AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene50812

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50812.t1 MTR_4g017610 89.666 329 33 1 1 328 1 329 0.0 598
MS.gene50812.t1 MTR_4g017410 69.817 328 91 4 7 326 4 331 6.56e-157 449
MS.gene50812.t1 MTR_4g017830 61.890 328 122 2 1 327 1 326 1.48e-140 409
MS.gene50812.t1 MTR_4g017830 26.459 257 129 8 15 240 270 497 6.22e-12 67.0
MS.gene50812.t1 MTR_4g019340 64.134 329 105 5 11 328 37 363 3.11e-140 407
MS.gene50812.t1 MTR_4g019340 29.224 219 131 6 15 232 306 501 1.77e-13 71.6
MS.gene50812.t1 MTR_4g017440 64.478 335 109 4 1 327 1 333 3.91e-137 399
MS.gene50812.t1 MTR_4g017550 66.990 309 92 5 26 328 3 307 4.14e-136 395
MS.gene50812.t1 MTR_4g017550 27.016 248 131 9 15 240 250 469 1.15e-11 65.9
MS.gene50812.t1 MTR_4g017470 55.856 333 103 4 1 328 1 294 2.99e-114 340
MS.gene50812.t1 MTR_4g019310 40.181 331 177 10 8 327 5 325 2.20e-65 214
MS.gene50812.t1 MTR_7g066340 37.463 339 181 10 1 326 1 321 1.33e-62 207
MS.gene50812.t1 MTR_7g066340 28.814 236 126 8 5 240 256 449 3.20e-15 76.6
MS.gene50812.t1 MTR_7g066260 38.154 325 173 9 15 326 18 327 3.66e-61 203
MS.gene50812.t1 MTR_7g066350 38.080 323 174 7 15 326 12 319 8.08e-60 199
MS.gene50812.t1 MTR_7g058710 36.522 345 166 9 1 326 1 311 1.56e-56 191
MS.gene50812.t1 MTR_1g041315 37.425 334 173 12 15 326 17 336 2.27e-55 188
MS.gene50812.t1 MTR_7g066570 38.253 332 166 12 15 333 17 322 1.20e-54 188
MS.gene50812.t1 MTR_7g066570 28.099 242 145 6 9 240 254 476 1.01e-13 72.4
MS.gene50812.t1 MTR_7g012680 35.103 339 159 11 5 326 7 301 2.36e-52 179
MS.gene50812.t1 MTR_7g066290 37.963 324 173 9 15 326 18 325 1.61e-51 176
MS.gene50812.t1 MTR_7g066430 36.280 328 174 12 15 326 7 315 7.65e-49 171
MS.gene50812.t1 MTR_7g066430 24.797 246 159 4 6 240 251 481 5.54e-14 73.2
MS.gene50812.t1 MTR_7g063660 36.533 323 172 13 15 328 12 310 3.34e-48 169
MS.gene50812.t1 MTR_7g066410 34.320 338 180 11 19 326 17 342 2.15e-47 167
MS.gene50812.t1 MTR_7g066410 25.296 253 151 7 5 240 277 508 1.20e-11 65.9
MS.gene50812.t1 MTR_7g066360 35.443 316 177 10 3 306 5 305 1.22e-44 159
MS.gene50812.t1 MTR_4g019240 62.185 119 43 1 4 122 5 121 1.22e-44 151
MS.gene50812.t1 MTR_7g066400 32.743 339 174 10 1 326 1 298 6.04e-44 157
MS.gene50812.t1 MTR_7g063880 34.535 333 176 13 2 326 5 303 9.70e-44 157
MS.gene50812.t1 MTR_7g066580 33.962 318 154 10 19 326 2 273 1.10e-43 156
MS.gene50812.t1 MTR_7g066580 28.163 245 148 7 9 240 212 441 9.21e-12 66.2
MS.gene50812.t1 MTR_7g063820 37.092 337 176 14 1 326 1 312 4.31e-43 155
MS.gene50812.t1 MTR_7g063900 35.494 324 175 10 15 328 13 312 5.79e-43 155
