AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50804

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50804.t1 MTR_4g017470 77.030 431 60 2 1 430 101 493 0.0 655
MS.gene50804.t1 MTR_4g017470 26.482 253 111 5 169 403 9 204 1.22e-11 66.6
MS.gene50804.t1 MTR_4g017410 74.884 430 80 3 1 426 98 503 0.0 635
MS.gene50804.t1 MTR_4g017410 28.458 253 140 10 171 401 8 241 1.38e-13 72.8
MS.gene50804.t1 MTR_4g017550 73.153 406 95 5 1 404 77 470 0.0 570
MS.gene50804.t1 MTR_4g017830 67.458 421 125 5 1 416 97 510 0.0 561
MS.gene50804.t1 MTR_4g017440 68.599 414 119 4 1 413 105 508 0.0 561
MS.gene50804.t1 MTR_4g019340 67.662 402 99 4 1 397 128 503 0.0 541
MS.gene50804.t1 MTR_4g017610 58.561 403 140 6 1 403 100 475 1.03e-155 449
MS.gene50804.t1 MTR_4g019310 40.793 429 216 14 1 415 95 499 9.07e-83 263
MS.gene50804.t1 MTR_4g019310 26.939 245 149 6 172 400 8 238 8.90e-13 70.5
MS.gene50804.t1 MTR_7g058710 33.718 433 239 14 1 413 79 483 9.90e-56 192
MS.gene50804.t1 MTR_7g058710 26.400 250 141 8 163 400 3 221 8.53e-15 76.6
MS.gene50804.t1 MTR_7g066340 31.354 421 217 11 1 407 91 453 5.91e-53 184
MS.gene50804.t1 MTR_7g066340 25.600 250 149 8 165 400 5 231 5.11e-14 73.9
MS.gene50804.t1 MTR_7g066570 32.584 445 247 13 1 426 93 503 1.73e-52 185
MS.gene50804.t1 MTR_7g066350 32.075 424 217 10 1 412 88 452 1.74e-50 177
MS.gene50804.t1 MTR_7g066410 33.853 449 232 14 1 408 89 513 2.72e-50 178
MS.gene50804.t1 MTR_7g066430 34.332 434 244 12 1 416 83 493 9.71e-50 176
MS.gene50804.t1 MTR_7g066260 31.442 423 214 13 1 404 94 459 5.50e-47 169
MS.gene50804.t1 MTR_7g066260 29.796 245 131 9 172 400 15 234 2.22e-14 75.5
MS.gene50804.t1 MTR_7g066400 32.706 425 214 15 1 412 75 440 5.16e-46 165
MS.gene50804.t1 MTR_7g062280 31.222 442 236 14 1 413 89 491 4.13e-41 152
MS.gene50804.t1 MTR_7g062280 26.316 247 145 9 173 400 11 239 5.49e-11 64.7
MS.gene50804.t1 MTR_1g041315 30.806 422 198 16 1 391 93 451 1.15e-40 151
MS.gene50804.t1 MTR_7g063660 30.607 428 244 13 1 413 88 477 1.08e-39 148
MS.gene50804.t1 MTR_7g063660 36.000 125 69 3 171 288 8 128 1.41e-14 75.9
MS.gene50804.t1 MTR_7g063900 31.905 420 249 14 1 408 89 483 1.57e-39 148
MS.gene50804.t1 MTR_7g063900 27.049 244 137 7 171 400 9 225 1.36e-13 72.8
MS.gene50804.t1 MTR_7g012680 31.395 430 207 14 1 413 93 451 9.26e-39 145
MS.gene50804.t1 MTR_7g063880 29.812 426 239 15 1 413 90 468 1.47e-37 142
MS.gene50804.t1 MTR_7g066280 26.905 420 256 12 1 403 1 386 2.70e-35 135
MS.gene50804.t1 MTR_7g062300 28.947 418 249 11 1 406 91 472 3.41e-35 136
MS.gene50804.t1 MTR_7g062300 26.908 249 149 8 163 400 3 229 3.07e-12 68.6
MS.gene50804.t1 MTR_7g063840 29.953 424 247 13 1 413 89 473 1.86e-34 134
MS.gene50804.t1 MTR_7g063890 30.575 435 244 13 1 413 106 504 2.04e-34 134
MS.gene50804.t1 MTR_7g066360 31.971 416 209 16 1 404 93 446 4.59e-34 133
MS.gene50804.t1 MTR_7g066580 29.147 422 230 13 1 407 74 441 7.78e-34 132
MS.gene50804.t1 MTR_7g063820 29.673 428 255 10 1 413 88 484 1.94e-33 131
