS.gene84588.t1
chr7.3 CDS 39463192 39463261 39463192 ID=cds.MS.gene84588.t1;Parent=MS.gene84588.t1
chr7.3 exon 39464117 39464193 39464117 ID=MS.gene84588.t1.exon5;Parent=MS.gene84588.t1
chr7.3 CDS 39464117 39464193 39464117 ID=cds.MS.gene84588.t1;Parent=MS.gene84588.t1
Gene Sequence

>MS.gene84588

ATGACTGGAAATCCCTCAGCTGTACAAGTTGACAAAGTAACCCTAAGAGTTTTGGTGGACAAGAAGAAAAATAAAGTTCTATTTGCTGAGGCAGATAAGGATTTTGTTGATGTTCTTTTGAGCTTTTTAACATTGCCCTTAGGTACTATTGCAAGCATCGTGGCTAAAGAGTCAAATATTGAAGCAGTGAGATTTGGCAGCATTAGTTCACTCTATCAAAGTGTGTCAGTTCTTGATCAACGATATCTACACTCACAAACCTGCAAGGAGATGCTACTCAATCCTATAAACCCATCGGGCGCTTATTGCAGGAATATGAAACTGAACATTGACAATACTGAGCCCTTGAAGTCATTTTACCTCTGTGAGAATGCGGTATGCAAAATTGAAAATATGTACTGTTTGAGTTACTATAGGAATCAAAAATGTATTTGTGGGAAGCTGTTGAACCGAGAAAAAATCCTGATTTTGAGTAAGGAAATCGGTTTTGTTAATGAAACGTCGACGTTTATTGTATCTGATGATCTTTATGTTATGCCAAATGTTGTTACGACAAGTTTAAATCTCCTTCAGAAGCTTGGAGTCAATGACATTGATGCTATTGATAAACAAACCGTTAATATCAACATCACCAAGAAAGAGGTATGTTTTCTTATTCTGTGTTTGTATGACATATTTTTTGTTCATTATTATTATTATTATTATTATTTATTGTTTTTTGCCTCAATAATTCTTCGATATCTGGTTTGAATAGGTAGTTGATCTTCTCAAATTGTCTTTGGTCTCAAAAACTCCTCTGTCCGAATTTATATTTAAAAAGCAACAATCTGTTGAAATTTTTGTTCCAAGTAACCAAATGGAGGTTAATATTGATAAAGAAAAGGAACAATCTGATGAAATGGTTGTGAGAGTACTGAGAAGAAAATCAAATGAACAGATTTTGTTTGTTGAAGCAGATGATGATTTTGCTGATTTCATTTTTAGTTTTCTCACTTTCCCCTTAGGAGCAGTGTTGCATATTCTCCAAGGATTCTCTTTTGTAAGCTGCATTGATAATTTGTATAAGAGTGTGACTGAATTGAGTTCAGACAAGTGTTTGAGGTCACAACTTATGAAAGACAGACTAACGATTCCAATGATTTCTATTCAATCTGAACTCAGGATCCAGATATTACCGATACCTAAACACAATTATAATGAAAAAAGTATGCTACATAAATTTGCTGATCCTAAATCTCCTATTTCTGGAGGATATGCTAGAGCACCATTAAAGATTATGGTGACAGACGACTTGGTTGTGACTCCAATGTCATCCATTGATGGTATTTCATATCTTGAAAGAATGAAGGTCCCCCTAAATGATGTGGAGGAAATGGTCGTTAATATTGGCAAGAAGGAGGTAAAAAGGAGTGTGTTAGTCTTCATTTAGGAGAGGACACAGTCTTTCATTTTAATATTTCTTGTATTAGATTCATAACAACTCACCAAATTTGATTTTTTGTGGTTGCAGGGTCTCAGCATACTTAGAGCTTCATTGACCTCAAAATCTGCCTTGACAACCGGTCTCAACCAGTACATAGGTTAGTACAGTATTAGACCAAACAAAACCTCATCCTACATTATAATAATAACAAAAAATGATATTAAAGTTCATCGACAAAATTCATTTCAAACAGCCACTTCGAACATGTCACATGAACTTTGGTTAAAAATAAAGGACTAGTATTGGTAACAAAAATTTAGAGACCACAATTTAATGGAATTTTTAGAGTGATTAAAAATGAAAGTTGGTATATTTAGTGGGACCAACAACATATTTAACTCTTGTTTGGATACTATCTTTAGAATTTGACTGGTTGTGCCTGCATACTATTTTCATATTGAAGTACTTATGTAAACTAACATAGCCATTAATTCACTTAGATTTGTTTATATAACCATTAAGGAGGAGAAATTACCGACTATTTTAATCTACTCAAATCGTTGGTGCTACGCAATTTCTGCTTATAAAAATATTAGATAACTGCATTGAATTATAACATTTGAGAGTAGTTGGACTTTATATATTATACTCAATTTTTATGTTTCCTGTACAATATTCTGAAGTTCTGAAGTCTGGTAATTGTTTCTTTTCAATTCTCATCAACATTATTTTCCTGTAACATCAGTAGTAACCGACTTGTTACGTGTAGTACTAATTTTCTATCAAAATGTAATGAAAGGTAACCATATATTGGATGCTCATATGATCTAACGGTAATGAGGAGTAAGGACAGTTAATGTTGAGAAAATGAGTAGTAAAGGAAGAAAGAATGAACGAAAGGTGGGAAGGAAGGAGAGTGAGAGTCATGAAGGGAGTTTAATCTTGAATATGAAGTTAATAATTTTGAATATGTACTAAATACTCCTTTATTATATGTTCATGTACATGGCAGGAAGTGGAAGTAGATCTGTTCAGGCTAATTTGTCATCTAATGCTAGCCCCCTGAAGAAGCCAAAAGTAGAAAAATGA
Protein sequence

>MS.gene84588.t1

MTGNPSAVQVDKVTLRVLVDKKKNKVLFAEADKDFVDVLLSFLTLPLGTIASIVAKESNIEAVRFGSISSLYQSVSVLDQRYLHSQTCKEMLLNPINPSGAYCRNMKLNIDNTEPLKSFYLCENAEIGFVNETSTFIVSDDLYVMPNVVTTSLNLLQKLGVNDIDAIDKQTVNINITKKEVVDLLKLSLVSKTPLSEFIFKKQQSVEIFVPSNQMEVNIDKEKEQSDEMVVRVLRRKSNEQILFVEADDDFADFIFSFLTFPLGAVLHILQGFSFVSCIDNLYKSVTELSSDKCLRSQLMKDRLTIPMISIQSELRIQILPIPKHNYNEKSMLHKFADPKSPISGGYARAPLKIMVTDDLVVTPMSSIDGISYLERMKVPLNDVEEMVVNIGKKEGLSILRASLTSKSALTTGLNQYIGSGSRSVQANLSSNASPLKKPKVEK