AlfalfaGEDB | MS.gene61452

M. sativa cultivar XinJiangDaYe / MS.gene61452

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61452.t1	PNX72859.1	43.3	404	192	7	1	394	123	499	9.80E-86	327.4

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61452.t1	A0A392MN10	55.3	262	116	1	1	262	24	284	1.3e-87	333.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene013193	MS.gene61452	PPI
MS.gene08106	MS.gene61452	PPI
MS.gene61452	MS.gene80362	PPI
MS.gene61452	MS.gene49057	PPI
MS.gene61452	MS.gene89815	PPI
MS.gene61452	MS.gene018474	PPI
MS.gene61452	MS.gene048088	PPI
MS.gene61452	MS.gene86868	PPI
MS.gene61452	MS.gene058889	PPI
MS.gene23521	MS.gene61452	PPI
MS.gene61452	MS.gene96163	PPI
MS.gene61452	MS.gene034643	PPI
MS.gene87529	MS.gene61452	PPI
MS.gene012748	MS.gene61452	PPI
MS.gene04134	MS.gene61452	PPI
MS.gene83979	MS.gene61452	PPI
MS.gene062067	MS.gene61452	PPI
MS.gene61452	MS.gene012889	PPI
MS.gene61452	MS.gene34463	PPI
MS.gene57044	MS.gene61452	PPI
MS.gene61452	MS.gene042614	PPI
MS.gene61452	MS.gene61499	PPI
MS.gene038676	MS.gene61452	PPI
MS.gene61452	MS.gene51804	PPI
MS.gene042651	MS.gene61452	PPI
MS.gene61452	MS.gene81194	PPI
MS.gene05231	MS.gene61452	PPI
MS.gene61452	MS.gene53904	PPI
MS.gene61452	MS.gene025675	PPI
MS.gene050679	MS.gene61452	PPI
MS.gene61452	MS.gene71637	PPI
MS.gene61452	MS.gene55209	PPI
MS.gene61452	MS.gene26038	PPI
MS.gene20246	MS.gene61452	PPI
MS.gene61452	MS.gene43924	PPI
MS.gene071784	MS.gene61452	PPI
MS.gene61452	MS.gene022929	PPI
MS.gene61452	MS.gene85295	PPI
MS.gene61452	MS.gene007022	PPI
MS.gene61452	MS.gene52349	PPI
MS.gene61452	MS.gene021647	PPI
MS.gene61452	MS.gene047954	PPI
MS.gene61452	MS.gene071784	PPI
MS.gene61452	MS.gene048342	PPI
MS.gene042559	MS.gene61452	PPI
MS.gene22200	MS.gene61452	PPI
MS.gene61452	MS.gene069624	PPI
MS.gene61452	MS.gene013890	PPI
MS.gene26038	MS.gene61452	PPI
MS.gene61452	MS.gene073582	PPI
MS.gene61452	MS.gene016719	PPI
MS.gene61452	MS.gene028714	PPI
MS.gene61452	MS.gene88393	PPI
MS.gene61452	MS.gene042027	PPI
MS.gene61452	MS.gene048706	PPI
MS.gene34812	MS.gene61452	PPI
MS.gene61452	MS.gene69586	PPI
MS.gene61452	MS.gene45083	PPI
MS.gene02896	MS.gene61452	PPI
MS.gene61452	MS.gene049561	PPI
MS.gene61452	MS.gene008658	PPI
MS.gene063214	MS.gene61452	PPI
MS.gene61452	MS.gene63150	PPI
MS.gene61452	MS.gene03936	PPI
MS.gene067340	MS.gene61452	PPI
MS.gene65623	MS.gene61452	PPI
MS.gene61452	MS.gene08708	PPI
MS.gene69495	MS.gene61452	PPI
MS.gene049512	MS.gene61452	PPI
MS.gene61452	MS.gene06164	PPI
MS.gene61452	MS.gene86275	PPI
MS.gene038623	MS.gene61452	PPI
MS.gene05357	MS.gene61452	PPI
MS.gene032311	MS.gene61452	PPI
MS.gene61452	MS.gene037634	PPI
MS.gene61452	MS.gene057828	PPI
MS.gene61452	MS.gene071320	PPI
MS.gene61452	MS.gene000640	PPI
MS.gene86849	MS.gene61452	PPI
MS.gene61452	MS.gene038757	PPI
MS.gene61452	MS.gene000506	PPI
MS.gene61452	MS.gene39956	PPI
MS.gene61452	MS.gene028716	PPI
MS.gene61452	MS.gene74639	PPI
MS.gene61452	MS.gene071133	PPI
MS.gene61452	MS.gene60266	PPI
MS.gene61452	MS.gene98467	PPI
MS.gene021968	MS.gene61452	PPI
MS.gene61452	MS.gene013644	PPI
MS.gene61452	MS.gene024522	PPI
MS.gene61452	MS.gene54051	PPI
MS.gene61452	MS.gene22111	PPI
MS.gene09589	MS.gene61452	PPI
MS.gene61452	MS.gene049610	PPI
MS.gene61452	MS.gene010290	PPI
MS.gene61452	MS.gene37955	PPI
MS.gene43393	MS.gene61452	PPI
MS.gene61452	MS.gene92517	PPI
MS.gene61452	MS.gene34197	PPI
MS.gene61452	MS.gene36069	PPI
MS.gene61452	MS.gene045875	PPI
MS.gene61452	MS.gene010890	PPI
MS.gene61452	MS.gene84790	PPI
MS.gene61452	MS.gene71675	PPI
MS.gene61452	MS.gene061579	PPI
MS.gene61452	MS.gene002590	PPI
MS.gene61452	MS.gene41979	PPI
MS.gene61452	MS.gene04970	PPI
MS.gene048072	MS.gene61452	PPI
MS.gene060386	MS.gene61452	PPI
MS.gene71588	MS.gene61452	PPI
MS.gene61452	MS.gene008927	PPI
MS.gene04749	MS.gene61452	PPI
MS.gene61452	MS.gene060788	PPI
MS.gene61452	MS.gene23521	PPI
MS.gene05436	MS.gene61452	PPI
MS.gene61452	MS.gene41643	PPI
MS.gene61452	MS.gene77990	PPI
MS.gene61452	MS.gene65546	PPI
MS.gene059736	MS.gene61452	PPI
MS.gene61452	MS.gene062511	PPI
MS.gene61452	MS.gene008861	PPI
MS.gene61452	MS.gene027994	PPI
MS.gene61452	MS.gene26433	PPI
MS.gene61452	MS.gene045614	PPI
MS.gene42903	MS.gene61452	PPI
MS.gene61452	MS.gene69584	PPI
MS.gene021965	MS.gene61452	PPI
MS.gene055683	MS.gene61452	PPI
MS.gene61452	MS.gene76420	PPI
MS.gene61452	MS.gene46353	PPI
MS.gene61452	MS.gene65471	PPI
MS.gene048747	MS.gene61452	PPI
MS.gene61452	MS.gene51799	PPI
MS.gene61452	MS.gene61651	PPI
MS.gene61452	MS.gene29406	PPI
MS.gene94654	MS.gene61452	PPI
MS.gene61452	MS.gene09023	PPI
MS.gene61452	MS.gene054508	PPI
MS.gene031967	MS.gene61452	PPI
MS.gene61452	MS.gene025987	PPI
MS.gene61452	MS.gene95960	PPI
MS.gene61452	MS.gene053373	PPI
MS.gene61452	MS.gene041264	PPI
MS.gene97410	MS.gene61452	PPI
MS.gene61452	MS.gene35175	PPI
