AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene037538

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037538.t1 MTR_4g017440 83.271 269 43 1 1 267 240 508 4.80e-157 447
MS.gene037538.t1 MTR_4g017470 63.899 277 90 4 1 267 200 476 5.49e-112 332
MS.gene037538.t1 MTR_4g017470 36.937 111 53 2 38 131 15 125 2.70e-11 63.5
MS.gene037538.t1 MTR_4g017830 63.273 275 92 4 1 267 234 507 1.55e-108 324
MS.gene037538.t1 MTR_4g017830 26.378 254 154 7 20 257 2 238 8.96e-12 65.1
MS.gene037538.t1 MTR_4g017550 61.798 267 84 7 1 257 211 469 2.01e-91 278
MS.gene037538.t1 MTR_4g019340 60.700 257 72 5 1 251 270 503 2.70e-91 279
MS.gene037538.t1 MTR_4g017410 58.065 279 78 6 1 267 239 490 5.30e-90 276
MS.gene037538.t1 MTR_4g017410 27.686 242 139 9 38 255 12 241 5.30e-12 65.9
MS.gene037538.t1 MTR_4g017610 51.154 260 105 6 1 258 237 476 4.51e-71 226
MS.gene037538.t1 MTR_4g017610 31.298 131 73 3 38 153 16 144 6.73e-11 62.4
MS.gene037538.t1 MTR_4g019310 39.764 254 140 7 20 267 251 497 1.13e-43 155
MS.gene037538.t1 MTR_4g019310 29.787 188 110 5 35 207 8 188 2.06e-12 67.0
MS.gene037538.t1 MTR_4g019350 55.556 90 34 1 93 182 1 84 5.53e-26 98.2
MS.gene037538.t1 MTR_7g066400 35.169 236 113 7 27 257 233 433 5.69e-25 103
MS.gene037538.t1 MTR_7g066340 32.618 233 111 5 35 262 263 454 2.50e-24 101
MS.gene037538.t1 MTR_7g066340 31.019 216 119 8 35 237 12 210 6.33e-15 74.3
MS.gene037538.t1 MTR_7g066430 34.016 244 137 8 33 263 255 487 6.26e-24 100
MS.gene037538.t1 MTR_7g066350 31.797 217 102 4 27 240 253 426 8.73e-24 100
MS.gene037538.t1 MTR_7g066570 33.613 238 141 5 35 265 257 484 4.54e-23 98.6
MS.gene037538.t1 MTR_7g058710 31.048 248 155 6 27 267 245 483 5.94e-23 97.8
MS.gene037538.t1 MTR_7g058710 27.586 232 144 9 30 254 7 221 5.52e-12 65.9
MS.gene037538.t1 MTR_7g066310 32.618 233 111 5 35 262 193 384 7.47e-23 97.1
MS.gene037538.t1 MTR_7g066310 32.484 157 89 5 35 177 7 160 7.35e-12 65.1
MS.gene037538.t1 MTR_7g066680 34.906 212 117 8 33 237 84 281 1.77e-22 95.1
MS.gene037538.t1 MTR_7g066280 31.967 244 142 6 38 262 153 391 4.07e-22 95.1
MS.gene037538.t1 MTR_1g041315 30.364 247 122 6 1 240 241 444 1.80e-20 90.9
MS.gene037538.t1 MTR_7g066410 33.476 233 145 5 35 261 284 512 4.44e-20 89.7
MS.gene037538.t1 MTR_7g066260 33.475 236 114 7 32 262 266 463 2.73e-19 87.4
MS.gene037538.t1 MTR_7g066260 32.447 188 94 9 33 205 13 182 8.40e-13 68.2
