AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70046

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70046.t1 MTR_4g017440 82.168 286 34 2 1 286 240 508 1.33e-157 449
MS.gene70046.t1 MTR_4g017470 63.066 287 95 3 1 286 200 476 1.17e-112 334
MS.gene70046.t1 MTR_4g017470 36.937 111 53 2 38 131 15 125 6.42e-13 68.9
MS.gene70046.t1 MTR_4g017830 61.592 289 93 4 1 286 234 507 1.12e-110 330
MS.gene70046.t1 MTR_4g019340 60.148 271 70 4 1 270 270 503 1.20e-93 286
MS.gene70046.t1 MTR_4g017410 57.840 287 85 5 1 286 239 490 1.36e-93 285
MS.gene70046.t1 MTR_4g017410 27.027 259 138 9 38 274 12 241 8.91e-15 74.3
MS.gene70046.t1 MTR_4g017550 59.431 281 87 7 1 276 211 469 2.06e-93 284
MS.gene70046.t1 MTR_4g017610 48.057 283 98 6 1 277 237 476 2.73e-68 219
MS.gene70046.t1 MTR_4g017610 27.843 255 139 8 38 276 16 241 9.04e-12 65.5
MS.gene70046.t1 MTR_4g019310 39.051 274 133 6 20 286 251 497 1.94e-47 165
MS.gene70046.t1 MTR_4g019310 28.627 255 142 8 35 273 8 238 1.28e-12 68.2
MS.gene70046.t1 MTR_7g066430 34.241 257 134 7 33 280 255 485 7.34e-28 112
MS.gene70046.t1 MTR_7g066280 34.000 250 146 7 38 280 153 390 2.31e-27 110
MS.gene70046.t1 MTR_7g066570 33.068 251 136 4 35 280 257 480 1.93e-26 108
MS.gene70046.t1 MTR_7g058710 30.566 265 153 6 27 286 245 483 5.31e-25 104
MS.gene70046.t1 MTR_7g058710 25.896 251 143 8 30 273 7 221 3.23e-14 72.8
MS.gene70046.t1 MTR_7g066310 32.099 243 110 5 35 277 193 380 7.00e-24 100
MS.gene70046.t1 MTR_7g066310 31.783 129 77 3 35 152 7 135 2.58e-12 67.0
MS.gene70046.t1 MTR_7g066400 34.252 254 112 7 27 277 233 434 8.60e-24 100
MS.gene70046.t1 MTR_7g066340 30.924 249 111 4 35 280 263 453 2.44e-23 99.4
MS.gene70046.t1 MTR_7g066340 29.317 249 137 9 35 273 12 231 5.41e-16 78.2
MS.gene70046.t1 MTR_7g066580 32.806 253 137 9 35 280 215 441 2.86e-23 99.0
MS.gene70046.t1 MTR_7g066580 39.785 93 49 3 46 131 6 98 2.12e-11 64.3
MS.gene70046.t1 MTR_7g066350 32.283 254 109 6 27 277 253 446 1.24e-22 97.4
MS.gene70046.t1 MTR_7g066350 27.419 248 143 8 35 274 9 227 1.83e-13 70.5
MS.gene70046.t1 MTR_7g066410 30.980 255 149 4 31 280 280 512 4.27e-22 95.9
MS.gene70046.t1 MTR_8g445860 28.340 247 151 5 31 274 9 232 2.00e-21 90.9
MS.gene70046.t1 MTR_7g066680 33.624 229 124 6 33 259 84 286 3.54e-21 92.0
MS.gene70046.t1 MTR_1g041315 30.741 270 122 9 1 264 241 451 5.57e-21 92.8
MS.gene70046.t1 MTR_7g066260 32.245 245 114 6 32 276 266 458 1.87e-20 91.3
