AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar zhongmu-4 / Msa1407770

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407770 MtrunA17_Chr8g0382231 98.710 310 4 0 1 310 321 630 0.0 640
Msa1407770 MtrunA17_Chr4g0050021 43.235 340 141 7 1 310 352 669 2.83e-75 243
Msa1407770 MtrunA17_Chr2g0279481 42.814 334 140 8 1 303 357 670 1.29e-74 242
Msa1407770 MtrunA17_Chr2g0303541 34.727 311 164 5 1 287 312 607 2.19e-54 187
Msa1407770 MtrunA17_Chr1g0195911 37.241 290 131 6 1 251 295 572 1.36e-50 176
Msa1407770 MtrunA17_Chr5g0401451 38.760 258 133 4 1 246 307 551 3.79e-50 175
Msa1407770 MtrunA17_Chr2g0285511 34.579 321 162 8 1 302 306 597 1.58e-49 173
Msa1407770 MtrunA17_Chr7g0268781 35.366 328 161 8 1 291 299 612 2.51e-49 173
Msa1407770 MtrunA17_Chr5g0401341 36.226 265 121 6 1 232 296 545 8.03e-48 169
Msa1407770 MtrunA17_Chr1g0181181 39.301 229 110 4 1 213 286 501 1.29e-46 165
Msa1407770 MtrunA17_Chr1g0199631 33.225 307 154 7 2 280 265 548 3.75e-46 164
Msa1407770 MtrunA17_Chr7g0245201 40.580 207 98 2 1 196 330 522 7.44e-45 161
Msa1407770 MtrunA17_Chr8g0374011 38.140 215 103 2 1 198 320 521 1.40e-44 160
Msa1407770 MtrunA17_Chr2g0320691 31.707 287 155 5 1 260 310 582 1.41e-44 160
Msa1407770 MtrunA17_Chr4g0010091 39.234 209 101 2 1 196 301 496 1.67e-44 160
Msa1407770 MtrunA17_Chr5g0412591 38.075 239 104 3 1 208 281 506 5.82e-44 158
Msa1407770 MtrunA17_Chr1g0207461 36.260 262 118 7 5 232 300 546 7.94e-43 155
Msa1407770 MtrunA17_Chr2g0332281 40.415 193 98 3 1 192 118 294 1.87e-42 151
Msa1407770 MtrunA17_Chr4g0003891 39.487 195 97 4 1 192 138 314 5.30e-41 148
Msa1407770 MtrunA17_Chr1g0150191 35.043 234 119 3 1 215 298 517 7.60e-40 147
Msa1407770 MtrunA17_Chr4g0053841 36.653 251 131 4 2 239 284 519 1.24e-39 146
Msa1407770 MtrunA17_Chr8g0367891 42.439 205 106 4 1 195 284 486 9.95e-38 141
Msa1407770 MtrunA17_Chr3g0108331 29.653 317 192 7 1 310 276 568 9.72e-36 135
Msa1407770 MtrunA17_Chr1g0191711 31.373 255 151 4 1 250 309 544 9.05e-35 132
Msa1407770 MtrunA17_Chr5g0432861 31.600 250 145 4 1 241 285 517 5.84e-34 130
Msa1407770 MtrunA17_Chr4g0076521 36.224 196 107 2 1 191 293 475 3.30e-32 125
Msa1407770 MtrunA17_Chr4g0037661 30.612 245 144 5 1 239 293 517 2.69e-27 111
Msa1407770 MtrunA17_Chr2g0290741 33.503 197 102 3 1 191 292 465 3.90e-25 105
Msa1407770 MtrunA17_Chr1g0163311 27.027 259 162 5 1 254 289 525 2.84e-24 102
Msa1407770 MtrunA17_Chr4g0023801 30.000 200 109 4 1 193 269 444 1.03e-23 101
Msa1407770 MtrunA17_Chr2g0280541 25.792 221 144 5 3 222 200 401 9.67e-19 86.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Msa1407770 AT5G10250.2 58.861 316 111 6 1 310 320 622 5.01e-110 332
Msa1407770 AT5G10250.1 56.646 316 103 6 1 310 320 607 1.02e-102 313
Msa1407770 AT5G64330.1 39.763 337 137 5 1 286 399 720 1.45e-70 233
Msa1407770 AT5G64330.3 46.667 225 78 2 1 196 340 551 3.68e-61 205
Msa1407770 AT5G64330.2 46.667 225 78 2 1 196 399 610 8.91e-61 205
Msa1407770 AT5G13600.1 34.936 312 158 7 1 295 308 591 6.37e-52 180
Msa1407770 AT1G03010.2 35.484 310 140 7 16 280 291 585 8.08e-48 169
Msa1407770 AT1G03010.1 35.484 310 140 7 16 280 318 612 8.46e-48 169
Msa1407770 AT1G03010.3 35.484 310 140 7 16 280 317 611 1.17e-47 169
Msa1407770 AT5G67385.4 41.709 199 99 2 1 194 246 432 1.42e-46 165
Msa1407770 AT5G67385.5 41.709 199 99 2 1 194 246 432 1.42e-46 165
Msa1407770 AT5G67385.2 41.709 199 99 2 1 194 246 432 1.42e-46 165
Msa1407770 AT5G67385.3 41.709 199 99 2 1 194 246 432 1.42e-46 165
Msa1407770 AT5G67385.1 41.709 199 99 2 1 194 291 477 1.70e-46 166
Msa1407770 AT1G30440.1 40.376 213 97 2 1 196 314 513 3.46e-46 166
Msa1407770 AT3G08660.3 33.223 301 165 6 1 285 222 502 1.37e-45 162
Msa1407770 AT3G08660.1 33.223 301 165 6 1 285 282 562 2.19e-45 162
Msa1407770 AT5G66560.4 40.796 201 101 3 1 196 309 496 2.19e-44 160
Msa1407770 AT3G44820.1 34.409 279 141 6 1 251 319 583 2.79e-44 160
Msa1407770 AT5G66560.1 40.796 201 101 3 1 196 348 535 2.87e-44 160
Msa1407770 AT5G66560.3 40.796 201 101 3 1 196 319 506 2.90e-44 160
Msa1407770 AT5G03250.2 34.459 296 156 8 1 279 198 472 7.37e-44 156
Msa1407770 AT2G47860.5 34.014 294 134 5 1 251 178 454 1.34e-43 156
Msa1407770 AT2G47860.2 34.014 294 134 5 1 251 178 454 1.34e-43 156
Msa1407770 AT5G48800.1 39.423 208 101 2 1 196 295 489 1.70e-43 157
Msa1407770 AT5G03250.1 34.459 296 156 8 1 279 310 584 2.97e-43 156
Msa1407770 AT2G47860.1 34.014 294 134 5 1 251 296 572 3.67e-43 157
Msa1407770 AT1G52770.1 33.333 270 143 6 1 252 128 378 3.82e-43 154
Msa1407770 AT2G47860.3 34.014 294 134 5 1 251 319 595 3.86e-43 157
Msa1407770 AT3G50840.2 32.566 304 176 9 1 299 251 530 2.83e-42 153
Msa1407770 AT3G50840.3 32.566 304 176 9 1 299 251 530 2.83e-42 153
Msa1407770 AT3G50840.1 32.566 304 176 9 1 299 287 566 6.00e-42 152
Msa1407770 AT2G14820.2 36.818 220 106 3 1 201 293 498 7.29e-41 150
Msa1407770 AT2G14820.1 36.818 220 106 3 1 201 293 498 7.29e-41 150
Msa1407770 AT3G08570.1 36.283 226 114 3 1 210 298 509 8.33e-40 147