MS.gene83772	MS.gene61452	PPI
MS.gene32415	MS.gene61452	PPI
MS.gene035626	MS.gene61452	PPI
MS.gene008927	MS.gene61452	PPI
MS.gene045614	MS.gene61452	PPI
MS.gene61452	MS.gene44083	PPI
MS.gene61452	MS.gene072852	PPI
MS.gene63762	MS.gene61452	PPI
MS.gene61452	MS.gene65474	PPI
MS.gene61452	MS.gene018745	PPI
MS.gene61452	MS.gene025401	PPI
MS.gene71675	MS.gene61452	PPI
MS.gene61452	MS.gene015093	PPI
MS.gene30208	MS.gene61452	PPI
MS.gene61452	MS.gene50392	PPI
MS.gene61452	MS.gene22730	PPI
MS.gene61452	MS.gene000379	PPI
MS.gene013676	MS.gene61452	PPI
MS.gene61452	MS.gene032299	PPI
MS.gene61452	MS.gene017586	PPI
MS.gene61452	MS.gene009121	PPI
MS.gene030090	MS.gene61452	PPI
MS.gene95561	MS.gene61452	PPI
MS.gene61452	MS.gene08606	PPI
MS.gene018196	MS.gene61452	PPI
MS.gene065627	MS.gene61452	PPI
MS.gene61452	MS.gene70932	PPI
MS.gene61452	MS.gene09517	PPI
MS.gene61452	MS.gene98756	PPI
MS.gene57019	MS.gene61452	PPI
MS.gene61452	MS.gene87845	PPI
MS.gene018745	MS.gene61452	PPI
MS.gene61452	MS.gene85308	PPI
MS.gene61452	MS.gene64130	PPI
MS.gene61452	MS.gene62249	PPI
MS.gene61452	MS.gene58419	PPI
MS.gene038098	MS.gene61452	PPI
MS.gene61452	MS.gene046089	PPI
MS.gene60059	MS.gene61452	PPI
MS.gene61452	MS.gene053747	PPI
MS.gene61452	MS.gene021555	PPI
MS.gene34197	MS.gene61452	PPI
MS.gene61452	MS.gene05576	PPI
MS.gene008275	MS.gene61452	PPI
MS.gene61452	MS.gene044729	PPI
MS.gene61452	MS.gene60390	PPI
MS.gene064966	MS.gene61452	PPI
MS.gene61452	MS.gene48477	PPI
MS.gene61452	MS.gene039692	PPI
MS.gene61452	MS.gene027169	PPI
MS.gene61452	MS.gene55682	PPI
MS.gene61452	MS.gene81712	PPI
MS.gene61452	MS.gene005120	PPI
MS.gene046089	MS.gene61452	PPI
MS.gene61452	MS.gene055560	PPI
MS.gene047954	MS.gene61452	PPI
MS.gene35175	MS.gene61452	PPI
MS.gene61452	MS.gene024297	PPI
MS.gene036423	MS.gene61452	PPI
MS.gene61452	MS.gene059269	PPI
MS.gene61452	MS.gene35202	PPI
MS.gene61452	MS.gene057934	PPI
MS.gene61452	MS.gene37537	PPI
MS.gene61452	MS.gene024841	PPI
MS.gene61452	MS.gene069786	PPI
MS.gene61452	MS.gene42010	PPI
MS.gene61452	MS.gene025158	PPI
MS.gene61452	MS.gene40828	PPI
MS.gene61452	MS.gene27329	PPI
MS.gene66651	MS.gene61452	PPI
MS.gene61452	MS.gene87529	PPI
MS.gene61452	MS.gene41393	PPI
MS.gene96109	MS.gene61452	PPI
MS.gene61452	MS.gene98471	PPI
MS.gene35202	MS.gene61452	PPI
MS.gene051449	MS.gene61452	PPI
MS.gene61452	MS.gene016632	PPI
MS.gene61452	MS.gene40469	PPI
MS.gene61452	MS.gene65623	PPI
MS.gene61452	MS.gene012748	PPI
MS.gene61452	MS.gene043140	PPI
MS.gene61452	MS.gene02896	PPI
MS.gene30136	MS.gene61452	PPI
MS.gene61452	MS.gene71588	PPI
MS.gene008861	MS.gene61452	PPI
MS.gene61452	MS.gene03149	PPI
MS.gene016102	MS.gene61452	PPI
MS.gene46941	MS.gene61452	PPI
MS.gene96541	MS.gene61452	PPI
MS.gene61452	MS.gene29321	PPI
MS.gene85295	MS.gene61452	PPI
MS.gene61452	MS.gene037049	PPI
MS.gene47792	MS.gene61452	PPI
MS.gene46948	MS.gene61452	PPI
MS.gene61452	MS.gene026680	PPI
MS.gene025873	MS.gene61452	PPI
MS.gene012019	MS.gene61452	PPI
MS.gene61452	MS.gene24278	PPI
MS.gene61452	MS.gene34618	PPI
MS.gene05358	MS.gene61452	PPI
MS.gene61452	MS.gene59460	PPI
MS.gene61452	MS.gene019165	PPI
MS.gene93396	MS.gene61452	PPI
MS.gene61452	MS.gene060773	PPI
MS.gene61452	MS.gene66650	PPI
MS.gene61452	MS.gene08713	PPI
MS.gene61452	MS.gene038394	PPI
MS.gene61452	MS.gene019001	PPI
MS.gene61452	MS.gene024405	PPI
MS.gene010656	MS.gene61452	PPI
MS.gene000629	MS.gene61452	PPI
MS.gene61452	MS.gene021965	PPI
MS.gene61452	MS.gene24070	PPI
MS.gene61452	MS.gene73261	PPI
MS.gene61452	MS.gene059736	PPI
MS.gene61452	MS.gene85538	PPI
MS.gene05319	MS.gene61452	PPI
MS.gene61452	MS.gene26844	PPI
MS.gene61452	MS.gene64178	PPI
MS.gene61452	MS.gene055546	PPI
MS.gene56991	MS.gene61452	PPI
MS.gene61452	MS.gene96174	PPI
MS.gene61452	MS.gene074257	PPI
MS.gene055223	MS.gene61452	PPI
MS.gene61452	MS.gene073573	PPI
MS.gene61452	MS.gene28718	PPI
MS.gene28545	MS.gene61452	PPI
MS.gene61452	MS.gene44144	PPI
MS.gene053747	MS.gene61452	PPI
MS.gene61452	MS.gene060718	PPI
MS.gene61452	MS.gene63743	PPI
MS.gene65471	MS.gene61452	PPI
MS.gene061850	MS.gene61452	PPI
MS.gene61452	MS.gene068629	PPI
MS.gene61452	MS.gene004417	PPI
MS.gene61452	MS.gene009781	PPI
MS.gene61452	MS.gene019177	PPI
MS.gene98616	MS.gene61452	PPI
MS.gene61452	MS.gene21066	PPI
MS.gene61452	MS.gene009213	PPI
MS.gene61452	MS.gene98127	PPI
MS.gene61452	MS.gene003264	PPI
MS.gene61452	MS.gene054652	PPI
MS.gene61452	MS.gene000634	PPI
MS.gene69490	MS.gene61452	PPI
MS.gene015248	MS.gene61452	PPI
MS.gene61452	MS.gene86849	PPI
MS.gene61452	MS.gene049512	PPI
MS.gene72520	MS.gene61452	PPI
MS.gene44083	MS.gene61452	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61452.t1	MTR_3g053920	33.908	348	155	5	3	348	6	280	3.46e-64	207
MS.gene61452.t1	MTR_2g061500	51.200	125	61	0	2	126	5	129	2.58e-43	149
