AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene048067

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048067.t1 MTR_4g017830 66.984 315 99 3 15 324 213 527 9.62e-138 401
MS.gene048067.t1 MTR_4g017830 27.311 238 145 6 71 294 15 238 9.36e-14 72.4
MS.gene048067.t1 MTR_4g019340 71.739 276 53 3 15 287 249 502 4.89e-125 368
MS.gene048067.t1 MTR_4g019340 28.340 247 141 7 71 294 41 274 8.63e-14 72.4
MS.gene048067.t1 MTR_4g017440 64.138 290 100 4 15 302 219 506 8.10e-116 344
MS.gene048067.t1 MTR_4g017440 27.755 245 139 8 69 292 15 242 2.34e-13 70.9
MS.gene048067.t1 MTR_4g017470 61.462 301 108 5 15 307 179 479 4.49e-113 337
MS.gene048067.t1 MTR_4g017470 27.309 249 109 8 64 294 10 204 1.10e-11 65.9
MS.gene048067.t1 MTR_4g017550 61.538 286 95 7 20 298 196 473 1.18e-100 304
MS.gene048067.t1 MTR_4g017410 54.485 301 104 6 15 307 218 493 3.67e-90 278
MS.gene048067.t1 MTR_4g017410 26.749 243 144 6 71 292 12 241 2.76e-16 79.7
MS.gene048067.t1 MTR_4g017610 53.818 275 106 6 20 294 222 475 4.87e-84 262
MS.gene048067.t1 MTR_4g017610 29.752 242 136 9 71 294 16 241 2.90e-13 70.5
MS.gene048067.t1 MTR_4g019310 43.525 278 141 6 20 294 222 486 1.99e-60 201
MS.gene048067.t1 MTR_4g019310 30.081 246 147 7 62 291 2 238 1.51e-18 86.7
MS.gene048067.t1 MTR_4g019240 95.833 72 3 0 257 328 113 184 2.91e-38 134
MS.gene048067.t1 MTR_7g066340 32.476 311 155 7 20 326 215 474 9.37e-36 135
MS.gene048067.t1 MTR_7g066340 28.085 235 143 7 68 291 12 231 9.36e-18 84.0
MS.gene048067.t1 MTR_7g066310 35.656 244 113 6 68 309 193 394 1.82e-35 133
MS.gene048067.t1 MTR_7g066400 32.857 280 146 4 21 294 190 433 6.00e-34 129
MS.gene048067.t1 MTR_7g066430 34.211 304 177 7 9 301 197 488 8.17e-34 130
MS.gene048067.t1 MTR_7g066410 34.722 288 170 5 21 298 233 512 1.23e-33 129
MS.gene048067.t1 MTR_7g066570 35.395 291 164 7 21 302 209 484 3.47e-33 129
MS.gene048067.t1 MTR_7g062280 33.564 289 168 7 20 304 223 491 2.92e-32 125
MS.gene048067.t1 MTR_7g062280 32.906 234 141 9 69 291 11 239 4.52e-15 76.3
MS.gene048067.t1 MTR_7g066350 33.452 281 136 5 20 294 210 445 5.37e-32 124
MS.gene048067.t1 MTR_7g066350 29.435 248 149 8 68 307 9 238 4.68e-17 82.0
MS.gene048067.t1 MTR_7g066260 33.333 312 162 7 20 325 218 489 6.88e-32 124
MS.gene048067.t1 MTR_7g066260 29.237 236 139 7 68 291 15 234 1.58e-14 74.3
MS.gene048067.t1 MTR_7g058710 30.743 296 177 5 20 304 205 483 1.13e-31 124
MS.gene048067.t1 MTR_7g058710 26.383 235 137 8 68 291 12 221 1.27e-14 74.7
MS.gene048067.t1 MTR_7g066280 33.721 258 143 6 71 309 153 401 1.46e-31 122