Msa1407770 AT1G67900.5 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT1G67900.1 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT1G67900.3 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT1G67900.6 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT1G67900.2 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT1G67900.4 35.897 234 103 3 1 200 298 518 1.06e-39 147
Msa1407770 AT3G15570.1 38.021 192 104 2 1 192 133 309 1.97e-39 144
Msa1407770 AT5G67440.2 39.594 197 99 4 1 191 257 439 4.16e-37 139
Msa1407770 AT5G67440.3 39.594 197 99 4 1 191 294 476 7.85e-37 139
Msa1407770 AT5G67440.1 39.594 197 99 4 1 191 294 476 7.85e-37 139
Msa1407770 AT2G30520.2 36.190 210 109 3 1 200 236 430 1.14e-36 138
Msa1407770 AT2G30520.3 36.190 210 109 3 1 200 236 430 1.14e-36 138
Msa1407770 AT2G30520.1 36.190 210 109 3 1 200 284 478 1.15e-36 138
Msa1407770 AT4G37590.1 35.088 228 113 5 15 232 313 515 3.49e-36 137
Msa1407770 AT2G23050.2 38.764 178 80 2 18 195 190 338 1.01e-34 129
Msa1407770 AT2G23050.1 38.764 178 80 2 18 195 306 454 5.60e-34 130
Msa1407770 AT4G31820.2 36.181 199 92 4 1 191 244 415 4.67e-33 127
Msa1407770 AT4G31820.1 36.181 199 92 4 1 191 297 468 8.99e-33 127
Msa1407770 AT3G26490.1 33.480 227 105 3 1 195 299 511 2.58e-32 126
Msa1407770 AT3G26490.3 33.480 227 105 3 1 195 299 511 2.58e-32 126
Msa1407770 AT3G26490.4 33.480 227 105 3 1 195 299 511 2.58e-32 126
Msa1407770 AT3G49970.1 37.640 178 87 4 1 176 239 394 2.96e-32 125
Msa1407770 AT3G49970.2 37.640 178 87 4 1 176 239 394 2.96e-32 125
Msa1407770 AT3G19850.1 34.715 193 106 4 1 190 289 464 5.90e-30 119
Msa1407770 AT5G47800.3 31.633 196 114 2 1 189 292 474 1.16e-28 115
Msa1407770 AT5G47800.1 31.633 196 114 2 1 189 292 474 1.16e-28 115
Msa1407770 AT5G47800.4 31.633 196 114 2 1 189 292 474 1.16e-28 115
Msa1407770 AT5G47800.2 32.143 196 113 2 1 189 405 587 2.26e-28 115
Msa1407770 AT1G50280.2 31.313 198 118 2 1 198 256 435 2.77e-25 105
Msa1407770 AT1G50280.1 31.313 198 118 2 1 198 289 468 2.87e-25 105
Msa1407770 AT3G03510.1 29.134 254 146 8 53 300 223 448 8.15e-22 95.5
Msa1407770 AT3G08660.2 35.256 156 79 2 1 148 282 423 1.15e-21 95.1
Msa1407770 AT2G47860.6 35.152 165 67 3 1 141 319 467 1.47e-19 89.0
Msa1407770 AT2G47860.4 35.152 165 67 3 1 141 296 444 1.83e-19 88.6
Msa1407770 AT5G66560.2 35.172 145 76 3 1 140 348 479 4.84e-18 84.7
Msa1407770 AT3G22104.1 29.545 176 104 2 1 176 265 420 5.92e-18 84.3
Msa1407770 AT3G22104.4 29.545 176 104 2 1 176 190 345 6.66e-18 84.0
Msa1407770 AT3G22104.2 29.545 176 104 2 1 176 190 345 6.66e-18 84.0
Msa1407770 AT3G22104.3 29.545 176 104 2 1 176 190 345 6.66e-18 84.0
Msa1407770 AT5G17580.2 31.884 138 90 2 59 193 323 459 1.11e-17 84.0
Msa1407770 AT5G17580.1 31.884 138 90 2 59 193 308 444 1.40e-17 83.6
Msa1407770 AT5G48130.1 28.058 139 99 1 60 198 339 476 6.67e-17 81.6
Msa1407770 AT3G26490.2 28.902 173 77 3 1 141 299 457 7.63e-13 69.3

Find 136 sgRNAs with CRISPR-Local

Find 260 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTTCTGTCAGACTGACTTTC+AGG 0.132980 tig0023324:-53056 None:intergenic
CACAGCATCTTCTAACAATT+TGG 0.179939 tig0023324:-54347 None:intergenic
CTCCAGCCTGATAGATTCTT+TGG 0.216318 tig0023324:-56666 None:intergenic
ATTCAATATTAGTAGGCTTT+TGG 0.241298 tig0023324:+55191 Msa1407770:CDS
CATTTGTCGTGCTTTCTTCA+TGG 0.250887 tig0023324:+54265 Msa1407770:five_prime_UTR
CAGCCTCTAATCTCAAATTC+TGG 0.250919 tig0023324:+54051 Msa1407770:five_prime_UTR
TCTGGTTTCATATATCATTT+AGG 0.251520 tig0023324:+52941 Msa1407770:five_prime_UTR
TTAGATATAATATAAAATTT+AGG 0.251980 tig0023324:-56887 None:intergenic
TGCGGATAATTTCAGCAATT+AGG 0.253941 tig0023324:+54481 Msa1407770:five_prime_UTR
AACATTGTAATTGAAAGATT+TGG 0.262115 tig0023324:-55243 None:intergenic
TGAGGAGGAATTATGCTTAT+AGG 0.262129 tig0023324:-54701 None:intergenic
TTCCCAGAATTTGAGATTAG+AGG 0.277466 tig0023324:-54054 None:intergenic
AAGCTGGTGGTTCGATGATT+TGG 0.289085 tig0023324:+54434 Msa1407770:five_prime_UTR
GACTTTCAGGGTCTTGTGCT+TGG 0.289224 tig0023324:-53043 None:intergenic
GCGGATAATTTCAGCAATTA+GGG 0.291903 tig0023324:+54482 Msa1407770:five_prime_UTR
TTAGAAGGGCTTAGAGGATA+TGG 0.292438 tig0023324:+54585 Msa1407770:five_prime_UTR
CTCAAGCTTGAAAACTTTCC+AGG 0.304064 tig0023324:+54081 Msa1407770:five_prime_UTR
TATCAGGCTGGAGTTTAAAT+TGG 0.321879 tig0023324:+56676 Msa1407770:CDS
GCACATTATAAGTGGTTTCC+TGG 0.326824 tig0023324:-53359 None:intergenic
TGTCTATAGTTTCAGATGTC+TGG 0.334509 tig0023324:-56533 None:intergenic
ACCTGGACAACTGTTCTTAG+AGG 0.338185 tig0023324:-55560 None:intergenic
ACTTTCAGGGTCTTGTGCTT+GGG 0.351122 tig0023324:-53042 None:intergenic
CCTACTAATATTGAATTTCC+CGG 0.364748 tig0023324:-55184 None:intergenic
ACAGTACTCAGCATTAATTT+AGG 0.366320 tig0023324:+52911 Msa1407770:five_prime_UTR
GAGGAGTTAGAAGGGCTTAG+AGG 0.370662 tig0023324:+54579 Msa1407770:five_prime_UTR
AAATTTAGGCTATATGCTTC+AGG 0.374622 tig0023324:-56873 None:intergenic
ACAATTCTAAAATTCTGTTA+TGG 0.376842 tig0023324:+54123 Msa1407770:five_prime_UTR
TGCAGAGTCTTCTACGGTCT+TGG 0.377991 tig0023324:-55472 None:intergenic
TTCTACGGTCTTGGTCAGTT+AGG 0.381517 tig0023324:-55463 None:intergenic