MS.gene61452.t1	MTR_6g033215	36.517	178	111	1	21	196	117	294	1.87e-36	135
MS.gene61452.t1	MTR_0010s0370	35.227	176	108	3	4	174	3	177	1.77e-30	116
MS.gene61452.t1	MTR_0010s0350	35.227	176	108	3	4	174	3	177	1.77e-30	116
MS.gene61452.t1	MTR_4g103530	51.613	93	45	0	52	144	1	93	1.04e-27	108
MS.gene61452.t1	MTR_7g011710	51.765	85	41	0	52	136	1	85	1.93e-23	96.3
MS.gene61452.t1	MTR_1g036720	32.353	102	67	1	4	103	3	104	2.28e-12	63.9

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene61452.t1	AT5G36228	28.395	162	103	4	68	222	73	228	1.29e-14	75.5
MS.gene61452.t1	AT2G17920	27.815	151	98	4	68	212	73	218	3.86e-13	70.5
MS.gene61452.t1	AT3G57587	27.083	144	95	3	74	212	80	218	1.17e-12	69.3
MS.gene61452.t1	AT3G31430	28.767	146	94	4	71	212	172	311	1.08e-11	66.2

Find 120 sgRNAs with CRISPR-Local

Find 220 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCCTTAACTTTCTTGATATT+TGG	0.063914	8.3:-13715455	MS.gene61452:CDS
CACTGTTTGTCGAGGAATTA+AGG	0.177097	8.3:+13714774	None:intergenic
GGGACTGTTGGGATTGATTT+AGG	0.197835	8.3:-13714616	MS.gene61452:CDS
TGCTATGCTTGTGGTATATT+AGG	0.214654	8.3:-13715198	MS.gene61452:CDS
AACAGACATATCAAACTTTA+TGG	0.217964	8.3:+13714888	None:intergenic
CCTTAACTTTCTTGATATTT+GGG	0.221031	8.3:-13715454	MS.gene61452:CDS
GCTATGCTTGTGGTATATTA+GGG	0.264828	8.3:-13715197	MS.gene61452:CDS
CATCAAGTCTGTGATTAAAT+TGG	0.265185	8.3:+13715571	None:intergenic
CAGGTCCACAATCTGCCTTT+TGG	0.326653	8.3:-13715429	MS.gene61452:CDS
GCTATGCAAGTGGATGAATT+AGG	0.351606	8.3:-13714637	MS.gene61452:CDS
TCATGCTATTGCTCAACATC+TGG	0.354492	8.3:-13715385	MS.gene61452:CDS
GCAATAGCATGACCGACTTT+TGG	0.361528	8.3:+13715396	None:intergenic
AGGAGGTGAGTGGGTAACTA+TGG	0.367680	8.3:-13715250	MS.gene61452:CDS
GCGAATTCAATGGTAGTTCT+AGG	0.370560	8.3:-13714946	MS.gene61452:CDS
TAGGTTCTGTTGATGTTAGT+GGG	0.375575	8.3:-13714597	MS.gene61452:CDS
TTCTTGACCACTGCTTAGTT+GGG	0.377802	8.3:-13715710	MS.gene61452:CDS
GTGAAAGGCTGAGCCGGCTT+TGG	0.377913	8.3:-13715647	MS.gene61452:CDS
CTTTGCGAGGATCAGGCTTA+AGG	0.381261	8.3:+13715103	None:intergenic
GCGCATCACCGGGAGCTTCA+AGG	0.384358	8.3:+13714746	None:intergenic
CCCAAATATCAAGAAAGTTA+AGG	0.385422	8.3:+13715454	None:intergenic
CCGGCTTTGGCGTCCGGTGA+AGG	0.389421	8.3:-13715634	MS.gene61452:CDS
ATTCTTGACCACTGCTTAGT+TGG	0.392124	8.3:-13715711	MS.gene61452:CDS
CTTTCTTGATATTTGGGTGC+AGG	0.394108	8.3:-13715448	MS.gene61452:CDS
TGATGATGGAGTGCGAGGTT+GGG	0.396657	8.3:-13715133	MS.gene61452:CDS
ATGTGAATTGTTGTTTGATA+AGG	0.402985	8.3:-13715160	MS.gene61452:CDS
TTAGGTTCTGTTGATGTTAG+TGG	0.403661	8.3:-13714598	MS.gene61452:CDS
CCAAATATCAAGAAAGTTAA+GGG	0.409701	8.3:+13715455	None:intergenic
TAATAGGTGGTTACGCGATC+CGG	0.412621	8.3:-13715064	MS.gene61452:CDS
GGCCTTACCCTCAGTGATGC+TGG	0.416985	8.3:-13715774	MS.gene61452:CDS
ACTGTTTGTCGAGGAATTAA+GGG	0.420044	8.3:+13714775	None:intergenic
GCTGAGCCGGCTTTGGCGTC+CGG	0.421714	8.3:-13715640	MS.gene61452:CDS
TGGGTACTGCTGCAAATAAT+AGG	0.424112	8.3:-13715080	MS.gene61452:CDS
GTACCCACTTTGCGAGGATC+AGG	0.425432	8.3:+13715096	None:intergenic
ATAGCAATGTTGTCGGCGTT+TGG	0.425654	8.3:+13715732	None:intergenic
TTGATGATGGAGTGCGAGGT+TGG	0.427732	8.3:-13715134	MS.gene61452:CDS
ATCGTACACCCACGGTCCTT+TGG	0.429955	8.3:+13715533	None:intergenic
TGCAGGTACATCAACTTTGC+TGG	0.439086	8.3:-13714833	MS.gene61452:CDS
CTATGCAAGTGGATGAATTA+GGG	0.444092	8.3:-13714636	MS.gene61452:CDS
ACCATGGAAATCAATCTTGA+TGG	0.452062	8.3:-13715795	MS.gene61452:CDS
CGCATCACCGGGAGCTTCAA+GGG	0.455331	8.3:+13714747	None:intergenic
TTTGATAAGGATGTTGATGA+TGG	0.456262	8.3:-13715147	MS.gene61452:CDS
GTTAACAGCTTGCGCATCAC+CGG	0.459939	8.3:+13714735	None:intergenic
CAGACTTGAAGAAGCGTAAG+AGG	0.463326	8.3:-13714690	MS.gene61452:CDS
TATGGCGGTAACAGAAAGAT+TGG	0.466014	8.3:-13713679	MS.gene61452:CDS
GTGTCGCTTATTTGCGTGAA+AGG	0.473487	8.3:-13715662	MS.gene61452:CDS
TGGATGAATTAGGGACTGTT+GGG	0.475281	8.3:-13714627	MS.gene61452:CDS
CGGCTTTGGCGTCCGGTGAA+GGG	0.480872	8.3:-13715633	MS.gene61452:CDS
GACATCTTTCGCAACCATAC+CGG	0.484033	8.3:+13715479	None:intergenic
AGGTTCTGTTGATGTTAGTG+GGG	0.484352	8.3:-13714596	MS.gene61452:CDS
GTGGATGAATTAGGGACTGT+TGG	0.485533	8.3:-13714628	MS.gene61452:CDS
TGACCACTGCTTAGTTGGGA+GGG	0.493364	8.3:-13715706	MS.gene61452:CDS
CACGGACCTCCTTGTCAGTA+AGG	0.498150	8.3:+13715682	None:intergenic
GATTAAATTGGAATAAGAAT+CGG	0.501509	8.3:+13715583	None:intergenic
TTCCAGCATCACTGAGGGTA+AGG	0.504961	8.3:+13715772	None:intergenic
GGTTCTGTTGATGTTAGTGG+GGG	0.506853	8.3:-13714595	MS.gene61452:CDS
GATGATGGAGTGCGAGGTTG+GGG	0.507271	8.3:-13715132	MS.gene61452:CDS
TTGACCACTGCTTAGTTGGG+AGG	0.510888	8.3:-13715707	MS.gene61452:CDS
TTTGGTTGAATAAAACCAAA+AGG	0.513707	8.3:+13715414	None:intergenic
CTGAACCTCTGAAACAAGAA+TGG	0.520329	8.3:-13715287	MS.gene61452:CDS
AACAATGAAAAGGAAAGTGT+TGG	0.524904	8.3:-13714715	MS.gene61452:CDS
CAATGGTAGTTCTAGGAGGA+AGG	0.528927	8.3:-13714939	MS.gene61452:CDS