MS.gene037538.t1 MTR_7g066580 30.638 235 147 8 35 261 215 441 2.95e-19 87.0
MS.gene037538.t1 MTR_7g063900 29.204 226 148 4 19 237 236 456 7.43e-19 85.9
MS.gene037538.t1 MTR_7g063900 36.190 105 58 3 35 131 10 113 3.66e-12 66.2
MS.gene037538.t1 MTR_8g445860 28.175 252 126 8 31 255 9 232 1.15e-18 82.8
MS.gene037538.t1 MTR_7g062280 30.672 238 148 6 35 267 266 491 2.25e-18 84.7
MS.gene037538.t1 MTR_7g062280 34.483 174 94 6 48 208 23 189 1.33e-12 67.8
MS.gene037538.t1 MTR_7g063720 28.814 236 135 7 31 261 19 226 8.41e-18 80.5
MS.gene037538.t1 MTR_7g062300 29.237 236 146 7 31 260 252 472 2.05e-17 81.6
MS.gene037538.t1 MTR_7g062300 33.854 192 95 8 30 208 7 179 4.01e-15 75.1
MS.gene037538.t1 MTR_7g012680 31.513 238 129 6 35 267 243 451 3.83e-17 80.9
MS.gene037538.t1 MTR_7g012680 28.704 216 131 8 35 243 14 213 4.65e-12 65.9
MS.gene037538.t1 MTR_7g063660 32.051 234 141 7 40 267 256 477 1.67e-16 79.0
MS.gene037538.t1 MTR_7g063660 37.963 108 60 2 35 135 9 116 8.33e-15 73.9
MS.gene037538.t1 MTR_7g063920 28.631 241 149 5 32 267 214 436 6.95e-16 77.0
MS.gene037538.t1 MTR_7g063920 38.182 110 59 3 30 131 6 114 1.15e-11 64.7
MS.gene037538.t1 MTR_1g115450 41.176 85 48 1 31 113 3 87 3.79e-15 70.9
MS.gene037538.t1 MTR_7g063890 30.500 200 127 5 45 237 278 472 5.31e-15 74.7
MS.gene037538.t1 MTR_7g063890 29.944 177 98 7 38 205 30 189 9.68e-12 65.1
MS.gene037538.t1 MTR_7g063820 36.667 120 68 3 30 143 5 122 5.78e-15 74.7
MS.gene037538.t1 MTR_7g063820 29.762 252 152 7 31 267 243 484 1.31e-12 67.8
MS.gene037538.t1 MTR_0150s0030 35.849 106 59 3 30 132 3 102 1.12e-14 71.2
MS.gene037538.t1 MTR_7g058700 47.126 87 43 2 35 118 752 838 1.22e-14 73.9
MS.gene037538.t1 MTR_8g445910 36.792 106 58 3 30 132 3 102 1.93e-14 71.6
MS.gene037538.t1 MTR_3g065200 32.161 199 108 6 48 242 225 400 2.92e-14 72.4
MS.gene037538.t1 MTR_7g063880 27.731 238 152 6 35 267 246 468 3.36e-14 72.4
MS.gene037538.t1 MTR_7g063880 34.211 114 66 3 30 135 6 118 4.15e-12 66.2
MS.gene037538.t1 MTR_7g063840 27.801 241 157 6 31 267 246 473 4.30e-14 72.0
MS.gene037538.t1 MTR_1g115455 38.372 86 51 1 30 113 4 89 1.77e-13 68.6
MS.gene037538.t1 MTR_7g066290 37.209 129 78 1 1 126 233 361 1.78e-13 70.1
MS.gene037538.t1 MTR_8g044880 36.792 106 58 3 30 132 3 102 1.87e-13 68.9
MS.gene037538.t1 MTR_0150s0010 27.004 237 153 7 30 255 5 232 4.56e-13 67.4
MS.gene037538.t1 MTR_1g115440 27.917 240 147 8 30 255 6 233 1.19e-11 63.5