CCGGGAAATTCAATATTAGT+AGG 0.395757 tig0023324:+55184 Msa1407770:CDS
TTTACCAATGATCTCTGGTT+TGG 0.398294 tig0023324:-54514 None:intergenic
GATGCTGCATCCTCACTTGC+GGG 0.402710 tig0023324:-54402 None:intergenic
GACAAACCAAAGAATCTATC+AGG 0.413587 tig0023324:+56660 Msa1407770:CDS
ACACGGAGAGCCTTCTTCTC+GGG 0.418325 tig0023324:-56800 None:intergenic
GAGCTAATTGCTGCAAGCTT+GGG 0.418641 tig0023324:-52990 None:intergenic
TCTAACTCCTCATACATTCC+TGG 0.422868 tig0023324:-54567 None:intergenic
GACCTTTAGGTAGGTGTCAA+TGG 0.427446 tig0023324:-55328 None:intergenic
TTAATTCCTAGATATCAAAA+TGG 0.429080 tig0023324:+54860 Msa1407770:CDS
AGCCTCTAATCTCAAATTCT+GGG 0.431924 tig0023324:+54052 Msa1407770:five_prime_UTR
ATGCAACTGCAACAAAAGTC+CGG 0.435207 tig0023324:+55165 Msa1407770:CDS
ATTAATTTAGGCAGCGTGTC+TGG 0.435511 tig0023324:+52923 Msa1407770:five_prime_UTR
GACACGGAGAGCCTTCTTCT+CGG 0.443418 tig0023324:-56801 None:intergenic
TTATCCGAGTTGAAGTCTAT+AGG 0.445995 tig0023324:-54150 None:intergenic
TATCCGAGTTGAAGTCTATA+GGG 0.446756 tig0023324:-54149 None:intergenic
CTTGAATTGAAAAGTCTGTT+GGG 0.449484 tig0023324:-53335 None:intergenic
TTCTGTCAGACTGACTTTCA+GGG 0.449988 tig0023324:-53055 None:intergenic
TCTACGGTCTTGGTCAGTTA+GGG 0.449995 tig0023324:-55462 None:intergenic
TCCTTCCATGTAAAACCAAC+AGG 0.451380 tig0023324:+56714 Msa1407770:CDS
ATACATTTACCAATGATCTC+TGG 0.452219 tig0023324:-54519 None:intergenic
AAGTGGAATCAACTCTCAAC+AGG 0.453660 tig0023324:+56503 Msa1407770:CDS
TGAGCTAATTGCTGCAAGCT+TGG 0.454672 tig0023324:-52991 None:intergenic
TCTCTAGTTTCACCTCCTGT+TGG 0.456759 tig0023324:-56729 None:intergenic
TACCTTGTGCACATTATAAG+TGG 0.465167 tig0023324:-53367 None:intergenic
GGAATGTATGAGGAGTTAGA+AGG 0.465676 tig0023324:+54570 Msa1407770:five_prime_UTR
CTTGGTCAGTTAGGGAAGGA+TGG 0.466551 tig0023324:-55454 None:intergenic
AGCTATTGTGACATGTTTGT+TGG 0.475232 tig0023324:-53111 None:intergenic
TGAAATGATCGAGTATGTTC+TGG 0.476508 tig0023324:-55288 None:intergenic
ACTTGAATTGAAAAGTCTGT+TGG 0.476835 tig0023324:-53336 None:intergenic
ATTTCAGCAATTAGGGCAAA+GGG 0.478066 tig0023324:+54489 Msa1407770:five_prime_UTR
ATATCAAAATGCGGCTACAT+AGG 0.478687 tig0023324:+54015 Msa1407770:five_prime_UTR
ATCTGCAACAAGAACTTACA+TGG 0.493501 tig0023324:-54737 None:intergenic
GCCTCTAAGAACAGTTGTCC+AGG 0.494899 tig0023324:+55559 Msa1407770:CDS
GCTATTGTGACATGTTTGTT+GGG 0.495372 tig0023324:-53110 None:intergenic
ACGAGTGTGGTTTGGTGATG+TGG 0.497567 tig0023324:-56766 None:intergenic
GATGCTGTGATTCTATAGCT+TGG 0.503818 tig0023324:+54361 Msa1407770:five_prime_UTR
TGTGAGGAAAGCTTCAGTCT+TGG 0.507277 tig0023324:-54244 None:intergenic
GGAACCAAACCAGAGATCAT+TGG 0.508422 tig0023324:+54510 Msa1407770:five_prime_UTR
GATCTACGACGAGTGTGGTT+TGG 0.513023 tig0023324:-56774 None:intergenic
CTTGTTGCAGATGCTAAAGA+TGG 0.513993 tig0023324:+54748 Msa1407770:exon
GAAAAGTCTGTTGGGATTCG+AGG 0.514401 tig0023324:-53327 None:intergenic
AGCAATGCAAGAGAAGGAAC+CGG 0.515645 tig0023324:+56476 Msa1407770:CDS
CATTCCTGGTAGCCATCTCT+TGG 0.516344 tig0023324:-54553 None:intergenic
CTGTGATTCTATAGCTTGGA+AGG 0.517789 tig0023324:+54365 Msa1407770:five_prime_UTR
TCAGACCTTGAAAAGAGAGT+TGG 0.522254 tig0023324:+54803 Msa1407770:CDS
GTGTAGAATTGTAGTTAAGA+TGG 0.523176 tig0023324:-52878 None:intergenic
CAGGCTGGAGTTTAAATTGG+CGG 0.523456 tig0023324:+56679 Msa1407770:CDS
TAGAAGGGCTTAGAGGATAT+GGG 0.526409 tig0023324:+54586 Msa1407770:five_prime_UTR
GTAGATCAGCCCCGAGAAGA+AGG 0.527618 tig0023324:+56790 Msa1407770:CDS
CTTGTGAAAATCTATCTCCA+TGG 0.528028 tig0023324:+54313 Msa1407770:five_prime_UTR
CCTTGAAAAGAGAGTTGGTA+TGG 0.528846 tig0023324:+54808 Msa1407770:CDS
AACCAAAGAATCTATCAGGC+TGG 0.531250 tig0023324:+56664 Msa1407770:CDS
AGATATCAAAATGGAGGTCA+AGG 0.533332 tig0023324:+54869 Msa1407770:CDS
TGCAACTGCAACAAAAGTCC+GGG 0.536493 tig0023324:+55166 Msa1407770:CDS
TTGGTCAGTTAGGGAAGGAT+GGG 0.536654 tig0023324:-55453 None:intergenic
AATTTCAGCAATTAGGGCAA+AGG 0.537477 tig0023324:+54488 Msa1407770:five_prime_UTR
AAAAGTCTGTTGGGATTCGA+GGG 0.537749 tig0023324:-53326 None:intergenic
AAACATGAAATCACTTCAAG+TGG 0.548709 tig0023324:+53019 Msa1407770:five_prime_UTR
AGATGCTGCATCCTCACTTG+CGG 0.555801 tig0023324:-54403 None:intergenic
TTGTGAAAATCTATCTCCAT+GGG 0.559597 tig0023324:+54314 Msa1407770:five_prime_UTR
TAAAAGGCTGACCTTTAGGT+AGG 0.562453 tig0023324:-55337 None:intergenic
GAGAGCAGCAATGCAAGAGA+AGG 0.563979 tig0023324:+56470 Msa1407770:CDS
TATGCCAAGAGATGGCTACC+AGG 0.565928 tig0023324:+54549 Msa1407770:five_prime_UTR
ATGACTGAAGAACTAGAAGA+TGG 0.572238 tig0023324:+54210 Msa1407770:five_prime_UTR
GATCTGTTGCAACTTCAGCT+AGG 0.573474 tig0023324:-55221 None:intergenic
TAGAATTGTAGTTAAGATGG+TGG 0.574829 tig0023324:-52875 None:intergenic
CAGCATCTAATCAAGAAAGC+TGG 0.575400 tig0023324:+54418 Msa1407770:five_prime_UTR
GTTCTGCAGTTCTACCATGT+TGG 0.579053 tig0023324:-56592 None:intergenic
GAGTTGATTCCACTTTGTGC+CGG 0.581941 tig0023324:-56495 None:intergenic
CACGGAGAGCCTTCTTCTCG+GGG 0.584375 tig0023324:-56799 None:intergenic
ATGGCTACCAGGAATGTATG+AGG 0.585069 tig0023324:+54560 Msa1407770:five_prime_UTR