MS.gene50804.t1 MTR_7g063830 28.873 426 220 12 1 413 89 444 4.25e-32 127
MS.gene50804.t1 MTR_7g066310 26.988 415 170 11 1 403 86 379 2.63e-30 121
MS.gene50804.t1 MTR_7g066310 33.945 109 65 2 167 268 2 110 6.51e-11 64.3
MS.gene50804.t1 MTR_7g058700 32.982 285 131 9 1 255 584 838 3.81e-30 124
MS.gene50804.t1 MTR_3g007740 26.988 415 247 11 2 408 77 443 4.37e-27 112
MS.gene50804.t1 MTR_7g066290 33.569 283 153 9 1 263 94 361 2.59e-26 110
MS.gene50804.t1 MTR_7g063920 27.208 419 227 12 1 413 90 436 9.98e-26 108
MS.gene50804.t1 MTR_7g063670 29.009 424 240 15 2 413 83 457 1.47e-25 108
MS.gene50804.t1 MTR_7g066680 30.037 273 159 7 123 386 37 286 6.01e-23 99.8
MS.gene50804.t1 MTR_4g019350 51.020 98 32 2 230 326 1 83 1.85e-22 91.7
MS.gene50804.t1 MTR_7g066480 32.601 273 143 10 1 254 95 345 4.94e-22 98.2
MS.gene50804.t1 MTR_2g029400 25.743 404 198 11 1 395 93 403 4.76e-21 95.1
MS.gene50804.t1 MTR_8g445860 31.535 241 122 8 177 401 19 232 8.49e-20 88.6
MS.gene50804.t1 MTR_1g014060 34.259 216 103 7 202 413 210 390 1.79e-15 78.2
MS.gene50804.t1 MTR_7g066490 28.626 262 154 7 1 254 95 331 2.78e-15 77.8
MS.gene50804.t1 MTR_1g115440 28.916 249 150 8 163 401 2 233 3.24e-15 75.5
MS.gene50804.t1 MTR_7g100420 28.082 292 167 7 123 403 134 393 2.28e-14 74.7
MS.gene50804.t1 MTR_1g115450 50.000 74 34 2 177 248 13 85 8.20e-14 68.9
MS.gene50804.t1 MTR_8g023240 24.885 217 141 5 59 260 159 368 2.10e-13 72.4
MS.gene50804.t1 MTR_4g019240 63.462 52 19 0 365 416 112 163 1.07e-12 66.6
MS.gene50804.t1 MTR_0150s0030 40.741 81 45 2 172 249 8 88 1.40e-11 63.9
MS.gene50804.t1 MTR_0150s0010 39.535 86 50 1 165 248 3 88 1.83e-11 64.3
MS.gene50804.t1 MTR_1g115455 40.506 79 45 1 172 248 9 87 1.94e-11 64.3
MS.gene50804.t1 MTR_8g445910 41.975 81 44 2 172 249 8 88 3.82e-11 63.9
MS.gene50804.t1 MTR_8g044880 42.500 80 43 2 172 248 8 87 4.81e-11 63.5
MS.gene50804.t1 MTR_8g445920 36.709 79 50 0 165 243 3 81 9.36e-11 59.3
MS.gene50804.t1 MTR_8g044890 36.709 79 50 0 165 243 3 81 9.36e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50804.t1 AT5G01130 29.157 415 241 11 1 413 88 451 4.31e-42 154
MS.gene50804.t1 AT5G01120 29.147 422 256 14 1 403 88 485 8.04e-41 152
MS.gene50804.t1 AT5G43240 29.812 426 252 12 1 403 88 489 9.15e-41 152
MS.gene50804.t1 AT5G43240 29.812 426 252 12 1 403 88 489 9.15e-41 152
MS.gene50804.t1 AT5G43240 29.812 426 252 12 1 403 88 489 9.15e-41 152
MS.gene50804.t1 AT5G01140 28.910 422 256 13 1 413 72 458 8.22e-39 146
MS.gene50804.t1 AT3G09140 29.025 441 260 15 1 415 88 501 1.17e-34 135
MS.gene50804.t1 AT3G09140 27.615 239 161 7 171 400 8 243 6.41e-15 77.0
MS.gene50804.t1 AT5G01150 27.027 444 258 13 1 418 88 491 5.46e-34 133
MS.gene50804.t1 AT5G43240 29.345 351 214 7 65 403 17 345 7.24e-33 127
MS.gene50804.t1 AT3G09140 27.292 469 260 16 1 415 88 529 6.04e-30 122
MS.gene50804.t1 AT3G09140 25.941 239 157 8 171 396 8 239 8.37e-12 67.4
