AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene94743

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94743.t1 MTR_7g066480 92.791 430 30 1 1 429 1 430 0.0 801
MS.gene94743.t1 MTR_7g066490 82.870 432 55 4 1 429 1 416 0.0 695
MS.gene94743.t1 MTR_7g066260 42.266 459 204 10 16 422 15 464 4.41e-97 300
MS.gene94743.t1 MTR_7g066340 41.372 452 212 10 16 422 12 455 3.17e-96 297
MS.gene94743.t1 MTR_7g066340 27.982 218 124 8 14 229 261 447 3.34e-11 65.5
MS.gene94743.t1 MTR_7g066350 41.798 445 209 9 16 417 9 446 5.65e-93 288
MS.gene94743.t1 MTR_7g062300 37.021 470 217 9 16 417 12 470 2.99e-89 279
MS.gene94743.t1 MTR_7g066430 47.059 340 162 7 14 341 2 335 1.58e-88 278
MS.gene94743.t1 MTR_7g066430 53.125 96 43 1 325 420 392 485 5.44e-21 95.5
MS.gene94743.t1 MTR_7g066410 45.845 349 151 7 16 331 10 353 1.69e-88 278
MS.gene94743.t1 MTR_7g066410 62.651 83 31 0 339 421 431 513 1.12e-22 100
MS.gene94743.t1 MTR_7g066290 40.920 435 223 10 11 421 10 434 2.29e-88 275
MS.gene94743.t1 MTR_7g062280 37.910 488 210 12 16 421 10 486 9.73e-88 275
MS.gene94743.t1 MTR_7g066360 41.964 448 207 11 17 422 15 451 1.91e-87 274
MS.gene94743.t1 MTR_7g012680 39.869 459 199 8 16 422 14 447 2.33e-87 273
MS.gene94743.t1 MTR_3g007740 40.611 458 207 13 11 421 4 443 4.53e-86 270
MS.gene94743.t1 MTR_7g063880 37.838 481 208 10 16 427 11 469 1.68e-83 264
MS.gene94743.t1 MTR_1g041315 37.868 441 219 8 16 404 14 451 2.39e-81 259
MS.gene94743.t1 MTR_7g063660 36.842 475 220 11 16 421 9 472 6.50e-81 257
MS.gene94743.t1 MTR_7g063670 37.526 477 212 11 4 421 3 452 1.06e-79 254
MS.gene94743.t1 MTR_7g063920 37.229 462 191 9 16 420 11 430 1.40e-79 253
MS.gene94743.t1 MTR_7g066400 37.748 453 203 10 14 416 10 433 3.13e-77 247
MS.gene94743.t1 MTR_7g063830 39.035 456 216 14 11 421 1 439 5.49e-76 244
MS.gene94743.t1 MTR_7g066570 44.753 324 160 4 17 332 15 327 5.77e-76 247
MS.gene94743.t1 MTR_7g066570 58.333 72 30 0 351 422 411 482 1.61e-16 82.0
MS.gene94743.t1 MTR_7g058710 35.152 495 213 11 16 426 12 482 3.24e-74 240
MS.gene94743.t1 MTR_7g063900 42.229 341 180 5 12 344 5 336 7.52e-74 239
MS.gene94743.t1 MTR_7g058700 37.793 426 189 8 28 416 531 917 4.81e-73 246
MS.gene94743.t1 MTR_7g066310 36.281 441 185 11 16 422 7 385 5.04e-72 232
MS.gene94743.t1 MTR_7g063840 42.165 351 180 8 17 357 11 348 8.50e-70 228
MS.gene94743.t1 MTR_7g063890 41.783 359 170 9 12 350 21 360 1.07e-69 229
MS.gene94743.t1 MTR_7g063820 42.769 325 166 8 17 332 11 324 4.63e-65 216
MS.gene94743.t1 MTR_1g014060 36.881 404 219 6 27 422 11 386 3.16e-63 209
MS.gene94743.t1 MTR_7g066580 41.768 328 148 10 22 341 1 293 7.27e-61 204
MS.gene94743.t1 MTR_7g066580 55.556 63 28 0 351 413 376 438 4.56e-13 71.2