TCAAATGTTTCACATCCACC+TGG 0.585855 tig0023324:-54099 None:intergenic
AGCCATTGACACCTACCTAA+AGG 0.587167 tig0023324:+55326 Msa1407770:CDS
TCATGCAATATGCCAAGAGA+TGG 0.594644 tig0023324:+54541 Msa1407770:five_prime_UTR
CATGGGACAGCATCTTGTTG+AGG 0.601532 tig0023324:-54719 None:intergenic
AGAGAGCACTAGACATAGCA+AGG 0.603854 tig0023324:+54656 Msa1407770:five_prime_UTR
CGAGTGTGGTTTGGTGATGT+GGG 0.605082 tig0023324:-56765 None:intergenic
GGGCTTAGAGGATATGGGCA+TGG 0.605115 tig0023324:+54591 Msa1407770:five_prime_UTR
GAATGTATGAGGAGTTAGAA+GGG 0.605950 tig0023324:+54571 Msa1407770:five_prime_UTR
CGGTCTTGGTCAGTTAGGGA+AGG 0.612259 tig0023324:-55458 None:intergenic
CGAGAATGTAAAGTCCAACA+TGG 0.615865 tig0023324:+56578 Msa1407770:CDS
CATAAAATGATCAATGCGGA+GGG 0.617020 tig0023324:-54460 None:intergenic
GGGACAGCATCTTGTTGAGG+AGG 0.618679 tig0023324:-54716 None:intergenic
GAGAAGGAACCGGCACAAAG+TGG 0.620698 tig0023324:+56486 Msa1407770:CDS
ATTCCTAGATATCAAAATGG+AGG 0.624406 tig0023324:+54863 Msa1407770:CDS
AAAATGATCAATGCGGAGGG+TGG 0.627548 tig0023324:-54457 None:intergenic
CAAAATGCGGCTACATAGGA+CGG 0.632260 tig0023324:+54019 Msa1407770:five_prime_UTR
AGAATTGTAGTTAAGATGGT+GGG 0.638014 tig0023324:-52874 None:intergenic
ATGCGGCTACATAGGACGGT+CGG 0.639956 tig0023324:+54023 Msa1407770:five_prime_UTR
GCATAAAATGATCAATGCGG+AGG 0.642568 tig0023324:-54461 None:intergenic
ACAGGAGGTGAAACTAGAGA+TGG 0.647018 tig0023324:+56732 Msa1407770:CDS
AAGAAAGCACGACAAATGTG+AGG 0.659530 tig0023324:-54260 None:intergenic
AAGCTTGAAAACTTTCCAGG+TGG 0.660259 tig0023324:+54084 Msa1407770:five_prime_UTR
CATCTAATCAAGAAAGCTGG+TGG 0.660897 tig0023324:+54421 Msa1407770:five_prime_UTR
AACCACTTATAATGTGCACA+AGG 0.667560 tig0023324:+53365 Msa1407770:five_prime_UTR
CCTCCCTATAGACTTCAACT+CGG 0.675286 tig0023324:+54146 Msa1407770:five_prime_UTR
GGGCTGATCTACGACGAGTG+TGG 0.675575 tig0023324:-56779 None:intergenic
TAATGCTGAGTACTGTTCTG+TGG 0.676472 tig0023324:-52904 None:intergenic
CCGAGTTGAAGTCTATAGGG+AGG 0.687521 tig0023324:-54146 None:intergenic
TCTGCAACAAGAACTTACAT+GGG 0.692050 tig0023324:-54736 None:intergenic
AAAGTCTGTTGGGATTCGAG+GGG 0.692715 tig0023324:-53325 None:intergenic
TTCCATGTAAAACCAACAGG+AGG 0.698959 tig0023324:+56717 Msa1407770:CDS
TACCCCTTGATATCAAAATG+CGG 0.703247 tig0023324:+54006 Msa1407770:five_prime_UTR
TCCGCATAAAATGATCAATG+CGG 0.719094 tig0023324:-54464 None:intergenic
TATGGTGTTAGAAGATGCTG+AGG 0.726855 tig0023324:+54826 Msa1407770:CDS
CATACTCGATCATTTCACGA+TGG 0.744904 tig0023324:+55294 Msa1407770:CDS
AAGACATAGATGTAGTACAA+CGG 0.746121 tig0023324:+55106 Msa1407770:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TTCAATTATCTTATTAAAAA+TGG - tig0023324:56247-56266 None:intergenic 10.0%
!! TTTAACTGCATAAAATATAA+AGG - tig0023324:53182-53201 None:intergenic 15.0%
!!! TATTTTCTCTGTTTATTTTT+TGG + tig0023324:53279-53298 Msa1407770:intron 15.0%
!!! TTTATATTTTTCCATTTGTA+AGG - tig0023324:55948-55967 None:intergenic 15.0%
!!! ATATAAAGGATTCTATTTCT+GGG - tig0023324:53168-53187 None:intergenic 20.0%
!! AATATAAAGGATTCTATTTC+TGG - tig0023324:53169-53188 None:intergenic 20.0%
!! ACTCATACAAAAATAACTAT+TGG - tig0023324:53255-53274 None:intergenic 20.0%
!!! AATGATTTTCTGCATTTATA+AGG - tig0023324:53411-53430 None:intergenic 20.0%
!!! TGGTTTTCAAAAAAATTCTA+AGG - tig0023324:53870-53889 None:intergenic 20.0%
!! CAACATATAAAAGATTAAGT+TGG - tig0023324:53967-53986 None:intergenic 20.0%
!!! CAACTTAATCTTTTATATGT+TGG + tig0023324:53965-53984 Msa1407770:intron 20.0%
!!! ACAATTCTAAAATTCTGTTA+TGG + tig0023324:54123-54142 Msa1407770:five_prime_UTR 20.0%
!! TTAATTCCTAGATATCAAAA+TGG + tig0023324:54860-54879 Msa1407770:CDS 20.0%
!! TGCGAAAAATAAAAAATAAC+CGG + tig0023324:54977-54996 Msa1407770:intron 20.0%
!! AACATTGTAATTGAAAGATT+TGG - tig0023324:55246-55265 None:intergenic 20.0%
!! ACAAGAAAATAGGAATTAAA+AGG - tig0023324:55356-55375 None:intergenic 20.0%
!!! GATTTCTTTTTCAAAGTTAA+AGG - tig0023324:55821-55840 None:intergenic 20.0%
!!! TTTTATTTCTAAATCACAGT+AGG - tig0023324:56106-56125 None:intergenic 20.0%
!! TATCTTATTAAAAATGGAGT+TGG - tig0023324:56241-56260 None:intergenic 20.0%
!!! GATGAAATAATTTTAAGACA+CGG - tig0023324:56820-56839 None:intergenic 20.0%
!! TCTGGTTTCATATATCATTT+AGG + tig0023324:52941-52960 Msa1407770:five_prime_UTR 25.0%
! GCATAAAAAATGAACATTCA+AGG + tig0023324:53138-53157 Msa1407770:five_prime_UTR 25.0%
!!! TCTCTGTTTATTTTTTGGTT+TGG + tig0023324:53284-53303 Msa1407770:intron 25.0%
!!! CTCTGTTTATTTTTTGGTTT+GGG + tig0023324:53285-53304 Msa1407770:intron 25.0%
!!! CCATGTTTTTATTCTAATTC+TGG - tig0023324:53890-53909 None:intergenic 25.0%
! CCAGAATTAGAATAAAAACA+TGG + tig0023324:53887-53906 Msa1407770:intron 25.0%
!!! TTTTTTATTTTTCGCAGAGT+TGG - tig0023324:54973-54992 None:intergenic 25.0%
! GAAAAATAAAAAATAACCGG+AGG + tig0023324:54980-54999 Msa1407770:intron 25.0%
! AAGATAGAAATTAGTGTCTT+TGG - tig0023324:55019-55038 None:intergenic 25.0%
!! ATTCAATATTAGTAGGCTTT+TGG + tig0023324:55191-55210 Msa1407770:CDS 25.0%
! TGAATTGAACACAAGAAAAT+AGG - tig0023324:55366-55385 None:intergenic 25.0%
