AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene96109

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96109.t1 MTR_3g053920 57.971 345 79 3 28 372 1 279 6.38e-131 377
MS.gene96109.t1 MTR_2g061500 80.620 129 25 0 28 156 1 129 1.51e-73 226
MS.gene96109.t1 MTR_4g103530 84.783 92 14 0 84 175 3 94 4.99e-53 175
MS.gene96109.t1 MTR_7g011710 67.059 85 28 0 82 166 1 85 2.49e-35 127
MS.gene96109.t1 MTR_6g033215 31.336 217 147 1 12 226 78 294 5.53e-33 125
MS.gene96109.t1 MTR_0010s0370 38.202 178 98 4 35 204 4 177 3.71e-32 120
MS.gene96109.t1 MTR_0010s0350 38.202 178 98 4 35 204 4 177 3.71e-32 120
MS.gene96109.t1 MTR_1g036720 33.981 103 68 0 38 140 9 111 9.87e-14 67.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene96109.t1 AT2G17920 25.688 218 146 6 35 243 9 219 2.33e-15 76.6
MS.gene96109.t1 AT3G57587 28.313 166 111 4 82 243 58 219 3.57e-15 76.6
MS.gene96109.t1 AT2G41590 27.711 166 112 4 82 243 58 219 2.89e-14 73.9
MS.gene96109.t1 AT3G31430 25.532 235 156 8 21 242 83 311 1.42e-13 71.6
MS.gene96109.t1 AT5G18636 28.169 142 98 2 104 243 80 219 1.28e-12 68.9
MS.gene96109.t1 AT5G25200 28.169 142 98 2 104 243 80 219 1.29e-12 68.9
MS.gene96109.t1 AT2G02103 28.873 142 97 2 104 243 80 219 1.31e-12 69.3

Find 103 sgRNAs with CRISPR-Local

Find 168 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AGAAGCCAGCAGATGTATTT+TGG 0.145079 4.2:-50968882 None:intergenic
CTTTAGCTAGCACCGCATTC+TGG 0.193213 4.2:-50968835 None:intergenic
CCAAGTCATCCTTTGGGGCT+TGG 0.235303 4.2:+50968938 MS.gene96109:CDS
AGATATACGCTCGCTGAAAT+AGG 0.261512 4.2:-50968063 None:intergenic
GTTCCAAACCTTTGCGTAAC+TGG 0.267932 4.2:-50968623 None:intergenic
CATTCTCGAACATTGTCTTA+TGG 0.269839 4.2:+50968007 MS.gene96109:CDS
CCTATGCCTTGACCTACTTT+TGG 0.278124 4.2:-50968323 None:intergenic
CATCGACTTTGTGGTGAAAC+TGG 0.315582 4.2:-50968148 None:intergenic
CCTGCTTCCAAGTCATCCTT+TGG 0.318496 4.2:+50968931 MS.gene96109:CDS
CTGATGATGGCGTCCGCAAT+TGG 0.330887 4.2:+50968585 MS.gene96109:CDS
CGATAAGATCCATGGTAATA+TGG 0.335879 4.2:-50968214 None:intergenic
TTCATTCCTGCAGAGCGATT+TGG 0.343053 4.2:-50969467 None:intergenic
TCGAGAATGGCAGCGTTATC+AGG 0.354445 4.2:-50967993 None:intergenic
CAGGTTCGGTTTCAAGGTTA+AGG 0.359894 4.2:-50967974 None:intergenic
CTGCTTCCAAGTCATCCTTT+GGG 0.362390 4.2:+50968932 MS.gene96109:CDS
ATTCTCGAACATTGTCTTAT+GGG 0.370010 4.2:+50968008 MS.gene96109:CDS
CAAGGCATAGGAAGCTTCTT+AGG 0.376769 4.2:+50968335 MS.gene96109:CDS
CAACCAACGGTTTGTGGCAG+CGG 0.377733 4.2:-50968645 None:intergenic
CGTTATCAGGTTCGGTTTCA+AGG 0.381639 4.2:-50967980 None:intergenic
CAAGTTCACGGTCTGCCTTT+CGG 0.389727 4.2:+50968290 MS.gene96109:CDS
TGATGATGGCGTCCGCAATT+GGG 0.395960 4.2:+50968586 MS.gene96109:CDS
AGCGACTCTGACACGCATAT+TGG 0.407533 4.2:-50968399 None:intergenic
AAACCAGTTACGCAAAGGTT+TGG 0.415112 4.2:+50968620 MS.gene96109:CDS
CTCCTTCCCAGTCGATCTCA+TGG 0.418743 4.2:+50967899 MS.gene96109:CDS
TTCGAATTAGATTCTGATGA+TGG 0.422237 4.2:+50968572 MS.gene96109:CDS
