AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene70042

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70042.t1 MTR_4g017610 87.579 475 58 1 1 474 1 475 0.0 835
MS.gene70042.t1 MTR_4g017410 61.245 498 149 9 8 478 4 484 0.0 555
MS.gene70042.t1 MTR_4g017440 60.800 500 169 7 4 478 5 502 0.0 548
MS.gene70042.t1 MTR_4g017830 57.769 502 184 7 3 478 2 501 0.0 547
MS.gene70042.t1 MTR_4g017550 62.025 474 151 10 27 476 3 471 0.0 532
MS.gene70042.t1 MTR_4g017550 27.016 248 131 9 16 241 250 469 5.20e-11 65.1
MS.gene70042.t1 MTR_4g019340 58.958 480 161 9 12 468 37 503 0.0 517
MS.gene70042.t1 MTR_4g017470 53.725 510 164 10 1 478 1 470 7.16e-161 465
MS.gene70042.t1 MTR_4g019310 39.087 504 267 14 1 478 1 490 9.90e-99 306
MS.gene70042.t1 MTR_7g066570 36.399 511 262 17 4 482 5 484 1.97e-75 248
MS.gene70042.t1 MTR_7g066340 34.073 496 267 14 3 480 2 455 8.39e-75 243
MS.gene70042.t1 MTR_7g058710 34.325 504 257 14 16 482 15 481 7.48e-74 241
MS.gene70042.t1 MTR_7g058710 26.484 219 140 4 267 471 10 221 3.03e-14 75.5
MS.gene70042.t1 MTR_7g066260 33.936 498 261 15 6 478 8 462 1.39e-73 241
MS.gene70042.t1 MTR_7g066350 34.356 489 254 11 7 474 3 445 5.78e-71 233
MS.gene70042.t1 MTR_7g012680 32.874 508 255 13 4 485 5 452 2.84e-69 228
MS.gene70042.t1 MTR_7g066430 33.936 498 275 15 16 478 7 485 8.86e-69 228
MS.gene70042.t1 MTR_7g066430 24.797 246 159 4 7 241 251 481 1.79e-13 72.8
MS.gene70042.t1 MTR_7g066410 33.269 520 268 16 20 481 17 515 7.58e-67 224
MS.gene70042.t1 MTR_7g066410 26.378 254 155 7 2 241 273 508 7.07e-12 68.2
MS.gene70042.t1 MTR_1g041315 32.914 477 262 15 9 462 10 451 7.03e-64 215
MS.gene70042.t1 MTR_7g062280 32.939 507 276 16 16 485 13 492 2.21e-62 211
MS.gene70042.t1 MTR_7g066400 32.712 483 239 14 15 474 14 433 1.20e-61 208
MS.gene70042.t1 MTR_7g066360 33.265 487 267 13 6 478 7 449 3.73e-60 205
MS.gene70042.t1 MTR_7g066290 32.591 494 244 12 6 478 8 433 1.56e-58 199
MS.gene70042.t1 MTR_7g063660 32.587 491 286 15 16 485 12 478 5.27e-58 199
MS.gene70042.t1 MTR_7g063660 32.370 173 100 5 269 428 9 177 1.07e-12 70.5
MS.gene70042.t1 MTR_7g062300 32.934 501 278 17 2 477 5 472 9.47e-58 198
MS.gene70042.t1 MTR_7g063900 33.066 499 269 15 16 478 13 482 1.81e-56 196
MS.gene70042.t1 MTR_7g063900 27.556 225 132 7 269 471 10 225 7.90e-11 64.7
MS.gene70042.t1 MTR_7g066580 31.493 489 254 15 20 475 2 442 2.48e-56 194
MS.gene70042.t1 MTR_7g066580 28.163 245 148 7 10 241 212 441 4.14e-11 65.5
MS.gene70042.t1 MTR_7g063880 31.765 510 276 18 2 484 4 468 1.10e-55 193
MS.gene70042.t1 MTR_7g063840 30.357 504 295 15 6 485 3 474 1.43e-54 190
MS.gene70042.t1 MTR_7g063890 31.791 497 289 14 16 484 30 504 3.65e-54 189
MS.gene70042.t1 MTR_7g063820 33.066 499 279 19 16 485 13 485 9.33e-54 188
MS.gene70042.t1 MTR_7g063920 31.048 496 272 13 1 485 1 437 1.66e-52 184
MS.gene70042.t1 MTR_7g058700 30.691 492 222 17 19 482 525 925 8.66e-52 189
MS.gene70042.t1 MTR_7g063830 31.337 501 258 19 1 478 1 438 5.05e-49 174
MS.gene70042.t1 MTR_7g066310 30.021 473 222 13 16 478 10 383 5.08e-49 173
MS.gene70042.t1 MTR_2g029400 29.303 488 221 15 9 466 10 403 6.52e-47 168
MS.gene70042.t1 MTR_3g065200 28.687 495 253 17 16 484 6 426 3.56e-45 164
MS.gene70042.t1 MTR_3g007740 30.085 472 280 12 16 478 12 442 2.98e-44 161
MS.gene70042.t1 MTR_4g019240 61.789 123 45 1 1 123 1 121 3.39e-44 154
MS.gene70042.t1 MTR_7g063670 29.688 512 276 19 1 485 4 458 1.76e-43 160
MS.gene70042.t1 MTR_7g066480 35.347 331 177 11 16 332 19 326 1.51e-40 151
MS.gene70042.t1 MTR_7g066480 26.577 222 138 8 268 471 15 229 8.36e-11 64.3