CAGAAGGGAAGGAGGTGAGT+GGG	0.531303	8.3:-13715259	MS.gene61452:CDS
GCAAGCTGTTAACAATGAAA+AGG	0.531518	8.3:-13714725	MS.gene61452:CDS
ATACGCCATTCTTGTTTCAG+AGG	0.535300	8.3:+13715282	None:intergenic
CCAGAAAAGAACAATACTGC+TGG	0.535785	8.3:-13714799	MS.gene61452:CDS
GTCAAGAATAGCAATGTTGT+CGG	0.541420	8.3:+13715725	None:intergenic
ATTTGCGTGAAAGGCTGAGC+CGG	0.541453	8.3:-13715653	MS.gene61452:CDS
GCCATCAAGATTGATTTCCA+TGG	0.544570	8.3:+13715794	None:intergenic
GGAACGGATTCTACCCGGTA+TGG	0.544991	8.3:-13715493	MS.gene61452:CDS
CATGCTATTGCTCAACATCT+GGG	0.545756	8.3:-13715384	MS.gene61452:CDS
TTAACAGCTTGCGCATCACC+GGG	0.547454	8.3:+13714736	None:intergenic
GAACCAACATCAGCCTTCAC+CGG	0.547975	8.3:+13715045	None:intergenic
CCTTCACCGGACGCCAAAGC+CGG	0.551679	8.3:+13715634	None:intergenic
GTGCTTGCCCTTGAAGCTCC+CGG	0.553011	8.3:-13714754	MS.gene61452:CDS
AAGTTCACAGGCGAATTCAA+TGG	0.553234	8.3:-13714956	MS.gene61452:CDS
AACAAGAATGGCGTATCAGA+AGG	0.553301	8.3:-13715275	MS.gene61452:CDS
GTGGTTACGCGATCCGGTGA+AGG	0.556779	8.3:-13715058	MS.gene61452:CDS
AACATTATACTAAATGCTGC+AGG	0.556954	8.3:-13714850	MS.gene61452:CDS
ACATCTTTCGCAACCATACC+GGG	0.558074	8.3:+13715480	None:intergenic
TAAAACCAAAAGGCAGATTG+TGG	0.562828	8.3:+13715424	None:intergenic
AGTATTTGCTGAAAGTTCAC+AGG	0.565092	8.3:-13714968	MS.gene61452:CDS
CTTGAAGAAGCGTAAGAGGA+AGG	0.566229	8.3:-13714686	MS.gene61452:CDS
CAGTTCGTTCATGAAGCTGA+AGG	0.577794	8.3:-13715325	MS.gene61452:CDS
GTTCTGTTGATGTTAGTGGG+GGG	0.581760	8.3:-13714594	MS.gene61452:CDS
GCAGCAGTACCCACTTTGCG+AGG	0.595311	8.3:+13715090	None:intergenic
GATCCGGTGAAGGCTGATGT+TGG	0.596783	8.3:-13715048	MS.gene61452:CDS
AAGCCTGATCCTCGCAAAGT+GGG	0.597152	8.3:-13715099	MS.gene61452:CDS
GAATGGCGTATCAGAAGGGA+AGG	0.598254	8.3:-13715270	MS.gene61452:CDS
CGATGGCTGTAACATGGTGG+TGG	0.604677	8.3:-13715514	MS.gene61452:CDS
AAGAATCGGTCATCAAGTGT+CGG	0.607513	8.3:+13715597	None:intergenic
TAAGCCTGATCCTCGCAAAG+TGG	0.608732	8.3:-13715100	MS.gene61452:CDS
TCAGAAGGGAAGGAGGTGAG+TGG	0.617338	8.3:-13715260	MS.gene61452:CDS
GTGGTGGAACGGATTCTACC+CGG	0.621201	8.3:-13715498	MS.gene61452:CDS
ACAAGAATGGCGTATCAGAA+GGG	0.621468	8.3:-13715274	MS.gene61452:CDS
AGTATAGTAACTCCCTTCAC+CGG	0.622862	8.3:+13715621	None:intergenic
TAATGTTGTACAAGAGAGTG+TGG	0.626992	8.3:-13714659	MS.gene61452:CDS
CGTGATTCCAGCATCACTGA+GGG	0.627625	8.3:+13715767	None:intergenic
AGGGCAAGCACTGTTTGTCG+AGG	0.628864	8.3:+13714766	None:intergenic
GCTGAAAATGTATTGACCAA+AGG	0.631150	8.3:-13715549	MS.gene61452:CDS
GGTTTCTAGTGCAGGTATGG+CGG	0.632581	8.3:-13713694	MS.gene61452:intron
CAAATATCAAGAAAGTTAAG+GGG	0.634262	8.3:+13715456	None:intergenic
TGCTGGAATCACGATAAACA+TGG	0.637768	8.3:-13715757	MS.gene61452:CDS
ATGTATTGACCAAAGGACCG+TGG	0.638932	8.3:-13715542	MS.gene61452:CDS
GCTGTAACATGGTGGTGGAA+CGG	0.639798	8.3:-13715509	MS.gene61452:CDS
AGAGAGTGTGGCTATGCAAG+TGG	0.641651	8.3:-13714647	MS.gene61452:CDS
TGTATTGACCAAAGGACCGT+GGG	0.644547	8.3:-13715541	MS.gene61452:CDS
GGTGTACGATGGCTGTAACA+TGG	0.646268	8.3:-13715520	MS.gene61452:CDS
TGGCGTATCAGAAGGGAAGG+AGG	0.654691	8.3:-13715267	MS.gene61452:CDS
TGGGAGGGTCCTTACTGACA+AGG	0.656838	8.3:-13715691	MS.gene61452:CDS
AAATATCAAGAAAGTTAAGG+GGG	0.671086	8.3:+13715457	None:intergenic
TTACAGCCATCGTACACCCA+CGG	0.679543	8.3:+13715525	None:intergenic
GATGTTGATGATGGAGTGCG+AGG	0.679922	8.3:-13715138	MS.gene61452:CDS
GGACCCTCCCAACTAAGCAG+TGG	0.680574	8.3:+13715703	None:intergenic
AAAGGACCGTGGGTGTACGA+TGG	0.685131	8.3:-13715531	MS.gene61452:CDS
AATTCAATGGTAGTTCTAGG+AGG	0.688254	8.3:-13714943	MS.gene61452:CDS
GTACTGCTGCAAATAATAGG+TGG	0.695150	8.3:-13715077	MS.gene61452:CDS
TCGTGATTCCAGCATCACTG+AGG	0.695246	8.3:+13715766	None:intergenic
GAGGGTCCTTACTGACAAGG+AGG	0.712791	8.3:-13715688	MS.gene61452:CDS
GTACGATGGCTGTAACATGG+TGG	0.739222	8.3:-13715517	MS.gene61452:CDS
TTTCACGCAAATAAGCGACA+CGG	0.755535	8.3:+13715664	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AGTTCCAAAATTAAAATATT+TGG	-	chr8.3:13715017-13715036	MS.gene61452:CDS	15.0%
!!!	TCAAATTTAAAAGTTCTTTT+AGG	+	chr8.3:13715690-13715709	None:intergenic	15.0%
!!	GATTAAATTGGAATAAGAAT+CGG	+	chr8.3:13713875-13713894	None:intergenic	20.0%
!!!	CGAACCAAATATTTTAATTT+TGG	+	chr8.3:13715024-13715043	None:intergenic	20.0%
!!!	TTGATTGATATTTTTATGGA+AGG	-	chr8.3:13715477-13715496	MS.gene61452:CDS	20.0%
!	AAATATCAAGAAAGTTAAGG+GGG	+	chr8.3:13714001-13714020	None:intergenic	25.0%
!	AACAGACATATCAAACTTTA+TGG	+	chr8.3:13714570-13714589	None:intergenic	25.0%
!	ACATCATGTTCATCAAATAT+AGG	+	chr8.3:13714914-13714933	None:intergenic	25.0%
!	ATGATATTGTCTCAAATTGT+TGG	-	chr8.3:13715739-13715758	MS.gene61452:CDS	25.0%
!	ATTTGATGAACATGATGTAA+CGG	-	chr8.3:13714916-13714935	MS.gene61452:CDS	25.0%
!	CAAATATCAAGAAAGTTAAG+GGG	+	chr8.3:13714002-13714021	None:intergenic	25.0%
!	CCAAATATCAAGAAAGTTAA+GGG	+	chr8.3:13714003-13714022	None:intergenic	25.0%