TGTCACAGGTGTGGATTGAT+TGG 0.423731 4.2:+50968521 MS.gene96109:CDS
TGCTTCCAAAATACATCTGC+TGG 0.424718 4.2:+50968877 MS.gene96109:CDS
AGGTGAGATCGTCAAGGTTA+AGG 0.429626 4.2:-50967932 None:intergenic
AGGTTCGGTTTCAAGGTTAA+GGG 0.437088 4.2:-50967973 None:intergenic
TTTAGCTAGCACCGCATTCT+GGG 0.437460 4.2:-50968834 None:intergenic
GTGATCGTAACCTTCTTGCC+TGG 0.441945 4.2:-50968098 None:intergenic
TGGTTCAGTGGGACAAAGCT+AGG 0.444057 4.2:-50968251 None:intergenic
ATCTTGCAACCAACGGTTTG+TGG 0.450416 4.2:-50968651 None:intergenic
GTAACGGTCAAACACTGATT+TGG 0.459048 4.2:-50968120 None:intergenic
CCAAGCCCCAAAGGATGACT+TGG 0.469128 4.2:-50968938 None:intergenic
TATAGACCAAATCGCTCTGC+AGG 0.469827 4.2:+50969461 MS.gene96109:intron
GAAGGATCCATGAGATCGAC+TGG 0.470594 4.2:-50967906 None:intergenic
ACCATGGATCTTATCGCTCC+TGG 0.475299 4.2:+50968221 MS.gene96109:CDS
AATGGCAGCGTTATCAGGTT+CGG 0.477202 4.2:-50967988 None:intergenic
TGTGACATAGAAGCAGGAAT+AGG 0.487822 4.2:-50968674 None:intergenic
CAAGCAGGTGTTGCTTCTGT+TGG 0.488645 4.2:+50968707 MS.gene96109:CDS
CCAAAGGATGACTTGGAAGC+AGG 0.490656 4.2:-50968931 None:intergenic
TCATTAGATTCACTTGAAAG+TGG 0.496760 4.2:-50968974 None:intergenic
AGTAACAAAAGCACCGTTTG+TGG 0.497986 4.2:-50968465 None:intergenic
CTGCTGGCTTCTTCTTCCAC+AGG 0.507471 4.2:+50968893 MS.gene96109:CDS
GATGATGGCGTCCGCAATTG+GGG 0.507633 4.2:+50968587 MS.gene96109:CDS
TGGCGAGTCCGTGCCACAAA+CGG 0.511841 4.2:+50968452 MS.gene96109:CDS
CACCTTCAGTAGCTGCAGTT+CGG 0.513939 4.2:-50968730 None:intergenic
TGCTTCCAAGTCATCCTTTG+GGG 0.514970 4.2:+50968933 MS.gene96109:CDS
TCACTTGAAAGTGGAGCAGC+AGG 0.515569 4.2:-50968965 None:intergenic
GGTTCAGTGGGACAAAGCTA+GGG 0.531396 4.2:-50968250 None:intergenic
ATATCTCGTGCATGGAAGCC+AGG 0.533414 4.2:+50968080 MS.gene96109:CDS
GGATTCATCCAACCAAAAGT+AGG 0.533518 4.2:+50968311 MS.gene96109:CDS
AACTGCAGCTACTGAAGGTG+TGG 0.539423 4.2:+50968733 MS.gene96109:CDS
GCAGGTAAGCCAAGCCCCAA+AGG 0.549880 4.2:-50968947 None:intergenic
GTTTGATGTGACATAGAAGC+AGG 0.552176 4.2:-50968680 None:intergenic
TACGTGCAACATCGACTTTG+TGG 0.554659 4.2:-50968157 None:intergenic
GAGATCGTCAAGGTTAAGGT+TGG 0.556040 4.2:-50967928 None:intergenic
CAACAGAAGCAACACCTGCT+TGG 0.556389 4.2:-50968706 None:intergenic
TCATGTCAGCTCCCCAATTG+CGG 0.560990 4.2:-50968598 None:intergenic
CATAAGACAATGTTCGAGAA+TGG 0.561219 4.2:-50968006 None:intergenic
GTATCCTTGATGAGGGTCCA+TGG 0.562683 4.2:+50968177 MS.gene96109:CDS
GAACCGCTGCCACAAACCGT+TGG 0.569652 4.2:+50968642 MS.gene96109:CDS
TGTTGCACGTATCCTTGATG+AGG 0.571188 4.2:+50968169 MS.gene96109:CDS
CTTTGTGGTGAAACTGGAAG+AGG 0.574937 4.2:-50968142 None:intergenic
CATGAGATCGACTGGGAAGG+AGG 0.576196 4.2:-50967898 None:intergenic
ACAAAGCTAGGGGCGACACC+AGG 0.577469 4.2:-50968239 None:intergenic
AGAATCTAATTCGAACAACT+CGG 0.580030 4.2:-50968564 None:intergenic
CAAAAGCACCGTTTGTGGCA+CGG 0.590707 4.2:-50968460 None:intergenic
AAACGACGAGGGAAACAGCA+AGG 0.592522 4.2:-50967875 None:intergenic