MS.gene70042.t1 MTR_7g066490 32.822 326 176 10 16 331 19 311 1.62e-36 140
MS.gene70042.t1 MTR_1g014060 35.242 227 124 6 22 241 9 219 2.08e-30 122
MS.gene70042.t1 MTR_1g014060 32.663 199 105 4 299 485 210 391 4.23e-20 92.4
MS.gene70042.t1 MTR_7g066680 31.939 263 151 7 216 457 31 286 5.78e-26 108
MS.gene70042.t1 MTR_7g066280 31.818 242 123 11 100 330 1 211 1.69e-21 97.1
MS.gene70042.t1 MTR_7g066280 41.463 82 47 1 398 478 309 390 7.43e-12 67.4
MS.gene70042.t1 MTR_7g062270 36.923 195 95 9 8 193 5 180 8.77e-21 94.4
MS.gene70042.t1 MTR_8g023240 24.440 491 292 17 13 470 37 481 3.97e-20 94.0
MS.gene70042.t1 MTR_7g063720 29.870 231 126 5 259 474 13 222 1.35e-18 85.5
MS.gene70042.t1 MTR_8g445860 27.642 246 145 6 11 243 11 236 2.14e-16 79.0
MS.gene70042.t1 MTR_8g445860 28.384 229 130 8 269 472 13 232 7.28e-16 77.4
MS.gene70042.t1 MTR_1g115440 29.333 225 138 6 268 472 10 233 1.47e-15 76.6
MS.gene70042.t1 MTR_1g115440 27.426 237 150 6 16 243 14 237 1.57e-14 73.6
MS.gene70042.t1 MTR_0150s0010 29.646 226 136 8 268 472 9 232 1.87e-14 73.2
MS.gene70042.t1 MTR_0150s0010 29.167 240 142 8 16 243 13 236 1.02e-11 65.5
MS.gene70042.t1 MTR_6g006800 72.917 48 11 1 1 48 1 46 4.81e-14 67.4
MS.gene70042.t1 MTR_4g019330 31.892 185 78 5 283 466 32 169 8.55e-14 70.5
MS.gene70042.t1 MTR_1g115455 28.514 249 128 10 16 243 12 231 3.57e-13 69.3
MS.gene70042.t1 MTR_7g100420 27.957 279 156 9 222 474 134 393 1.33e-12 69.7
MS.gene70042.t1 MTR_1g115435 26.582 237 143 6 11 247 5 210 4.58e-12 65.9
MS.gene70042.t1 MTR_8g445920 42.857 63 36 0 268 330 9 71 4.36e-11 60.5
MS.gene70042.t1 MTR_8g044890 42.857 63 36 0 268 330 9 71 4.36e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene70042.t1 AT3G09140 31.349 504 293 16 7 474 3 489 1.18e-59 204
MS.gene70042.t1 AT5G01120 30.894 492 301 14 10 474 6 485 4.48e-57 197
MS.gene70042.t1 AT5G43240 32.460 496 280 20 16 474 12 489 1.58e-55 194
MS.gene70042.t1 AT5G43240 32.460 496 280 20 16 474 12 489 1.58e-55 194
MS.gene70042.t1 AT5G43240 32.460 496 280 20 16 474 12 489 1.58e-55 194
MS.gene70042.t1 AT3G09140 29.699 532 293 17 7 474 3 517 1.05e-53 189
MS.gene70042.t1 AT5G01130 30.408 490 276 18 6 478 3 444 9.11e-51 179
MS.gene70042.t1 AT5G01150 29.091 495 302 13 7 474 3 475 1.45e-49 177
MS.gene70042.t1 AT5G01140 30.579 484 281 15 7 474 3 447 7.80e-49 174
MS.gene70042.t1 AT3G09110 33.141 347 205 10 10 345 6 336 2.22e-48 170
MS.gene70042.t1 AT5G37320 30.061 489 285 18 20 478 18 479 4.07e-44 162
MS.gene70042.t1 AT3G09120 33.837 331 182 12 10 323 3 313 9.27e-39 143
MS.gene70042.t1 AT3G09120 26.872 227 146 5 266 472 3 229 1.02e-13 72.4
MS.gene70042.t1 AT5G43240 29.885 348 206 13 157 474 6 345 2.35e-25 107

Find 102 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTCTTACGAATACAATTT+TGG 0.148306 4.2:-84635910 MS.gene70042:CDS
GAATCCCCTACTTATTATTA+TGG 0.206471 4.2:-84635543 MS.gene70042:CDS
TAATAGACAACCTAAGAATT+TGG 0.262420 4.2:-84636670 MS.gene70042:CDS
CAATTATAAGATAAATAAAA+TGG 0.268027 4.2:-84635826 MS.gene70042:CDS
CATCGTCTGAGTTGAGGTTT+TGG 0.281834 4.2:+84636874 None:intergenic
CAGGGGAATCATGCCTCTAA+TGG 0.286513 4.2:-84636636 MS.gene70042:CDS
GGGTATTAAAGTCTTTATAA+TGG 0.290144 4.2:+84635698 None:intergenic
AGTTTCCTTACCATTCCATT+AGG 0.290188 4.2:-84635723 MS.gene70042:CDS
CCCTCAGAGTCCAAATTCTT+AGG 0.309465 4.2:+84636660 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.310647 4.2:+84636672 None:intergenic
ATTCTTCTCCATCAACATAT+TGG 0.310876 4.2:+84635853 None:intergenic
TCAGAATCATCACCATTCTT+TGG 0.319703 4.2:-84636924 MS.gene70042:CDS