MS.gene037538.t1 MTR_8g445920 36.364 77 49 0 30 106 5 81 1.20e-11 60.5
MS.gene037538.t1 MTR_8g044890 36.364 77 49 0 30 106 5 81 1.20e-11 60.5
MS.gene037538.t1 MTR_1g115470 36.047 86 53 1 31 114 2 87 3.25e-11 63.5
MS.gene037538.t1 MTR_7g063830 34.545 110 63 4 38 140 13 120 4.63e-11 62.8
MS.gene037538.t1 MTR_7g063850 26.383 235 153 7 38 267 69 288 6.95e-11 61.6
MS.gene037538.t1 MTR_7g066490 29.101 189 103 6 29 208 10 176 8.70e-11 62.0
MS.gene037538.t1 MTR_1g014060 29.362 235 127 8 38 267 190 390 8.75e-11 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene037538.t1 AT5G01140 32.210 267 146 13 16 267 212 458 2.19e-19 87.4
MS.gene037538.t1 AT5G01120 29.779 272 159 9 1 257 231 485 4.02e-18 84.0
MS.gene037538.t1 AT5G01130 28.455 246 133 7 28 267 243 451 1.75e-17 82.0
MS.gene037538.t1 AT3G09140 27.984 243 154 9 30 254 4 243 2.98e-14 72.4
MS.gene037538.t1 AT3G09140 30.180 222 130 9 48 262 291 494 8.57e-14 71.2
MS.gene037538.t1 AT3G09140 28.846 208 130 8 30 222 4 208 4.19e-14 72.0
MS.gene037538.t1 AT3G09140 30.180 222 130 9 48 262 319 522 9.97e-14 71.2
MS.gene037538.t1 AT5G01150 29.565 230 132 8 48 267 276 485 1.10e-13 70.9
MS.gene037538.t1 AT5G43240 28.881 277 156 11 1 257 90 345 8.09e-13 68.2
MS.gene037538.t1 AT3G09120 28.696 230 149 6 35 255 6 229 1.08e-12 67.4
MS.gene037538.t1 AT5G43240 28.881 277 156 11 1 257 234 489 1.60e-12 67.4
MS.gene037538.t1 AT5G43240 28.807 243 132 11 36 254 10 235 3.72e-12 66.2
MS.gene037538.t1 AT5G43240 28.881 277 156 11 1 257 234 489 1.60e-12 67.4
MS.gene037538.t1 AT5G43240 28.807 243 132 11 36 254 10 235 3.72e-12 66.2
MS.gene037538.t1 AT5G43240 28.881 277 156 11 1 257 234 489 1.60e-12 67.4
MS.gene037538.t1 AT5G43240 28.807 243 132 11 36 254 10 235 3.72e-12 66.2
MS.gene037538.t1 AT5G37320 28.980 245 136 11 28 261 262 479 2.48e-11 63.9
MS.gene037538.t1 AT5G37320 32.110 109 60 3 31 128 5 110 3.16e-11 63.5

Find 70 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTGACAAGTTGGGAGATTT+TGG 0.140906 4.3:+82102468 None:intergenic
CCTGGCGCAGCACCACATTT+TGG 0.210743 4.3:-82102672 MS.gene037538:CDS
ACCATCTGAAAGCTTTAAAA+TGG 0.212595 4.3:+82102788 None:intergenic
CACAAGTGGCGGCACTAAAA+TGG 0.259934 4.3:-82102916 MS.gene037538:CDS
AAGTGGGCTGGGAAACCTTT+TGG 0.316630 4.3:-82102156 MS.gene037538:CDS
AGGCAGGTTGCCTTTGTACT+TGG 0.329588 4.3:+82102974 None:intergenic