TGCATGGAAGCCAGGCAAGA+AGG 0.592817 4.2:+50968088 MS.gene96109:CDS
TCGTCAAGGTTAAGGTTGGA+AGG 0.593308 4.2:-50967924 None:intergenic
GTGCTAGCTAAAGAAGGTAA+AGG 0.593767 4.2:+50968845 MS.gene96109:CDS
ACAACCATGGACCCTCATCA+AGG 0.599966 4.2:-50968181 None:intergenic
ATCCATGAGATCGACTGGGA+AGG 0.600804 4.2:-50967901 None:intergenic
GAATCTAATTCGAACAACTC+GGG 0.601829 4.2:-50968563 None:intergenic
TCACATCAAACTCACCAAGC+AGG 0.603196 4.2:+50968692 MS.gene96109:CDS
TGATAATTTCCATATTACCA+TGG 0.603410 4.2:+50968205 MS.gene96109:CDS
AATGCGGTGCTAGCTAAAGA+AGG 0.603825 4.2:+50968839 MS.gene96109:CDS
GCAAGGAAACAGAGGAGACT+CGG 0.606376 4.2:-50967858 None:intergenic
GGGAAACAGCAAGGAAACAG+AGG 0.606669 4.2:-50967866 None:intergenic
GGCCGAACTGCAGCTACTGA+AGG 0.610869 4.2:+50968728 MS.gene96109:CDS
ATCCAACCAAAAGTAGGTCA+AGG 0.617189 4.2:+50968317 MS.gene96109:CDS
CCAAAAGTAGGTCAAGGCAT+AGG 0.620618 4.2:+50968323 MS.gene96109:CDS
ACCAGGAGCGATAAGATCCA+TGG 0.622459 4.2:-50968222 None:intergenic
GGTGAAACTGGAAGAGGTAA+CGG 0.623079 4.2:-50968136 None:intergenic
GTTGCACGTATCCTTGATGA+GGG 0.623154 4.2:+50968170 MS.gene96109:CDS
ATCTAATGATGACATACAAG+AGG 0.624350 4.2:+50968988 MS.gene96109:CDS
GCTAGTAGCTGAAGTACCTG+TGG 0.624706 4.2:-50968909 None:intergenic
GCGAGCGTATATCTCGTGCA+TGG 0.632001 4.2:+50968072 MS.gene96109:CDS
ACATGAAACCAGTTACGCAA+AGG 0.638258 4.2:+50968615 MS.gene96109:CDS
TGGAAATTATCATACAACCA+TGG 0.648806 4.2:-50968194 None:intergenic
GAATAGGATCTTGCAACCAA+CGG 0.654985 4.2:-50968658 None:intergenic
ATTGAATCTCACGATCAGCA+AGG 0.657379 4.2:-50968037 None:intergenic
ACTGCAGCTACTGAAGGTGT+GGG 0.661239 4.2:+50968734 MS.gene96109:CDS
GGTTAAACTCTCTTCAGACA+AGG 0.669576 4.2:-50967952 None:intergenic
TTTGGTTGGATGAATCCGAA+AGG 0.672828 4.2:-50968305 None:intergenic
GTTCAGTGGGACAAAGCTAG+GGG 0.677969 4.2:-50968249 None:intergenic
CTGGGAAGGAGGAAACGACG+AGG 0.680968 4.2:-50967887 None:intergenic
TGGGAAGGAGGAAACGACGA+GGG 0.681663 4.2:-50967886 None:intergenic
AAGGATCCATGAGATCGACT+GGG 0.682195 4.2:-50967905 None:intergenic
CAGACAAGGTGAGATCGTCA+AGG 0.696150 4.2:-50967938 None:intergenic
TGTGCTTGCTTCCCAGAATG+CGG 0.709285 4.2:+50968823 MS.gene96109:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTAAATATGAAAAATT+GGG + chr4.2:50968491-50968510 MS.gene96109:CDS 10.0%
!! GTAAATAAAAAAAAATTACA+GGG + chr4.2:50969287-50969306 MS.gene96109:intron 10.0%
!! TAAATAAAAAAAAATTACAG+GGG + chr4.2:50969288-50969307 MS.gene96109:intron 10.0%
!! TAACTTTAAATATGAAAAAT+TGG + chr4.2:50968490-50968509 MS.gene96109:CDS 10.0%
!!! AAAACAATTTTTTTTTTACA+GGG - chr4.2:50969149-50969168 None:intergenic 10.0%
!! AAATAAAAAAAAATTACAGG+GGG + chr4.2:50969289-50969308 MS.gene96109:intron 15.0%
!! ATCAAAAAAAAAAAAATTGC+AGG - chr4.2:50969120-50969139 None:intergenic 15.0%
!! GGTAAATAAAAAAAAATTAC+AGG + chr4.2:50969286-50969305 MS.gene96109:intron 15.0%
!! TCAAAAAAAAAAAAATTGCA+GGG - chr4.2:50969119-50969138 None:intergenic 15.0%
!! TTGACTTTAATAGATATTAT+GGG + chr4.2:50969434-50969453 MS.gene96109:intron 15.0%