!	CCTTAACTTTCTTGATATTT+GGG	-	chr8.3:13714001-13714020	MS.gene61452:intron	25.0%
!	CTAATAATATTTCCTTACCT+TGG	-	chr8.3:13715343-13715362	MS.gene61452:CDS	25.0%
!	TGATATTGTCTCAAATTGTT+GGG	-	chr8.3:13715740-13715759	MS.gene61452:CDS	25.0%
!	TTTACATGGTTCAAAAGTTT+AGG	-	chr8.3:13715507-13715526	MS.gene61452:CDS	25.0%
!	TTTGGTTGAATAAAACCAAA+AGG	+	chr8.3:13714044-13714063	None:intergenic	25.0%
!!	AAACTTTTGAACCATGTAAA+AGG	+	chr8.3:13715507-13715526	None:intergenic	25.0%
!!	AACAATTTGAGACAATATCA+TGG	+	chr8.3:13715740-13715759	None:intergenic	25.0%
!!	CTTTTAAATTTGAAAATGCG+TGG	-	chr8.3:13715697-13715716	MS.gene61452:CDS	25.0%
!!	TCATGATTAACACATTTTGT+TGG	+	chr8.3:13714464-13714483	None:intergenic	25.0%
!!!	ATAAGTAGAGTTTTTAGCAT+CGG	+	chr8.3:13714438-13714457	None:intergenic	25.0%
!!!	ATGTGAATTGTTGTTTGATA+AGG	-	chr8.3:13714295-13714314	MS.gene61452:intron	25.0%
!!!	CATTGACTTCAACATTTATA+TGG	+	chr8.3:13715230-13715249	None:intergenic	25.0%
!!!	GTTTTTAGATATGAAAGTCT+GGG	-	chr8.3:13714227-13714246	MS.gene61452:intron	25.0%
!!!	TGGGTTGATTGATATTTTTA+TGG	-	chr8.3:13715473-13715492	MS.gene61452:CDS	25.0%
!!!	TGTTTTGCTGTAAATAGTAA+TGG	-	chr8.3:13715135-13715154	MS.gene61452:CDS	25.0%
	AACATTATACTAAATGCTGC+AGG	-	chr8.3:13714605-13714624	MS.gene61452:CDS	30.0%
	CATCAAGTCTGTGATTAAAT+TGG	+	chr8.3:13713887-13713906	None:intergenic	30.0%
	CCCAAATATCAAGAAAGTTA+AGG	+	chr8.3:13714004-13714023	None:intergenic	30.0%
	CCCTTAACTTTCTTGATATT+TGG	-	chr8.3:13714000-13714019	MS.gene61452:intron	30.0%
	CCTATAATACTCATTGTTCT+CGG	+	chr8.3:13714964-13714983	None:intergenic	30.0%
	GAACAATGAGTATTATAGGA+TGG	-	chr8.3:13714965-13714984	MS.gene61452:CDS	30.0%
!	AACAATGAAAAGGAAAGTGT+TGG	-	chr8.3:13714740-13714759	MS.gene61452:CDS	30.0%
!	TTTGATAAGGATGTTGATGA+TGG	-	chr8.3:13714308-13714327	MS.gene61452:intron	30.0%
!!	GCGCAATTCTTTTAATTGTA+AGG	-	chr8.3:13715185-13715204	MS.gene61452:CDS	30.0%
!!	GGTTTTTAGATATGAAAGTC+TGG	-	chr8.3:13714226-13714245	MS.gene61452:intron	30.0%
!!!	GGTTTTATTCAACCAAAAGT+CGG	-	chr8.3:13714047-13714066	MS.gene61452:intron	30.0%
	ACTATCCGATACTCCTTAAT+AGG	-	chr8.3:13715640-13715659	MS.gene61452:CDS	35.0%
	ACTGTTTGTCGAGGAATTAA+GGG	+	chr8.3:13714683-13714702	None:intergenic	35.0%
	AGGTGACTTTAATGACATTC+TGG	-	chr8.3:13715374-13715393	MS.gene61452:CDS	35.0%
	ATAATACTCATTGTTCTCGG+CGG	+	chr8.3:13714961-13714980	None:intergenic	35.0%
	ATGTCTGTTGCTGAAAAAAG+TGG	-	chr8.3:13714581-13714600	MS.gene61452:CDS	35.0%
	CAAAGTCTCACTAAGGAAAA+GGG	+	chr8.3:13715064-13715083	None:intergenic	35.0%
	CCGAGAACAATGAGTATTAT+AGG	-	chr8.3:13714961-13714980	MS.gene61452:CDS	35.0%
	CTATCCGATACTCCTTAATA+GGG	-	chr8.3:13715641-13715660	MS.gene61452:CDS	35.0%
	CTATGCAAGTGGATGAATTA+GGG	-	chr8.3:13714819-13714838	MS.gene61452:CDS	35.0%
	GCAAGCTGTTAACAATGAAA+AGG	-	chr8.3:13714730-13714749	MS.gene61452:CDS	35.0%
	GCTATGCTTGTGGTATATTA+GGG	-	chr8.3:13714258-13714277	MS.gene61452:intron	35.0%
	GTAAATAGTAATGGTCGAAG+TGG	-	chr8.3:13715144-13715163	MS.gene61452:CDS	35.0%
	GTCAAGAATAGCAATGTTGT+CGG	+	chr8.3:13713733-13713752	None:intergenic	35.0%
	TAAAAACCTGTAAGTTGCCA+CGG	+	chr8.3:13715267-13715286	None:intergenic	35.0%
	TAAAACCAAAAGGCAGATTG+TGG	+	chr8.3:13714034-13714053	None:intergenic	35.0%
	TAATGTTGTACAAGAGAGTG+TGG	-	chr8.3:13714796-13714815	MS.gene61452:CDS	35.0%
	TAGGTTCTGTTGATGTTAGT+GGG	-	chr8.3:13714858-13714877	MS.gene61452:CDS	35.0%
	TCAGATATTTCCAAATGCGA+AGG	-	chr8.3:13715587-13715606	MS.gene61452:CDS	35.0%
	TGCTATGCTTGTGGTATATT+AGG	-	chr8.3:13714257-13714276	MS.gene61452:intron	35.0%
	TGGATGCTAAAGAAAAACGT+GGG	-	chr8.3:13715394-13715413	MS.gene61452:CDS	35.0%
	TTAGGTTCTGTTGATGTTAG+TGG	-	chr8.3:13714857-13714876	MS.gene61452:CDS	35.0%
!	AAGTTTTCAACCTTCGCATT+TGG	+	chr8.3:13715600-13715619	None:intergenic	35.0%
!	AGTATTTGCTGAAAGTTCAC+AGG	-	chr8.3:13714487-13714506	MS.gene61452:intron	35.0%
!	GCTGAAAATGTATTGACCAA+AGG	-	chr8.3:13713906-13713925	MS.gene61452:intron	35.0%
!	TGGAAGGATATCCTTTTACA+TGG	-	chr8.3:13715493-13715512	MS.gene61452:CDS	35.0%
!	TGGCAACTTACAGGTTTTTA+CGG	-	chr8.3:13715267-13715286	MS.gene61452:CDS	35.0%
!!	AATTCAATGGTAGTTCTAGG+AGG	-	chr8.3:13714512-13714531	MS.gene61452:intron	35.0%
!!!	AAGCCTCTGTTTTATTTGAG+TGG	+	chr8.3:13715089-13715108	None:intergenic	35.0%
!!!	GTATTGTTCTTTTCTGGTTG+AGG	+	chr8.3:13714653-13714672	None:intergenic	35.0%
!!!	TTAATTTTGGAACTACGCTC+AGG	+	chr8.3:13715011-13715030	None:intergenic	35.0%
	AACAAGAATGGCGTATCAGA+AGG	-	chr8.3:13714180-13714199	MS.gene61452:intron	40.0%
	AAGAATCGGTCATCAAGTGT+CGG	+	chr8.3:13713861-13713880	None:intergenic	40.0%
	AAGTCACCTAAGATACACCA+AGG	+	chr8.3:13715363-13715382	None:intergenic	40.0%
	AAGTTCACAGGCGAATTCAA+TGG	-	chr8.3:13714499-13714518	MS.gene61452:intron	40.0%
	AATAGTAATGGTCGAAGTGG+TGG	-	chr8.3:13715147-13715166	MS.gene61452:CDS	40.0%
	ACAAGAATGGCGTATCAGAA+GGG	-	chr8.3:13714181-13714200	MS.gene61452:intron	40.0%
