AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa0104770


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104770 MtrunA17_Chr1g0159601 93.689 206 12 1 1 206 46 250 4.65e-142 397
Msa0104770 MtrunA17_Chr1g0159611 79.412 136 28 0 1 136 46 181 4.85e-75 236
Msa0104770 MtrunA17_Chr2g0277731 43.439 221 109 6 6 216 6 220 7.67e-50 171
Msa0104770 MtrunA17_Chr8g0380681 45.370 216 99 5 8 205 5 219 1.04e-49 173
Msa0104770 MtrunA17_Chr8g0380681 38.000 200 111 6 18 205 691 889 1.71e-35 132
Msa0104770 MtrunA17_Chr4g0039131 45.000 120 63 2 86 203 1 119 7.28e-29 106
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa0104770 AT1G11050.1 42.246 187 101 4 25 205 26 211 3.74e-36 134

Find 34 sgRNAs with CRISPR-Local

Find 41 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAATCTCTTCTTAGTCTCTT+TGG 0.194083 1_3:-12927376 Msa0104770:CDS
GTTGGTATTGTGAATCATTT+TGG 0.240330 1_3:-12926977 Msa0104770:CDS
GAAAGAGTGAAGTTTCTTTC+AGG 0.244676 1_3:+12927335 None:intergenic
CAGAAGGAGTAGCTATGATA+AGG 0.380662 1_3:+12927497 None:intergenic
TTGTAAAGGTGATTTGAAAT+CGG 0.388227 1_3:-12927110 Msa0104770:CDS
CAACAATGTTGTGTGTCAAT+TGG 0.393976 1_3:+12927400 None:intergenic
TGTGTCAATTGGGTCTCTGC+AGG 0.430262 1_3:+12927411 None:intergenic
CTTCTTAGTCTCTTTGGCAT+AGG 0.437265 1_3:-12927370 Msa0104770:CDS
CTTTACAAGAAGTGTCTAAA+GGG 0.447705 1_3:+12927125 None:intergenic
TAAGGGTGAAGAGGAGGAGA+AGG 0.460180 1_3:+12927515 None:intergenic
CCTTTAGACACTTCTTGTAA+AGG 0.468879 1_3:-12927124 Msa0104770:CDS
CTAGTTGTATACTTGGTAGT+TGG 0.472192 1_3:-12926934 Msa0104770:CDS
AACAATGTTGTGTGTCAATT+GGG 0.477153 1_3:+12927401 None:intergenic
TGCAGCACGTGCACTAATGC+TGG 0.478276 1_3:-12927079 Msa0104770:CDS
GTATCACAAACATCAAAGAT+TGG 0.490764 1_3:-12927171 Msa0104770:CDS
TATTGAGAGTAGATAGAAGC+GGG 0.491909 1_3:+12926908 None:intergenic
CTATGATAAGGGTGAAGAGG+AGG 0.492847 1_3:+12927509 None:intergenic
CCTTTACAAGAAGTGTCTAA+AGG 0.505136 1_3:+12927124 None:intergenic
TAGCTATGATAAGGGTGAAG+AGG 0.511716 1_3:+12927506 None:intergenic
GCTACAGCTAGTTGTATACT+TGG 0.511927 1_3:-12926941 Msa0104770:CDS
AGAAGGAGTAGCTATGATAA+GGG 0.514017 1_3:+12927498 None:intergenic
TTCTGCAGTATCATGTCCTA+TGG 0.534711 1_3:-12927473 Msa0104770:CDS
AAGAAGGGACTAACGAAGGT+TGG 0.536160 1_3:+12927242 None:intergenic
TGACAAATTGAGTTGAGTTG+TGG 0.541730 1_3:+12927215 None:intergenic
TCAACATAGCTGAGATCCAT+AGG 0.544235 1_3:+12927457 None:intergenic
GGTTGATGTGTCCCATGAAA+AGG 0.549361 1_3:+12927432 None:intergenic
ACAAACAACTCAAGCTGCGC+AGG 0.551790 1_3:-12927193 Msa0104770:CDS
GTTGAGTTGTGGAAACAAGA+AGG 0.556459 1_3:+12927226 None:intergenic
AGTATACAACTAGCTGTAGC+AGG 0.563837 1_3:+12926944 None:intergenic
CTAGCTGTAGCAGGATCTGT+AGG 0.582604 1_3:+12926953 None:intergenic
CATGATACTGCAGAAGCAGA+AGG 0.601540 1_3:+12927481 None:intergenic
TTGAGTTGTGGAAACAAGAA+GGG 0.613460 1_3:+12927227 None:intergenic
ATATTGAGAGTAGATAGAAG+CGG 0.644838 1_3:+12926907 None:intergenic
AAACAAGAAGGGACTAACGA+AGG 0.740560 1_3:+12927238 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTTTAAAGTGTTTTTATTA+GGG + chr1_3:12927119-12927138 None:intergenic 10.0%
!!! GATTTTAAAGTGTTTTTATT+AGG + chr1_3:12927120-12927139 None:intergenic 15.0%
!! TTGTAAAGGTGATTTGAAAT+CGG - chr1_3:12927314-12927333 Msa0104770:CDS 25.0%
