Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66650.t1 | XP_024630605.1 | 47.5 | 255 | 126 | 2 | 1 | 255 | 67 | 313 | 2.60E-60 | 242.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66650.t1 | A0A2K3L2W5 | 60.1 | 148 | 58 | 1 | 4 | 151 | 225 | 371 | 5.2e-47 | 197.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene66650.t1 | MTR_3g053920 | 48.971 | 243 | 58 | 3 | 1 | 243 | 103 | 279 | 9.23e-62 | 196 |
| MS.gene66650.t1 | MTR_4g103530 | 86.957 | 46 | 6 | 0 | 1 | 46 | 49 | 94 | 1.49e-22 | 92.0 |
| MS.gene66650.t1 | MTR_6g033215 | 36.458 | 96 | 59 | 1 | 4 | 97 | 199 | 294 | 2.56e-12 | 65.9 |
| MS.gene66650.t1 | MTR_0010s0370 | 44.928 | 69 | 34 | 2 | 10 | 75 | 110 | 177 | 9.01e-11 | 60.1 |
| MS.gene66650.t1 | MTR_0010s0350 | 44.928 | 69 | 34 | 2 | 10 | 75 | 110 | 177 | 9.01e-11 | 60.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 58 sgRNAs with CRISPR-Local
Find 69 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTTAGCTAGCACCGCATTC+TGG | 0.141973 | 4.3:-48940888 | None:intergenic |
| AGAAGCCAGCAGATGTATTT+TGG | 0.145079 | 4.3:-48940935 | None:intergenic |
| CCAAGTCATCCTTTGGGGCT+TGG | 0.235303 | 4.3:+48940991 | MS.gene66650:CDS |
| GTTCCAAACCTTTGCGTAAC+TGG | 0.267932 | 4.3:-48940676 | None:intergenic |
| CCTATGCCTTGACCTACTTT+TGG | 0.278124 | 4.3:-48940376 | None:intergenic |
| CCTGCTTCCAAGTCATCCTT+TGG | 0.318496 | 4.3:+48940984 | MS.gene66650:CDS |
| CTGATGATGGCGTCCGCAAT+TGG | 0.330887 | 4.3:+48940638 | MS.gene66650:CDS |
| CTGCTTCCAAGTCATCCTTT+GGG | 0.362390 | 4.3:+48940985 | MS.gene66650:CDS |
| CAAGGCATAGGAAGCTTCTT+AGG | 0.376769 | 4.3:+48940388 | MS.gene66650:CDS |
| CAACCAACGGTTTGTGGCAG+CGG | 0.377733 | 4.3:-48940698 | None:intergenic |
| CAAGTTCACGGTCTGCCTTT+CGG | 0.389727 | 4.3:+48940343 | MS.gene66650:CDS |
| TGATGATGGCGTCCGCAATT+GGG | 0.395960 | 4.3:+48940639 | MS.gene66650:CDS |
| TTTAGCTAGCACCGCATTCT+GGG | 0.402038 | 4.3:-48940887 | None:intergenic |
| AGCGACTCTGACACACATAT+TGG | 0.405483 | 4.3:-48940452 | None:intergenic |
| AAACCAGTTACGCAAAGGTT+TGG | 0.415112 | 4.3:+48940673 | MS.gene66650:CDS |
| TTCGAATTAGATTCTGATGA+TGG | 0.422237 | 4.3:+48940625 | MS.gene66650:CDS |
| TGTCACAGGTGTGGATTGAT+TGG | 0.423731 | 4.3:+48940574 | MS.gene66650:CDS |
| TGCTTCCAAAATACATCTGC+TGG | 0.424718 | 4.3:+48940930 | MS.gene66650:CDS |
| TGGTTCAGTGGGACAAAGCT+AGG | 0.443161 | 4.3:-48940304 | None:intergenic |