MS.gene048067.t1 MTR_1g041315 34.559 272 125 8 19 282 225 451 2.89e-31 122
MS.gene048067.t1 MTR_7g012680 34.495 287 143 7 20 297 194 444 6.26e-30 119
MS.gene048067.t1 MTR_7g012680 26.506 249 132 8 56 292 2 211 5.56e-14 72.8
MS.gene048067.t1 MTR_7g066580 32.168 286 179 6 14 291 160 438 1.12e-29 118
MS.gene048067.t1 MTR_7g063720 32.766 235 125 4 63 294 18 222 3.16e-29 112
MS.gene048067.t1 MTR_7g062300 33.096 281 158 8 20 294 213 469 7.60e-29 115
MS.gene048067.t1 MTR_7g062300 30.638 235 133 9 69 291 13 229 3.05e-14 73.6
MS.gene048067.t1 MTR_7g063920 30.208 288 173 6 20 304 174 436 8.91e-28 112
MS.gene048067.t1 MTR_7g066680 31.579 323 150 10 19 294 36 334 1.11e-27 110
MS.gene048067.t1 MTR_7g063840 30.662 287 177 6 20 304 207 473 1.83e-27 112
MS.gene048067.t1 MTR_7g063840 28.511 235 134 10 69 291 11 223 5.52e-11 63.5
MS.gene048067.t1 MTR_7g066360 32.886 298 145 8 35 326 229 477 3.14e-26 108
MS.gene048067.t1 MTR_7g063880 31.164 292 160 9 20 303 209 467 6.19e-26 107
MS.gene048067.t1 MTR_7g063880 29.167 240 141 9 62 291 5 225 1.66e-15 77.4
MS.gene048067.t1 MTR_7g063670 30.796 289 158 7 20 304 207 457 4.36e-25 105
MS.gene048067.t1 MTR_7g063660 32.069 290 174 8 20 304 206 477 6.39e-25 105
MS.gene048067.t1 MTR_7g063660 30.672 238 137 9 63 291 4 222 2.16e-17 83.2
MS.gene048067.t1 MTR_7g063900 32.168 286 175 8 20 298 209 482 2.79e-24 103
MS.gene048067.t1 MTR_7g063900 30.705 241 133 11 63 291 7 225 7.65e-17 81.3
MS.gene048067.t1 MTR_4g019350 51.515 99 36 2 126 222 1 89 7.74e-24 94.0
MS.gene048067.t1 MTR_7g100420 32.624 282 163 7 19 294 133 393 3.62e-23 99.4
MS.gene048067.t1 MTR_7g066290 31.970 269 108 7 35 297 233 432 3.95e-23 99.4
MS.gene048067.t1 MTR_7g066290 26.577 222 135 9 68 277 15 220 5.09e-11 63.5
MS.gene048067.t1 MTR_3g065200 31.466 232 132 7 65 293 209 416 3.87e-22 97.1
MS.gene048067.t1 MTR_7g063820 31.399 293 174 7 20 304 211 484 5.23e-22 96.7
MS.gene048067.t1 MTR_7g063820 27.426 237 152 7 62 291 4 227 3.91e-13 70.1
MS.gene048067.t1 MTR_7g063830 30.035 283 141 7 20 298 209 438 5.68e-22 96.3
MS.gene048067.t1 MTR_7g063830 28.696 230 138 8 71 291 13 225 1.24e-12 68.6
MS.gene048067.t1 MTR_7g066490 28.826 281 126 7 20 298 199 407 1.81e-20 91.7
MS.gene048067.t1 MTR_8g445860 30.769 234 139 8 68 292 13 232 5.74e-20 87.8
MS.gene048067.t1 MTR_1g115440 30.000 240 148 7 62 292 5 233 1.36e-19 86.7
MS.gene048067.t1 MTR_7g063890 33.904 292 162 10 20 298 225 498 5.91e-19 87.8
MS.gene048067.t1 MTR_7g063890 30.870 230 132 9 71 291 30 241 3.23e-15 76.6
MS.gene048067.t1 MTR_0150s0010 30.252 238 148 8 62 292 6 232 1.16e-18 84.0