GTAATAGTTTATTCTTGTTT+AGG 0.331118 4.3:+82102128 None:intergenic
AGCTTCCTTACCATGCCATT+AGG 0.332201 4.3:-82102813 MS.gene037538:CDS
TTCAAAATTGTATTTGTCAT+AGG 0.339563 4.3:+82103005 None:intergenic
GCTTCCTTACCATGCCATTA+GGG 0.353748 4.3:-82102812 MS.gene037538:CDS
CTCTAACGGCTGATGAAGAT+TGG 0.361391 4.3:+82102949 None:intergenic
TCAGATGGTAACTCGTTTGC+TGG 0.365227 4.3:-82102774 MS.gene037538:CDS
GTTGCGGGCGGTCGTAAATC+AGG 0.366748 4.3:+82102624 None:intergenic
CCGCCCGCAACATATTATTA+TGG 0.379537 4.3:-82102612 MS.gene037538:CDS
GGAATGTTGAGGGGCTGGTT+TGG 0.384748 4.3:+82102645 None:intergenic
CTTGTGGTGTAGAGCTTCCT+TGG 0.418297 4.3:-82102209 MS.gene037538:intron
AACTCGTTTGCTGGATGTAT+TGG 0.424872 4.3:-82102765 MS.gene037538:CDS
CCAAAATGTGGTGCTGCGCC+AGG 0.440390 4.3:+82102672 None:intergenic
GTACCATAATAATATGTTGC+GGG 0.440914 4.3:+82102609 None:intergenic
CTCCACTTCAAGATCATCAA+TGG 0.442551 4.3:+82102329 None:intergenic
CGCACTGTGTTACTCAATCC+TGG 0.461909 4.3:-82102690 MS.gene037538:CDS
GATAAGCATTGGTGAATTTG+AGG 0.462994 4.3:-82102304 MS.gene037538:intron
CGGCACTAAAATGGATGTTA+AGG 0.464791 4.3:-82102907 MS.gene037538:CDS
GTGCAGAGACTAGCATTGTA+TGG 0.467656 4.3:-82102426 MS.gene037538:CDS
CCGCCTTCCCTTGACAAGTT+GGG 0.467668 4.3:+82102459 None:intergenic
AGACTAGCATTGTATGGTAT+AGG 0.468200 4.3:-82102420 MS.gene037538:CDS
GTACAAGAGTGTGGAGAACT+TGG 0.471755 4.3:-82102736 MS.gene037538:CDS
GTTTACCTTGACAAGTGGGC+TGG 0.472396 4.3:-82102168 MS.gene037538:CDS
TCAAAGTTTACCTTGACAAG+TGG 0.475130 4.3:-82102173 MS.gene037538:CDS
TTTACCTTGACAAGTGGGCT+GGG 0.476046 4.3:-82102167 MS.gene037538:CDS
ACTTGTCAAGGTAAACTTTG+AGG 0.478186 4.3:+82102175 None:intergenic
AATCAGGAATGTTGAGGGGC+TGG 0.489501 4.3:+82102640 None:intergenic
AGCTTTAAAATGGACCCTAA+TGG 0.492701 4.3:+82102798 None:intergenic
CTTGTTTAGGCACACCCAAA+AGG 0.499787 4.3:+82102141 None:intergenic
ACCGCCTTCCCTTGACAAGT+TGG 0.507948 4.3:+82102458 None:intergenic
CAAAATCTCCCAACTTGTCA+AGG 0.521464 4.3:-82102467 MS.gene037538:CDS
TTGTCAAGGGAAGGCGGTGT+GGG 0.525762 4.3:-82102453 MS.gene037538:CDS
TACGTTTGAAGTAAAGATAG+AGG 0.528667 4.3:-82102553 MS.gene037538:CDS
TCCACTTCAAGATCATCAAT+GGG 0.538387 4.3:+82102330 None:intergenic
CTGATGAAGATTGGTTAGGC+AGG 0.539982 4.3:+82102958 None:intergenic
ACGGCTGATGAAGATTGGTT+AGG 0.542485 4.3:+82102954 None:intergenic
TCGTAAATCAGGAATGTTGA+GGG 0.544479 4.3:+82102635 None:intergenic
ATAATTCTTTCAAATATACA+AGG 0.564110 4.3:+82102358 None:intergenic