	ACCTAAGATACACCAAGGTA+AGG	+	chr8.3:13715358-13715377	None:intergenic	40.0%
	AGGTTCTGTTGATGTTAGTG+GGG	-	chr8.3:13714859-13714878	MS.gene61452:CDS	40.0%
	AGTATAGTAACTCCCTTCAC+CGG	+	chr8.3:13713837-13713856	None:intergenic	40.0%
	ATTCTTGACCACTGCTTAGT+TGG	-	chr8.3:13713744-13713763	MS.gene61452:intron	40.0%
	CACTGTTTGTCGAGGAATTA+AGG	+	chr8.3:13714684-13714703	None:intergenic	40.0%
	CATGCTATTGCTCAACATCT+GGG	-	chr8.3:13714071-13714090	MS.gene61452:intron	40.0%
	CCAAAGTCTCACTAAGGAAA+AGG	+	chr8.3:13715065-13715084	None:intergenic	40.0%
	CCAGAAAAGAACAATACTGC+TGG	-	chr8.3:13714656-13714675	MS.gene61452:CDS	40.0%
	CGTGCTCGATGGTTAATTAA+TGG	-	chr8.3:13715426-13715445	MS.gene61452:CDS	40.0%
	CTGGATGCTAAAGAAAAACG+TGG	-	chr8.3:13715393-13715412	MS.gene61452:CDS	40.0%
	CTTTCTTGATATTTGGGTGC+AGG	-	chr8.3:13714007-13714026	MS.gene61452:intron	40.0%
	GAGACAATATCATGGATACC+AGG	+	chr8.3:13715732-13715751	None:intergenic	40.0%
	GATGAACATGATGTAACGGT+GGG	-	chr8.3:13714920-13714939	MS.gene61452:CDS	40.0%
	GATGCTAAAGAAAAACGTGG+GGG	-	chr8.3:13715396-13715415	MS.gene61452:CDS	40.0%
	GCGAATTCAATGGTAGTTCT+AGG	-	chr8.3:13714509-13714528	MS.gene61452:intron	40.0%
	GCTATGCAAGTGGATGAATT+AGG	-	chr8.3:13714818-13714837	MS.gene61452:CDS	40.0%
	GGATGCTAAAGAAAAACGTG+GGG	-	chr8.3:13715395-13715414	MS.gene61452:CDS	40.0%
	GGTCCACTCAAATAAAACAG+AGG	-	chr8.3:13715083-13715102	MS.gene61452:CDS	40.0%
	GGTTGAAAACTTAGTAGCTC+CGG	-	chr8.3:13715608-13715627	MS.gene61452:CDS	40.0%
	GTACTGCTGCAAATAATAGG+TGG	-	chr8.3:13714378-13714397	MS.gene61452:intron	40.0%
	TAATACTCATTGTTCTCGGC+GGG	+	chr8.3:13714960-13714979	None:intergenic	40.0%
	TATGGCGGTAACAGAAAGAT+TGG	-	chr8.3:13715776-13715795	MS.gene61452:CDS	40.0%
	TCATGCTATTGCTCAACATC+TGG	-	chr8.3:13714070-13714089	MS.gene61452:intron	40.0%
	TGATGAACATGATGTAACGG+TGG	-	chr8.3:13714919-13714938	MS.gene61452:CDS	40.0%
	TGCTGGAATCACGATAAACA+TGG	-	chr8.3:13713698-13713717	MS.gene61452:intron	40.0%
	TGGATGAATTAGGGACTGTT+GGG	-	chr8.3:13714828-13714847	MS.gene61452:CDS	40.0%
	TTCTTGACCACTGCTTAGTT+GGG	-	chr8.3:13713745-13713764	MS.gene61452:intron	40.0%
	TTTCACGCAAATAAGCGACA+CGG	+	chr8.3:13713794-13713813	None:intergenic	40.0%
!	ATACGCCATTCTTGTTTCAG+AGG	+	chr8.3:13714176-13714195	None:intergenic	40.0%
!	CCAGCAGTATTGTTCTTTTC+TGG	+	chr8.3:13714659-13714678	None:intergenic	40.0%
!	CCTTTTCCTTAGTGAGACTT+TGG	-	chr8.3:13715062-13715081	MS.gene61452:CDS	40.0%
!	GAGGCTTTTCGTTATATGCT+TGG	-	chr8.3:13715102-13715121	MS.gene61452:CDS	40.0%
!	GGCAACTTACAGGTTTTTAC+GGG	-	chr8.3:13715268-13715287	MS.gene61452:CDS	40.0%
!	GTGATTCGTGGAACTTTTTG+AGG	-	chr8.3:13715313-13715332	MS.gene61452:CDS	40.0%
!	TCCTTACCTTGGTGTATCTT+AGG	-	chr8.3:13715354-13715373	MS.gene61452:CDS	40.0%
!	TGGGTACTGCTGCAAATAAT+AGG	-	chr8.3:13714375-13714394	MS.gene61452:intron	40.0%
!	TTTGGTTCGCTATTACAAGC+CGG	-	chr8.3:13715035-13715054	MS.gene61452:CDS	40.0%
!!	AATTGTTGGGTTTCTAGTGC+AGG	-	chr8.3:13715753-13715772	MS.gene61452:CDS	40.0%
!!	CTGAACCTCTGAAACAAGAA+TGG	-	chr8.3:13714168-13714187	MS.gene61452:intron	40.0%
!!	GTGGTGGTTTAGCTTTATTC+TGG	-	chr8.3:13715163-13715182	MS.gene61452:CDS	40.0%
	ACATCTTTCGCAACCATACC+GGG	+	chr8.3:13713978-13713997	None:intergenic	45.0%
	AGTGGACCAAAGTCTCACTA+AGG	+	chr8.3:13715071-13715090	None:intergenic	45.0%
	ATAGCAATGTTGTCGGCGTT+TGG	+	chr8.3:13713726-13713745	None:intergenic	45.0%
	ATCGGATAGTGATCAGAAGC+CGG	+	chr8.3:13715630-13715649	None:intergenic	45.0%
	ATTATAGGATGGAACTGCCG+AGG	-	chr8.3:13714976-13714995	MS.gene61452:CDS	45.0%
	CAGTTCGTTCATGAAGCTGA+AGG	-	chr8.3:13714130-13714149	MS.gene61452:intron	45.0%
	GAAGTCAATGATCCTAGTCG+AGG	-	chr8.3:13715240-13715259	MS.gene61452:CDS	45.0%
	GACATCTTTCGCAACCATAC+CGG	+	chr8.3:13713979-13713998	None:intergenic	45.0%
	GGTTCTGTTGATGTTAGTGG+GGG	-	chr8.3:13714860-13714879	MS.gene61452:CDS	45.0%
	GTGGATGAATTAGGGACTGT+TGG	-	chr8.3:13714827-13714846	MS.gene61452:CDS	45.0%
	GTTCTGTTGATGTTAGTGGG+GGG	-	chr8.3:13714861-13714880	MS.gene61452:CDS	45.0%
	TAATAGGTGGTTACGCGATC+CGG	-	chr8.3:13714391-13714410	MS.gene61452:intron	45.0%
	TGCAGGTACATCAACTTTGC+TGG	-	chr8.3:13714622-13714641	MS.gene61452:CDS	45.0%
!	ATGTATTGACCAAAGGACCG+TGG	-	chr8.3:13713913-13713932	MS.gene61452:intron	45.0%
!	CAGACTTGAAGAAGCGTAAG+AGG	-	chr8.3:13714765-13714784	MS.gene61452:CDS	45.0%
!	CTTGAAGAAGCGTAAGAGGA+AGG	-	chr8.3:13714769-13714788	MS.gene61452:CDS	45.0%
!	GGCTCCCTATTAAGGAGTAT+CGG	+	chr8.3:13715648-13715667	None:intergenic	45.0%
!	GGGACTGTTGGGATTGATTT+AGG	-	chr8.3:13714839-13714858	MS.gene61452:CDS	45.0%
!	TGTATTGACCAAAGGACCGT+GGG	-	chr8.3:13713914-13713933	MS.gene61452:intron	45.0%
!!	CAATGGTAGTTCTAGGAGGA+AGG	-	chr8.3:13714516-13714535	MS.gene61452:intron	45.0%
!!	GCAATAGCATGACCGACTTT+TGG	+	chr8.3:13714062-13714081	None:intergenic	45.0%
!!	GGTCTGTTTTGCTATGCTTG+TGG	-	chr8.3:13714248-13714267	MS.gene61452:intron	45.0%
!!	GTCAAGCTGTTTCTGATGCT+GGG	-	chr8.3:13715454-13715473	MS.gene61452:CDS	45.0%