!! TATTTTCTTGTGTTCAATTC+AGG + tig0023324:55365-55384 Msa1407770:intron 25.0%
! GGAATTCTTAAAACTCTATT+TGG - tig0023324:55642-55661 None:intergenic 25.0%
!!! TGTCATCATTTTTTTCTTCA+TGG - tig0023324:55663-55682 None:intergenic 25.0%
! AAAGAAGAAACTTCTTTAGA+AGG + tig0023324:55884-55903 Msa1407770:intron 25.0%
AGAATTGTAGTTAAGATGGT+GGG - tig0023324:52877-52896 None:intergenic 30.0%
TAGAATTGTAGTTAAGATGG+TGG - tig0023324:52878-52897 None:intergenic 30.0%
GTGTAGAATTGTAGTTAAGA+TGG - tig0023324:52881-52900 None:intergenic 30.0%
ACAGTACTCAGCATTAATTT+AGG + tig0023324:52911-52930 Msa1407770:five_prime_UTR 30.0%
AAACATGAAATCACTTCAAG+TGG + tig0023324:53019-53038 Msa1407770:five_prime_UTR 30.0%
CTTGAATTGAAAAGTCTGTT+GGG - tig0023324:53338-53357 None:intergenic 30.0%
ACTTGAATTGAAAAGTCTGT+TGG - tig0023324:53339-53358 None:intergenic 30.0%
! TTTTACTGAGCTAAACAAGA+AGG - tig0023324:53436-53455 None:intergenic 30.0%
TTGTGAAAATCTATCTCCAT+GGG + tig0023324:54314-54333 Msa1407770:five_prime_UTR 30.0%
ATACATTTACCAATGATCTC+TGG - tig0023324:54522-54541 None:intergenic 30.0%
ATTCCTAGATATCAAAATGG+AGG + tig0023324:54863-54882 Msa1407770:CDS 30.0%
!! TCATTTTTCTCCTGTCATAT+TGG + tig0023324:54920-54939 Msa1407770:intron 30.0%
AAGACATAGATGTAGTACAA+CGG + tig0023324:55106-55125 Msa1407770:CDS 30.0%
CCTACTAATATTGAATTTCC+CGG - tig0023324:55187-55206 None:intergenic 30.0%
!! TTATTTTGCAGAGTCTTCTA+CGG - tig0023324:55481-55500 None:intergenic 30.0%
!! TGTGTTGATGTGTTTTTCAA+AGG - tig0023324:55730-55749 None:intergenic 30.0%
ATTGAAGATCTGAACCAATT+TGG + tig0023324:55750-55769 Msa1407770:intron 30.0%
TTGAAGATCTGAACCAATTT+GGG + tig0023324:55751-55770 Msa1407770:intron 30.0%
! AAACTTCTTTAGAAGGAAAG+AGG + tig0023324:55891-55910 Msa1407770:intron 30.0%
! AACTTCTTTAGAAGGAAAGA+GGG + tig0023324:55892-55911 Msa1407770:intron 30.0%
GTACAAATGATCCTTACAAA+TGG + tig0023324:55934-55953 Msa1407770:intron 30.0%
! ACTCTATGACACATGATTTT+GGG - tig0023324:56012-56031 None:intergenic 30.0%
TGTATAGAGGAAATACTTTG+CGG - tig0023324:56061-56080 None:intergenic 30.0%
! ACACAAACTTTTGTGTATAG+AGG - tig0023324:56074-56093 None:intergenic 30.0%
AATTCCACAAAACAACATTG+TGG + tig0023324:56265-56284 Msa1407770:intron 30.0%
AAATTTAGGCTATATGCTTC+AGG - tig0023324:56876-56895 None:intergenic 30.0%
GCTATTGTGACATGTTTGTT+GGG - tig0023324:53113-53132 None:intergenic 35.0%
AGCTATTGTGACATGTTTGT+TGG - tig0023324:53114-53133 None:intergenic 35.0%
! AGACTTTTCAATTCAAGTCC+AGG + tig0023324:53341-53360 Msa1407770:five_prime_UTR 35.0%
AACCACTTATAATGTGCACA+AGG + tig0023324:53365-53384 Msa1407770:five_prime_UTR 35.0%
TACCTTGTGCACATTATAAG+TGG - tig0023324:53370-53389 None:intergenic 35.0%
! AAGAGCACAATCCTGTTAAT+AGG + tig0023324:53563-53582 Msa1407770:intron 35.0%
AGAGCACAATCCTGTTAATA+GGG + tig0023324:53564-53583 Msa1407770:intron 35.0%
GATTTATGCTCCCTATTAAC+AGG - tig0023324:53577-53596 None:intergenic 35.0%
! CTCTTTTGGATAGAGAAAGA+GGG + tig0023324:53680-53699 Msa1407770:intron 35.0%
! TCTTTTGGATAGAGAAAGAG+GGG + tig0023324:53681-53700 Msa1407770:intron 35.0%
!! TGTTTTGCATGCTTTTAAGG+AGG - tig0023324:53760-53779 None:intergenic 35.0%
!!! TGCTGTTTTGCATGCTTTTA+AGG - tig0023324:53763-53782 None:intergenic 35.0%
TACCCCTTGATATCAAAATG+CGG + tig0023324:54006-54025 Msa1407770:five_prime_UTR 35.0%
!! TAGCCGCATTTTGATATCAA+GGG - tig0023324:54012-54031 None:intergenic 35.0%
ATATCAAAATGCGGCTACAT+AGG + tig0023324:54015-54034 Msa1407770:five_prime_UTR 35.0%
AGCCTCTAATCTCAAATTCT+GGG + tig0023324:54052-54071 Msa1407770:five_prime_UTR 35.0%
! TTCCCAGAATTTGAGATTAG+AGG - tig0023324:54057-54076 None:intergenic 35.0%
! TATCCGAGTTGAAGTCTATA+GGG - tig0023324:54152-54171 None:intergenic 35.0%
! TTATCCGAGTTGAAGTCTAT+AGG - tig0023324:54153-54172 None:intergenic 35.0%
ATGACTGAAGAACTAGAAGA+TGG + tig0023324:54210-54229 Msa1407770:five_prime_UTR 35.0%
CTTGTGAAAATCTATCTCCA+TGG + tig0023324:54313-54332 Msa1407770:five_prime_UTR 35.0%
! ATTTGGATATTTTCAGCCCA+TGG - tig0023324:54333-54352 None:intergenic 35.0%
CACAGCATCTTCTAACAATT+TGG - tig0023324:54350-54369 None:intergenic 35.0%
CATAAAATGATCAATGCGGA+GGG - tig0023324:54463-54482 None:intergenic 35.0%
!! TCCGCATTGATCATTTTATG+CGG + tig0023324:54463-54482 Msa1407770:five_prime_UTR 35.0%
TCCGCATAAAATGATCAATG+CGG - tig0023324:54467-54486 None:intergenic 35.0%
TGCGGATAATTTCAGCAATT+AGG + tig0023324:54481-54500 Msa1407770:five_prime_UTR 35.0%
GCGGATAATTTCAGCAATTA+GGG + tig0023324:54482-54501 Msa1407770:five_prime_UTR 35.0%
AATTTCAGCAATTAGGGCAA+AGG + tig0023324:54488-54507 Msa1407770:five_prime_UTR 35.0%
ATTTCAGCAATTAGGGCAAA+GGG + tig0023324:54489-54508 Msa1407770:five_prime_UTR 35.0%
TTTACCAATGATCTCTGGTT+TGG - tig0023324:54517-54536 None:intergenic 35.0%
GAATGTATGAGGAGTTAGAA+GGG + tig0023324:54571-54590 Msa1407770:five_prime_UTR 35.0%
TGAGGAGGAATTATGCTTAT+AGG - tig0023324:54704-54723 None:intergenic 35.0%
TCTGCAACAAGAACTTACAT+GGG - tig0023324:54739-54758 None:intergenic 35.0%
ATCTGCAACAAGAACTTACA+TGG - tig0023324:54740-54759 None:intergenic 35.0%
!! TGACCTCCATTTTGATATCT+AGG - tig0023324:54869-54888 None:intergenic 35.0%
