AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene000634


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene000634.t1 MTR_3g053920 59.375 128 35 2 56 166 153 280 2.50e-40 139
MS.gene000634.t1 MTR_8g463110 79.000 100 21 0 129 228 21 120 2.58e-34 129
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 69 sgRNAs with CRISPR-Local

Find 77 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTACCGATACGTTTCAAATT+AGG 0.227389 5.1:-19239606 None:intergenic
CTGACGACGGTGTCCGTAAC+TGG 0.261198 5.1:+19239568 MS.gene000634:CDS
GAATTTGCAGCAGTAGTAAA+AGG 0.292724 5.1:-19239960 None:intergenic
GATGAGGGAAATACAGTAAT+AGG 0.305593 5.1:+19240071 MS.gene000634:CDS
CACCTTCAGCAGTTACATTT+CGG 0.325416 5.1:-19239713 None:intergenic
CTGGTGCTTGGACTGTCTGC+TGG 0.392114 5.1:+19239930 MS.gene000634:CDS
CCTACTTGGCCTCTGGTGCT+TGG 0.396214 5.1:+19239918 MS.gene000634:CDS
TAACTTTAAATATGAAAAGC+TGG 0.406564 5.1:+19239473 MS.gene000634:CDS
CGCGGCACAGCTACGGGAAT+AGG 0.408134 5.1:-19239657 None:intergenic
GACACGCCACTCTTGTTTCA+AGG 0.413036 5.1:-19239421 None:intergenic
GCCAAAGCAAAACAAGGTTC+TGG 0.414080 5.1:+19239825 MS.gene000634:CDS
GGTCCAATCTGCTTCCTACT+TGG 0.416973 5.1:+19239904 MS.gene000634:CDS
CTCTTACCGTCCTCTTCAAC+AGG 0.419316 5.1:+19239873 MS.gene000634:CDS
GCAAATTCTCAAGAGGAAAT+CGG 0.445460 5.1:+19239975 MS.gene000634:CDS
TCTGCTTCCTACTTGGCCTC+TGG 0.449798 5.1:+19239911 MS.gene000634:CDS
GCAGCAGTAGTAAAAGGAAA+TGG 0.452091 5.1:-19239954 None:intergenic
TCGGTACTGCTGCAACCAAC+AGG 0.460210 5.1:+19239622 MS.gene000634:CDS
GTTGTTGCTGCTGCCTCTGT+TGG 0.468674 5.1:+19239690 MS.gene000634:CDS
GGTTCTGGTGCGAACTCTCC+AGG 0.468743 5.1:+19239840 MS.gene000634:CDS
CAGCAGCAACAACATTTGTA+TGG 0.473033 5.1:-19239680 None:intergenic
TGAGATAAGCACCCCAGTTA+CGG 0.479656 5.1:-19239581 None:intergenic
GCAGACAGTCCAAGCACCAG+AGG 0.486586 5.1:-19239927 None:intergenic
TGTAACTGCTGAAGGTGTAC+TGG 0.490437 5.1:+19239719 MS.gene000634:CDS
TCCTCTTCAACAGGTAACTC+AGG 0.492293 5.1:+19239882 MS.gene000634:CDS
TGCAACCAACAGGTGGATAC+AGG 0.492385 5.1:+19239632 MS.gene000634:CDS
TGTATGGCGCGGCACAGCTA+CGG 0.503703 5.1:-19239664 None:intergenic
GTGTGGTGTGCTCGGACATA+CGG 0.504521 5.1:+19239512 MS.gene000634:CDS
CCAAGCACCAGAGGCCAAGT+AGG 0.508320 5.1:-19239918 None:intergenic
AACTTTAAATATGAAAAGCT+GGG 0.509761 5.1:+19239474 MS.gene000634:CDS
TAGGAACAAATGTTAGTCAT+GGG 0.515296 5.1:+19240090 MS.gene000634:CDS
ACATACGGACAAGGTGTGTC+CGG 0.515722 5.1:+19239527 MS.gene000634:CDS
ACCATCCTGTCATTGAAGCT+TGG 0.516139 5.1:-19239744 None:intergenic
