AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene89789

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89789.t1 MTR_4g017470 76.566 431 62 2 1 430 101 493 0.0 652
MS.gene89789.t1 MTR_4g017470 26.562 256 107 6 169 403 9 204 1.58e-11 66.2
MS.gene89789.t1 MTR_4g017410 74.419 430 82 3 1 426 98 503 0.0 632
MS.gene89789.t1 MTR_4g017410 27.451 255 144 9 171 403 8 243 3.83e-13 71.6
MS.gene89789.t1 MTR_4g017550 72.906 406 96 5 1 404 77 470 0.0 570
MS.gene89789.t1 MTR_4g017440 68.116 414 121 4 1 413 105 508 0.0 557
MS.gene89789.t1 MTR_4g017830 66.746 421 128 5 1 416 97 510 0.0 553
MS.gene89789.t1 MTR_4g019340 67.662 402 99 4 1 397 128 503 0.0 542
MS.gene89789.t1 MTR_4g017610 58.313 403 141 6 1 403 100 475 1.24e-155 449
MS.gene89789.t1 MTR_4g019310 40.326 429 218 14 1 415 95 499 1.83e-80 257
MS.gene89789.t1 MTR_4g019310 26.939 245 149 6 172 400 8 238 6.24e-12 67.8
MS.gene89789.t1 MTR_7g058710 33.333 432 242 13 1 413 79 483 7.16e-56 192
MS.gene89789.t1 MTR_7g058710 26.337 243 150 6 163 400 3 221 1.96e-14 75.5
MS.gene89789.t1 MTR_7g066340 31.354 421 217 11 1 407 91 453 1.03e-53 186
MS.gene89789.t1 MTR_7g066340 26.000 250 148 9 165 400 5 231 8.58e-14 73.6
MS.gene89789.t1 MTR_7g066570 32.584 445 247 13 1 426 93 503 8.59e-53 186
MS.gene89789.t1 MTR_7g066350 32.010 403 205 10 1 391 88 433 1.52e-51 180
MS.gene89789.t1 MTR_7g066350 25.547 274 157 9 170 428 7 248 5.46e-12 67.8
MS.gene89789.t1 MTR_7g066410 33.630 449 233 14 1 408 89 513 4.16e-49 175
MS.gene89789.t1 MTR_7g066430 33.871 434 246 12 1 416 83 493 6.75e-48 171
MS.gene89789.t1 MTR_7g066260 31.442 423 214 13 1 404 94 459 2.65e-47 169
MS.gene89789.t1 MTR_7g066260 29.796 245 131 9 172 400 15 234 2.78e-14 75.1
MS.gene89789.t1 MTR_7g066400 32.706 425 214 15 1 412 75 440 2.31e-46 166
MS.gene89789.t1 MTR_1g041315 31.280 422 196 16 1 391 93 451 2.45e-42 156
MS.gene89789.t1 MTR_7g062280 31.448 442 235 14 1 413 89 491 1.19e-41 154
MS.gene89789.t1 MTR_7g062280 26.316 247 145 9 173 400 11 239 3.12e-11 65.5
MS.gene89789.t1 MTR_7g063660 30.374 428 245 12 1 413 88 477 5.11e-40 149
MS.gene89789.t1 MTR_7g063660 36.000 125 69 3 171 288 8 128 2.87e-14 74.7
MS.gene89789.t1 MTR_7g012680 31.395 430 207 14 1 413 93 451 4.89e-39 146
MS.gene89789.t1 MTR_7g063900 31.429 420 251 13 1 408 89 483 1.07e-38 146
MS.gene89789.t1 MTR_7g063900 27.160 243 138 9 171 400 9 225 5.79e-13 70.9
MS.gene89789.t1 MTR_7g063880 29.812 426 239 15 1 413 90 468 2.91e-37 142
MS.gene89789.t1 MTR_7g063880 32.540 126 74 4 163 280 2 124 6.88e-11 64.3
MS.gene89789.t1 MTR_7g066280 27.251 422 252 12 1 403 1 386 4.71e-36 137
MS.gene89789.t1 MTR_7g062300 28.947 418 249 11 1 406 91 472 2.16e-35 136
MS.gene89789.t1 MTR_7g062300 26.908 249 149 8 163 400 3 229 1.51e-12 69.7
MS.gene89789.t1 MTR_7g066580 28.878 419 233 12 1 404 74 442 5.60e-34 132
MS.gene89789.t1 MTR_7g063840 29.040 427 247 14 1 413 89 473 6.96e-34 132
MS.gene89789.t1 MTR_7g063890 30.435 437 242 15 1 413 106 504 7.12e-34 132