AGATATCAAAATGGAGGTCA+AGG + tig0023324:54869-54888 Msa1407770:CDS 35.0%
AAATGGAGGTCAAGGAAAAA+TGG + tig0023324:54877-54896 Msa1407770:CDS 35.0%
TTCTCCTGTCATATTGGAAT+TGG + tig0023324:54926-54945 Msa1407770:intron 35.0%
AATGCCAATTCCAATATGAC+AGG - tig0023324:54933-54952 None:intergenic 35.0%
CCGGGAAATTCAATATTAGT+AGG + tig0023324:55184-55203 Msa1407770:CDS 35.0%
TGAAATGATCGAGTATGTTC+TGG - tig0023324:55291-55310 None:intergenic 35.0%
GAATTAAAAGGCTGACCTTT+AGG - tig0023324:55344-55363 None:intergenic 35.0%
!! GATCTTTGGAACTTAGTCTT+CGG + tig0023324:55594-55613 Msa1407770:intron 35.0%
TGAAGATCTGAACCAATTTG+GGG + tig0023324:55752-55771 Msa1407770:intron 35.0%
AACCAAACAGCAAGACTAAT+TGG + tig0023324:55966-55985 Msa1407770:intron 35.0%
!! TTCCAATTAGTCTTGCTGTT+TGG - tig0023324:55971-55990 None:intergenic 35.0%
! CTCTATGACACATGATTTTG+GGG - tig0023324:56011-56030 None:intergenic 35.0%
! CACTCTATGACACATGATTT+TGG - tig0023324:56013-56032 None:intergenic 35.0%
ATCATGTGTCATAGAGTGAA+AGG + tig0023324:56015-56034 Msa1407770:intron 35.0%
TCATGTGTCATAGAGTGAAA+GGG + tig0023324:56016-56035 Msa1407770:intron 35.0%
!!! AGATCCACAATGTTGTTTTG+TGG - tig0023324:56272-56291 None:intergenic 35.0%
ACAAAACAACATTGTGGATC+TGG + tig0023324:56271-56290 Msa1407770:intron 35.0%
! TCTGGTTCACATTATAGACA+TGG + tig0023324:56289-56308 Msa1407770:intron 35.0%
!! TGGACATATTAGCTCTGAAA+AGG - tig0023324:56372-56391 None:intergenic 35.0%
CAGAGCTAATATGTCCATAA+TGG + tig0023324:56375-56394 Msa1407770:intron 35.0%
TCTAAGCTGAATCACCATTA+TGG - tig0023324:56392-56411 None:intergenic 35.0%
TGTCTATAGTTTCAGATGTC+TGG - tig0023324:56536-56555 None:intergenic 35.0%
GACAAACCAAAGAATCTATC+AGG + tig0023324:56660-56679 Msa1407770:CDS 35.0%
TATCAGGCTGGAGTTTAAAT+TGG + tig0023324:56676-56695 Msa1407770:CDS 35.0%
! TAATGCTGAGTACTGTTCTG+TGG - tig0023324:52907-52926 None:intergenic 40.0%
ATTAATTTAGGCAGCGTGTC+TGG + tig0023324:52923-52942 Msa1407770:five_prime_UTR 40.0%
! TTCTGTCAGACTGACTTTCA+GGG - tig0023324:53058-53077 None:intergenic 40.0%
!!! TTTTTGGTTTGGGCTTATGC+AGG + tig0023324:53295-53314 Msa1407770:intron 40.0%
AAAAGTCTGTTGGGATTCGA+GGG - tig0023324:53329-53348 None:intergenic 40.0%
GCACATTATAAGTGGTTTCC+TGG - tig0023324:53362-53381 None:intergenic 40.0%
TTCCAAACAGTATCATCAGC+AGG + tig0023324:53459-53478 Msa1407770:intron 40.0%
TCCAAACAGTATCATCAGCA+GGG + tig0023324:53460-53479 Msa1407770:intron 40.0%
TATCATCAGCAGGGTATATG+AGG + tig0023324:53469-53488 Msa1407770:intron 40.0%
ATCATCAGCAGGGTATATGA+GGG + tig0023324:53470-53489 Msa1407770:intron 40.0%
CTTTCTCTATCCAAAAGAGG+TGG - tig0023324:53679-53698 None:intergenic 40.0%
CCTCTTTCTCTATCCAAAAG+AGG - tig0023324:53682-53701 None:intergenic 40.0%
! CCTCTTTTGGATAGAGAAAG+AGG + tig0023324:53679-53698 Msa1407770:intron 40.0%
! CTTTTGGATAGAGAAAGAGG+GGG + tig0023324:53682-53701 Msa1407770:intron 40.0%
TAGACAGAGAACATCAGACA+TGG + tig0023324:53725-53744 Msa1407770:intron 40.0%
AGAGAACATCAGACATGGAT+AGG + tig0023324:53730-53749 Msa1407770:intron 40.0%
GAGAACATCAGACATGGATA+GGG + tig0023324:53731-53750 Msa1407770:intron 40.0%
!! AGCCGCATTTTGATATCAAG+GGG - tig0023324:54011-54030 None:intergenic 40.0%
!! GTAGCCGCATTTTGATATCA+AGG - tig0023324:54013-54032 None:intergenic 40.0%
CAGCCTCTAATCTCAAATTC+TGG + tig0023324:54051-54070 Msa1407770:five_prime_UTR 40.0%
CTCAAGCTTGAAAACTTTCC+AGG + tig0023324:54081-54100 Msa1407770:five_prime_UTR 40.0%
AAGCTTGAAAACTTTCCAGG+TGG + tig0023324:54084-54103 Msa1407770:five_prime_UTR 40.0%
TCAAATGTTTCACATCCACC+TGG - tig0023324:54102-54121 None:intergenic 40.0%
! AAGAAAGCACGACAAATGTG+AGG - tig0023324:54263-54282 None:intergenic 40.0%
CATTTGTCGTGCTTTCTTCA+TGG + tig0023324:54265-54284 Msa1407770:five_prime_UTR 40.0%
GATGCTGTGATTCTATAGCT+TGG + tig0023324:54361-54380 Msa1407770:five_prime_UTR 40.0%
CTGTGATTCTATAGCTTGGA+AGG + tig0023324:54365-54384 Msa1407770:five_prime_UTR 40.0%
CAGCATCTAATCAAGAAAGC+TGG + tig0023324:54418-54437 Msa1407770:five_prime_UTR 40.0%
CATCTAATCAAGAAAGCTGG+TGG + tig0023324:54421-54440 Msa1407770:five_prime_UTR 40.0%
GCATAAAATGATCAATGCGG+AGG - tig0023324:54464-54483 None:intergenic 40.0%
TCATGCAATATGCCAAGAGA+TGG + tig0023324:54541-54560 Msa1407770:five_prime_UTR 40.0%
TCTAACTCCTCATACATTCC+TGG - tig0023324:54570-54589 None:intergenic 40.0%
GGAATGTATGAGGAGTTAGA+AGG + tig0023324:54570-54589 Msa1407770:five_prime_UTR 40.0%
TTAGAAGGGCTTAGAGGATA+TGG + tig0023324:54585-54604 Msa1407770:five_prime_UTR 40.0%
TAGAAGGGCTTAGAGGATAT+GGG + tig0023324:54586-54605 Msa1407770:five_prime_UTR 40.0%
CTTGTTGCAGATGCTAAAGA+TGG + tig0023324:54748-54767 Msa1407770:exon 40.0%
TCAGACCTTGAAAAGAGAGT+TGG + tig0023324:54803-54822 Msa1407770:CDS 40.0%
! CCATACCAACTCTCTTTTCA+AGG - tig0023324:54811-54830 None:intergenic 40.0%
!! CCTTGAAAAGAGAGTTGGTA+TGG + tig0023324:54808-54827 Msa1407770:CDS 40.0%
! TATGGTGTTAGAAGATGCTG+AGG + tig0023324:54826-54845 Msa1407770:CDS 40.0%
GGAATTGGCATTAATGCTGA+AGG + tig0023324:54941-54960 Msa1407770:intron 40.0%
!! TGGTCATTTTCATAAGCCTC+CGG - tig0023324:54999-55018 None:intergenic 40.0%
! TCTTGCATAGTGCAGTTTTC+GGG - tig0023324:55090-55109 None:intergenic 40.0%