ATGCAGATGTTGCTGGAATC+AGG 0.320991 4.2:-84636534 MS.gene70042:CDS
TCCAAAATTGTATTCGTAAG+AGG 0.327361 4.2:+84635909 None:intergenic
AGCAACATTTGCTTACATAA+AGG 0.329982 4.2:+84636840 None:intergenic
TGTTGTTGGTGGTTGCTAAA+AGG 0.334739 4.2:+84636964 None:intergenic
GCAACATTTGCTTACATAAA+GGG 0.341137 4.2:+84636841 None:intergenic
ACCTCAACTCAGACGATGTT+TGG 0.342611 4.2:-84636869 MS.gene70042:CDS
TGTATCAAACACCCCATTAG+AGG 0.344943 4.2:+84636623 None:intergenic
CACATGTATCAATTGTTAAT+CGG 0.352686 4.2:-84635461 MS.gene70042:CDS
ATATGTTGATGGAGAAGAAT+TGG 0.357603 4.2:-84635850 MS.gene70042:CDS
GTTTCCTTACCATTCCATTA+GGG 0.375690 4.2:-84635722 MS.gene70042:CDS
AGGGGAATCATGCCTCTAAT+GGG 0.380156 4.2:-84636635 MS.gene70042:CDS
AACGTCTAAGTCCACTAGTC+AGG 0.385798 4.2:+84635119 None:intergenic
GAACTCAATGCAGATGTTGC+TGG 0.386915 4.2:-84636541 MS.gene70042:CDS
GAATTTGGACTCTGAGGGTC+AGG 0.397162 4.2:-84636655 MS.gene70042:CDS
TGAATGTCAAGGGGTTGCTT+TGG 0.403432 4.2:+84635573 None:intergenic
GTCTTTATAATGGACCCTAA+TGG 0.418519 4.2:+84635708 None:intergenic
TCTTACCTCCCTTTCATCAA+TGG 0.421763 4.2:+84635266 None:intergenic
GAAGAGAGCAGCATTATATA+TGG 0.440249 4.2:-84635391 MS.gene70042:CDS
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.2:-84636999 MS.gene70042:CDS
AAGAGGCTCATGAGAGGTTA+AGG 0.445820 4.2:+84635926 None:intergenic
AATCCTACAGCACCTTCTCT+TGG 0.447362 4.2:+84635417 None:intergenic
ATTCTCTTCCATTTGAAAGC+TGG 0.450179 4.2:+84635091 None:intergenic
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.2:+84636994 None:intergenic
CAGACATAAAACTTACTTGA+CGG 0.460722 4.2:+84636753 None:intergenic
TGAATACCATAATAATAAGT+AGG 0.466481 4.2:+84635537 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4.2:+84635937 None:intergenic
TATGTTGATGGAGAAGAATT+GGG 0.477595 4.2:-84635849 MS.gene70042:CDS
GTACAACAGTGTGAAGAACT+TGG 0.479946 4.2:-84635646 MS.gene70042:CDS
GAATACCATAATAATAAGTA+GGG 0.484408 4.2:+84635538 None:intergenic
ATCCTACAGCACCTTCTCTT+GGG 0.485199 4.2:+84635418 None:intergenic
CCACGGAACCAATATGTTGA+TGG 0.488534 4.2:-84635861 MS.gene70042:CDS
CGTGATTACCATTGATGAAA+GGG 0.498975 4.2:-84635274 MS.gene70042:CDS
TTGTCGATCATTGTTGTTGG+TGG 0.499724 4.2:+84636953 None:intergenic
ATTGAGTTCAGCAAGGAACT+TGG 0.501314 4.2:+84636555 None:intergenic
AACACATCCAGGAAAATTGT+TGG 0.509207 4.2:+84635677 None:intergenic
CACACAAAGCATTGGCAAGA+AGG 0.511399 4.2:-84636481 MS.gene70042:CDS
CGAAACCTTGACTGCACCTG+CGG 0.512942 4.2:-84636702 MS.gene70042:CDS
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4.2:+84636675 None:intergenic
TTAATTCTTTCAGATATGAA+AGG 0.526183 4.2:+84635325 None:intergenic
CCATCAACATATTGGTTCCG+TGG 0.536459 4.2:+84635861 None:intergenic
TGCAGATGTTGCTGGAATCA+GGG 0.536790 4.2:-84636533 MS.gene70042:CDS
TGTTTGATGTTGCCAAAGAA+TGG 0.539653 4.2:+84636912 None:intergenic
TACAAATTACCAACACATCC+AGG 0.541284 4.2:+84635666 None:intergenic
ACACATCCAGGAAAATTGTT+GGG 0.541913 4.2:+84635678 None:intergenic
TCCAAACATCGTCTGAGTTG+AGG 0.543809 4.2:+84636868 None:intergenic
TTGTTCTTGTTGAGAAGTCA+TGG 0.547094 4.2:+84637124 None:intergenic
ATCATCATTGCAGAAGCTGA+TGG 0.549559 4.2:-84635768 MS.gene70042:CDS
ATTCATGTGAATCACTATGC+AGG 0.552422 4.2:-84636806 MS.gene70042:CDS
CCGTGATTACCATTGATGAA+AGG 0.554883 4.2:-84635275 MS.gene70042:CDS
TCAAAATTTACCCTGACTAG+TGG 0.555267 4.2:-84635130 MS.gene70042:CDS
TGATGATTATAATACAATAG+AGG 0.560654 4.2:-84635499 MS.gene70042:CDS
TTCTAAAGGATGAATGTCAA+GGG 0.563362 4.2:+84635563 None:intergenic
GCAAAGTGTGGTGCTATACA+AGG 0.564905 4.2:+84635600 None:intergenic