MS.gene94743.t1 MTR_4g019310 34.004 497 228 13 19 426 11 496 6.34e-60 203
MS.gene94743.t1 MTR_4g019340 32.150 479 222 13 17 408 39 501 1.77e-46 167
MS.gene94743.t1 MTR_4g017410 30.021 483 259 11 14 421 7 485 2.15e-45 164
MS.gene94743.t1 MTR_4g017610 29.769 477 233 13 19 413 16 472 4.71e-45 163
MS.gene94743.t1 MTR_2g029400 34.277 318 139 6 16 332 14 262 2.85e-43 157
MS.gene94743.t1 MTR_2g029400 26.190 168 107 3 263 413 15 182 1.03e-11 66.6
MS.gene94743.t1 MTR_2g029400 53.226 62 29 0 348 409 343 404 3.28e-11 65.1
MS.gene94743.t1 MTR_7g066280 30.118 425 167 14 95 422 1 392 4.29e-40 148
MS.gene94743.t1 MTR_3g065200 35.762 302 147 5 15 311 2 261 5.68e-40 149
MS.gene94743.t1 MTR_4g017470 33.824 340 177 6 17 344 13 316 5.15e-39 147
MS.gene94743.t1 MTR_4g017830 34.595 370 186 15 17 353 13 359 8.38e-39 147
MS.gene94743.t1 MTR_4g017830 38.614 101 51 1 320 420 412 501 4.02e-11 65.1
MS.gene94743.t1 MTR_4g017440 31.967 366 193 9 14 344 12 356 5.79e-35 135
MS.gene94743.t1 MTR_7g062270 42.262 168 91 2 17 178 11 178 1.20e-33 130
MS.gene94743.t1 MTR_7g063720 39.000 200 73 3 265 417 26 223 1.68e-33 126
MS.gene94743.t1 MTR_7g063720 28.261 230 140 10 6 229 10 220 7.24e-12 65.5
MS.gene94743.t1 MTR_7g063690 32.967 273 120 8 17 289 13 222 2.20e-28 112
MS.gene94743.t1 MTR_8g023240 29.560 318 191 10 17 323 38 333 6.41e-28 116
MS.gene94743.t1 MTR_4g017550 30.721 319 187 10 30 325 3 310 3.13e-27 113
MS.gene94743.t1 MTR_7g066680 31.090 312 114 8 212 429 37 341 3.70e-26 108
MS.gene94743.t1 MTR_7g066390 47.619 126 60 2 303 423 1 125 1.42e-22 94.0
MS.gene94743.t1 MTR_8g445860 31.557 244 143 8 7 235 4 238 7.76e-22 94.4
MS.gene94743.t1 MTR_1g115440 30.544 239 135 8 16 234 11 238 2.92e-18 84.0
MS.gene94743.t1 MTR_4g019330 37.349 166 89 3 257 412 13 173 1.71e-16 77.8
MS.gene94743.t1 MTR_0150s0010 28.333 240 147 5 12 234 6 237 1.54e-15 76.3
MS.gene94743.t1 MTR_2g067310 36.709 158 65 5 27 183 58 181 3.58e-15 75.1
MS.gene94743.t1 MTR_1g115450 44.318 88 44 1 12 99 3 85 1.41e-13 68.2
MS.gene94743.t1 MTR_7g063850 34.459 148 75 5 186 332 5 131 2.61e-13 70.5
MS.gene94743.t1 MTR_7g063850 39.326 89 54 0 341 429 203 291 3.33e-12 67.4
MS.gene94743.t1 MTR_1g115455 27.197 239 149 6 12 235 5 233 1.49e-12 67.4
MS.gene94743.t1 MTR_0150s0030 41.379 87 45 2 14 99 6 87 3.94e-12 65.5
MS.gene94743.t1 MTR_4g019240 29.825 171 79 3 262 420 13 154 6.46e-12 64.3
MS.gene94743.t1 MTR_7g063760 43.056 72 40 1 356 426 2 73 8.38e-12 60.8
MS.gene94743.t1 MTR_8g445910 40.449 89 47 2 12 99 4 87 4.93e-11 63.5
MS.gene94743.t1 MTR_8g044880 40.449 89 47 2 12 99 4 87 6.14e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene94743.t1 AT3G09140 32.525 495 248 12 14 425 7 498 1.88e-56 194