MS.gene50812.t1 MTR_7g058700 33.828 337 144 12 18 326 525 810 7.47e-43 158
MS.gene50812.t1 MTR_7g063890 34.169 319 181 8 15 326 30 326 1.32e-42 154
MS.gene50812.t1 MTR_7g063830 34.675 323 177 12 15 326 13 312 3.06e-42 152
MS.gene50812.t1 MTR_7g062300 32.440 336 195 9 1 326 1 314 1.78e-40 148
MS.gene50812.t1 MTR_7g062280 33.143 350 184 13 1 326 1 324 4.14e-40 147
MS.gene50812.t1 MTR_7g063840 33.030 330 185 11 7 326 5 308 4.87e-40 147
MS.gene50812.t1 MTR_7g066480 35.404 322 179 11 15 326 19 321 5.14e-40 146
MS.gene50812.t1 MTR_7g066490 32.416 327 178 10 15 331 19 312 4.38e-37 138
MS.gene50812.t1 MTR_2g029400 34.894 235 96 3 8 242 10 187 9.07e-36 134
MS.gene50812.t1 MTR_7g066310 31.776 321 131 9 15 326 10 251 1.84e-34 130
MS.gene50812.t1 MTR_7g063920 31.307 329 168 10 2 328 5 277 1.36e-32 126
MS.gene50812.t1 MTR_7g063670 30.595 353 190 11 1 335 4 319 3.11e-32 125
MS.gene50812.t1 MTR_1g014060 35.242 227 124 6 21 240 9 219 7.03e-32 123
MS.gene50812.t1 MTR_3g007740 29.846 325 195 7 8 326 5 302 8.02e-31 121
MS.gene50812.t1 MTR_3g065200 31.650 297 146 11 15 306 6 250 2.37e-30 120
MS.gene50812.t1 MTR_7g066280 31.381 239 122 11 99 326 1 208 1.69e-21 94.7
MS.gene50812.t1 MTR_7g062270 36.735 196 94 9 7 192 5 180 1.78e-21 94.7
MS.gene50812.t1 MTR_8g023240 25.000 328 201 8 12 327 37 331 1.85e-18 86.7
MS.gene50812.t1 MTR_8g445860 27.642 246 145 6 10 242 11 236 1.44e-16 78.2
MS.gene50812.t1 MTR_6g006800 75.556 45 11 0 3 47 2 46 2.11e-15 70.5
MS.gene50812.t1 MTR_1g115440 27.426 237 150 6 15 242 14 237 4.72e-15 73.9
MS.gene50812.t1 MTR_1g115455 28.514 249 128 10 15 242 12 231 2.34e-13 68.9
MS.gene50812.t1 MTR_7g066680 36.134 119 64 2 215 326 31 144 1.52e-12 68.2
MS.gene50812.t1 MTR_0150s0010 29.167 240 142 8 15 242 13 236 2.86e-12 66.2
MS.gene50812.t1 MTR_1g115435 26.582 237 143 6 10 246 5 210 3.47e-12 65.5
MS.gene50812.t1 MTR_7g063720 25.641 234 133 8 13 240 24 222 4.60e-11 62.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene50812.t1 AT3G09110 33.841 328 193 9 9 328 6 317 2.17e-48 166
MS.gene50812.t1 AT3G09140 32.464 345 202 9 6 328 3 338 3.18e-45 161
MS.gene50812.t1 AT3G09120 34.337 332 179 13 9 322 3 313 9.68e-40 142
MS.gene50812.t1 AT3G09140 30.027 373 202 10 6 328 3 366 1.71e-39 146
MS.gene50812.t1 AT5G43240 33.657 309 172 11 15 305 12 305 7.71e-39 144
MS.gene50812.t1 AT5G43240 33.657 309 172 11 15 305 12 305 7.71e-39 144
MS.gene50812.t1 AT5G43240 33.657 309 172 11 15 305 12 305 7.71e-39 144
MS.gene50812.t1 AT5G01120 30.514 331 210 7 9 328 6 327 1.97e-38 143
MS.gene50812.t1 AT5G01150 30.347 346 193 10 6 328 3 323 5.14e-38 142
MS.gene50812.t1 AT5G01130 31.045 335 194 11 6 328 3 312 3.42e-36 136
