AlfalfaGEDB | MS.gene21066

M. sativa cultivar XinJiangDaYe / MS.gene21066

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21066.t1	XP_024630605.1	71.5	253	66	1	84	336	48	294	7.30E-102	380.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21066.t1	O04244	26.5	136	96	2	83	216	6	139	5.7e-10	67.0

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21066.t1	A0A2K3L2W5	44.4	378	183	6	6	378	125	480	2.9e-84	322.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene025873	MS.gene21066	PPI
MS.gene012019	MS.gene21066	PPI
MS.gene06164	MS.gene21066	PPI
MS.gene013676	MS.gene21066	PPI
MS.gene05358	MS.gene21066	PPI
MS.gene95561	MS.gene21066	PPI
MS.gene074257	MS.gene21066	PPI
MS.gene065627	MS.gene21066	PPI
MS.gene21066	MS.gene008658	PPI
MS.gene010890	MS.gene21066	PPI
MS.gene018745	MS.gene21066	PPI
MS.gene000629	MS.gene21066	PPI
MS.gene65546	MS.gene21066	PPI
MS.gene09023	MS.gene21066	PPI
MS.gene059269	MS.gene21066	PPI
MS.gene000506	MS.gene21066	PPI
MS.gene74639	MS.gene21066	PPI
MS.gene032299	MS.gene21066	PPI
MS.gene03936	MS.gene21066	PPI
MS.gene038098	MS.gene21066	PPI
MS.gene56991	MS.gene21066	PPI
MS.gene60059	MS.gene21066	PPI
MS.gene85538	MS.gene21066	PPI
MS.gene062511	MS.gene21066	PPI
MS.gene22111	MS.gene21066	PPI
MS.gene28545	MS.gene21066	PPI
MS.gene28718	MS.gene21066	PPI
MS.gene055560	MS.gene21066	PPI
MS.gene29321	MS.gene21066	PPI
MS.gene34618	MS.gene21066	PPI
MS.gene59460	MS.gene21066	PPI
MS.gene038757	MS.gene21066	PPI
MS.gene064966	MS.gene21066	PPI
MS.gene069624	MS.gene21066	PPI
MS.gene016632	MS.gene21066	PPI
MS.gene002590	MS.gene21066	PPI
MS.gene21066	MS.gene74639	PPI
MS.gene21066	MS.gene071133	PPI
MS.gene000640	MS.gene21066	PPI
MS.gene40828	MS.gene21066	PPI
MS.gene43924	MS.gene21066	PPI
MS.gene046089	MS.gene21066	PPI
MS.gene047954	MS.gene21066	PPI
MS.gene77990	MS.gene21066	PPI
MS.gene036423	MS.gene21066	PPI
MS.gene21066	MS.gene37955	PPI
MS.gene054652	MS.gene21066	PPI
MS.gene21066	MS.gene84790	PPI
MS.gene013644	MS.gene21066	PPI
MS.gene84790	MS.gene21066	PPI
MS.gene72520	MS.gene21066	PPI
MS.gene61651	MS.gene21066	PPI
MS.gene66651	MS.gene21066	PPI
MS.gene24278	MS.gene21066	PPI
MS.gene35202	MS.gene21066	PPI
MS.gene025158	MS.gene21066	PPI
MS.gene051449	MS.gene21066	PPI
MS.gene61499	MS.gene21066	PPI
MS.gene068629	MS.gene21066	PPI
MS.gene019001	MS.gene21066	PPI
MS.gene63743	MS.gene21066	PPI
MS.gene63150	MS.gene21066	PPI
MS.gene48477	MS.gene21066	PPI
MS.gene21066	MS.gene027994	PPI
MS.gene045875	MS.gene21066	PPI
MS.gene54051	MS.gene21066	PPI
MS.gene038394	MS.gene21066	PPI
MS.gene03149	MS.gene21066	PPI
MS.gene30136	MS.gene21066	PPI
MS.gene008861	MS.gene21066	PPI
MS.gene024405	MS.gene21066	PPI
MS.gene73261	MS.gene21066	PPI
MS.gene012748	MS.gene21066	PPI
MS.gene024297	MS.gene21066	PPI
MS.gene83979	MS.gene21066	PPI
MS.gene45083	MS.gene21066	PPI
MS.gene92517	MS.gene21066	PPI
MS.gene85295	MS.gene21066	PPI
MS.gene057934	MS.gene21066	PPI
MS.gene60390	MS.gene21066	PPI
MS.gene47792	MS.gene21066	PPI
MS.gene46948	MS.gene21066	PPI
MS.gene21066	MS.gene61651	PPI
MS.gene98471	MS.gene21066	PPI
MS.gene028714	MS.gene21066	PPI
MS.gene049610	MS.gene21066	PPI
MS.gene060718	MS.gene21066	PPI
MS.gene025401	MS.gene21066	PPI
MS.gene93396	MS.gene21066	PPI
MS.gene21066	MS.gene88393	PPI
MS.gene21066	MS.gene025987	PPI
MS.gene05576	MS.gene21066	PPI
MS.gene52349	MS.gene21066	PPI
MS.gene05231	MS.gene21066	PPI
MS.gene98467	MS.gene21066	PPI
MS.gene46353	MS.gene21066	PPI
MS.gene010656	MS.gene21066	PPI
MS.gene42010	MS.gene21066	PPI
MS.gene40469	MS.gene21066	PPI
MS.gene041264	MS.gene21066	PPI
MS.gene41979	MS.gene21066	PPI
MS.gene024522	MS.gene21066	PPI
MS.gene000379	MS.gene21066	PPI
MS.gene048342	MS.gene21066	PPI
MS.gene64130	MS.gene21066	PPI
MS.gene05319	MS.gene21066	PPI
MS.gene88393	MS.gene21066	PPI
MS.gene050679	MS.gene21066	PPI
MS.gene21066	MS.gene015093	PPI
MS.gene20246	MS.gene21066	PPI
MS.gene055223	MS.gene21066	PPI
MS.gene21066	MS.gene000379	PPI
MS.gene016719	MS.gene21066	PPI
MS.gene010290	MS.gene21066	PPI
MS.gene053747	MS.gene21066	PPI
MS.gene21066	MS.gene08606	PPI
MS.gene65471	MS.gene21066	PPI
MS.gene061850	MS.gene21066	PPI
MS.gene21066	MS.gene000640	PPI
MS.gene21066	MS.gene09517	PPI
MS.gene26433	MS.gene21066	PPI