MS.gene50804.t1 AT5G37320 27.040 429 255 14 1 408 89 480 1.13e-29 120
MS.gene50804.t1 AT3G09110 32.917 240 143 7 2 233 89 318 2.96e-27 111
MS.gene50804.t1 AT3G09120 31.933 238 142 8 1 226 84 313 6.69e-24 101
MS.gene50804.t1 AT3G09120 25.833 240 154 7 171 401 5 229 3.07e-14 73.6

Find 93 sgRNAs with CRISPR-Local

Find 110 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ATAGGTGGTGGCTGCATTTC+AGG 0.201101 4.1:+80176362 None:intergenic
TAATAGACAGCCTAAGAATT+TGG 0.219049 4.1:-80177136 MS.gene50804:CDS
AGGTGGGCTGTCTTTGTTCT+TGG 0.232574 4.1:+80176712 None:intergenic
CCACCTATTACATATTATTA+TGG 0.255078 4.1:-80176347 MS.gene50804:CDS
AGAAAATGGATCGTTGTTTA+TGG 0.263995 4.1:-80177067 MS.gene50804:CDS
CAACGGATCACAAGTTGATA+TGG 0.270644 4.1:-80176171 MS.gene50804:CDS
AAAAGAGTTGTTTCTTGTTT+AGG 0.272026 4.1:+80175844 None:intergenic
CATGCAGCAATTGATGGAAT+TGG 0.295151 4.1:-80177001 MS.gene50804:CDS
CACCGGTGACATCAGGAAAA+TGG 0.299634 4.1:-80176654 MS.gene50804:CDS
CCATCAGAATCCAAATTCTT+AGG 0.304525 4.1:+80177126 None:intergenic
TAAACAAGAAACAACTCTTT+TGG 0.309535 4.1:-80175842 MS.gene50804:CDS
AACAAGAGAAGTAAAGATAG+AGG 0.310667 4.1:-80176267 MS.gene50804:CDS
AGCTTCCTTACGTTGCCATT+AGG 0.316633 4.1:-80176551 MS.gene50804:CDS
CTCTAACTGCTGATGCAAAT+TGG 0.325970 4.1:+80176687 None:intergenic
GTAAAGAGAGCAGCATTATA+TGG 0.327171 4.1:-80176143 MS.gene50804:CDS
GTACCATAATAATATGTAAT+AGG 0.339119 4.1:+80176344 None:intergenic
GCTTCCTTACGTTGCCATTA+GGG 0.349941 4.1:-80176550 MS.gene50804:CDS
TTAATTCTTTCAAATATAGA+AGG 0.350593 4.1:+80176075 None:intergenic
ATGCAGCAATTGATGGAATT+GGG 0.352067 4.1:-80177000 MS.gene50804:CDS
GGCGTCTTTATGACAGAAAA+TGG 0.355556 4.1:-80177081 MS.gene50804:CDS
TGTGATCCGTTGAGGGATCT+TGG 0.355842 4.1:+80176182 None:intergenic
AAGCGATTCACATGGATTCC+TGG 0.362784 4.1:+80177278 None:intergenic
GTGTAGGCTTTGAGCTTCCT+TGG 0.380217 4.1:-80175925 MS.gene50804:intron
GACTTCCATGCAGCAATTGA+TGG 0.383445 4.1:-80177007 MS.gene50804:CDS
CACACACAACATTGGCAAAC+AGG 0.390115 4.1:-80176947 MS.gene50804:CDS
CACAGGTACAGTGAAGGTTT+CGG 0.392605 4.1:+80177169 None:intergenic
GAGAGGCTCATGGGAGGTTA+AGG 0.396809 4.1:+80176760 None:intergenic
GCTACTTCTCAAAATTGTAT+TGG 0.405537 4.1:+80176736 None:intergenic
CTTCAAGATCGTCAAGAGAA+AGG 0.410498 4.1:+80176051 None:intergenic
TGCAAAATTCTTAACTGCAC+TGG 0.435564 4.1:-80176207 MS.gene50804:CDS
CTATATATAAACTCTTGCTT+TGG 0.439118 4.1:+80176314 None:intergenic
ATTCTTAGGCTGTCTATTAG+TGG 0.442822 4.1:+80177140 None:intergenic
AGATATACAACTGACCCTAA+TGG 0.442910 4.1:+80176536 None:intergenic
GGGAGGTTAAGGAATAATTC+AGG 0.442965 4.1:+80176771 None:intergenic
GTGATCCGTTGAGGGATCTT+GGG 0.444569 4.1:+80176183 None:intergenic
TTCTTAGGCTGTCTATTAGT+GGG 0.450651 4.1:+80177141 None:intergenic
TTTCAGGAATGTTGAGGGGC+TGG 0.452714 4.1:+80176378 None:intergenic
CTGCATTTCAGGAATGTTGA+GGG 0.452764 4.1:+80176373 None:intergenic
CTTGTTTAGGCACACTCAAA+AGG 0.455083 4.1:+80175857 None:intergenic
CGTCTTGCACCGGTGACATC+AGG 0.455219 4.1:-80176661 MS.gene50804:CDS