MS.gene50812.t1 AT5G01140 31.722 331 185 10 6 328 3 300 6.68e-35 133
MS.gene50812.t1 AT5G37320 31.722 331 196 13 19 330 18 337 9.33e-32 124

Find 65 sgRNAs with CRISPR-Local

Find 115 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGTTTCCTTACCATTCCATT+AGG 0.251119 4.1:-80100708 MS.gene50812:CDS
TAATAGACAACCTAAGAATT+TGG 0.262420 4.1:-80101655 MS.gene50812:CDS
CAATTATAAGATAAATAAAA+TGG 0.268027 4.1:-80100811 MS.gene50812:CDS
CAGGGGAATCATGCCTCTAA+TGG 0.286513 4.1:-80101621 MS.gene50812:CDS
CGTCGTCTGAGTTGAGGTTT+TGG 0.288022 4.1:+80101859 None:intergenic
GGGTATTAAAGTCTTTATAA+TGG 0.290144 4.1:+80100683 None:intergenic
CCCTCAGAGTCCAAATTCTT+AGG 0.309465 4.1:+80101645 None:intergenic
AAATTCTTAGGTTGTCTATT+AGG 0.310647 4.1:+80101657 None:intergenic
ATTCTTCTCCATCAACATAT+TGG 0.310876 4.1:+80100838 None:intergenic
TCAGAATCATCACCATTCTT+TGG 0.319703 4.1:-80101909 MS.gene50812:CDS
ATGCAGATGTTGCTGGAATC+AGG 0.320991 4.1:-80101519 MS.gene50812:CDS
TCCAATATTGTATTCGTAAG+AGG 0.327753 4.1:+80100894 None:intergenic
AGCAACATTTGCTTACATAA+AGG 0.329982 4.1:+80101825 None:intergenic
TGTTGTTGGTGGTTGCTAAA+AGG 0.334739 4.1:+80101949 None:intergenic
GCCTCTTACGAATACAATAT+TGG 0.339705 4.1:-80100895 MS.gene50812:CDS
GTTTCCTTACCATTCCATTA+GGG 0.340279 4.1:-80100707 MS.gene50812:CDS
GCAACATTTGCTTACATAAA+GGG 0.341137 4.1:+80101826 None:intergenic
TGTATCAAACACCCCATTAG+AGG 0.344943 4.1:+80101608 None:intergenic
ACCTCAACTCAGACGACGTT+TGG 0.355641 4.1:-80101854 MS.gene50812:CDS
ATATGTTGATGGAGAAGAAT+TGG 0.357603 4.1:-80100835 MS.gene50812:CDS
CAGACATAAAACTTACTTGA+TGG 0.362318 4.1:+80101738 None:intergenic
AGGGGAATCATGCCTCTAAT+GGG 0.380156 4.1:-80101620 MS.gene50812:CDS
GAACTCAATGCAGATGTTGC+TGG 0.386915 4.1:-80101526 MS.gene50812:CDS
GAATTTGGACTCTGAGGGTC+AGG 0.397162 4.1:-80101640 MS.gene50812:CDS
GTCTTTATAATGGACCCTAA+TGG 0.418519 4.1:+80100693 None:intergenic
AGCTTCCTATCTTTGCCACT+TGG 0.441332 4.1:-80101984 MS.gene50812:CDS
AAGAGGCTCATGAGAGGTTA+AGG 0.445820 4.1:+80100911 None:intergenic
TGGTACCAAGTGGCAAAGAT+AGG 0.458873 4.1:+80101979 None:intergenic
GAGAGGTTAAGGTATACTTC+AGG 0.468779 4.1:+80100922 None:intergenic
TATGTTGATGGAGAAGAATT+GGG 0.477595 4.1:-80100834 MS.gene50812:CDS
CCACGGAACCAATATGTTGA+TGG 0.488534 4.1:-80100846 MS.gene50812:CDS
TTGTCGATCATTGTTGTTGG+TGG 0.499724 4.1:+80101938 None:intergenic
ATTGAGTTCAGCAAGGAACT+TGG 0.501314 4.1:+80101540 None:intergenic
AACACATCCAGGAAAATTGT+TGG 0.509207 4.1:+80100662 None:intergenic
CACACAAAGCATTGGCAAGA+AGG 0.511399 4.1:-80101466 MS.gene50812:CDS
CGAAACCTTGACTGCACCTG+CGG 0.512942 4.1:-80101687 MS.gene50812:CDS
TTCTTAGGTTGTCTATTAGG+AGG 0.515531 4.1:+80101660 None:intergenic