MS.gene89789.t1 MTR_7g066360 31.731 416 210 16 1 404 93 446 1.03e-33 132
MS.gene89789.t1 MTR_7g063820 30.303 429 251 12 1 413 88 484 3.54e-33 130
MS.gene89789.t1 MTR_7g063830 29.508 427 216 14 1 413 89 444 8.74e-32 126
MS.gene89789.t1 MTR_7g058700 33.333 285 130 9 1 255 584 838 3.72e-31 127
MS.gene89789.t1 MTR_7g066310 26.988 415 170 10 1 403 86 379 6.96e-31 123
MS.gene89789.t1 MTR_3g065200 27.561 410 222 11 1 401 72 415 5.44e-29 118
MS.gene89789.t1 MTR_7g066290 33.922 283 152 9 1 263 94 361 1.56e-27 114
MS.gene89789.t1 MTR_3g007740 27.123 424 235 12 2 408 77 443 2.11e-27 114
MS.gene89789.t1 MTR_7g063920 27.208 419 227 12 1 413 90 436 1.21e-25 108
MS.gene89789.t1 MTR_7g063670 28.774 424 241 15 2 413 83 457 7.14e-25 106
MS.gene89789.t1 MTR_7g066680 30.037 273 159 7 123 386 37 286 9.07e-24 102
MS.gene89789.t1 MTR_7g066480 33.582 268 147 10 1 254 95 345 4.89e-23 100
MS.gene89789.t1 MTR_2g029400 25.990 404 197 11 1 395 93 403 3.94e-22 98.2
MS.gene89789.t1 MTR_4g019350 50.000 98 33 2 230 326 1 83 7.31e-22 89.7
MS.gene89789.t1 MTR_8g445860 31.818 242 122 8 177 402 19 233 1.29e-20 90.9
MS.gene89789.t1 MTR_7g063720 29.536 237 129 5 175 408 26 227 1.38e-18 85.1
MS.gene89789.t1 MTR_7g066490 27.190 331 202 11 1 321 95 396 1.74e-15 78.2
MS.gene89789.t1 MTR_1g115440 28.800 250 151 8 163 402 2 234 4.40e-15 75.1
MS.gene89789.t1 MTR_1g014060 33.796 216 104 7 202 413 210 390 6.90e-15 76.3
MS.gene89789.t1 MTR_1g014060 33.793 145 81 3 1 138 79 215 5.35e-11 64.3
MS.gene89789.t1 MTR_1g115450 50.000 74 34 2 177 248 13 85 3.36e-14 70.1
MS.gene89789.t1 MTR_7g100420 27.740 292 168 7 123 403 134 393 7.87e-14 73.2
MS.gene89789.t1 MTR_8g023240 24.885 217 141 5 59 260 159 368 1.14e-12 70.1
MS.gene89789.t1 MTR_4g019240 61.538 52 20 0 365 416 112 163 8.39e-12 64.3
MS.gene89789.t1 MTR_1g115455 41.250 80 43 2 172 248 9 87 1.53e-11 64.3
MS.gene89789.t1 MTR_0150s0030 38.272 81 47 1 172 249 8 88 2.74e-11 63.2
MS.gene89789.t1 MTR_0150s0010 39.535 86 50 1 165 248 3 88 4.14e-11 63.2
MS.gene89789.t1 MTR_4g019330 44.578 83 44 2 333 413 106 188 4.65e-11 62.0
MS.gene89789.t1 MTR_8g445910 39.506 81 46 1 172 249 8 88 7.17e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene89789.t1 AT5G01130 29.639 415 239 12 1 413 88 451 8.13e-41 151
MS.gene89789.t1 AT5G01120 27.918 437 282 12 1 423 88 505 3.61e-40 150
MS.gene89789.t1 AT5G43240 29.439 428 251 12 1 403 88 489 4.53e-40 150
MS.gene89789.t1 AT5G43240 29.439 428 251 12 1 403 88 489 4.53e-40 150
MS.gene89789.t1 AT5G43240 29.439 428 251 12 1 403 88 489 4.53e-40 150
MS.gene89789.t1 AT5G01140 28.741 421 258 12 1 413 72 458 1.07e-39 148
MS.gene89789.t1 AT3G09140 29.062 437 265 14 1 415 88 501 2.80e-34 134
MS.gene89789.t1 AT3G09140 27.615 239 161 7 171 400 8 243 7.54e-15 77.0
MS.gene89789.t1 AT5G01150 27.703 444 255 13 1 418 88 491 7.95e-34 132
MS.gene89789.t1 AT5G43240 28.895 353 213 7 65 403 17 345 6.63e-32 125
MS.gene89789.t1 AT3G09140 27.312 465 265 15 1 415 88 529 1.89e-29 120
