AlfalfaGEDB | MS.gene60059

M. sativa cultivar XinJiangDaYe / MS.gene60059

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60059.t1	KEH25633.1	82.5	194	34	0	2	195	102	295	1.40E-85	326.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60059.t1	A0A072U949	82.5	194	34	0	2	195	102	295	1.0e-85	326.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene60059	MS.gene71637	PPI
MS.gene60059	MS.gene55209	PPI
MS.gene60059	MS.gene96174	PPI
MS.gene60059	MS.gene28718	PPI
MS.gene60059	MS.gene022929	PPI
MS.gene60059	MS.gene85295	PPI
MS.gene94654	MS.gene60059	PPI
MS.gene60059	MS.gene068629	PPI
MS.gene60059	MS.gene009781	PPI
MS.gene60059	MS.gene047954	PPI
MS.gene83772	MS.gene60059	PPI
MS.gene60059	MS.gene048342	PPI
MS.gene60059	MS.gene069624	PPI
MS.gene96541	MS.gene60059	PPI
MS.gene60059	MS.gene83979	PPI
MS.gene60059	MS.gene21066	PPI
MS.gene60059	MS.gene013890	PPI
MS.gene63762	MS.gene60059	PPI
MS.gene60059	MS.gene073582	PPI
MS.gene30208	MS.gene60059	PPI
MS.gene025873	MS.gene60059	PPI
MS.gene60059	MS.gene048072	PPI
MS.gene60059	MS.gene028714	PPI
MS.gene60059	MS.gene042027	PPI
MS.gene60059	MS.gene048706	PPI
MS.gene60059	MS.gene69586	PPI
MS.gene60059	MS.gene45083	PPI
MS.gene05358	MS.gene60059	PPI
MS.gene60059	MS.gene049561	PPI
MS.gene60059	MS.gene008658	PPI
MS.gene60059	MS.gene47792	PPI
MS.gene030090	MS.gene60059	PPI
MS.gene95561	MS.gene60059	PPI
MS.gene60059	MS.gene80362	PPI
MS.gene60059	MS.gene49057	PPI
MS.gene018196	MS.gene60059	PPI
MS.gene60059	MS.gene03936	PPI
MS.gene60059	MS.gene43393	PPI
MS.gene065627	MS.gene60059	PPI
MS.gene60059	MS.gene048088	PPI
MS.gene57019	MS.gene60059	PPI
MS.gene60059	MS.gene08708	PPI
MS.gene60059	MS.gene86275	PPI
MS.gene60059	MS.gene034643	PPI
MS.gene038098	MS.gene60059	PPI
MS.gene60059	MS.gene038098	PPI
MS.gene28545	MS.gene60059	PPI
MS.gene60059	MS.gene012889	PPI
MS.gene60059	MS.gene038757	PPI
MS.gene60059	MS.gene000506	PPI
MS.gene008275	MS.gene60059	PPI
MS.gene60059	MS.gene39956	PPI
MS.gene064966	MS.gene60059	PPI
MS.gene60059	MS.gene028716	PPI
MS.gene60059	MS.gene74639	PPI
MS.gene60059	MS.gene071133	PPI
MS.gene60059	MS.gene60266	PPI
MS.gene60059	MS.gene98467	PPI
MS.gene60059	MS.gene51804	PPI
MS.gene60059	MS.gene013644	PPI
MS.gene60059	MS.gene54051	PPI
MS.gene60059	MS.gene22111	PPI
MS.gene60059	MS.gene049610	PPI
MS.gene60059	MS.gene34197	PPI
MS.gene60059	MS.gene045875	PPI
MS.gene60059	MS.gene010890	PPI
MS.gene60059	MS.gene84790	PPI
MS.gene036423	MS.gene60059	PPI