ACCTAAGAATTTGGACTCTG+AGG 0.575501 4.2:-84636661 MS.gene70042:CDS
AGTGATTCACATGAATTCCT+CGG 0.577391 4.2:+84636813 None:intergenic
TGGACCCTAATGGAATGGTA+AGG 0.578424 4.2:+84635718 None:intergenic
TATAATGGACCCTAATGGAA+TGG 0.581198 4.2:+84635713 None:intergenic
AGGAAAGAATTAGCAGCAAG+TGG 0.583512 4.2:+84635345 None:intergenic
TAATAAGTAGGGGATTCTAA+AGG 0.590723 4.2:+84635549 None:intergenic
AGCAAATGTTGCTGCATCCG+AGG 0.591888 4.2:-84636830 MS.gene70042:CDS
ACGTCTAAGTCCACTAGTCA+GGG 0.592093 4.2:+84635120 None:intergenic
CCTAAGAATTTGGACTCTGA+GGG 0.592717 4.2:-84636660 MS.gene70042:CDS
CTTCAAGATCATCAAGAGGA+AGG 0.593465 4.2:+84635301 None:intergenic
AATTTGGACTCTGAGGGTCA+GGG 0.595588 4.2:-84636654 MS.gene70042:CDS
ATTTGGACTCTGAGGGTCAG+GGG 0.598326 4.2:-84636653 MS.gene70042:CDS
CCTTTCATCAATGGTAATCA+CGG 0.598872 4.2:+84635275 None:intergenic
GAGGAAGTCACACAAAGCAT+TGG 0.599245 4.2:-84636489 MS.gene70042:CDS
GGATGGAGCAACTCAGACAC+GGG 0.602700 4.2:+84635621 None:intergenic
ATTCTAAAGGATGAATGTCA+AGG 0.609187 4.2:+84635562 None:intergenic
AACGTTTCCCCTCAAAATCA+CGG 0.610398 4.2:-84637087 MS.gene70042:CDS
ATTGAACTTGACCCAAGAGA+AGG 0.610689 4.2:-84635429 MS.gene70042:CDS
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4.2:+84635940 None:intergenic
AGGATGGAGCAACTCAGACA+CGG 0.622529 4.2:+84635620 None:intergenic
TTCAGACTCAACGCAACTAG+AGG 0.625063 4.2:-84636508 MS.gene70042:CDS
TGCTTTGGACAAGCAAAGTG+TGG 0.625304 4.2:+84635588 None:intergenic
AGAGCAGCATTATATATGGT+AGG 0.629947 4.2:-84635387 MS.gene70042:CDS
AGTGTGGTGCTATACAAGGA+TGG 0.638386 4.2:+84635604 None:intergenic
GGGGAATCATGCCTCTAATG+GGG 0.639572 4.2:-84636634 MS.gene70042:CDS
TTGATGTGAAGAATGCTCGA+AGG 0.642051 4.2:+84635149 None:intergenic
CAAAGAACAATGACGAACCA+CGG 0.642115 4.2:-84635878 MS.gene70042:CDS
ACAAAGCATTGGCAAGAAGG+AGG 0.647102 4.2:-84636478 MS.gene70042:intron
GACCCAAGAGAAGGTGCTGT+AGG 0.653060 4.2:-84635420 MS.gene70042:CDS
GATTACCATTGATGAAAGGG+AGG 0.668082 4.2:-84635271 MS.gene70042:intron
TCTAAAGGATGAATGTCAAG+GGG 0.670617 4.2:+84635564 None:intergenic
GTCACTTCAAGATCATCAAG+AGG 0.671503 4.2:+84635297 None:intergenic
AATACCATAATAATAAGTAG+GGG 0.676299 4.2:+84635539 None:intergenic
GGTTGCTAAAAGGCGAACGA+TGG 0.680679 4.2:+84636974 None:intergenic
ATTCGTAAGAGGCTCATGAG+AGG 0.692406 4.2:+84635920 None:intergenic
CATCTGCATTGAGTTCAGCA+AGG 0.698278 4.2:+84636548 None:intergenic
AGGCGAACGATGGTACCAAG+TGG 0.729828 4.2:+84636984 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATAAGATAAATAAAA+TGG - chr4.2:84636371-84636390 MS.gene70042:intron 10.0%
!! ATAAAATGTTAAAACACAAA+TGG + chr4.2:84636225-84636244 None:intergenic 15.0%
!!! AAATTTAAAATCATAGGTTA+AGG + chr4.2:84635863-84635882 None:intergenic 15.0%
!!! TAACCTATGATTTTAAATTT+AGG - chr4.2:84635863-84635882 MS.gene70042:CDS 15.0%
!! AATAAAGAGAAATAGAACTT+TGG + chr4.2:84636156-84636175 None:intergenic 20.0%
!! AATACCATAATAATAAGTAG+GGG + chr4.2:84636661-84636680 None:intergenic 20.0%
!! AGGTTAAAAAAACAGAAAAA+AGG + chr4.2:84636990-84637009 None:intergenic 20.0%
!! CGATATATATAGTTAATACT+TGG - chr4.2:84636005-84636024 MS.gene70042:intron 20.0%
!! GAATACCATAATAATAAGTA+GGG + chr4.2:84636662-84636681 None:intergenic 20.0%
!! TGAATACCATAATAATAAGT+AGG + chr4.2:84636663-84636682 None:intergenic 20.0%
!! TGATGATTATAATACAATAG+AGG - chr4.2:84636698-84636717 MS.gene70042:CDS 20.0%
!! TTAATTCTTTCAGATATGAA+AGG + chr4.2:84636875-84636894 None:intergenic 20.0%
! CACATGTATCAATTGTTAAT+CGG - chr4.2:84636736-84636755 MS.gene70042:CDS 25.0%
! GGAAAAAAAATCAATGGAAT+GGG + chr4.2:84635803-84635822 None:intergenic 25.0%