MS.gene048067.t1 MTR_7g063850 28.395 243 152 6 66 304 64 288 2.42e-18 84.3
MS.gene048067.t1 MTR_8g023240 30.962 239 132 9 68 296 272 487 4.56e-18 85.1
MS.gene048067.t1 MTR_7g058700 36.691 139 65 2 16 151 720 838 1.19e-17 84.3
MS.gene048067.t1 MTR_1g014060 33.333 204 104 5 98 298 210 384 1.54e-17 83.2
MS.gene048067.t1 MTR_2g029400 27.941 272 157 7 20 286 166 403 6.03e-16 78.6
MS.gene048067.t1 MTR_2g029400 36.036 111 62 3 56 159 4 112 8.29e-11 63.2
MS.gene048067.t1 MTR_1g115450 41.772 79 44 1 68 144 7 85 1.61e-14 70.1
MS.gene048067.t1 MTR_0150s0030 36.264 91 54 2 62 149 2 91 3.43e-13 68.2
MS.gene048067.t1 MTR_1g115455 27.615 239 151 7 62 292 3 227 4.66e-13 68.2
MS.gene048067.t1 MTR_8g445910 33.645 107 62 2 62 165 2 102 6.33e-13 68.2
MS.gene048067.t1 MTR_8g044880 37.778 90 52 2 62 148 2 90 1.08e-12 67.8
MS.gene048067.t1 MTR_8g445920 39.394 66 40 0 68 133 10 75 5.56e-12 62.0
MS.gene048067.t1 MTR_8g044890 39.394 66 40 0 68 133 10 75 5.56e-12 62.0
MS.gene048067.t1 MTR_7g066390 38.835 103 62 1 226 328 50 151 1.92e-11 62.0
MS.gene048067.t1 MTR_4g019330 42.222 90 49 2 215 303 97 184 2.36e-11 62.4
MS.gene048067.t1 MTR_7g066480 39.806 103 51 2 207 298 319 421 2.80e-11 64.3
MS.gene048067.t1 MTR_1g115470 40.506 79 45 1 68 144 6 84 7.39e-11 63.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene048067.t1 AT5G01140 30.097 309 185 8 20 326 192 471 1.18e-23 101
MS.gene048067.t1 AT5G01120 29.861 288 171 9 20 294 216 485 6.37e-23 99.4
MS.gene048067.t1 AT5G01120 27.468 233 149 9 69 291 10 232 2.53e-13 70.9
MS.gene048067.t1 AT3G09120 30.043 233 146 8 68 292 6 229 4.69e-22 95.1
MS.gene048067.t1 AT5G01130 28.881 277 155 9 20 294 204 440 3.18e-20 91.3
MS.gene048067.t1 AT5G01130 28.517 263 151 11 63 314 4 240 6.81e-15 75.5
MS.gene048067.t1 AT3G09140 29.577 284 170 6 20 294 255 517 2.63e-19 89.0
MS.gene048067.t1 AT3G09140 27.751 209 137 6 63 259 4 210 7.46e-15 75.5
MS.gene048067.t1 AT3G09140 29.225 284 171 6 20 294 227 489 5.76e-19 87.8
MS.gene048067.t1 AT3G09140 28.099 242 159 7 63 291 4 243 2.16e-17 83.2
MS.gene048067.t1 AT5G01150 28.821 229 127 6 77 294 272 475 3.53e-17 82.4
MS.gene048067.t1 AT5G01150 27.237 257 156 9 63 301 4 247 9.13e-13 69.3
MS.gene048067.t1 AT5G37320 28.139 231 128 7 77 299 280 480 4.60e-16 79.3
MS.gene048067.t1 AT5G43240 27.922 308 163 11 15 294 69 345 1.86e-15 76.6
MS.gene048067.t1 AT5G43240 29.583 240 120 8 77 294 277 489 3.30e-15 76.6
MS.gene048067.t1 AT5G43240 27.039 233 149 7 71 291 12 235 3.80e-12 67.4
MS.gene048067.t1 AT5G43240 29.583 240 120 8 77 294 277 489 3.30e-15 76.6