MS.gene89789.t1 AT3G09140 25.292 257 180 7 171 418 8 261 1.10e-11 67.0
MS.gene89789.t1 AT5G37320 27.040 429 255 15 1 408 89 480 2.95e-28 116
MS.gene89789.t1 AT3G09110 32.917 240 143 7 2 233 89 318 1.20e-27 112
MS.gene89789.t1 AT3G09120 31.933 238 142 8 1 226 84 313 2.99e-24 102
MS.gene89789.t1 AT3G09120 25.833 240 154 7 171 401 5 229 6.14e-14 72.8

Find 94 sgRNAs with CRISPR-Local

Find 111 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAATAGACAGCCTAAGAATT+TGG 0.219049 4.4:-83456624 MS.gene89789:CDS
ATAGGTGGTGGCTGCATTTC+GGG 0.227200 4.4:+83455850 None:intergenic
AGGTGGGCTGTCTTTGTTCT+TGG 0.232574 4.4:+83456200 None:intergenic
CCACCTATTACATATTATTA+TGG 0.255078 4.4:-83455835 MS.gene89789:CDS
CAACGGATCACAAGTTGATA+TGG 0.270644 4.4:-83455659 MS.gene89789:CDS
AAAAGAGTTGTTTCTTGTTT+AGG 0.272026 4.4:+83455332 None:intergenic
AGAAAATGGATCCTTGTTTA+TGG 0.286508 4.4:-83456555 MS.gene89789:CDS
CACCGGTGACATCAGGAAAA+TGG 0.299634 4.4:-83456142 MS.gene89789:CDS
CCATCAGAATCCAAATTCTT+AGG 0.304525 4.4:+83456614 None:intergenic
TAAACAAGAAACAACTCTTT+TGG 0.309535 4.4:-83455330 MS.gene89789:CDS
AACAAGAGAAGTAAAGATAG+AGG 0.310667 4.4:-83455755 MS.gene89789:CDS
AGCTTCCTTACGTTGCCATT+AGG 0.316633 4.4:-83456039 MS.gene89789:CDS
CTCTAACTGCTGATGCAAAT+TGG 0.325970 4.4:+83456175 None:intergenic
GTAAAGAGAGCAGCATTATA+TGG 0.327171 4.4:-83455631 MS.gene89789:CDS
GTACCATAATAATATGTAAT+AGG 0.339119 4.4:+83455832 None:intergenic
GCTTCCTTACGTTGCCATTA+GGG 0.349941 4.4:-83456038 MS.gene89789:CDS
TTAATTCTTTCAAATATAGA+AGG 0.350593 4.4:+83455563 None:intergenic
AATAGGTGGTGGCTGCATTT+CGG 0.354171 4.4:+83455849 None:intergenic
GGCGTCTTTATGACAGAAAA+TGG 0.355556 4.4:-83456569 MS.gene89789:CDS
TGTGATCCGTTGAGGGATCT+TGG 0.355842 4.4:+83455670 None:intergenic
AAGCGATTCACATGGATTCC+TGG 0.362784 4.4:+83456766 None:intergenic
GACTTCCATGCAGCTATTGA+TGG 0.367692 4.4:-83456495 MS.gene89789:CDS
CATGCAGCTATTGATGGAAC+TGG 0.379788 4.4:-83456489 MS.gene89789:CDS
GTGTAGGCTTTGAGCTTCCT+TGG 0.380217 4.4:-83455413 MS.gene89789:intron
CACACACAACATTGGCAAAC+AGG 0.390115 4.4:-83456435 MS.gene89789:CDS
AGCAAAATTCTTAACTGCAT+TGG 0.390278 4.4:-83455695 MS.gene89789:CDS
CACAGGTACAGTGAAGGTTT+CGG 0.392605 4.4:+83456657 None:intergenic
GAGAGGCTCATGGGAGGTTA+AGG 0.396809 4.4:+83456248 None:intergenic
GCTACTTCTCAAAATTGTAT+TGG 0.405537 4.4:+83456224 None:intergenic
CTTCAAGATCGTCAAGAGAA+AGG 0.418851 4.4:+83455539 None:intergenic
CTGCATTTCGGGAATGTTGA+GGG 0.428907 4.4:+83455861 None:intergenic
CTATATATAAACTCTTGCTT+TGG 0.439118 4.4:+83455802 None:intergenic
ATTCTTAGGCTGTCTATTAG+TGG 0.442822 4.4:+83456628 None:intergenic
AGATATACAACTGACCCTAA+TGG 0.442910 4.4:+83456024 None:intergenic
GGGAGGTTAAGGAATAATTC+AGG 0.442965 4.4:+83456259 None:intergenic
GTGATCCGTTGAGGGATCTT+GGG 0.444569 4.4:+83455671 None:intergenic
TTCTTAGGCTGTCTATTAGT+GGG 0.450651 4.4:+83456629 None:intergenic
CTTGTTTAGGCACACTCAAA+AGG 0.455083 4.4:+83455345 None:intergenic
CGTCTTGCACCGGTGACATC+AGG 0.455219 4.4:-83456149 MS.gene89789:CDS