!!! AAATAAAATTGGTTTTTGTT+TGG - chr4.2:50969192-50969211 None:intergenic 15.0%
!!! CAAAACAATTTTTTTTTTAC+AGG - chr4.2:50969150-50969169 None:intergenic 15.0%
!!! TTGTTAAGTTATTCATTTTT+GGG - chr4.2:50969361-50969380 None:intergenic 15.0%
!!! TTTGACTTTAATAGATATTA+TGG + chr4.2:50969433-50969452 MS.gene96109:intron 15.0%
!!! TTTGTTAAGTTATTCATTTT+TGG - chr4.2:50969362-50969381 None:intergenic 15.0%
!! AAAAAAAAAAAAATTGCAGG+GGG - chr4.2:50969117-50969136 None:intergenic 20.0%
!! AATAAAAAAAAATTACAGGG+GGG + chr4.2:50969290-50969309 MS.gene96109:intron 20.0%
!! CAAAAAAAAAAAAATTGCAG+GGG - chr4.2:50969118-50969137 None:intergenic 20.0%
!!! CAAAACCAATTTTTTTTACA+GGG + chr4.2:50969262-50969281 MS.gene96109:intron 20.0%
!!! TCAAAACCAATTTTTTTTAC+AGG + chr4.2:50969261-50969280 MS.gene96109:intron 20.0%
!!! TTATTCATTTTTGGGTTAAT+AGG - chr4.2:50969353-50969372 None:intergenic 20.0%
! AAAAAAAAAAAATTGCAGGG+GGG - chr4.2:50969116-50969135 None:intergenic 25.0%
! AAAAATCAATATGGTTCAGT+GGG - chr4.2:50968265-50968284 None:intergenic 25.0%
! TGATAATTTCCATATTACCA+TGG + chr4.2:50968205-50968224 MS.gene96109:CDS 25.0%
!!! ACAATTTTTTTTTTACAGGG+AGG - chr4.2:50969146-50969165 None:intergenic 25.0%
!!! ACTGAACCATATTGATTTTT+GGG + chr4.2:50968265-50968284 MS.gene96109:CDS 25.0%
AGAAATGACCTATATTACAG+GGG + chr4.2:50969319-50969338 MS.gene96109:intron 30.0%
AGAATCTAATTCGAACAACT+CGG - chr4.2:50968567-50968586 None:intergenic 30.0%
ATCTAATGATGACATACAAG+AGG + chr4.2:50968988-50969007 MS.gene96109:CDS 30.0%
ATTCTCGAACATTGTCTTAT+GGG + chr4.2:50968008-50968027 MS.gene96109:CDS 30.0%
CAAAAATCAATATGGTTCAG+TGG - chr4.2:50968266-50968285 None:intergenic 30.0%
CAGAAATGACCTATATTACA+GGG + chr4.2:50969318-50969337 MS.gene96109:intron 30.0%
CTTGAACCCAAAAATCAATA+TGG - chr4.2:50968274-50968293 None:intergenic 30.0%
TACCTCCCTGTAAAAAAAAT+TGG - chr4.2:50969270-50969289 None:intergenic 30.0%
TCATTAGATTCACTTGAAAG+TGG - chr4.2:50968977-50968996 None:intergenic 30.0%
TGGAAATTATCATACAACCA+TGG - chr4.2:50968197-50968216 None:intergenic 30.0%
TTCGAATTAGATTCTGATGA+TGG + chr4.2:50968572-50968591 MS.gene96109:CDS 30.0%
!! CGTTTTTGTTTGACCTATTA+GGG + chr4.2:50969232-50969251 MS.gene96109:intron 30.0%
!! GTTTTTGTTTGACCTATTAG+GGG + chr4.2:50969233-50969252 MS.gene96109:intron 30.0%
!! GTTTTTGTTTGGCCTATTAA+GGG - chr4.2:50969181-50969200 None:intergenic 30.0%
!! TAGGTCATTTCTGGTTTAAT+AGG + chr4.2:50969054-50969073 MS.gene96109:intron 30.0%
!! TCGTTTTTGTTTGACCTATT+AGG + chr4.2:50969231-50969250 MS.gene96109:intron 30.0%
!! TTTTTGTTTGACCTATTAGG+GGG + chr4.2:50969234-50969253 MS.gene96109:intron 30.0%
!! TTTTTGTTTGGCCTATTAAG+GGG - chr4.2:50969180-50969199 None:intergenic 30.0%
!!! AACCAATTTTTTTTACAGGG+AGG + chr4.2:50969265-50969284 MS.gene96109:intron 30.0%
!!! CACTGAACCATATTGATTTT+TGG + chr4.2:50968264-50968283 MS.gene96109:CDS 30.0%
CATAAGACAATGTTCGAGAA+TGG - chr4.2:50968009-50968028 None:intergenic 35.0%
CATTCTCGAACATTGTCTTA+TGG + chr4.2:50968007-50968026 MS.gene96109:CDS 35.0%