MS.gene048067.t1 AT5G43240 27.039 233 149 7 71 291 12 235 3.80e-12 67.4
MS.gene048067.t1 AT5G43240 29.583 240 120 8 77 294 277 489 3.30e-15 76.6
MS.gene048067.t1 AT5G43240 27.039 233 149 7 71 291 12 235 3.80e-12 67.4

Find 60 sgRNAs with CRISPR-Local

Find 81 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGGCGGGCTGTCTTTGTTCT+TGG 0.220660 4.4:-82147092 None:intergenic
AATCACTTTCTGCTAATTCT+TGG 0.239902 4.4:+82147689 MS.gene048067:CDS
CCCACCGCTATGTATTATTA+TGG 0.303524 4.4:+82147454 MS.gene048067:CDS
ATCAACTTGCTAGGAGATCT+TGG 0.315366 4.4:-82147601 None:intergenic
TTCCACTCGCAAGATTAAAA+TGG 0.319825 4.4:+82147147 MS.gene048067:CDS
ATAACTCAGTGTACAAGTTT+AGG 0.324914 4.4:-82148305 None:intergenic
AGGGTCCATTCTAAAGGTTT+TGG 0.333963 4.4:+82147270 MS.gene048067:CDS
CTCTGACTGCTGATGTAAAC+TGG 0.334051 4.4:-82147117 None:intergenic
AGCTTCCTTACAATACCATT+AGG 0.351633 4.4:+82147250 MS.gene048067:CDS
ACTAACAAGTTTACATTACT+TGG 0.363520 4.4:-82146965 None:intergenic
TCTTGGATCCATTGCAGTTA+AGG 0.368661 4.4:-82147584 None:intergenic
GCTTCCTTACAATACCATTA+GGG 0.381396 4.4:+82147251 MS.gene048067:CDS
GGAATGTTGAGGGGCTGCTT+TGG 0.386519 4.4:-82147418 None:intergenic
ACCATTAGGGTCCATTCTAA+AGG 0.425324 4.4:+82147264 MS.gene048067:CDS
AGGAGTGATTTCTAAGACAA+TGG 0.425580 4.4:+82147540 MS.gene048067:CDS
CAGCAGCGCATGAGAGGTTA+AGG 0.431836 4.4:-82147044 None:intergenic
ACTAGTTTGAACACACTTTG+AGG 0.444744 4.4:-82148258 None:intergenic
ACCATCCAAAACCTTTAGAA+TGG 0.447128 4.4:-82147275 None:intergenic
ACTGCTGATGTAAACTGGTT+TGG 0.459661 4.4:-82147112 None:intergenic
GAGAGGTTAAGGTGTACTTC+AGG 0.462590 4.4:-82147033 None:intergenic
CCTGGTGTCGCACCACAGTT+TGG 0.469731 4.4:+82147391 MS.gene048067:CDS
TTATAGTACAATAGAAATAC+CGG 0.479232 4.4:+82147516 MS.gene048067:CDS
ACCTTTAGAATGGACCCTAA+TGG 0.482523 4.4:-82147265 None:intergenic
TTTCACCTGAGGAATGTTGA+GGG 0.491518 4.4:-82147428 None:intergenic
TGCAATGTGTTACTCAATCC+TGG 0.497575 4.4:+82147373 MS.gene048067:CDS
AGACCAGCATTATATGTTGT+AGG 0.499652 4.4:+82147649 MS.gene048067:CDS
CAAAGTGTGTTCAAACTAGT+AGG 0.500953 4.4:+82148261 MS.gene048067:CDS
CCAAACTGTGGTGCGACACC+AGG 0.503303 4.4:-82147391 None:intergenic
GTACAAGAGTGTAGAGAACT+TGG 0.512946 4.4:+82147327 MS.gene048067:CDS
CTTTCACTTCAAGATCATCA+AGG 0.513607 4.4:-82147741 None:intergenic
GCTGATGTAAACTGGTTTGG+CGG 0.517872 4.4:-82147109 None:intergenic
TGGACCCTAATGGTATTGTA+AGG 0.519001 4.4:-82147255 None:intergenic
GGAGTGATTTCTAAGACAAT+GGG 0.529024 4.4:+82147541 MS.gene048067:CDS
ACATCTTTAATCAACTTGCT+AGG 0.531332 4.4:-82147610 None:intergenic
TGCAAAAGCCTTAACTGCAA+TGG 0.542350 4.4:+82147576 MS.gene048067:CDS