MS.gene70046.t1 MTR_7g066260 29.600 250 139 11 33 273 13 234 6.17e-14 72.0
MS.gene70046.t1 MTR_7g012680 31.373 255 126 5 35 286 243 451 2.53e-20 90.5
MS.gene70046.t1 MTR_7g012680 34.711 121 62 5 35 148 14 124 8.41e-12 65.5
MS.gene70046.t1 MTR_7g062300 29.804 255 139 6 31 279 252 472 5.84e-20 89.7
MS.gene70046.t1 MTR_7g062300 28.740 254 140 9 30 273 7 229 2.44e-15 76.3
MS.gene70046.t1 MTR_7g063900 30.827 266 161 7 19 280 236 482 2.62e-18 84.7
MS.gene70046.t1 MTR_7g063900 35.577 104 60 2 35 131 10 113 2.75e-12 67.0
MS.gene70046.t1 MTR_7g063890 32.365 241 138 8 45 280 278 498 5.38e-17 81.3
MS.gene70046.t1 MTR_7g063890 28.689 244 134 9 38 273 30 241 9.90e-12 65.5
MS.gene70046.t1 MTR_7g063660 29.600 250 145 8 40 286 256 477 1.08e-16 80.1
MS.gene70046.t1 MTR_7g063660 40.351 114 60 4 35 141 9 121 2.21e-16 79.3
MS.gene70046.t1 MTR_1g115455 29.134 254 141 9 30 274 4 227 7.24e-16 75.5
MS.gene70046.t1 MTR_4g019350 40.187 107 41 1 93 199 1 84 7.18e-15 68.9
MS.gene70046.t1 MTR_0150s0010 27.092 251 154 7 30 274 5 232 7.31e-15 72.8
MS.gene70046.t1 MTR_7g063880 36.364 132 74 5 30 154 6 134 8.37e-15 74.3
MS.gene70046.t1 MTR_0150s0030 35.849 106 59 3 30 132 3 102 8.70e-15 72.0
MS.gene70046.t1 MTR_8g445910 35.833 120 66 4 30 144 3 116 1.23e-14 72.8
MS.gene70046.t1 MTR_1g115450 41.176 85 48 1 31 113 3 87 1.29e-14 69.7
MS.gene70046.t1 MTR_7g058700 45.977 87 44 1 35 118 752 838 1.69e-14 73.9
MS.gene70046.t1 MTR_7g063830 28.216 241 131 10 48 278 23 231 2.91e-14 72.8
MS.gene70046.t1 MTR_1g014060 28.740 254 123 7 38 286 190 390 4.62e-14 72.0
MS.gene70046.t1 MTR_7g062280 30.085 236 136 10 48 273 23 239 1.43e-13 70.9
MS.gene70046.t1 MTR_8g044880 35.833 120 66 4 30 144 3 116 1.45e-13 69.7
MS.gene70046.t1 MTR_7g063820 38.318 107 59 3 36 136 11 116 1.61e-13 70.9
MS.gene70046.t1 MTR_7g066290 36.434 129 79 1 1 126 233 361 1.62e-13 70.5
MS.gene70046.t1 MTR_4g019240 67.347 49 16 0 238 286 112 160 3.94e-13 67.0
MS.gene70046.t1 MTR_1g115440 26.506 249 158 5 30 274 6 233 6.06e-13 67.4
MS.gene70046.t1 MTR_4g019330 41.304 92 52 2 197 286 97 188 3.96e-12 64.3
MS.gene70046.t1 MTR_7g100420 28.986 276 156 9 14 281 155 398 5.44e-12 65.9
MS.gene70046.t1 MTR_7g063840 34.746 118 69 3 36 146 11 127 6.47e-12 65.9
MS.gene70046.t1 MTR_2g029400 37.500 104 58 4 35 131 14 117 1.15e-11 65.1
MS.gene70046.t1 MTR_7g066490 35.043 117 69 3 29 138 10 126 1.31e-11 64.7
MS.gene70046.t1 MTR_7g063920 33.884 121 73 2 30 143 6 126 3.49e-11 63.5
MS.gene70046.t1 MTR_8g445920 38.806 67 41 0 30 96 5 71 4.34e-11 59.3