!!	GTGTCGCTTATTTGCGTGAA+AGG	-	chr8.3:13713793-13713812	MS.gene61452:intron	45.0%
!!	TTGGGTTTCTAGTGCAGGTA+TGG	-	chr8.3:13715758-13715777	MS.gene61452:CDS	45.0%
	AAGCCTGATCCTCGCAAAGT+GGG	-	chr8.3:13714356-13714375	MS.gene61452:intron	50.0%
	AATGCGTGGTGTGTTGAACC+TGG	-	chr8.3:13715711-13715730	MS.gene61452:CDS	50.0%
	AGGAGGTGAGTGGGTAACTA+TGG	-	chr8.3:13714205-13714224	MS.gene61452:intron	50.0%
	ATCACGCCTTCTACCTCCTT+CGG	+	chr8.3:13715298-13715317	None:intergenic	50.0%
	ATTTGCGTGAAAGGCTGAGC+CGG	-	chr8.3:13713802-13713821	MS.gene61452:intron	50.0%
	CGTGATTCCAGCATCACTGA+GGG	+	chr8.3:13713691-13713710	None:intergenic	50.0%
	CTTTGCGAGGATCAGGCTTA+AGG	+	chr8.3:13714355-13714374	None:intergenic	50.0%
	GAACCAACATCAGCCTTCAC+CGG	+	chr8.3:13714413-13714432	None:intergenic	50.0%
	GAATGGCGTATCAGAAGGGA+AGG	-	chr8.3:13714185-13714204	MS.gene61452:intron	50.0%
	GATGGAACTGCCGAGGTTTA+GGG	-	chr8.3:13714983-13715002	MS.gene61452:CDS	50.0%
	GGAGCCTGCTACACGAATTT+GGG	-	chr8.3:13715662-13715681	MS.gene61452:CDS	50.0%
	GGTGTACGATGGCTGTAACA+TGG	-	chr8.3:13713935-13713954	MS.gene61452:intron	50.0%
	GTACGATGGCTGTAACATGG+TGG	-	chr8.3:13713938-13713957	MS.gene61452:intron	50.0%
	GTGTAGCAGGCTCCCTATTA+AGG	+	chr8.3:13715656-13715675	None:intergenic	50.0%
	GTTAACAGCTTGCGCATCAC+CGG	+	chr8.3:13714723-13714742	None:intergenic	50.0%
	TAAGCCTGATCCTCGCAAAG+TGG	-	chr8.3:13714355-13714374	MS.gene61452:intron	50.0%
	TCGTGATTCCAGCATCACTG+AGG	+	chr8.3:13713692-13713711	None:intergenic	50.0%
	TGACCACTGCTTAGTTGGGA+GGG	-	chr8.3:13713749-13713768	MS.gene61452:intron	50.0%
	TTAACAGCTTGCGCATCACC+GGG	+	chr8.3:13714722-13714741	None:intergenic	50.0%
	TTACAGCCATCGTACACCCA+CGG	+	chr8.3:13713933-13713952	None:intergenic	50.0%
	TTCCAGCATCACTGAGGGTA+AGG	+	chr8.3:13713686-13713705	None:intergenic	50.0%
	TTGACCACTGCTTAGTTGGG+AGG	-	chr8.3:13713748-13713767	MS.gene61452:intron	50.0%
	TTTAGGTACCCCTAGAGCTG+TGG	-	chr8.3:13715524-13715543	MS.gene61452:CDS	50.0%
!	AGAGAGTGTGGCTATGCAAG+TGG	-	chr8.3:13714808-13714827	MS.gene61452:CDS	50.0%
!	CAGGTCCACAATCTGCCTTT+TGG	-	chr8.3:13714026-13714045	MS.gene61452:intron	50.0%
!	GGTTTTTACGGGTTTCCCGA+AGG	-	chr8.3:13715279-13715298	MS.gene61452:CDS	50.0%
!	TTTTACGGGTTTCCCGAAGG+AGG	-	chr8.3:13715282-13715301	MS.gene61452:CDS	50.0%
!!	CGTCAAGCTGTTTCTGATGC+TGG	-	chr8.3:13715453-13715472	MS.gene61452:CDS	50.0%
!!	GATGTTGATGATGGAGTGCG+AGG	-	chr8.3:13714317-13714336	MS.gene61452:intron	50.0%
!!	GCTGTAACATGGTGGTGGAA+CGG	-	chr8.3:13713946-13713965	MS.gene61452:intron	50.0%
!!	GGTTTCTAGTGCAGGTATGG+CGG	-	chr8.3:13715761-13715780	MS.gene61452:CDS	50.0%
!!	TCACTAAGGAAAAGGGCGTC+CGG	+	chr8.3:13715057-13715076	None:intergenic	50.0%
!!	TGATGATGGAGTGCGAGGTT+GGG	-	chr8.3:13714322-13714341	MS.gene61452:intron	50.0%
!!	TTGATGATGGAGTGCGAGGT+TGG	-	chr8.3:13714321-13714340	MS.gene61452:intron	50.0%
	AAAGGACCGTGGGTGTACGA+TGG	-	chr8.3:13713924-13713943	MS.gene61452:intron	55.0%
	AACGGTGGGTCCTGTTAGTC+AGG	-	chr8.3:13714934-13714953	MS.gene61452:CDS	55.0%
	AAGATGCATTCGCGAGCGCA+CGG	+	chr8.3:13715563-13715582	None:intergenic	55.0%
	ATCGTACACCCACGGTCCTT+TGG	+	chr8.3:13713925-13713944	None:intergenic	55.0%
	ATGATCCTAGTCGAGGACCG+TGG	-	chr8.3:13715247-13715266	MS.gene61452:CDS	55.0%
	CACGGACCTCCTTGTCAGTA+AGG	+	chr8.3:13713776-13713795	None:intergenic	55.0%
	CAGAAGGGAAGGAGGTGAGT+GGG	-	chr8.3:13714196-13714215	MS.gene61452:intron	55.0%
	CGATGGCTGTAACATGGTGG+TGG	-	chr8.3:13713941-13713960	MS.gene61452:intron	55.0%
	GAGGGTCCTTACTGACAAGG+AGG	-	chr8.3:13713767-13713786	MS.gene61452:intron	55.0%
	GATCCGGTGAAGGCTGATGT+TGG	-	chr8.3:13714407-13714426	MS.gene61452:intron	55.0%
	GGATGGAACTGCCGAGGTTT+AGG	-	chr8.3:13714982-13715001	MS.gene61452:CDS	55.0%
	GGCACCCAAATTCGTGTAGC+AGG	+	chr8.3:13715669-13715688	None:intergenic	55.0%
	GGGAGCCTGCTACACGAATT+TGG	-	chr8.3:13715661-13715680	MS.gene61452:CDS	55.0%
	GGTTTCCCGAAGGAGGTAGA+AGG	-	chr8.3:13715289-13715308	MS.gene61452:CDS	55.0%
	GTACCCACTTTGCGAGGATC+AGG	+	chr8.3:13714362-13714381	None:intergenic	55.0%
	TCACGCCTTCTACCTCCTTC+GGG	+	chr8.3:13715297-13715316	None:intergenic	55.0%
	TCAGAAGGGAAGGAGGTGAG+TGG	-	chr8.3:13714195-13714214	MS.gene61452:intron	55.0%
	TGGCGTATCAGAAGGGAAGG+AGG	-	chr8.3:13714188-13714207	MS.gene61452:intron	55.0%
	TGGGAGGGTCCTTACTGACA+AGG	-	chr8.3:13713764-13713783	MS.gene61452:intron	55.0%
!	AGGGCAAGCACTGTTTGTCG+AGG	+	chr8.3:13714692-13714711	None:intergenic	55.0%
!	GAGGTAGAAGGCGTGATTCG+TGG	-	chr8.3:13715301-13715320	MS.gene61452:CDS	55.0%
!	GGAACGGATTCTACCCGGTA+TGG	-	chr8.3:13713962-13713981	MS.gene61452:intron	55.0%
!	GTGGTGGAACGGATTCTACC+CGG	-	chr8.3:13713957-13713976	MS.gene61452:intron	55.0%
!!	GATGATGGAGTGCGAGGTTG+GGG	-	chr8.3:13714323-13714342	MS.gene61452:intron	55.0%
	AACCGCTCCTCCACAGCTCT+AGG	+	chr8.3:13715537-13715556	None:intergenic	60.0%
	ACCGCTCCTCCACAGCTCTA+GGG	+	chr8.3:13715536-13715555	None:intergenic	60.0%
