AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene66650


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene66650.t1 MTR_3g053920 48.971 243 58 3 1 243 103 279 9.23e-62 196
MS.gene66650.t1 MTR_4g103530 86.957 46 6 0 1 46 49 94 1.49e-22 92.0
MS.gene66650.t1 MTR_6g033215 36.458 96 59 1 4 97 199 294 2.56e-12 65.9
MS.gene66650.t1 MTR_0010s0370 44.928 69 34 2 10 75 110 177 9.01e-11 60.1
MS.gene66650.t1 MTR_0010s0350 44.928 69 34 2 10 75 110 177 9.01e-11 60.1
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 58 sgRNAs with CRISPR-Local

Find 69 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTTTAGCTAGCACCGCATTC+TGG 0.141973 4.3:-48940888 None:intergenic
AGAAGCCAGCAGATGTATTT+TGG 0.145079 4.3:-48940935 None:intergenic
CCAAGTCATCCTTTGGGGCT+TGG 0.235303 4.3:+48940991 MS.gene66650:CDS
GTTCCAAACCTTTGCGTAAC+TGG 0.267932 4.3:-48940676 None:intergenic
CCTATGCCTTGACCTACTTT+TGG 0.278124 4.3:-48940376 None:intergenic
CCTGCTTCCAAGTCATCCTT+TGG 0.318496 4.3:+48940984 MS.gene66650:CDS
CTGATGATGGCGTCCGCAAT+TGG 0.330887 4.3:+48940638 MS.gene66650:CDS
CTGCTTCCAAGTCATCCTTT+GGG 0.362390 4.3:+48940985 MS.gene66650:CDS
CAAGGCATAGGAAGCTTCTT+AGG 0.376769 4.3:+48940388 MS.gene66650:CDS
CAACCAACGGTTTGTGGCAG+CGG 0.377733 4.3:-48940698 None:intergenic
CAAGTTCACGGTCTGCCTTT+CGG 0.389727 4.3:+48940343 MS.gene66650:CDS
TGATGATGGCGTCCGCAATT+GGG 0.395960 4.3:+48940639 MS.gene66650:CDS
TTTAGCTAGCACCGCATTCT+GGG 0.402038 4.3:-48940887 None:intergenic
AGCGACTCTGACACACATAT+TGG 0.405483 4.3:-48940452 None:intergenic
AAACCAGTTACGCAAAGGTT+TGG 0.415112 4.3:+48940673 MS.gene66650:CDS
TTCGAATTAGATTCTGATGA+TGG 0.422237 4.3:+48940625 MS.gene66650:CDS
TGTCACAGGTGTGGATTGAT+TGG 0.423731 4.3:+48940574 MS.gene66650:CDS
TGCTTCCAAAATACATCTGC+TGG 0.424718 4.3:+48940930 MS.gene66650:CDS
TGGTTCAGTGGGACAAAGCT+AGG 0.443161 4.3:-48940304 None:intergenic
ATCTTGCAACCAACGGTTTG+TGG 0.450416 4.3:-48940704 None:intergenic
CCAAGCCCCAAAGGATGACT+TGG 0.469128 4.3:-48940991 None:intergenic
ACCATGGATCTTATCGCTCC+TGG 0.475299 4.3:+48940274 None:intergenic
CAAGCAGGTGCTGCTTCTGT+TGG 0.476934 4.3:+48940760 MS.gene66650:CDS
ATCTAATGATGACATACAAT+AGG 0.483060 4.3:+48941041 MS.gene66650:CDS
TGTGACATAGAAGCAGGAAT+AGG 0.487822 4.3:-48940727 None:intergenic