MS.gene94743.t1 AT3G09140 30.769 520 252 12 14 425 7 526 1.24e-53 187
MS.gene94743.t1 AT5G01120 31.006 487 238 12 17 416 10 485 8.88e-50 176
MS.gene94743.t1 AT5G01130 31.304 460 249 11 16 426 9 450 1.44e-49 174
MS.gene94743.t1 AT5G01140 28.361 476 245 8 18 426 11 457 9.83e-46 165
MS.gene94743.t1 AT3G09110 33.438 317 199 4 18 327 11 322 1.77e-45 161
MS.gene94743.t1 AT5G01150 28.810 479 257 11 18 421 11 480 1.34e-41 154
MS.gene94743.t1 AT5G37320 30.435 483 250 17 15 421 8 480 1.07e-40 151
MS.gene94743.t1 AT3G09120 32.407 324 188 7 12 316 2 313 4.67e-40 145
MS.gene94743.t1 AT5G43240 33.333 366 209 11 17 357 10 365 2.88e-37 142
MS.gene94743.t1 AT5G43240 33.333 366 209 11 17 357 10 365 2.88e-37 142
MS.gene94743.t1 AT5G43240 33.333 366 209 11 17 357 10 365 2.88e-37 142

Find 65 sgRNAs with CRISPR-Local

Find 101 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAAGTTTCTGTGAAAATTT+TGG 0.216022 7.1:-35423871 MS.gene94743:CDS
TATTGTTCCATCAAATTAAT+TGG 0.226939 7.1:+35423621 None:intergenic
TTGGATTTAAAATATCTTTC+AGG 0.240482 7.1:+35422538 None:intergenic
CAGTAGCAGCTTCGCTTCTT+AGG 0.241207 7.1:+35423516 None:intergenic
CTTAGACAATAAATCTGATT+TGG 0.246131 7.1:+35422519 None:intergenic
GAGAATTCCTCTCTCTGATT+TGG 0.271322 7.1:-35422374 MS.gene94743:CDS
TGAGAAATTTGAAAATTATC+AGG 0.280730 7.1:+35423387 None:intergenic
ATTCAAGAGCATGGTTGATT+TGG 0.281674 7.1:-35422563 MS.gene94743:CDS
TAAGAAAATTCTGTATGCTT+TGG 0.306163 7.1:-35422692 MS.gene94743:CDS
AAGAAAATTCTGTATGCTTT+GGG 0.312199 7.1:-35422691 MS.gene94743:CDS
AATGTATTGGATTTAGATTT+TGG 0.319002 7.1:-35422778 MS.gene94743:CDS
GGTTCGACCAATTAATTTGA+TGG 0.328737 7.1:-35423628 MS.gene94743:CDS
CAAGAGTCCTTCAACATATA+TGG 0.334427 7.1:-35422461 MS.gene94743:CDS
TTGTCATTGGTTGCAAGAAA+TGG 0.341523 7.1:+35422177 None:intergenic
GGAGTTGAGAACATGTTAAA+TGG 0.344732 7.1:-35422616 MS.gene94743:CDS
GTATCTTGGATGTGAAAATA+TGG 0.345574 7.1:-35423343 MS.gene94743:CDS
TCCAATACATTGACAGGTCT+TGG 0.352633 7.1:+35422790 None:intergenic
GTTTCTCTTCTGAAGTATCT+TGG 0.357240 7.1:-35423357 MS.gene94743:CDS
AGTTTCTTAACTTTGCCATT+AGG 0.370562 7.1:-35423783 MS.gene94743:CDS
AAAATTCTCTTTGTCAAGGT+TGG 0.380539 7.1:+35423677 None:intergenic
ATTTAGTGAAGTGCTCCTTA+TGG 0.380971 7.1:-35422867 MS.gene94743:CDS
GCATGGGAGCACACCTTGTA+AGG 0.388782 7.1:-35422753 MS.gene94743:CDS
CTTCTATTGTTCCCCTTACA+AGG 0.392190 7.1:+35422740 None:intergenic
GACAAGAATACGTCTGTTAG+AGG 0.400508 7.1:+35422835 None:intergenic
TCATCATCAGCTCTAACTAT+TGG 0.402695 7.1:-35422207 MS.gene94743:CDS
ACCAAGACCTGTCAATGTAT+TGG 0.433905 7.1:-35422791 MS.gene94743:CDS
CAACAAAGTCCTTCCCTGCT+TGG 0.437327 7.1:+35423817 None:intergenic