| ATCTTGCAACCAACGGTTTG+TGG | 0.450416 | 4.3:-48940704 | None:intergenic |
| CCAAGCCCCAAAGGATGACT+TGG | 0.469128 | 4.3:-48940991 | None:intergenic |
| ACCATGGATCTTATCGCTCC+TGG | 0.475299 | 4.3:+48940274 | None:intergenic |
| CAAGCAGGTGCTGCTTCTGT+TGG | 0.476934 | 4.3:+48940760 | MS.gene66650:CDS |
| ATCTAATGATGACATACAAT+AGG | 0.483060 | 4.3:+48941041 | MS.gene66650:CDS |
| TGTGACATAGAAGCAGGAAT+AGG | 0.487822 | 4.3:-48940727 | None:intergenic |
| CCAAAGGATGACTTGGAAGC+AGG | 0.490656 | 4.3:-48940984 | None:intergenic |
| CAACAGAAGCAGCACCTGCT+TGG | 0.492986 | 4.3:-48940759 | None:intergenic |
| TCATTAGATTCACTTGAAAG+TGG | 0.496760 | 4.3:-48941027 | None:intergenic |
| AGTAACAAAAGCACCGTTTG+TGG | 0.497986 | 4.3:-48940518 | None:intergenic |
| CTGCTGGCTTCTTCTTCCAC+AGG | 0.507471 | 4.3:+48940946 | MS.gene66650:CDS |
| GATGATGGCGTCCGCAATTG+GGG | 0.507633 | 4.3:+48940640 | MS.gene66650:CDS |
| TGGCGAGTCCGTGCCACAAA+CGG | 0.511841 | 4.3:+48940505 | MS.gene66650:CDS |
| CACCTTCAGTAGCTGCAGTT+CGG | 0.513939 | 4.3:-48940783 | None:intergenic |
| TGCTTCCAAGTCATCCTTTG+GGG | 0.514970 | 4.3:+48940986 | MS.gene66650:CDS |
| TCACTTGAAAGTGGAGCAGC+AGG | 0.515569 | 4.3:-48941018 | None:intergenic |
| GGATTCATCCAACCAAAAGT+AGG | 0.533518 | 4.3:+48940364 | MS.gene66650:CDS |
| GCAGGTAAGCCAAGCCCCAA+AGG | 0.549880 | 4.3:-48941000 | None:intergenic |
| GTTTGATGTGACATAGAAGC+AGG | 0.552176 | 4.3:-48940733 | None:intergenic |
| TCATGTCAGCTCCCCAATTG+CGG | 0.560990 | 4.3:-48940651 | None:intergenic |
| GAACCGCTGCCACAAACCGT+TGG | 0.569652 | 4.3:+48940695 | MS.gene66650:CDS |
| AACTGCAGCTACTGAAGGTG+TGG | 0.577023 | 4.3:+48940786 | MS.gene66650:CDS |
| AGAATCTAATTCGAACAACT+CGG | 0.580030 | 4.3:-48940617 | None:intergenic |
| CAAAAGCACCGTTTGTGGCA+CGG | 0.590707 | 4.3:-48940513 | None:intergenic |
| GGTTCAGTGGGACAAAGCTA+GGG | 0.592996 | 4.3:-48940303 | None:intergenic |
| TCACATCAAACTCACCAAGC+AGG | 0.593385 | 4.3:+48940745 | MS.gene66650:CDS |
| GTGCTAGCTAAAGAAGGTAA+AGG | 0.593767 | 4.3:+48940898 | MS.gene66650:CDS |
| GAATCTAATTCGAACAACTC+GGG | 0.601829 | 4.3:-48940616 | None:intergenic |
| ACAAAGCTAGGGACGACACC+AGG | 0.602101 | 4.3:-48940292 | None:intergenic |
| AATGCGGTGCTAGCTAAAGA+AGG | 0.603825 | 4.3:+48940892 | MS.gene66650:CDS |
| GGCCGAACTGCAGCTACTGA+AGG | 0.610869 | 4.3:+48940781 | MS.gene66650:CDS |
| ATCCAACCAAAAGTAGGTCA+AGG | 0.617189 | 4.3:+48940370 | MS.gene66650:CDS |