CCAAAGGATGACTTGGAAGC+AGG 0.490656 4.3:-48940984 None:intergenic
CAACAGAAGCAGCACCTGCT+TGG 0.492986 4.3:-48940759 None:intergenic
TCATTAGATTCACTTGAAAG+TGG 0.496760 4.3:-48941027 None:intergenic
AGTAACAAAAGCACCGTTTG+TGG 0.497986 4.3:-48940518 None:intergenic
CTGCTGGCTTCTTCTTCCAC+AGG 0.507471 4.3:+48940946 MS.gene66650:CDS
GATGATGGCGTCCGCAATTG+GGG 0.507633 4.3:+48940640 MS.gene66650:CDS
TGGCGAGTCCGTGCCACAAA+CGG 0.511841 4.3:+48940505 MS.gene66650:CDS
CACCTTCAGTAGCTGCAGTT+CGG 0.513939 4.3:-48940783 None:intergenic
TGCTTCCAAGTCATCCTTTG+GGG 0.514970 4.3:+48940986 MS.gene66650:CDS
TCACTTGAAAGTGGAGCAGC+AGG 0.515569 4.3:-48941018 None:intergenic
GGATTCATCCAACCAAAAGT+AGG 0.533518 4.3:+48940364 MS.gene66650:CDS
GCAGGTAAGCCAAGCCCCAA+AGG 0.549880 4.3:-48941000 None:intergenic
GTTTGATGTGACATAGAAGC+AGG 0.552176 4.3:-48940733 None:intergenic
TCATGTCAGCTCCCCAATTG+CGG 0.560990 4.3:-48940651 None:intergenic
GAACCGCTGCCACAAACCGT+TGG 0.569652 4.3:+48940695 MS.gene66650:CDS
AACTGCAGCTACTGAAGGTG+TGG 0.577023 4.3:+48940786 MS.gene66650:CDS
AGAATCTAATTCGAACAACT+CGG 0.580030 4.3:-48940617 None:intergenic
CAAAAGCACCGTTTGTGGCA+CGG 0.590707 4.3:-48940513 None:intergenic
GGTTCAGTGGGACAAAGCTA+GGG 0.592996 4.3:-48940303 None:intergenic
TCACATCAAACTCACCAAGC+AGG 0.593385 4.3:+48940745 MS.gene66650:CDS
GTGCTAGCTAAAGAAGGTAA+AGG 0.593767 4.3:+48940898 MS.gene66650:CDS
GAATCTAATTCGAACAACTC+GGG 0.601829 4.3:-48940616 None:intergenic
ACAAAGCTAGGGACGACACC+AGG 0.602101 4.3:-48940292 None:intergenic
AATGCGGTGCTAGCTAAAGA+AGG 0.603825 4.3:+48940892 MS.gene66650:CDS
GGCCGAACTGCAGCTACTGA+AGG 0.610869 4.3:+48940781 MS.gene66650:CDS
ATCCAACCAAAAGTAGGTCA+AGG 0.617189 4.3:+48940370 MS.gene66650:CDS
CCAAAAGTAGGTCAAGGCAT+AGG 0.620618 4.3:+48940376 MS.gene66650:CDS
ACCAGGAGCGATAAGATCCA+TGG 0.622459 4.3:-48940275 None:intergenic
GCTAGTAGCTGAAGTACCTG+TGG 0.624706 4.3:-48940962 None:intergenic
TGTGCTTGCTGCCCAGAATG+CGG 0.637409 4.3:+48940876 MS.gene66650:CDS
ACATGAAACCAGTTACGCAA+AGG 0.638258 4.3:+48940668 MS.gene66650:CDS
GAATAGGATCTTGCAACCAA+CGG 0.654985 4.3:-48940711 None:intergenic
TTTGGTTGGATGAATCCGAA+AGG 0.672828 4.3:-48940358 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACTTTAAATATGAAAAATT+GGG + chr4.3:48940544-48940563 MS.gene66650:CDS 10.0%