! GTCTTGCATAGTGCAGTTTT+CGG - tig0023324:55091-55110 None:intergenic 40.0%
ATGCAACTGCAACAAAAGTC+CGG + tig0023324:55165-55184 Msa1407770:CDS 40.0%
CATACTCGATCATTTCACGA+TGG + tig0023324:55294-55313 Msa1407770:CDS 40.0%
TAAAAGGCTGACCTTTAGGT+AGG - tig0023324:55340-55359 None:intergenic 40.0%
GACTAAGTTCCAAAGATCCA+GGG - tig0023324:55592-55611 None:intergenic 40.0%
AGACTAAGTTCCAAAGATCC+AGG - tig0023324:55593-55612 None:intergenic 40.0%
GGTATTTAAGCAGATGCTAG+TGG - tig0023324:55709-55728 None:intergenic 40.0%
GAAAACATCTGACCCCAAAT+TGG - tig0023324:55767-55786 None:intergenic 40.0%
! AGTTTTCCATCTGCATGGAA+GGG - tig0023324:55843-55862 None:intergenic 40.0%
! TAGTTTTCCATCTGCATGGA+AGG - tig0023324:55844-55863 None:intergenic 40.0%
!!! TCACTAGTTTTCCATCTGCA+TGG - tig0023324:55848-55867 None:intergenic 40.0%
!! ATGACACATGATTTTGGGGA+GGG - tig0023324:56007-56026 None:intergenic 40.0%
!! TATGACACATGATTTTGGGG+AGG - tig0023324:56008-56027 None:intergenic 40.0%
! ACATGGCATCTAAGATAGTG+TGG + tig0023324:56306-56325 Msa1407770:intron 40.0%
! TGCGTTGTATACTTTGGTTC+AGG - tig0023324:56423-56442 None:intergenic 40.0%
TGAACCAAAGTATACAACGC+AGG + tig0023324:56422-56441 Msa1407770:intron 40.0%
AGAACCTGCGTTGTATACTT+TGG - tig0023324:56429-56448 None:intergenic 40.0%
AAGTGGAATCAACTCTCAAC+AGG + tig0023324:56503-56522 Msa1407770:CDS 40.0%
CGAGAATGTAAAGTCCAACA+TGG + tig0023324:56578-56597 Msa1407770:CDS 40.0%
AACCAAAGAATCTATCAGGC+TGG + tig0023324:56664-56683 Msa1407770:CDS 40.0%
TCCTTCCATGTAAAACCAAC+AGG + tig0023324:56714-56733 Msa1407770:CDS 40.0%
!!! TCCTGTTGGTTTTACATGGA+AGG - tig0023324:56718-56737 None:intergenic 40.0%
TTCCATGTAAAACCAACAGG+AGG + tig0023324:56717-56736 Msa1407770:CDS 40.0%
GAGCTAATTGCTGCAAGCTT+GGG - tig0023324:52993-53012 None:intergenic 45.0%
TGAGCTAATTGCTGCAAGCT+TGG - tig0023324:52994-53013 None:intergenic 45.0%
! ACTTTCAGGGTCTTGTGCTT+GGG - tig0023324:53045-53064 None:intergenic 45.0%
! GTTCTGTCAGACTGACTTTC+AGG - tig0023324:53059-53078 None:intergenic 45.0%
AAAGTCTGTTGGGATTCGAG+GGG - tig0023324:53328-53347 None:intergenic 45.0%
GAAAAGTCTGTTGGGATTCG+AGG - tig0023324:53330-53349 None:intergenic 45.0%
ACCCTGCTGATGATACTGTT+TGG - tig0023324:53464-53483 None:intergenic 45.0%
CACACACACACAAACACACT+AGG - tig0023324:53615-53634 None:intergenic 45.0%
TCTATCCAAAAGAGGTGGTG+GGG - tig0023324:53674-53693 None:intergenic 45.0%
CTCTATCCAAAAGAGGTGGT+GGG - tig0023324:53675-53694 None:intergenic 45.0%
TCTCTATCCAAAAGAGGTGG+TGG - tig0023324:53676-53695 None:intergenic 45.0%
GGGAAAATTTCCCTCAAGAG+AGG + tig0023324:53702-53721 Msa1407770:intron 45.0%
TCTCTGTCTACCTCTCTTGA+GGG - tig0023324:53715-53734 None:intergenic 45.0%
TTCTCTGTCTACCTCTCTTG+AGG - tig0023324:53716-53735 None:intergenic 45.0%
CAAAATGCGGCTACATAGGA+CGG + tig0023324:54019-54038 Msa1407770:five_prime_UTR 45.0%
CCTCCCTATAGACTTCAACT+CGG + tig0023324:54146-54165 Msa1407770:five_prime_UTR 45.0%
TGTGAGGAAAGCTTCAGTCT+TGG - tig0023324:54247-54266 None:intergenic 45.0%
TAAAAATGAGCCCGCAAGTG+AGG + tig0023324:54392-54411 Msa1407770:five_prime_UTR 45.0%
AAGCTGGTGGTTCGATGATT+TGG + tig0023324:54434-54453 Msa1407770:five_prime_UTR 45.0%
AAAATGATCAATGCGGAGGG+TGG - tig0023324:54460-54479 None:intergenic 45.0%
GGAACCAAACCAGAGATCAT+TGG + tig0023324:54510-54529 Msa1407770:five_prime_UTR 45.0%
ATGGCTACCAGGAATGTATG+AGG + tig0023324:54560-54579 Msa1407770:five_prime_UTR 45.0%
!! AGAGAGCACTAGACATAGCA+AGG + tig0023324:54656-54675 Msa1407770:five_prime_UTR 45.0%
!!! GTGCAGTTTTCGGGTGAATT+AGG - tig0023324:55081-55100 None:intergenic 45.0%
TGCAACTGCAACAAAAGTCC+GGG + tig0023324:55166-55185 Msa1407770:CDS 45.0%
GATCTGTTGCAACTTCAGCT+AGG - tig0023324:55224-55243 None:intergenic 45.0%
AGCCATTGACACCTACCTAA+AGG + tig0023324:55326-55345 Msa1407770:CDS 45.0%
GACCTTTAGGTAGGTGTCAA+TGG - tig0023324:55331-55350 None:intergenic 45.0%
TTGGTCAGTTAGGGAAGGAT+GGG - tig0023324:55456-55475 None:intergenic 45.0%
TCTACGGTCTTGGTCAGTTA+GGG - tig0023324:55465-55484 None:intergenic 45.0%
TTCTACGGTCTTGGTCAGTT+AGG - tig0023324:55466-55485 None:intergenic 45.0%
ACCTGGACAACTGTTCTTAG+AGG - tig0023324:55563-55582 None:intergenic 45.0%
AAGATCCAGGGAACTTTACC+TGG - tig0023324:55580-55599 None:intergenic 45.0%
GGTAAAGTTCCCTGGATCTT+TGG + tig0023324:55580-55599 Msa1407770:intron 45.0%
AGAAATCCCTTCCATGCAGA+TGG + tig0023324:55834-55853 Msa1407770:intron 45.0%
AGCAATGCAAGAGAAGGAAC+CGG + tig0023324:56476-56495 Msa1407770:CDS 45.0%
! GAGTTGATTCCACTTTGTGC+CGG - tig0023324:56498-56517 None:intergenic 45.0%
GTTCTGCAGTTCTACCATGT+TGG - tig0023324:56595-56614 None:intergenic 45.0%
CTCCAGCCTGATAGATTCTT+TGG - tig0023324:56669-56688 None:intergenic 45.0%
CAGGCTGGAGTTTAAATTGG+CGG + tig0023324:56679-56698 Msa1407770:CDS 45.0%
!!! CACCTCCTGTTGGTTTTACA+TGG - tig0023324:56722-56741 None:intergenic 45.0%
! TCTCTAGTTTCACCTCCTGT+TGG - tig0023324:56732-56751 None:intergenic 45.0%
ACAGGAGGTGAAACTAGAGA+TGG + tig0023324:56732-56751 Msa1407770:CDS 45.0%
!! TTAGATATAATATAAAATTT+AGG - tig0023324:56890-56909 None:intergenic 5.0%