CACAGAAACTTCAGTGGATG+GGG 0.462605 7.1:+35423881 None:intergenic
GAAGAAAGAATGATTAGCAT+TGG 0.467741 7.1:-35422352 MS.gene94743:CDS
TCTGTTACCATATATGTTGA+AGG 0.472437 7.1:+35422454 None:intergenic
TCTAAATCCAATACATTGAC+AGG 0.482402 7.1:+35422784 None:intergenic
TCAGCTCTAACTATTGGTCT+TGG 0.483988 7.1:-35422201 MS.gene94743:CDS
ACCTAAAATTCTCTTTGTCA+AGG 0.486256 7.1:+35423673 None:intergenic
TTCACAGAAACTTCAGTGGA+TGG 0.488867 7.1:+35423879 None:intergenic
GGAACAAAACCATTCTTAGC+AGG 0.489021 7.1:+35423453 None:intergenic
TAGAGGTGTCGCCGACCATA+AGG 0.500207 7.1:+35422852 None:intergenic
AGTCTTGCAATAGTGCCTAA+TGG 0.507578 7.1:+35423768 None:intergenic
ATAGATTATCTATGTTGTCT+AGG 0.508322 7.1:+35422584 None:intergenic
AAAGGAAATCGCATCGGTAG+AGG 0.509174 7.1:+35422408 None:intergenic
TCACAGAAACTTCAGTGGAT+GGG 0.511080 7.1:+35423880 None:intergenic
AAGGGGAACAATAGAAGTAA+AGG 0.522163 7.1:-35422734 MS.gene94743:CDS
AACATGTTAAATGGAAACTC+TGG 0.523592 7.1:-35422607 MS.gene94743:CDS
ATAAGAAAAGTTGCGGTCAC+CGG 0.523846 7.1:+35423432 None:intergenic
TCTGTATGCTTTGGGTGAAG+AGG 0.540891 7.1:-35422683 MS.gene94743:CDS
GACTGTTGATATCACTCGTA+AGG 0.541024 7.1:-35423304 MS.gene94743:CDS
CTTTCTTCCAAATCAGAGAG+AGG 0.543946 7.1:+35422367 None:intergenic
AACATGAAGAATGTTAGTGT+TGG 0.544227 7.1:-35423729 MS.gene94743:CDS
TGTTCTCAACTCCTCCCAAA+GGG 0.549781 7.1:+35422626 None:intergenic
GATTAGCATTGGCAATAAAG+AGG 0.572173 7.1:-35422341 MS.gene94743:intron
CATGGGAGCACACCTTGTAA+GGG 0.584012 7.1:-35422752 MS.gene94743:CDS
ATCTGAGATAAGAAAAGTTG+CGG 0.589869 7.1:+35423425 None:intergenic
AGATAATCTATTCAAGAGCA+TGG 0.593603 7.1:-35422572 MS.gene94743:CDS
ATCGCATCGGTAGAGGAACC+TGG 0.597504 7.1:+35422415 None:intergenic
TTTATTTGTCCAAGCAGGGA+AGG 0.601075 7.1:-35423826 MS.gene94743:CDS
AGTGAAGTGCTCCTTATGGT+CGG 0.606083 7.1:-35422863 MS.gene94743:CDS
AACAATAGAAGTAAAGGCAA+TGG 0.611013 7.1:-35422728 MS.gene94743:CDS
GTTACGTAGGAAAGTAGCCA+TGG 0.616515 7.1:+35423917 None:intergenic
ATGTTCTCAACTCCTCCCAA+AGG 0.619718 7.1:+35422625 None:intergenic
GAAAAGAACCTATTTATGTG+AGG 0.621159 7.1:-35423484 MS.gene94743:CDS
AGTCAGAAAGGAAATCGCAT+CGG 0.623415 7.1:+35422402 None:intergenic
ATGGGAGCACACCTTGTAAG+GGG 0.628802 7.1:-35422751 MS.gene94743:CDS
TTGATGACACAGAAAAGTTG+AGG 0.631875 7.1:-35423575 MS.gene94743:CDS
CAAAGCAATTGAAGTTACGT+AGG 0.644804 7.1:+35423904 None:intergenic
TGTTGATATCACTCGTAAGG+AGG 0.661482 7.1:-35423301 MS.gene94743:intron
GATGACTTAGTCATCACACC+AGG 0.706265 7.1:-35422433 MS.gene94743:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AAATTTTGTGATTGTATTTA+TGG + chr7.1:35423113-35423132 None:intergenic 15.0%