! GGGTATTAAAGTCTTTATAA+TGG + chr4.2:84636502-84636521 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG - chr4.2:84635527-84635546 MS.gene70042:CDS 25.0%
! TGGAAAAAAAATCAATGGAA+TGG + chr4.2:84635804-84635823 None:intergenic 25.0%
! TTTCGTTGTGAATCTATATT+GGG - chr4.2:84635891-84635910 MS.gene70042:CDS 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG + chr4.2:84635528-84635547 None:intergenic 25.0%
!! GCACCTAAATTTAAAATCAT+AGG + chr4.2:84635869-84635888 None:intergenic 25.0%
!! TATCTAAAACCATTACATTG+TGG - chr4.2:84635961-84635980 MS.gene70042:intron 25.0%
AAGAGAAATAGAACTTTGGT+AGG + chr4.2:84636152-84636171 None:intergenic 30.0%
ACAATTAAAGGGAATCAAAG+AGG + chr4.2:84637010-84637029 None:intergenic 30.0%
AGAGAAATAGAACTTTGGTA+GGG + chr4.2:84636151-84636170 None:intergenic 30.0%
AGCAACATTTGCTTACATAA+AGG + chr4.2:84635360-84635379 None:intergenic 30.0%
ATCTATATTGGGGTAAAATG+CGG - chr4.2:84635902-84635921 MS.gene70042:CDS 30.0%
ATGTTATCTGTTAGTGATAG+AGG - chr4.2:84636043-84636062 MS.gene70042:intron 30.0%
ATTCTTCTCCATCAACATAT+TGG + chr4.2:84636347-84636366 None:intergenic 30.0%
CAAATGGTTAGAATGAAAGT+TGG + chr4.2:84636209-84636228 None:intergenic 30.0%
CAGACATAAAACTTACTTGA+CGG + chr4.2:84635447-84635466 None:intergenic 30.0%
GAATCCCCTACTTATTATTA+TGG - chr4.2:84636654-84636673 MS.gene70042:CDS 30.0%
GCAACATTTGCTTACATAAA+GGG + chr4.2:84635359-84635378 None:intergenic 30.0%
GCAAGTGGAAAAAAAATCAA+TGG + chr4.2:84635809-84635828 None:intergenic 30.0%
GTTTCGTTGTGAATCTATAT+TGG - chr4.2:84635890-84635909 MS.gene70042:CDS 30.0%
TAATAAGTAGGGGATTCTAA+AGG + chr4.2:84636651-84636670 None:intergenic 30.0%
TCCAAAATTGTATTCGTAAG+AGG + chr4.2:84636291-84636310 None:intergenic 30.0%
TCTTTGATTCCCTTTAATTG+TGG - chr4.2:84637009-84637028 MS.gene70042:CDS 30.0%
TTCGTTGTGAATCTATATTG+GGG - chr4.2:84635892-84635911 MS.gene70042:CDS 30.0%
! ATATGTTGATGGAGAAGAAT+TGG - chr4.2:84636347-84636366 MS.gene70042:intron 30.0%
! ATTCTAAAGGATGAATGTCA+AGG + chr4.2:84636638-84636657 None:intergenic 30.0%
! TATGTTGATGGAGAAGAATT+GGG - chr4.2:84636348-84636367 MS.gene70042:intron 30.0%
! TTAATACCCAACAATTTTCC+TGG - chr4.2:84636513-84636532 MS.gene70042:CDS 30.0%
! TTCTAAAGGATGAATGTCAA+GGG + chr4.2:84636637-84636656 None:intergenic 30.0%
! TTTTTGTCGATCATTGTTGT+TGG + chr4.2:84635250-84635269 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG - chr4.2:84635143-84635162 MS.gene70042:CDS 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG - chr4.2:84635767-84635786 MS.gene70042:CDS 30.0%
!!! GTTTTTTACCAGCTTTCAAA+TGG - chr4.2:84637098-84637117 MS.gene70042:CDS 30.0%
AAAGCCTACACCACAATTAA+AGG + chr4.2:84637022-84637041 None:intergenic 35.0%
AACACATCCAGGAAAATTGT+TGG + chr4.2:84636523-84636542 None:intergenic 35.0%
AAGCCTACACCACAATTAAA+GGG + chr4.2:84637021-84637040 None:intergenic 35.0%
ACACATCCAGGAAAATTGTT+GGG + chr4.2:84636522-84636541 None:intergenic 35.0%
AGAGCAGCATTATATATGGT+AGG - chr4.2:84636810-84636829 MS.gene70042:CDS 35.0%
AGTGATTCACATGAATTCCT+CGG + chr4.2:84635387-84635406 None:intergenic 35.0%
AGTTTCCTTACCATTCCATT+AGG - chr4.2:84636474-84636493 MS.gene70042:intron 35.0%
ATTCATGTGAATCACTATGC+AGG - chr4.2:84635391-84635410 MS.gene70042:CDS 35.0%
ATTCCCTTTAATTGTGGTGT+AGG - chr4.2:84637015-84637034 MS.gene70042:CDS 35.0%
ATTCTCTTCCATTTGAAAGC+TGG + chr4.2:84637109-84637128 None:intergenic 35.0%
CCTTTCATCAATGGTAATCA+CGG + chr4.2:84636925-84636944 None:intergenic 35.0%
CGTGATTACCATTGATGAAA+GGG - chr4.2:84636923-84636942 MS.gene70042:CDS 35.0%
GAAGAGAGCAGCATTATATA+TGG - chr4.2:84636806-84636825 MS.gene70042:CDS 35.0%