! GACTTTCAGGGTCTTGTGCT+TGG - tig0023324:53046-53065 None:intergenic 50.0%
! CCGAGTTGAAGTCTATAGGG+AGG - tig0023324:54149-54168 None:intergenic 50.0%
AGATGCTGCATCCTCACTTG+CGG - tig0023324:54406-54425 None:intergenic 50.0%
TATGCCAAGAGATGGCTACC+AGG + tig0023324:54549-54568 Msa1407770:five_prime_UTR 50.0%
CATTCCTGGTAGCCATCTCT+TGG - tig0023324:54556-54575 None:intergenic 50.0%
GAGGAGTTAGAAGGGCTTAG+AGG + tig0023324:54579-54598 Msa1407770:five_prime_UTR 50.0%
CATGGGACAGCATCTTGTTG+AGG - tig0023324:54722-54741 None:intergenic 50.0%
CTTGGTCAGTTAGGGAAGGA+TGG - tig0023324:55457-55476 None:intergenic 50.0%
TGCAGAGTCTTCTACGGTCT+TGG - tig0023324:55475-55494 None:intergenic 50.0%
! GCCTCTAAGAACAGTTGTCC+AGG + tig0023324:55559-55578 Msa1407770:CDS 50.0%
GTTGTCCAGGTAAAGTTCCC+TGG + tig0023324:55572-55591 Msa1407770:intron 50.0%
GAGAGCAGCAATGCAAGAGA+AGG + tig0023324:56470-56489 Msa1407770:CDS 50.0%
!! CGAGTGTGGTTTGGTGATGT+GGG - tig0023324:56768-56787 None:intergenic 50.0%
!! ACGAGTGTGGTTTGGTGATG+TGG - tig0023324:56769-56788 None:intergenic 50.0%
GATCTACGACGAGTGTGGTT+TGG - tig0023324:56777-56796 None:intergenic 50.0%
!! TCCAAAAGAGGTGGTGGGGT+GGG - tig0023324:53670-53689 None:intergenic 55.0%
!! ATCCAAAAGAGGTGGTGGGG+TGG - tig0023324:53671-53690 None:intergenic 55.0%
ATGCGGCTACATAGGACGGT+CGG + tig0023324:54023-54042 Msa1407770:five_prime_UTR 55.0%
GATGCTGCATCCTCACTTGC+GGG - tig0023324:54405-54424 None:intergenic 55.0%
GGGCTTAGAGGATATGGGCA+TGG + tig0023324:54591-54610 Msa1407770:five_prime_UTR 55.0%
GGGACAGCATCTTGTTGAGG+AGG - tig0023324:54719-54738 None:intergenic 55.0%
CGGTCTTGGTCAGTTAGGGA+AGG - tig0023324:55461-55480 None:intergenic 55.0%
GAGAAGGAACCGGCACAAAG+TGG + tig0023324:56486-56505 Msa1407770:CDS 55.0%
GTAGATCAGCCCCGAGAAGA+AGG + tig0023324:56790-56809 Msa1407770:CDS 55.0%
ACACGGAGAGCCTTCTTCTC+GGG - tig0023324:56803-56822 None:intergenic 55.0%
GACACGGAGAGCCTTCTTCT+CGG - tig0023324:56804-56823 None:intergenic 55.0%
! TCCCACCCCACCACCTCTTT+TGG + tig0023324:53666-53685 Msa1407770:intron 60.0%
GGGCTGATCTACGACGAGTG+TGG - tig0023324:56782-56801 None:intergenic 60.0%
CACGGAGAGCCTTCTTCTCG+GGG - tig0023324:56802-56821 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
tig0023324 gene 52862 56920 52862 ID=Msa1407770;Name=Msa1407770
tig0023324 mRNA 52862 56920 52862 ID=Msa1407770-mRNA-1;Parent=Msa1407770;Name=Msa1407770-mRNA-1;_AED=0.07;_eAED=0.07;_QI=1120|1|1|1|1|0.83|6|93|310
tig0023324 exon 52862 53159 52862 ID=Msa1407770-mRNA-1:exon:25253;Parent=Msa1407770-mRNA-1
tig0023324 exon 53317 53386 53317 ID=Msa1407770-mRNA-1:exon:25254;Parent=Msa1407770-mRNA-1
tig0023324 exon 54006 54903 54006 ID=Msa1407770-mRNA-1:exon:25255;Parent=Msa1407770-mRNA-1
tig0023324 exon 55074 55347 55074 ID=Msa1407770-mRNA-1:exon:25256;Parent=Msa1407770-mRNA-1
tig0023324 exon 55452 55580 55452 ID=Msa1407770-mRNA-1:exon:25257;Parent=Msa1407770-mRNA-1
tig0023324 exon 56444 56920 56444 ID=Msa1407770-mRNA-1:exon:25258;Parent=Msa1407770-mRNA-1
tig0023324 five_prime_UTR 52862 53159 52862 ID=Msa1407770-mRNA-1:five_prime_utr;Parent=Msa1407770-mRNA-1
tig0023324 five_prime_UTR 53317 53386 53317 ID=Msa1407770-mRNA-1:five_prime_utr;Parent=Msa1407770-mRNA-1
tig0023324 five_prime_UTR 54006 54757 54006 ID=Msa1407770-mRNA-1:five_prime_utr;Parent=Msa1407770-mRNA-1
tig0023324 CDS 54758 54903 54758 ID=Msa1407770-mRNA-1:cds;Parent=Msa1407770-mRNA-1
tig0023324 CDS 55074 55347 55074 ID=Msa1407770-mRNA-1:cds;Parent=Msa1407770-mRNA-1
tig0023324 CDS 55452 55580 55452 ID=Msa1407770-mRNA-1:cds;Parent=Msa1407770-mRNA-1
tig0023324 CDS 56444 56827 56444 ID=Msa1407770-mRNA-1:cds;Parent=Msa1407770-mRNA-1
tig0023324 three_prime_UTR 56828 56920 56828 ID=Msa1407770-mRNA-1:three_prime_utr;Parent=Msa1407770-mRNA-1
Gene Sequence

>Msa1407770

ATGCTAAAGATGGCAATGATGTATTCTGTATCATCAGCTCTCATTTCAGACCTTGAAAAGAGAGTTGGTATGGTGTTAGAAGATGCTGAGGTGAATGATCTCTTAATTCCTAGATATCAAAATGGAGGTCAAGGAAAAATGGTCAACATGCCTAATTCACCCGAAAACTGCACTATGCAAGACATAGATGTAGTACAACGGATTGTTGAATACTTCTTGATGCATGAAAAACAGCAAATGCAACTGCAACAAAAGTCCGGGAAATTCAATATTAGTAGGCTTTTGGACAATTACCTAGCTGAAGTTGCAACAGATCCAAATCTTTCAATTACAATGTTTCAAGTCTTAGCTGAATTACTGCCAGAACATACTCGATCATTTCACGATGGTCTATATAGAGCCATTGACACCTACCTAAAGACCCATCCTTCCCTAACTGACCAAGACCGTAGAAGACTCTGCAAAATAATGAACTGTGAAAAACTCTCACTTGATGCATGCATACATGCTGCACAAAATGATAGATTGCCTCTAAGAACAGTTGTCCAGGTTCTATTTGCAGAGCAAGTAAAGATGAGAGCAGCAATGCAAGAGAAGGAACCGGCACAAAGTGGAATCAACTCTCAACAGGATGTAAACCAGACATCTGAAACTATAGACATAAAAACACTCAAAGCAGAACTCGAGAATGTAAAGTCCAACATGGTAGAACTGCAGAACGACTACTTTGAGTTGCAAGAAGAGTATAAAAAGTTAACTAGCAACGACAAACCAAAGAATCTATCAGGCTGGAGTTTAAATTGGCGGAAGATCAAGAAATCCTTCCATGTAAAACCAACAGGAGGTGAAACTAGAGATGGACAAGATATGCCCACATCACCAAACCACACTCGTCGTAGATCAGCCCCGAGAAGAAGGCTCTCCGTGTCTTAA
Protein sequence

>Msa1407770

MLKMAMMYSVSSALISDLEKRVGMVLEDAEVNDLLIPRYQNGGQGKMVNMPNSPENCTMQDIDVVQRIVEYFLMHEKQQMQLQQKSGKFNISRLLDNYLAEVATDPNLSITMFQVLAELLPEHTRSFHDGLYRAIDTYLKTHPSLTDQDRRRLCKIMNCEKLSLDACIHAAQNDRLPLRTVVQVLFAEQVKMRAAMQEKEPAQSGINSQQDVNQTSETIDIKTLKAELENVKSNMVELQNDYFELQEEYKKLTSNDKPKNLSGWSLNWRKIKKSFHVKPTGGETRDGQDMPTSPNHTRRRSAPRRRLSVS*