CCATCAACATATTGGTTCCG+TGG 0.536459 4.1:+80100846 None:intergenic
TCCAAACGTCGTCTGAGTTG+AGG 0.536465 4.1:+80101853 None:intergenic
TGCAGATGTTGCTGGAATCA+GGG 0.536790 4.1:-80101518 MS.gene50812:CDS
TGTTTGATGTTGCCAAAGAA+TGG 0.539653 4.1:+80101897 None:intergenic
TACAAATTACCAACACATCC+AGG 0.541284 4.1:+80100651 None:intergenic
ACACATCCAGGAAAATTGTT+GGG 0.541913 4.1:+80100663 None:intergenic
ATCATCATTGCAGAAGCTGA+TGG 0.549559 4.1:-80100753 MS.gene50812:CDS
ATTCATGTGAATCACTATGC+AGG 0.552422 4.1:-80101791 MS.gene50812:CDS
TTGTTGTTGTTGAGAAGTCA+TGG 0.559710 4.1:+80102106 None:intergenic
ACCTAAGAATTTGGACTCTG+AGG 0.575501 4.1:-80101646 MS.gene50812:CDS
AGTGATTCACATGAATTCCT+CGG 0.577391 4.1:+80101798 None:intergenic
TGGACCCTAATGGAATGGTA+AGG 0.578424 4.1:+80100703 None:intergenic
TATAATGGACCCTAATGGAA+TGG 0.581198 4.1:+80100698 None:intergenic
AGCAAATGTTGCTGCATCCG+AGG 0.591888 4.1:-80101815 MS.gene50812:CDS
CCTAAGAATTTGGACTCTGA+GGG 0.592717 4.1:-80101645 MS.gene50812:CDS
AATTTGGACTCTGAGGGTCA+GGG 0.595588 4.1:-80101639 MS.gene50812:CDS
ATTTGGACTCTGAGGGTCAG+GGG 0.598326 4.1:-80101638 MS.gene50812:CDS
GAGGAAGTCACACAAAGCAT+TGG 0.599245 4.1:-80101474 MS.gene50812:CDS
AGGTTAAGGTATACTTCAGG+AGG 0.621580 4.1:+80100925 None:intergenic
TTCAGACTCAACGCAACTAG+AGG 0.625063 4.1:-80101493 MS.gene50812:CDS
AACGTTTCCCCTCAAAATCA+CGG 0.627379 4.1:-80102072 MS.gene50812:CDS
GGGGAATCATGCCTCTAATG+GGG 0.639572 4.1:-80101619 MS.gene50812:CDS
CAAAGAACAATGACGAACCA+CGG 0.642115 4.1:-80100863 MS.gene50812:CDS
ACAAAGCATTGGCAAGAAGG+AGG 0.647102 4.1:-80101463 MS.gene50812:intron
GGTTGCTAAAAGGCGAACGA+TGG 0.680679 4.1:+80101959 None:intergenic
ATTCGTAAGAGGCTCATGAG+AGG 0.692406 4.1:+80100905 None:intergenic
CATCTGCATTGAGTTCAGCA+AGG 0.698278 4.1:+80101533 None:intergenic
AGGCGAACGATGGTACCAAG+TGG 0.729828 4.1:+80101969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAATTATAAGATAAATAAAA+TGG - chr4.1:80101889-80101908 MS.gene50812:CDS 10.0%
!! ATAAAATGTTAAAACACAAA+TGG + chr4.1:80101743-80101762 None:intergenic 15.0%
!!! AAATTTAAAATCATAGGTTA+AGG + chr4.1:80101381-80101400 None:intergenic 15.0%
!!! TAACCTATGATTTTAAATTT+AGG - chr4.1:80101381-80101400 MS.gene50812:intron 15.0%
!! AATAAAGAGAAATAGAACTT+TGG + chr4.1:80101674-80101693 None:intergenic 20.0%
!! GGATATATATAGTTAATACT+TGG - chr4.1:80101523-80101542 MS.gene50812:CDS 20.0%
!!! GATTTTATTGACTTCATTTT+TGG - chr4.1:80101971-80101990 MS.gene50812:CDS 20.0%
! AAGAACACTACTAGAAAAAT+CGG - chr4.1:80102082-80102101 MS.gene50812:CDS 25.0%
! GGGTATTAAAGTCTTTATAA+TGG + chr4.1:80102020-80102039 None:intergenic 25.0%
! TAATAGACAACCTAAGAATT+TGG - chr4.1:80101045-80101064 MS.gene50812:intron 25.0%
