AlfalfaGEDB | MS.gene28718

M. sativa cultivar XinJiangDaYe / MS.gene28718

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28718.t1	XP_024630605.1	61.8	272	66	3	37	279	48	310	9.20E-88	334

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28718.t1	A0A2K3L2W5	36.8	375	175	6	12	357	178	519	2.5e-55	225.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene03936	MS.gene28718	PPI
MS.gene05319	MS.gene28718	PPI
MS.gene038098	MS.gene28718	PPI
MS.gene28718	MS.gene85538	PPI
MS.gene28718	MS.gene025675	PPI
MS.gene56991	MS.gene28718	PPI
MS.gene28718	MS.gene26844	PPI
MS.gene60059	MS.gene28718	PPI
MS.gene28718	MS.gene055546	PPI
MS.gene28718	MS.gene71637	PPI
MS.gene28718	MS.gene55209	PPI
MS.gene28718	MS.gene074257	PPI
MS.gene28545	MS.gene28718	PPI
MS.gene28718	MS.gene073573	PPI
MS.gene038757	MS.gene28718	PPI
MS.gene064966	MS.gene28718	PPI
MS.gene28718	MS.gene068629	PPI
MS.gene28718	MS.gene009781	PPI
MS.gene43924	MS.gene28718	PPI
MS.gene046089	MS.gene28718	PPI
MS.gene047954	MS.gene28718	PPI
MS.gene28718	MS.gene21066	PPI
MS.gene036423	MS.gene28718	PPI
MS.gene28718	MS.gene009213	PPI
MS.gene28718	MS.gene013890	PPI
MS.gene28718	MS.gene98127	PPI
MS.gene28718	MS.gene003264	PPI
MS.gene28718	MS.gene054652	PPI
MS.gene72520	MS.gene28718	PPI
MS.gene28718	MS.gene45083	PPI
MS.gene66651	MS.gene28718	PPI
MS.gene28718	MS.gene049561	PPI
MS.gene28718	MS.gene008658	PPI
MS.gene35202	MS.gene28718	PPI
MS.gene28718	MS.gene80362	PPI
MS.gene28718	MS.gene49057	PPI
MS.gene051449	MS.gene28718	PPI
MS.gene28718	MS.gene89815	PPI
MS.gene63743	MS.gene28718	PPI
MS.gene28718	MS.gene048088	PPI
MS.gene28718	MS.gene86868	PPI
MS.gene48477	MS.gene28718	PPI
MS.gene28718	MS.gene058889	PPI
MS.gene28718	MS.gene034643	PPI
MS.gene30136	MS.gene28718	PPI
MS.gene008861	MS.gene28718	PPI
MS.gene024405	MS.gene28718	PPI
MS.gene012748	MS.gene28718	PPI
MS.gene83979	MS.gene28718	PPI
MS.gene28718	MS.gene012889	PPI
MS.gene28718	MS.gene34463	PPI
MS.gene85295	MS.gene28718	PPI
MS.gene28718	MS.gene038757	PPI
MS.gene28718	MS.gene000506	PPI
MS.gene47792	MS.gene28718	PPI
MS.gene29406	MS.gene28718	PPI
MS.gene46948	MS.gene28718	PPI
MS.gene98471	MS.gene28718	PPI
MS.gene28718	MS.gene51804	PPI
MS.gene28718	MS.gene013644	PPI
MS.gene93396	MS.gene28718	PPI
MS.gene05576	MS.gene28718	PPI
MS.gene05231	MS.gene28718	PPI
MS.gene010656	MS.gene28718	PPI
MS.gene28718	MS.gene010890	PPI
MS.gene28718	MS.gene061579	PPI
MS.gene041264	MS.gene28718	PPI
MS.gene41979	MS.gene28718	PPI
MS.gene64130	MS.gene28718	PPI
MS.gene050679	MS.gene28718	PPI
MS.gene28718	MS.gene04970	PPI
MS.gene20246	MS.gene28718	PPI
MS.gene055223	MS.gene28718	PPI
MS.gene28718	MS.gene060788	PPI
MS.gene28718	MS.gene43924	PPI
MS.gene071784	MS.gene28718	PPI
MS.gene28718	MS.gene41643	PPI
MS.gene053747	MS.gene28718	PPI
MS.gene28718	MS.gene027994	PPI
MS.gene76420	MS.gene28718	PPI
MS.gene65471	MS.gene28718	PPI
MS.gene28718	MS.gene022929	PPI
MS.gene061850	MS.gene28718	PPI
MS.gene28718	MS.gene26433	PPI
MS.gene28718	MS.gene007022	PPI
MS.gene26433	MS.gene28718	PPI
MS.gene28718	MS.gene69584	PPI
MS.gene28718	MS.gene52349	PPI
MS.gene28718	MS.gene021647	PPI
MS.gene042559	MS.gene28718	PPI
MS.gene28718	MS.gene048342	PPI
MS.gene28718	MS.gene069624	PPI
MS.gene98616	MS.gene28718	PPI
MS.gene042027	MS.gene28718	PPI
MS.gene85308	MS.gene28718	PPI
MS.gene28718	MS.gene073582	PPI
MS.gene69490	MS.gene28718	PPI
MS.gene015248	MS.gene28718	PPI
MS.gene28718	MS.gene016719	PPI
MS.gene28718	MS.gene028714	PPI
MS.gene28718	MS.gene88393	PPI
MS.gene28718	MS.gene048706	PPI
MS.gene80362	MS.gene28718	PPI
MS.gene28718	MS.gene69586	PPI
MS.gene44083	MS.gene28718	PPI
MS.gene28718	MS.gene053373	PPI
MS.gene013193	MS.gene28718	PPI
MS.gene04970	MS.gene28718	PPI
MS.gene08106	MS.gene28718	PPI
MS.gene58419	MS.gene28718	PPI
MS.gene28718	MS.gene63150	PPI
MS.gene28718	MS.gene03936	PPI
MS.gene067340	MS.gene28718	PPI
MS.gene28718	MS.gene072852	PPI
MS.gene28718	MS.gene65474	PPI
MS.gene23521	MS.gene28718	PPI
MS.gene28718	MS.gene08708	PPI
MS.gene28718	MS.gene06164	PPI
MS.gene28718	MS.gene50392	PPI
MS.gene28718	MS.gene86275	PPI
MS.gene28718	MS.gene22730	PPI
MS.gene28718	MS.gene032299	PPI
MS.gene28718	MS.gene017586	PPI
MS.gene87529	MS.gene28718	PPI
MS.gene04134	MS.gene28718	PPI
MS.gene28718	MS.gene037634	PPI
MS.gene28718	MS.gene057828	PPI
MS.gene062067	MS.gene28718	PPI
MS.gene28718	MS.gene08606	PPI
MS.gene28718	MS.gene000640	PPI
MS.gene57044	MS.gene28718	PPI
MS.gene28718	MS.gene98756	PPI
MS.gene038676	MS.gene28718	PPI
MS.gene28718	MS.gene028716	PPI
MS.gene28718	MS.gene74639	PPI
MS.gene28718	MS.gene071133	PPI
MS.gene28718	MS.gene60266	PPI
MS.gene28718	MS.gene98467	PPI
MS.gene71637	MS.gene28718	PPI
MS.gene28718	MS.gene62249	PPI
MS.gene042651	MS.gene28718	PPI
MS.gene28718	MS.gene024522	PPI
MS.gene28718	MS.gene54051	PPI
MS.gene28718	MS.gene22111	PPI
MS.gene28718	MS.gene049610	PPI
MS.gene28718	MS.gene010290	PPI