TCCCATTGATGATCTTGAAG+TGG 0.570161 4.3:-82102331 MS.gene037538:CDS
CTTGTCAAGGGAAGGCGGTG+TGG 0.575577 4.3:-82102454 MS.gene037538:CDS
AGAGTAAAACCTTGCACAAG+TGG 0.576824 4.3:-82102930 MS.gene037538:CDS
TGGACCCTAATGGCATGGTA+AGG 0.578120 4.3:+82102808 None:intergenic
GTTTCCCAGCCCACTTGTCA+AGG 0.584219 4.3:+82102163 None:intergenic
TTAGTGCCGCCACTTGTGCA+AGG 0.589354 4.3:+82102921 None:intergenic
CAAAGTTTACCTTGACAAGT+GGG 0.590983 4.3:-82102172 MS.gene037538:CDS
TGGCAATTTGTACAAGAGTG+TGG 0.591892 4.3:-82102745 MS.gene037538:CDS
TAAAATGGACCCTAATGGCA+TGG 0.595393 4.3:+82102803 None:intergenic
TTTGAAGTAAAGATAGAGGG+AGG 0.598835 4.3:-82102549 MS.gene037538:CDS
AAAATCTCCCAACTTGTCAA+GGG 0.599820 4.3:-82102466 MS.gene037538:CDS
TCTCCCAACTTGTCAAGGGA+AGG 0.600220 4.3:-82102462 MS.gene037538:CDS
GTCGTAAATCAGGAATGTTG+AGG 0.607776 4.3:+82102634 None:intergenic
GAAGTCAACAAAATCACCGT+TGG 0.609986 4.3:+82102842 None:intergenic
CTTCAAGATCATCAATGGGA+AGG 0.610347 4.3:+82102334 None:intergenic
CCCAACTTGTCAAGGGAAGG+CGG 0.621164 4.3:-82102459 MS.gene037538:CDS
AAAAGTAGCTCCAAGTACAA+AGG 0.632301 4.3:-82102984 MS.gene037538:CDS
ACGTTTGAAGTAAAGATAGA+GGG 0.638925 4.3:-82102552 MS.gene037538:CDS
TTGAGGTCAAGAATTCTCCA+AGG 0.639119 4.3:+82102192 None:intergenic
CATTGATGATCTTGAAGTGG+AGG 0.646684 4.3:-82102328 MS.gene037538:CDS
ATCATCTTTGTAGAAGCCAA+CGG 0.648266 4.3:-82102858 MS.gene037538:CDS
GTAAAACCTTGCACAAGTGG+CGG 0.651940 4.3:-82102927 MS.gene037538:CDS
GAAGTGGAGGTGATAAGCAT+TGG 0.663142 4.3:-82102315 MS.gene037538:CDS
TGTACCATAATAATATGTTG+CGG 0.667161 4.3:+82102608 None:intergenic
TGGTTTGGACAACCAAAATG+TGG 0.677088 4.3:+82102660 None:intergenic
CGTAAATCAGGAATGTTGAG+GGG 0.681609 4.3:+82102636 None:intergenic
CCATAATAATATGTTGCGGG+CGG 0.691477 4.3:+82102612 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAATTCTTTCAAATATACA+AGG + chr4.3:82102783-82102802 None:intergenic 15.0%
! TGTACCATAATAATATGTTG+CGG + chr4.3:82102533-82102552 None:intergenic 25.0%
!! CAAGAGTTTTGCATTAAAAA+TGG + chr4.3:82102638-82102657 None:intergenic 25.0%
GTACCATAATAATATGTTGC+GGG + chr4.3:82102532-82102551 None:intergenic 30.0%
! ACGTTTGAAGTAAAGATAGA+GGG - chr4.3:82102586-82102605 MS.gene037538:CDS 30.0%
! TACGTTTGAAGTAAAGATAG+AGG - chr4.3:82102585-82102604 MS.gene037538:CDS 30.0%
!! ACCATCTGAAAGCTTTAAAA+TGG + chr4.3:82102353-82102372 None:intergenic 30.0%
