AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280011240.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011240.01.T01 MTR_2g100500 92.523 107 8 0 3 109 420 526 1.52e-66 211
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280011240.01.T01 AT3G21300 79.245 106 22 0 3 108 414 519 7.29e-50 167

Find 33 sgRNAs with CRISPR-Local

Find 58 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AACAGTGTAAAGCATGTTTA+TGG 0.151368 2:-81373997 MsG0280011240.01.T01:CDS
TCAAGTTCCTCTTAAATCTT+AGG 0.161824 2:-81373360 MsG0280011240.01.T01:CDS
AATAAAATTGATGGAAATTT+CGG 0.162241 2:-81373883 MsG0280011240.01.T01:CDS
AAACCTGACATTGTTATTTC+AGG 0.189166 2:-81373847 MsG0280011240.01.T01:intron
TGCTTTACACTGTTCAAAAT+TGG 0.202725 2:+81374006 None:intergenic
CGACTTAATGCCAAGCTAAA+TGG 0.256646 2:-81373940 MsG0280011240.01.T01:CDS
ATAAAATTGATGGAAATTTC+GGG 0.260144 2:-81373882 MsG0280011240.01.T01:CDS
ATCAGCTTCATGTGCATCCC+TGG 0.321719 2:+81373382 None:intergenic
GAAATAACAATGTCAGGTTT+AGG 0.350429 2:+81373850 None:intergenic
CATGTGCATCCCTGGGCGGT+TGG 0.403322 2:+81373390 None:intergenic
GGGGATCTTAATAAAATTGA+TGG 0.405003 2:-81373892 MsG0280011240.01.T01:CDS
ATGTGCATCCCTGGGCGGTT+GGG 0.427113 2:+81373391 None:intergenic
TCAACTATCTGAGTTGTTGA+AGG 0.434192 2:-81373215 MsG0280011240.01.T01:CDS
ATTTAGCTTGGCATTAAGTC+GGG 0.446515 2:+81373942 None:intergenic
CAAGTTCCTCTTAAATCTTA+GGG 0.452080 2:-81373359 MsG0280011240.01.T01:CDS
ACAAAATGCAACATTTATTG+AGG 0.464324 2:-81373914 MsG0280011240.01.T01:CDS
CATTTAGCTTGGCATTAAGT+CGG 0.491991 2:+81373941 None:intergenic
CAAAATGCAACATTTATTGA+GGG 0.494797 2:-81373913 MsG0280011240.01.T01:CDS
GAACCTGAAATAACAATGTC+AGG 0.501451 2:+81373844 None:intergenic
AGGTCACGTGCACATGTAGC+AGG 0.511360 2:+81373307 None:intergenic
CGGGCATCTGCGATGGCTTG+AGG 0.525665 2:+81373961 None:intergenic
AATACAGATCCCAACCGCCC+AGG 0.534815 2:-81373400 MsG0280011240.01.T01:intron
ATACAGATCCCAACCGCCCA+GGG 0.549026 2:-81373399 MsG0280011240.01.T01:intron
GAGGCGCCCTAAGATTTAAG+AGG 0.561291 2:+81373353 None:intergenic
ATTAAGTCGGGCATCTGCGA+TGG 0.565964 2:+81373954 None:intergenic
TCAGCTTCATGTGCATCCCT+GGG 0.572316 2:+81373383 None:intergenic
GACCTTGATTATCTCTGTCA+TGG 0.592498 2:-81373289 MsG0280011240.01.T01:CDS
AAATGCAACATTTATTGAGG+GGG 0.600230 2:-81373911 MsG0280011240.01.T01:CDS
TGATTATCTCTGTCATGGTG+TGG 0.603583 2:-81373284 MsG0280011240.01.T01:CDS
AAAATGCAACATTTATTGAG+GGG 0.623055 2:-81373912 MsG0280011240.01.T01:CDS
CACCATGACAGAGATAATCA+AGG 0.625231 2:+81373287 None:intergenic
CATGAAACATACACTATGCG+AGG 0.646643 2:+81373334 None:intergenic
GCTTCATGTGCATCCCTGGG+CGG 0.687592 2:+81373386 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AATATATAAATAGAATAAGT+AGG + Chr2:81373416-81373435 None:intergenic 10.0%
!! AATAAAATTGATGGAAATTT+CGG - Chr2:81373330-81373349 MsG0280011240.01.T01:CDS 15.0%
!! TAATTAAAATAGAGCAAATA+TGG + Chr2:81373719-81373738 None:intergenic 15.0%
!! AAATAGAATAAGTAGGAATA+TGG + Chr2:81373409-81373428 None:intergenic 20.0%
!! ATAAAATTGATGGAAATTTC+GGG - Chr2:81373331-81373350 MsG0280011240.01.T01:CDS 20.0%
!! TTATTTATGTCTTTAATGGA+GGG - Chr2:81373618-81373637 MsG0280011240.01.T01:intron 20.0%
!! TTCGTTATTTATGTCTTTAA+TGG - Chr2:81373614-81373633 MsG0280011240.01.T01:intron 20.0%
! AAAATGCAACATTTATTGAG+GGG - Chr2:81373301-81373320 MsG0280011240.01.T01:CDS 25.0%
! ACAAAATGCAACATTTATTG+AGG - Chr2:81373299-81373318 MsG0280011240.01.T01:CDS 25.0%
! ACATGTTTAATTGTGTACTT+CGG + Chr2:81373776-81373795 None:intergenic 25.0%
! CAAAATGCAACATTTATTGA+GGG - Chr2:81373300-81373319 MsG0280011240.01.T01:CDS 25.0%
! CAATTAAACATGTTGTCATT+TGG - Chr2:81373783-81373802 MsG0280011240.01.T01:intron 25.0%
! CAGCTAACATTCAAATAAAT+GGG + Chr2:81373548-81373567 None:intergenic 25.0%
! GTTATTTATGTCTTTAATGG+AGG - Chr2:81373617-81373636 MsG0280011240.01.T01:intron 25.0%
! TAACATTCAAATAAATGGGT+TGG + Chr2:81373544-81373563 None:intergenic 25.0%
! TCAGCTAACATTCAAATAAA+TGG + Chr2:81373549-81373568 None:intergenic 25.0%