!!! TTTTCAGTTTTATATGCTGT+TGG - chr1_3:12927429-12927448 Msa0104770:CDS 25.0%
AACAATGTTGTGTGTCAATT+GGG + chr1_3:12927026-12927045 None:intergenic 30.0%
GTATCACAAACATCAAAGAT+TGG - chr1_3:12927253-12927272 Msa0104770:CDS 30.0%
AAAGATTGGAAGCAAAAAGT+TGG - chr1_3:12927267-12927286 Msa0104770:CDS 30.0%
!! CTTTACAAGAAGTGTCTAAA+GGG + chr1_3:12927302-12927321 None:intergenic 30.0%
!!! AAGCACTTAGTTGCATTTTT+TGG + chr1_3:12927405-12927424 None:intergenic 30.0%
! GTTGGTATTGTGAATCATTT+TGG - chr1_3:12927447-12927466 Msa0104770:CDS 30.0%
ATATTGAGAGTAGATAGAAG+CGG + chr1_3:12927520-12927539 None:intergenic 30.0%
AGAAGGAGTAGCTATGATAA+GGG + chr1_3:12926929-12926948 None:intergenic 35.0%
! CTATGTTGAGACCTTTTCAT+GGG - chr1_3:12926981-12927000 Msa0104770:CDS 35.0%
CAACAATGTTGTGTGTCAAT+TGG + chr1_3:12927027-12927046 None:intergenic 35.0%
CAATCTCTTCTTAGTCTCTT+TGG - chr1_3:12927048-12927067 Msa0104770:CDS 35.0%
GAAAGAGTGAAGTTTCTTTC+AGG + chr1_3:12927092-12927111 None:intergenic 35.0%
! TTGAGTTGTGGAAACAAGAA+GGG + chr1_3:12927200-12927219 None:intergenic 35.0%
!! TGACAAATTGAGTTGAGTTG+TGG + chr1_3:12927212-12927231 None:intergenic 35.0%
!! CCTTTACAAGAAGTGTCTAA+AGG + chr1_3:12927303-12927322 None:intergenic 35.0%
CCTTTAGACACTTCTTGTAA+AGG - chr1_3:12927300-12927319 Msa0104770:CDS 35.0%
! CTAGTTGTATACTTGGTAGT+TGG - chr1_3:12927490-12927509 Msa0104770:CDS 35.0%
TATTGAGAGTAGATAGAAGC+GGG + chr1_3:12927519-12927538 None:intergenic 35.0%
TAGCTATGATAAGGGTGAAG+AGG + chr1_3:12926921-12926940 None:intergenic 40.0%
CAGAAGGAGTAGCTATGATA+AGG + chr1_3:12926930-12926949 None:intergenic 40.0%
TTCTGCAGTATCATGTCCTA+TGG - chr1_3:12926951-12926970 Msa0104770:CDS 40.0%
TCAACATAGCTGAGATCCAT+AGG + chr1_3:12926970-12926989 None:intergenic 40.0%
! GCTATGTTGAGACCTTTTCA+TGG - chr1_3:12926980-12926999 Msa0104770:CDS 40.0%
CTTCTTAGTCTCTTTGGCAT+AGG - chr1_3:12927054-12927073 Msa0104770:CDS 40.0%
AAACAAGAAGGGACTAACGA+AGG + chr1_3:12927189-12927208 None:intergenic 40.0%
! GTTGAGTTGTGGAAACAAGA+AGG + chr1_3:12927201-12927220 None:intergenic 40.0%
AGTATACAACTAGCTGTAGC+AGG + chr1_3:12927483-12927502 None:intergenic 40.0%
GCTACAGCTAGTTGTATACT+TGG - chr1_3:12927483-12927502 Msa0104770:CDS 40.0%
CTATGATAAGGGTGAAGAGG+AGG + chr1_3:12926918-12926937 None:intergenic 45.0%
CATGATACTGCAGAAGCAGA+AGG + chr1_3:12926946-12926965 None:intergenic 45.0%
! GGTTGATGTGTCCCATGAAA+AGG + chr1_3:12926995-12927014 None:intergenic 45.0%
AAGAAGGGACTAACGAAGGT+TGG + chr1_3:12927185-12927204 None:intergenic 45.0%
TAAGGGTGAAGAGGAGGAGA+AGG + chr1_3:12926912-12926931 None:intergenic 50.0%
TGTGTCAATTGGGTCTCTGC+AGG + chr1_3:12927016-12927035 None:intergenic 50.0%
ACAAACAACTCAAGCTGCGC+AGG - chr1_3:12927231-12927250 Msa0104770:CDS 50.0%
CTAGCTGTAGCAGGATCTGT+AGG + chr1_3:12927474-12927493 None:intergenic 50.0%
TGCAGCACGTGCACTAATGC+TGG - chr1_3:12927345-12927364 Msa0104770:CDS 55.0%
Chromosome Type Strat End Strand Name
chr1_3 gene 12926889 12927557 12926889 ID=Msa0104770;Name=Msa0104770
chr1_3 mRNA 12926889 12927557 12926889 ID=Msa0104770-mRNA-1;Parent=Msa0104770;Name=Msa0104770-mRNA-1;_AED=0.38;_eAED=0.38;_QI=0|-1|0|1|-1|1|1|0|222
chr1_3 exon 12926889 12927557 12926889 ID=Msa0104770-mRNA-1:exon:5072;Parent=Msa0104770-mRNA-1
chr1_3 CDS 12926889 12927557 12926889 ID=Msa0104770-mRNA-1:cds;Parent=Msa0104770-mRNA-1
Gene Sequence