CCAGAAATGACCTATATTAC+AGG + chr4.2:50969317-50969336 MS.gene96109:intron 35.0%
CCAGAAATGACCTATATTAC+AGG - chr4.2:50969336-50969317 None:intergenic 35.0%
CCTGTAATATAGGTCATTTC+CGG + chr4.2:50969045-50969064 MS.gene96109:intron 35.0%
CCTGTAATATAGGTCATTTC+TGG + chr4.2:50969045-50969064 MS.gene96109:intron 35.0%
CCTGTAATATAGGTCATTTC+TGG - chr4.2:50969064-50969045 None:intergenic 35.0%
CGATAAGATCCATGGTAATA+TGG - chr4.2:50968217-50968236 None:intergenic 35.0%
GAAATGACCTATATTACAGG+AGG - chr4.2:50969339-50969320 None:intergenic 35.0%
GAAATGACCTATATTACAGG+GGG + chr4.2:50969320-50969339 MS.gene96109:intron 35.0%
GAATCTAATTCGAACAACTC+GGG - chr4.2:50968566-50968585 None:intergenic 35.0%
GTCTTTACCTCCTGTAATAT+AGG + chr4.2:50969035-50969054 MS.gene96109:intron 35.0%
! TCTTTTACCCCCTGTAATAT+AGG - chr4.2:50969330-50969349 None:intergenic 35.0%
! TTTGATTTACCCCCCTTAAT+AGG + chr4.2:50969166-50969185 MS.gene96109:intron 35.0%
!! GGTTTTTGTTTGGCCTATTA+AGG - chr4.2:50969182-50969201 None:intergenic 35.0%
!! TTTTGTTTGGCCTATTAAGG+GGG - chr4.2:50969179-50969198 None:intergenic 35.0%
!!! GATTTTTGGGTTCAAGTTCA+CGG + chr4.2:50968278-50968297 MS.gene96109:CDS 35.0%
!!! GTTTTGATTTACCCCCTAAT+AGG - chr4.2:50969248-50969267 None:intergenic 35.0%
AAACCAGTTACGCAAAGGTT+TGG + chr4.2:50968620-50968639 MS.gene96109:CDS 40.0%
ACATGAAACCAGTTACGCAA+AGG + chr4.2:50968615-50968634 MS.gene96109:CDS 40.0%
AGATATACGCTCGCTGAAAT+AGG - chr4.2:50968066-50968085 None:intergenic 40.0%
ATCCAACCAAAAGTAGGTCA+AGG + chr4.2:50968317-50968336 MS.gene96109:CDS 40.0%
ATTGAATCTCACGATCAGCA+AGG - chr4.2:50968040-50968059 None:intergenic 40.0%
CCGGAAATGACCTATATTAC+AGG - chr4.2:50969089-50969108 None:intergenic 40.0%
GAATAGGATCTTGCAACCAA+CGG - chr4.2:50968661-50968680 None:intergenic 40.0%
GATGACATACAAGAGGAAAG+TGG + chr4.2:50968995-50969014 MS.gene96109:intron 40.0%
GGATTCATCCAACCAAAAGT+AGG + chr4.2:50968311-50968330 MS.gene96109:CDS 40.0%
GGTTAAACTCTCTTCAGACA+AGG - chr4.2:50967955-50967974 None:intergenic 40.0%
GTGCTAGCTAAAGAAGGTAA+AGG + chr4.2:50968845-50968864 MS.gene96109:CDS 40.0%
GTTTGATGTGACATAGAAGC+AGG - chr4.2:50968683-50968702 None:intergenic 40.0%
TGCTTCCAAAATACATCTGC+TGG + chr4.2:50968877-50968896 MS.gene96109:CDS 40.0%
TGTGACATAGAAGCAGGAAT+AGG - chr4.2:50968677-50968696 None:intergenic 40.0%
TTTGGTTGGATGAATCCGAA+AGG - chr4.2:50968308-50968327 None:intergenic 40.0%
TTTGTTTGGCCTATTAAGGG+GGG - chr4.2:50969178-50969197 None:intergenic 40.0%
! AATTGGGTGTGTTTTGTCAC+AGG + chr4.2:50968507-50968526 MS.gene96109:CDS 40.0%
! AGAAGCCAGCAGATGTATTT+TGG - chr4.2:50968885-50968904 None:intergenic 40.0%
! AGGTTCGGTTTCAAGGTTAA+GGG - chr4.2:50967976-50967995 None:intergenic 40.0%
! AGTGGCTCGGATTTGAATAT+AGG + chr4.2:50969013-50969032 MS.gene96109:intron 40.0%
! GTAACGGTCAAACACTGATT+TGG - chr4.2:50968123-50968142 None:intergenic 40.0%
!! AGTAACAAAAGCACCGTTTG+TGG - chr4.2:50968468-50968487 None:intergenic 40.0%