MS.gene60059	MS.gene055683	PPI
MS.gene60059	MS.gene061579	PPI
MS.gene60059	MS.gene41979	PPI
MS.gene60059	MS.gene04970	PPI
MS.gene72520	MS.gene60059	PPI
MS.gene66651	MS.gene60059	PPI
MS.gene96109	MS.gene60059	PPI
MS.gene60059	MS.gene26038	PPI
MS.gene60059	MS.gene060788	PPI
MS.gene60059	MS.gene43924	PPI
MS.gene051449	MS.gene60059	PPI
MS.gene60059	MS.gene41643	PPI
MS.gene60059	MS.gene77990	PPI
MS.gene60059	MS.gene062511	PPI
MS.gene60059	MS.gene008861	PPI
MS.gene60059	MS.gene027994	PPI
MS.gene60059	MS.gene26433	PPI
MS.gene60059	MS.gene007022	PPI
MS.gene60059	MS.gene045614	PPI
MS.gene60059	MS.gene064966	PPI
MS.gene60059	MS.gene69584	PPI
MS.gene60059	MS.gene52349	PPI
MS.gene30136	MS.gene60059	PPI
MS.gene60059	MS.gene021647	PPI
MS.gene60059	MS.gene76420	PPI
MS.gene60059	MS.gene46353	PPI
MS.gene60059	MS.gene65471	PPI
MS.gene60059	MS.gene071784	PPI
MS.gene83979	MS.gene60059	PPI
MS.gene016102	MS.gene60059	PPI
MS.gene46941	MS.gene60059	PPI
MS.gene60059	MS.gene060386	PPI
MS.gene60059	MS.gene61651	PPI
MS.gene60059	MS.gene29406	PPI
MS.gene47792	MS.gene60059	PPI
MS.gene46948	MS.gene60059	PPI
MS.gene60059	MS.gene63762	PPI
MS.gene60059	MS.gene016719	PPI
MS.gene012019	MS.gene60059	PPI
MS.gene60059	MS.gene88393	PPI
MS.gene60059	MS.gene054508	PPI
MS.gene60059	MS.gene053373	PPI
MS.gene93396	MS.gene60059	PPI
MS.gene60059	MS.gene041264	PPI
MS.gene60059	MS.gene35175	PPI
MS.gene60059	MS.gene63150	PPI
MS.gene05231	MS.gene60059	PPI
MS.gene60059	MS.gene44083	PPI
MS.gene60059	MS.gene000629	PPI
MS.gene010656	MS.gene60059	PPI
MS.gene60059	MS.gene072852	PPI
MS.gene000629	MS.gene60059	PPI
MS.gene60059	MS.gene65474	PPI
MS.gene60059	MS.gene025401	PPI
MS.gene60059	MS.gene57044	PPI
MS.gene60059	MS.gene015093	PPI
MS.gene60059	MS.gene04134	PPI
MS.gene60059	MS.gene06164	PPI
MS.gene05319	MS.gene60059	PPI
MS.gene60059	MS.gene50392	PPI
MS.gene60059	MS.gene22730	PPI
MS.gene56991	MS.gene60059	PPI
MS.gene050679	MS.gene60059	PPI
MS.gene60059	MS.gene032299	PPI
MS.gene60059	MS.gene017586	PPI
MS.gene60059	MS.gene037634	PPI
MS.gene20246	MS.gene60059	PPI
MS.gene60059	MS.gene057828	PPI
MS.gene60059	MS.gene071320	PPI
MS.gene055223	MS.gene60059	PPI
MS.gene60059	MS.gene08606	PPI
MS.gene60059	MS.gene012019	PPI
MS.gene60059	MS.gene000640	PPI
MS.gene60059	MS.gene09517	PPI
MS.gene60059	MS.gene061850	PPI
MS.gene60059	MS.gene98756	PPI
MS.gene60059	MS.gene87845	PPI
MS.gene061850	MS.gene60059	PPI
MS.gene60059	MS.gene85308	PPI
MS.gene60059	MS.gene64130	PPI