GTTTCCTTACCATTCCATTA+GGG - chr4.2:84636475-84636494 MS.gene70042:intron 35.0%
TACAAATTACCAACACATCC+AGG + chr4.2:84636534-84636553 None:intergenic 35.0%
TCAAAATTTACCCTGACTAG+TGG - chr4.2:84637067-84637086 MS.gene70042:CDS 35.0%
TCAGAATCATCACCATTCTT+TGG - chr4.2:84635273-84635292 MS.gene70042:CDS 35.0%
TCTAAAGGATGAATGTCAAG+GGG + chr4.2:84636636-84636655 None:intergenic 35.0%
TGTTTGATGTTGCCAAAGAA+TGG + chr4.2:84635288-84635307 None:intergenic 35.0%
! AACAATTTTCCTGGATGTGT+TGG - chr4.2:84636522-84636541 MS.gene70042:CDS 35.0%
! GCCTCTTACGAATACAATTT+TGG - chr4.2:84636287-84636306 MS.gene70042:intron 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG + chr4.2:84635525-84635544 None:intergenic 35.0%
! TTTGTCTACCGTGATTTTGA+GGG + chr4.2:84635121-84635140 None:intergenic 35.0%
!! ATAGAGGTGTCATTAGGAAA+GGG - chr4.2:84636059-84636078 MS.gene70042:intron 35.0%
!! CTTGTTTCTACAATGACCTA+AGG + chr4.2:84635838-84635857 None:intergenic 35.0%
!! GTCTTTATAATGGACCCTAA+TGG + chr4.2:84636492-84636511 None:intergenic 35.0%
!! TATAATGGACCCTAATGGAA+TGG + chr4.2:84636487-84636506 None:intergenic 35.0%
!! TTAGTGATAGAGGTGTCATT+AGG - chr4.2:84636053-84636072 MS.gene70042:intron 35.0%
AACGTTTCCCCTCAAAATCA+CGG - chr4.2:84635110-84635129 MS.gene70042:CDS 40.0%
ACCTAAGAATTTGGACTCTG+AGG - chr4.2:84635536-84635555 MS.gene70042:CDS 40.0%
AGGAAAGAATTAGCAGCAAG+TGG + chr4.2:84636855-84636874 None:intergenic 40.0%
AGGTTAAGGTATACTTCAGG+AGG + chr4.2:84636260-84636279 None:intergenic 40.0%
ATCATCATTGCAGAAGCTGA+TGG - chr4.2:84636429-84636448 MS.gene70042:intron 40.0%
ATTGAACTTGACCCAAGAGA+AGG - chr4.2:84636768-84636787 MS.gene70042:CDS 40.0%
ATTGAGTTCAGCAAGGAACT+TGG + chr4.2:84635645-84635664 None:intergenic 40.0%
CAAAGAACAATGACGAACCA+CGG - chr4.2:84636319-84636338 MS.gene70042:intron 40.0%
CCGTGATTACCATTGATGAA+AGG - chr4.2:84636922-84636941 MS.gene70042:CDS 40.0%
CCTAAGAATTTGGACTCTGA+GGG - chr4.2:84635537-84635556 MS.gene70042:CDS 40.0%
CTTCAAGATCATCAAGAGGA+AGG + chr4.2:84636899-84636918 None:intergenic 40.0%
GAAATAGAACTTTGGTAGGG+TGG + chr4.2:84636148-84636167 None:intergenic 40.0%
GAGAGGTTAAGGTATACTTC+AGG + chr4.2:84636263-84636282 None:intergenic 40.0%
GATTACCATTGATGAAAGGG+AGG - chr4.2:84636926-84636945 MS.gene70042:CDS 40.0%
GGGAACTAGTTAGAACTTAG+AGG - chr4.2:84636079-84636098 MS.gene70042:intron 40.0%
GTACAACAGTGTGAAGAACT+TGG - chr4.2:84636551-84636570 MS.gene70042:CDS 40.0%
GTCACTTCAAGATCATCAAG+AGG + chr4.2:84636903-84636922 None:intergenic 40.0%
TACCTCGACAACGACATTAA+TGG + chr4.2:84635943-84635962 None:intergenic 40.0%
TCAATGGAATGGGTGCATAA+TGG + chr4.2:84635793-84635812 None:intergenic 40.0%
TCATTGCAGCCACAATGTAA+TGG + chr4.2:84635973-84635992 None:intergenic 40.0%
TCTTACCTCCCTTTCATCAA+TGG + chr4.2:84636934-84636953 None:intergenic 40.0%
TGTATCAAACACCCCATTAG+AGG + chr4.2:84635577-84635596 None:intergenic 40.0%
TTCCACTTGCAAATGACCTT+AGG - chr4.2:84635819-84635838 MS.gene70042:CDS 40.0%
TTGATGAAAGGGAGGTAAGA+TGG - chr4.2:84636934-84636953 MS.gene70042:CDS 40.0%
TTGATGTGAAGAATGCTCGA+AGG + chr4.2:84637051-84637070 None:intergenic 40.0%
! GTTTGTCTACCGTGATTTTG+AGG + chr4.2:84635122-84635141 None:intergenic 40.0%
! TTGTCGATCATTGTTGTTGG+TGG + chr4.2:84635247-84635266 None:intergenic 40.0%
! TTGTCTACCGTGATTTTGAG+GGG + chr4.2:84635120-84635139 None:intergenic 40.0%
!! GATAGAGGTGTCATTAGGAA+AGG - chr4.2:84636058-84636077 MS.gene70042:intron 40.0%
!! TGTTGTTGGTGGTTGCTAAA+AGG + chr4.2:84635236-84635255 None:intergenic 40.0%
!!! AGTTGAGGTTTTGGACAGTT+TGG + chr4.2:84635317-84635336 None:intergenic 40.0%