! TTTCGTTGTGAATCTATATT+GGG - chr4.1:80101409-80101428 MS.gene50812:intron 25.0%
!! AAATTCTTAGGTTGTCTATT+AGG + chr4.1:80101046-80101065 None:intergenic 25.0%
!! GCACCTAAATTTAAAATCAT+AGG + chr4.1:80101387-80101406 None:intergenic 25.0%
!! TATCTAAAACCATTACATTG+TGG - chr4.1:80101479-80101498 MS.gene50812:CDS 25.0%
AAGAGAAATAGAACTTTGGT+AGG + chr4.1:80101670-80101689 None:intergenic 30.0%
AGAGAAATAGAACTTTGGTA+GGG + chr4.1:80101669-80101688 None:intergenic 30.0%
AGCAACATTTGCTTACATAA+AGG + chr4.1:80100878-80100897 None:intergenic 30.0%
AGTATGATGTTATCTGTTAG+TGG - chr4.1:80101555-80101574 MS.gene50812:CDS 30.0%
ATCTATATTGGGGTAAAATG+TGG - chr4.1:80101420-80101439 MS.gene50812:intron 30.0%
ATTCTTCTCCATCAACATAT+TGG + chr4.1:80101865-80101884 None:intergenic 30.0%
CAAATGGTTAGAATGAAAGT+TGG + chr4.1:80101727-80101746 None:intergenic 30.0%
CAGACATAAAACTTACTTGA+TGG + chr4.1:80100965-80100984 None:intergenic 30.0%
CTGCAATGACAATATTTGTT+AGG - chr4.1:80101502-80101521 MS.gene50812:CDS 30.0%
GCAACATTTGCTTACATAAA+GGG + chr4.1:80100877-80100896 None:intergenic 30.0%
GTTTCGTTGTGAATCTATAT+TGG - chr4.1:80101408-80101427 MS.gene50812:intron 30.0%
TCCAATATTGTATTCGTAAG+AGG + chr4.1:80101809-80101828 None:intergenic 30.0%
TGGAAAAAAACTCAATGGAA+CGG + chr4.1:80101322-80101341 None:intergenic 30.0%
TTCGTTGTGAATCTATATTG+GGG - chr4.1:80101410-80101429 MS.gene50812:intron 30.0%
! ATATGTTGATGGAGAAGAAT+TGG - chr4.1:80101865-80101884 MS.gene50812:CDS 30.0%
! TATGTTGATGGAGAAGAATT+GGG - chr4.1:80101866-80101885 MS.gene50812:CDS 30.0%
! TTAATACCCAACAATTTTCC+TGG - chr4.1:80102031-80102050 MS.gene50812:CDS 30.0%
! TTTTTGTCGATCATTGTTGT+TGG + chr4.1:80100768-80100787 None:intergenic 30.0%
!!! AAGCAACAAAGTTGTTTTTG+TGG - chr4.1:80100661-80100680 MS.gene50812:CDS 30.0%
!!! GTTTGTTGTGTTTCCATTTT+AGG - chr4.1:80101285-80101304 MS.gene50812:intron 30.0%
AACACATCCAGGAAAATTGT+TGG + chr4.1:80102041-80102060 None:intergenic 35.0%
ACACATCCAGGAAAATTGTT+GGG + chr4.1:80102040-80102059 None:intergenic 35.0%
AGTGATTCACATGAATTCCT+CGG + chr4.1:80100905-80100924 None:intergenic 35.0%
ATGTTATCTGTTAGTGGTAG+AGG - chr4.1:80101561-80101580 MS.gene50812:CDS 35.0%
ATTCATGTGAATCACTATGC+AGG - chr4.1:80100909-80100928 MS.gene50812:CDS 35.0%
GCAAGTGGAAAAAAACTCAA+TGG + chr4.1:80101327-80101346 None:intergenic 35.0%
GCCTCTTACGAATACAATAT+TGG - chr4.1:80101805-80101824 MS.gene50812:CDS 35.0%
GGAAAAAAACTCAATGGAAC+GGG + chr4.1:80101321-80101340 None:intergenic 35.0%
GTTTCCTTACCATTCCATTA+GGG - chr4.1:80101993-80102012 MS.gene50812:CDS 35.0%
TACAAATTACCAACACATCC+AGG + chr4.1:80102052-80102071 None:intergenic 35.0%