MS.gene60059	MS.gene62249	PPI
MS.gene60059	MS.gene024522	PPI
MS.gene60059	MS.gene046089	PPI
MS.gene60059	MS.gene010290	PPI
MS.gene60059	MS.gene053747	PPI
MS.gene98616	MS.gene60059	PPI
MS.gene60059	MS.gene92517	PPI
MS.gene60059	MS.gene021555	PPI
MS.gene60059	MS.gene36069	PPI
MS.gene60059	MS.gene71675	PPI
MS.gene60059	MS.gene044729	PPI
MS.gene60059	MS.gene05319	PPI
MS.gene69490	MS.gene60059	PPI
MS.gene60059	MS.gene60390	PPI
MS.gene60059	MS.gene039692	PPI
MS.gene015248	MS.gene60059	PPI
MS.gene60059	MS.gene032311	PPI
MS.gene60059	MS.gene55682	PPI
MS.gene60059	MS.gene002590	PPI
MS.gene60059	MS.gene81712	PPI
MS.gene60059	MS.gene008927	PPI
MS.gene013193	MS.gene60059	PPI
MS.gene08106	MS.gene60059	PPI
MS.gene60059	MS.gene98616	PPI
MS.gene60059	MS.gene23521	PPI
MS.gene60059	MS.gene37955	PPI
MS.gene60059	MS.gene005120	PPI
MS.gene60059	MS.gene65546	PPI
MS.gene60059	MS.gene024297	PPI
MS.gene60059	MS.gene059269	PPI
MS.gene60059	MS.gene057934	PPI
MS.gene60059	MS.gene024841	PPI
MS.gene60059	MS.gene069786	PPI
MS.gene60059	MS.gene025158	PPI
MS.gene60059	MS.gene40828	PPI
MS.gene60059	MS.gene87529	PPI
MS.gene60059	MS.gene51799	PPI
MS.gene062067	MS.gene60059	PPI
MS.gene60059	MS.gene98471	PPI
MS.gene60059	MS.gene062067	PPI
MS.gene60059	MS.gene97410	PPI
MS.gene60059	MS.gene86849	PPI
MS.gene60059	MS.gene013676	PPI
MS.gene57044	MS.gene60059	PPI
MS.gene60059	MS.gene40469	PPI
MS.gene038676	MS.gene60059	PPI
MS.gene60059	MS.gene09023	PPI
MS.gene60059	MS.gene66651	PPI
MS.gene60059	MS.gene025987	PPI
MS.gene60059	MS.gene95960	PPI
MS.gene60059	MS.gene043140	PPI
MS.gene042651	MS.gene60059	PPI
MS.gene60059	MS.gene61452	PPI
MS.gene60059	MS.gene05231	PPI
MS.gene60059	MS.gene03149	PPI
MS.gene60059	MS.gene29321	PPI
MS.gene60059	MS.gene018745	PPI
MS.gene60059	MS.gene037049	PPI
MS.gene60059	MS.gene24278	PPI
MS.gene60059	MS.gene000379	PPI
MS.gene60059	MS.gene34618	PPI
MS.gene60059	MS.gene59460	PPI
MS.gene60059	MS.gene060773	PPI
MS.gene60059	MS.gene66650	PPI
MS.gene60059	MS.gene009121	PPI
MS.gene60059	MS.gene08713	PPI
MS.gene60059	MS.gene09589	PPI
MS.gene60059	MS.gene70932	PPI
MS.gene60059	MS.gene024405	PPI
MS.gene60059	MS.gene021965	PPI
MS.gene42903	MS.gene60059	PPI
MS.gene60059	MS.gene24070	PPI
MS.gene60059	MS.gene059736	PPI
MS.gene60059	MS.gene58419	PPI
MS.gene60059	MS.gene26844	PPI
MS.gene60059	MS.gene64178	PPI
MS.gene048747	MS.gene60059	PPI
MS.gene042559	MS.gene60059	PPI
MS.gene60059	MS.gene074257	PPI
MS.gene22200	MS.gene60059	PPI