!!! GGTTTTGGACAGTTTGGTAA+AGG + chr4.2:84635311-84635330 None:intergenic 40.0%
AACGTCTAAGTCCACTAGTC+AGG + chr4.2:84637081-84637100 None:intergenic 45.0%
AAGAGGCTCATGAGAGGTTA+AGG + chr4.2:84636274-84636293 None:intergenic 45.0%
AATCCTACAGCACCTTCTCT+TGG + chr4.2:84636783-84636802 None:intergenic 45.0%
ACCTCAACTCAGACGATGTT+TGG - chr4.2:84635328-84635347 MS.gene70042:CDS 45.0%
ACGTCTAAGTCCACTAGTCA+GGG + chr4.2:84637080-84637099 None:intergenic 45.0%
ACTTTGGTAGGGTGGAACAA+TGG + chr4.2:84636140-84636159 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG - chr4.2:84635198-84635217 MS.gene70042:intron 45.0%
AGGGGAATCATGCCTCTAAT+GGG - chr4.2:84635562-84635581 MS.gene70042:CDS 45.0%
ATCCTACAGCACCTTCTCTT+GGG + chr4.2:84636782-84636801 None:intergenic 45.0%
ATGCAGATGTTGCTGGAATC+AGG - chr4.2:84635663-84635682 MS.gene70042:CDS 45.0%
ATTCGTAAGAGGCTCATGAG+AGG + chr4.2:84636280-84636299 None:intergenic 45.0%
CATCTGCATTGAGTTCAGCA+AGG + chr4.2:84635652-84635671 None:intergenic 45.0%
CCACGGAACCAATATGTTGA+TGG - chr4.2:84636336-84636355 MS.gene70042:intron 45.0%
CCCTCAGAGTCCAAATTCTT+AGG + chr4.2:84635540-84635559 None:intergenic 45.0%
GAACTCAATGCAGATGTTGC+TGG - chr4.2:84635656-84635675 MS.gene70042:CDS 45.0%
GACCTAAGGTCATTTGCAAG+TGG + chr4.2:84635824-84635843 None:intergenic 45.0%
GAGGAAGTCACACAAAGCAT+TGG - chr4.2:84635708-84635727 MS.gene70042:CDS 45.0%
GGGTGCATAATGGCCTAAAA+TGG + chr4.2:84635783-84635802 None:intergenic 45.0%
TCCAAACATCGTCTGAGTTG+AGG + chr4.2:84635332-84635351 None:intergenic 45.0%
TGAATGTCAAGGGGTTGCTT+TGG + chr4.2:84636627-84636646 None:intergenic 45.0%
TGCAGATGTTGCTGGAATCA+GGG - chr4.2:84635664-84635683 MS.gene70042:CDS 45.0%
TGCCATTAATGTCGTTGTCG+AGG - chr4.2:84635938-84635957 MS.gene70042:CDS 45.0%
TGGACCCTAATGGAATGGTA+AGG + chr4.2:84636482-84636501 None:intergenic 45.0%
TGGCAAGAAGGAGGTAACTA+AGG - chr4.2:84635728-84635747 MS.gene70042:CDS 45.0%
TGGTACCAAGTGGCAAAGAT+AGG + chr4.2:84635206-84635225 None:intergenic 45.0%
TTCAGACTCAACGCAACTAG+AGG - chr4.2:84635689-84635708 MS.gene70042:CDS 45.0%
! AATTTGGACTCTGAGGGTCA+GGG - chr4.2:84635543-84635562 MS.gene70042:CDS 45.0%
! TCTATTAGGAGGTTTTCCGC+AGG + chr4.2:84635514-84635533 None:intergenic 45.0%
! TGCTTTGGACAAGCAAAGTG+TGG + chr4.2:84636612-84636631 None:intergenic 45.0%
!! ACAAAGCATTGGCAAGAAGG+AGG - chr4.2:84635719-84635738 MS.gene70042:CDS 45.0%
!! AGTGTGGTGCTATACAAGGA+TGG + chr4.2:84636596-84636615 None:intergenic 45.0%
!! CACACAAAGCATTGGCAAGA+AGG - chr4.2:84635716-84635735 MS.gene70042:CDS 45.0%
!! CATCGTCTGAGTTGAGGTTT+TGG + chr4.2:84635326-84635345 None:intergenic 45.0%
!! CCATCAACATATTGGTTCCG+TGG + chr4.2:84636339-84636358 None:intergenic 45.0%
!! GCAAAGTGTGGTGCTATACA+AGG + chr4.2:84636600-84636619 None:intergenic 45.0%
AGCAAATGTTGCTGCATCCG+AGG - chr4.2:84635367-84635386 MS.gene70042:CDS 50.0%
AGGATGGAGCAACTCAGACA+CGG + chr4.2:84636580-84636599 None:intergenic 50.0%
CAGGGGAATCATGCCTCTAA+TGG - chr4.2:84635561-84635580 MS.gene70042:CDS 50.0%
GGGGAATCATGCCTCTAATG+GGG - chr4.2:84635563-84635582 MS.gene70042:CDS 50.0%
GGTTGCTAAAAGGCGAACGA+TGG + chr4.2:84635226-84635245 None:intergenic 50.0%
! ATTTGGACTCTGAGGGTCAG+GGG - chr4.2:84635544-84635563 MS.gene70042:CDS 50.0%
! GAATTTGGACTCTGAGGGTC+AGG - chr4.2:84635542-84635561 MS.gene70042:CDS 50.0%
AGGCGAACGATGGTACCAAG+TGG + chr4.2:84635216-84635235 None:intergenic 55.0%
CGAAACCTTGACTGCACCTG+CGG - chr4.2:84635495-84635514 MS.gene70042:CDS 55.0%
GGATGGAGCAACTCAGACAC+GGG + chr4.2:84636579-84636598 None:intergenic 55.0%