MS.gene34463	MS.gene21066	PPI
MS.gene048088	MS.gene21066	PPI
MS.gene008658	MS.gene21066	PPI
MS.gene018474	MS.gene21066	PPI
MS.gene22730	MS.gene21066	PPI
MS.gene003264	MS.gene21066	PPI
MS.gene073582	MS.gene21066	PPI
MS.gene042559	MS.gene21066	PPI
MS.gene21066	MS.gene62249	PPI
MS.gene058889	MS.gene21066	PPI
MS.gene98616	MS.gene21066	PPI
MS.gene071133	MS.gene21066	PPI
MS.gene53904	MS.gene21066	PPI
MS.gene08708	MS.gene21066	PPI
MS.gene09517	MS.gene21066	PPI
MS.gene69586	MS.gene21066	PPI
MS.gene042027	MS.gene21066	PPI
MS.gene85308	MS.gene21066	PPI
MS.gene69490	MS.gene21066	PPI
MS.gene043140	MS.gene21066	PPI
MS.gene015248	MS.gene21066	PPI
MS.gene012889	MS.gene21066	PPI
MS.gene80362	MS.gene21066	PPI
MS.gene21066	MS.gene60390	PPI
MS.gene44083	MS.gene21066	PPI
MS.gene37537	MS.gene21066	PPI
MS.gene013193	MS.gene21066	PPI
MS.gene04970	MS.gene21066	PPI
MS.gene08106	MS.gene21066	PPI
MS.gene51799	MS.gene21066	PPI
MS.gene58419	MS.gene21066	PPI
MS.gene027994	MS.gene21066	PPI
MS.gene96163	MS.gene21066	PPI
MS.gene08713	MS.gene21066	PPI
MS.gene65474	MS.gene21066	PPI
MS.gene23521	MS.gene21066	PPI
MS.gene29406	MS.gene21066	PPI
MS.gene86275	MS.gene21066	PPI
MS.gene98127	MS.gene21066	PPI
MS.gene87529	MS.gene21066	PPI
MS.gene04134	MS.gene21066	PPI
MS.gene062067	MS.gene21066	PPI
MS.gene21066	MS.gene025158	PPI
MS.gene37955	MS.gene21066	PPI
MS.gene57044	MS.gene21066	PPI
MS.gene054508	MS.gene21066	PPI
MS.gene89815	MS.gene21066	PPI
MS.gene017586	MS.gene21066	PPI
MS.gene038676	MS.gene21066	PPI
MS.gene98756	MS.gene21066	PPI
MS.gene71637	MS.gene21066	PPI
MS.gene042651	MS.gene21066	PPI
MS.gene21066	MS.gene043140	PPI
MS.gene21066	MS.gene03149	PPI
MS.gene037049	MS.gene21066	PPI
MS.gene034643	MS.gene21066	PPI
MS.gene044729	MS.gene21066	PPI
MS.gene037634	MS.gene21066	PPI
MS.gene24070	MS.gene21066	PPI
MS.gene71588	MS.gene21066	PPI
MS.gene60266	MS.gene21066	PPI
MS.gene04749	MS.gene21066	PPI
MS.gene21066	MS.gene24278	PPI
MS.gene21066	MS.gene59460	PPI
MS.gene071784	MS.gene21066	PPI
MS.gene027169	MS.gene21066	PPI
MS.gene061579	MS.gene21066	PPI
MS.gene039692	MS.gene21066	PPI
MS.gene76420	MS.gene21066	PPI
MS.gene071320	MS.gene21066	PPI
MS.gene022929	MS.gene21066	PPI
MS.gene42903	MS.gene21066	PPI
MS.gene81712	MS.gene21066	PPI
MS.gene026680	MS.gene21066	PPI
MS.gene021555	MS.gene21066	PPI
MS.gene048747	MS.gene21066	PPI
MS.gene22200	MS.gene21066	PPI
MS.gene019165	MS.gene21066	PPI
MS.gene055546	MS.gene21066	PPI
MS.gene26038	MS.gene21066	PPI
MS.gene08606	MS.gene21066	PPI
MS.gene49057	MS.gene21066	PPI
MS.gene21066	MS.gene44144	PPI
MS.gene073573	MS.gene21066	PPI
MS.gene031967	MS.gene21066	PPI
MS.gene053373	MS.gene21066	PPI
MS.gene34812	MS.gene21066	PPI
MS.gene02896	MS.gene21066	PPI
MS.gene063214	MS.gene21066	PPI
MS.gene55682	MS.gene21066	PPI
MS.gene97410	MS.gene21066	PPI
MS.gene32415	MS.gene21066	PPI
MS.gene009213	MS.gene21066	PPI
MS.gene060788	MS.gene21066	PPI
MS.gene035626	MS.gene21066	PPI
MS.gene067340	MS.gene21066	PPI
MS.gene65623	MS.gene21066	PPI
MS.gene27329	MS.gene21066	PPI
MS.gene71675	MS.gene21066	PPI
MS.gene019177	MS.gene21066	PPI
MS.gene69495	MS.gene21066	PPI
MS.gene049512	MS.gene21066	PPI
MS.gene038623	MS.gene21066	PPI
MS.gene55209	MS.gene21066	PPI
MS.gene05357	MS.gene21066	PPI
MS.gene032311	MS.gene21066	PPI
MS.gene64178	MS.gene21066	PPI
MS.gene004417	MS.gene21066	PPI
MS.gene030090	MS.gene21066	PPI
MS.gene96174	MS.gene21066	PPI
MS.gene018196	MS.gene21066	PPI
MS.gene86849	MS.gene21066	PPI
MS.gene50392	MS.gene21066	PPI
MS.gene072852	MS.gene21066	PPI
MS.gene57019	MS.gene21066	PPI
MS.gene21066	MS.gene018474	PPI
MS.gene41393	MS.gene21066	PPI
MS.gene21066	MS.gene86868	PPI
MS.gene39956	MS.gene21066	PPI
MS.gene021968	MS.gene21066	PPI
MS.gene09589	MS.gene21066	PPI
MS.gene44144	MS.gene21066	PPI
MS.gene43393	MS.gene21066	PPI
MS.gene057828	MS.gene21066	PPI
MS.gene34197	MS.gene21066	PPI
MS.gene008275	MS.gene21066	PPI
MS.gene66650	MS.gene21066	PPI
MS.gene005120	MS.gene21066	PPI
MS.gene86868	MS.gene21066	PPI
MS.gene048072	MS.gene21066	PPI
MS.gene060386	MS.gene21066	PPI
MS.gene025987	MS.gene21066	PPI
MS.gene21066	MS.gene61499	PPI
MS.gene05436	MS.gene21066	PPI
MS.gene059736	MS.gene21066	PPI
MS.gene70932	MS.gene21066	PPI
MS.gene35175	MS.gene21066	PPI