!!! TCCATTTTAAAGCTTTCAGA+TGG - chr4.3:82102349-82102368 MS.gene037538:CDS 30.0%
AAAAGTAGCTCCAAGTACAA+AGG - chr4.3:82102154-82102173 MS.gene037538:CDS 35.0%
AAAATCTCCCAACTTGTCAA+GGG - chr4.3:82102672-82102691 MS.gene037538:CDS 35.0%
ACTTGTCAAGGTAAACTTTG+AGG + chr4.3:82102966-82102985 None:intergenic 35.0%
AGACTAGCATTGTATGGTAT+AGG - chr4.3:82102718-82102737 MS.gene037538:CDS 35.0%
ATCATCTTTGTAGAAGCCAA+CGG - chr4.3:82102280-82102299 MS.gene037538:intron 35.0%
CAAAGTTTACCTTGACAAGT+GGG - chr4.3:82102966-82102985 MS.gene037538:CDS 35.0%
TCAAAGTTTACCTTGACAAG+TGG - chr4.3:82102965-82102984 MS.gene037538:CDS 35.0%
TCCACTTCAAGATCATCAAT+GGG + chr4.3:82102811-82102830 None:intergenic 35.0%
TCGTAAATCAGGAATGTTGA+GGG + chr4.3:82102506-82102525 None:intergenic 35.0%
! TGTGCAAGGTTTTACTCTAA+CGG + chr4.3:82102206-82102225 None:intergenic 35.0%
! TTTGAAGTAAAGATAGAGGG+AGG - chr4.3:82102589-82102608 MS.gene037538:CDS 35.0%
!! AGCTTTAAAATGGACCCTAA+TGG + chr4.3:82102343-82102362 None:intergenic 35.0%
!! GATAAGCATTGGTGAATTTG+AGG - chr4.3:82102834-82102853 MS.gene037538:CDS 35.0%
ACAAGCACAAGGAATCAAAG+AGG + chr4.3:82102914-82102933 None:intergenic 40.0%
AGAGTAAAACCTTGCACAAG+TGG - chr4.3:82102208-82102227 MS.gene037538:intron 40.0%
CAAAATCTCCCAACTTGTCA+AGG - chr4.3:82102671-82102690 MS.gene037538:CDS 40.0%
CCATAATAATATGTTGCGGG+CGG + chr4.3:82102529-82102548 None:intergenic 40.0%
CGGCACTAAAATGGATGTTA+AGG - chr4.3:82102231-82102250 MS.gene037538:intron 40.0%
CGTAAATCAGGAATGTTGAG+GGG + chr4.3:82102505-82102524 None:intergenic 40.0%
CTCCACTTCAAGATCATCAA+TGG + chr4.3:82102812-82102831 None:intergenic 40.0%
CTTCAAGATCATCAATGGGA+AGG + chr4.3:82102807-82102826 None:intergenic 40.0%
GAAGTCAACAAAATCACCGT+TGG + chr4.3:82102299-82102318 None:intergenic 40.0%
GTCGTAAATCAGGAATGTTG+AGG + chr4.3:82102507-82102526 None:intergenic 40.0%
TAAAATGGACCCTAATGGCA+TGG + chr4.3:82102338-82102357 None:intergenic 40.0%
TCTTTGATTCCTTGTGCTTG+TGG - chr4.3:82102913-82102932 MS.gene037538:CDS 40.0%
TGGCAATTTGTACAAGAGTG+TGG - chr4.3:82102393-82102412 MS.gene037538:CDS 40.0%
TTGAGGTCAAGAATTCTCCA+AGG + chr4.3:82102949-82102968 None:intergenic 40.0%
! AACTCGTTTGCTGGATGTAT+TGG - chr4.3:82102373-82102392 MS.gene037538:CDS 40.0%
! CATTGATGATCTTGAAGTGG+AGG - chr4.3:82102810-82102829 MS.gene037538:CDS 40.0%
! GCATTGGTGAATTTGAGGTA+TGG - chr4.3:82102839-82102858 MS.gene037538:CDS 40.0%