MS.gene28718	MS.gene37955	PPI
MS.gene28718	MS.gene92517	PPI
MS.gene28718	MS.gene36069	PPI
MS.gene28718	MS.gene045875	PPI
MS.gene28718	MS.gene84790	PPI
MS.gene28718	MS.gene044729	PPI
MS.gene034643	MS.gene28718	PPI
MS.gene28718	MS.gene60390	PPI
MS.gene28718	MS.gene039692	PPI
MS.gene28718	MS.gene55682	PPI
MS.gene71588	MS.gene28718	PPI
MS.gene28718	MS.gene002590	PPI
MS.gene04749	MS.gene28718	PPI
MS.gene28718	MS.gene77990	PPI
MS.gene28718	MS.gene062511	PPI
MS.gene061579	MS.gene28718	PPI
MS.gene071320	MS.gene28718	PPI
MS.gene28718	MS.gene059269	PPI
MS.gene42903	MS.gene28718	PPI
MS.gene81712	MS.gene28718	PPI
MS.gene28718	MS.gene057934	PPI
MS.gene28718	MS.gene024841	PPI
MS.gene026680	MS.gene28718	PPI
MS.gene021555	MS.gene28718	PPI
MS.gene048747	MS.gene28718	PPI
MS.gene28718	MS.gene76420	PPI
MS.gene28718	MS.gene46353	PPI
MS.gene28718	MS.gene40828	PPI
MS.gene22200	MS.gene28718	PPI
MS.gene28718	MS.gene51799	PPI
MS.gene28718	MS.gene98471	PPI
MS.gene28718	MS.gene61651	PPI
MS.gene26038	MS.gene28718	PPI
MS.gene28718	MS.gene29406	PPI
MS.gene031967	MS.gene28718	PPI
MS.gene28718	MS.gene09023	PPI
MS.gene34812	MS.gene28718	PPI
MS.gene28718	MS.gene054508	PPI
MS.gene28718	MS.gene025987	PPI
MS.gene02896	MS.gene28718	PPI
MS.gene28718	MS.gene95960	PPI
MS.gene063214	MS.gene28718	PPI
MS.gene28718	MS.gene043140	PPI
MS.gene97410	MS.gene28718	PPI
MS.gene32415	MS.gene28718	PPI
MS.gene28718	MS.gene041264	PPI
MS.gene28718	MS.gene03149	PPI
MS.gene035626	MS.gene28718	PPI
MS.gene28718	MS.gene29321	PPI
MS.gene65623	MS.gene28718	PPI
MS.gene71675	MS.gene28718	PPI
MS.gene28718	MS.gene025401	PPI
MS.gene69495	MS.gene28718	PPI
MS.gene049512	MS.gene28718	PPI
MS.gene28718	MS.gene015093	PPI
MS.gene038623	MS.gene28718	PPI
MS.gene28718	MS.gene24278	PPI
MS.gene05357	MS.gene28718	PPI
MS.gene28718	MS.gene000379	PPI
MS.gene28718	MS.gene34618	PPI
MS.gene032311	MS.gene28718	PPI
MS.gene28718	MS.gene59460	PPI
MS.gene030090	MS.gene28718	PPI
MS.gene96174	MS.gene28718	PPI
MS.gene28718	MS.gene66650	PPI
MS.gene018196	MS.gene28718	PPI
MS.gene86849	MS.gene28718	PPI
MS.gene28718	MS.gene08713	PPI
MS.gene57019	MS.gene28718	PPI
MS.gene28718	MS.gene09517	PPI
MS.gene28718	MS.gene87845	PPI
MS.gene39956	MS.gene28718	PPI
MS.gene021968	MS.gene28718	PPI
MS.gene28718	MS.gene58419	PPI
MS.gene09589	MS.gene28718	PPI
MS.gene28718	MS.gene64178	PPI
MS.gene43393	MS.gene28718	PPI
MS.gene34197	MS.gene28718	PPI
MS.gene28718	MS.gene44144	PPI
MS.gene008275	MS.gene28718	PPI
MS.gene28718	MS.gene05576	PPI
MS.gene28718	MS.gene060718	PPI
MS.gene005120	MS.gene28718	PPI
MS.gene28718	MS.gene48477	PPI
MS.gene048072	MS.gene28718	PPI
MS.gene28718	MS.gene027169	PPI
MS.gene060386	MS.gene28718	PPI
MS.gene28718	MS.gene004417	PPI
MS.gene28718	MS.gene019177	PPI
MS.gene05436	MS.gene28718	PPI
MS.gene059736	MS.gene28718	PPI
MS.gene70932	MS.gene28718	PPI
MS.gene35175	MS.gene28718	PPI
MS.gene28718	MS.gene055560	PPI
MS.gene28718	MS.gene024297	PPI
MS.gene28718	MS.gene37537	PPI
MS.gene021965	MS.gene28718	PPI
MS.gene055683	MS.gene28718	PPI
MS.gene042614	MS.gene28718	PPI
MS.gene28718	MS.gene069786	PPI
MS.gene28718	MS.gene42010	PPI
MS.gene28718	MS.gene025158	PPI
MS.gene96109	MS.gene28718	PPI
MS.gene28718	MS.gene27329	PPI
MS.gene28718	MS.gene41393	PPI
MS.gene28718	MS.gene016632	PPI
MS.gene28718	MS.gene018474	PPI
MS.gene009121	MS.gene28718	PPI
MS.gene94654	MS.gene28718	PPI
MS.gene28718	MS.gene40469	PPI
MS.gene28718	MS.gene96163	PPI
MS.gene36069	MS.gene28718	PPI
MS.gene61452	MS.gene28718	PPI
MS.gene83772	MS.gene28718	PPI
MS.gene008927	MS.gene28718	PPI
MS.gene016102	MS.gene28718	PPI
MS.gene46941	MS.gene28718	PPI
MS.gene045614	MS.gene28718	PPI
MS.gene96541	MS.gene28718	PPI
MS.gene000634	MS.gene28718	PPI
MS.gene63762	MS.gene28718	PPI
MS.gene30208	MS.gene28718	PPI
MS.gene025873	MS.gene28718	PPI
MS.gene28718	MS.gene037049	PPI
MS.gene012019	MS.gene28718	PPI
MS.gene28718	MS.gene026680	PPI
MS.gene28718	MS.gene61499	PPI
MS.gene013676	MS.gene28718	PPI
MS.gene05358	MS.gene28718	PPI
MS.gene28718	MS.gene019165	PPI
MS.gene95561	MS.gene28718	PPI
MS.gene28718	MS.gene81194	PPI
MS.gene28718	MS.gene060773	PPI
MS.gene065627	MS.gene28718	PPI
MS.gene28718	MS.gene038394	PPI
MS.gene018745	MS.gene28718	PPI
MS.gene000629	MS.gene28718	PPI
MS.gene65546	MS.gene28718	PPI
MS.gene28718	MS.gene019001	PPI
MS.gene28718	MS.gene53904	PPI
MS.gene28718	MS.gene24070	PPI
MS.gene28718	MS.gene73261	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene28718.t1	MTR_3g053920	44.444	261	107	4	11	271	58	280	3.38e-60	196
MS.gene28718.t1	MTR_4g103530	68.675	83	26	0	9	91	2	84	5.18e-36	130
MS.gene28718.t1	MTR_7g011710	67.073	82	27	0	10	91	3	84	1.66e-34	125
MS.gene28718.t1	MTR_2g061500	71.233	73	21	0	10	82	57	129	2.27e-33	121
MS.gene28718.t1	MTR_6g033215	27.273	143	73	1	12	123	152	294	1.45e-17	82.4
MS.gene28718.t1	MTR_0010s0370	39.080	87	50	3	7	91	51	136	3.36e-13	68.2