!! TAACTTTAAATATGAAAAAT+TGG + chr4.3:48940543-48940562 MS.gene66650:CDS 10.0%
! AAAAATCAATATGGTTCAGT+GGG - chr4.3:48940318-48940337 None:intergenic 25.0%
!!! ACTGAACCATATTGATTTTT+GGG + chr4.3:48940318-48940337 MS.gene66650:CDS 25.0%
AGAATCTAATTCGAACAACT+CGG - chr4.3:48940620-48940639 None:intergenic 30.0%
CAAAAATCAATATGGTTCAG+TGG - chr4.3:48940319-48940338 None:intergenic 30.0%
CTTGAACCCAAAAATCAATA+TGG - chr4.3:48940327-48940346 None:intergenic 30.0%
TCATTAGATTCACTTGAAAG+TGG - chr4.3:48941030-48941049 None:intergenic 30.0%
TTCGAATTAGATTCTGATGA+TGG + chr4.3:48940625-48940644 MS.gene66650:CDS 30.0%
!!! CACTGAACCATATTGATTTT+TGG + chr4.3:48940317-48940336 MS.gene66650:CDS 30.0%
GAATCTAATTCGAACAACTC+GGG - chr4.3:48940619-48940638 None:intergenic 35.0%
!!! GATTTTTGGGTTCAAGTTCA+CGG + chr4.3:48940331-48940350 MS.gene66650:CDS 35.0%
AAACCAGTTACGCAAAGGTT+TGG + chr4.3:48940673-48940692 MS.gene66650:CDS 40.0%
ACATGAAACCAGTTACGCAA+AGG + chr4.3:48940668-48940687 MS.gene66650:CDS 40.0%
ATCCAACCAAAAGTAGGTCA+AGG + chr4.3:48940370-48940389 MS.gene66650:CDS 40.0%
GAATAGGATCTTGCAACCAA+CGG - chr4.3:48940714-48940733 None:intergenic 40.0%
GGATTCATCCAACCAAAAGT+AGG + chr4.3:48940364-48940383 MS.gene66650:CDS 40.0%
GTGCTAGCTAAAGAAGGTAA+AGG + chr4.3:48940898-48940917 MS.gene66650:CDS 40.0%
GTTTGATGTGACATAGAAGC+AGG - chr4.3:48940736-48940755 None:intergenic 40.0%
TGCTTCCAAAATACATCTGC+TGG + chr4.3:48940930-48940949 MS.gene66650:CDS 40.0%
TGTGACATAGAAGCAGGAAT+AGG - chr4.3:48940730-48940749 None:intergenic 40.0%
TTTGGTTGGATGAATCCGAA+AGG - chr4.3:48940361-48940380 None:intergenic 40.0%
! AATTGGGTGTGTTTTGTCAC+AGG + chr4.3:48940560-48940579 MS.gene66650:CDS 40.0%
! AGAAGCCAGCAGATGTATTT+TGG - chr4.3:48940938-48940957 None:intergenic 40.0%
!! AGTAACAAAAGCACCGTTTG+TGG - chr4.3:48940521-48940540 None:intergenic 40.0%
AGCGACTCTGACACACATAT+TGG - chr4.3:48940455-48940474 None:intergenic 45.0%
ATCTTGCAACCAACGGTTTG+TGG - chr4.3:48940707-48940726 None:intergenic 45.0%
CAAGGCATAGGAAGCTTCTT+AGG + chr4.3:48940388-48940407 MS.gene66650:CDS 45.0%
CCAAAAGTAGGTCAAGGCAT+AGG + chr4.3:48940376-48940395 MS.gene66650:CDS 45.0%
CTGCTTCCAAGTCATCCTTT+GGG + chr4.3:48940985-48941004 MS.gene66650:CDS 45.0%