MS.gene62249	MS.gene21066	PPI
MS.gene81194	MS.gene21066	PPI
MS.gene025675	MS.gene21066	PPI
MS.gene069786	MS.gene21066	PPI
MS.gene021965	MS.gene21066	PPI
MS.gene055683	MS.gene21066	PPI
MS.gene042614	MS.gene21066	PPI
MS.gene41643	MS.gene21066	PPI
MS.gene013890	MS.gene21066	PPI
MS.gene26844	MS.gene21066	PPI
MS.gene060773	MS.gene21066	PPI
MS.gene021647	MS.gene21066	PPI
MS.gene96109	MS.gene21066	PPI
MS.gene69584	MS.gene21066	PPI
MS.gene048706	MS.gene21066	PPI
MS.gene015093	MS.gene21066	PPI
MS.gene007022	MS.gene21066	PPI
MS.gene009121	MS.gene21066	PPI
MS.gene94654	MS.gene21066	PPI
MS.gene024841	MS.gene21066	PPI
MS.gene51804	MS.gene21066	PPI
MS.gene87845	MS.gene21066	PPI
MS.gene36069	MS.gene21066	PPI
MS.gene21066	MS.gene022929	PPI
MS.gene21066	MS.gene007022	PPI
MS.gene95960	MS.gene21066	PPI
MS.gene61452	MS.gene21066	PPI
MS.gene83772	MS.gene21066	PPI
MS.gene008927	MS.gene21066	PPI
MS.gene016102	MS.gene21066	PPI
MS.gene46941	MS.gene21066	PPI
MS.gene045614	MS.gene21066	PPI
MS.gene96541	MS.gene21066	PPI
MS.gene049561	MS.gene21066	PPI
MS.gene000634	MS.gene21066	PPI
MS.gene009781	MS.gene21066	PPI
MS.gene21066	MS.gene048342	PPI
MS.gene63762	MS.gene21066	PPI
MS.gene30208	MS.gene21066	PPI
MS.gene028716	MS.gene21066	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21066.t1	MTR_3g053920	45.640	344	120	3	1	344	1	277	2.50e-94	285
MS.gene21066.t1	MTR_2g061500	69.767	129	39	0	1	129	1	129	2.22e-64	203
MS.gene21066.t1	MTR_4g103530	67.742	93	30	0	55	147	1	93	8.05e-42	146
MS.gene21066.t1	MTR_7g011710	52.000	125	55	2	55	179	1	120	9.91e-36	129
MS.gene21066.t1	MTR_6g033215	32.584	178	116	2	26	199	117	294	9.00e-35	130
MS.gene21066.t1	MTR_0010s0370	33.918	171	109	2	11	177	7	177	1.33e-28	111
MS.gene21066.t1	MTR_0010s0350	33.918	171	109	2	11	177	7	177	1.33e-28	111
MS.gene21066.t1	MTR_1g036720	33.654	104	67	1	11	112	7	110	3.18e-13	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene21066.t1	AT2G41590	30.282	142	95	2	77	216	80	219	5.19e-18	85.5
MS.gene21066.t1	AT2G17920	29.054	148	100	3	72	216	74	219	8.75e-18	84.0
MS.gene21066.t1	AT3G57587	28.873	142	97	2	77	216	80	219	1.74e-17	84.0
MS.gene21066.t1	AT5G18636	26.519	181	126	4	77	255	80	255	3.06e-16	80.1
MS.gene21066.t1	AT5G25200	28.873	142	97	2	77	216	80	219	6.18e-16	79.3
MS.gene21066.t1	AT5G36228	28.395	162	108	4	72	227	74	233	2.02e-15	77.8
MS.gene21066.t1	AT2G02103	28.873	142	97	2	77	216	80	219	2.20e-15	77.8
MS.gene21066.t1	AT2G13450	25.824	182	131	2	36	215	39	218	2.26e-15	77.8
MS.gene21066.t1	AT3G31430	25.455	220	145	6	9	215	98	311	1.78e-14	74.7
MS.gene21066.t1	AT3G47920	26.761	142	100	2	77	216	9	148	1.21e-13	71.6
MS.gene21066.t1	AT2G01050	22.388	268	190	7	35	292	80	339	1.58e-12	69.7

Find 135 sgRNAs with CRISPR-Local

Find 150 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTCTTCAAACTTGTCCAATT+TGG	0.111128	1.4:-68454373	MS.gene21066:CDS
GGACCTGCCATTGCTTCTTT+AGG	0.166134	1.4:+68454688	None:intergenic
TAGAGGTGCCGCAGGCCTTT+GGG	0.212433	1.4:+68454185	None:intergenic
GACCTGATATCCTCTGTTTC+AGG	0.218469	1.4:+68454123	None:intergenic
TTTGCAGCTGATGGGATTAA+TGG	0.226048	1.4:-68454024	MS.gene21066:CDS
ATCTGAACCATCTAGATCTT+TGG	0.231408	1.4:-68454863	MS.gene21066:CDS
TTGTCTGATCGGTCGTATTT+TGG	0.233850	1.4:-68455108	MS.gene21066:CDS
GACCTGCCATTGCTTCTTTA+GGG	0.246581	1.4:+68454689	None:intergenic
TTTCCTTAGGTGAAAATGTT+AGG	0.248157	1.4:-68453996	MS.gene21066:CDS
TCTGCTTGTATTTCTGTTGC+TGG	0.258349	1.4:-68454216	MS.gene21066:CDS
TGCTATTAAACAAGGAATTA+TGG	0.273925	1.4:-68454316	MS.gene21066:CDS
GTCCCAATACGGTGGACATT+AGG	0.291656	1.4:+68454504	None:intergenic
ATTTGTGATGGAACTGAAAT+AGG	0.304567	1.4:+68454985	None:intergenic
TCAGGTCTGAACTGAGAAAA+AGG	0.310795	1.4:-68454107	MS.gene21066:CDS
TCAAGGATCTGGGTCACTTC+AGG	0.314256	1.4:+68455134	None:intergenic
GAGTCAAGTTCAAATAAATT+AGG	0.315354	1.4:+68454564	None:intergenic
ATAGAGGTGCCGCAGGCCTT+TGG	0.318201	1.4:+68454184	None:intergenic
TCTTCAAACTTGTCCAATTT+GGG	0.322600	1.4:-68454372	MS.gene21066:CDS
TTGGTTTGCTGTACAACATT+TGG	0.324252	1.4:+68454447	None:intergenic
TAGCTTGTCCCACCCTTTGT+TGG	0.339598	1.4:+68454808	None:intergenic
ACTGAGTCGGCCACCCAAAT+TGG	0.348545	1.4:+68454359	None:intergenic
TTGCAGCTGATGGGATTAAT+GGG	0.358856	1.4:-68454023	MS.gene21066:CDS