! GTTTTCCGCAGGTGCAGTCA+AGG + chr4.2:84635503-84635522 None:intergenic 55.0%
!! GACCCAAGAGAAGGTGCTGT+AGG - chr4.2:84636777-84636796 MS.gene70042:CDS 55.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 84635075 84637144 84635075 ID=MS.gene70042
chr4.2 mRNA 84635075 84637144 84635075 ID=MS.gene70042.t1;Parent=MS.gene70042
chr4.2 exon 84636479 84637144 84636479 ID=MS.gene70042.t1.exon1;Parent=MS.gene70042.t1
chr4.2 CDS 84636479 84637144 84636479 ID=cds.MS.gene70042.t1;Parent=MS.gene70042.t1
chr4.2 exon 84635272 84635970 84635272 ID=MS.gene70042.t1.exon2;Parent=MS.gene70042.t1
chr4.2 CDS 84635272 84635970 84635272 ID=cds.MS.gene70042.t1;Parent=MS.gene70042.t1
chr4.2 exon 84635075 84635182 84635075 ID=MS.gene70042.t1.exon3;Parent=MS.gene70042.t1
chr4.2 CDS 84635075 84635182 84635075 ID=cds.MS.gene70042.t1;Parent=MS.gene70042.t1
Gene Sequence

>MS.gene70042

ATGACTTCTCAACAAGAACAACAAGAAGAGTCAAAAACGTTTCCCCTCAAAATCACGGTAGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGAGACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCAACCACCAACAACAATGATCGACAAAAACTCTCAGAATCATCACCATTCTTTGGCAACATCAAACACCTTTACCAAACTGTCCAAAACCTCAACTCAGACGATGTTTGGAACAACCCTTTATGTAAGCAAATGTTGCTGCATCCGAGGAATTCATGTGAATCACTATGCAGGAAGTTGTACTTGAATATTGATGATTCTGAACCGTCAAGTAAGTTTTATGTCTGTGATTCTTGTAACAAGTTCACTACTTTTCGAAACCTTGACTGCACCTGCGGAAAACCTCCTAATAGACAACCTAAGAATTTGGACTCTGAGGGTCAGGGGAATCATGCCTCTAATGGGGTGTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAATATTGTGCCAAGTTCCTTGCTGAACTCAATGCAGATGTTGCTGGAATCAGGGATTTCAGACTCAACGCAACTAGAGGAAGTCACACAAAGCATTGGCAAGAAGGAGGTAACTAAGGATGTTGTGTTGTTTTTGTTTGTTGTGTTTCCATTTTAGGCCATTATGCACCCATTCCATTGATTTTTTTTCCACTTGCAAATGACCTTAGGTCATTGTAGAAACAAGATCCTTAACCTATGATTTTAAATTTAGGTGCAGTTTCGTTGTGAATCTATATTGGGGTAAAATGCGGTCGTATTTGCAGTTGCCATTAATGTCGTTGTCGAGGTATCTAAAACCATTACATTGTGGCTGCAATGACAATATTTGTTACGATATATATAGTTAATACTTGGATTTGACATAGTAAGATGTTATCTGTTAGTGATAGAGGTGTCATTAGGAAAGGGAACTAGTTAGAACTTAGAGGAGACTGTTAAATATACAATTTACTCTTGTTGTGTTCCATTGTTCCACCCTACCAAAGTTCTATTTCTCTTTATTCAAGACTCTCTCAAGTATAATTTCTAAACTCCAACTTTCATTCTAACCATTTGTGTTTTAACATTTTATACAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACGAATACAATTTTGGAAAGTAGCTCAAAGAACAATGACGAACCACGGAACCAATATGTTGATGGAGAAGAATTGGGCAATTATAAGATAAATAAAATGGATATTAAAGTATTGCAAAGCAAATCTCAGAATAAGATCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTAGTTTCCTTACCATTCCATTAGGGTCCATTATAAAGACTTTAATACCCAACAATTTTCCTGGATGTGTTGGTAATTTGTACAACAGTGTGAAGAACTTGGATCCCGTGTCTGAGTTGCTCCATCCTTGTATAGCACCACACTTTGCTTGTCCAAAGCAACCCCTTGACATTCATCCTTTAGAATCCCCTACTTATTATTATGGTATTCATCACAATGATGATGATGATGATTATAATACAATAGAGGAAGTGATTTCTAATTCACATGTATCAATTGTTAATCGGATAAACGTGATTGAACTTGACCCAAGAGAAGGTGCTGTAGGATTTGTGAAGAGAGCAGCATTATATATGGTAGGAGATGATCTAGAAGTAAAACCACTTGCTGCTAATTCTTTCCTTTCATATCTGAAAGAATTAAGCCTTCCTCTTGATGATCTTGAAGTGACCGTGATTACCATTGATGAAAGGGAGGTAAGATGGCTATTATTTAATTAATTACTGAATTTATTACCTTTTTTCTGTTTTTTTAACCTCTTTGATTCCCTTTAATTGTGGTGTAGGCTTTGTGCTTCCTTCGAGCATTCTTCACATCAAAATTTACCCTGACTAGTGGACTTAGACGTTTTTTACCAGCTTTCAAATGGAAGAGAATTATCTATGAGTTTTAG
Protein sequence

>MS.gene70042.t1

MTSQQEQQEESKTFPLKITVDKQSNKVVFVEATKDFVETLFSFLSLPLGTIVRLLATTNNNDRQKLSESSPFFGNIKHLYQTVQNLNSDDVWNNPLCKQMLLHPRNSCESLCRKLYLNIDDSEPSSKFYVCDSCNKFTTFRNLDCTCGKPPNRQPKNLDSEGQGNHASNGVFDTESEQMFLVSDDLNIVPSSLLNSMQMLLESGISDSTQLEEVTQSIGKKEILNLLKYTLTSHEPLTNTILESSSKNNDEPRNQYVDGEELGNYKINKMDIKVLQSKSQNKIIIAEADGDFIDFIFSFLTIPLGSIIKTLIPNNFPGCVGNLYNSVKNLDPVSELLHPCIAPHFACPKQPLDIHPLESPTYYYGIHHNDDDDDYNTIEEVISNSHVSIVNRINVIELDPREGAVGFVKRAALYMVGDDLEVKPLAANSFLSYLKELSLPLDDLEVTVITIDEREALCFLRAFFTSKFTLTSGLRRFLPAFKWKRIIYEF