TGGAGAACTTGGACTCTTCA+TGG 0.456560 4.4:-83455951 MS.gene89789:CDS
CGCACTGTGTTACTCAATCC+TGG 0.466642 4.4:-83455916 MS.gene89789:CDS
CCTGGTGTAGCACACCAGTT+TGG 0.470247 4.4:-83455898 MS.gene89789:CDS
GTACAAGAGTGTGGAGAACT+TGG 0.471755 4.4:-83455962 MS.gene89789:CDS
TTTCGGGAATGTTGAGGGGC+TGG 0.473505 4.4:+83455866 None:intergenic
GCTGCATTTCGGGAATGTTG+AGG 0.481946 4.4:+83455860 None:intergenic
ATTACATATTATTATGGTAC+AGG 0.484988 4.4:-83455829 MS.gene89789:CDS
AGCGATTCACATGGATTCCT+GGG 0.485112 4.4:+83456767 None:intergenic
AGAGCAGCATTATATGGTAT+AGG 0.490389 4.4:-83455625 MS.gene89789:CDS
GCTGGTTTCGACAACCAAAC+TGG 0.492728 4.4:+83455884 None:intergenic
GAAGTCAACAAAATCTCCAT+TGG 0.498206 4.4:+83456068 None:intergenic
AGGAAAGAATTAGCAGAGAG+TGG 0.503286 4.4:+83455583 None:intergenic
ACTGCTGATGCAAATTGGTT+AGG 0.504158 4.4:+83456180 None:intergenic
CTCAAAATTGTATTGGTGAG+AGG 0.509830 4.4:+83456231 None:intergenic
AACGGATCACAAGTTGATAT+GGG 0.520381 4.4:-83455658 MS.gene89789:CDS
TTCAGACTTGACTCAAATAG+AGG 0.521757 4.4:-83456462 MS.gene89789:CDS
ATGGAAGTCACAGAGGAACT+TGG 0.523683 4.4:+83456509 None:intergenic
ATCAACTTGTGATCCGTTGA+GGG 0.526068 4.4:+83455663 None:intergenic
ATTTGGATTCTGATGGTCAA+GGG 0.529572 4.4:-83456607 MS.gene89789:CDS
CCTAAGAATTTGGATTCTGA+TGG 0.538168 4.4:-83456614 MS.gene89789:CDS
TTGTATTGGTGAGAGGCTCA+TGG 0.539351 4.4:+83456238 None:intergenic
ATGCAGCTATTGATGGAACT+GGG 0.542926 4.4:-83456488 MS.gene89789:CDS
TAATAATATGTAATAGGTGG+TGG 0.544263 4.4:+83455838 None:intergenic
ACAAGAGAAGTAAAGATAGA+GGG 0.556606 4.4:-83455754 MS.gene89789:CDS
GACAAATGTTGCTTCATCCC+AGG 0.556708 4.4:-83456784 None:intergenic
CCAAACTGGTGTGCTACACC+AGG 0.558658 4.4:+83455898 None:intergenic
GTCTTTAAGTGCACTAGTCA+AGG 0.567139 4.4:+83455367 None:intergenic
TCTTGACGATCTTGAAGCGA+AGG 0.567610 4.4:-83455533 MS.gene89789:CDS
ATAATCTTTGCAGAAACCAA+TGG 0.568343 4.4:-83456084 MS.gene89789:CDS
CTGATGCAAATTGGTTAGGT+GGG 0.568436 4.4:+83456184 None:intergenic
TGTATTGGTGAGAGGCTCAT+GGG 0.577296 4.4:+83456239 None:intergenic
GCTGATGCAAATTGGTTAGG+TGG 0.578014 4.4:+83456183 None:intergenic
TGCATTTCGGGAATGTTGAG+GGG 0.579499 4.4:+83455862 None:intergenic
CGAAACCTTCACTGTACCTG+TGG 0.582669 4.4:-83456656 MS.gene89789:CDS
AGAGAAGTAAAGATAGAGGG+AGG 0.592777 4.4:-83455751 MS.gene89789:CDS
CAAGGGAATAATGCACAAGA+TGG 0.595731 4.4:-83456590 MS.gene89789:CDS
TATCAACTTGTGATCCGTTG+AGG 0.597413 4.4:+83455662 None:intergenic
GAAGCGAAGGTGATGAGCAT+CGG 0.608043 4.4:-83455520 MS.gene89789:CDS
GTTAGAGCAACGTCTTGCAC+CGG 0.611909 4.4:-83456159 MS.gene89789:CDS
CCATAATAATATGTAATAGG+TGG 0.620194 4.4:+83455835 None:intergenic
GAGGAAGTCACACACAACAT+TGG 0.621785 4.4:-83456443 MS.gene89789:CDS
AATTTGGATTCTGATGGTCA+AGG 0.623814 4.4:-83456608 MS.gene89789:CDS
CTGACCCTAATGGCAACGTA+AGG 0.626966 4.4:+83456034 None:intergenic
GATGAGCATCGGTGAAGCTG+AGG 0.627852 4.4:-83455509 MS.gene89789:intron
CAGTTCCATCAATAGCTGCA+TGG 0.629483 4.4:+83456490 None:intergenic