MS.gene60059	MS.gene04749	PPI
MS.gene60059	MS.gene073573	PPI
MS.gene60059	MS.gene44144	PPI
MS.gene60059	MS.gene05576	PPI
MS.gene60059	MS.gene060718	PPI
MS.gene60059	MS.gene63743	PPI
MS.gene60059	MS.gene48477	PPI
MS.gene60059	MS.gene055223	PPI
MS.gene031967	MS.gene60059	PPI
MS.gene60059	MS.gene027169	PPI
MS.gene34812	MS.gene60059	PPI
MS.gene60059	MS.gene004417	PPI
MS.gene60059	MS.gene019177	PPI
MS.gene60059	MS.gene031967	PPI
MS.gene60059	MS.gene05436	PPI
MS.gene063214	MS.gene60059	PPI
MS.gene97410	MS.gene60059	PPI
MS.gene32415	MS.gene60059	PPI
MS.gene60059	MS.gene038623	PPI
MS.gene035626	MS.gene60059	PPI
MS.gene067340	MS.gene60059	PPI
MS.gene60059	MS.gene055560	PPI
MS.gene60059	MS.gene009213	PPI
MS.gene60059	MS.gene98127	PPI
MS.gene60059	MS.gene35202	PPI
MS.gene60059	MS.gene003264	PPI
MS.gene60059	MS.gene054652	PPI
MS.gene60059	MS.gene000634	PPI
MS.gene60059	MS.gene37537	PPI
MS.gene69495	MS.gene60059	PPI
MS.gene038623	MS.gene60059	PPI
MS.gene60059	MS.gene42010	PPI
MS.gene05357	MS.gene60059	PPI
MS.gene60059	MS.gene049512	PPI
MS.gene032311	MS.gene60059	PPI
MS.gene60059	MS.gene27329	PPI
MS.gene60059	MS.gene41393	PPI
MS.gene60059	MS.gene89815	PPI
MS.gene60059	MS.gene010656	PPI
MS.gene60059	MS.gene016632	PPI
MS.gene60059	MS.gene018474	PPI
MS.gene60059	MS.gene86868	PPI
MS.gene60059	MS.gene058889	PPI
MS.gene60059	MS.gene96163	PPI
MS.gene60059	MS.gene65623	PPI
MS.gene021968	MS.gene60059	PPI
MS.gene60059	MS.gene012748	PPI
MS.gene60059	MS.gene02896	PPI
MS.gene09589	MS.gene60059	PPI
MS.gene60059	MS.gene71588	PPI
MS.gene43393	MS.gene60059	PPI
MS.gene60059	MS.gene34463	PPI
MS.gene60059	MS.gene042614	PPI
MS.gene60059	MS.gene026680	PPI
MS.gene60059	MS.gene61499	PPI
MS.gene60059	MS.gene051449	PPI
MS.gene048072	MS.gene60059	PPI
MS.gene60059	MS.gene019165	PPI
MS.gene04749	MS.gene60059	PPI
MS.gene60059	MS.gene81194	PPI
MS.gene05436	MS.gene60059	PPI
MS.gene60059	MS.gene038394	PPI
MS.gene60059	MS.gene019001	PPI
MS.gene60059	MS.gene53904	PPI
MS.gene60059	MS.gene73261	PPI
MS.gene055683	MS.gene60059	PPI
MS.gene60059	MS.gene85538	PPI
MS.gene60059	MS.gene025675	PPI
MS.gene60059	MS.gene055546	PPI
MS.gene60059	MS.gene83772	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60059.t1	MTR_6g033215	82.474	194	34	0	2	195	102	295	4.60e-115	333
MS.gene60059.t1	MTR_6g053790	49.624	133	42	3	131	262	35	143	2.84e-25	102
MS.gene60059.t1	MTR_6g053790	82.857	35	6	0	1	35	1	35	2.69e-11	63.5