GGTAAGAGATGTACCATGCC+TGG 0.518626 5.1:-19239858 None:intergenic
AGGCCAAGTAGGAAGCAGAT+TGG 0.522212 5.1:-19239907 None:intergenic
TGACGACGGTGTCCGTAACT+GGG 0.523857 5.1:+19239569 MS.gene000634:CDS
GAAGAAAATAAAGAGGATGC+TGG 0.533193 5.1:+19240007 MS.gene000634:CDS
TGGTTCCAAGCTTCAATGAC+AGG 0.533487 5.1:+19239739 MS.gene000634:CDS
GTATGGCGCGGCACAGCTAC+GGG 0.534441 5.1:-19239663 None:intergenic
GCTTTCAATTCAAACAGCTC+CGG 0.535106 5.1:-19239546 None:intergenic
TGTCACAGGTGTGGTGTGCT+CGG 0.535855 5.1:+19239504 MS.gene000634:CDS
ATAGGAACAAATGTTAGTCA+TGG 0.543713 5.1:+19240089 MS.gene000634:CDS
ATGAATTGTCACCATTCTGA+AGG 0.545818 5.1:-19240031 None:intergenic
CCCTGAGTTACCTGTTGAAG+AGG 0.550310 5.1:-19239883 None:intergenic
AAGCCTAATTTGAAACGTAT+CGG 0.565791 5.1:+19239603 MS.gene000634:CDS
TACTGCTGCAAATTCTCAAG+AGG 0.568886 5.1:+19239968 MS.gene000634:CDS
CTGAATTGAAGAAAATAAAG+AGG 0.569177 5.1:+19240000 MS.gene000634:CDS
ACACGCCACTCTTGTTTCAA+GGG 0.575839 5.1:-19239420 None:intergenic
CAAAAGTACCATTGTTAGCA+CGG 0.580700 5.1:-19239443 None:intergenic
TGGTGCGAACTCTCCAGGCA+TGG 0.581722 5.1:+19239845 MS.gene000634:CDS
AGGATGCTGGCCCTTCAGAA+TGG 0.591615 5.1:+19240020 MS.gene000634:CDS
AGGATCCTGTATCCACCTGT+TGG 0.607557 5.1:-19239637 None:intergenic
TCCAAGCTTCAATGACAGGA+TGG 0.614971 5.1:+19239743 MS.gene000634:CDS
TTTGCTGCTGATGAGGATGA+GGG 0.616001 5.1:+19240056 MS.gene000634:CDS
AGGAACAAATGTTAGTCATG+GGG 0.621613 5.1:+19240091 MS.gene000634:CDS
GGCCGAAATGTAACTGCTGA+AGG 0.622032 5.1:+19239711 MS.gene000634:CDS
GGAACAAATGTTAGTCATGG+GGG 0.626566 5.1:+19240092 MS.gene000634:CDS
TGGCGTGTCCGTGCTAACAA+TGG 0.633755 5.1:+19239435 MS.gene000634:CDS
GACGACGGTGTCCGTAACTG+GGG 0.635519 5.1:+19239570 MS.gene000634:CDS
TGAATTGTCACCATTCTGAA+GGG 0.638224 5.1:-19240030 None:intergenic
CCTCTTCAACAGGTAACTCA+GGG 0.640759 5.1:+19239883 MS.gene000634:CDS
GCAACAACATTTGTATGGCG+CGG 0.643692 5.1:-19239675 None:intergenic
GTACTGCTGCAACCAACAGG+TGG 0.653037 5.1:+19239625 MS.gene000634:CDS
CTGTTCCCTTGAAACAAGAG+TGG 0.655372 5.1:+19239415 MS.gene000634:CDS
TAGGCTGCCAAAGCAAAACA+AGG 0.657259 5.1:+19239819 MS.gene000634:intron
GAACAAATGTTAGTCATGGG+GGG 0.666834 5.1:+19240093 MS.gene000634:CDS
GAGTTACCTGTTGAAGAGGA+CGG 0.671052 5.1:-19239879 None:intergenic
TGTGCTCGGACATACGGACA+AGG 0.675926 5.1:+19239518 MS.gene000634:CDS
AGTTACATTTCGGCCAACAG+AGG 0.697810 5.1:-19239703 None:intergenic