TCAGACAATGGTCAAGGATC+TGG	0.364250	1.4:+68455123	None:intergenic
GCCCTAATGTCCACCGTATT+GGG	0.371713	1.4:-68454506	MS.gene21066:CDS
CAATTTAAATATGAAAAGTT+GGG	0.374919	1.4:-68454636	MS.gene21066:CDS
TGCTGTACAACATTTGGAAT+GGG	0.383899	1.4:+68454453	None:intergenic
GCGGCACCTCTATCCATTAC+AGG	0.384877	1.4:-68454174	MS.gene21066:CDS
TGTTCCTCCATTAGTGCCAT+TGG	0.387875	1.4:+68454428	None:intergenic
TTTGACGGGTTTCCAGGCTT+TGG	0.388781	1.4:+68455043	None:intergenic
CCAATTTAAATATGAAAAGT+TGG	0.392300	1.4:-68454637	MS.gene21066:CDS
CAGCAAACCAATGGCACTAA+TGG	0.392365	1.4:-68454435	MS.gene21066:CDS
TGCCCTAATGTCCACCGTAT+TGG	0.392500	1.4:-68454507	MS.gene21066:CDS
TCTGAACCATCTAGATCTTT+GGG	0.396460	1.4:-68454862	MS.gene21066:CDS
TAGGACATACCTTGTCAGTA+TGG	0.399139	1.4:+68454583	None:intergenic
CTTCCTGAGGCGCGCCACTT+TGG	0.399985	1.4:+68454061	None:intergenic
TGGCAGGTCCGCTCAAACAA+TGG	0.400450	1.4:-68454675	MS.gene21066:CDS
TTCCTTAGGTGAAAATGTTA+GGG	0.410592	1.4:-68453995	MS.gene21066:CDS
TCCCAATACGGTGGACATTA+GGG	0.412861	1.4:+68454505	None:intergenic
CAAGCTATTGGTCAGTTTCT+CGG	0.423729	1.4:-68454792	MS.gene21066:CDS
AGAGGTGCCGCAGGCCTTTG+GGG	0.430691	1.4:+68454186	None:intergenic
TGATGATGGTGTACGTGAAT+GGG	0.434942	1.4:-68454541	MS.gene21066:CDS
CATACATGCGTTTGAAGGTC+AGG	0.434990	1.4:-68454728	MS.gene21066:CDS
CAAGTTCACGGACTGCCCTT+TGG	0.437581	1.4:-68454837	MS.gene21066:CDS
TTGGGACTACAGCCACTAAC+AGG	0.444958	1.4:-68454488	MS.gene21066:CDS
GTCTTGAAGCCACCTGTTAG+TGG	0.448129	1.4:+68454476	None:intergenic
CAAAGGGTGGGACAAGCTAT+TGG	0.452402	1.4:-68454804	MS.gene21066:CDS
TCTCCAAAGTGGCGCGCCTC+AGG	0.453068	1.4:-68454064	MS.gene21066:CDS
AGCCTGAAACAGAGGATATC+AGG	0.456205	1.4:-68454125	MS.gene21066:CDS
TTTGAACTTGACTCTGATGA+TGG	0.467042	1.4:-68454555	MS.gene21066:CDS
GGCCTTACCTTAGCTGCTGA+AGG	0.470138	1.4:-68455182	MS.gene21066:CDS
ATGTTTCGTGGGACAACACC+TGG	0.470559	1.4:+68454888	None:intergenic
TGTTACCGTTGCGGTCTGCT+TGG	0.474269	1.4:-68454606	MS.gene21066:CDS
CTTTGGTTTCATCCAACAAA+GGG	0.475670	1.4:-68454820	MS.gene21066:CDS
GGCAGGTCCGCTCAAACAAT+GGG	0.477547	1.4:-68454674	MS.gene21066:CDS
CAAGGATCTGGGTCACTTCA+GGG	0.477893	1.4:+68455135	None:intergenic
TCCTCTGTTTCAGGCTCGGT+AGG	0.485474	1.4:+68454132	None:intergenic
AACATGTTCAGCACCTGTAA+TGG	0.488119	1.4:+68454161	None:intergenic
TAGTTCATACATGCGTTTGA+AGG	0.489504	1.4:-68454733	MS.gene21066:CDS
CTGATGATGGTGTACGTGAA+TGG	0.493705	1.4:-68454542	MS.gene21066:CDS
ATCCTTGACCATTGTCTGAT+CGG	0.498404	1.4:-68455119	MS.gene21066:CDS
CAGACAATGGTCAAGGATCT+GGG	0.499963	1.4:+68455124	None:intergenic
AGGGTTATCAGCAAGGTTCA+AGG	0.499993	1.4:+68455154	None:intergenic
GATCTTTGGGTACAAGTTCA+CGG	0.501951	1.4:-68454849	MS.gene21066:CDS
AATTCTGCCTTGTCTAAAAG+TGG	0.503637	1.4:-68454285	MS.gene21066:CDS
CCTCTGTTTCAGGCTCGGTA+GGG	0.506009	1.4:+68454133	None:intergenic
TTGCTGTACAACATTTGGAA+TGG	0.506504	1.4:+68454452	None:intergenic
GGTCAAACCTTCAGCAGCTA+AGG	0.515977	1.4:+68455175	None:intergenic
GAAAATTATCATAGAGCCAT+GGG	0.517864	1.4:+68454934	None:intergenic
TGCCTTGTCTAAAAGTGGTA+AGG	0.519126	1.4:-68454280	MS.gene21066:CDS
TGGACAGGAAAGCATGGTTA+AGG	0.519178	1.4:+68454241	None:intergenic
ATTCAAAGCTTGAGGATCCA+TGG	0.519762	1.4:+68455211	None:intergenic
TGGCAGCATCTAATTTGTGA+TGG	0.521268	1.4:+68454973	None:intergenic
AAGTTTGAAGAACTTTCAGC+AGG	0.521273	1.4:+68454384	None:intergenic
TGGGCCATCATCGAGGACTT+TGG	0.521891	1.4:+68454953	None:intergenic
AACCTTCAGCAGCTAAGGTA+AGG	0.523925	1.4:+68455180	None:intergenic
CTCTGTTTCAGGCTCGGTAG+GGG	0.525207	1.4:+68454134	None:intergenic
ACCTGCCATTGCTTCTTTAG+GGG	0.525977	1.4:+68454690	None:intergenic
ACTTTCAGCAGGAAACTGAT+AGG	0.527253	1.4:+68454395	None:intergenic
AAATACAAGCAGAAGTGGAC+AGG	0.531561	1.4:+68454226	None:intergenic
TCAGCACCTGTAATGGATAG+AGG	0.534589	1.4:+68454168	None:intergenic
GATATCCTCTGTTTCAGGCT+CGG	0.539302	1.4:+68454128	None:intergenic
GTGAGCGCATGTCCAAAGCC+TGG	0.539407	1.4:-68455055	MS.gene21066:CDS
GAAAATTCAGTTTCTTCCTG+AGG	0.544593	1.4:+68454048	None:intergenic
CCTTTGGTTTCATCCAACAA+AGG	0.546455	1.4:-68454821	MS.gene21066:CDS
GATGATGGTGTACGTGAATG+GGG	0.546601	1.4:-68454540	MS.gene21066:CDS
AATTTAAATATGAAAAGTTG+GGG	0.547584	1.4:-68454635	MS.gene21066:CDS