TGGAGAACTTGGACTCTTCA+TGG 0.456560 4.1:-80176463 MS.gene50804:CDS
CGCACTGTGTTACTCAATCC+TGG 0.466642 4.1:-80176428 MS.gene50804:CDS
CCTGGTGTAGCACACCAGTT+TGG 0.470247 4.1:-80176410 MS.gene50804:CDS
GTACAAGAGTGTGGAGAACT+TGG 0.471755 4.1:-80176474 MS.gene50804:CDS
GCTGCATTTCAGGAATGTTG+AGG 0.478547 4.1:+80176372 None:intergenic
ATTACATATTATTATGGTAC+AGG 0.484988 4.1:-80176341 MS.gene50804:CDS
AGCGATTCACATGGATTCCT+GGG 0.485112 4.1:+80177279 None:intergenic
AGAGCAGCATTATATGGTAT+AGG 0.490389 4.1:-80176137 MS.gene50804:CDS
GCTGGTTTCGACAACCAAAC+TGG 0.492728 4.1:+80176396 None:intergenic
GAAATCAACAAAATCTCCAT+TGG 0.495540 4.1:+80176580 None:intergenic
AGGAAAGAATTAGCAGAGAG+TGG 0.503286 4.1:+80176095 None:intergenic
ACTGCTGATGCAAATTGGTT+AGG 0.504158 4.1:+80176692 None:intergenic
CTCAAAATTGTATTGGTGAG+AGG 0.509830 4.1:+80176743 None:intergenic
AACGGATCACAAGTTGATAT+GGG 0.520381 4.1:-80176170 MS.gene50804:CDS
TCTTGACGATCTTGAAGCAA+AGG 0.520499 4.1:-80176045 MS.gene50804:CDS
TTCAGACTTGACTCAAATAG+AGG 0.521757 4.1:-80176974 MS.gene50804:CDS
ATGGAAGTCACAGAGGAACT+TGG 0.523683 4.1:+80177021 None:intergenic
ATCAACTTGTGATCCGTTGA+GGG 0.526068 4.1:+80176175 None:intergenic
ATTTGGATTCTGATGGTCAA+GGG 0.529572 4.1:-80177119 MS.gene50804:CDS
CCTAAGAATTTGGATTCTGA+TGG 0.538168 4.1:-80177126 MS.gene50804:CDS
TTGTATTGGTGAGAGGCTCA+TGG 0.539351 4.1:+80176750 None:intergenic
TCAAGATTTACCTTGACTAG+TGG 0.544138 4.1:-80175889 MS.gene50804:CDS
TAATAATATGTAATAGGTGG+TGG 0.544263 4.1:+80176350 None:intergenic
ACAAGAGAAGTAAAGATAGA+GGG 0.556606 4.1:-80176266 MS.gene50804:CDS
GACAAATGTTGCTTCATCCC+AGG 0.556708 4.1:-80177296 None:intergenic
CCAAACTGGTGTGCTACACC+AGG 0.558658 4.1:+80176410 None:intergenic
ATAATCTTTGCAGAAACCAA+TGG 0.568343 4.1:-80176596 MS.gene50804:CDS
CTGATGCAAATTGGTTAGGT+GGG 0.568436 4.1:+80176696 None:intergenic
TGTATTGGTGAGAGGCTCAT+GGG 0.577296 4.1:+80176751 None:intergenic
GTCTTTAAGTCCACTAGTCA+AGG 0.577600 4.1:+80175879 None:intergenic
GCTGATGCAAATTGGTTAGG+TGG 0.578014 4.1:+80176695 None:intergenic
CGAAACCTTCACTGTACCTG+TGG 0.582669 4.1:-80177168 MS.gene50804:CDS
AGAGAAGTAAAGATAGAGGG+AGG 0.592777 4.1:-80176263 MS.gene50804:CDS
CAAGGGAATAATGCACAAGA+TGG 0.595731 4.1:-80177102 MS.gene50804:CDS
TATCAACTTGTGATCCGTTG+AGG 0.597413 4.1:+80176174 None:intergenic
TGCAGCAATTGATGGAATTG+GGG 0.605020 4.1:-80176999 MS.gene50804:CDS
GAAGCAAAGGTGATGAGCAT+CGG 0.608043 4.1:-80176032 MS.gene50804:CDS
GTTAGAGCAACGTCTTGCAC+CGG 0.611909 4.1:-80176671 MS.gene50804:CDS
TGCATTTCAGGAATGTTGAG+GGG 0.616015 4.1:+80176374 None:intergenic
CAATTCCATCAATTGCTGCA+TGG 0.618654 4.1:+80177002 None:intergenic
CCATAATAATATGTAATAGG+TGG 0.620194 4.1:+80176347 None:intergenic
GAGGAAGTCACACACAACAT+TGG 0.621785 4.1:-80176955 MS.gene50804:CDS
AATTTGGATTCTGATGGTCA+AGG 0.623814 4.1:-80177120 MS.gene50804:CDS
CTGACCCTAATGGCAACGTA+AGG 0.626966 4.1:+80176546 None:intergenic
GATGAGCATCGGTGAAGCTG+AGG 0.627852 4.1:-80176021 MS.gene50804:intron