MS.gene70046.t1 MTR_8g044890 38.806 67 41 0 30 96 5 71 4.34e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70046.t1 AT5G01140 33.217 286 137 14 16 286 212 458 8.64e-21 92.0
MS.gene70046.t1 AT5G01120 28.966 290 157 10 1 276 231 485 3.05e-20 90.5
MS.gene70046.t1 AT5G01150 29.707 239 127 7 48 280 276 479 3.16e-17 81.6
MS.gene70046.t1 AT3G09140 31.356 236 126 10 48 280 291 493 6.21e-17 80.9
MS.gene70046.t1 AT3G09140 31.356 236 126 10 48 280 319 521 7.60e-17 80.9
MS.gene70046.t1 AT5G43240 27.304 293 159 10 1 276 90 345 3.18e-16 78.2
MS.gene70046.t1 AT5G43240 27.304 293 159 10 1 276 234 489 6.31e-16 78.2
MS.gene70046.t1 AT5G43240 26.800 250 147 8 36 273 10 235 3.94e-12 66.6
MS.gene70046.t1 AT5G43240 27.304 293 159 10 1 276 234 489 6.31e-16 78.2
MS.gene70046.t1 AT5G43240 26.800 250 147 8 36 273 10 235 3.94e-12 66.6
MS.gene70046.t1 AT5G43240 27.304 293 159 10 1 276 234 489 6.31e-16 78.2
MS.gene70046.t1 AT5G43240 26.800 250 147 8 36 273 10 235 3.94e-12 66.6
MS.gene70046.t1 AT3G09110 25.806 248 144 5 35 273 9 225 5.95e-13 68.6
MS.gene70046.t1 AT5G37320 28.030 132 87 2 31 154 5 136 7.93e-11 62.8

Find 75 sgRNAs with CRISPR-Local

Find 87 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGACATGTTCGGAGATTT+TGG 0.150642 4.2:+84668692 None:intergenic
CCCACTTGTCAAGGTAAATT+TGG 0.181828 4.2:+84668389 None:intergenic
CCTGGCGCAGCACCACATTT+TGG 0.210743 4.2:-84668947 MS.gene70046:CDS
ACCATCTGAAAGCTTTAAAA+TGG 0.212595 4.2:+84669063 None:intergenic
TTAATTCTTTCAAATATAAA+AGG 0.238698 4.2:+84668582 None:intergenic
GGTACTGGTGTACCAAATTT+TGG 0.258982 4.2:-84668866 MS.gene70046:CDS
CACAAGTGGCGGCACTAAAA+TGG 0.259934 4.2:-84669191 MS.gene70046:CDS
TGGTTTGGACAACCAAAATT+TGG 0.263143 4.2:+84668854 None:intergenic
AATCAGAAATGTTGAGGAAT+TGG 0.275365 4.2:+84668834 None:intergenic
CTGCCCACTACATATTATTA+TGG 0.306674 4.2:-84668806 MS.gene70046:CDS
AAGTGGGCTGGGAAACCTTT+TGG 0.309378 4.2:-84668373 MS.gene70046:CDS
CCGCCCACTACATATTATTA+TGG 0.322722 4.2:-84668887 MS.gene70046:CDS
AGGCAGGTTGCCTTTGTACT+TGG 0.329588 4.2:+84669249 None:intergenic
AGCTTCCTTACCATGCCATT+AGG 0.332201 4.2:-84669088 MS.gene70046:CDS
TTCAAAATTGTATTTGTCAT+AGG 0.339563 4.2:+84669280 None:intergenic
GCTTCCTTACCATGCCATTA+GGG 0.353748 4.2:-84669087 MS.gene70046:CDS
GTGCAGAGATTAGCATTATA+CGG 0.353949 4.2:-84668650 MS.gene70046:CDS
CTCTAACGGCTGATGAAGAT+TGG 0.361391 4.2:+84669224 None:intergenic