CTTGTACCCAAAGATCTAGA+TGG	0.548240	1.4:+68454856	None:intergenic
CATAATTCCCATTGTTTGAG+CGG	0.549445	1.4:+68454667	None:intergenic
TGAAAATTATCATAGAGCCA+TGG	0.551305	1.4:+68454933	None:intergenic
GCTGCCAAAGTCCTCGATGA+TGG	0.551575	1.4:-68454957	MS.gene21066:CDS
GAGGTGCCGCAGGCCTTTGG+GGG	0.555148	1.4:+68454187	None:intergenic
TGGTTTCATCCAACAAAGGG+TGG	0.556650	1.4:-68454817	MS.gene21066:CDS
CCCTACCGAGCCTGAAACAG+AGG	0.565438	1.4:-68454133	MS.gene21066:CDS
CTTTGTTGGATGAAACCAAA+GGG	0.565476	1.4:+68454822	None:intergenic
AACAGAAATACAAGCAGAAG+TGG	0.565540	1.4:+68454221	None:intergenic
GCAGGAAACTGATAGGCAGC+AGG	0.566252	1.4:+68454402	None:intergenic
CCATCAAAATTCAAAGCTTG+AGG	0.567633	1.4:+68455203	None:intergenic
GCCCCTAAAGAAGCAATGGC+AGG	0.569503	1.4:-68454691	MS.gene21066:CDS
TCAAACTTGTCCAATTTGGG+TGG	0.570842	1.4:-68454369	MS.gene21066:CDS
GGTTCAGATCAATGTTTCGT+GGG	0.574709	1.4:+68454877	None:intergenic
TCACTTCAGGGTTATCAGCA+AGG	0.578957	1.4:+68455147	None:intergenic
TAGTGGCTGTAGTCCCAATA+CGG	0.584759	1.4:+68454493	None:intergenic
GGTTTCATCCAACAAAGGGT+GGG	0.584771	1.4:-68454816	MS.gene21066:CDS
ATGATGGTGTACGTGAATGG+GGG	0.589038	1.4:-68454539	MS.gene21066:CDS
TGGTTCAGATCAATGTTTCG+TGG	0.593017	1.4:+68454876	None:intergenic
ATCAGCCCCTAAAGAAGCAA+TGG	0.593819	1.4:-68454695	MS.gene21066:CDS
TGCAGCTGATGGGATTAATG+GGG	0.596716	1.4:-68454022	MS.gene21066:CDS
GCTGTACAACATTTGGAATG+GGG	0.599803	1.4:+68454454	None:intergenic
CAAACCAATGGCACTAATGG+AGG	0.607950	1.4:-68454432	MS.gene21066:CDS
GTCGTGGAAAGAATCAGTCC+AGG	0.609256	1.4:-68454906	MS.gene21066:CDS
GACCGATCAGACAATGGTCA+AGG	0.614534	1.4:+68455117	None:intergenic
GAGATAAATGCTATTAAACA+AGG	0.615670	1.4:-68454324	MS.gene21066:CDS
CTTAGGTGAAAATGTTAGGG+GGG	0.617989	1.4:-68453992	MS.gene21066:CDS
GTAATGGATAGAGGTGCCGC+AGG	0.618670	1.4:+68454177	None:intergenic
CAGAAGTGGACAGGAAAGCA+TGG	0.619846	1.4:+68454235	None:intergenic
AAGTCCTCGATGATGGCCCA+TGG	0.622235	1.4:-68454950	MS.gene21066:CDS
CATGATGAACAGCACTGAGT+CGG	0.625770	1.4:+68454346	None:intergenic
TGATGGTGTACGTGAATGGG+GGG	0.627050	1.4:-68454538	MS.gene21066:CDS
AATGTTGTACAGCAAACCAA+TGG	0.631109	1.4:-68454444	MS.gene21066:CDS
TTAGGTGAAAATGTTAGGGG+GGG	0.631422	1.4:-68453991	MS.gene21066:CDS
GATGGAACTGAAATAGGTAG+CGG	0.636283	1.4:+68454991	None:intergenic
GGAGAAATGAAAGTGTATGA+TGG	0.645041	1.4:-68454771	MS.gene21066:CDS
CTAAGTGAATGTCTCCAAAG+TGG	0.646192	1.4:-68454075	MS.gene21066:CDS
TCCTTAGGTGAAAATGTTAG+GGG	0.649138	1.4:-68453994	MS.gene21066:CDS
TATTAAACAAGGAATTATGG+TGG	0.651391	1.4:-68454313	MS.gene21066:CDS
TCTGCTTGGCCATACTGACA+AGG	0.652759	1.4:-68454592	MS.gene21066:CDS
CCTTAGGTGAAAATGTTAGG+GGG	0.660457	1.4:-68453993	MS.gene21066:CDS
GGACTACAGCCACTAACAGG+TGG	0.667366	1.4:-68454485	MS.gene21066:CDS
TATGGCCAAGCAGACCGCAA+CGG	0.669030	1.4:+68454601	None:intergenic
CCTTTGTTGGATGAAACCAA+AGG	0.680480	1.4:+68454821	None:intergenic
AGTGTATGATGGCTGTAACT+CGG	0.682725	1.4:-68454760	MS.gene21066:CDS
AAATACGACCGATCAGACAA+TGG	0.686548	1.4:+68455111	None:intergenic
ATTTAAATATGAAAAGTTGG+GGG	0.694275	1.4:-68454634	MS.gene21066:CDS
TGGCTGTAGTCCCAATACGG+TGG	0.709193	1.4:+68454496	None:intergenic
AGAGCCATGGGCCATCATCG+AGG	0.713353	1.4:+68454946	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AATTTAAATATGAAAAGTTG+GGG	-	chr1.4:68454552-68454571	MS.gene21066:CDS	15.0%
!!	CAATTTAAATATGAAAAGTT+GGG	-	chr1.4:68454551-68454570	MS.gene21066:CDS	15.0%
!!	ATTTAAATATGAAAAGTTGG+GGG	-	chr1.4:68454553-68454572	MS.gene21066:CDS	20.0%
!!	CCAATTTAAATATGAAAAGT+TGG	-	chr1.4:68454550-68454569	MS.gene21066:CDS	20.0%
!!!	AAAATTGTAACTTTTTTGAC+GGG	+	chr1.4:68454161-68454180	None:intergenic	20.0%
!!!	AAAGTTACAATTTTGCAATA+TGG	-	chr1.4:68454167-68454186	MS.gene21066:CDS	20.0%
!!!	CAAAATTGTAACTTTTTTGA+CGG	+	chr1.4:68454162-68454181	None:intergenic	20.0%
!!!	CCAACTTTTCATATTTAAAT+TGG	+	chr1.4:68454553-68454572	None:intergenic	20.0%
!	GAGATAAATGCTATTAAACA+AGG	-	chr1.4:68454863-68454882	MS.gene21066:CDS	25.0%
!	GAGTCAAGTTCAAATAAATT+AGG	+	chr1.4:68454626-68454645	None:intergenic	25.0%
!	TATTAAACAAGGAATTATGG+TGG	-	chr1.4:68454874-68454893	MS.gene21066:CDS	25.0%
!	TGCTATTAAACAAGGAATTA+TGG	-	chr1.4:68454871-68454890	MS.gene21066:CDS	25.0%
!!	TGATAATTTTCATATTGTCG+TGG	-	chr1.4:68454265-68454284	MS.gene21066:CDS	25.0%