TTGATGTCAAGAATGCTCCA+AGG 0.669048 4.1:+80175908 None:intergenic
ACACAACATTGGCAAACAGG+AGG 0.681241 4.1:-80176944 MS.gene50804:intron
TTCATGCAAAGCGATTCACA+TGG 0.692991 4.1:+80177270 None:intergenic
TGGTAATTTGTACAAGAGTG+TGG 0.696549 4.1:-80176483 MS.gene50804:CDS
ATTGGTGAGAGGCTCATGGG+AGG 0.700980 4.1:+80176754 None:intergenic
GGAGTGATTTCTAAGTCACA+CGG 0.701071 4.1:-80176242 MS.gene50804:CDS
TTGCTGCATGGAAGTCACAG+AGG 0.706983 4.1:+80177014 None:intergenic
CTGGACCCAAGATCCCTCAA+CGG 0.709275 4.1:-80176188 MS.gene50804:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTAATTCTTTCAAATATAGA+AGG + chr4.1:80177020-80177039 None:intergenic 15.0%
!! ATTACATATTATTATGGTAC+AGG - chr4.1:80176751-80176770 MS.gene50804:CDS 20.0%
!! CCAAAATGTTAAAACATAAA+TGG + chr4.1:80176286-80176305 None:intergenic 20.0%
!! GTACCATAATAATATGTAAT+AGG + chr4.1:80176751-80176770 None:intergenic 20.0%
!!! AACATAAAAAAAGTTGATTC+TGG + chr4.1:80177287-80177306 None:intergenic 20.0%
!!! CCATTTATGTTTTAACATTT+TGG - chr4.1:80176283-80176302 MS.gene50804:CDS 20.0%
! CACACAAAAAATTTACTTGA+TGG + chr4.1:80175876-80175895 None:intergenic 25.0%
! CCACCTATTACATATTATTA+TGG - chr4.1:80176745-80176764 MS.gene50804:CDS 25.0%
! CCATAATAATATGTAATAGG+TGG + chr4.1:80176748-80176767 None:intergenic 25.0%
! CTATATATAAACTCTTGCTT+TGG + chr4.1:80176781-80176800 None:intergenic 25.0%
! GAAAATAGATGAATTACAGA+AGG + chr4.1:80176195-80176214 None:intergenic 25.0%
! TAATAATATGTAATAGGTGG+TGG + chr4.1:80176745-80176764 None:intergenic 25.0%
!! TAAACAAGAAACAACTCTTT+TGG - chr4.1:80177250-80177269 MS.gene50804:CDS 25.0%
!!! AAAAAAGTTGATTCTGGTTT+AGG + chr4.1:80177281-80177300 None:intergenic 25.0%
!!! AAAAGAGTTGTTTCTTGTTT+AGG + chr4.1:80177251-80177270 None:intergenic 25.0%
AAAGCCTACACAACAATTAA+AGG + chr4.1:80177158-80177177 None:intergenic 30.0%
AACAAGAGAAGTAAAGATAG+AGG - chr4.1:80176825-80176844 MS.gene50804:intron 30.0%
AAGCCTACACAACAATTAAA+GGG + chr4.1:80177157-80177176 None:intergenic 30.0%
ACAAGAGAAGTAAAGATAGA+GGG - chr4.1:80176826-80176845 MS.gene50804:intron 30.0%
ACAATTAAAGGGAATCAAAG+CGG + chr4.1:80177146-80177165 None:intergenic 30.0%
AGAAAATGGATCGTTGTTTA+TGG - chr4.1:80176025-80176044 MS.gene50804:CDS 30.0%
ATAATCTTTGCAGAAACCAA+TGG - chr4.1:80176496-80176515 MS.gene50804:CDS 30.0%
ATTCCCTTTAATTGTTGTGT+AGG - chr4.1:80177151-80177170 MS.gene50804:CDS 30.0%
GAAATCAACAAAATCTCCAT+TGG + chr4.1:80176515-80176534 None:intergenic 30.0%
GAATTACAGAAGGAAATACA+CGG + chr4.1:80176185-80176204 None:intergenic 30.0%
GCTACTTCTCAAAATTGTAT+TGG + chr4.1:80176359-80176378 None:intergenic 30.0%
TAATAGACAGCCTAAGAATT+TGG - chr4.1:80175956-80175975 MS.gene50804:intron 30.0%
! TTAGGAGCTAATAATTTTGC+TGG - chr4.1:80176580-80176599 MS.gene50804:CDS 30.0%
AATTTGGATTCTGATGGTCA+AGG - chr4.1:80175972-80175991 MS.gene50804:intron 35.0%
AGAGCAGCATTATATGGTAT+AGG - chr4.1:80176955-80176974 MS.gene50804:CDS 35.0%