TCAGAATCATCACCATTCTT+TGG - chr4.1:80100791-80100810 MS.gene50812:CDS 35.0%
TGTTTGATGTTGCCAAAGAA+TGG + chr4.1:80100806-80100825 None:intergenic 35.0%
! AACAATTTTCCTGGATGTGT+TGG - chr4.1:80102040-80102059 MS.gene50812:CDS 35.0%
! AGAAAAATCGGATTTTGCGA+CGG - chr4.1:80102094-80102113 MS.gene50812:CDS 35.0%
! TTCTTAGGTTGTCTATTAGG+AGG + chr4.1:80101043-80101062 None:intergenic 35.0%
! TTTGTCTACCGTGATTTTGA+GGG + chr4.1:80100639-80100658 None:intergenic 35.0%
!! CTTGTTTCTACAATGACCTA+AGG + chr4.1:80101356-80101375 None:intergenic 35.0%
!! GTCTTTATAATGGACCCTAA+TGG + chr4.1:80102010-80102029 None:intergenic 35.0%
!! TATAATGGACCCTAATGGAA+TGG + chr4.1:80102005-80102024 None:intergenic 35.0%
AACGTTTCCCCTCAAAATCA+CGG - chr4.1:80100628-80100647 MS.gene50812:CDS 40.0%
ACCTAAGAATTTGGACTCTG+AGG - chr4.1:80101054-80101073 MS.gene50812:intron 40.0%
AGGTTAAGGTATACTTCAGG+AGG + chr4.1:80101778-80101797 None:intergenic 40.0%
AGTTAGAACTTAGAGGAGAC+TGG - chr4.1:80101604-80101623 MS.gene50812:CDS 40.0%
ATCATCATTGCAGAAGCTGA+TGG - chr4.1:80101947-80101966 MS.gene50812:CDS 40.0%
ATTGAGTTCAGCAAGGAACT+TGG + chr4.1:80101163-80101182 None:intergenic 40.0%
CAAAGAACAATGACGAACCA+CGG - chr4.1:80101837-80101856 MS.gene50812:CDS 40.0%
CCTAAGAATTTGGACTCTGA+GGG - chr4.1:80101055-80101074 MS.gene50812:intron 40.0%
GAAATAGAACTTTGGTAGGG+TGG + chr4.1:80101666-80101685 None:intergenic 40.0%
GAGAGGTTAAGGTATACTTC+AGG + chr4.1:80101781-80101800 None:intergenic 40.0%
GGGAACTAGTTAGAACTTAG+AGG - chr4.1:80101597-80101616 MS.gene50812:CDS 40.0%
GGTTTCCTTACCATTCCATT+AGG - chr4.1:80101992-80102011 MS.gene50812:CDS 40.0%
TACCTCGACAACGACATTAA+TGG + chr4.1:80101461-80101480 None:intergenic 40.0%
TCATTGCAGCCACAATGTAA+TGG + chr4.1:80101491-80101510 None:intergenic 40.0%
TGTATCAAACACCCCATTAG+AGG + chr4.1:80101095-80101114 None:intergenic 40.0%
TTCCACTTGCAAATGACCTT+AGG - chr4.1:80101337-80101356 MS.gene50812:intron 40.0%
! GTTTGTCTACCGTGATTTTG+AGG + chr4.1:80100640-80100659 None:intergenic 40.0%
! TTGTCGATCATTGTTGTTGG+TGG + chr4.1:80100765-80100784 None:intergenic 40.0%
! TTGTCTACCGTGATTTTGAG+GGG + chr4.1:80100638-80100657 None:intergenic 40.0%
!! GTAGAGGTGTCATTAGGAAA+GGG - chr4.1:80101577-80101596 MS.gene50812:CDS 40.0%
!! TGTTGTTGGTGGTTGCTAAA+AGG + chr4.1:80100754-80100773 None:intergenic 40.0%
!! TTAGTGGTAGAGGTGTCATT+AGG - chr4.1:80101571-80101590 MS.gene50812:CDS 40.0%
!!! AGTTGAGGTTTTGGACAGTT+TGG + chr4.1:80100835-80100854 None:intergenic 40.0%
!!! GGTTTTGGACAGTTTGGTAA+AGG + chr4.1:80100829-80100848 None:intergenic 40.0%
AAGAGGCTCATGAGAGGTTA+AGG + chr4.1:80101792-80101811 None:intergenic 45.0%
ACTTTGGTAGGGTGGAACAA+TGG + chr4.1:80101658-80101677 None:intergenic 45.0%