GTCTTAGAAATCACTCCTCC+CGG 0.545356 4.4:-82147535 None:intergenic
CAAGTTGATTAAAGATGTGG+TGG 0.546492 4.4:+82147615 MS.gene048067:CDS
TTTCACTTCAAGATCATCAA+GGG 0.554864 4.4:-82147740 None:intergenic
TCTCCTACAACATATAATGC+TGG 0.557766 4.4:-82147652 None:intergenic
TAGAGAACTTGGACTCTTCG+TGG 0.558135 4.4:+82147338 MS.gene048067:CDS
GCAGCCCCTCAACATTCCTC+AGG 0.561565 4.4:+82147423 MS.gene048067:CDS
CTGATGTAAACTGGTTTGGC+GGG 0.571436 4.4:-82147108 None:intergenic
TTGATGTCAAAGAAGCTGCT+AGG 0.575782 4.4:-82148172 None:intergenic
TAGCAAGTTGATTAAAGATG+TGG 0.577463 4.4:+82147612 MS.gene048067:CDS
TCAAAATCTACACTCACTAG+TGG 0.579892 4.4:+82148191 MS.gene048067:CDS
ACCATAATAATACATAGCGG+TGG 0.605154 4.4:-82147455 None:intergenic
ATTTGTCAGCAGCGCATGAG+AGG 0.606113 4.4:-82147050 None:intergenic
TTCACCTGAGGAATGTTGAG+GGG 0.607189 4.4:-82147427 None:intergenic
GATAAGCATTGGTGAAGCTG+AGG 0.615886 4.4:+82147765 MS.gene048067:CDS
CCATAATAATACATAGCGGT+GGG 0.618665 4.4:-82147454 None:intergenic
TGGTACAAGAAAGCAAGAGA+AGG 0.627443 4.4:+82147474 MS.gene048067:CDS
TTCACTTCAAGATCATCAAG+GGG 0.628276 4.4:-82147739 None:intergenic
AGGTTAAGGTGTACTTCAGG+AGG 0.632211 4.4:-82147030 None:intergenic
GAAGTGAAAGTGATAAGCAT+TGG 0.646678 4.4:+82147754 MS.gene048067:CDS
AAGTTGATTAAAGATGTGGT+GGG 0.652161 4.4:+82147616 MS.gene048067:CDS
ATGTCAAAGAAGCTGCTAGG+AGG 0.665227 4.4:-82148169 None:intergenic
TGCTTTGGACAACCAAACTG+TGG 0.670639 4.4:-82147403 None:intergenic
TATAGTACAATAGAAATACC+GGG 0.703320 4.4:+82147517 MS.gene048067:CDS
TGTACCATAATAATACATAG+CGG 0.764106 4.4:-82147458 None:intergenic
AGTACAATAGAAATACCGGG+AGG 0.798359 4.4:+82147520 MS.gene048067:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTATAGTACAATAGAAATAC+CGG + chr4.4:82147516-82147535 MS.gene048067:CDS 20.0%
!!! AAAAGAGTACTTAATCATTT+TGG - chr4.4:82148089-82148108 None:intergenic 20.0%
!!! TTTTTAGTAAACAATCATGT+TGG + chr4.4:82147827-82147846 MS.gene048067:intron 20.0%
! ACTAACAAGTTTACATTACT+TGG - chr4.4:82146968-82146987 None:intergenic 25.0%
! TATAGTACAATAGAAATACC+GGG + chr4.4:82147517-82147536 MS.gene048067:CDS 25.0%
! TGTACCATAATAATACATAG+CGG - chr4.4:82147461-82147480 None:intergenic 25.0%
!! GAAGTCAACAAATTTTTCAT+TGG - chr4.4:82147224-82147243 None:intergenic 25.0%
AATCACTTTCTGCTAATTCT+TGG + chr4.4:82147689-82147708 MS.gene048067:CDS 30.0%
ACTAACATAAGACACATTCA+GGG - chr4.4:82147912-82147931 None:intergenic 30.0%
TACTAACATAAGACACATTC+AGG - chr4.4:82147913-82147932 None:intergenic 30.0%
TTGTTGATACTCTTCCTTTA+TGG + chr4.4:82148124-82148143 MS.gene048067:intron 30.0%