!! AACATTCAAATAAATGGGTT+GGG + Chr2:81373543-81373562 None:intergenic 25.0%
!! CAAATATGGTTTTCTTTACA+TGG + Chr2:81373705-81373724 None:intergenic 25.0%
AAATGCAACATTTATTGAGG+GGG - Chr2:81373302-81373321 MsG0280011240.01.T01:CDS 30.0%
AACAGTGTAAAGCATGTTTA+TGG - Chr2:81373216-81373235 MsG0280011240.01.T01:CDS 30.0%
AAGTAGGAATATGGAAATGT+AGG + Chr2:81373400-81373419 None:intergenic 30.0%
CAAGTTCCTCTTAAATCTTA+GGG - Chr2:81373854-81373873 MsG0280011240.01.T01:CDS 30.0%
CAATGGCAATTTCAACAATT+AGG + Chr2:81373499-81373518 None:intergenic 30.0%
GAAATAACAATGTCAGGTTT+AGG + Chr2:81373366-81373385 None:intergenic 30.0%
GGGGATCTTAATAAAATTGA+TGG - Chr2:81373321-81373340 MsG0280011240.01.T01:CDS 30.0%
TCAAGTTCCTCTTAAATCTT+AGG - Chr2:81373853-81373872 MsG0280011240.01.T01:CDS 30.0%
TGCTTTACACTGTTCAAAAT+TGG + Chr2:81373210-81373229 None:intergenic 30.0%
!! AAACCTGACATTGTTATTTC+AGG - Chr2:81373366-81373385 MsG0280011240.01.T01:CDS 30.0%
!! ATTTTGTATGCCATTTAGCT+TGG + Chr2:81373286-81373305 None:intergenic 30.0%
GAACCTGAAATAACAATGTC+AGG + Chr2:81373372-81373391 None:intergenic 35.0%
TCAACTATCTGAGTTGTTGA+AGG - Chr2:81373998-81374017 MsG0280011240.01.T01:CDS 35.0%
! TTTAATGGAGGGAGATTTTC+TGG - Chr2:81373629-81373648 MsG0280011240.01.T01:intron 35.0%
!! AGTTGTTGAAGGTTTTGTGT+AGG - Chr2:81374009-81374028 MsG0280011240.01.T01:CDS 35.0%
!! ATTCAAATAAATGGGTTGGG+TGG + Chr2:81373540-81373559 None:intergenic 35.0%
!! ATTTAGCTTGGCATTAAGTC+GGG + Chr2:81373274-81373293 None:intergenic 35.0%
!! CATTTAGCTTGGCATTAAGT+CGG + Chr2:81373275-81373294 None:intergenic 35.0%
!! TTCAAATAAATGGGTTGGGT+GGG + Chr2:81373539-81373558 None:intergenic 35.0%
CACCATGACAGAGATAATCA+AGG + Chr2:81373929-81373948 None:intergenic 40.0%
CATGAAACATACACTATGCG+AGG + Chr2:81373882-81373901 None:intergenic 40.0%
CGACTTAATGCCAAGCTAAA+TGG - Chr2:81373273-81373292 MsG0280011240.01.T01:CDS 40.0%
GACCTTGATTATCTCTGTCA+TGG - Chr2:81373924-81373943 MsG0280011240.01.T01:CDS 40.0%
GGGAAAGAAGTGAACAACAA+TGG + Chr2:81373516-81373535 None:intergenic 40.0%
TGATTATCTCTGTCATGGTG+TGG - Chr2:81373929-81373948 MsG0280011240.01.T01:CDS 40.0%
!! TCAAATAAATGGGTTGGGTG+GGG + Chr2:81373538-81373557 None:intergenic 40.0%
!! AAATAAATGGGTTGGGTGGG+GGG + Chr2:81373536-81373555 None:intergenic 45.0%
!! CAAATAAATGGGTTGGGTGG+GGG + Chr2:81373537-81373556 None:intergenic 45.0%
ATCAGCTTCATGTGCATCCC+TGG + Chr2:81373834-81373853 None:intergenic 50.0%
ATTAAGTCGGGCATCTGCGA+TGG + Chr2:81373262-81373281 None:intergenic 50.0%
TCAGCTTCATGTGCATCCCT+GGG + Chr2:81373833-81373852 None:intergenic 50.0%
! GAGGCGCCCTAAGATTTAAG+AGG + Chr2:81373863-81373882 None:intergenic 50.0%
AATACAGATCCCAACCGCCC+AGG - Chr2:81373813-81373832 MsG0280011240.01.T01:intron 55.0%
AGGTCACGTGCACATGTAGC+AGG + Chr2:81373909-81373928 None:intergenic 55.0%
ATACAGATCCCAACCGCCCA+GGG - Chr2:81373814-81373833 MsG0280011240.01.T01:intron 55.0%
TGGGCGGTTGGGATCTGTAT+TGG + Chr2:81373814-81373833 None:intergenic 55.0%
ATGTGCATCCCTGGGCGGTT+GGG + Chr2:81373825-81373844 None:intergenic 60.0%
GCTTCATGTGCATCCCTGGG+CGG + Chr2:81373830-81373849 None:intergenic 60.0%
CATGTGCATCCCTGGGCGGT+TGG + Chr2:81373826-81373845 None:intergenic 65.0%
CGGGCATCTGCGATGGCTTG+AGG + Chr2:81373255-81373274 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr2 gene 81373195 81374040 81373195 ID=MsG0280011240.01;Name=MsG0280011240.01
Chr2 mRNA 81373195 81374040 81373195 ID=MsG0280011240.01.T01;Parent=MsG0280011240.01;Name=MsG0280011240.01.T01;_AED=0.47;_eAED=0.57;_QI=0|1|0|1|1|1|2|0|137
Chr2 exon 81373848 81374040 81373848 ID=MsG0280011240.01.T01:exon:2743;Parent=MsG0280011240.01.T01
Chr2 exon 81373195 81373415 81373195 ID=MsG0280011240.01.T01:exon:2742;Parent=MsG0280011240.01.T01
Chr2 CDS 81373848 81374040 81373848 ID=MsG0280011240.01.T01:cds;Parent=MsG0280011240.01.T01
Chr2 CDS 81373195 81373415 81373195 ID=MsG0280011240.01.T01:cds;Parent=MsG0280011240.01.T01
Gene Sequence