AGATATACAACTGACCCTAA+TGG + chr4.1:80176559-80176578 None:intergenic 35.0%
ATGCAGCAATTGATGGAATT+GGG - chr4.1:80176092-80176111 MS.gene50804:CDS 35.0%
ATTCTTAGGCTGTCTATTAG+TGG + chr4.1:80175955-80175974 None:intergenic 35.0%
ATTTGGATTCTGATGGTCAA+GGG - chr4.1:80175973-80175992 MS.gene50804:intron 35.0%
CCATCAGAATCCAAATTCTT+AGG + chr4.1:80175969-80175988 None:intergenic 35.0%
CCTAAGAATTTGGATTCTGA+TGG - chr4.1:80175966-80175985 MS.gene50804:intron 35.0%
GTAAAGAGAGCAGCATTATA+TGG - chr4.1:80176949-80176968 MS.gene50804:CDS 35.0%
TCAAGATTTACCTTGACTAG+TGG - chr4.1:80177203-80177222 MS.gene50804:CDS 35.0%
TGCAAAATTCTTAACTGCAC+TGG - chr4.1:80176885-80176904 MS.gene50804:intron 35.0%
TTCAGACTTGACTCAAATAG+AGG - chr4.1:80176118-80176137 MS.gene50804:CDS 35.0%
TTCTTAGGCTGTCTATTAGT+GGG + chr4.1:80175954-80175973 None:intergenic 35.0%
! AATAATTTTGCTGGATGTGC+TGG - chr4.1:80176589-80176608 MS.gene50804:CDS 35.0%
! CTCAAAATTGTATTGGTGAG+AGG + chr4.1:80176352-80176371 None:intergenic 35.0%
! GGGTCAGTTGTATATCTTTT+AGG - chr4.1:80176562-80176581 MS.gene50804:CDS 35.0%
! TGGTAATTTGTACAAGAGTG+TGG - chr4.1:80176609-80176628 MS.gene50804:CDS 35.0%
!! AACGGATCACAAGTTGATAT+GGG - chr4.1:80176922-80176941 MS.gene50804:intron 35.0%
ACTGCTGATGCAAATTGGTT+AGG + chr4.1:80176403-80176422 None:intergenic 40.0%
AGAGAAGTAAAGATAGAGGG+AGG - chr4.1:80176829-80176848 MS.gene50804:intron 40.0%
AGGAAAGAATTAGCAGAGAG+TGG + chr4.1:80177000-80177019 None:intergenic 40.0%
ATCAACTTGTGATCCGTTGA+GGG + chr4.1:80176920-80176939 None:intergenic 40.0%
CAAGGGAATAATGCACAAGA+TGG - chr4.1:80175990-80176009 MS.gene50804:intron 40.0%
CAATTCCATCAATTGCTGCA+TGG + chr4.1:80176093-80176112 None:intergenic 40.0%
CATGCAGCAATTGATGGAAT+TGG - chr4.1:80176091-80176110 MS.gene50804:CDS 40.0%
CTCTAACTGCTGATGCAAAT+TGG + chr4.1:80176408-80176427 None:intergenic 40.0%
CTGATGCAAATTGGTTAGGT+GGG + chr4.1:80176399-80176418 None:intergenic 40.0%
CTGCATTTCAGGAATGTTGA+GGG + chr4.1:80176722-80176741 None:intergenic 40.0%
CTTCAAGATCGTCAAGAGAA+AGG + chr4.1:80177044-80177063 None:intergenic 40.0%
CTTGTTTAGGCACACTCAAA+AGG + chr4.1:80177238-80177257 None:intergenic 40.0%
GGAGTGATTTCTAAGTCACA+CGG - chr4.1:80176850-80176869 MS.gene50804:intron 40.0%
GGCGTCTTTATGACAGAAAA+TGG - chr4.1:80176011-80176030 MS.gene50804:intron 40.0%
GGGAGGTTAAGGAATAATTC+AGG + chr4.1:80176324-80176343 None:intergenic 40.0%
GTCTTTAAGTCCACTAGTCA+AGG + chr4.1:80177216-80177235 None:intergenic 40.0%
TATCAACTTGTGATCCGTTG+AGG + chr4.1:80176921-80176940 None:intergenic 40.0%
TCTTGACGATCTTGAAGCAA+AGG - chr4.1:80177047-80177066 MS.gene50804:CDS 40.0%
TGCAGCAATTGATGGAATTG+GGG - chr4.1:80176093-80176112 MS.gene50804:CDS 40.0%
TGCATTTCAGGAATGTTGAG+GGG + chr4.1:80176721-80176740 None:intergenic 40.0%
TTCATGCAAAGCGATTCACA+TGG + chr4.1:80175825-80175844 None:intergenic 40.0%
TTGATGTCAAGAATGCTCCA+AGG + chr4.1:80177187-80177206 None:intergenic 40.0%
! ATCCATTTTCCTGATGTCAC+CGG + chr4.1:80176443-80176462 None:intergenic 40.0%