! TCCCATTGATGATCTTGAAG+TGG - chr4.3:82102807-82102826 MS.gene037538:CDS 40.0%
! TGGTTTGGACAACCAAAATG+TGG + chr4.3:82102481-82102500 None:intergenic 40.0%
! TTGGTGAATTTGAGGTATGG+TGG - chr4.3:82102842-82102861 MS.gene037538:CDS 40.0%
!! CTTGACAAGTTGGGAGATTT+TGG + chr4.3:82102673-82102692 None:intergenic 40.0%
AGCTTCCTTACCATGCCATT+AGG - chr4.3:82102325-82102344 MS.gene037538:CDS 45.0%
CCGCCCGCAACATATTATTA+TGG - chr4.3:82102526-82102545 MS.gene037538:CDS 45.0%
CTCTAACGGCTGATGAAGAT+TGG + chr4.3:82102192-82102211 None:intergenic 45.0%
CTTGTTTAGGCACACCCAAA+AGG + chr4.3:82103000-82103019 None:intergenic 45.0%
GAAGTGGAGGTGATAAGCAT+TGG - chr4.3:82102823-82102842 MS.gene037538:CDS 45.0%
GCTTCCTTACCATGCCATTA+GGG - chr4.3:82102326-82102345 MS.gene037538:CDS 45.0%
GTAAAACCTTGCACAAGTGG+CGG - chr4.3:82102211-82102230 MS.gene037538:intron 45.0%
GTGCAGAGACTAGCATTGTA+TGG - chr4.3:82102712-82102731 MS.gene037538:CDS 45.0%
TCAGATGGTAACTCGTTTGC+TGG - chr4.3:82102364-82102383 MS.gene037538:CDS 45.0%
! ACGGCTGATGAAGATTGGTT+AGG + chr4.3:82102187-82102206 None:intergenic 45.0%
! CTGATGAAGATTGGTTAGGC+AGG + chr4.3:82102183-82102202 None:intergenic 45.0%
! GTACAAGAGTGTGGAGAACT+TGG - chr4.3:82102402-82102421 MS.gene037538:CDS 45.0%
! TTTACCTTGACAAGTGGGCT+GGG - chr4.3:82102971-82102990 MS.gene037538:CDS 45.0%
AATCAGGAATGTTGAGGGGC+TGG + chr4.3:82102501-82102520 None:intergenic 50.0%
AGCTCTACACCACAAGCACA+AGG + chr4.3:82102925-82102944 None:intergenic 50.0%
AGGCAGGTTGCCTTTGTACT+TGG + chr4.3:82102167-82102186 None:intergenic 50.0%
CACAAGTGGCGGCACTAAAA+TGG - chr4.3:82102222-82102241 MS.gene037538:intron 50.0%
CTTGTGGTGTAGAGCTTCCT+TGG - chr4.3:82102929-82102948 MS.gene037538:CDS 50.0%
GTTTACCTTGACAAGTGGGC+TGG - chr4.3:82102970-82102989 MS.gene037538:CDS 50.0%
TCTCCCAACTTGTCAAGGGA+AGG - chr4.3:82102676-82102695 MS.gene037538:CDS 50.0%
TGGACCCTAATGGCATGGTA+AGG + chr4.3:82102333-82102352 None:intergenic 50.0%
! CGCACTGTGTTACTCAATCC+TGG - chr4.3:82102448-82102467 MS.gene037538:CDS 50.0%
!!! AAGTGGGCTGGGAAACCTTT+TGG - chr4.3:82102982-82103001 MS.gene037538:CDS 50.0%
!!! AGTGGGCTGGGAAACCTTTT+GGG - chr4.3:82102983-82103002 MS.gene037538:CDS 50.0%
ACCGCCTTCCCTTGACAAGT+TGG + chr4.3:82102683-82102702 None:intergenic 55.0%
CCCAACTTGTCAAGGGAAGG+CGG - chr4.3:82102679-82102698 MS.gene037538:CDS 55.0%
CCGCCTTCCCTTGACAAGTT+GGG + chr4.3:82102682-82102701 None:intergenic 55.0%