TCAGATGGTAACTCGTTTGC+TGG 0.365227 4.2:-84669049 MS.gene70046:CDS
GGAATGTTGAGGGGCTGGTT+TGG 0.384748 4.2:+84668920 None:intergenic
GAAATGTTGAGGAATTGGTT+TGG 0.428433 4.2:+84668839 None:intergenic
CCAAAATGTGGTGCTGCGCC+AGG 0.440390 4.2:+84668947 None:intergenic
CTCCACTTGAAGATCTTCAA+TGG 0.442005 4.2:+84668553 None:intergenic
TCCAAATTTACCTTGACAAG+TGG 0.443877 4.2:-84668390 MS.gene70046:CDS
CCACCTTCCCTTGACATGTT+CGG 0.449279 4.2:+84668683 None:intergenic
CGCACTGTGTTACTCAATCC+TGG 0.461909 4.2:-84668965 MS.gene70046:CDS
CGGCACTAAAATGGATGTTA+AGG 0.464791 4.2:-84669182 MS.gene70046:CDS
GTGTAGGCTCTGAGCATCCT+TGG 0.469524 4.2:-84668426 MS.gene70046:intron
GTACAAGAGTGTGGAGAACT+TGG 0.471755 4.2:-84669011 MS.gene70046:CDS
TTTACCTTGACAAGTGGGCT+GGG 0.472395 4.2:-84668384 MS.gene70046:CDS
ATTTACCTTGACAAGTGGGC+TGG 0.473382 4.2:-84668385 MS.gene70046:CDS
ACTACATATTATTATGGTAC+TGG 0.476342 4.2:-84668881 MS.gene70046:CDS
CCTTCCTTGATATATTTATG+TGG 0.478610 4.2:+84668773 None:intergenic
AATCAGGAATGTTGAGGGGC+TGG 0.483938 4.2:+84668915 None:intergenic
TCCACTTGAAGATCTTCAAT+GGG 0.491617 4.2:+84668554 None:intergenic
AGCTTTAAAATGGACCCTAA+TGG 0.492701 4.2:+84669073 None:intergenic
CTTGTTTAGGAAGATCCAAA+AGG 0.511212 4.2:+84668358 None:intergenic
AACTCGTTTGCTGGATGTGT+TGG 0.520836 4.2:-84669040 MS.gene70046:CDS
CAAAATCTCCGAACATGTCA+AGG 0.523947 4.2:-84668691 MS.gene70046:CDS
ACTACATATTATTATGGTAC+AGG 0.525179 4.2:-84668800 MS.gene70046:CDS
CATGTCAAGGGAAGGTGGTG+TGG 0.534164 4.2:-84668678 MS.gene70046:CDS
CTGATGAAGATTGGTTAGGC+AGG 0.539982 4.2:+84669233 None:intergenic
TATACCACATAAATATATCA+AGG 0.540609 4.2:-84668777 MS.gene70046:CDS
ACGGCTGATGAAGATTGGTT+AGG 0.542485 4.2:+84669229 None:intergenic
CTTGAAGATCTTCAATGGGA+AGG 0.557870 4.2:+84668558 None:intergenic
ATGTCAAGGGAAGGTGGTGT+GGG 0.560436 4.2:-84668677 MS.gene70046:CDS
CAAGTGGAGGTGATAAGCAT+TGG 0.573003 4.2:-84668539 MS.gene70046:CDS
AGAGTAAAACCTTGCACAAG+TGG 0.576824 4.2:-84669205 MS.gene70046:CDS
AGATTAGCATTATACGGTGT+AGG 0.576913 4.2:-84668644 MS.gene70046:CDS
CCACATAAATATATCAAGGA+AGG 0.577989 4.2:-84668773 MS.gene70046:CDS
TGGACCCTAATGGCATGGTA+AGG 0.578120 4.2:+84669083 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.582155 4.2:-84669020 MS.gene70046:CDS
GTTTCCCAGCCCACTTGTCA+AGG 0.584219 4.2:+84668380 None:intergenic
CCAAATTTACCTTGACAAGT+GGG 0.586402 4.2:-84668389 MS.gene70046:CDS
TTAGTGCCGCCACTTGTGCA+AGG 0.589354 4.2:+84669196 None:intergenic
TAAAATGGACCCTAATGGCA+TGG 0.595393 4.2:+84669078 None:intergenic