!! CAACGGATCACAAGTTGATA+TGG - chr4.1:80176921-80176940 MS.gene50804:intron 40.0%
!!! TCTATTAGTGGGTTTTCCAC+AGG + chr4.1:80175943-80175962 None:intergenic 40.0%
AAGCGATTCACATGGATTCC+TGG + chr4.1:80175817-80175836 None:intergenic 45.0%
ACACAACATTGGCAAACAGG+AGG - chr4.1:80176148-80176167 MS.gene50804:CDS 45.0%
AGCGATTCACATGGATTCCT+GGG + chr4.1:80175816-80175835 None:intergenic 45.0%
AGCTTCCTTACGTTGCCATT+AGG - chr4.1:80176541-80176560 MS.gene50804:CDS 45.0%
ATGGAAGTCACAGAGGAACT+TGG + chr4.1:80176074-80176093 None:intergenic 45.0%
CACACACAACATTGGCAAAC+AGG - chr4.1:80176145-80176164 MS.gene50804:CDS 45.0%
CACAGGTACAGTGAAGGTTT+CGG + chr4.1:80175926-80175945 None:intergenic 45.0%
CATTGGCAAACAGGAGGTAA+TGG - chr4.1:80176154-80176173 MS.gene50804:CDS 45.0%
GAAGCAAAGGTGATGAGCAT+CGG - chr4.1:80177060-80177079 MS.gene50804:CDS 45.0%
GACTTCCATGCAGCAATTGA+TGG - chr4.1:80176085-80176104 MS.gene50804:CDS 45.0%
GAGGAAGTCACACACAACAT+TGG - chr4.1:80176137-80176156 MS.gene50804:CDS 45.0%
GCTGATGCAAATTGGTTAGG+TGG + chr4.1:80176400-80176419 None:intergenic 45.0%
GCTGCATTTCAGGAATGTTG+AGG + chr4.1:80176723-80176742 None:intergenic 45.0%
GCTTCCTTACGTTGCCATTA+GGG - chr4.1:80176542-80176561 MS.gene50804:CDS 45.0%
TGGAGAACTTGGACTCTTCA+TGG - chr4.1:80176629-80176648 MS.gene50804:CDS 45.0%
! GTACAAGAGTGTGGAGAACT+TGG - chr4.1:80176618-80176637 MS.gene50804:CDS 45.0%
! GTTTTCCACAGGTACAGTGA+AGG + chr4.1:80175932-80175951 None:intergenic 45.0%
! TGTATTGGTGAGAGGCTCAT+GGG + chr4.1:80176344-80176363 None:intergenic 45.0%
! TTGTATTGGTGAGAGGCTCA+TGG + chr4.1:80176345-80176364 None:intergenic 45.0%
CACCGGTGACATCAGGAAAA+TGG - chr4.1:80176438-80176457 MS.gene50804:CDS 50.0%
CGAAACCTTCACTGTACCTG+TGG - chr4.1:80175924-80175943 MS.gene50804:intron 50.0%
CTGACCCTAATGGCAACGTA+AGG + chr4.1:80176549-80176568 None:intergenic 50.0%
GTGATCCGTTGAGGGATCTT+GGG + chr4.1:80176912-80176931 None:intergenic 50.0%
GTTAGAGCAACGTCTTGCAC+CGG - chr4.1:80176421-80176440 MS.gene50804:CDS 50.0%
TGTGATCCGTTGAGGGATCT+TGG + chr4.1:80176913-80176932 None:intergenic 50.0%
TTGCTGCATGGAAGTCACAG+AGG + chr4.1:80176081-80176100 None:intergenic 50.0%
TTTCAGGAATGTTGAGGGGC+TGG + chr4.1:80176717-80176736 None:intergenic 50.0%
! ATAGGTGGTGGCTGCATTTC+AGG + chr4.1:80176733-80176752 None:intergenic 50.0%
! CGCACTGTGTTACTCAATCC+TGG - chr4.1:80176664-80176683 MS.gene50804:CDS 50.0%
! GCTGGTTTCGACAACCAAAC+TGG + chr4.1:80176699-80176718 None:intergenic 50.0%
!! AGGTGGGCTGTCTTTGTTCT+TGG + chr4.1:80176383-80176402 None:intergenic 50.0%
!! GTGTAGGCTTTGAGCTTCCT+TGG - chr4.1:80177167-80177186 MS.gene50804:CDS 50.0%
ATTGGTGAGAGGCTCATGGG+AGG + chr4.1:80176341-80176360 None:intergenic 55.0%
CCAAACTGGTGTGCTACACC+AGG + chr4.1:80176685-80176704 None:intergenic 55.0%
CCTGGTGTAGCACACCAGTT+TGG - chr4.1:80176682-80176701 MS.gene50804:CDS 55.0%
CTGGACCCAAGATCCCTCAA+CGG - chr4.1:80176904-80176923 MS.gene50804:intron 55.0%
