AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1087810


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1087810 MtrunA17_Chr4g0071331 90.476 84 7 1 1 84 296 378 1.24e-48 157
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 10 sgRNAs with CRISPR-Local

Find 17 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCAATTTCTCCTCCCTTGA+AGG 0.247560 7_3:+594148 None:intergenic
CGGAGTATGTGAATCTTATA+TGG 0.339164 7_3:-594069 Msa1087810:CDS
TGCTTTCCACATTATTAGAA+TGG 0.377837 7_3:-594186 Msa1087810:CDS
ATGAAGTAGATCCACCTTCA+AGG 0.498181 7_3:-594162 Msa1087810:CDS
TTCATTCCATTCTAATAATG+TGG 0.499048 7_3:+594180 None:intergenic
AATTTCTCCTCCCTTGAAGG+TGG 0.505594 7_3:+594151 None:intergenic
CATATAAGATTCACATACTC+CGG 0.541628 7_3:+594070 None:intergenic
TACTCCGGATGATCAACAAA+AGG 0.542530 7_3:+594085 None:intergenic
TGAAGTAGATCCACCTTCAA+GGG 0.586411 7_3:-594161 Msa1087810:CDS
AGTAGATCCACCTTCAAGGG+AGG 0.669378 7_3:-594158 Msa1087810:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GTAAATTTTATCAGCATAAT+CGG - chr7_3:594043-594062 Msa1087810:CDS 20.0%
!!! TAATTTTTTTTCTTCTTCGT+TGG + chr7_3:594166-594185 None:intergenic 20.0%
!! AACGAAGAAGAAAAAAAATT+AGG - chr7_3:594165-594184 Msa1087810:CDS 20.0%
! TTCATTCCATTCTAATAATG+TGG + chr7_3:593977-593996 None:intergenic 25.0%
! AAGAAAAAAAATTAGGACGT+GGG - chr7_3:594172-594191 Msa1087810:CDS 25.0%
TGCTTTCCACATTATTAGAA+TGG - chr7_3:593968-593987 Msa1087810:CDS 30.0%
CATATAAGATTCACATACTC+CGG + chr7_3:594087-594106 None:intergenic 30.0%
GAAGAAAAAAAATTAGGACG+TGG - chr7_3:594171-594190 Msa1087810:CDS 30.0%
AGAAAAAAAATTAGGACGTG+GGG - chr7_3:594173-594192 Msa1087810:CDS 30.0%
! GAATCCTTTTGTTGATCATC+CGG - chr7_3:594065-594084 Msa1087810:CDS 35.0%
CGGAGTATGTGAATCTTATA+TGG - chr7_3:594085-594104 Msa1087810:CDS 35.0%
ATGAAGTAGATCCACCTTCA+AGG - chr7_3:593992-594011 Msa1087810:CDS 40.0%
TGAAGTAGATCCACCTTCAA+GGG - chr7_3:593993-594012 Msa1087810:CDS 40.0%
TACTCCGGATGATCAACAAA+AGG + chr7_3:594072-594091 None:intergenic 40.0%
AATTTCTCCTCCCTTGAAGG+TGG + chr7_3:594006-594025 None:intergenic 45.0%
CTCAATTTCTCCTCCCTTGA+AGG + chr7_3:594009-594028 None:intergenic 45.0%
AGTAGATCCACCTTCAAGGG+AGG - chr7_3:593996-594015 Msa1087810:CDS 50.0%
Chromosome Type Strat End Strand Name
chr7_3 gene 593961 594215 593961 ID=Msa1087810;Name=Msa1087810
chr7_3 mRNA 593961 594215 593961 ID=Msa1087810-mRNA-1;Parent=Msa1087810;Name=Msa1087810-mRNA-1;_AED=0.44;_eAED=0.45;_QI=0|-1|0|1|-1|1|1|0|84
chr7_3 exon 593961 594215 593961 ID=Msa1087810-mRNA-1:exon:349;Parent=Msa1087810-mRNA-1
chr7_3 CDS 593961 594215 593961 ID=Msa1087810-mRNA-1:cds;Parent=Msa1087810-mRNA-1
Gene Sequence

>Msa1087810

ATGGGACTGCTTTCCACATTATTAGAATGGAATGAAGTAGATCCACCTTCAAGGGAGGAGAAATTGAGAAACGAGAGAATATGTAAATTTTATCAGCATAATCGGAATCCTTTTGTTGATCATCCGGAGTATGTGAATCTTATATGGAAGCGACTTGTTTCAATGCGTGCTCATAATAATAACGATGATGATAAGAATGTCTCCAACGAAGAAGAAAAAAAATTAGGACGTGGGGCTCAATTGCTTCACTTATGA

Protein sequence

>Msa1087810

MGLLSTLLEWNEVDPPSREEKLRNERICKFYQHNRNPFVDHPEYVNLIWKRLVSMRAHNNNDDDKNVSNEEEKKLGRGAQLLHL*