	GAAAATTATCATAGAGCCAT+GGG	+	chr1.4:68454256-68454275	None:intergenic	30.0%
	TCTTCAAACTTGTCCAATTT+GGG	-	chr1.4:68454815-68454834	MS.gene21066:CDS	30.0%
	TGAAAATTATCATAGAGCCA+TGG	+	chr1.4:68454257-68454276	None:intergenic	30.0%
	TTCCTTAGGTGAAAATGTTA+GGG	-	chr1.4:68455192-68455211	MS.gene21066:CDS	30.0%
	TTCTTCAAACTTGTCCAATT+TGG	-	chr1.4:68454814-68454833	MS.gene21066:CDS	30.0%
	TTTCCTTAGGTGAAAATGTT+AGG	-	chr1.4:68455191-68455210	MS.gene21066:CDS	30.0%
!	AATTTTCTTTTGCAGCTGAT+GGG	-	chr1.4:68455155-68455174	MS.gene21066:CDS	30.0%
!	ATTAATGGGGATTTTTCCTT+AGG	-	chr1.4:68455178-68455197	MS.gene21066:CDS	30.0%
!	ATTTGTGATGGAACTGAAAT+AGG	+	chr1.4:68454205-68454224	None:intergenic	30.0%
	AACAGAAATACAAGCAGAAG+TGG	+	chr1.4:68454969-68454988	None:intergenic	35.0%
	AAGTTTGAAGAACTTTCAGC+AGG	+	chr1.4:68454806-68454825	None:intergenic	35.0%
	AATGTTGTACAGCAAACCAA+TGG	-	chr1.4:68454743-68454762	MS.gene21066:CDS	35.0%
	AATTCTGCCTTGTCTAAAAG+TGG	-	chr1.4:68454902-68454921	MS.gene21066:CDS	35.0%
	ATCTGAACCATCTAGATCTT+TGG	-	chr1.4:68454324-68454343	MS.gene21066:CDS	35.0%
	CATAATTCCCATTGTTTGAG+CGG	+	chr1.4:68454523-68454542	None:intergenic	35.0%
	CCATCAAAATTCAAAGCTTG+AGG	+	chr1.4:68453987-68454006	None:intergenic	35.0%
	CTTTGTTGGATGAAACCAAA+GGG	+	chr1.4:68454368-68454387	None:intergenic	35.0%
	GAAAATTCAGTTTCTTCCTG+AGG	+	chr1.4:68455142-68455161	None:intergenic	35.0%
	TAGTTCATACATGCGTTTGA+AGG	-	chr1.4:68454454-68454473	MS.gene21066:CDS	35.0%
	TCCTTAGGTGAAAATGTTAG+GGG	-	chr1.4:68455193-68455212	MS.gene21066:CDS	35.0%
	TCTGAACCATCTAGATCTTT+GGG	-	chr1.4:68454325-68454344	MS.gene21066:CDS	35.0%
	TTGCTGTACAACATTTGGAA+TGG	+	chr1.4:68454738-68454757	None:intergenic	35.0%
	TTTGAACTTGACTCTGATGA+TGG	-	chr1.4:68454632-68454651	MS.gene21066:CDS	35.0%
!	CTTTGGTTTCATCCAACAAA+GGG	-	chr1.4:68454367-68454386	MS.gene21066:CDS	35.0%
!	GAATTTTCTTTTGCAGCTGA+TGG	-	chr1.4:68455154-68455173	MS.gene21066:CDS	35.0%
!	GGAGAAATGAAAGTGTATGA+TGG	-	chr1.4:68454416-68454435	MS.gene21066:CDS	35.0%
!	TGCTGTACAACATTTGGAAT+GGG	+	chr1.4:68454737-68454756	None:intergenic	35.0%
!!	TTGGTTTGCTGTACAACATT+TGG	+	chr1.4:68454743-68454762	None:intergenic	35.0%
!!!	CCTCAAGCTTTGAATTTTGA+TGG	-	chr1.4:68453984-68454003	MS.gene21066:CDS	35.0%
	AAATACAAGCAGAAGTGGAC+AGG	+	chr1.4:68454964-68454983	None:intergenic	40.0%
	AAATACGACCGATCAGACAA+TGG	+	chr1.4:68454079-68454098	None:intergenic	40.0%
	AACATGTTCAGCACCTGTAA+TGG	+	chr1.4:68455029-68455048	None:intergenic	40.0%
	ACTTTCAGCAGGAAACTGAT+AGG	+	chr1.4:68454795-68454814	None:intergenic	40.0%
	AGTGTATGATGGCTGTAACT+CGG	-	chr1.4:68454427-68454446	MS.gene21066:CDS	40.0%
	ATCCTTGACCATTGTCTGAT+CGG	-	chr1.4:68454068-68454087	MS.gene21066:CDS	40.0%
	CAAGCTATTGGTCAGTTTCT+CGG	-	chr1.4:68454395-68454414	MS.gene21066:CDS	40.0%
	CCTTAGGTGAAAATGTTAGG+GGG	-	chr1.4:68455194-68455213	MS.gene21066:CDS	40.0%
	CCTTTGTTGGATGAAACCAA+AGG	+	chr1.4:68454369-68454388	None:intergenic	40.0%
	CTAAGTGAATGTCTCCAAAG+TGG	-	chr1.4:68455112-68455131	MS.gene21066:CDS	40.0%
	CTTAGGTGAAAATGTTAGGG+GGG	-	chr1.4:68455195-68455214	MS.gene21066:CDS	40.0%
	CTTGTACCCAAAGATCTAGA+TGG	+	chr1.4:68454334-68454353	None:intergenic	40.0%
	GATCTTTGGGTACAAGTTCA+CGG	-	chr1.4:68454338-68454357	MS.gene21066:CDS	40.0%
	GATGGAACTGAAATAGGTAG+CGG	+	chr1.4:68454199-68454218	None:intergenic	40.0%
	GGTTCAGATCAATGTTTCGT+GGG	+	chr1.4:68454313-68454332	None:intergenic	40.0%
	TAGGACATACCTTGTCAGTA+TGG	+	chr1.4:68454607-68454626	None:intergenic	40.0%
	TCAAACTTGTCCAATTTGGG+TGG	-	chr1.4:68454818-68454837	MS.gene21066:CDS	40.0%
	TCAGGTCTGAACTGAGAAAA+AGG	-	chr1.4:68455080-68455099	MS.gene21066:CDS	40.0%
	TGCCTTGTCTAAAAGTGGTA+AGG	-	chr1.4:68454907-68454926	MS.gene21066:CDS	40.0%
	TGGTTCAGATCAATGTTTCG+TGG	+	chr1.4:68454314-68454333	None:intergenic	40.0%
	TTAGGTGAAAATGTTAGGGG+GGG	-	chr1.4:68455196-68455215	MS.gene21066:CDS	40.0%
	TTGCAGCTGATGGGATTAAT+GGG	-	chr1.4:68455164-68455183	MS.gene21066:CDS	40.0%
	TTTGCAGCTGATGGGATTAA+TGG	-	chr1.4:68455163-68455182	MS.gene21066:CDS	40.0%
!	CCTTTGGTTTCATCCAACAA+AGG	-	chr1.4:68454366-68454385	MS.gene21066:CDS	40.0%
!	GCTGTACAACATTTGGAATG+GGG	+	chr1.4:68454736-68454755	None:intergenic	40.0%
!	TCTGCTTGTATTTCTGTTGC+TGG	-	chr1.4:68454971-68454990	MS.gene21066:CDS	40.0%