MS.gene28718.t1	MTR_0010s0350	39.080	87	50	3	7	91	51	136	3.36e-13	68.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 111 sgRNAs with CRISPR-Local

Find 234 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTCAAACTTGTCCAATT+TGG	0.111128	4.3:+19399054	MS.gene28718:CDS
GGACCTGCCATTGCTTCTTT+AGG	0.166134	4.3:-19398739	None:intergenic
GACCTGATATCCTCTGTTTC+AGG	0.218469	4.3:-19399322	None:intergenic
TTTGCAGCTGATGGGATTAA+TGG	0.226048	4.3:+19399421	MS.gene28718:CDS
ATCTGAACCATCTAGATCTT+TGG	0.231408	4.3:+19398565	MS.gene28718:CDS
GACCTGCCATTGCTTCTTTA+GGG	0.246581	4.3:-19398738	None:intergenic
TGCTATTAAACAAGGAATTA+TGG	0.273925	4.3:+19399111	MS.gene28718:CDS
GTCCCAATACGGTGGACATT+AGG	0.286468	4.3:-19398923	None:intergenic
ACTGAGTCGGCCACCCAAAT+TGG	0.289592	4.3:-19399068	None:intergenic
TTTGTTTGCTGTACAACATT+TGG	0.303565	4.3:-19398980	None:intergenic
GAGTCAAGTTCAAATAAATT+AGG	0.315354	4.3:-19398863	None:intergenic
TCTTCAAACTTGTCCAATTT+GGG	0.322600	4.3:+19399055	MS.gene28718:CDS
GCGGCACCTCTTTCCATTAC+AGG	0.327196	4.3:+19399271	MS.gene28718:CDS
CGCCCTAATGTCCACCGTAT+TGG	0.347990	4.3:+19398920	MS.gene28718:CDS
TTGCAGCTGATGGGATTAAT+GGG	0.358856	4.3:+19399422	MS.gene28718:CDS
TCTGCTTGTATTTCTGCTGC+TGG	0.373391	4.3:+19399211	MS.gene28718:CDS
CAATTTAAATATGAAAAGTT+GGG	0.374919	4.3:+19398791	MS.gene28718:CDS
GCCCTAATGTCCACCGTATT+GGG	0.379728	4.3:+19398921	MS.gene28718:CDS
ATTTGTGATGGAACTGAAAC+AGG	0.379761	4.3:-19398443	None:intergenic
GACGGGTTTCCACGCTTTGA+CGG	0.382350	4.3:-19398382	None:intergenic
CCAATTTAAATATGAAAAGT+TGG	0.392300	4.3:+19398790	MS.gene28718:CDS
TCCCAATACGGTGGACATTA+GGG	0.395254	4.3:-19398922	None:intergenic
TCTGAACCATCTAGATCTTT+GGG	0.396460	4.3:+19398566	MS.gene28718:CDS
TGGCAGGTCCGCTCAAACAA+TGG	0.400450	4.3:+19398752	MS.gene28718:CDS
TTGGGACTGCAGCCACTAAC+AGG	0.407010	4.3:+19398939	MS.gene28718:CDS
TGGCTTGTCCCACCCTTTGT+TGG	0.412829	4.3:-19398620	None:intergenic
CAGCAAACAAATGGCACTAA+TGG	0.417841	4.3:+19398992	MS.gene28718:CDS
TGACGGGTTCCCAATGCTTA+CGG	0.417937	4.3:-19398365	None:intergenic
TGCTGTACAACATTTGGAAT+GGG	0.425208	4.3:-19398974	None:intergenic
CTTCATGAGGCGCACCACTT+TGG	0.433502	4.3:-19399384	None:intergenic
TGATGATGGTGTACGTGAAT+GGG	0.434942	4.3:+19398886	MS.gene28718:CDS
CAAGTTCACGGACTGCCCTT+TGG	0.437581	4.3:+19398591	MS.gene28718:CDS
ACGGGTTTCCACGCTTTGAC+GGG	0.447183	4.3:-19398381	None:intergenic
GTCTTGAAGCCACCTGTTAG+TGG	0.448129	4.3:-19398951	None:intergenic
CCTAAAAGGCCTCTGGTGCT+TGG	0.454169	4.3:+19399241	MS.gene28718:CDS
AGCCTGAAACAGAGGATATC+AGG	0.456205	4.3:+19399320	MS.gene28718:CDS
GAAATATGAACCGTAAGCAT+TGG	0.464122	4.3:+19398355	None:intergenic
AAATATGAACCGTAAGCATT+GGG	0.466335	4.3:+19398356	None:intergenic
TTTGAACTTGACTCTGATGA+TGG	0.467042	4.3:+19398872	MS.gene28718:CDS
AACATGTTCAGCACCTGTAA+TGG	0.472241	4.3:-19399284	None:intergenic
CTTTGGTTTCATCCAACAAA+GGG	0.475670	4.3:+19398608	MS.gene28718:CDS
GGCAGGTCCGCTCAAACAAT+GGG	0.477547	4.3:+19398753	MS.gene28718:CDS
TCCTCTGTTTCAGGCTCGGT+AGG	0.485474	4.3:-19399313	None:intergenic
ACGTTTCGTGGAACAGCACC+TGG	0.486510	4.3:-19398540	None:intergenic
TGAAGATGAGGACGCTGCAC+CGG	0.490084	4.3:-19400263	None:intergenic
TGCCTTGTCTAAAAGTGGTA+AGG	0.493296	4.3:+19399147	MS.gene28718:CDS
CTGATGATGGTGTACGTGAA+TGG	0.493705	4.3:+19398885	MS.gene28718:CDS
GGGTTTCTATTGAAAAGAAT+AGG	0.494933	4.3:-19400242	None:intergenic
GAAGATGAGGACGCTGCACC+GGG	0.499132	4.3:-19400262	None:intergenic
GAAAATTCAGTTTCTTCATG+AGG	0.499827	4.3:-19399397	None:intergenic
GATCTTTGGGTACAAGTTCA+CGG	0.501951	4.3:+19398579	MS.gene28718:CDS
AATGTTGTACAGCAAACAAA+TGG	0.503542	4.3:+19398983	MS.gene28718:CDS
CCTCTGTTTCAGGCTCGGTA+GGG	0.506009	4.3:-19399312	None:intergenic
AATTCTGCCTTGTCTAAAAG+TGG	0.507288	4.3:+19399142	MS.gene28718:CDS
TGGACCATCATCGAGGACTT+TGG	0.507933	4.3:-19398475	None:intergenic
TATTGAAAAGAATAGGATAG+TGG	0.519811	4.3:-19400235	None:intergenic
TGTTACCGTTGCGGTCTGCT+CGG	0.520212	4.3:+19398821	MS.gene28718:CDS
TGGCAGCATCTAATTTGTGA+TGG	0.521268	4.3:-19398455	None:intergenic
TTGCTGTACAACATTTGGAA+TGG	0.522516	4.3:-19398975	None:intergenic
CTCTGTTTCAGGCTCGGTAG+GGG	0.525207	4.3:-19399311	None:intergenic
ACCTGCCATTGCTTCTTTAG+GGG	0.525977	4.3:-19398737	None:intergenic
GAAGGAAACTGAGAGGCAGC+AGG	0.533011	4.3:-19399025	None:intergenic
AAATACAAGCAGAAGTGGAC+GGG	0.533566	4.3:-19399201	None:intergenic
AGCAGCAAGGTTCTCTAAAG+TGG	0.534896	4.3:-19400201	None:intergenic
GATATCCTCTGTTTCAGGCT+CGG	0.539302	4.3:-19399317	None:intergenic
TGGTTCAGATCAACGTTTCG+TGG	0.546417	4.3:-19398552	None:intergenic
CCTTTGGTTTCATCCAACAA+AGG	0.546455	4.3:+19398607	MS.gene28718:CDS
GATGATGGTGTACGTGAATG+GGG	0.546601	4.3:+19398887	MS.gene28718:CDS
AATTTAAATATGAAAAGTTG+GGG	0.547584	4.3:+19398792	MS.gene28718:CDS
CTTGTACCCAAAGATCTAGA+TGG	0.548240	4.3:-19398572	None:intergenic
CATAATTCCCATTGTTTGAG+CGG	0.549445	4.3:-19398760	None:intergenic