ATCATCAGACACCATAAACA+AGG 0.642521 4.4:+83456544 None:intergenic
TTGATGTCAAGAATGCTCCA+AGG 0.669048 4.4:+83455396 None:intergenic
TGCAGCTATTGATGGAACTG+GGG 0.677739 4.4:-83456487 MS.gene89789:CDS
ACACAACATTGGCAAACAGG+AGG 0.681241 4.4:-83456432 MS.gene89789:intron
TTCATGCAAAGCGATTCACA+TGG 0.692991 4.4:+83456758 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.696549 4.4:-83455971 MS.gene89789:CDS
ATTGGTGAGAGGCTCATGGG+AGG 0.700980 4.4:+83456242 None:intergenic
GGAGTGATTTCTAAGTCACA+CGG 0.701071 4.4:-83455730 MS.gene89789:CDS
TTGGACCCAAGATCCCTCAA+CGG 0.706241 4.4:-83455676 MS.gene89789:CDS
TAGCTGCATGGAAGTCACAG+AGG 0.730672 4.4:+83456502 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCTTTCAAATATAGA+AGG + chr4.4:83456508-83456527 None:intergenic 15.0%
!! AAAAATAGATGAATTACAGA+AGG + chr4.4:83455683-83455702 None:intergenic 20.0%
!! ATTACATATTATTATGGTAC+AGG - chr4.4:83456239-83456258 MS.gene89789:CDS 20.0%
!! CCAAAATGTTAAAACATAAA+TGG + chr4.4:83455774-83455793 None:intergenic 20.0%
!! GTACCATAATAATATGTAAT+AGG + chr4.4:83456239-83456258 None:intergenic 20.0%
!!! AACATAAAAAAAGTTGATTC+TGG + chr4.4:83456775-83456794 None:intergenic 20.0%
!!! CCATTTATGTTTTAACATTT+TGG - chr4.4:83455771-83455790 MS.gene89789:CDS 20.0%
! CACACAAAAAATTTACTTGA+TGG + chr4.4:83455364-83455383 None:intergenic 25.0%
! CCACCTATTACATATTATTA+TGG - chr4.4:83456233-83456252 MS.gene89789:CDS 25.0%
! CCATAATAATATGTAATAGG+TGG + chr4.4:83456236-83456255 None:intergenic 25.0%
! CTATATATAAACTCTTGCTT+TGG + chr4.4:83456269-83456288 None:intergenic 25.0%
! TAATAATATGTAATAGGTGG+TGG + chr4.4:83456233-83456252 None:intergenic 25.0%
!! TAAACAAGAAACAACTCTTT+TGG - chr4.4:83456738-83456757 MS.gene89789:CDS 25.0%
!!! AAAAAAGTTGATTCTGGTTT+AGG + chr4.4:83456769-83456788 None:intergenic 25.0%
!!! AAAAGAGTTGTTTCTTGTTT+AGG + chr4.4:83456739-83456758 None:intergenic 25.0%
AAAGCCTACACAACAATTAA+AGG + chr4.4:83456646-83456665 None:intergenic 30.0%
AACAAGAGAAGTAAAGATAG+AGG - chr4.4:83456313-83456332 MS.gene89789:intron 30.0%
AAGCCTACACAACAATTAAA+GGG + chr4.4:83456645-83456664 None:intergenic 30.0%
ACAAGAGAAGTAAAGATAGA+GGG - chr4.4:83456314-83456333 MS.gene89789:intron 30.0%
ACAATTAAAGGGAATCAAAG+CGG + chr4.4:83456634-83456653 None:intergenic 30.0%
AGCAAAATTCTTAACTGCAT+TGG - chr4.4:83456373-83456392 MS.gene89789:intron 30.0%
ATAATCTTTGCAGAAACCAA+TGG - chr4.4:83455984-83456003 MS.gene89789:CDS 30.0%
ATTCCCTTTAATTGTTGTGT+AGG - chr4.4:83456639-83456658 MS.gene89789:CDS 30.0%
GAATTACAGAAGGAAATACA+CGG + chr4.4:83455673-83455692 None:intergenic 30.0%
GCTACTTCTCAAAATTGTAT+TGG + chr4.4:83455847-83455866 None:intergenic 30.0%
TAATAGACAGCCTAAGAATT+TGG - chr4.4:83455444-83455463 MS.gene89789:intron 30.0%
! AGAAAATGGATCCTTGTTTA+TGG - chr4.4:83455513-83455532 MS.gene89789:CDS 30.0%
! TTAGGAGCTAATAATTTTGC+TGG - chr4.4:83456068-83456087 MS.gene89789:CDS 30.0%
AATTTGGATTCTGATGGTCA+AGG - chr4.4:83455460-83455479 MS.gene89789:intron 35.0%
AGAGCAGCATTATATGGTAT+AGG - chr4.4:83456443-83456462 MS.gene89789:CDS 35.0%