>Msa0104770

ATGGATAACAAAGCAATGTTCCTTCTCCTCCTCTTCACCCTTATCATAGCTACTCCTTCTGCTTCTGCAGTATCATGTCCTATGGATCTCAGCTATGTTGAGACCTTTTCATGGGACACATCAACCTGCAGAGACCCAATTGACACACAACATTGTTGTCAATCTCTTCTTAGTCTCTTTGGCATAGGACTTGCAAAACACCTGAAAGAAACTTCACTCTTTCAACTCCCTAATAAAAACACTTTAAAATCTTGCTTACAAGATTTCAAACTCAAACTTTCATGCTTAAAGATCCAACCTTCGTTAGTCCCTTCTTGTTTCCACAACTCAACTCAATTTGTCACAAACAACTCAAGCTGCGCAGGTATCACAAACATCAAAGATTGGAAGCAAAAAGTTGGTAGAATTAGCCCTTTAGACACTTCTTGTAAAGGTGATTTGAAATCGGACACTAGTTGCAGCACGTGCACTAATGCTGGTTTCAAAGTCACTTCTCAGCTTACAAGTATTGATCCAAAAAATGCAACTAAGTGCTTTTTCTTTTCAGTTTTATATGCTGTTGGTATTGTGAATCATTTTGGTCCTACAGATCCTGCTACAGCTAGTTGTATACTTGGTAGTTGGTATCCCGCTTCTATCTACTCTCAATATAACAAAACAACATACTAG

Protein sequence

>Msa0104770

MDNKAMFLLLLFTLIIATPSASAVSCPMDLSYVETFSWDTSTCRDPIDTQHCCQSLLSLFGIGLAKHLKETSLFQLPNKNTLKSCLQDFKLKLSCLKIQPSLVPSCFHNSTQFVTNNSSCAGITNIKDWKQKVGRISPLDTSCKGDLKSDTSCSTCTNAGFKVTSQLTSIDPKNATKCFFFSVLYAVGIVNHFGPTDPATASCILGSWYPASIYSQYNKTTY*