| CCAAAAGTAGGTCAAGGCAT+AGG | 0.620618 | 4.3:+48940376 | MS.gene66650:CDS |
| ACCAGGAGCGATAAGATCCA+TGG | 0.622459 | 4.3:-48940275 | None:intergenic |
| GCTAGTAGCTGAAGTACCTG+TGG | 0.624706 | 4.3:-48940962 | None:intergenic |
| TGTGCTTGCTGCCCAGAATG+CGG | 0.637409 | 4.3:+48940876 | MS.gene66650:CDS |
| ACATGAAACCAGTTACGCAA+AGG | 0.638258 | 4.3:+48940668 | MS.gene66650:CDS |
| GAATAGGATCTTGCAACCAA+CGG | 0.654985 | 4.3:-48940711 | None:intergenic |
| TTTGGTTGGATGAATCCGAA+AGG | 0.672828 | 4.3:-48940358 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTTTAAATATGAAAAATT+GGG | + | chr4.3:48940544-48940563 | MS.gene66650:CDS | 10.0% |
| !! | TAACTTTAAATATGAAAAAT+TGG | + | chr4.3:48940543-48940562 | MS.gene66650:CDS | 10.0% |
| ! | AAAAATCAATATGGTTCAGT+GGG | - | chr4.3:48940318-48940337 | None:intergenic | 25.0% |
| !!! | ACTGAACCATATTGATTTTT+GGG | + | chr4.3:48940318-48940337 | MS.gene66650:CDS | 25.0% |
| AGAATCTAATTCGAACAACT+CGG | - | chr4.3:48940620-48940639 | None:intergenic | 30.0% | |
| CAAAAATCAATATGGTTCAG+TGG | - | chr4.3:48940319-48940338 | None:intergenic | 30.0% | |
| CTTGAACCCAAAAATCAATA+TGG | - | chr4.3:48940327-48940346 | None:intergenic | 30.0% | |
| TCATTAGATTCACTTGAAAG+TGG | - | chr4.3:48941030-48941049 | None:intergenic | 30.0% | |
| TTCGAATTAGATTCTGATGA+TGG | + | chr4.3:48940625-48940644 | MS.gene66650:CDS | 30.0% | |
| !!! | CACTGAACCATATTGATTTT+TGG | + | chr4.3:48940317-48940336 | MS.gene66650:CDS | 30.0% |
| GAATCTAATTCGAACAACTC+GGG | - | chr4.3:48940619-48940638 | None:intergenic | 35.0% | |
| !!! | GATTTTTGGGTTCAAGTTCA+CGG | + | chr4.3:48940331-48940350 | MS.gene66650:CDS | 35.0% |
| AAACCAGTTACGCAAAGGTT+TGG | + | chr4.3:48940673-48940692 | MS.gene66650:CDS | 40.0% | |
| ACATGAAACCAGTTACGCAA+AGG | + | chr4.3:48940668-48940687 | MS.gene66650:CDS | 40.0% | |
| ATCCAACCAAAAGTAGGTCA+AGG | + | chr4.3:48940370-48940389 | MS.gene66650:CDS | 40.0% | |
| GAATAGGATCTTGCAACCAA+CGG | - | chr4.3:48940714-48940733 | None:intergenic | 40.0% | |
| GGATTCATCCAACCAAAAGT+AGG | + | chr4.3:48940364-48940383 | MS.gene66650:CDS | 40.0% | |
| GTGCTAGCTAAAGAAGGTAA+AGG | + | chr4.3:48940898-48940917 | MS.gene66650:CDS | 40.0% | |
| GTTTGATGTGACATAGAAGC+AGG | - | chr4.3:48940736-48940755 | None:intergenic | 40.0% | |
| TGCTTCCAAAATACATCTGC+TGG | + | chr4.3:48940930-48940949 | MS.gene66650:CDS | 40.0% | |
| TGTGACATAGAAGCAGGAAT+AGG | - | chr4.3:48940730-48940749 | None:intergenic | 40.0% | |