TTTGAATTGAAAGCTGACGA+CGG 0.717213 5.1:+19239555 MS.gene000634:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTAAATATGAAAAGCT+GGG + chr5.1:19239474-19239493 MS.gene000634:CDS 20.0%
!! TAACTTTAAATATGAAAAGC+TGG + chr5.1:19239473-19239492 MS.gene000634:CDS 20.0%
! CTGAATTGAAGAAAATAAAG+AGG + chr5.1:19240000-19240019 MS.gene000634:CDS 25.0%
ATAGGAACAAATGTTAGTCA+TGG + chr5.1:19240089-19240108 MS.gene000634:CDS 30.0%
TAGGAACAAATGTTAGTCAT+GGG + chr5.1:19240090-19240109 MS.gene000634:CDS 30.0%
! AAGCCTAATTTGAAACGTAT+CGG + chr5.1:19239603-19239622 MS.gene000634:CDS 30.0%
AGGAACAAATGTTAGTCATG+GGG + chr5.1:19240091-19240110 MS.gene000634:CDS 35.0%
ATCACTGCAATCAAACATGA+TGG + chr5.1:19239783-19239802 MS.gene000634:intron 35.0%
ATGAATTGTCACCATTCTGA+AGG - chr5.1:19240034-19240053 None:intergenic 35.0%
CATGTTTGATTGCAGTGATT+TGG - chr5.1:19239782-19239801 None:intergenic 35.0%
GAAGAAAATAAAGAGGATGC+TGG + chr5.1:19240007-19240026 MS.gene000634:CDS 35.0%
GAATTTGCAGCAGTAGTAAA+AGG - chr5.1:19239963-19239982 None:intergenic 35.0%
GATGAGGGAAATACAGTAAT+AGG + chr5.1:19240071-19240090 MS.gene000634:CDS 35.0%
GCAAATTCTCAAGAGGAAAT+CGG + chr5.1:19239975-19239994 MS.gene000634:CDS 35.0%
TGAATTGTCACCATTCTGAA+GGG - chr5.1:19240033-19240052 None:intergenic 35.0%
TTTGAATTGAAAGCTGACGA+CGG + chr5.1:19239555-19239574 MS.gene000634:CDS 35.0%
! GTACCGATACGTTTCAAATT+AGG - chr5.1:19239609-19239628 None:intergenic 35.0%
!! CAAAAGTACCATTGTTAGCA+CGG - chr5.1:19239446-19239465 None:intergenic 35.0%
CACCTTCAGCAGTTACATTT+CGG - chr5.1:19239716-19239735 None:intergenic 40.0%
CAGCAGCAACAACATTTGTA+TGG - chr5.1:19239683-19239702 None:intergenic 40.0%
GAACAAATGTTAGTCATGGG+GGG + chr5.1:19240093-19240112 MS.gene000634:CDS 40.0%
GCAGCAGTAGTAAAAGGAAA+TGG - chr5.1:19239957-19239976 None:intergenic 40.0%
GCTTTCAATTCAAACAGCTC+CGG - chr5.1:19239549-19239568 None:intergenic 40.0%
GGAACAAATGTTAGTCATGG+GGG + chr5.1:19240092-19240111 MS.gene000634:CDS 40.0%
TACTGCTGCAAATTCTCAAG+AGG + chr5.1:19239968-19239987 MS.gene000634:CDS 40.0%
! TTCATCTTTTGCTGCTGATG+AGG + chr5.1:19240049-19240068 MS.gene000634:CDS 40.0%
!!! ACCAGAACCTTGTTTTGCTT+TGG - chr5.1:19239829-19239848 None:intergenic 40.0%
ACCATCCTGTCATTGAAGCT+TGG - chr5.1:19239747-19239766 None:intergenic 45.0%
AGTTACATTTCGGCCAACAG+AGG - chr5.1:19239706-19239725 None:intergenic 45.0%
CCTCTTCAACAGGTAACTCA+GGG + chr5.1:19239883-19239902 MS.gene000634:CDS 45.0%
CTGTTCCCTTGAAACAAGAG+TGG + chr5.1:19239415-19239434 MS.gene000634:CDS 45.0%
GAGTTACCTGTTGAAGAGGA+CGG - chr5.1:19239882-19239901 None:intergenic 45.0%