AGCAGAAATACAAGCAGAAG+TGG	0.552878	4.3:-19399206	None:intergenic
GAAATACAAGCAGAAGTGGA+CGG	0.556285	4.3:-19399202	None:intergenic
GCTGCCAAAGTCCTCGATGA+TGG	0.556307	4.3:+19398471	MS.gene28718:CDS
CCCTACCGAGCCTGAAACAG+AGG	0.565438	4.3:+19399312	MS.gene28718:CDS
CTTTGTTGGATGAAACCAAA+GGG	0.565476	4.3:-19398606	None:intergenic
GCCCCTAAAGAAGCAATGGC+AGG	0.569503	4.3:+19398736	MS.gene28718:CDS
TCAAACTTGTCCAATTTGGG+TGG	0.570842	4.3:+19399058	MS.gene28718:CDS
TAGTGGCTGCAGTCCCAATA+CGG	0.577198	4.3:-19398934	None:intergenic
CAGAAGTGGACGGGAAAGCA+TGG	0.584438	4.3:-19399192	None:intergenic
AAGTTTGAAGAACTTTCAGA+AGG	0.586633	4.3:-19399043	None:intergenic
TCAGCACCTGTAATGGAAAG+AGG	0.588188	4.3:-19399277	None:intergenic
ATGATGGTGTACGTGAATGG+GGG	0.589038	4.3:+19398888	MS.gene28718:CDS
ATCAGCCCCTAAAGAAGCAA+TGG	0.593819	4.3:+19398732	MS.gene28718:CDS
AAGTCCTCGATGATGGTCCA+TGG	0.595595	4.3:+19398478	MS.gene28718:CDS
TGCAGCTGATGGGATTAATG+GGG	0.596716	4.3:+19399423	MS.gene28718:CDS
GCTGTACAACATTTGGAATG+GGG	0.599803	4.3:-19398973	None:intergenic
CAAACAAATGGCACTAATGG+AGG	0.607950	4.3:+19398995	MS.gene28718:CDS
AAACTACGTTTATGAAGATG+AGG	0.613762	4.3:-19400275	None:intergenic
GAGATAAATGCTATTAAACA+AGG	0.615670	4.3:+19399103	MS.gene28718:CDS
TGAAAATTATCATAGAGCCA+TGG	0.620015	4.3:-19398495	None:intergenic
GTCATGGAAAGAATCAGTCC+AGG	0.620923	4.3:+19398522	MS.gene28718:CDS
CATGATGAACATCACTGAGT+CGG	0.625427	4.3:-19399081	None:intergenic
TGATGGTGTACGTGAATGGG+GGG	0.627050	4.3:+19398889	MS.gene28718:CDS
ATAGGATAGTGGTCTGAGGA+CGG	0.630016	4.3:-19400224	None:intergenic
GTAATGGAAAGAGGTGCCGC+AGG	0.640977	4.3:-19399268	None:intergenic
ATTGGGAACCCGTCAAAGCG+TGG	0.646937	4.3:+19398373	MS.gene28718:CDS
AAGAATAGGATAGTGGTCTG+AGG	0.656694	4.3:-19400228	None:intergenic
GCAGGTATACCAAGCACCAG+AGG	0.661474	4.3:-19399250	None:intergenic
GGACTGCAGCCACTAACAGG+TGG	0.673886	4.3:+19398942	MS.gene28718:CDS
CCTTTGTTGGATGAAACCAA+AGG	0.680480	4.3:-19398607	None:intergenic
TCTGCTCGGTCATACTGACA+AGG	0.685929	4.3:+19398835	MS.gene28718:CDS
TCTGGTGCTTGGTATACCTG+CGG	0.686120	4.3:+19399252	MS.gene28718:CDS
TATGACCGAGCAGACCGCAA+CGG	0.689782	4.3:-19398826	None:intergenic
CTAGCTGAATGTCTCCAAAG+TGG	0.691586	4.3:+19399370	MS.gene28718:CDS
GATGGAACTGAAACAGGTAA+CGG	0.692363	4.3:-19398437	None:intergenic
ATTTAAATATGAAAAGTTGG+GGG	0.694275	4.3:+19398793	MS.gene28718:CDS
TGGCTGCAGTCCCAATACGG+TGG	0.704005	4.3:-19398931	None:intergenic
GGTCTGAGGACGGAGCAGCA+AGG	0.720866	4.3:-19400214	None:intergenic
ACTTTCAGAAGGAAACTGAG+AGG	0.721193	4.3:-19399032	None:intergenic
AGAGCCATGGACCATCATCG+AGG	0.748341	4.3:-19398482	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTAAATATGAAAAGTTG+GGG	+	chr4.3:19398792-19398811	MS.gene28718:CDS	15.0%
!!	CAATTTAAATATGAAAAGTT+GGG	+	chr4.3:19398791-19398810	MS.gene28718:CDS	15.0%
!!	TACAATATTACAATTAAAAG+AGG	-	chr4.3:19399790-19399809	None:intergenic	15.0%
!!!	GTAAATCTTCAATTTTATTA+CGG	-	chr4.3:19399689-19399708	None:intergenic	15.0%
!!!	TAAATCTTCAATTTTATTAC+GGG	-	chr4.3:19399688-19399707	None:intergenic	15.0%
!!	ATTTAAATATGAAAAGTTGG+GGG	+	chr4.3:19398793-19398812	MS.gene28718:CDS	20.0%
!!	CCAATTTAAATATGAAAAGT+TGG	+	chr4.3:19398790-19398809	MS.gene28718:CDS	20.0%
!!!	AAAATTGTAACTTTTTTGAC+GGG	-	chr4.3:19398402-19398421	None:intergenic	20.0%
!!!	CAAAATTGTAACTTTTTTGA+CGG	-	chr4.3:19398403-19398422	None:intergenic	20.0%
!!!	CCAACTTTTCATATTTAAAT+TGG	-	chr4.3:19398793-19398812	None:intergenic	20.0%
!!!	TGATAATTTTCATATTGTCA+TGG	+	chr4.3:19398506-19398525	MS.gene28718:CDS	20.0%
!	AAAGCCATTAATTAACCAAT+TGG	-	chr4.3:19400030-19400049	None:intergenic	25.0%
!	ATCAACAGAGATATGATTAT+TGG	-	chr4.3:19399820-19399839	None:intergenic	25.0%
!	ATTGATTAGATAATTGTCGA+AGG	-	chr4.3:19399929-19399948	None:intergenic	25.0%
!	ATTGGTTAATTAATGGCTTT+AGG	+	chr4.3:19400030-19400049	MS.gene28718:intron	25.0%
!	CAATTATCTAATCAATGTAC+AGG	+	chr4.3:19399933-19399952	MS.gene28718:intron	25.0%
!	GAGATAAATGCTATTAAACA+AGG	+	chr4.3:19399103-19399122	MS.gene28718:CDS	25.0%
!	GAGTCAAGTTCAAATAAATT+AGG	-	chr4.3:19398866-19398885	None:intergenic	25.0%
!	TATTGAAAAGAATAGGATAG+TGG	-	chr4.3:19400238-19400257	None:intergenic	25.0%
!	TGCTATTAAACAAGGAATTA+TGG	+	chr4.3:19399111-19399130	MS.gene28718:CDS	25.0%
!	TGGTGACTTTAATGATTTAA+TGG	+	chr4.3:19399971-19399990	MS.gene28718:intron	25.0%
!!	AAAGTTACAATTTTGCAATC+TGG	+	chr4.3:19398408-19398427	MS.gene28718:CDS	25.0%
!!	ATCTTCATAAACGTAGTTTT+CGG	+	chr4.3:19400279-19400298	MS.gene28718:CDS	25.0%
!!	TATTCTTTTCAATAGAAACC+CGG	+	chr4.3:19400244-19400263	MS.gene28718:CDS	25.0%
	AAACTACGTTTATGAAGATG+AGG	-	chr4.3:19400278-19400297	None:intergenic	30.0%
	AAGATTAAACCAGTCACTAT+TGG	-	chr4.3:19400171-19400190	None:intergenic	30.0%
	AAGTTTGAAGAACTTTCAGA+AGG	-	chr4.3:19399046-19399065	None:intergenic	30.0%
	AATGTTGTACAGCAAACAAA+TGG	+	chr4.3:19398983-19399002	MS.gene28718:CDS	30.0%