TTTCACTTCAAGATCATCAA+GGG - chr4.4:82147743-82147762 None:intergenic 30.0%
! AAGTTGATTAAAGATGTGGT+GGG + chr4.4:82147616-82147635 MS.gene048067:CDS 30.0%
! ACATCTTTAATCAACTTGCT+AGG - chr4.4:82147613-82147632 None:intergenic 30.0%
!! TAGCAAGTTGATTAAAGATG+TGG + chr4.4:82147612-82147631 MS.gene048067:CDS 30.0%
ACCATAATAATACATAGCGG+TGG - chr4.4:82147458-82147477 None:intergenic 35.0%
AGACCAGCATTATATGTTGT+AGG + chr4.4:82147649-82147668 MS.gene048067:CDS 35.0%
AGCTTCCTTACAATACCATT+AGG + chr4.4:82147250-82147269 MS.gene048067:CDS 35.0%
CAAAGTGTGTTCAAACTAGT+AGG + chr4.4:82148261-82148280 MS.gene048067:CDS 35.0%
CCATAATAATACATAGCGGT+GGG - chr4.4:82147457-82147476 None:intergenic 35.0%
CTTTCACTTCAAGATCATCA+AGG - chr4.4:82147744-82147763 None:intergenic 35.0%
GAAGTGAAAGTGATAAGCAT+TGG + chr4.4:82147754-82147773 MS.gene048067:CDS 35.0%
GCTTCCTTACAATACCATTA+GGG + chr4.4:82147251-82147270 MS.gene048067:CDS 35.0%
TCAAAATCTACACTCACTAG+TGG + chr4.4:82148191-82148210 MS.gene048067:CDS 35.0%
TCTCCTACAACATATAATGC+TGG - chr4.4:82147655-82147674 None:intergenic 35.0%
TGGTCTTCTGTTAATCAGTT+TGG - chr4.4:82147946-82147965 None:intergenic 35.0%
TTCACTTCAAGATCATCAAG+GGG - chr4.4:82147742-82147761 None:intergenic 35.0%
TTCCACTCGCAAGATTAAAA+TGG + chr4.4:82147147-82147166 MS.gene048067:CDS 35.0%
! ACCATCCAAAACCTTTAGAA+TGG - chr4.4:82147278-82147297 None:intergenic 35.0%
! ACTAGTTTGAACACACTTTG+AGG - chr4.4:82148261-82148280 None:intergenic 35.0%
! TTGGATGGTAACTCTTTTGT+TGG + chr4.4:82147289-82147308 MS.gene048067:CDS 35.0%
!! AGGAGTGATTTCTAAGACAA+TGG + chr4.4:82147540-82147559 MS.gene048067:CDS 35.0%
!! ATCCATTTTAATCTTGCGAG+TGG - chr4.4:82147152-82147171 None:intergenic 35.0%
!! CAAGTTGATTAAAGATGTGG+TGG + chr4.4:82147615-82147634 MS.gene048067:CDS 35.0%
!! GGAGTGATTTCTAAGACAAT+GGG + chr4.4:82147541-82147560 MS.gene048067:CDS 35.0%
!!! TCCATTCTAAAGGTTTTGGA+TGG + chr4.4:82147274-82147293 MS.gene048067:CDS 35.0%
ACCATTAGGGTCCATTCTAA+AGG + chr4.4:82147264-82147283 MS.gene048067:CDS 40.0%
ACTGCTGATGTAAACTGGTT+TGG - chr4.4:82147115-82147134 None:intergenic 40.0%
AGTACAATAGAAATACCGGG+AGG + chr4.4:82147520-82147539 MS.gene048067:CDS 40.0%
CCCACCGCTATGTATTATTA+TGG + chr4.4:82147454-82147473 MS.gene048067:CDS 40.0%
CTGCAAAGTGCAAACCATAA+AGG - chr4.4:82148141-82148160 None:intergenic 40.0%
GTGACACCATCTCACATTTA+AGG - chr4.4:82147854-82147873 None:intergenic 40.0%
GTTGGTCCTTAAATGTGAGA+TGG + chr4.4:82147845-82147864 MS.gene048067:intron 40.0%
TCTTGGATCCATTGCAGTTA+AGG - chr4.4:82147587-82147606 None:intergenic 40.0%