| TTTGGTTGGATGAATCCGAA+AGG | - | chr4.3:48940361-48940380 | None:intergenic | 40.0% | |
| ! | AATTGGGTGTGTTTTGTCAC+AGG | + | chr4.3:48940560-48940579 | MS.gene66650:CDS | 40.0% |
| ! | AGAAGCCAGCAGATGTATTT+TGG | - | chr4.3:48940938-48940957 | None:intergenic | 40.0% |
| !! | AGTAACAAAAGCACCGTTTG+TGG | - | chr4.3:48940521-48940540 | None:intergenic | 40.0% |
| AGCGACTCTGACACACATAT+TGG | - | chr4.3:48940455-48940474 | None:intergenic | 45.0% | |
| ATCTTGCAACCAACGGTTTG+TGG | - | chr4.3:48940707-48940726 | None:intergenic | 45.0% | |
| CAAGGCATAGGAAGCTTCTT+AGG | + | chr4.3:48940388-48940407 | MS.gene66650:CDS | 45.0% | |
| CCAAAAGTAGGTCAAGGCAT+AGG | + | chr4.3:48940376-48940395 | MS.gene66650:CDS | 45.0% | |
| CTGCTTCCAAGTCATCCTTT+GGG | + | chr4.3:48940985-48941004 | MS.gene66650:CDS | 45.0% | |
| CTGTCCCACTGAAAAAAGAG+TGG | + | chr4.3:48940485-48940504 | MS.gene66650:CDS | 45.0% | |
| GTTCCAAACCTTTGCGTAAC+TGG | - | chr4.3:48940679-48940698 | None:intergenic | 45.0% | |
| TCACATCAAACTCACCAAGC+AGG | + | chr4.3:48940745-48940764 | MS.gene66650:CDS | 45.0% | |
| TGCTTCCAAGTCATCCTTTG+GGG | + | chr4.3:48940986-48941005 | MS.gene66650:CDS | 45.0% | |
| TTTAGCTAGCACCGCATTCT+GGG | - | chr4.3:48940890-48940909 | None:intergenic | 45.0% | |
| ! | AATGCGGTGCTAGCTAAAGA+AGG | + | chr4.3:48940892-48940911 | MS.gene66650:CDS | 45.0% |
| ! | CCTATGCCTTGACCTACTTT+TGG | - | chr4.3:48940379-48940398 | None:intergenic | 45.0% |
| ! | TGTCACAGGTGTGGATTGAT+TGG | + | chr4.3:48940574-48940593 | MS.gene66650:CDS | 45.0% |
| !! | TGCCTTGACCTACTTTTGGT+TGG | - | chr4.3:48940375-48940394 | None:intergenic | 45.0% |
| !!! | ACTCGCCACTCTTTTTTCAG+TGG | - | chr4.3:48940493-48940512 | None:intergenic | 45.0% |
| !!! | CTCGCCACTCTTTTTTCAGT+GGG | - | chr4.3:48940492-48940511 | None:intergenic | 45.0% |
| AACTGCAGCTACTGAAGGTG+TGG | + | chr4.3:48940786-48940805 | MS.gene66650:CDS | 50.0% | |
| CAAGTTCACGGTCTGCCTTT+CGG | + | chr4.3:48940343-48940362 | MS.gene66650:CDS | 50.0% | |
| CACCTTCAGTAGCTGCAGTT+CGG | - | chr4.3:48940786-48940805 | None:intergenic | 50.0% | |
| CCTGCTTCCAAGTCATCCTT+TGG | + | chr4.3:48940984-48941003 | MS.gene66650:CDS | 50.0% | |
| CTTTAGCTAGCACCGCATTC+TGG | - | chr4.3:48940891-48940910 | None:intergenic | 50.0% | |
| GGTTCAGTGGGACAAAGCTA+GGG | - | chr4.3:48940306-48940325 | None:intergenic | 50.0% | |