!!! AGATTTTGAAAAAGAATTAT+TGG - chr7.1:35422427-35422446 MS.gene94743:CDS 15.0%
!!! ATACATTTTAGAATTTACAA+AGG + chr7.1:35423157-35423176 None:intergenic 15.0%
!!! CTTATGTTTTAATTTTTTTG+TGG - chr7.1:35423808-35423827 MS.gene94743:CDS 15.0%
!! GTTATAGACATATAACAATT+TGG - chr7.1:35422984-35423003 MS.gene94743:intron 20.0%
!! TATTGTTCCATCAAATTAAT+TGG + chr7.1:35422458-35422477 None:intergenic 20.0%
!! TGAGAAATTTGAAAATTATC+AGG + chr7.1:35422692-35422711 None:intergenic 20.0%
!!! AATGTATTGGATTTAGATTT+TGG - chr7.1:35423298-35423317 MS.gene94743:intron 20.0%
!!! TTGGATTTAAAATATCTTTC+AGG + chr7.1:35423541-35423560 None:intergenic 20.0%
!!! TTTTTTAACCTCACATAAAT+AGG + chr7.1:35422603-35422622 None:intergenic 20.0%
! AAAAAGAAGTTAATTCCCAA+AGG - chr7.1:35422909-35422928 MS.gene94743:intron 25.0%
! AAGAAAATTCTGTATGCTTT+GGG - chr7.1:35423385-35423404 MS.gene94743:CDS 25.0%
! ATAGATTATCTATGTTGTCT+AGG + chr7.1:35423495-35423514 None:intergenic 25.0%
! CATTTATATTGTAATCTCCT+AGG + chr7.1:35422963-35422982 None:intergenic 25.0%
! CTTAGACAATAAATCTGATT+TGG + chr7.1:35423560-35423579 None:intergenic 25.0%
! TAAGAAAATTCTGTATGCTT+TGG - chr7.1:35423384-35423403 MS.gene94743:CDS 25.0%
!! AGCATACAGAATTTTCTTAT+TGG + chr7.1:35423383-35423402 None:intergenic 25.0%
!! CAAGTAAAATCCAATTTTGA+TGG - chr7.1:35423580-35423599 MS.gene94743:CDS 25.0%
!! TGAAGTTTCTGTGAAAATTT+TGG - chr7.1:35422205-35422224 MS.gene94743:CDS 25.0%
!! TGGGAATTAACTTCTTTTTA+CGG + chr7.1:35422908-35422927 None:intergenic 25.0%
!!! AAATTTTTTGACATTCCCTT+TGG - chr7.1:35423435-35423454 MS.gene94743:CDS 25.0%
!!! AATTCTCATTTTAGTCAGAA+AGG + chr7.1:35423689-35423708 None:intergenic 25.0%
!!! AATTTTTTGACATTCCCTTT+GGG - chr7.1:35423436-35423455 MS.gene94743:CDS 25.0%
!!! GATTTGTTTTCCATCAAAAT+TGG + chr7.1:35423593-35423612 None:intergenic 25.0%
!!! GTTTAAGATTTTCTTGTCAT+TGG + chr7.1:35423915-35423934 None:intergenic 25.0%
AAAATTCTCTTTGTCAAGGT+TGG + chr7.1:35422402-35422421 None:intergenic 30.0%
AACAATAGAAGTAAAGGCAA+TGG - chr7.1:35423348-35423367 MS.gene94743:CDS 30.0%
AACATGAAGAATGTTAGTGT+TGG - chr7.1:35422347-35422366 MS.gene94743:CDS 30.0%
AACATGTTAAATGGAAACTC+TGG - chr7.1:35423469-35423488 MS.gene94743:CDS 30.0%
ACAATTCCTATGATTTCCTT+TGG + chr7.1:35422928-35422947 None:intergenic 30.0%
ACCTAAAATTCTCTTTGTCA+AGG + chr7.1:35422406-35422425 None:intergenic 30.0%
AGATAATCTATTCAAGAGCA+TGG - chr7.1:35423504-35423523 MS.gene94743:CDS 30.0%
AGTTTCTTAACTTTGCCATT+AGG - chr7.1:35422293-35422312 MS.gene94743:intron 30.0%
CAATTCCTATGATTTCCTTT+GGG + chr7.1:35422927-35422946 None:intergenic 30.0%
CATAAGAAACATGAACTTGT+CGG + chr7.1:35423794-35423813 None:intergenic 30.0%
GAAAAGAACCTATTTATGTG+AGG - chr7.1:35422592-35422611 MS.gene94743:CDS 30.0%