TGCAAAAGCCTTAACTGCAA+TGG + chr4.4:82147576-82147595 MS.gene048067:CDS 40.0%
TGGACCCTAATGGTATTGTA+AGG - chr4.4:82147258-82147277 None:intergenic 40.0%
TGGTACAAGAAAGCAAGAGA+AGG + chr4.4:82147474-82147493 MS.gene048067:CDS 40.0%
TTGATGTCAAAGAAGCTGCT+AGG - chr4.4:82148175-82148194 None:intergenic 40.0%
TTTCACCTGAGGAATGTTGA+GGG - chr4.4:82147431-82147450 None:intergenic 40.0%
! CTTTATGGTTTGCACTTTGC+AGG + chr4.4:82148139-82148158 MS.gene048067:intron 40.0%
! GTACAAGAGTGTAGAGAACT+TGG + chr4.4:82147327-82147346 MS.gene048067:CDS 40.0%
! TGACAGTACTCGCACTTTTA+AGG + chr4.4:82148062-82148081 MS.gene048067:intron 40.0%
! TGCAATGTGTTACTCAATCC+TGG + chr4.4:82147373-82147392 MS.gene048067:CDS 40.0%
! TTTTCACCTGAGGAATGTTG+AGG - chr4.4:82147432-82147451 None:intergenic 40.0%
!! ACCTTTAGAATGGACCCTAA+TGG - chr4.4:82147268-82147287 None:intergenic 40.0%
!! AGGGTCCATTCTAAAGGTTT+TGG + chr4.4:82147270-82147289 MS.gene048067:CDS 40.0%
!! ATCAACTTGCTAGGAGATCT+TGG - chr4.4:82147604-82147623 None:intergenic 40.0%
AAGACCAAACGAACCAACGA+AGG + chr4.4:82147959-82147978 MS.gene048067:intron 45.0%
ACCAACGAAGGACACATACA+AGG + chr4.4:82147971-82147990 MS.gene048067:intron 45.0%
AGGTTAAGGTGTACTTCAGG+AGG - chr4.4:82147033-82147052 None:intergenic 45.0%
ATGTCAAAGAAGCTGCTAGG+AGG - chr4.4:82148172-82148191 None:intergenic 45.0%
CTCTGACTGCTGATGTAAAC+TGG - chr4.4:82147120-82147139 None:intergenic 45.0%
GAGAGGTTAAGGTGTACTTC+AGG - chr4.4:82147036-82147055 None:intergenic 45.0%
GTCTTAGAAATCACTCCTCC+CGG - chr4.4:82147538-82147557 None:intergenic 45.0%
TAGAGAACTTGGACTCTTCG+TGG + chr4.4:82147338-82147357 MS.gene048067:CDS 45.0%
TCCTTGTATGTGTCCTTCGT+TGG - chr4.4:82147975-82147994 None:intergenic 45.0%
TTCACCTGAGGAATGTTGAG+GGG - chr4.4:82147430-82147449 None:intergenic 45.0%
TTGGTGAAGCTGAGGTAAAG+TGG + chr4.4:82147773-82147792 MS.gene048067:intron 45.0%
! TGCTTTGGACAACCAAACTG+TGG - chr4.4:82147406-82147425 None:intergenic 45.0%
!! CTGATGTAAACTGGTTTGGC+GGG - chr4.4:82147111-82147130 None:intergenic 45.0%
!! GATAAGCATTGGTGAAGCTG+AGG + chr4.4:82147765-82147784 MS.gene048067:CDS 45.0%
!! GCTGATGTAAACTGGTTTGG+CGG - chr4.4:82147112-82147131 None:intergenic 45.0%
ATTTGTCAGCAGCGCATGAG+AGG - chr4.4:82147053-82147072 None:intergenic 50.0%
!! GTGTCCTTCGTTGGTTCGTT+TGG - chr4.4:82147966-82147985 None:intergenic 50.0%
CAGCAGCGCATGAGAGGTTA+AGG - chr4.4:82147047-82147066 None:intergenic 55.0%
GGAATGTTGAGGGGCTGCTT+TGG - chr4.4:82147421-82147440 None:intergenic 55.0%
!! TGGCGGGCTGTCTTTGTTCT+TGG - chr4.4:82147095-82147114 None:intergenic 55.0%
CCAAACTGTGGTGCGACACC+AGG - chr4.4:82147394-82147413 None:intergenic 60.0%