AAAATTGCAGGGGGGAAAAC+CGG - chr4.2:50969108-50969127 None:intergenic 45.0%
AAGGATCCATGAGATCGACT+GGG - chr4.2:50967908-50967927 None:intergenic 45.0%
AATGGCAGCGTTATCAGGTT+CGG - chr4.2:50967991-50968010 None:intergenic 45.0%
AGGTGAGATCGTCAAGGTTA+AGG - chr4.2:50967935-50967954 None:intergenic 45.0%
ATCTTGCAACCAACGGTTTG+TGG - chr4.2:50968654-50968673 None:intergenic 45.0%
CAAGGCATAGGAAGCTTCTT+AGG + chr4.2:50968335-50968354 MS.gene96109:CDS 45.0%
CATACAAGAGGAAAGTGGCT+CGG + chr4.2:50969000-50969019 MS.gene96109:intron 45.0%
CCAAAAGTAGGTCAAGGCAT+AGG + chr4.2:50968323-50968342 MS.gene96109:CDS 45.0%
CTGCTTCCAAGTCATCCTTT+GGG + chr4.2:50968932-50968951 MS.gene96109:CDS 45.0%
CTGTCCCACTGAAAAAAGAG+TGG + chr4.2:50968432-50968451 MS.gene96109:CDS 45.0%
CTTTGTGGTGAAACTGGAAG+AGG - chr4.2:50968145-50968164 None:intergenic 45.0%
GAGATCGTCAAGGTTAAGGT+TGG - chr4.2:50967931-50967950 None:intergenic 45.0%
GGTGAAACTGGAAGAGGTAA+CGG - chr4.2:50968139-50968158 None:intergenic 45.0%
GTTCCAAACCTTTGCGTAAC+TGG - chr4.2:50968626-50968645 None:intergenic 45.0%
GTTGCACGTATCCTTGATGA+GGG + chr4.2:50968170-50968189 MS.gene96109:CDS 45.0%
TATAGACCAAATCGCTCTGC+AGG + chr4.2:50969461-50969480 MS.gene96109:intron 45.0%
TCACATCAAACTCACCAAGC+AGG + chr4.2:50968692-50968711 MS.gene96109:CDS 45.0%
TCGTCAAGGTTAAGGTTGGA+AGG - chr4.2:50967927-50967946 None:intergenic 45.0%
TGCTTCCAAGTCATCCTTTG+GGG + chr4.2:50968933-50968952 MS.gene96109:CDS 45.0%
TGTTGCACGTATCCTTGATG+AGG + chr4.2:50968169-50968188 MS.gene96109:CDS 45.0%
TTTAGCTAGCACCGCATTCT+GGG - chr4.2:50968837-50968856 None:intergenic 45.0%
! AATGCGGTGCTAGCTAAAGA+AGG + chr4.2:50968839-50968858 MS.gene96109:CDS 45.0%
! CAGGTTCGGTTTCAAGGTTA+AGG - chr4.2:50967977-50967996 None:intergenic 45.0%
! CCTATGCCTTGACCTACTTT+TGG - chr4.2:50968326-50968345 None:intergenic 45.0%
! CGTTATCAGGTTCGGTTTCA+AGG - chr4.2:50967983-50968002 None:intergenic 45.0%
! TGTCACAGGTGTGGATTGAT+TGG + chr4.2:50968521-50968540 MS.gene96109:CDS 45.0%
!! CATCGACTTTGTGGTGAAAC+TGG - chr4.2:50968151-50968170 None:intergenic 45.0%
!! TACGTGCAACATCGACTTTG+TGG - chr4.2:50968160-50968179 None:intergenic 45.0%
!! TGCCTTGACCTACTTTTGGT+TGG - chr4.2:50968322-50968341 None:intergenic 45.0%
!!! ACTCGCCACTCTTTTTTCAG+TGG - chr4.2:50968440-50968459 None:intergenic 45.0%
!!! CTCGCCACTCTTTTTTCAGT+GGG - chr4.2:50968439-50968458 None:intergenic 45.0%
!! TGAAAAAATAAAAATAAAAT+TGG - chr4.2:50969203-50969222 None:intergenic 5.0%
AAACGACGAGGGAAACAGCA+AGG - chr4.2:50967878-50967897 None:intergenic 50.0%
AACTGCAGCTACTGAAGGTG+TGG + chr4.2:50968733-50968752 MS.gene96109:CDS 50.0%
ACAACCATGGACCCTCATCA+AGG - chr4.2:50968184-50968203 None:intergenic 50.0%
ACCAGGAGCGATAAGATCCA+TGG - chr4.2:50968225-50968244 None:intergenic 50.0%
ACCATGGATCTTATCGCTCC+TGG + chr4.2:50968221-50968240 MS.gene96109:CDS 50.0%
AGCGACTCTGACACGCATAT+TGG - chr4.2:50968402-50968421 None:intergenic 50.0%
ATATCTCGTGCATGGAAGCC+AGG + chr4.2:50968080-50968099 MS.gene96109:CDS 50.0%