GAAGAAAGAATGATTAGCAT+TGG - chr7.1:35423724-35423743 MS.gene94743:CDS 30.0%
GTATCTTGGATGTGAAAATA+TGG - chr7.1:35422733-35422752 MS.gene94743:CDS 30.0%
GTTAAAAAACCTGCTAAGAA+TGG - chr7.1:35422614-35422633 MS.gene94743:CDS 30.0%
TAATTCCCAAAGGAAATCAT+AGG - chr7.1:35422919-35422938 MS.gene94743:intron 30.0%
TCTAAATCCAATACATTGAC+AGG + chr7.1:35423295-35423314 None:intergenic 30.0%
TCTGTTACCATATATGTTGA+AGG + chr7.1:35423625-35423644 None:intergenic 30.0%
! AATTTTCACAGAAACTTCAG+TGG + chr7.1:35422204-35422223 None:intergenic 30.0%
! ACCTTGACAAAGAGAATTTT+AGG - chr7.1:35422402-35422421 MS.gene94743:CDS 30.0%
! ATCTGAGATAAGAAAAGTTG+CGG + chr7.1:35422654-35422673 None:intergenic 30.0%
!! AAGTTGAGGTCTTTTAATTG+TGG - chr7.1:35422515-35422534 MS.gene94743:CDS 30.0%
!! GATTTAGATTTTGGTAGCAT+GGG - chr7.1:35423307-35423326 MS.gene94743:CDS 30.0%
!!! AAAGTTTTATTTGTCCAAGC+AGG - chr7.1:35422245-35422264 MS.gene94743:intron 30.0%
!!! AAGTTTTATTTGTCCAAGCA+GGG - chr7.1:35422246-35422265 MS.gene94743:intron 30.0%
!!! TAATTTTTTTGTGGAACTGC+AGG - chr7.1:35423817-35423836 MS.gene94743:CDS 30.0%
AAGGGGAACAATAGAAGTAA+AGG - chr7.1:35423342-35423361 MS.gene94743:CDS 35.0%
CAAAGCAATTGAAGTTACGT+AGG + chr7.1:35422175-35422194 None:intergenic 35.0%
CAAGAGTCCTTCAACATATA+TGG - chr7.1:35423615-35423634 MS.gene94743:CDS 35.0%
GGAGTTGAGAACATGTTAAA+TGG - chr7.1:35423460-35423479 MS.gene94743:CDS 35.0%
! ATTCAAGAGCATGGTTGATT+TGG - chr7.1:35423513-35423532 MS.gene94743:CDS 35.0%
! ATTTAGTGAAGTGCTCCTTA+TGG - chr7.1:35423209-35423228 MS.gene94743:intron 35.0%
! GGTTCGACCAATTAATTTGA+TGG - chr7.1:35422448-35422467 MS.gene94743:CDS 35.0%
! TCATCATCAGCTCTAACTAT+TGG - chr7.1:35423869-35423888 MS.gene94743:CDS 35.0%
! TGTAATCTCCTAGGATTAGT+AGG + chr7.1:35422954-35422973 None:intergenic 35.0%
! TTGATGACACAGAAAAGTTG+AGG - chr7.1:35422501-35422520 MS.gene94743:CDS 35.0%
! TTGTCATTGGTTGCAAGAAA+TGG + chr7.1:35423902-35423921 None:intergenic 35.0%
!! GATTAGCATTGGCAATAAAG+AGG - chr7.1:35423735-35423754 MS.gene94743:CDS 35.0%
!! GGATTTAGATTTTGGTAGCA+TGG - chr7.1:35423306-35423325 MS.gene94743:CDS 35.0%
!! GTTTCTCTTCTGAAGTATCT+TGG - chr7.1:35422719-35422738 MS.gene94743:CDS 35.0%
!!! TGCTAAGAATGGTTTTGTTC+CGG - chr7.1:35422625-35422644 MS.gene94743:CDS 35.0%
ACCAAGACCTGTCAATGTAT+TGG - chr7.1:35423285-35423304 MS.gene94743:intron 40.0%
ACTAGTATGCCTGAACTTCT+TGG + chr7.1:35423082-35423101 None:intergenic 40.0%
AGTCAGAAAGGAAATCGCAT+CGG + chr7.1:35423677-35423696 None:intergenic 40.0%
AGTCTTGCAATAGTGCCTAA+TGG + chr7.1:35422311-35422330 None:intergenic 40.0%
ATAAGAAAAGTTGCGGTCAC+CGG + chr7.1:35422647-35422666 None:intergenic 40.0%