GAGAGGCTCATGGGAGGTTA+AGG + chr4.1:80176335-80176354 None:intergenic 55.0%
!! GATGAGCATCGGTGAAGCTG+AGG - chr4.1:80177071-80177090 MS.gene50804:CDS 55.0%
! CGTCTTGCACCGGTGACATC+AGG - chr4.1:80176431-80176450 MS.gene50804:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80175801 80177313 80175801 ID=MS.gene50804
chr4.1 mRNA 80175801 80177313 80175801 ID=MS.gene50804.t1;Parent=MS.gene50804
chr4.1 exon 80176945 80177313 80176945 ID=MS.gene50804.t1.exon1;Parent=MS.gene50804.t1
chr4.1 CDS 80176945 80177313 80176945 ID=cds.MS.gene50804.t1;Parent=MS.gene50804.t1
chr4.1 exon 80176022 80176804 80176022 ID=MS.gene50804.t1.exon2;Parent=MS.gene50804.t1
chr4.1 CDS 80176022 80176804 80176022 ID=cds.MS.gene50804.t1;Parent=MS.gene50804.t1
chr4.1 exon 80175801 80175941 80175801 ID=MS.gene50804.t1.exon3;Parent=MS.gene50804.t1
chr4.1 CDS 80175801 80175941 80175801 ID=cds.MS.gene50804.t1;Parent=MS.gene50804.t1
Gene Sequence

>MS.gene50804

ATGTTGCTTCATCCCAGGAATCCATGTGAATCGCTTTGCATGAAGCTGTTCTTGAATGTTGATGACACTGAGCCATCAAGTAAATTTTTTGTGTGTGATTCTTGTAACACGTTTACTACTTTCCGAAACCTTCACTGTACCTGTGGAAAACCCACTAATAGACAGCCTAAGAATTTGGATTCTGATGGTCAAGGGAATAATGCACAAGATGGCGTCTTTATGACAGAAAATGGATCGTTGTTTATGGTGTCTGATGATTTGAAGATTGTGCCAAGTTCCTCTGTGACTTCCATGCAGCAATTGATGGAATTGGGGTATTCAGACTTGACTCAAATAGAGGAAGTCACACACAACATTGGCAAACAGGAGGTAATGGTGTTACCGTGTATTTCCTTCTGTAATTCATCTATTTTCAAACAATATTATAATTTCTTCTTTCTAAATTCGAGTTTGTATCAGTCTTTATTTAACTGTTATGCTAACCATTTATGTTTTAACATTTTGGATAGATATTGAAACTCCTGAATTATTCCTTAACCTCCCATGAGCCTCTCACCAATACAATTTTGAGAAGTAGCTCCAAGAACAAAGACAGCCCACCTAACCAATTTGCATCAGCAGTTAGAGCAACGTCTTGCACCGGTGACATCAGGAAAATGGATGTTAAAGTAATTCAATGTAAATCTCAGAAAAATATAATCTTTGCAGAAACCAATGGAGATTTTGTTGATTTCATTTTTAGCTTCCTTACGTTGCCATTAGGGTCAGTTGTATATCTTTTAGGAGCTAATAATTTTGCTGGATGTGCTGGTAATTTGTACAAGAGTGTGGAGAACTTGGACTCTTCATGGTGTACTGACTCACGCACTGTGTTACTCAATCCTGGTGTAGCACACCAGTTTGGTTGTCGAAACCAGCCCCTCAACATTCCTGAAATGCAGCCACCACCTATTACATATTATTATGGTACAGGAACACCAAAGCAAGAGTTTATATATAGTTATCATAGACGTGATTATTATGAAACAAGAGAAGTAAAGATAGAGGGAGGAGTGATTTCTAAGTCACACGGATCAATTTATAATGCAAAATTCTTAACTGCACTGGACCCAAGATCCCTCAACGGATCACAAGTTGATATGGGATTTGTAAAGAGAGCAGCATTATATGGTATAGGAAATGATCTAAGAGTAAAACCACTCTCTGCTAATTCTTTCCTTCTATATTTGAAAGAATTAAACCTTTCTCTTGACGATCTTGAAGCAAAGGTGATGAGCATCGGTGAAGCTGAGGTAATGTGCTATTATTTATCACTAAATATTTTCTTTTTTTGTCATTTCAGCCGCTTTGATTCCCTTTAATTGTTGTGTAGGCTTTGAGCTTCCTTGGAGCATTCTTGACATCAAGATTTACCTTGACTAGTGGACTTAAAGACCTTTTGAGTGTGCCTAAACAAGAAACAACTCTTTTGGATGTTCCTAAACCAGAATCAACTTTTTTTATGTTAATTTGA
Protein sequence

>MS.gene50804.t1

MLLHPRNPCESLCMKLFLNVDDTEPSSKFFVCDSCNTFTTFRNLHCTCGKPTNRQPKNLDSDGQGNNAQDGVFMTENGSLFMVSDDLKIVPSSSVTSMQQLMELGYSDLTQIEEVTHNIGKQEILKLLNYSLTSHEPLTNTILRSSSKNKDSPPNQFASAVRATSCTGDIRKMDVKVIQCKSQKNIIFAETNGDFVDFIFSFLTLPLGSVVYLLGANNFAGCAGNLYKSVENLDSSWCTDSRTVLLNPGVAHQFGCRNQPLNIPEMQPPPITYYYGTGTPKQEFIYSYHRRDYYETREVKIEGGVISKSHGSIYNAKFLTALDPRSLNGSQVDMGFVKRAALYGIGNDLRVKPLSANSFLLYLKELNLSLDDLEAKVMSIGEAEALSFLGAFLTSRFTLTSGLKDLLSVPKQETTLLDVPKPESTFFMLI