	CGTTCCAATTGGTTAATTAA+TGG	+	chr4.3:19400023-19400042	MS.gene28718:intron	30.0%
	GAAAATTCAGTTTCTTCATG+AGG	-	chr4.3:19399400-19399419	None:intergenic	30.0%
	GAAGATTTACGTTATTTGCT+TGG	+	chr4.3:19399699-19399718	MS.gene28718:intron	30.0%
	GGGTTTCTATTGAAAAGAAT+AGG	-	chr4.3:19400245-19400264	None:intergenic	30.0%
	GTATGAAAACCATGATGTAT+CGG	+	chr4.3:19399513-19399532	MS.gene28718:intron	30.0%
	TAATTAACCAATTGGAACGA+AGG	-	chr4.3:19400022-19400041	None:intergenic	30.0%
	TCAGGTCTGAATTGAAAAAA+AGG	+	chr4.3:19399338-19399357	MS.gene28718:CDS	30.0%
	TCTTCAAACTTGTCCAATTT+GGG	+	chr4.3:19399055-19399074	MS.gene28718:CDS	30.0%
	TGAAAATTATCATAGAGCCA+TGG	-	chr4.3:19398498-19398517	None:intergenic	30.0%
	TTCTTCAAACTTGTCCAATT+TGG	+	chr4.3:19399054-19399073	MS.gene28718:CDS	30.0%
	TTTAAATCCGGAATTGCATT+CGG	-	chr4.3:19399609-19399628	None:intergenic	30.0%
!	AATTTTCTTTTGCAGCTGAT+GGG	+	chr4.3:19399413-19399432	MS.gene28718:CDS	30.0%
!	ACATCATGGTTTTCATACAA+AGG	-	chr4.3:19399511-19399530	None:intergenic	30.0%
!	ATTAATGGGGATTTTTCCTT+AGG	+	chr4.3:19399436-19399455	MS.gene28718:CDS	30.0%
!	CGGATTTAAAATACCTAGTT+CGG	+	chr4.3:19399619-19399638	MS.gene28718:intron	30.0%
!!	CGAACTAGGTATTTTAAATC+CGG	-	chr4.3:19399621-19399640	None:intergenic	30.0%
!!	TTTGTTTGCTGTACAACATT+TGG	-	chr4.3:19398983-19399002	None:intergenic	30.0%
	AAACTCTTGAACCAAGTGAA+AGG	-	chr4.3:19400104-19400123	None:intergenic	35.0%
	AACTCTTGAACCAAGTGAAA+GGG	-	chr4.3:19400103-19400122	None:intergenic	35.0%
	AAGATTTAGTAGCTACTGCT+TGG	+	chr4.3:19400336-19400355	MS.gene28718:intron	35.0%
	AATTCTGCCTTGTCTAAAAG+TGG	+	chr4.3:19399142-19399161	MS.gene28718:CDS	35.0%
	ACGTCTCACTAAGAAATAAG+AGG	-	chr4.3:19399659-19399678	None:intergenic	35.0%
	ATCTGAACCATCTAGATCTT+TGG	+	chr4.3:19398565-19398584	MS.gene28718:CDS	35.0%
	ATGGATGCTAGTGAAAAAAG+AGG	+	chr4.3:19399990-19400009	MS.gene28718:intron	35.0%
	ATTGATAGGATGGAATTGCA+GGG	+	chr4.3:19399572-19399591	MS.gene28718:intron	35.0%
	CATAATTCCCATTGTTTGAG+CGG	-	chr4.3:19398763-19398782	None:intergenic	35.0%
	CCTTAGGTGAAAATGTTAGT+GGG	+	chr4.3:19399452-19399471	MS.gene28718:intron	35.0%
	CTTAGGTGAAAATGTTAGTG+GGG	+	chr4.3:19399453-19399472	MS.gene28718:intron	35.0%
	CTTTGTTGGATGAAACCAAA+GGG	-	chr4.3:19398609-19398628	None:intergenic	35.0%
	GAGAAATGAGATTGATAGGA+TGG	+	chr4.3:19399562-19399581	MS.gene28718:intron	35.0%
	GATGTTACTGATGTTGTTCA+TGG	+	chr4.3:19399837-19399856	MS.gene28718:intron	35.0%
	TCACTAAGAAATAAGAGGTC+CGG	-	chr4.3:19399654-19399673	None:intergenic	35.0%
	TCCTTAGGTGAAAATGTTAG+TGG	+	chr4.3:19399451-19399470	MS.gene28718:intron	35.0%
	TCTGAACCATCTAGATCTTT+GGG	+	chr4.3:19398566-19398585	MS.gene28718:CDS	35.0%
	TGGATGCTAGTGAAAAAAGA+GGG	+	chr4.3:19399991-19400010	MS.gene28718:intron	35.0%
	TTAAATCCGGAATTGCATTC+GGG	-	chr4.3:19399608-19399627	None:intergenic	35.0%
	TTAGGTGAAAATGTTAGTGG+GGG	+	chr4.3:19399454-19399473	MS.gene28718:intron	35.0%
	TTCACTTGGTTCAAGAGTTT+AGG	+	chr4.3:19400104-19400123	MS.gene28718:intron	35.0%
	TTGCTGTACAACATTTGGAA+TGG	-	chr4.3:19398978-19398997	None:intergenic	35.0%
	TTTGAACTTGACTCTGATGA+TGG	+	chr4.3:19398872-19398891	MS.gene28718:CDS	35.0%
!	ATTTGTGATGGAACTGAAAC+AGG	-	chr4.3:19398446-19398465	None:intergenic	35.0%
!	CTTTGGTTTCATCCAACAAA+GGG	+	chr4.3:19398608-19398627	MS.gene28718:CDS	35.0%
!	GAATTTTCTTTTGCAGCTGA+TGG	+	chr4.3:19399412-19399431	MS.gene28718:CDS	35.0%
!	GGAGAAATGAAAGTGTATGA+TGG	+	chr4.3:19398656-19398675	MS.gene28718:intron	35.0%
!	TGCTGTACAACATTTGGAAT+GGG	-	chr4.3:19398977-19398996	None:intergenic	35.0%
!	TGTTTTTCTGTTGATCGTAG+TGG	+	chr4.3:19399732-19399751	MS.gene28718:intron	35.0%
!!	AACCTTACCACTTTTAGACA+AGG	-	chr4.3:19399152-19399171	None:intergenic	35.0%
!!	AATTAATGGCTTTAGGCAAG+CGG	+	chr4.3:19400037-19400056	MS.gene28718:intron	35.0%
!!	GCTACTAAATCTTTGAAACC+TGG	-	chr4.3:19400329-19400348	None:intergenic	35.0%
	AAATACAAGCAGAAGTGGAC+GGG	-	chr4.3:19399204-19399223	None:intergenic	40.0%
	AACATGTTCAGCACCTGTAA+TGG	-	chr4.3:19399287-19399306	None:intergenic	40.0%
	ACAAGCCATGGTCAATTTCT+CGG	+	chr4.3:19398635-19398654	MS.gene28718:intron	40.0%
	ACTTTCAGAAGGAAACTGAG+AGG	-	chr4.3:19399035-19399054	None:intergenic	40.0%
	AGCAGAAATACAAGCAGAAG+TGG	-	chr4.3:19399209-19399228	None:intergenic	40.0%
	AGTGTATGATGGTCGTAACT+CGG	+	chr4.3:19398667-19398686	MS.gene28718:intron	40.0%
	CAAACAAATGGCACTAATGG+AGG	+	chr4.3:19398995-19399014	MS.gene28718:CDS	40.0%
	CAGCAAACAAATGGCACTAA+TGG	+	chr4.3:19398992-19399011	MS.gene28718:CDS	40.0%
	CAGTTCATACATGCGTTTGA+AGG	+	chr4.3:19398694-19398713	MS.gene28718:intron	40.0%
	CATGATGAACATCACTGAGT+CGG	-	chr4.3:19399084-19399103	None:intergenic	40.0%
	CCTATCAATCTCATTTCTCC+CGG	-	chr4.3:19399561-19399580	None:intergenic	40.0%
	CCTTTGTTGGATGAAACCAA+AGG	-	chr4.3:19398610-19398629	None:intergenic	40.0%
	CTTGTACCCAAAGATCTAGA+TGG	-	chr4.3:19398575-19398594	None:intergenic	40.0%
	GAAATACAAGCAGAAGTGGA+CGG	-	chr4.3:19399205-19399224	None:intergenic	40.0%