GTTTCCCAGCCCACTTGTCA+AGG + chr4.3:82102978-82102997 None:intergenic 55.0%
TTAGTGCCGCCACTTGTGCA+AGG + chr4.3:82102220-82102239 None:intergenic 55.0%
TTGTCAAGGGAAGGCGGTGT+GGG - chr4.3:82102685-82102704 MS.gene037538:CDS 55.0%
!! GGAATGTTGAGGGGCTGGTT+TGG + chr4.3:82102496-82102515 None:intergenic 55.0%
CTTGTCAAGGGAAGGCGGTG+TGG - chr4.3:82102684-82102703 MS.gene037538:CDS 60.0%
GTTGCGGGCGGTCGTAAATC+AGG + chr4.3:82102517-82102536 None:intergenic 60.0%
! CCTGGCGCAGCACCACATTT+TGG - chr4.3:82102466-82102485 MS.gene037538:CDS 60.0%
!! CCAAAATGTGGTGCTGCGCC+AGG + chr4.3:82102469-82102488 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 82102136 82103024 82102136 ID=MS.gene037538
chr4.3 mRNA 82102136 82103024 82102136 ID=MS.gene037538.t1;Parent=MS.gene037538
chr4.3 exon 82102305 82103024 82102305 ID=MS.gene037538.t1.exon1;Parent=MS.gene037538.t1
chr4.3 CDS 82102305 82103024 82102305 ID=cds.MS.gene037538.t1;Parent=MS.gene037538.t1
chr4.3 exon 82102136 82102219 82102136 ID=MS.gene037538.t1.exon2;Parent=MS.gene037538.t1
chr4.3 CDS 82102136 82102219 82102136 ID=cds.MS.gene037538.t1;Parent=MS.gene037538.t1
Gene Sequence

>MS.gene037538

ATGACAAATACAATTTTGAAAAGTAGCTCCAAGTACAAAGGCAACCTGCCTAACCAATCTTCATCAGCCGTTAGAGTAAAACCTTGCACAAGTGGCGGCACTAAAATGGATGTTAAGGTAGTTCGAAGTATATCTCAGAAAAAGATCATCTTTGTAGAAGCCAACGGTGATTTTGTTGACTTCATTTTTAGCTTCCTTACCATGCCATTAGGGTCCATTTTAAAGCTTTCAGATGGTAACTCGTTTGCTGGATGTATTGGCAATTTGTACAAGAGTGTGGAGAACTTGGACTCTTCATTGTGTACTGACTCACGCACTGTGTTACTCAATCCTGGCGCAGCACCACATTTTGGTTGTCCAAACCAGCCCCTCAACATTCCTGATTTACGACCGCCCGCAACATATTATTATGGTACATGTGCATCACATAAATCTATTAATAGTACACGTACGTTTGAAGTAAAGATAGAGGGAGGAGTGATTTCTAAGACAAAAGAATCCATTTTTAATGCAAAACTCTTGACTGCACTTGATCCAAAATCTCCCAACTTGTCAAGGGAAGGCGGTGTGGGATTTGTGCAGAGACTAGCATTGTATGGTATAGGAGATGATCTAAAACTAAAATCACTTTCTGCTAATTCTTTCCTTGTATATTTGAAAGAATTATGCCTTCCCATTGATGATCTTGAAGTGGAGGTGATAAGCATTGGTGAATTTGAGGTATGGTGGTTATTATTTTATTACTGAATTTATAATTTTTTCTATTTTATTTCAACCTCTTTGATTCCTTGTGCTTGTGGTGTAGAGCTTCCTTGGAGAATTCTTGACCTCAAAGTTTACCTTGACAAGTGGGCTGGGAAACCTTTTGGGTGTGCCTAAACAAGAATAA
Protein sequence

>MS.gene037538.t1

MTNTILKSSSKYKGNLPNQSSSAVRVKPCTSGGTKMDVKVVRSISQKKIIFVEANGDFVDFIFSFLTMPLGSILKLSDGNSFAGCIGNLYKSVENLDSSLCTDSRTVLLNPGAAPHFGCPNQPLNIPDLRPPATYYYGTCASHKSINSTRTFEVKIEGGVISKTKESIFNAKLLTALDPKSPNLSREGGVGFVQRLALYGIGDDLKLKSLSANSFLVYLKELCLPIDDLEVEVISIGEFESFLGEFLTSKFTLTSGLGNLLGVPKQE