AGATATACAACTGACCCTAA+TGG + chr4.4:83456047-83456066 None:intergenic 35.0%
ATCATCAGACACCATAAACA+AGG + chr4.4:83455527-83455546 None:intergenic 35.0%
ATTCTTAGGCTGTCTATTAG+TGG + chr4.4:83455443-83455462 None:intergenic 35.0%
ATTTGGATTCTGATGGTCAA+GGG - chr4.4:83455461-83455480 MS.gene89789:intron 35.0%
CCATCAGAATCCAAATTCTT+AGG + chr4.4:83455457-83455476 None:intergenic 35.0%
CCTAAGAATTTGGATTCTGA+TGG - chr4.4:83455454-83455473 MS.gene89789:intron 35.0%
GAAGTCAACAAAATCTCCAT+TGG + chr4.4:83456003-83456022 None:intergenic 35.0%
GTAAAGAGAGCAGCATTATA+TGG - chr4.4:83456437-83456456 MS.gene89789:CDS 35.0%
TTCAGACTTGACTCAAATAG+AGG - chr4.4:83455606-83455625 MS.gene89789:CDS 35.0%
TTCTTAGGCTGTCTATTAGT+GGG + chr4.4:83455442-83455461 None:intergenic 35.0%
! AATAATTTTGCTGGATGTGC+TGG - chr4.4:83456077-83456096 MS.gene89789:CDS 35.0%
! CTCAAAATTGTATTGGTGAG+AGG + chr4.4:83455840-83455859 None:intergenic 35.0%
! GGGTCAGTTGTATATCTTTT+AGG - chr4.4:83456050-83456069 MS.gene89789:CDS 35.0%
! TGGTAATTTGTACAAGAGTG+TGG - chr4.4:83456097-83456116 MS.gene89789:CDS 35.0%
!! AACGGATCACAAGTTGATAT+GGG - chr4.4:83456410-83456429 MS.gene89789:intron 35.0%
ACTGCTGATGCAAATTGGTT+AGG + chr4.4:83455891-83455910 None:intergenic 40.0%
AGAGAAGTAAAGATAGAGGG+AGG - chr4.4:83456317-83456336 MS.gene89789:intron 40.0%
AGGAAAGAATTAGCAGAGAG+TGG + chr4.4:83456488-83456507 None:intergenic 40.0%
ATCAACTTGTGATCCGTTGA+GGG + chr4.4:83456408-83456427 None:intergenic 40.0%
ATGCAGCTATTGATGGAACT+GGG - chr4.4:83455580-83455599 MS.gene89789:CDS 40.0%
CAAGGGAATAATGCACAAGA+TGG - chr4.4:83455478-83455497 MS.gene89789:intron 40.0%
CTCTAACTGCTGATGCAAAT+TGG + chr4.4:83455896-83455915 None:intergenic 40.0%
CTGATGCAAATTGGTTAGGT+GGG + chr4.4:83455887-83455906 None:intergenic 40.0%
CTTCAAGATCGTCAAGAGAA+AGG + chr4.4:83456532-83456551 None:intergenic 40.0%
CTTGTTTAGGCACACTCAAA+AGG + chr4.4:83456726-83456745 None:intergenic 40.0%
GGAGTGATTTCTAAGTCACA+CGG - chr4.4:83456338-83456357 MS.gene89789:intron 40.0%
GGCGTCTTTATGACAGAAAA+TGG - chr4.4:83455499-83455518 MS.gene89789:intron 40.0%
GGGAGGTTAAGGAATAATTC+AGG + chr4.4:83455812-83455831 None:intergenic 40.0%
GTCTTTAAGTGCACTAGTCA+AGG + chr4.4:83456704-83456723 None:intergenic 40.0%
TATCAACTTGTGATCCGTTG+AGG + chr4.4:83456409-83456428 None:intergenic 40.0%
TTCATGCAAAGCGATTCACA+TGG + chr4.4:83455313-83455332 None:intergenic 40.0%
TTGATGTCAAGAATGCTCCA+AGG + chr4.4:83456675-83456694 None:intergenic 40.0%
! ATCCATTTTCCTGATGTCAC+CGG + chr4.4:83455931-83455950 None:intergenic 40.0%
!! CAACGGATCACAAGTTGATA+TGG - chr4.4:83456409-83456428 MS.gene89789:intron 40.0%
!!! TCTATTAGTGGGTTTTCCAC+AGG + chr4.4:83455431-83455450 None:intergenic 40.0%
AAGCGATTCACATGGATTCC+TGG + chr4.4:83455305-83455324 None:intergenic 45.0%
ACACAACATTGGCAAACAGG+AGG - chr4.4:83455636-83455655 MS.gene89789:CDS 45.0%
AGCGATTCACATGGATTCCT+GGG + chr4.4:83455304-83455323 None:intergenic 45.0%
AGCTTCCTTACGTTGCCATT+AGG - chr4.4:83456029-83456048 MS.gene89789:CDS 45.0%