GCAACAACATTTGTATGGCG+CGG - chr5.1:19239678-19239697 None:intergenic 45.0%
GCCAAAGCAAAACAAGGTTC+TGG + chr5.1:19239825-19239844 MS.gene000634:CDS 45.0%
TAGGCTGCCAAAGCAAAACA+AGG + chr5.1:19239819-19239838 MS.gene000634:intron 45.0%
TCCAAGCTTCAATGACAGGA+TGG + chr5.1:19239743-19239762 MS.gene000634:CDS 45.0%
TCCTCTTCAACAGGTAACTC+AGG + chr5.1:19239882-19239901 MS.gene000634:CDS 45.0%
TGAGATAAGCACCCCAGTTA+CGG - chr5.1:19239584-19239603 None:intergenic 45.0%
TGGTTCCAAGCTTCAATGAC+AGG + chr5.1:19239739-19239758 MS.gene000634:CDS 45.0%
TTTGCTGCTGATGAGGATGA+GGG + chr5.1:19240056-19240075 MS.gene000634:CDS 45.0%
! ACACGCCACTCTTGTTTCAA+GGG - chr5.1:19239423-19239442 None:intergenic 45.0%
! TGATGGTCTTGCTGCTCAAT+AGG + chr5.1:19239800-19239819 MS.gene000634:intron 45.0%
! TGTAACTGCTGAAGGTGTAC+TGG + chr5.1:19239719-19239738 MS.gene000634:CDS 45.0%
! TTTTGCTGCTGATGAGGATG+AGG + chr5.1:19240055-19240074 MS.gene000634:CDS 45.0%
!!! AGCTGGGTGTTTTTTGTCAC+AGG + chr5.1:19239490-19239509 MS.gene000634:CDS 45.0%
!!! GGTGTTTTTTGTCACAGGTG+TGG + chr5.1:19239495-19239514 MS.gene000634:CDS 45.0%
ACATACGGACAAGGTGTGTC+CGG + chr5.1:19239527-19239546 MS.gene000634:CDS 50.0%
AGGATCCTGTATCCACCTGT+TGG - chr5.1:19239640-19239659 None:intergenic 50.0%
AGGCCAAGTAGGAAGCAGAT+TGG - chr5.1:19239910-19239929 None:intergenic 50.0%
CCCTGAGTTACCTGTTGAAG+AGG - chr5.1:19239886-19239905 None:intergenic 50.0%
CTCTTACCGTCCTCTTCAAC+AGG + chr5.1:19239873-19239892 MS.gene000634:CDS 50.0%
GGCCGAAATGTAACTGCTGA+AGG + chr5.1:19239711-19239730 MS.gene000634:CDS 50.0%
GGTAAGAGATGTACCATGCC+TGG - chr5.1:19239861-19239880 None:intergenic 50.0%
GGTCCAATCTGCTTCCTACT+TGG + chr5.1:19239904-19239923 MS.gene000634:CDS 50.0%
TGCAACCAACAGGTGGATAC+AGG + chr5.1:19239632-19239651 MS.gene000634:CDS 50.0%
! GACACGCCACTCTTGTTTCA+AGG - chr5.1:19239424-19239443 None:intergenic 50.0%
AGGATGCTGGCCCTTCAGAA+TGG + chr5.1:19240020-19240039 MS.gene000634:CDS 55.0%
GTACTGCTGCAACCAACAGG+TGG + chr5.1:19239625-19239644 MS.gene000634:CDS 55.0%
GTGTGGTGTGCTCGGACATA+CGG + chr5.1:19239512-19239531 MS.gene000634:CDS 55.0%
GTTGTTGCTGCTGCCTCTGT+TGG + chr5.1:19239690-19239709 MS.gene000634:CDS 55.0%
TCGGTACTGCTGCAACCAAC+AGG + chr5.1:19239622-19239641 MS.gene000634:CDS 55.0%
TCTGCTTCCTACTTGGCCTC+TGG + chr5.1:19239911-19239930 MS.gene000634:CDS 55.0%
TGACGACGGTGTCCGTAACT+GGG + chr5.1:19239569-19239588 MS.gene000634:CDS 55.0%
TGGCGTGTCCGTGCTAACAA+TGG + chr5.1:19239435-19239454 MS.gene000634:CDS 55.0%
TGTCACAGGTGTGGTGTGCT+CGG + chr5.1:19239504-19239523 MS.gene000634:CDS 55.0%