TCCCATTGAAGATCTTCAAG+TGG 0.597466 4.2:-84668555 MS.gene70046:CDS
CCGAACATGTCAAGGGAAGG+TGG 0.605703 4.2:-84668683 MS.gene70046:CDS
CATTGAAGATCTTCAAGTGG+AGG 0.606584 4.2:-84668552 MS.gene70046:CDS
GAAGTCAACAAAATCACCGT+TGG 0.609986 4.2:+84669117 None:intergenic
GTACCATAATAATATGTAGT+GGG 0.617649 4.2:+84668884 None:intergenic
GTACCATAATAATATGTAGT+GGG 0.621257 4.2:+84668803 None:intergenic
AAAATCTCCGAACATGTCAA+GGG 0.622631 4.2:-84668690 MS.gene70046:CDS
TGTACCATAATAATATGTAG+TGG 0.631710 4.2:+84668802 None:intergenic
TCTCCGAACATGTCAAGGGA+AGG 0.632004 4.2:-84668686 MS.gene70046:CDS
AAAAGTAGCTCCAAGTACAA+AGG 0.632301 4.2:-84669259 MS.gene70046:CDS
AGTACCATAATAATATGTAG+TGG 0.637800 4.2:+84668883 None:intergenic
AGAACTTGGACTCTTCATTG+TGG 0.642932 4.2:-84668997 MS.gene70046:CDS
TTTAAATCAGGAATGTTGAG+GGG 0.644292 4.2:+84668911 None:intergenic
ATCATCTTTGTAGAAGCCAA+CGG 0.648266 4.2:-84669133 MS.gene70046:CDS
GTAAAACCTTGCACAAGTGG+CGG 0.651940 4.2:-84669202 MS.gene70046:CDS
GATAAGCATTGGTGAAGCCG+AGG 0.652499 4.2:-84668528 MS.gene70046:intron
CCATAATAATATGTAGTGGG+CGG 0.667025 4.2:+84668887 None:intergenic
TGGTTTGGACAACCAAAATG+TGG 0.677088 4.2:+84668935 None:intergenic
TGGACGTCAAGAATGCTCCA+AGG 0.688603 4.2:+84668409 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCTTTCAAATATAAA+AGG + chr4.2:84669051-84669070 None:intergenic 10.0%
!! TATACCACATAAATATATCA+AGG - chr4.2:84668853-84668872 MS.gene70046:CDS 20.0%
! ACTACATATTATTATGGTAC+AGG - chr4.2:84668749-84668768 MS.gene70046:CDS 25.0%
! ACTACATATTATTATGGTAC+TGG - chr4.2:84668749-84668768 MS.gene70046:CDS 25.0%
! AGTACCATAATAATATGTAG+TGG + chr4.2:84668750-84668769 None:intergenic 25.0%
! GTACCATAATAATATGTAGT+GGG + chr4.2:84668749-84668768 None:intergenic 25.0%
! TGTACCATAATAATATGTAG+TGG + chr4.2:84668831-84668850 None:intergenic 25.0%
!! CAAGAGTTTTGCATTAAAAA+TGG + chr4.2:84668906-84668925 None:intergenic 25.0%
!! TTTTAAATCAGGAATGTTGA+GGG + chr4.2:84668723-84668742 None:intergenic 25.0%
!!! GTTTTAAATCAGAAATGTTG+AGG + chr4.2:84668805-84668824 None:intergenic 25.0%
AATCAGAAATGTTGAGGAAT+TGG + chr4.2:84668799-84668818 None:intergenic 30.0%
ACAAGCAAAAGGAATCAAAA+AGG + chr4.2:84669183-84669202 None:intergenic 30.0%
CCACATAAATATATCAAGGA+AGG - chr4.2:84668857-84668876 MS.gene70046:CDS 30.0%
CCTTCCTTGATATATTTATG+TGG + chr4.2:84668860-84668879 None:intergenic 30.0%
TTTAAATCAGGAATGTTGAG+GGG + chr4.2:84668722-84668741 None:intergenic 30.0%