ATTGTCCACCTACTAATCCT+AGG - chr7.1:35422943-35422962 MS.gene94743:intron 40.0%
CATGATTTCCCAAGAAGTTC+AGG - chr7.1:35423070-35423089 MS.gene94743:intron 40.0%
CTAGTATGCCTGAACTTCTT+GGG + chr7.1:35423081-35423100 None:intergenic 40.0%
CTTCTATTGTTCCCCTTACA+AGG + chr7.1:35423339-35423358 None:intergenic 40.0%
CTTTCTTCCAAATCAGAGAG+AGG + chr7.1:35423712-35423731 None:intergenic 40.0%
GACAAGAATACGTCTGTTAG+AGG + chr7.1:35423244-35423263 None:intergenic 40.0%
GACTGTTGATATCACTCGTA+AGG - chr7.1:35422772-35422791 MS.gene94743:CDS 40.0%
GAGAATTCCTCTCTCTGATT+TGG - chr7.1:35423702-35423721 MS.gene94743:CDS 40.0%
GGAACAAAACCATTCTTAGC+AGG + chr7.1:35422626-35422645 None:intergenic 40.0%
TCACAGAAACTTCAGTGGAT+GGG + chr7.1:35422199-35422218 None:intergenic 40.0%
TCCAATACATTGACAGGTCT+TGG + chr7.1:35423289-35423308 None:intergenic 40.0%
TGTTGATATCACTCGTAAGG+AGG - chr7.1:35422775-35422794 MS.gene94743:CDS 40.0%
TTCACAGAAACTTCAGTGGA+TGG + chr7.1:35422200-35422219 None:intergenic 40.0%
TTTATTTGTCCAAGCAGGGA+AGG - chr7.1:35422250-35422269 MS.gene94743:intron 40.0%
! AATCTCCTAGGATTAGTAGG+TGG + chr7.1:35422951-35422970 None:intergenic 40.0%
! TCAGCTCTAACTATTGGTCT+TGG - chr7.1:35423875-35423894 MS.gene94743:CDS 40.0%
! TTTTTGACATTCCCTTTGGG+AGG - chr7.1:35423439-35423458 MS.gene94743:CDS 40.0%
ATGTTCTCAACTCCTCCCAA+AGG + chr7.1:35423454-35423473 None:intergenic 45.0%
CACAGAAACTTCAGTGGATG+GGG + chr7.1:35422198-35422217 None:intergenic 45.0%
GATGACTTAGTCATCACACC+AGG - chr7.1:35423643-35423662 MS.gene94743:CDS 45.0%
TGTTCTCAACTCCTCCCAAA+GGG + chr7.1:35423453-35423472 None:intergenic 45.0%
! AAAGGAAATCGCATCGGTAG+AGG + chr7.1:35423671-35423690 None:intergenic 45.0%
! AGTGAAGTGCTCCTTATGGT+CGG - chr7.1:35423213-35423232 MS.gene94743:intron 45.0%
! TCTGTATGCTTTGGGTGAAG+AGG - chr7.1:35423393-35423412 MS.gene94743:CDS 45.0%
CAACAAAGTCCTTCCCTGCT+TGG + chr7.1:35422262-35422281 None:intergenic 50.0%
CAGTAGCAGCTTCGCTTCTT+AGG + chr7.1:35422563-35422582 None:intergenic 50.0%
! ATGGGAGCACACCTTGTAAG+GGG - chr7.1:35423325-35423344 MS.gene94743:CDS 50.0%
! CATGGGAGCACACCTTGTAA+GGG - chr7.1:35423324-35423343 MS.gene94743:CDS 50.0%
! ATCGCATCGGTAGAGGAACC+TGG + chr7.1:35423664-35423683 None:intergenic 55.0%
! GCATGGGAGCACACCTTGTA+AGG - chr7.1:35423323-35423342 MS.gene94743:CDS 55.0%
!! TAGAGGTGTCGCCGACCATA+AGG + chr7.1:35423227-35423246 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr7.1 gene 35422161 35423937 35422161 ID=MS.gene94743
chr7.1 mRNA 35422161 35423937 35422161 ID=MS.gene94743.t1;Parent=MS.gene94743
chr7.1 exon 35423302 35423937 35423302 ID=MS.gene94743.t1.exon1;Parent=MS.gene94743.t1
chr7.1 CDS 35423302 35423937 35423302 ID=cds.MS.gene94743.t1;Parent=MS.gene94743.t1