	GATCTTTGGGTACAAGTTCA+CGG	+	chr4.3:19398579-19398598	MS.gene28718:CDS	40.0%
	GATGGAACTGAAACAGGTAA+CGG	-	chr4.3:19398440-19398459	None:intergenic	40.0%
	GATTGATAGGATGGAATTGC+AGG	+	chr4.3:19399571-19399590	MS.gene28718:intron	40.0%
	GCTGCACAATGAGTTAACTT+AGG	-	chr4.3:19400389-19400408	None:intergenic	40.0%
	GTACGAAAATGCGTGGTATT+TGG	+	chr4.3:19400301-19400320	MS.gene28718:intron	40.0%
	TAGGTGAAAATGTTAGTGGG+GGG	+	chr4.3:19399455-19399474	MS.gene28718:intron	40.0%
	TCAAACTTGTCCAATTTGGG+TGG	+	chr4.3:19399058-19399077	MS.gene28718:CDS	40.0%
	TGCCTTGTCTAAAAGTGGTA+AGG	+	chr4.3:19399147-19399166	MS.gene28718:CDS	40.0%
	TTGATAGGATGGAATTGCAG+GGG	+	chr4.3:19399573-19399592	MS.gene28718:intron	40.0%
	TTGCAGCTGATGGGATTAAT+GGG	+	chr4.3:19399422-19399441	MS.gene28718:CDS	40.0%
	TTTCTCCGAGAAATTGACCA+TGG	-	chr4.3:19398643-19398662	None:intergenic	40.0%
	TTTGCAGCTGATGGGATTAA+TGG	+	chr4.3:19399421-19399440	MS.gene28718:CDS	40.0%
!	AAGAATAGGATAGTGGTCTG+AGG	-	chr4.3:19400231-19400250	None:intergenic	40.0%
!	CCCACTAACATTTTCACCTA+AGG	-	chr4.3:19399455-19399474	None:intergenic	40.0%
!	CCTTTGGTTTCATCCAACAA+AGG	+	chr4.3:19398607-19398626	MS.gene28718:CDS	40.0%
!	GCTGTACAACATTTGGAATG+GGG	-	chr4.3:19398976-19398995	None:intergenic	40.0%
!	TGGCAGCATCTAATTTGTGA+TGG	-	chr4.3:19398458-19398477	None:intergenic	40.0%
!!	TGATGATGGTGTACGTGAAT+GGG	+	chr4.3:19398886-19398905	MS.gene28718:CDS	40.0%
	AAGTCACCAAGAATGCACCA+AGG	-	chr4.3:19399960-19399979	None:intergenic	45.0%
	ACCTGCCATTGCTTCTTTAG+GGG	-	chr4.3:19398740-19398759	None:intergenic	45.0%
	AGCCTGAAACAGAGGATATC+AGG	+	chr4.3:19399320-19399339	MS.gene28718:CDS	45.0%
	AGTCACCAAGAATGCACCAA+GGG	-	chr4.3:19399959-19399978	None:intergenic	45.0%
	ATCAGCCCCTAAAGAAGCAA+TGG	+	chr4.3:19398732-19398751	MS.gene28718:CDS	45.0%
	ATGTTGTTCATGGTCCTTGG+AGG	+	chr4.3:19399847-19399866	MS.gene28718:intron	45.0%
	CCGGGAGAAATGAGATTGAT+AGG	+	chr4.3:19399558-19399577	MS.gene28718:intron	45.0%
	CTAATCAATGTACAGGCCCT+TGG	+	chr4.3:19399940-19399959	MS.gene28718:intron	45.0%
	CTAGCTGAATGTCTCCAAAG+TGG	+	chr4.3:19399370-19399389	MS.gene28718:CDS	45.0%
	CTGATGTTGTTCATGGTCCT+TGG	+	chr4.3:19399844-19399863	MS.gene28718:intron	45.0%
	GACCTGATATCCTCTGTTTC+AGG	-	chr4.3:19399325-19399344	None:intergenic	45.0%
	GACCTGCCATTGCTTCTTTA+GGG	-	chr4.3:19398741-19398760	None:intergenic	45.0%
	GATATCCTCTGTTTCAGGCT+CGG	-	chr4.3:19399320-19399339	None:intergenic	45.0%
	GGAGAAACCTTCGTTCCAAT+TGG	+	chr4.3:19400012-19400031	MS.gene28718:intron	45.0%
	GGTTTCATCCAACAAAGGGT+GGG	+	chr4.3:19398612-19398631	MS.gene28718:intron	45.0%
	GTCATGGAAAGAATCAGTCC+AGG	+	chr4.3:19398522-19398541	MS.gene28718:CDS	45.0%
	TCCCAATACGGTGGACATTA+GGG	-	chr4.3:19398925-19398944	None:intergenic	45.0%
	TCTGTTGATCGTAGTGGTAG+AGG	+	chr4.3:19399738-19399757	MS.gene28718:intron	45.0%
	TGCAGCTGATGGGATTAATG+GGG	+	chr4.3:19399423-19399442	MS.gene28718:CDS	45.0%
	TGGTTCAGATCAACGTTTCG+TGG	-	chr4.3:19398555-19398574	None:intergenic	45.0%
	TGGTTTCATCCAACAAAGGG+TGG	+	chr4.3:19398611-19398630	MS.gene28718:intron	45.0%
!	AAAAGAGGCGCGCCAAAATA+AGG	-	chr4.3:19399775-19399794	None:intergenic	45.0%
!	AATGCGTGGTATTTGGAACC+AGG	+	chr4.3:19400308-19400327	MS.gene28718:intron	45.0%
!	AGCAGCAAGGTTCTCTAAAG+TGG	-	chr4.3:19400204-19400223	None:intergenic	45.0%
!	ATAGGATAGTGGTCTGAGGA+CGG	-	chr4.3:19400227-19400246	None:intergenic	45.0%
!	ATGATGGTGTACGTGAATGG+GGG	+	chr4.3:19398888-19398907	MS.gene28718:CDS	45.0%
!	CTCAGGTTTGGTTGATGTTC+CGG	+	chr4.3:19400067-19400086	MS.gene28718:intron	45.0%
!	GAAAACCATGATGTATCGGC+AGG	+	chr4.3:19399517-19399536	MS.gene28718:intron	45.0%
!	GATGATGGTGTACGTGAATG+GGG	+	chr4.3:19398887-19398906	MS.gene28718:CDS	45.0%
!	GTTTTCGGTACGAAAATGCG+TGG	+	chr4.3:19400294-19400313	MS.gene28718:intron	45.0%
!	TCAGCACCTGTAATGGAAAG+AGG	-	chr4.3:19399280-19399299	None:intergenic	45.0%
!	TCTGCTTGTATTTCTGCTGC+TGG	+	chr4.3:19399211-19399230	MS.gene28718:CDS	45.0%
!!	AGGTTCTCTAAAGTGGCTGA+AGG	-	chr4.3:19400197-19400216	None:intergenic	45.0%
!!	CTGATGATGGTGTACGTGAA+TGG	+	chr4.3:19398885-19398904	MS.gene28718:CDS	45.0%
	AGGTAGCCCGAATGCAATTC+CGG	+	chr4.3:19399599-19399618	MS.gene28718:intron	50.0%
	ATACTACGGGTACCCTGAAG+GGG	+	chr4.3:19399878-19399897	MS.gene28718:intron	50.0%
	CCTAGTTCGGCACTTCAATC+CGG	+	chr4.3:19399632-19399651	MS.gene28718:intron	50.0%
	GATACTACGGGTACCCTGAA+GGG	+	chr4.3:19399877-19399896	MS.gene28718:intron	50.0%
	GCCCTAATGTCCACCGTATT+GGG	+	chr4.3:19398921-19398940	MS.gene28718:CDS	50.0%
	GGACCTGCCATTGCTTCTTT+AGG	-	chr4.3:19398742-19398761	None:intergenic	50.0%
	GTCCCAATACGGTGGACATT+AGG	-	chr4.3:19398926-19398945	None:intergenic	50.0%
	GTCTTGAAGCCACCTGTTAG+TGG	-	chr4.3:19398954-19398973	None:intergenic	50.0%
	GTTGATCGTAGTGGTAGAGG+AGG	+	chr4.3:19399741-19399760	MS.gene28718:intron	50.0%
	TAGTGGCTGCAGTCCCAATA+CGG	-	chr4.3:19398937-19398956	None:intergenic	50.0%