!	TGGCAGCATCTAATTTGTGA+TGG	+	chr1.4:68454217-68454236	None:intergenic	40.0%
!	TTGTCTGATCGGTCGTATTT+TGG	-	chr1.4:68454079-68454098	MS.gene21066:CDS	40.0%
!!	AGCCTTACCACTTTTAGACA+AGG	+	chr1.4:68454912-68454931	None:intergenic	40.0%
!!	TGATGATGGTGTACGTGAAT+GGG	-	chr1.4:68454646-68454665	MS.gene21066:CDS	40.0%
!!!	AACTTTTTTGACGGGTTTCC+AGG	+	chr1.4:68454153-68454172	None:intergenic	40.0%
	AACCTTCAGCAGCTAAGGTA+AGG	+	chr1.4:68454010-68454029	None:intergenic	45.0%
	ACCTGCCATTGCTTCTTTAG+GGG	+	chr1.4:68454500-68454519	None:intergenic	45.0%
	AGCCTGAAACAGAGGATATC+AGG	-	chr1.4:68455062-68455081	MS.gene21066:CDS	45.0%
	AGGGTTATCAGCAAGGTTCA+AGG	+	chr1.4:68454036-68454055	None:intergenic	45.0%
	ATCAGCCCCTAAAGAAGCAA+TGG	-	chr1.4:68454492-68454511	MS.gene21066:CDS	45.0%
	CAAACCAATGGCACTAATGG+AGG	-	chr1.4:68454755-68454774	MS.gene21066:CDS	45.0%
	CAGACAATGGTCAAGGATCT+GGG	+	chr1.4:68454066-68454085	None:intergenic	45.0%
	CAGCAAACCAATGGCACTAA+TGG	-	chr1.4:68454752-68454771	MS.gene21066:CDS	45.0%
	CATACATGCGTTTGAAGGTC+AGG	-	chr1.4:68454459-68454478	MS.gene21066:CDS	45.0%
	GACCTGATATCCTCTGTTTC+AGG	+	chr1.4:68455067-68455086	None:intergenic	45.0%
	GACCTGCCATTGCTTCTTTA+GGG	+	chr1.4:68454501-68454520	None:intergenic	45.0%
	GATATCCTCTGTTTCAGGCT+CGG	+	chr1.4:68455062-68455081	None:intergenic	45.0%
	GGTTTCATCCAACAAAGGGT+GGG	-	chr1.4:68454371-68454390	MS.gene21066:CDS	45.0%
	TAGTGGCTGTAGTCCCAATA+CGG	+	chr1.4:68454697-68454716	None:intergenic	45.0%
	TCACTTCAGGGTTATCAGCA+AGG	+	chr1.4:68454043-68454062	None:intergenic	45.0%
	TCAGACAATGGTCAAGGATC+TGG	+	chr1.4:68454067-68454086	None:intergenic	45.0%
	TCCCAATACGGTGGACATTA+GGG	+	chr1.4:68454685-68454704	None:intergenic	45.0%
	TGCAGCTGATGGGATTAATG+GGG	-	chr1.4:68455165-68455184	MS.gene21066:CDS	45.0%
	TGGTTTCATCCAACAAAGGG+TGG	-	chr1.4:68454370-68454389	MS.gene21066:CDS	45.0%
	TGTTCCTCCATTAGTGCCAT+TGG	+	chr1.4:68454762-68454781	None:intergenic	45.0%
!	ATGATGGTGTACGTGAATGG+GGG	-	chr1.4:68454648-68454667	MS.gene21066:CDS	45.0%
!	CATGATGAACAGCACTGAGT+CGG	+	chr1.4:68454844-68454863	None:intergenic	45.0%
!	CCCCCTAACATTTTCACCTA+AGG	+	chr1.4:68455197-68455216	None:intergenic	45.0%
!	GATGATGGTGTACGTGAATG+GGG	-	chr1.4:68454647-68454666	MS.gene21066:CDS	45.0%
!	TGGACAGGAAAGCATGGTTA+AGG	+	chr1.4:68454949-68454968	None:intergenic	45.0%
!!	CTGATGATGGTGTACGTGAA+TGG	-	chr1.4:68454645-68454664	MS.gene21066:CDS	45.0%
!!	TCAGCACCTGTAATGGATAG+AGG	+	chr1.4:68455022-68455041	None:intergenic	45.0%
	ATGTTTCGTGGGACAACACC+TGG	+	chr1.4:68454302-68454321	None:intergenic	50.0%
	CAAAGGGTGGGACAAGCTAT+TGG	-	chr1.4:68454383-68454402	MS.gene21066:CDS	50.0%
	CAAGGATCTGGGTCACTTCA+GGG	+	chr1.4:68454055-68454074	None:intergenic	50.0%
	CAGAAGTGGACAGGAAAGCA+TGG	+	chr1.4:68454955-68454974	None:intergenic	50.0%
	GACCGATCAGACAATGGTCA+AGG	+	chr1.4:68454073-68454092	None:intergenic	50.0%
	GCCCTAATGTCCACCGTATT+GGG	-	chr1.4:68454681-68454700	MS.gene21066:CDS	50.0%
	GGACCTGCCATTGCTTCTTT+AGG	+	chr1.4:68454502-68454521	None:intergenic	50.0%
	GGTCAAACCTTCAGCAGCTA+AGG	+	chr1.4:68454015-68454034	None:intergenic	50.0%
	GTCCCAATACGGTGGACATT+AGG	+	chr1.4:68454686-68454705	None:intergenic	50.0%
	GTCGTGGAAAGAATCAGTCC+AGG	-	chr1.4:68454281-68454300	MS.gene21066:CDS	50.0%
	GTCTTGAAGCCACCTGTTAG+TGG	+	chr1.4:68454714-68454733	None:intergenic	50.0%
	TCAAGGATCTGGGTCACTTC+AGG	+	chr1.4:68454056-68454075	None:intergenic	50.0%
	TCTGCTTGGCCATACTGACA+AGG	-	chr1.4:68454595-68454614	MS.gene21066:CDS	50.0%
	TGCCCTAATGTCCACCGTAT+TGG	-	chr1.4:68454680-68454699	MS.gene21066:CDS	50.0%
	TTGGGACTACAGCCACTAAC+AGG	-	chr1.4:68454699-68454718	MS.gene21066:CDS	50.0%
!	GGGGTCTTTTGTTACCGTTG+CGG	-	chr1.4:68454572-68454591	MS.gene21066:CDS	50.0%
!	TAGCTTGTCCCACCCTTTGT+TGG	+	chr1.4:68454382-68454401	None:intergenic	50.0%
!	TGATGGTGTACGTGAATGGG+GGG	-	chr1.4:68454649-68454668	MS.gene21066:CDS	50.0%
!!	TTTGACGGGTTTCCAGGCTT+TGG	+	chr1.4:68454147-68454166	None:intergenic	50.0%
	ACTGAGTCGGCCACCCAAAT+TGG	+	chr1.4:68454831-68454850	None:intergenic	55.0%
	GCAGGAAACTGATAGGCAGC+AGG	+	chr1.4:68454788-68454807	None:intergenic	55.0%
	GCCCCTAAAGAAGCAATGGC+AGG	-	chr1.4:68454496-68454515	MS.gene21066:CDS	55.0%
	GCGGCACCTCTATCCATTAC+AGG	-	chr1.4:68455013-68455032	MS.gene21066:CDS	55.0%