	AGGTACCCCTAGAGCTGTGG+AGG	-	chr8.3:13715527-13715546	MS.gene61452:CDS	60.0%
	AGTTGCCACGGTCCTCGACT+AGG	+	chr8.3:13715255-13715274	None:intergenic	60.0%
	CGAGGACCGTGGCAACTTAC+AGG	-	chr8.3:13715258-13715277	MS.gene61452:CDS	60.0%
	CGCATCACCGGGAGCTTCAA+GGG	+	chr8.3:13714711-13714730	None:intergenic	60.0%
	CGCTCAGGTGCCCTAAACCT+CGG	+	chr8.3:13714996-13715015	None:intergenic	60.0%
	GCAGCAGTACCCACTTTGCG+AGG	+	chr8.3:13714368-13714387	None:intergenic	60.0%
	GGACCCTCCCAACTAAGCAG+TGG	+	chr8.3:13713755-13713774	None:intergenic	60.0%
	GGCCTTACCCTCAGTGATGC+TGG	-	chr8.3:13713681-13713700	MS.gene61452:CDS	60.0%
	GGGGTTCTGTTCGTGCTCGA+TGG	-	chr8.3:13715415-13715434	MS.gene61452:CDS	60.0%
	GTGCGCTCGCGAATGCATCT+TGG	-	chr8.3:13715562-13715581	MS.gene61452:CDS	60.0%
	GTGCTTGCCCTTGAAGCTCC+CGG	-	chr8.3:13714701-13714720	MS.gene61452:CDS	60.0%
	GTGGTTACGCGATCCGGTGA+AGG	-	chr8.3:13714397-13714416	MS.gene61452:intron	60.0%
!	GTGAAAGGCTGAGCCGGCTT+TGG	-	chr8.3:13713808-13713827	MS.gene61452:intron	60.0%
	CCCCTAGAGCTGTGGAGGAG+CGG	-	chr8.3:13715532-13715551	MS.gene61452:CDS	65.0%
	CCTTCACCGGACGCCAAAGC+CGG	+	chr8.3:13713824-13713843	None:intergenic	65.0%
	GCGCATCACCGGGAGCTTCA+AGG	+	chr8.3:13714712-13714731	None:intergenic	65.0%
	TCTCGGCGGGCCTGACTAAC+AGG	+	chr8.3:13714947-13714966	None:intergenic	65.0%
!	CCGCTCCTCCACAGCTCTAG+GGG	+	chr8.3:13715535-13715554	None:intergenic	65.0%
!	CGGCTTTGGCGTCCGGTGAA+GGG	-	chr8.3:13713822-13713841	MS.gene61452:intron	65.0%
!	CCGGCTTTGGCGTCCGGTGA+AGG	-	chr8.3:13713821-13713840	MS.gene61452:intron	70.0%
!	GCTGAGCCGGCTTTGGCGTC+CGG	-	chr8.3:13713815-13713834	MS.gene61452:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	13713663	13715814	13713663	ID=MS.gene61452
chr8.3	mRNA	13713663	13715814	13713663	ID=MS.gene61452.t1;Parent=MS.gene61452
chr8.3	exon	13714574	13715814	13714574	ID=MS.gene61452.t1.exon1;Parent=MS.gene61452.t1
chr8.3	CDS	13714574	13715814	13714574	ID=cds.MS.gene61452.t1;Parent=MS.gene61452.t1
chr8.3	exon	13713663	13713702	13713663	ID=MS.gene61452.t1.exon2;Parent=MS.gene61452.t1
chr8.3	CDS	13713663	13713702	13713663	ID=cds.MS.gene61452.t1;Parent=MS.gene61452.t1

Gene Sequence

>MS.gene61452

ATGGAAATCAATCTTGATGGCCTTACCCTCAGTGATGCTGGAATCACGATAAACATGGAACCAAACGCCGACAACATTGCTATTCTTGACCACTGCTTAGTTGGGAGGGTCCTTACTGACAAGGAGGTCCGTGTCGCTTATTTGCGTGAAAGGCTGAGCCGGCTTTGGCGTCCGGTGAAGGGAGTTACTATACTTCCGACACTTGATGACCGATTCTTATTCCAATTTAATCACAGACTTGATGCTGAAAATGTATTGACCAAAGGACCGTGGGTGTACGATGGCTGTAACATGGTGGTGGAACGGATTCTACCCGGTATGGTTGCGAAAGATGTCCCCCTTAACTTTCTTGATATTTGGGTGCAGGTCCACAATCTGCCTTTTGGTTTTATTCAACCAAAAGTCGGTCATGCTATTGCTCAACATCTGGGAGAGTTCAAAGAATATGATAGACTCAACACGCTGCACAGTTCGTTCATGAAGCTGAAGGTGAGAGTCAATGTGTCTGAACCTCTGAAACAAGAATGGCGTATCAGAAGGGAAGGAGGTGAGTGGGTAACTATGGTTTTTAGATATGAAAGTCTGGGTCTGTTTTGCTATGCTTGTGGTATATTAGGGCATTCAGACAGAACATGTGAATTGTTGTTTGATAAGGATGTTGATGATGGAGTGCGAGGTTGGGGTGCTAACCTTAAGCCTGATCCTCGCAAAGTGGGTACTGCTGCAAATAATAGGTGGTTACGCGATCCGGTGAAGGCTGATGTTGGTTCTTCCGATGCTAAAAACTCTACTTATACACCAACAAAATGTGTTAATCATGAAGAAGTATTTGCTGAAAGTTCACAGGCGAATTCAATGGTAGTTCTAGGAGGAAGGATTAATGCTGTACACAAAGAAATCAGTTCCATAAAGTTTGATATGTCTGTTGCTGAAAAAAGTGGAAACATTATACTAAATGCTGCAGGTACATCAACTTTGCTGGAATTGCCTCAACCAGAAAAGAACAATACTGCTGGACCCTTAATTCCTCGACAAACAGTGCTTGCCCTTGAAGCTCCCGGTGATGCGCAAGCTGTTAACAATGAAAAGGAAAGTGTTGGTTCAGACTTGAAGAAGCGTAAGAGGAAGGTTAATAATGTTGTACAAGAGAGTGTGGCTATGCAAGTGGATGAATTAGGGACTGTTGGGATTGATTTAGGTTCTGTTGATGTTAGTGGGGGGAATAGTGTTATGAAAATTAAGTCTAATCCTATATTTGATGAACATGATGTAACGGTGGGTCCTGTTAGTCAGGCCCGCCGAGAACAATGAGTATTATAGGATGGAACTGCCGAGGTTTAGGGCACCTGAGCGTAGTTCCAAAATTAAAATATTTGGTTCGCTATTACAAGCCGGACGCCCTTTTCCTTAGTGAGACTTTGGTCCACTCAAATAAAACAGAGGCTTTTCGTTATATGCTTGGTTTTGAAAATTGTTTTGCTGTAAATAGTAATGGTCGAAGTGGTGGTTTAGCTTTATTCTGGCGCAATTCTTTTAATTGTAAGGTGCTGAATTTTTCTTCTAACCATATAAATGTTGAAGTCAATGATCCTAGTCGAGGACCGTGGCAACTTACAGGTTTTTACGGGTTTCCCGAAGGAGGTAGAAGGCGTGATTCGTGGAACTTTTTGAGGAATCTGTCTAATAATATTTCCTTACCTTGGTGTATCTTAGGTGACTTTAATGACATTCTGGATGCTAAAGAAAAACGTGGGGGTTCTGTTCGTGCTCGATGGTTAATTAATGGCTTTCGTCAAGCTGTTTCTGATGCTGGGTTGATTGATATTTTTATGGAAGGATATCCTTTTACATGGTTCAAAAGTTTAGGTACCCCTAGAGCTGTGGAGGAGCGGTTAGACCGTGCGCTCGCGAATGCATCTTGGCTTCAGATATTTCCAAATGCGAAGGTTGAAAACTTAGTAGCTCCGGCTTCTGATCACTATCCGATACTCCTTAATAGGGAGCCTGCTACACGAATTTGGGTGCCTAAAAGAACTTTTAAATTTGAAAATGCGTGGTGTGTTGAACCTGGTATCCATGATATTGTCTCAAATTGTTGGGTTTCTAGTGCAGGTATGGCGGTAACAGAAAGATTGGAGCATTGTGCCTCTGA

Protein sequence

>MS.gene61452.t1

MEINLDGLTLSDAGITINMEPNADNIAILDHCLVGRVLTDKEVRVAYLRERLSRLWRPVKGVTILPTLDDRFLFQFNHRLDAENVLTKGPWVYDGCNMVVERILPGMVAKDVPLNFLDIWVQVHNLPFGFIQPKVGHAIAQHLGEFKEYDRLNTLHSSFMKLKVRVNVSEPLKQEWRIRREGGEWVTMVFRYESLGLFCYACGILGHSDRTCELLFDKDVDDGVRGWGANLKPDPRKVGTAANNRWLRDPVKADVGSSDAKNSTYTPTKCVNHEEVFAESSQANSMVVLGGRINAVHKEISSIKFDMSVAEKSGNIILNAAGTSTLLELPQPEKNNTAGPLIPRQTVLALEAPGDAQAVNNEKESVGSDLKKRKRKVNNVVQESVAMQVDELGTVGIDLGSVDVSGGNSVMKIKYGGNRKIGALCL