!! ACCATCTGAAAGCTTTAAAA+TGG + chr4.2:84668570-84668589 None:intergenic 30.0%
!! GTTTTAAATCAGGAATGTTG+AGG + chr4.2:84668724-84668743 None:intergenic 30.0%
!! TTTTTGATTCCTTTTGCTTG+TGG - chr4.2:84669182-84669201 MS.gene70046:CDS 30.0%
!!! TCCATTTTAAAGCTTTCAGA+TGG - chr4.2:84668566-84668585 MS.gene70046:CDS 30.0%
AAAAGTAGCTCCAAGTACAA+AGG - chr4.2:84668371-84668390 MS.gene70046:CDS 35.0%
AAAATCTCCGAACATGTCAA+GGG - chr4.2:84668940-84668959 MS.gene70046:CDS 35.0%
AAATAATAGCCACCTTACCT+CGG + chr4.2:84669122-84669141 None:intergenic 35.0%
AGATTAGCATTATACGGTGT+AGG - chr4.2:84668986-84669005 MS.gene70046:CDS 35.0%
ATCATCTTTGTAGAAGCCAA+CGG - chr4.2:84668497-84668516 MS.gene70046:intron 35.0%
CCAAATTTACCTTGACAAGT+GGG - chr4.2:84669241-84669260 MS.gene70046:CDS 35.0%
CTGCCCACTACATATTATTA+TGG - chr4.2:84668824-84668843 MS.gene70046:CDS 35.0%
CTTGTTTAGGAAGATCCAAA+AGG + chr4.2:84669275-84669294 None:intergenic 35.0%
GAAATGTTGAGGAATTGGTT+TGG + chr4.2:84668794-84668813 None:intergenic 35.0%
GTGCAGAGATTAGCATTATA+CGG - chr4.2:84668980-84668999 MS.gene70046:CDS 35.0%
TCCAAATTTACCTTGACAAG+TGG - chr4.2:84669240-84669259 MS.gene70046:CDS 35.0%
TCCACTTGAAGATCTTCAAT+GGG + chr4.2:84669079-84669098 None:intergenic 35.0%
! CCATAATAATATGTAGTGGG+CGG + chr4.2:84668746-84668765 None:intergenic 35.0%
! TGGTAATTTGTACAAGAGTG+TGG - chr4.2:84668610-84668629 MS.gene70046:CDS 35.0%
! TGGTTTGGACAACCAAAATT+TGG + chr4.2:84668779-84668798 None:intergenic 35.0%
! TGTGCAAGGTTTTACTCTAA+CGG + chr4.2:84668423-84668442 None:intergenic 35.0%
!! AGCTTTAAAATGGACCCTAA+TGG + chr4.2:84668560-84668579 None:intergenic 35.0%
AGAACTTGGACTCTTCATTG+TGG - chr4.2:84668633-84668652 MS.gene70046:CDS 40.0%
AGAGTAAAACCTTGCACAAG+TGG - chr4.2:84668425-84668444 MS.gene70046:intron 40.0%
CAAAATCTCCGAACATGTCA+AGG - chr4.2:84668939-84668958 MS.gene70046:CDS 40.0%
CATTGAAGATCTTCAAGTGG+AGG - chr4.2:84669078-84669097 MS.gene70046:CDS 40.0%
CCCACTTGTCAAGGTAAATT+TGG + chr4.2:84669244-84669263 None:intergenic 40.0%
CCGCCCACTACATATTATTA+TGG - chr4.2:84668743-84668762 MS.gene70046:CDS 40.0%
CGGCACTAAAATGGATGTTA+AGG - chr4.2:84668448-84668467 MS.gene70046:intron 40.0%
CTCCACTTGAAGATCTTCAA+TGG + chr4.2:84669080-84669099 None:intergenic 40.0%
CTTGAAGATCTTCAATGGGA+AGG + chr4.2:84669075-84669094 None:intergenic 40.0%
GAAGTCAACAAAATCACCGT+TGG + chr4.2:84668516-84668535 None:intergenic 40.0%
TAAAATGGACCCTAATGGCA+TGG + chr4.2:84668555-84668574 None:intergenic 40.0%