>MsG0280011240.01.T01

ATGTCTTCTCTTCCAATTTTGAACAGTGTAAAGCATGTTTATGGCTATGAAGTTGTTCCTCAAGCCATCGCAGATGCCCGACTTAATGCCAAGCTAAATGGCATACAAAATGCAACATTTATTGAGGGGGATCTTAATAAAATTGATGGAAATTTCGGGAAGAATTTTCCTAAACCTGACATTGTTATTTCAGATCCCAACCGCCCAGGGATGCACATGAAGCTGATCAAGTTCCTCTTAAATCTTAGGGCGCCTCGCATAGTGTATGTTTCATGCAATCCTGCTACATGTGCACGTGACCTTGATTATCTCTGTCATGGTGTGGTATGTGTCATTTTAATTTTATTGTGTTTTAGCTCTATTTTTGAGATTCAACTATCTGAGTTGTTGAAGGTTTTGTGTAGGCAGAGCTGA

Protein sequence

>MsG0280011240.01.T01

MSSLPILNSVKHVYGYEVVPQAIADARLNAKLNGIQNATFIEGDLNKIDGNFGKNFPKPDIVISDPNRPGMHMKLIKFLLNLRAPRIVYVSCNPATCARDLDYLCHGVVCVILILLCFSSIFEIQLSELLKVLCRQS*