	TCAATCTCATTTCTCCCGGC+AGG	-	chr4.3:19399557-19399576	None:intergenic	50.0%
	TCTGCTCGGTCATACTGACA+AGG	+	chr4.3:19398835-19398854	MS.gene28718:CDS	50.0%
	TCTGGTGCTTGGTATACCTG+CGG	+	chr4.3:19399252-19399271	MS.gene28718:CDS	50.0%
	TGGACCATCATCGAGGACTT+TGG	-	chr4.3:19398478-19398497	None:intergenic	50.0%
	TGGAGGGTTACCCTTTCACT+TGG	+	chr4.3:19400090-19400109	MS.gene28718:intron	50.0%
	TTTAGGTACACCTCGTGCAG+TGG	+	chr4.3:19400121-19400140	MS.gene28718:intron	50.0%
!	AAGCACCAGAGGCCTTTTAG+GGG	-	chr4.3:19399242-19399261	None:intergenic	50.0%
!	AAGTCCTCGATGATGGTCCA+TGG	+	chr4.3:19398478-19398497	MS.gene28718:CDS	50.0%
!	AGCGGTAACTGACTCAGGTT+TGG	+	chr4.3:19400055-19400074	MS.gene28718:intron	50.0%
!	AGGTTTGGTTGATGTTCCGG+TGG	+	chr4.3:19400070-19400089	MS.gene28718:intron	50.0%
!	CAAGCACCAGAGGCCTTTTA+GGG	-	chr4.3:19399243-19399262	None:intergenic	50.0%
!	GGATGGAATTGCAGGGGTAT+AGG	+	chr4.3:19399579-19399598	MS.gene28718:intron	50.0%
!	GGGGTCTTTTGTTACCGTTG+CGG	+	chr4.3:19398812-19398831	MS.gene28718:CDS	50.0%
!	TGATGGTGTACGTGAATGGG+GGG	+	chr4.3:19398889-19398908	MS.gene28718:CDS	50.0%
!	TTCAGGGTACCCGTAGTATC+CGG	-	chr4.3:19399877-19399896	None:intergenic	50.0%
!	TTGGTTGATGTTCCGGTGGA+GGG	+	chr4.3:19400074-19400093	MS.gene28718:intron	50.0%
!	TTTGGTTGATGTTCCGGTGG+AGG	+	chr4.3:19400073-19400092	MS.gene28718:intron	50.0%
!!	TGACGGGTTCCCAATGCTTA+CGG	-	chr4.3:19398368-19398387	None:intergenic	50.0%
	ACAAAGGGTGGGACAAGCCA+TGG	+	chr4.3:19398623-19398642	MS.gene28718:intron	55.0%
	ACGGGTTTCCACGCTTTGAC+GGG	-	chr4.3:19398384-19398403	None:intergenic	55.0%
	ACGTTTCGTGGAACAGCACC+TGG	-	chr4.3:19398543-19398562	None:intergenic	55.0%
	ACTGAGTCGGCCACCCAAAT+TGG	-	chr4.3:19399071-19399090	None:intergenic	55.0%
	AGAGCCATGGACCATCATCG+AGG	-	chr4.3:19398485-19398504	None:intergenic	55.0%
	AGGCGCGCCAAAATAAGGCA+AGG	-	chr4.3:19399770-19399789	None:intergenic	55.0%
	ATTGGGAACCCGTCAAAGCG+TGG	+	chr4.3:19398373-19398392	MS.gene28718:CDS	55.0%
	CAGAAGTGGACGGGAAAGCA+TGG	-	chr4.3:19399195-19399214	None:intergenic	55.0%
	CAGAGCCTGCCGATACATCA+TGG	-	chr4.3:19399525-19399544	None:intergenic	55.0%
	CCTAAAAGGCCTCTGGTGCT+TGG	+	chr4.3:19399241-19399260	MS.gene28718:CDS	55.0%
	CGCCCTAATGTCCACCGTAT+TGG	+	chr4.3:19398920-19398939	MS.gene28718:CDS	55.0%
	CTTCATGAGGCGCACCACTT+TGG	-	chr4.3:19399387-19399406	None:intergenic	55.0%
	GAAGGAAACTGAGAGGCAGC+AGG	-	chr4.3:19399028-19399047	None:intergenic	55.0%
	GAGCGCTTGCCAATAGTGAC+TGG	+	chr4.3:19400159-19400178	MS.gene28718:intron	55.0%
	GATCGTAGTGGTAGAGGAGG+CGG	+	chr4.3:19399744-19399763	MS.gene28718:intron	55.0%
	GCAGGTATACCAAGCACCAG+AGG	-	chr4.3:19399253-19399272	None:intergenic	55.0%
	GCCCCTAAAGAAGCAATGGC+AGG	+	chr4.3:19398736-19398755	MS.gene28718:CDS	55.0%
	GCGGCACCTCTTTCCATTAC+AGG	+	chr4.3:19399271-19399290	MS.gene28718:CDS	55.0%
	GCTGCCAAAGTCCTCGATGA+TGG	+	chr4.3:19398471-19398490	MS.gene28718:CDS	55.0%
	GGATACTACGGGTACCCTGA+AGG	+	chr4.3:19399876-19399895	MS.gene28718:intron	55.0%
	GGCAGGTCCGCTCAAACAAT+GGG	+	chr4.3:19398753-19398772	MS.gene28718:CDS	55.0%
	GTGAAAGGGTAACCCTCCAC+CGG	-	chr4.3:19400089-19400108	None:intergenic	55.0%
	TACTACGGGTACCCTGAAGG+GGG	+	chr4.3:19399879-19399898	MS.gene28718:intron	55.0%
	TAGTATCCGGTGAGCCTCCA+AGG	-	chr4.3:19399864-19399883	None:intergenic	55.0%
	TATGACCGAGCAGACCGCAA+CGG	-	chr4.3:19398829-19398848	None:intergenic	55.0%
	TGAAGATGAGGACGCTGCAC+CGG	-	chr4.3:19400266-19400285	None:intergenic	55.0%
	TGGAGGCTCACCGGATACTA+CGG	+	chr4.3:19399864-19399883	MS.gene28718:intron	55.0%
	TGGCAGGTCCGCTCAAACAA+TGG	+	chr4.3:19398752-19398771	MS.gene28718:CDS	55.0%
	TGTTACCGTTGCGGTCTGCT+CGG	+	chr4.3:19398821-19398840	MS.gene28718:CDS	55.0%
	TTGGGACTGCAGCCACTAAC+AGG	+	chr4.3:19398939-19398958	MS.gene28718:CDS	55.0%
!	ACAGGCCCTTGGTGCATTCT+TGG	+	chr4.3:19399951-19399970	MS.gene28718:intron	55.0%
!	AGCACCAGAGGCCTTTTAGG+GGG	-	chr4.3:19399241-19399260	None:intergenic	55.0%
!	AGGCAAGCGGTAACTGACTC+AGG	+	chr4.3:19400050-19400069	MS.gene28718:intron	55.0%
!	ATCGGCAGGCTCTGATGATC+AGG	+	chr4.3:19399531-19399550	MS.gene28718:intron	55.0%
!	CAAGTTCACGGACTGCCCTT+TGG	+	chr4.3:19398591-19398610	MS.gene28718:CDS	55.0%
!	CCAAGCACCAGAGGCCTTTT+AGG	-	chr4.3:19399244-19399263	None:intergenic	55.0%
!	CCTCTGTTTCAGGCTCGGTA+GGG	-	chr4.3:19399315-19399334	None:intergenic	55.0%
!	CTCTGTTTCAGGCTCGGTAG+GGG	-	chr4.3:19399314-19399333	None:intergenic	55.0%
!	GACGGGTTTCCACGCTTTGA+CGG	-	chr4.3:19398385-19398404	None:intergenic	55.0%
!	TCCTCTGTTTCAGGCTCGGT+AGG	-	chr4.3:19399316-19399335	None:intergenic	55.0%
!	TGGCTTGTCCCACCCTTTGT+TGG	-	chr4.3:19398623-19398642	None:intergenic	55.0%
!!	CCGGATTGAAGTGCCGAACT+AGG	-	chr4.3:19399635-19399654	None:intergenic	55.0%
!!	GTAATGGAAAGAGGTGCCGC+AGG	-	chr4.3:19399271-19399290	None:intergenic	55.0%
!!!	GAGGCGGCCTTGCCTTATTT+TGG	+	chr4.3:19399760-19399779	MS.gene28718:intron	55.0%