| TCACTTGAAAGTGGAGCAGC+AGG | - | chr4.3:48941021-48941040 | None:intergenic | 50.0% | |
| TCATGTCAGCTCCCCAATTG+CGG | - | chr4.3:48940654-48940673 | None:intergenic | 50.0% | |
| TGGTTCAGTGGGACAAAGCT+AGG | - | chr4.3:48940307-48940326 | None:intergenic | 50.0% | |
| ! | CCAAAGGATGACTTGGAAGC+AGG | - | chr4.3:48940987-48941006 | None:intergenic | 50.0% |
| ! | GCTAGTAGCTGAAGTACCTG+TGG | - | chr4.3:48940965-48940984 | None:intergenic | 50.0% |
| ! | GGTGTGTTTTGTCACAGGTG+TGG | + | chr4.3:48940565-48940584 | MS.gene66650:CDS | 50.0% |
| !! | CAAAAGCACCGTTTGTGGCA+CGG | - | chr4.3:48940516-48940535 | None:intergenic | 50.0% |
| !! | TGATGATGGCGTCCGCAATT+GGG | + | chr4.3:48940639-48940658 | MS.gene66650:CDS | 50.0% |
| ACAAAGCTAGGGACGACACC+AGG | - | chr4.3:48940295-48940314 | None:intergenic | 55.0% | |
| CAACAGAAGCAGCACCTGCT+TGG | - | chr4.3:48940762-48940781 | None:intergenic | 55.0% | |
| CAACCAACGGTTTGTGGCAG+CGG | - | chr4.3:48940701-48940720 | None:intergenic | 55.0% | |
| CCAAGCCCCAAAGGATGACT+TGG | - | chr4.3:48940994-48941013 | None:intergenic | 55.0% | |
| ! | GATGATGGCGTCCGCAATTG+GGG | + | chr4.3:48940640-48940659 | MS.gene66650:CDS | 55.0% |
| ! | TGTGCTTGCTGCCCAGAATG+CGG | + | chr4.3:48940876-48940895 | MS.gene66650:CDS | 55.0% |
| !! | CAAGCAGGTGCTGCTTCTGT+TGG | + | chr4.3:48940760-48940779 | MS.gene66650:CDS | 55.0% |
| !! | CCAAGTCATCCTTTGGGGCT+TGG | + | chr4.3:48940991-48941010 | MS.gene66650:CDS | 55.0% |
| !! | CTGATGATGGCGTCCGCAAT+TGG | + | chr4.3:48940638-48940657 | MS.gene66650:CDS | 55.0% |
| !! | CTGCTGGCTTCTTCTTCCAC+AGG | + | chr4.3:48940946-48940965 | MS.gene66650:CDS | 55.0% |
| GAACCGCTGCCACAAACCGT+TGG | + | chr4.3:48940695-48940714 | MS.gene66650:CDS | 60.0% | |
| GCAGGTAAGCCAAGCCCCAA+AGG | - | chr4.3:48941003-48941022 | None:intergenic | 60.0% | |
| GGCCGAACTGCAGCTACTGA+AGG | + | chr4.3:48940781-48940800 | MS.gene66650:CDS | 60.0% | |
| TGGCGAGTCCGTGCCACAAA+CGG | + | chr4.3:48940505-48940524 | MS.gene66650:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 48940277 | 48941062 | 48940277 | ID=MS.gene66650 |
| chr4.3 | mRNA | 48940277 | 48941062 | 48940277 | ID=MS.gene66650.t1;Parent=MS.gene66650 |
| chr4.3 | exon | 48940277 | 48941062 | 48940277 | ID=MS.gene66650.t1.exon1;Parent=MS.gene66650.t1 |
| chr4.3 | CDS | 48940277 | 48941062 | 48940277 | ID=cds.MS.gene66650.t1;Parent=MS.gene66650.t1 |
| Gene Sequence |
| Protein sequence |