ATCCATGAGATCGACTGGGA+AGG - chr4.2:50967904-50967923 None:intergenic 50.0%
CAACAGAAGCAACACCTGCT+TGG - chr4.2:50968709-50968728 None:intergenic 50.0%
CAAGTTCACGGTCTGCCTTT+CGG + chr4.2:50968290-50968309 MS.gene96109:CDS 50.0%
CACCTTCAGTAGCTGCAGTT+CGG - chr4.2:50968733-50968752 None:intergenic 50.0%
CAGACAAGGTGAGATCGTCA+AGG - chr4.2:50967941-50967960 None:intergenic 50.0%
CCTGCTTCCAAGTCATCCTT+TGG + chr4.2:50968931-50968950 MS.gene96109:CDS 50.0%
CTTTAGCTAGCACCGCATTC+TGG - chr4.2:50968838-50968857 None:intergenic 50.0%
GAAGGATCCATGAGATCGAC+TGG - chr4.2:50967909-50967928 None:intergenic 50.0%
GCAAGGAAACAGAGGAGACT+CGG - chr4.2:50967861-50967880 None:intergenic 50.0%
GGGAAACAGCAAGGAAACAG+AGG - chr4.2:50967869-50967888 None:intergenic 50.0%
GGTTCAGTGGGACAAAGCTA+GGG - chr4.2:50968253-50968272 None:intergenic 50.0%
GTATCCTTGATGAGGGTCCA+TGG + chr4.2:50968177-50968196 MS.gene96109:CDS 50.0%
GTGATCGTAACCTTCTTGCC+TGG - chr4.2:50968101-50968120 None:intergenic 50.0%
GTTCAGTGGGACAAAGCTAG+GGG - chr4.2:50968252-50968271 None:intergenic 50.0%
TCACTTGAAAGTGGAGCAGC+AGG - chr4.2:50968968-50968987 None:intergenic 50.0%
TCATGTCAGCTCCCCAATTG+CGG - chr4.2:50968601-50968620 None:intergenic 50.0%
TCGAGAATGGCAGCGTTATC+AGG - chr4.2:50967996-50968015 None:intergenic 50.0%
TGGTTCAGTGGGACAAAGCT+AGG - chr4.2:50968254-50968273 None:intergenic 50.0%
TGTGCTTGCTTCCCAGAATG+CGG + chr4.2:50968823-50968842 MS.gene96109:CDS 50.0%
! ACTGCAGCTACTGAAGGTGT+GGG + chr4.2:50968734-50968753 MS.gene96109:CDS 50.0%
! CCAAAGGATGACTTGGAAGC+AGG - chr4.2:50968934-50968953 None:intergenic 50.0%
! GCTAGTAGCTGAAGTACCTG+TGG - chr4.2:50968912-50968931 None:intergenic 50.0%
! GGTGTGTTTTGTCACAGGTG+TGG + chr4.2:50968512-50968531 MS.gene96109:CDS 50.0%
!! CAAAAGCACCGTTTGTGGCA+CGG - chr4.2:50968463-50968482 None:intergenic 50.0%
!! CAAGCAGGTGTTGCTTCTGT+TGG + chr4.2:50968707-50968726 MS.gene96109:CDS 50.0%
!! TGATGATGGCGTCCGCAATT+GGG + chr4.2:50968586-50968605 MS.gene96109:CDS 50.0%
CAACCAACGGTTTGTGGCAG+CGG - chr4.2:50968648-50968667 None:intergenic 55.0%
CATGAGATCGACTGGGAAGG+AGG - chr4.2:50967901-50967920 None:intergenic 55.0%
CCAAGCCCCAAAGGATGACT+TGG - chr4.2:50968941-50968960 None:intergenic 55.0%
CTCCTTCCCAGTCGATCTCA+TGG + chr4.2:50967899-50967918 MS.gene96109:CDS 55.0%
GCGAGCGTATATCTCGTGCA+TGG + chr4.2:50968072-50968091 MS.gene96109:CDS 55.0%
TGCATGGAAGCCAGGCAAGA+AGG + chr4.2:50968088-50968107 MS.gene96109:CDS 55.0%
TGGGAAGGAGGAAACGACGA+GGG - chr4.2:50967889-50967908 None:intergenic 55.0%
! GATGATGGCGTCCGCAATTG+GGG + chr4.2:50968587-50968606 MS.gene96109:CDS 55.0%
!! CCAAGTCATCCTTTGGGGCT+TGG + chr4.2:50968938-50968957 MS.gene96109:CDS 55.0%
!! CTGATGATGGCGTCCGCAAT+TGG + chr4.2:50968585-50968604 MS.gene96109:CDS 55.0%
!! CTGCTGGCTTCTTCTTCCAC+AGG + chr4.2:50968893-50968912 MS.gene96109:CDS 55.0%
ACAAAGCTAGGGGCGACACC+AGG - chr4.2:50968242-50968261 None:intergenic 60.0%
CTGGGAAGGAGGAAACGACG+AGG - chr4.2:50967890-50967909 None:intergenic 60.0%