ATGGAAGTCACAGAGGAACT+TGG + chr4.4:83455562-83455581 None:intergenic 45.0%
CACACACAACATTGGCAAAC+AGG - chr4.4:83455633-83455652 MS.gene89789:CDS 45.0%
CACAGGTACAGTGAAGGTTT+CGG + chr4.4:83455414-83455433 None:intergenic 45.0%
CAGTTCCATCAATAGCTGCA+TGG + chr4.4:83455581-83455600 None:intergenic 45.0%
CATGCAGCTATTGATGGAAC+TGG - chr4.4:83455579-83455598 MS.gene89789:CDS 45.0%
CATTGGCAAACAGGAGGTAA+TGG - chr4.4:83455642-83455661 MS.gene89789:CDS 45.0%
CTGCATTTCGGGAATGTTGA+GGG + chr4.4:83456210-83456229 None:intergenic 45.0%
GACTTCCATGCAGCTATTGA+TGG - chr4.4:83455573-83455592 MS.gene89789:CDS 45.0%
GAGGAAGTCACACACAACAT+TGG - chr4.4:83455625-83455644 MS.gene89789:CDS 45.0%
GCTGATGCAAATTGGTTAGG+TGG + chr4.4:83455888-83455907 None:intergenic 45.0%
GCTTCCTTACGTTGCCATTA+GGG - chr4.4:83456030-83456049 MS.gene89789:CDS 45.0%
TCTTGACGATCTTGAAGCGA+AGG - chr4.4:83456535-83456554 MS.gene89789:CDS 45.0%
TGCAGCTATTGATGGAACTG+GGG - chr4.4:83455581-83455600 MS.gene89789:CDS 45.0%
TGCATTTCGGGAATGTTGAG+GGG + chr4.4:83456209-83456228 None:intergenic 45.0%
TGGAGAACTTGGACTCTTCA+TGG - chr4.4:83456117-83456136 MS.gene89789:CDS 45.0%
! AATAGGTGGTGGCTGCATTT+CGG + chr4.4:83456222-83456241 None:intergenic 45.0%
! GTACAAGAGTGTGGAGAACT+TGG - chr4.4:83456106-83456125 MS.gene89789:CDS 45.0%
! GTTTTCCACAGGTACAGTGA+AGG + chr4.4:83455420-83455439 None:intergenic 45.0%
! TGTATTGGTGAGAGGCTCAT+GGG + chr4.4:83455832-83455851 None:intergenic 45.0%
! TTGTATTGGTGAGAGGCTCA+TGG + chr4.4:83455833-83455852 None:intergenic 45.0%
CACCGGTGACATCAGGAAAA+TGG - chr4.4:83455926-83455945 MS.gene89789:CDS 50.0%
CGAAACCTTCACTGTACCTG+TGG - chr4.4:83455412-83455431 MS.gene89789:intron 50.0%
CTGACCCTAATGGCAACGTA+AGG + chr4.4:83456037-83456056 None:intergenic 50.0%
GAAGCGAAGGTGATGAGCAT+CGG - chr4.4:83456548-83456567 MS.gene89789:CDS 50.0%
GCTGCATTTCGGGAATGTTG+AGG + chr4.4:83456211-83456230 None:intergenic 50.0%
GTGATCCGTTGAGGGATCTT+GGG + chr4.4:83456400-83456419 None:intergenic 50.0%
GTTAGAGCAACGTCTTGCAC+CGG - chr4.4:83455909-83455928 MS.gene89789:CDS 50.0%
TAGCTGCATGGAAGTCACAG+AGG + chr4.4:83455569-83455588 None:intergenic 50.0%
TGTGATCCGTTGAGGGATCT+TGG + chr4.4:83456401-83456420 None:intergenic 50.0%
TTGGACCCAAGATCCCTCAA+CGG - chr4.4:83456392-83456411 MS.gene89789:intron 50.0%
! ATAGGTGGTGGCTGCATTTC+GGG + chr4.4:83456221-83456240 None:intergenic 50.0%
! CGCACTGTGTTACTCAATCC+TGG - chr4.4:83456152-83456171 MS.gene89789:CDS 50.0%
! GCTGGTTTCGACAACCAAAC+TGG + chr4.4:83456187-83456206 None:intergenic 50.0%
!! AGGTGGGCTGTCTTTGTTCT+TGG + chr4.4:83455871-83455890 None:intergenic 50.0%
!! GTGTAGGCTTTGAGCTTCCT+TGG - chr4.4:83456655-83456674 MS.gene89789:CDS 50.0%
ATTGGTGAGAGGCTCATGGG+AGG + chr4.4:83455829-83455848 None:intergenic 55.0%
CCAAACTGGTGTGCTACACC+AGG + chr4.4:83456173-83456192 None:intergenic 55.0%
CCTGGTGTAGCACACCAGTT+TGG - chr4.4:83456170-83456189 MS.gene89789:CDS 55.0%
GAGAGGCTCATGGGAGGTTA+AGG + chr4.4:83455823-83455842 None:intergenic 55.0%