TCCCATTGAAGATCTTCAAG+TGG - chr4.2:84669075-84669094 MS.gene70046:CDS 40.0%
! ATTCCTTTTGCTTGTGGTGT+AGG - chr4.2:84669188-84669207 MS.gene70046:CDS 40.0%
! CTTGACATGTTCGGAGATTT+TGG + chr4.2:84668941-84668960 None:intergenic 40.0%
! TGGTTTGGACAACCAAAATG+TGG + chr4.2:84668698-84668717 None:intergenic 40.0%
!!! GGTACTGGTGTACCAAATTT+TGG - chr4.2:84668764-84668783 MS.gene70046:CDS 40.0%
AGCTTCCTTACCATGCCATT+AGG - chr4.2:84668542-84668561 MS.gene70046:CDS 45.0%
ATTTACCTTGACAAGTGGGC+TGG - chr4.2:84669245-84669264 MS.gene70046:CDS 45.0%
CAAGTGGAGGTGATAAGCAT+TGG - chr4.2:84669091-84669110 MS.gene70046:CDS 45.0%
CTCTAACGGCTGATGAAGAT+TGG + chr4.2:84668409-84668428 None:intergenic 45.0%
GCTTCCTTACCATGCCATTA+GGG - chr4.2:84668543-84668562 MS.gene70046:CDS 45.0%
GTAAAACCTTGCACAAGTGG+CGG - chr4.2:84668428-84668447 MS.gene70046:intron 45.0%
TCAGATGGTAACTCGTTTGC+TGG - chr4.2:84668581-84668600 MS.gene70046:CDS 45.0%
! AACTCGTTTGCTGGATGTGT+TGG - chr4.2:84668590-84668609 MS.gene70046:CDS 45.0%
! ACGGCTGATGAAGATTGGTT+AGG + chr4.2:84668404-84668423 None:intergenic 45.0%
! CTGATGAAGATTGGTTAGGC+AGG + chr4.2:84668400-84668419 None:intergenic 45.0%
! GTACAAGAGTGTGGAGAACT+TGG - chr4.2:84668619-84668638 MS.gene70046:CDS 45.0%
! TTTACCTTGACAAGTGGGCT+GGG - chr4.2:84669246-84669265 MS.gene70046:CDS 45.0%
!!! GTAGTGGGCGGTTTTAAATC+AGG + chr4.2:84668734-84668753 None:intergenic 45.0%
AATCAGGAATGTTGAGGGGC+TGG + chr4.2:84668718-84668737 None:intergenic 50.0%
AGGCAGGTTGCCTTTGTACT+TGG + chr4.2:84668384-84668403 None:intergenic 50.0%
ATGTCAAGGGAAGGTGGTGT+GGG - chr4.2:84668953-84668972 MS.gene70046:CDS 50.0%
CACAAGTGGCGGCACTAAAA+TGG - chr4.2:84668439-84668458 MS.gene70046:intron 50.0%
CCACCTTCCCTTGACATGTT+CGG + chr4.2:84668950-84668969 None:intergenic 50.0%
GAGCCTACACCACAAGCAAA+AGG + chr4.2:84669194-84669213 None:intergenic 50.0%
TCTCCGAACATGTCAAGGGA+AGG - chr4.2:84668944-84668963 MS.gene70046:CDS 50.0%
TGGACCCTAATGGCATGGTA+AGG + chr4.2:84668550-84668569 None:intergenic 50.0%
TGGACGTCAAGAATGCTCCA+AGG + chr4.2:84669224-84669243 None:intergenic 50.0%
! CGCACTGTGTTACTCAATCC+TGG - chr4.2:84668665-84668684 MS.gene70046:CDS 50.0%
!! GATAAGCATTGGTGAAGCCG+AGG - chr4.2:84669102-84669121 MS.gene70046:CDS 50.0%
!!! AAGTGGGCTGGGAAACCTTT+TGG - chr4.2:84669257-84669276 MS.gene70046:CDS 50.0%
CATGTCAAGGGAAGGTGGTG+TGG - chr4.2:84668952-84668971 MS.gene70046:CDS 55.0%
CCGAACATGTCAAGGGAAGG+TGG - chr4.2:84668947-84668966 MS.gene70046:CDS 55.0%