	CACCTCGTGCAGTGGAAGAG+CGG	+	chr4.3:19400129-19400148	MS.gene28718:intron	60.0%
	CATGGTCCTTGGAGGCTCAC+CGG	+	chr4.3:19399855-19399874	MS.gene28718:intron	60.0%
	CCCTACCGAGCCTGAAACAG+AGG	+	chr4.3:19399312-19399331	MS.gene28718:CDS	60.0%
	CTCTGATGATCAGGCCTGCC+GGG	+	chr4.3:19399540-19399559	MS.gene28718:intron	60.0%
	GAAGATGAGGACGCTGCACC+GGG	-	chr4.3:19400265-19400284	None:intergenic	60.0%
	GCTCTGATGATCAGGCCTGC+CGG	+	chr4.3:19399539-19399558	MS.gene28718:intron	60.0%
	GGACTGCAGCCACTAACAGG+TGG	+	chr4.3:19398942-19398961	MS.gene28718:CDS	60.0%
	GGAGGCTCACCGGATACTAC+GGG	+	chr4.3:19399865-19399884	MS.gene28718:intron	60.0%
	TGGCTGCAGTCCCAATACGG+TGG	-	chr4.3:19398934-19398953	None:intergenic	60.0%
	TGGTGTACGTGAATGGGGGG+TGG	+	chr4.3:19398892-19398911	MS.gene28718:CDS	60.0%
	TTCGCCCCCTAAAAGGCCTC+TGG	+	chr4.3:19399234-19399253	MS.gene28718:CDS	60.0%
!!	CTGCTGGTTCGCCCCCTAAA+AGG	+	chr4.3:19399227-19399246	MS.gene28718:CDS	60.0%
	AGACGCCTACGTCCCCCTTC+AGG	-	chr4.3:19399894-19399913	None:intergenic	65.0%
	AGCCGCTCTTCCACTGCACG+AGG	-	chr4.3:19400134-19400153	None:intergenic	65.0%
	GACGCCTACGTCCCCCTTCA+GGG	-	chr4.3:19399893-19399912	None:intergenic	65.0%
	GGGACGTAGGCGTCTTGCGT+GGG	+	chr4.3:19399899-19399918	MS.gene28718:intron	65.0%
	GGTACCCTGAAGGGGGACGT+AGG	+	chr4.3:19399886-19399905	MS.gene28718:intron	65.0%
	GGTCTGAGGACGGAGCAGCA+AGG	-	chr4.3:19400217-19400236	None:intergenic	65.0%
	GGGGACGTAGGCGTCTTGCG+TGG	+	chr4.3:19399898-19399917	MS.gene28718:intron	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.3	gene	19398360	19400411	19398360	ID=MS.gene28718
chr4.3	mRNA	19398360	19400411	19398360	ID=MS.gene28718.t1;Parent=MS.gene28718
chr4.3	exon	19398360	19398629	19398360	ID=MS.gene28718.t1.exon1;Parent=MS.gene28718.t1
chr4.3	CDS	19398360	19398629	19398360	ID=cds.MS.gene28718.t1;Parent=MS.gene28718.t1
chr4.3	exon	19398716	19399457	19398716	ID=MS.gene28718.t1.exon2;Parent=MS.gene28718.t1
chr4.3	CDS	19398716	19399457	19398716	ID=cds.MS.gene28718.t1;Parent=MS.gene28718.t1
chr4.3	exon	19400201	19400300	19400201	ID=MS.gene28718.t1.exon3;Parent=MS.gene28718.t1
chr4.3	CDS	19400201	19400300	19400201	ID=cds.MS.gene28718.t1;Parent=MS.gene28718.t1
chr4.3	exon	19400408	19400411	19400408	ID=MS.gene28718.t1.exon4;Parent=MS.gene28718.t1
chr4.3	CDS	19400408	19400411	19400408	ID=cds.MS.gene28718.t1;Parent=MS.gene28718.t1

Gene Sequence

>MS.gene28718

ATGAACCGTAAGCATTGGGAACCCGTCAAAGCGTGGAAACCCGTCAAAAAAGTTACAATTTTGCAATCTGGTGATGACCGTTACCTGTTTCAGTTCCATCACAAATTAGATGCTGCCAAAGTCCTCGATGATGGTCCATGGCTCTATGATAATTTTCATATTGTCATGGAAAGAATCAGTCCAGGTGCTGTTCCACGAAACGTTGATCTGAACCATCTAGATCTTTGGGTACAAGTTCACGGACTGCCCTTTGGTTTCATCCAACAAAGGGTGGGACAAGCCATGGTCAATTTCTCGGAGAAATGAAAGTGTATGATGGTCGTAACTCGGTACACAGTTCATACATGCGTTTGAAGGTCAGAATCAATGTGAATCAGCCCCTAAAGAAGCAATGGCAGGTCCGCTCAAACAATGGGAATTATGTGACTATCCAATTTAAATATGAAAAGTTGGGGGTCTTTTGTTACCGTTGCGGTCTGCTCGGTCATACTGACAAGGTATGTCCTAATTTATTTGAACTTGACTCTGATGATGGTGTACGTGAATGGGGGGTGGAGCTGCGCCCTAATGTCCACCGTATTGGGACTGCAGCCACTAACAGGTGGCTTCAAGACCCCATTCCAAATGTTGTACAGCAAACAAATGGCACTAATGGAGGAACACAACCTGCTGCCTCTCAGTTTCCTTCTGAAAGTTCTTCAAACTTGTCCAATTTGGGTGGCCGACTCAGTGATGTTCATCATGAGATAAATGCTATTAAACAAGGAATTATGGTGTTTCAGAATTCTGCCTTGTCTAAAAGTGGTAAGGTTACTGCTGCTGTTCATCTTAACCATGCTTTCCCGTCCACTTCTGCTTGTATTTCTGCTGCTGGTTCGCCCCCTAAAAGGCCTCTGGTGCTTGGTATACCTGCGGCACCTCTTTCCATTACAGGTGCTGAACATGTTTCTGCCCCTACCGAGCCTGAAACAGAGGATATCAGGTCTGAATTGAAAAAAAGGAAGCGTATGCTAGCTGAATGTCTCCAAAGTGGTGCGCCTCATGAAGAAACTGAATTTTCTTTTGCAGCTGATGGGATTAATGGGGATTTTTCCTTAGGTGAAAATGTTAGTGGGGGGAATGATACTGTAATGAATGTTTGTGAAAATCCTTTGTATGAAAACCATGATGTATCGGCAGGCTCTGATGATCAGGCCTGCCGGGAGAAATGAGATTGATAGGATGGAATTGCAGGGGTATAGGTAGCCCGAATGCAATTCCGGATTTAAAATACCTAGTTCGGCACTTCAATCCGGACCTCTTATTTCTTAGTGAGACGTTAGTCTCCCGTAATAAAATTGAAGATTTACGTTATTTGCTTGGCTTTGATTTTTGTTTTTCTGTTGATCGTAGTGGTAGAGGAGGCGGCCTTGCCTTATTTTGGCGCGCCTCTTTTAATTGTAATATTGTAAACTATTCCAATAATCATATCTCTGTTGATGTTACTGATGTTGTTCATGGTCCTTGGAGGCTCACCGGATACTACGGGTACCCTGAAGGGGGACGTAGGCGTCTTGCGTGGGATTTCCTTCGACAATTATCTAATCAATGTACAGGCCCTTGGTGCATTCTTGGTGACTTTAATGATTTAATGGATGCTAGTGAAAAAAGAGGGAGAAACCTTCGTTCCAATTGGTTAATTAATGGCTTTAGGCAAGCGGTAACTGACTCAGGTTTGGTTGATGTTCCGGTGGAGGGTTACCCTTTCACTTGGTTCAAGAGTTTAGGTACACCTCGTGCAGTGGAAGAGCGGCTTGATAGAGCGCTTGCCAATAGTGACTGGTTTAATCTTTTTCCTTCAGCCACTTTAGAGAACCTTGCTGCTCCGTCCTCAGACCACTATCCTATTCTTTTCAATAGAAACCCGGTGCAGCGTCCTCATCTTCATAAACGTAGTTTTCGGTACGAAAATGCGTGGTATTTGGAACCAGGTTTCAAAGATTTAGTAGCTACTGCTTGGCAGCAACATAATGTTTACTCAGTGATGCCTAAGTTAACTCATTGTGCAGCTGA

Protein sequence

>MS.gene28718.t1

MNRKHWEPVKAWKPVKKVTILQSGDDRYLFQFHHKLDAAKVLDDGPWLYDNFHIVMERISPGAVPRNVDLNHLDLWVQVHGLPFGFIQQRVRINVNQPLKKQWQVRSNNGNYVTIQFKYEKLGVFCYRCGLLGHTDKVCPNLFELDSDDGVREWGVELRPNVHRIGTAATNRWLQDPIPNVVQQTNGTNGGTQPAASQFPSESSSNLSNLGGRLSDVHHEINAIKQGIMVFQNSALSKSGKVTAAVHLNHAFPSTSACISAAGSPPKRPLVLGIPAAPLSITGAEHVSAPTEPETEDIRSELKKRKRMLAECLQSGAPHEETEFSFAADGINGDFSLATLENLAAPSSDHYPILFNRNPVQRPHLHKRSFR