chr7.1 exon 35422342 35422896 35422342 ID=MS.gene94743.t1.exon2;Parent=MS.gene94743.t1
chr7.1 CDS 35422342 35422896 35422342 ID=cds.MS.gene94743.t1;Parent=MS.gene94743.t1
chr7.1 exon 35422161 35422259 35422161 ID=MS.gene94743.t1.exon3;Parent=MS.gene94743.t1
chr7.1 CDS 35422161 35422259 35422161 ID=cds.MS.gene94743.t1;Parent=MS.gene94743.t1
Gene Sequence

>MS.gene94743

ATGGCTACTTTCCTACGTAACTTCAATTGCTTTGCCCCATCCACTGAAGTTTCTGTGAAAATTTTGGTAGATGAAGTCAGAAACAAAGTTTTATTTGTCCAAGCAGGGAAGGACTTTGTTGATGTGCTGCTAAGTTTCTTAACTTTGCCATTAGGCACTATTGCAAGACTTTTGAGTCAAGAATCTAACATGAAGAATGTTAGTGTTGGAAGCTTAAGCTTGTTATATCAAAGTGTTTCCAACCTTGACAAAGAGAATTTTAGGTCAGATTTTGAAAAAGAATTATTGGTTCGACCAATTAATTTGATGGAACAATATTGCAAGTATCTAAAACTCAACATTGATGACACAGAAAAGTTGAGGTCTTTTAATTGTGGCAAACAAAAGTGCATTGATATACCTAAGAAGCGAAGCTGCTACTGCTGTGAAAAGAAAAGAACCTATTTATGTGAGGTTAAAAAACCTGCTAAGAATGGTTTTGTTCCGGTGACCGCAACTTTTCTTATCTCAGATGATTTGAATGTGAAGCCTGATAATTTTCAAATTTCTCAAAATAGTGTTTCTCTTCTGAAGTATCTTGGATGTGAAAATATGGATTCTATCAAGATAGTGACTGTTGATATCACTCGTAAGGAGGTATCTATCTCTTTTGAAAATTCTCAATACTCTTGTATAATTTATGTCTAATGTGATTTTCTTTGCTTTTAAATTTGCTAAAAAGACTTTATGAATATAGACGAAAAACCGTAAAAAGAAGTTAATTCCCAAAGGAAATCATAGGAATTGTCCACCTACTAATCCTAGGAGATTACAATATAAATGTGTTATAGACATATAACAATTTGGTAGAAATATGCTGTAATTTGTATCTACTTTCATGTTGCATATATTGAAAGTTATTGAATTTTACATGATTTCCCAAGAAGTTCAGGCATACTAGTACTATCATCCATAAATACAATCACAAAATTTTGTTCAAATTTATCTAAATTTCCTTTGTAAATTCTAAAATGTATAACATTGTACATTGCTTTATTGTAGATACTAGATTTAGTGAAGTGCTCCTTATGGTCGGCGACACCTCTAACAGACGTATTCTTGTCGAAGAAACTGTTTATTGAGAAACCAAGACCTGTCAATGTATTGGATTTAGATTTTGGTAGCATGGGAGCACACCTTGTAAGGGGAACAATAGAAGTAAAGGCAATGGTGAGAAAATCCAATAAGAAAATTCTGTATGCTTTGGGTGAAGAGGACTTTGCAGACTTGTTACTAAATTTTTTGACATTCCCTTTGGGAGGAGTTGAGAACATGTTAAATGGAAACTCTGGCCTAGACAACATAGATAATCTATTCAAGAGCATGGTTGATTTGGATCCTGAAAGATATTTTAAATCCAAATCAGATTTATTGTCTAAGCAAGTAAAATCCAATTTTGATGGAAAACAAATCATCAAGAGTCCTTCAACATATATGGTAACAGATGACTTAGTCATCACACCAGGTTCCTCTACCGATGCGATTTCCTTTCTGACTAAAATGAGAATTCCTCTCTCTGATTTGGAAGAAAGAATGATTAGCATTGGCAATAAAGAGGTAAATTGCTGTTGTCGATTTCATTTCTTTTATTCCGACAAGTTCATGTTTCTTATGTTTTAATTTTTTTGTGGAACTGCAGGCTCATAGTCTTCTTAAAGCTTCTTTGATCTCATCATCAGCTCTAACTATTGGTCTTGGTCCATTTCTTGCAACCAATGACAAGAAAATCTTAAACTAA
Protein sequence

>MS.gene94743.t1

MATFLRNFNCFAPSTEVSVKILVDEVRNKVLFVQAGKDFVDVLLSFLTLPLGTIARLLSQESNMKNVSVGSLSLLYQSVSNLDKENFRSDFEKELLVRPINLMEQYCKYLKLNIDDTEKLRSFNCGKQKCIDIPKKRSCYCCEKKRTYLCEVKKPAKNGFVPVTATFLISDDLNVKPDNFQISQNSVSLLKYLGCENMDSIKIVTVDITRKEILDLVKCSLWSATPLTDVFLSKKLFIEKPRPVNVLDLDFGSMGAHLVRGTIEVKAMVRKSNKKILYALGEEDFADLLLNFLTFPLGGVENMLNGNSGLDNIDNLFKSMVDLDPERYFKSKSDLLSKQVKSNFDGKQIIKSPSTYMVTDDLVITPGSSTDAISFLTKMRIPLSDLEERMISIGNKEAHSLLKASLISSSALTIGLGPFLATNDKKILN