GTTTCCCAGCCCACTTGTCA+AGG + chr4.2:84669253-84669272 None:intergenic 55.0%
TTAGTGCCGCCACTTGTGCA+AGG + chr4.2:84668437-84668456 None:intergenic 55.0%
TTGGTGAAGCCGAGGTAAGG+TGG - chr4.2:84669110-84669129 MS.gene70046:CDS 55.0%
! GCATTGGTGAAGCCGAGGTA+AGG - chr4.2:84669107-84669126 MS.gene70046:CDS 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG + chr4.2:84668713-84668732 None:intergenic 55.0%
!! GTGTAGGCTCTGAGCATCCT+TGG - chr4.2:84669204-84669223 MS.gene70046:CDS 55.0%
! CCTGGCGCAGCACCACATTT+TGG - chr4.2:84668683-84668702 MS.gene70046:CDS 60.0%
!! CCAAAATGTGGTGCTGCGCC+AGG + chr4.2:84668686-84668705 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84668353 84669299 84668353 ID=MS.gene70046
chr4.2 mRNA 84668353 84669299 84668353 ID=MS.gene70046.t1;Parent=MS.gene70046
chr4.2 exon 84668529 84669299 84668529 ID=MS.gene70046.t1.exon1;Parent=MS.gene70046.t1
chr4.2 CDS 84668529 84669299 84668529 ID=cds.MS.gene70046.t1;Parent=MS.gene70046.t1
chr4.2 exon 84668353 84668442 84668353 ID=MS.gene70046.t1.exon2;Parent=MS.gene70046.t1
chr4.2 CDS 84668353 84668442 84668353 ID=cds.MS.gene70046.t1;Parent=MS.gene70046.t1
Gene Sequence

>MS.gene70046

ATGACAAATACAATTTTGAAAAGTAGCTCCAAGTACAAAGGCAACCTGCCTAACCAATCTTCATCAGCCGTTAGAGTAAAACCTTGCACAAGTGGCGGCACTAAAATGGATGTTAAGGTAGTTCGAAGTATATCTCAGAAAAAGATCATCTTTGTAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTAGGGTCCATTTTAAAGCTTTCAGATGGTAACTCGTTTGCTGGATGTGTTGGTAATTTGTACAAGAGTGTGGAGAACTTGGACTCTTCATTGTGGACTGACTCACGCACTGTGTTACTCAATCCTGGCGCAGCACCACATTTTGGTTGTCCAAACCAGCCCCTCAACATTCCTGATTTAAAACCGCCCACTACATATTATTATGGTACTGGTGTACCAAATTTTGGTTGTCCAAACCAATTCCTCAACATTTCTGATTTAAAACTGCCCACTACATATTATTATGGTACAGGTATACCACATAAATATATCAAGGAAGGAGTGATTTCTAAGACAAAAGAATCCATTTTTAATGCAAAACTCTTGACTGCACTTGATCCAAAATCTCCGAACATGTCAAGGGAAGGTGGTGTGGGATTTGTGCAGAGATTAGCATTATACGGTGTAGGAGATGATCTAAAAGTAAAATCACTTTCAGCTAATTCTTTCCTTTTATATTTGAAAGAATTAAGCCTTCCCATTGAAGATCTTCAAGTGGAGGTGATAAGCATTGGTGAAGCCGAGGTAAGGTGGCTATTATTTTATCAGTGAATTTATAAATTTTTTCGTTTTTATTTTAACCTTTTTGATTCCTTTTGCTTGTGGTGTAGGCTCTGAGCATCCTTGGAGCATTCTTGACGTCCAAATTTACCTTGACAAGTGGGCTGGGAAACCTTTTGGATCTTCCTAAACAAGAATAA
Protein sequence

>MS.gene70046.t1

MTNTILKSSSKYKGNLPNQSSSAVRVKPCTSGGTKMDVKVVRSISQKKIIFVEANGDFVDFIFSFLTMPLGSILKLSDGNSFAGCVGNLYKSVENLDSSLWTDSRTVLLNPGAAPHFGCPNQPLNIPDLKPPTTYYYGTGVPNFGCPNQFLNISDLKLPTTYYYGTGIPHKYIKEGVISKTKESIFNAKLLTALDPKSPNMSREGGVGFVQRLALYGVGDDLKVKSLSANSFLLYLKELSLPIEDLQVEVISIGEAEALSILGAFLTSKFTLTSGLGNLLDLPKQE