AGCTTCCTATCTTTGCCACT+TGG - chr4.1:80100716-80100735 MS.gene50812:CDS 45.0%
AGGGGAATCATGCCTCTAAT+GGG - chr4.1:80101080-80101099 MS.gene50812:intron 45.0%
ATGCAGATGTTGCTGGAATC+AGG - chr4.1:80101181-80101200 MS.gene50812:intron 45.0%
ATTCGTAAGAGGCTCATGAG+AGG + chr4.1:80101798-80101817 None:intergenic 45.0%
CATCTGCATTGAGTTCAGCA+AGG + chr4.1:80101170-80101189 None:intergenic 45.0%
CCACGGAACCAATATGTTGA+TGG - chr4.1:80101854-80101873 MS.gene50812:CDS 45.0%
CCCTCAGAGTCCAAATTCTT+AGG + chr4.1:80101058-80101077 None:intergenic 45.0%
GAACTCAATGCAGATGTTGC+TGG - chr4.1:80101174-80101193 MS.gene50812:intron 45.0%
GACCTAAGGTCATTTGCAAG+TGG + chr4.1:80101342-80101361 None:intergenic 45.0%
GAGGAAGTCACACAAAGCAT+TGG - chr4.1:80101226-80101245 MS.gene50812:intron 45.0%
GGGTGCATAATGGCCTAAAA+TGG + chr4.1:80101301-80101320 None:intergenic 45.0%
TCAATGGAACGGGTGCATAA+TGG + chr4.1:80101311-80101330 None:intergenic 45.0%
TGCAGATGTTGCTGGAATCA+GGG - chr4.1:80101182-80101201 MS.gene50812:intron 45.0%
TGCCATTAATGTCGTTGTCG+AGG - chr4.1:80101456-80101475 MS.gene50812:intron 45.0%
TGGACCCTAATGGAATGGTA+AGG + chr4.1:80102000-80102019 None:intergenic 45.0%
TGGCAAGAAGGAGGTAACTA+AGG - chr4.1:80101246-80101265 MS.gene50812:intron 45.0%
TGGTACCAAGTGGCAAAGAT+AGG + chr4.1:80100724-80100743 None:intergenic 45.0%
TTCAGACTCAACGCAACTAG+AGG - chr4.1:80101207-80101226 MS.gene50812:intron 45.0%
! AATTTGGACTCTGAGGGTCA+GGG - chr4.1:80101061-80101080 MS.gene50812:intron 45.0%
! TCTATTAGGAGGTTTTCCGC+AGG + chr4.1:80101032-80101051 None:intergenic 45.0%
!! ACAAAGCATTGGCAAGAAGG+AGG - chr4.1:80101237-80101256 MS.gene50812:intron 45.0%
!! CACACAAAGCATTGGCAAGA+AGG - chr4.1:80101234-80101253 MS.gene50812:intron 45.0%
!! CCATCAACATATTGGTTCCG+TGG + chr4.1:80101857-80101876 None:intergenic 45.0%
!! GGTAGAGGTGTCATTAGGAA+AGG - chr4.1:80101576-80101595 MS.gene50812:CDS 45.0%
ACCTCAACTCAGACGACGTT+TGG - chr4.1:80100846-80100865 MS.gene50812:CDS 50.0%
AGCAAATGTTGCTGCATCCG+AGG - chr4.1:80100885-80100904 MS.gene50812:CDS 50.0%
CAGGGGAATCATGCCTCTAA+TGG - chr4.1:80101079-80101098 MS.gene50812:intron 50.0%
GGGGAATCATGCCTCTAATG+GGG - chr4.1:80101081-80101100 MS.gene50812:intron 50.0%
GGTTGCTAAAAGGCGAACGA+TGG + chr4.1:80100744-80100763 None:intergenic 50.0%
TCCAAACGTCGTCTGAGTTG+AGG + chr4.1:80100850-80100869 None:intergenic 50.0%
! ATTTGGACTCTGAGGGTCAG+GGG - chr4.1:80101062-80101081 MS.gene50812:intron 50.0%
! GAATTTGGACTCTGAGGGTC+AGG - chr4.1:80101060-80101079 MS.gene50812:intron 50.0%
!! CGTCGTCTGAGTTGAGGTTT+TGG + chr4.1:80100844-80100863 None:intergenic 50.0%
AGGCGAACGATGGTACCAAG+TGG + chr4.1:80100734-80100753 None:intergenic 55.0%