CTGTCCCACTGAAAAAAGAG+TGG + chr4.3:48940485-48940504 MS.gene66650:CDS 45.0%
GTTCCAAACCTTTGCGTAAC+TGG - chr4.3:48940679-48940698 None:intergenic 45.0%
TCACATCAAACTCACCAAGC+AGG + chr4.3:48940745-48940764 MS.gene66650:CDS 45.0%
TGCTTCCAAGTCATCCTTTG+GGG + chr4.3:48940986-48941005 MS.gene66650:CDS 45.0%
TTTAGCTAGCACCGCATTCT+GGG - chr4.3:48940890-48940909 None:intergenic 45.0%
! AATGCGGTGCTAGCTAAAGA+AGG + chr4.3:48940892-48940911 MS.gene66650:CDS 45.0%
! CCTATGCCTTGACCTACTTT+TGG - chr4.3:48940379-48940398 None:intergenic 45.0%
! TGTCACAGGTGTGGATTGAT+TGG + chr4.3:48940574-48940593 MS.gene66650:CDS 45.0%
!! TGCCTTGACCTACTTTTGGT+TGG - chr4.3:48940375-48940394 None:intergenic 45.0%
!!! ACTCGCCACTCTTTTTTCAG+TGG - chr4.3:48940493-48940512 None:intergenic 45.0%
!!! CTCGCCACTCTTTTTTCAGT+GGG - chr4.3:48940492-48940511 None:intergenic 45.0%
AACTGCAGCTACTGAAGGTG+TGG + chr4.3:48940786-48940805 MS.gene66650:CDS 50.0%
CAAGTTCACGGTCTGCCTTT+CGG + chr4.3:48940343-48940362 MS.gene66650:CDS 50.0%
CACCTTCAGTAGCTGCAGTT+CGG - chr4.3:48940786-48940805 None:intergenic 50.0%
CCTGCTTCCAAGTCATCCTT+TGG + chr4.3:48940984-48941003 MS.gene66650:CDS 50.0%
CTTTAGCTAGCACCGCATTC+TGG - chr4.3:48940891-48940910 None:intergenic 50.0%
GGTTCAGTGGGACAAAGCTA+GGG - chr4.3:48940306-48940325 None:intergenic 50.0%
TCACTTGAAAGTGGAGCAGC+AGG - chr4.3:48941021-48941040 None:intergenic 50.0%
TCATGTCAGCTCCCCAATTG+CGG - chr4.3:48940654-48940673 None:intergenic 50.0%
TGGTTCAGTGGGACAAAGCT+AGG - chr4.3:48940307-48940326 None:intergenic 50.0%
! CCAAAGGATGACTTGGAAGC+AGG - chr4.3:48940987-48941006 None:intergenic 50.0%
! GCTAGTAGCTGAAGTACCTG+TGG - chr4.3:48940965-48940984 None:intergenic 50.0%
! GGTGTGTTTTGTCACAGGTG+TGG + chr4.3:48940565-48940584 MS.gene66650:CDS 50.0%
!! CAAAAGCACCGTTTGTGGCA+CGG - chr4.3:48940516-48940535 None:intergenic 50.0%
!! TGATGATGGCGTCCGCAATT+GGG + chr4.3:48940639-48940658 MS.gene66650:CDS 50.0%
ACAAAGCTAGGGACGACACC+AGG - chr4.3:48940295-48940314 None:intergenic 55.0%
CAACAGAAGCAGCACCTGCT+TGG - chr4.3:48940762-48940781 None:intergenic 55.0%
CAACCAACGGTTTGTGGCAG+CGG - chr4.3:48940701-48940720 None:intergenic 55.0%
CCAAGCCCCAAAGGATGACT+TGG - chr4.3:48940994-48941013 None:intergenic 55.0%