GAACCGCTGCCACAAACCGT+TGG + chr4.2:50968642-50968661 MS.gene96109:CDS 60.0%
GCAGGTAAGCCAAGCCCCAA+AGG - chr4.2:50968950-50968969 None:intergenic 60.0%
GGCCGAACTGCAGCTACTGA+AGG + chr4.2:50968728-50968747 MS.gene96109:CDS 60.0%
TGGCGAGTCCGTGCCACAAA+CGG + chr4.2:50968452-50968471 MS.gene96109:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 50967837 50969488 50967837 ID=MS.gene96109
chr4.2 mRNA 50967837 50969488 50967837 ID=MS.gene96109.t1;Parent=MS.gene96109
chr4.2 exon 50967837 50969013 50967837 ID=MS.gene96109.t1.exon1;Parent=MS.gene96109.t1
chr4.2 CDS 50967837 50969013 50967837 ID=cds.MS.gene96109.t1;Parent=MS.gene96109.t1
chr4.2 exon 50969466 50969488 50969466 ID=MS.gene96109.t1.exon2;Parent=MS.gene96109.t1
chr4.2 CDS 50969466 50969488 50969466 ID=cds.MS.gene96109.t1;Parent=MS.gene96109.t1
Gene Sequence

>MS.gene96109

ATGCGTTTTGCTGTTTCTAAACCGAGTCTCCTCTGTTTCCTTGCTGTTTCCCTCGTCGTTTCCTCCTTCCCAGTCGATCTCATGGATCCTTCCAACCTTAACCTTGACGATCTCACCTTGTCTGAAGAGAGTTTAACCCTTAACCTTGAAACCGAACCTGATAACGCTGCCATTCTCGAACATTGTCTTATGGGTCGAGTCCTTGCTGATCGTGAGATTCAATTTGCCTATTTCAGCGAGCGTATATCTCGTGCATGGAAGCCAGGCAAGAAGGTTACGATCACCAAATCAGTGTTTGACCGTTACCTCTTCCAGTTTCACCACAAAGTCGATGTTGCACGTATCCTTGATGAGGGTCCATGGTTGTATGATAATTTCCATATTACCATGGATCTTATCGCTCCTGGTGTCGCCCCTAGCTTTGTCCCACTGAACCATATTGATTTTTGGGTTCAAGTTCACGGTCTGCCTTTCGGATTCATCCAACCAAAAGTAGGTCAAGGCATAGGAAGCTTCTTAGGCACTTTCAAAGCATATGATAATCGCAACTCAATTCATAGTTCCAATATGCGTGTCAGAGTCGCTATTGTTGTCACTGTCCCACTGAAAAAAGAGTGGCGAGTCCGTGCCACAAACGGTGCTTTTGTTACTGTTAACTTTAAATATGAAAAATTGGGTGTGTTTTGTCACAGGTGTGGATTGATTGGTCATACTGACAAAGTGTGTCCCGAGTTGTTCGAATTAGATTCTGATGATGGCGTCCGCAATTGGGGAGCTGACATGAAACCAGTTACGCAAAGGTTTGGAACCGCTGCCACAAACCGTTGGTTGCAAGATCCTATTCCTGCTTCTATGTCACATCAAACTCACCAAGCAGGTGTTGCTTCTGTTGGCCGAACTGCAGCTACTGAAGGTGTGGGAAATTCTGCAACTTTCAATGATCGTATGCTCACTCTTCAGACGCAACTTACTGCTATGCAACAAGATGTGCTTGCTTCCCAGAATGCGGTGCTAGCTAAAGAAGGTAAAGGTGCAAGTTCTGCTTCCAAAATACATCTGCTGGCTTCTTCTTCCACAGGTACTTCAGCTACTAGCCTGCTTCCAAGTCATCCTTTGGGGCTTGGCTTACCTGCTGCTCCACTTTCAAGTGAATCTAATGATGACATACAAGAGGAAAGTGGCTCGGATTTGAATATAGGTCTTTACCTCCTGTAATATAGGTCATTTCTGGTTTAATAGGTCTTTACCTCCTGTAATATAGGTCATTTCCGGTTTTCCCCCCTGCAATTTTTTTTTTTTTGATTTACCTCCCTGTAAAAAAAAAATTGTTTTGATTTACCCCCCTTAATAGGCCAAACAAAAACCAATTTTATTTTTATTTTTTCAAGAAATTTTCGTTTTTGTTTGACCTATTAGGGGGTAAATCAAAACCAATTTTTTTTACAGGGAGGTAAATAAAAAAAAATTACAGGGGGGAAAACCAGAAATGACCTATATTACAGGGGGTAAAAGACCTATTAACCCAAAAATGAATAACTTAACAAAAGTTCTATAATTTGAAACTTACTTTTATATTTAATTTTAAAAACTCTATCATTTGACTTTAATAGATATTATGGGTAATTATAGACCAAATCGCTCTGCAGGAATGA
Protein sequence

>MS.gene96109.t1

MRFAVSKPSLLCFLAVSLVVSSFPVDLMDPSNLNLDDLTLSEESLTLNLETEPDNAAILEHCLMGRVLADREIQFAYFSERISRAWKPGKKVTITKSVFDRYLFQFHHKVDVARILDEGPWLYDNFHITMDLIAPGVAPSFVPLNHIDFWVQVHGLPFGFIQPKVGQGIGSFLGTFKAYDNRNSIHSSNMRVRVAIVVTVPLKKEWRVRATNGAFVTVNFKYEKLGVFCHRCGLIGHTDKVCPELFELDSDDGVRNWGADMKPVTQRFGTAATNRWLQDPIPASMSHQTHQAGVASVGRTAATEGVGNSATFNDRMLTLQTQLTAMQQDVLASQNAVLAKEGKGASSASKIHLLASSSTGTSATSLLPSHPLGLGLPAAPLSSESNDDIQEENQIALQE