CGAAACCTTGACTGCACCTG+CGG - chr4.1:80101013-80101032 MS.gene50812:intron 55.0%
! GTTTTCCGCAGGTGCAGTCA+AGG + chr4.1:80101021-80101040 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 80100596 80102126 80100596 ID=MS.gene50812
chr4.1 mRNA 80100596 80102126 80100596 ID=MS.gene50812.t1;Parent=MS.gene50812
chr4.1 exon 80101464 80102126 80101464 ID=MS.gene50812.t1.exon1;Parent=MS.gene50812.t1
chr4.1 CDS 80101464 80102126 80101464 ID=cds.MS.gene50812.t1;Parent=MS.gene50812.t1
chr4.1 exon 80100596 80100955 80100596 ID=MS.gene50812.t1.exon2;Parent=MS.gene50812.t1
chr4.1 CDS 80100596 80100955 80100596 ID=cds.MS.gene50812.t1;Parent=MS.gene50812.t1
Gene Sequence

>MS.gene50812

ATGACTTCTCAACAACAACAACAAGAGTCGAAAACGTTTCCCCTCAAAATCACGGTAGACAAACAAAGCAACAAAGTTGTTTTTGTGGAAGCAACAAAAGACTTTGTTGAGACTCTTTTCAGCTTCCTATCTTTGCCACTTGGTACCATCGTTCGCCTTTTAGCAACCACCAACAACAATGATCGACAAAAACTCTCAGAATCATCACCATTCTTTGGCAACATCAAACACCTTTACCAAACTGTCCAAAACCTCAACTCAGACGACGTTTGGAACAACCCTTTATGTAAGCAAATGTTGCTGCATCCGAGGAATTCATGTGAATCACTATGCAGGAAGTTGTACTTGAATATTGATGATTCTGAACCATCAAGTAAGTTTTATGTCTGTGATTCTTGTAACAAGTTCACTACTTTTCGAAACCTTGACTGCACCTGCGGAAAACCTCCTAATAGACAACCTAAGAATTTGGACTCTGAGGGTCAGGGGAATCATGCCTCTAATGGGGTGTTTGATACAGAAAGTGAACAAATGTTTCTAGTGTCTGATGATTTGAATATTGTGCCAAGTTCCTTGCTGAACTCAATGCAGATGTTGCTGGAATCAGGGATTTCAGACTCAACGCAACTAGAGGAAGTCACACAAAGCATTGGCAAGAAGGAGGTAACTAAGGATGTTGTGTTGTTTTTGTTTGTTGTGTTTCCATTTTAGGCCATTATGCACCCGTTCCATTGAGTTTTTTTCCACTTGCAAATGACCTTAGGTCATTGTAGAAACAAGATCCTTAACCTATGATTTTAAATTTAGGTGCAGTTTCGTTGTGAATCTATATTGGGGTAAAATGTGGTCGTATTTGCAGTTGCCATTAATGTCGTTGTCGAGGTATCTAAAACCATTACATTGTGGCTGCAATGACAATATTTGTTAGGATATATATAGTTAATACTTGGATTTGACATAGTATGATGTTATCTGTTAGTGGTAGAGGTGTCATTAGGAAAGGGAACTAGTTAGAACTTAGAGGAGACTGGTAAATATACAATTTACTCTTGTTGTGTTCCATTGTTCCACCCTACCAAAGTTCTATTTCTCTTTATTCAAGACTCTCTCAAGTATAATTTTTAAACTCCAACTTTCATTCTAACCATTTGTGTTTTAACATTTTATACAGATATTGAACCTCCTGAAGTATACCTTAACCTCTCATGAGCCTCTTACGAATACAATATTGGAAAGTAGCTCAAAGAACAATGACGAACCACGGAACCAATATGTTGATGGAGAAGAATTGGGCAATTATAAGATAAATAAAATGGATATTAAAGTATTGCAAAGCAAATCTCAGAATAAGATCATCATTGCAGAAGCTGATGGAGATTTTATTGACTTCATTTTTGGTTTCCTTACCATTCCATTAGGGTCCATTATAAAGACTTTAATACCCAACAATTTTCCTGGATGTGTTGGTAATTTGTACAACAGTGTGAAGAACACTACTAGAAAAATCGGATTTTGCGACGGAAATTTGTGA
Protein sequence

>MS.gene50812.t1

MTSQQQQQESKTFPLKITVDKQSNKVVFVEATKDFVETLFSFLSLPLGTIVRLLATTNNNDRQKLSESSPFFGNIKHLYQTVQNLNSDDVWNNPLCKQMLLHPRNSCESLCRKLYLNIDDSEPSSKFYVCDSCNKFTTFRNLDCTCGKPPNRQPKNLDSEGQGNHASNGVFDTESEQMFLVSDDLNIVPSSLLNSMQMLLESGISDSTQLEEVTQSIGKKEILNLLKYTLTSHEPLTNTILESSSKNNDEPRNQYVDGEELGNYKINKMDIKVLQSKSQNKIIIAEADGDFIDFIFGFLTIPLGSIIKTLIPNNFPGCVGNLYNSVKNTTRKIGFCDGNL