	GCTGCCAAAGTCCTCGATGA+TGG	-	chr1.4:68454230-68454249	MS.gene21066:CDS	55.0%
	GGACTACAGCCACTAACAGG+TGG	-	chr1.4:68454702-68454721	MS.gene21066:CDS	55.0%
	GGCAGGTCCGCTCAAACAAT+GGG	-	chr1.4:68454513-68454532	MS.gene21066:CDS	55.0%
	GGCCTTACCTTAGCTGCTGA+AGG	-	chr1.4:68454005-68454024	MS.gene21066:CDS	55.0%
	TATGGCCAAGCAGACCGCAA+CGG	+	chr1.4:68454589-68454608	None:intergenic	55.0%
	TGGCAGGTCCGCTCAAACAA+TGG	-	chr1.4:68454512-68454531	MS.gene21066:CDS	55.0%
	TGGCTGTAGTCCCAATACGG+TGG	+	chr1.4:68454694-68454713	None:intergenic	55.0%
	TGGGCCATCATCGAGGACTT+TGG	+	chr1.4:68454237-68454256	None:intergenic	55.0%
	TGTTACCGTTGCGGTCTGCT+TGG	-	chr1.4:68454581-68454600	MS.gene21066:CDS	55.0%
!	AAGTCCTCGATGATGGCCCA+TGG	-	chr1.4:68454237-68454256	MS.gene21066:CDS	55.0%
!	CAAGTTCACGGACTGCCCTT+TGG	-	chr1.4:68454350-68454369	MS.gene21066:CDS	55.0%
!	CCTCTGTTTCAGGCTCGGTA+GGG	+	chr1.4:68455057-68455076	None:intergenic	55.0%
!	CTCTGTTTCAGGCTCGGTAG+GGG	+	chr1.4:68455056-68455075	None:intergenic	55.0%
!	TCCTCTGTTTCAGGCTCGGT+AGG	+	chr1.4:68455058-68455077	None:intergenic	55.0%
!!	GTAATGGATAGAGGTGCCGC+AGG	+	chr1.4:68455013-68455032	None:intergenic	55.0%
!!	TTGCTGGTTCACTCCCCCAA+AGG	-	chr1.4:68454987-68455006	MS.gene21066:CDS	55.0%
	AGAGCCATGGGCCATCATCG+AGG	+	chr1.4:68454244-68454263	None:intergenic	60.0%
	CCCTACCGAGCCTGAAACAG+AGG	-	chr1.4:68455054-68455073	MS.gene21066:CDS	60.0%
	GTGAGCGCATGTCCAAAGCC+TGG	-	chr1.4:68454132-68454151	MS.gene21066:CDS	60.0%
	TGGTGTACGTGAATGGGGGG+TGG	-	chr1.4:68454652-68454671	MS.gene21066:CDS	60.0%
	TTCACTCCCCCAAAGGCCTG+CGG	-	chr1.4:68454994-68455013	MS.gene21066:CDS	60.0%
!!	ATAGAGGTGCCGCAGGCCTT+TGG	+	chr1.4:68455006-68455025	None:intergenic	60.0%
!!	TAGAGGTGCCGCAGGCCTTT+GGG	+	chr1.4:68455005-68455024	None:intergenic	60.0%
	CTTCCTGAGGCGCGCCACTT+TGG	+	chr1.4:68455129-68455148	None:intergenic	65.0%
	TCTCCAAAGTGGCGCGCCTC+AGG	-	chr1.4:68455123-68455142	MS.gene21066:CDS	65.0%
!!	AGAGGTGCCGCAGGCCTTTG+GGG	+	chr1.4:68455004-68455023	None:intergenic	65.0%
!!	GAGGTGCCGCAGGCCTTTGG+GGG	+	chr1.4:68455003-68455022	None:intergenic	70.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	68453978	68455231	68453978	ID=MS.gene21066
chr1.4	mRNA	68453978	68455231	68453978	ID=MS.gene21066.t1;Parent=MS.gene21066
chr1.4	exon	68453978	68455231	68453978	ID=MS.gene21066.t1.exon1;Parent=MS.gene21066.t1
chr1.4	CDS	68453978	68455231	68453978	ID=cds.MS.gene21066.t1;Parent=MS.gene21066.t1

Gene Sequence

>MS.gene21066

ATGGATCCTCAAGCTTTGAATTTTGATGGCCTTACCTTAGCTGCTGAAGGTTTGACCTTGAACCTTGCTGATAACCCTGAAGTGACCCAGATCCTTGACCATTGTCTGATCGGTCGTATTTTGGCTGATCGACAAATCAAATTTGCATATTTTAGTGAGCGCATGTCCAAAGCCTGGAAACCCGTCAAAAAAGTTACAATTTTGCAATATGGTGATGACCGCTACCTATTTCAGTTCCATCACAAATTAGATGCTGCCAAAGTCCTCGATGATGGCCCATGGCTCTATGATAATTTTCATATTGTCGTGGAAAGAATCAGTCCAGGTGTTGTCCCACGAAACATTGATCTGAACCATCTAGATCTTTGGGTACAAGTTCACGGACTGCCCTTTGGTTTCATCCAACAAAGGGTGGGACAAGCTATTGGTCAGTTTCTCGGAGAAATGAAAGTGTATGATGGCTGTAACTCGGTACATAGTTCATACATGCGTTTGAAGGTCAGGATCAATGTGAATCAGCCCCTAAAGAAGCAATGGCAGGTCCGCTCAAACAATGGGAATTATGTGACTATCCAATTTAAATATGAAAAGTTGGGGGTCTTTTGTTACCGTTGCGGTCTGCTTGGCCATACTGACAAGGTATGTCCTAATTTATTTGAACTTGACTCTGATGATGGTGTACGTGAATGGGGGGTGGAGCTGTGCCCTAATGTCCACCGTATTGGGACTACAGCCACTAACAGGTGGCTTCAAGACCCCATTCCAAATGTTGTACAGCAAACCAATGGCACTAATGGAGGAACACAACCTGCTGCCTATCAGTTTCCTGCTGAAAGTTCTTCAAACTTGTCCAATTTGGGTGGCCGACTCAGTGCTGTTCATCATGAGATAAATGCTATTAAACAAGGAATTATGGTGGTTCAAAATTCTGCCTTGTCTAAAAGTGGTAAGGCTACTGCTGCTGTTCACCTTAACCATGCTTTCCTGTCCACTTCTGCTTGTATTTCTGTTGCTGGTTCACTCCCCCAAAGGCCTGCGGCACCTCTATCCATTACAGGTGCTGAACATGTTTCTGCCCCTACCGAGCCTGAAACAGAGGATATCAGGTCTGAACTGAGAAAAAGGAAGCGTATGCTAAGTGAATGTCTCCAAAGTGGCGCGCCTCAGGAAGAAACTGAATTTTCTTTTGCAGCTGATGGGATTAATGGGGATTTTTCCTTAGGTGAAAATGTTAGGGGGGGAATGATACTGTAA

Protein sequence

>MS.gene21066.t1

MDPQALNFDGLTLAAEGLTLNLADNPEVTQILDHCLIGRILADRQIKFAYFSERMSKAWKPVKKVTILQYGDDRYLFQFHHKLDAAKVLDDGPWLYDNFHIVVERISPGVVPRNIDLNHLDLWVQVHGLPFGFIQQRVGQAIGQFLGEMKVYDGCNSVHSSYMRLKVRINVNQPLKKQWQVRSNNGNYVTIQFKYEKLGVFCYRCGLLGHTDKVCPNLFELDSDDGVREWGVELCPNVHRIGTTATNRWLQDPIPNVVQQTNGTNGGTQPAAYQFPAESSSNLSNLGGRLSAVHHEINAIKQGIMVVQNSALSKSGKATAAVHLNHAFLSTSACISVAGSLPQRPAAPLSITGAEHVSAPTEPETEDIRSELRKRKRMLSECLQSGAPQEETEFSFAADGINGDFSLGENVRGGMIL