! GATGATGGCGTCCGCAATTG+GGG + chr4.3:48940640-48940659 MS.gene66650:CDS 55.0%
! TGTGCTTGCTGCCCAGAATG+CGG + chr4.3:48940876-48940895 MS.gene66650:CDS 55.0%
!! CAAGCAGGTGCTGCTTCTGT+TGG + chr4.3:48940760-48940779 MS.gene66650:CDS 55.0%
!! CCAAGTCATCCTTTGGGGCT+TGG + chr4.3:48940991-48941010 MS.gene66650:CDS 55.0%
!! CTGATGATGGCGTCCGCAAT+TGG + chr4.3:48940638-48940657 MS.gene66650:CDS 55.0%
!! CTGCTGGCTTCTTCTTCCAC+AGG + chr4.3:48940946-48940965 MS.gene66650:CDS 55.0%
GAACCGCTGCCACAAACCGT+TGG + chr4.3:48940695-48940714 MS.gene66650:CDS 60.0%
GCAGGTAAGCCAAGCCCCAA+AGG - chr4.3:48941003-48941022 None:intergenic 60.0%
GGCCGAACTGCAGCTACTGA+AGG + chr4.3:48940781-48940800 MS.gene66650:CDS 60.0%
TGGCGAGTCCGTGCCACAAA+CGG + chr4.3:48940505-48940524 MS.gene66650:CDS 60.0%


Chromosome Type Strat End Strand Name
chr4.3 gene 48940277 48941062 48940277 ID=MS.gene66650
chr4.3 mRNA 48940277 48941062 48940277 ID=MS.gene66650.t1;Parent=MS.gene66650
chr4.3 exon 48940277 48941062 48940277 ID=MS.gene66650.t1.exon1;Parent=MS.gene66650.t1
chr4.3 CDS 48940277 48941062 48940277 ID=cds.MS.gene66650.t1;Parent=MS.gene66650.t1
Gene Sequence

>MS.gene66650

ATGGATCTTATCGCTCCTGGTGTCGTCCCTAGCTTTGTCCCACTGAACCATATTGATTTTTGGGTTCAAGTTCACGGTCTGCCTTTCGGATTCATCCAACCAAAAGTAGGTCAAGGCATAGGAAGCTTCTTAGGCACTTTCAAAGCATATGATAATCGCAACTCAATTCATAGTTCCAATATGTGTGTCAGAGTCGCTATTGTTGTCACTGTCCCACTGAAAAAAGAGTGGCGAGTCCGTGCCACAAACGGTGCTTTTGTTACTGTTAACTTTAAATATGAAAAATTGGGTGTGTTTTGTCACAGGTGTGGATTGATTGGTCATACTGACAAAGTGTGTCCCGAGTTGTTCGAATTAGATTCTGATGATGGCGTCCGCAATTGGGGAGCTGACATGAAACCAGTTACGCAAAGGTTTGGAACCGCTGCCACAAACCGTTGGTTGCAAGATCCTATTCCTGCTTCTATGTCACATCAAACTCACCAAGCAGGTGCTGCTTCTGTTGGCCGAACTGCAGCTACTGAAGGTGTGGAAAATTCTGCAACTTTCAATGATCGTATGCTCACTCTTCAGATGCAACTTACTGCTATGCAACAAGATGTGCTTGCTGCCCAGAATGCGGTGCTAGCTAAAGAAGGTAAAGGTGCAAGTTCTGCTTCCAAAATACATCTGCTGGCTTCTTCTTCCACAGGTACTTCAGCTACTAGCCTGCTTCCAAGTCATCCTTTGGGGCTTGGCTTACCTGCTGCTCCACTTTCAAGTGAATCTAATGATGACATACAATAG

Protein sequence

>MS.gene66650.t1

MDLIAPGVVPSFVPLNHIDFWVQVHGLPFGFIQPKVGQGIGSFLGTFKAYDNRNSIHSSNMCVRVAIVVTVPLKKEWRVRATNGAFVTVNFKYEKLGVFCHRCGLIGHTDKVCPELFELDSDDGVRNWGADMKPVTQRFGTAATNRWLQDPIPASMSHQTHQAGAASVGRTAATEGVENSATFNDRMLTLQMQLTAMQQDVLAAQNAVLAKEGKGASSASKIHLLASSSTGTSATSLLPSHPLGLGLPAAPLSSESNDDIQ