TTTCGGGAATGTTGAGGGGC+TGG + chr4.4:83456205-83456224 None:intergenic 55.0%
!! GATGAGCATCGGTGAAGCTG+AGG - chr4.4:83456559-83456578 MS.gene89789:CDS 55.0%
! CGTCTTGCACCGGTGACATC+AGG - chr4.4:83455919-83455938 MS.gene89789:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 83455289 83456801 83455289 ID=MS.gene89789
chr4.4 mRNA 83455289 83456801 83455289 ID=MS.gene89789.t1;Parent=MS.gene89789
chr4.4 exon 83456433 83456801 83456433 ID=MS.gene89789.t1.exon1;Parent=MS.gene89789.t1
chr4.4 CDS 83456433 83456801 83456433 ID=cds.MS.gene89789.t1;Parent=MS.gene89789.t1
chr4.4 exon 83455510 83456292 83455510 ID=MS.gene89789.t1.exon2;Parent=MS.gene89789.t1
chr4.4 CDS 83455510 83456292 83455510 ID=cds.MS.gene89789.t1;Parent=MS.gene89789.t1
chr4.4 exon 83455289 83455429 83455289 ID=MS.gene89789.t1.exon3;Parent=MS.gene89789.t1
chr4.4 CDS 83455289 83455429 83455289 ID=cds.MS.gene89789.t1;Parent=MS.gene89789.t1
Gene Sequence

>MS.gene89789

ATGTTGCTTCATCCCAGGAATCCATGTGAATCGCTTTGCATGAAGCTGTTCTTGAATGTTGATGACACTGAGCCATCAAGTAAATTTTTTGTGTGTGATTCTTGTAACACGTTTACTACTTTCCGAAACCTTCACTGTACCTGTGGAAAACCCACTAATAGACAGCCTAAGAATTTGGATTCTGATGGTCAAGGGAATAATGCACAAGATGGCGTCTTTATGACAGAAAATGGATCCTTGTTTATGGTGTCTGATGATTTGAAGATCGTGCCAAGTTCCTCTGTGACTTCCATGCAGCTATTGATGGAACTGGGGTATTCAGACTTGACTCAAATAGAGGAAGTCACACACAACATTGGCAAACAGGAGGTAATGGTGTTACCGTGTATTTCCTTCTGTAATTCATCTATTTTTAAACAATATTATAATTTCTTCTCTCTAAATTCGAGTTTGTATCAGTCTTTATTTAACTGTTATGCTAACCATTTATGTTTTAACATTTTGGATAGATATTGAAACTCCTGAATTATTCCTTAACCTCCCATGAGCCTCTCACCAATACAATTTTGAGAAGTAGCTCCAAGAACAAAGACAGCCCACCTAACCAATTTGCATCAGCAGTTAGAGCAACGTCTTGCACCGGTGACATCAGGAAAATGGATGTTAAAGTAATTCAATGTAAATCTCAGAAAAATATAATCTTTGCAGAAACCAATGGAGATTTTGTTGACTTCATTTTTAGCTTCCTTACGTTGCCATTAGGGTCAGTTGTATATCTTTTAGGAGCTAATAATTTTGCTGGATGTGCTGGTAATTTGTACAAGAGTGTGGAGAACTTGGACTCTTCATGGTGTATTGACTCACGCACTGTGTTACTCAATCCTGGTGTAGCACACCAGTTTGGTTGTCGAAACCAGCCCCTCAACATTCCCGAAATGCAGCCACCACCTATTACATATTATTATGGTACAGGAACACCAAAGCAAGAGTTTATATATAGTTATCATAGACGTGATTATTATGAAACAAGAGAAGTAAAGATAGAGGGAGGAGTGATTTCTAAGTCACACGGATCAATTTATAAAGCAAAATTCTTAACTGCATTGGACCCAAGATCCCTCAACGGATCACAAGTTGATATGGGATTTGTAAAGAGAGCAGCATTATATGGTATAGGAAATGATCTAAGAGTAAAACCACTCTCTGCTAATTCTTTCCTTCTATATTTGAAAGAATTAAACCTTTCTCTTGACGATCTTGAAGCGAAGGTGATGAGCATCGGTGAAGCTGAGGTAATGTGCTATTATTTATCACTAAATATTTTCTTTTTTTGTTATTTCAGCCGCTTTGATTCCCTTTAATTGTTGTGTAGGCTTTGAGCTTCCTTGGAGCATTCTTGACATCAAGATTTACCTTGACTAGTGCACTTAAAGACCTTTTGAGTGTGCCTAAACAAGAAACAACTCTTTTGGATATTCCTAAACCAGAATCAACTTTTTTTATGTTAATTTGA
Protein sequence

>MS.gene89789.t1

MLLHPRNPCESLCMKLFLNVDDTEPSSKFFVCDSCNTFTTFRNLHCTCGKPTNRQPKNLDSDGQGNNAQDGVFMTENGSLFMVSDDLKIVPSSSVTSMQLLMELGYSDLTQIEEVTHNIGKQEILKLLNYSLTSHEPLTNTILRSSSKNKDSPPNQFASAVRATSCTGDIRKMDVKVIQCKSQKNIIFAETNGDFVDFIFSFLTLPLGSVVYLLGANNFAGCAGNLYKSVENLDSSWCIDSRTVLLNPGVAHQFGCRNQPLNIPEMQPPPITYYYGTGTPKQEFIYSYHRRDYYETREVKIEGGVISKSHGSIYKAKFLTALDPRSLNGSQVDMGFVKRAALYGIGNDLRVKPLSANSFLLYLKELNLSLDDLEAKVMSIGEAEALSFLGAFLTSRFTLTSALKDLLSVPKQETTLLDIPKPESTFFMLI