TGTGCTCGGACATACGGACA+AGG + chr5.1:19239518-19239537 MS.gene000634:CDS 55.0%
CCAAGCACCAGAGGCCAAGT+AGG - chr5.1:19239921-19239940 None:intergenic 60.0%
CCTACTTGGCCTCTGGTGCT+TGG + chr5.1:19239918-19239937 MS.gene000634:CDS 60.0%
CTGACGACGGTGTCCGTAAC+TGG + chr5.1:19239568-19239587 MS.gene000634:CDS 60.0%
CTGGTGCTTGGACTGTCTGC+TGG + chr5.1:19239930-19239949 MS.gene000634:CDS 60.0%
GACGACGGTGTCCGTAACTG+GGG + chr5.1:19239570-19239589 MS.gene000634:CDS 60.0%
GCAGACAGTCCAAGCACCAG+AGG - chr5.1:19239930-19239949 None:intergenic 60.0%
TGGTGCGAACTCTCCAGGCA+TGG + chr5.1:19239845-19239864 MS.gene000634:CDS 60.0%
TGTATGGCGCGGCACAGCTA+CGG - chr5.1:19239667-19239686 None:intergenic 60.0%
! GGTTCTGGTGCGAACTCTCC+AGG + chr5.1:19239840-19239859 MS.gene000634:CDS 60.0%
CGCGGCACAGCTACGGGAAT+AGG - chr5.1:19239660-19239679 None:intergenic 65.0%
GTATGGCGCGGCACAGCTAC+GGG - chr5.1:19239666-19239685 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 19239387 19240127 19239387 ID=MS.gene000634
chr5.1 mRNA 19239387 19240127 19239387 ID=MS.gene000634.t1;Parent=MS.gene000634
chr5.1 exon 19239387 19239770 19239387 ID=MS.gene000634.t1.exon1;Parent=MS.gene000634.t1
chr5.1 CDS 19239387 19239770 19239387 ID=cds.MS.gene000634.t1;Parent=MS.gene000634.t1
chr5.1 exon 19239822 19240127 19239822 ID=MS.gene000634.t1.exon2;Parent=MS.gene000634.t1
chr5.1 CDS 19239822 19240127 19239822 ID=cds.MS.gene000634.t1;Parent=MS.gene000634.t1
Gene Sequence

>MS.gene000634

ATGCGTATCTGTGTTGCAATTGATGTGACTGTTCCCTTGAAACAAGAGTGGCGTGTCCGTGCTAACAATGGTACTTTTGTTACTGTTAACTTTAAATATGAAAAGCTGGGTGTTTTTTGTCACAGGTGTGGTGTGCTCGGACATACGGACAAGGTGTGTCCGGAGCTGTTTGAATTGAAAGCTGACGACGGTGTCCGTAACTGGGGTGCTTATCTCAAGCCTAATTTGAAACGTATCGGTACTGCTGCAACCAACAGGTGGATACAGGATCCTATTCCCGTAGCTGTGCCGCGCCATACAAATGTTGTTGCTGCTGCCTCTGTTGGCCGAAATGTAACTGCTGAAGGTGTACTGGTTCCAAGCTTCAATGACAGGATGGTAGCTGTACAGAACCAAATCACTGCAATCAAACATGATGGTCTTGCTGCTCAATAGGCTGCCAAAGCAAAACAAGGTTCTGGTGCGAACTCTCCAGGCATGGTACATCTCTTACCGTCCTCTTCAACAGGTAACTCAGGGTCCAATCTGCTTCCTACTTGGCCTCTGGTGCTTGGACTGTCTGCTGGTCCATTTCCTTTTACTACTGCTGCAAATTCTCAAGAGGAAATCGGCTCTGAATTGAAGAAAATAAAGAGGATGCTGGCCCTTCAGAATGGTGACAATTCATCTTTTGCTGCTGATGAGGATGAGGGAAATACAGTAATAGGAACAAATGTTAGTCATGGGGGGATGATGATATGA

Protein sequence

>MS.gene000634.t1

MRICVAIDVTVPLKQEWRVRANNGTFVTVNFKYEKLGVFCHRCGVLGHTDKVCPELFELKADDGVRNWGAYLKPNLKRIGTAATNRWIQDPIPVAVPRHTNVVAAASVGRNVTAEGVLVPSFNDRMVAAAKAKQGSGANSPGMVHLLPSSSTGNSGSNLLPTWPLVLGLSAGPFPFTTAANSQEEIGSELKKIKRMLALQNGDNSSFAADEDEGNTVIGTNVSHGGMMI