GCAGCCCCTCAACATTCCTC+AGG + chr4.4:82147423-82147442 MS.gene048067:CDS 60.0%
! GCGGTGGGTGTTTTCACCTG+AGG - chr4.4:82147442-82147461 None:intergenic 60.0%
!! CCTGGTGTCGCACCACAGTT+TGG + chr4.4:82147391-82147410 MS.gene048067:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.4 gene 82146962 82148322 82146962 ID=MS.gene048067
chr4.4 mRNA 82146962 82148322 82146962 ID=MS.gene048067.t1;Parent=MS.gene048067
chr4.4 exon 82146962 82147786 82146962 ID=MS.gene048067.t1.exon1;Parent=MS.gene048067.t1
chr4.4 CDS 82146962 82147786 82146962 ID=cds.MS.gene048067.t1;Parent=MS.gene048067.t1
chr4.4 exon 82148161 82148322 82148161 ID=MS.gene048067.t1.exon2;Parent=MS.gene048067.t1
chr4.4 CDS 82148161 82148322 82148161 ID=cds.MS.gene048067.t1;Parent=MS.gene048067.t1
Gene Sequence

>MS.gene048067

ATGCCAAGTAATGTAAACTTGTTAGTGAAATATTCTGACAAACACAATTTTGATGTACAGATGTTGAACCTCCTGAAGTACACCTTAACCTCTCATGCGCTGCTGACAAATACAATTTTGAAAAGCTGCTCCAAGAACAAAGACAGCCCGCCAAACCAGTTTACATCAGCAGTCAGAGTAAAAACTTCCACTCGCAAGATTAAAATGGATGTTAAGCTAGTGCGAAGTAAATCTCAGAAAAAGATAATCTTTGCAGAAGCCAATGAAAAATTTGTTGACTTCATGTTTAGCTTCCTTACAATACCATTAGGGTCCATTCTAAAGGTTTTGGATGGTAACTCTTTTGTTGGATGTGTTGATAATTTGTACAAGAGTGTAGAGAACTTGGACTCTTCGTGGTGTACAGACTCATGCAATGTGTTACTCAATCCTGGTGTCGCACCACAGTTTGGTTGTCCAAAGCAGCCCCTCAACATTCCTCAGGTGAAAACACCCACCGCTATGTATTATTATGGTACAAGAAAGCAAGAGAAGGATTATCGCTATACTGATTGTTATAGTACAATAGAAATACCGGGAGGAGTGATTTCTAAGACAATGGGATCAATTTATGATGCAAAAGCCTTAACTGCAATGGATCCAAGATCTCCTAGCAAGTTGATTAAAGATGTGGTGGGATTTGTAAAGAGACCAGCATTATATGTTGTAGGAGATGATCTAAAAGTAAAATCACTTTCTGCTAATTCTTGGCTTTCATATTTGAAAGAATTGAGCTTTCCCCTTGATGATCTTGAAGTGAAAGTGATAAGCATTGGTGAAGCTGAGGTAAAGTGGCTATTACTACTGAATTTAAATATTTTTTTTCTTTTTAGTAAACAATCATGTTGGTCCTTAAATGTGAGATGGTGTCACTATAGTCTGAATGTAATAATATATCATAAAAACATCCCTGAATGTGTCTTATGTTAGTAATTTTATCAACCAAACTGATTAACAGAAGACCAAACGAACCAACGAAGGACACATACAAGGATTAAACTGAATAAGAAAACACAAATTCTTGTATGTTTTTCTAGTTTTGAAAGATTCGTGAATTATAATGACAGTACTCGCACTTTTAAGGACCAAAATGATTAAGTACTCTTTTGTTTGTTTTTCTTTGTTGTTGATACTCTTCCTTTATGGTTTGCACTTTGCAGGCTCTGAGCCTCCTAGCAGCTTCTTTGACATCAAAATCTACACTCACTAGTGGACTAGAAGAATTTTTTAATATGCAAAAACAAGATTCAAATTTGACCTCAAAGTGTGTTCAAACTAGTAGGCTTGATAAAGTTGCAAAAAAGCCTAAACTTGTACACTGA
Protein sequence

>MS.gene048067.t1

MPSNVNLLVKYSDKHNFDVQMLNLLKYTLTSHALLTNTILKSCSKNKDSPPNQFTSAVRVKTSTRKIKMDVKLVRSKSQKKIIFAEANEKFVDFMFSFLTIPLGSILKVLDGNSFVGCVDNLYKSVENLDSSWCTDSCNVLLNPGVAPQFGCPKQPLNIPQVKTPTAMYYYGTRKQEKDYRYTDCYSTIEIPGGVISKTMGSIYDAKALTAMDPRSPSKLIKDVVGFVKRPALYVVGDDLKVKSLSANSWLSYLKELSFPLDDLEVKVISIGEAEALSLLAASLTSKSTLTSGLEEFFNMQKQDSNLTSKCVQTSRLDKVAKKPKLVH