MS.gene60059.t1	MTR_4g075740	47.287	129	48	4	2	128	22	132	7.48e-25	97.8
MS.gene60059.t1	MTR_8g468010	62.500	64	24	0	110	173	1	64	1.04e-24	96.7
MS.gene60059.t1	MTR_6g053230	27.027	185	96	2	1	182	22	170	6.00e-21	92.4
MS.gene60059.t1	MTR_0010s0370	29.870	154	108	0	19	172	24	177	1.16e-20	87.4
MS.gene60059.t1	MTR_0010s0350	29.870	154	108	0	19	172	24	177	1.16e-20	87.4
MS.gene60059.t1	MTR_1g032270	50.000	76	38	0	93	168	1	76	1.48e-19	83.2
MS.gene60059.t1	MTR_3g024240	32.836	134	87	2	64	194	41	174	2.52e-14	70.1
MS.gene60059.t1	MTR_3g053920	23.602	161	116	2	22	175	29	189	1.19e-12	67.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene60059.t1	AT2G01050	22.951	183	135	2	28	210	80	256	3.37e-12	66.6

Find 81 sgRNAs with CRISPR-Local

Find 93 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CCAGTTGCTGTTTGGATTCA+TGG	0.211086	6.1:-35173066	MS.gene60059:CDS
CCATGAATTCTACGGAAATA+TGG	0.215824	6.1:-35173100	MS.gene60059:CDS
ATTCTGAAGAAAGTCGTAAT+TGG	0.223297	6.1:-35173143	MS.gene60059:CDS
TTCGATGAGGAAAACAATTA+TGG	0.238821	6.1:-35172993	MS.gene60059:CDS
CATGGTCACCATTAATGTTT+AGG	0.261208	6.1:+35173417	None:intergenic
CAGTTTCACCAGTTGCTGTT+TGG	0.261910	6.1:-35173074	MS.gene60059:CDS
TGCTTTGTGTGTGGTGTTAT+AGG	0.291481	6.1:-35172837	MS.gene60059:CDS
ATGGAGAACCTAAACATTAA+TGG	0.292604	6.1:-35173425	MS.gene60059:CDS
GTTGTTAAGTATGAGAAATT+AGG	0.295718	6.1:-35172867	MS.gene60059:CDS
TTCTGAAGAAAGTCGTAATT+GGG	0.331920	6.1:-35173142	MS.gene60059:CDS
CCATATTTCCGTAGAATTCA+TGG	0.332587	6.1:+35173100	None:intergenic
TTGCTAGATTTATGTCTTGT+TGG	0.355887	6.1:-35173356	MS.gene60059:CDS
CCATAGGGATTAGAGGCATT+TGG	0.357060	6.1:+35173258	None:intergenic
TTATCTTCCATAGGGATTAG+AGG	0.365087	6.1:+35173251	None:intergenic
ATTAGAGGCATTTGGTACTT+TGG	0.397524	6.1:+35173266	None:intergenic
TGCAGTGATAAATTTGAATA+CGG	0.400145	6.1:-35172798	MS.gene60059:CDS
AGTCATTCAATGGCAATTGA+TGG	0.403989	6.1:-35172616	MS.gene60059:CDS
AGGCAAGGCTTGGTCTTATG+TGG	0.417288	6.1:-35173292	MS.gene60059:CDS
CCATGAATCCAAACAGCAAC+TGG	0.422078	6.1:+35173066	None:intergenic
GACAAGGTTCGTGCTCTCAT+TGG	0.432800	6.1:-35173041	MS.gene60059:CDS
CATGAATTCTACGGAAATAT+GGG	0.438006	6.1:-35173099	MS.gene60059:CDS
TATGGAAGATAACAAATTCA+TGG	0.447311	6.1:-35173240	MS.gene60059:CDS
CCGAGTGGAAATTGCGGTGG+AGG	0.447923	6.1:-35172946	MS.gene60059:CDS
CATATTGTTGTCGAGTAACC+AGG	0.464718	6.1:+35173169	None:intergenic
TCGATGAGGAAAACAATTAT+GGG	0.470543	6.1:-35172992	MS.gene60059:CDS
GCCACGCTTGAGGCAAGGCT+TGG	0.473876	6.1:-35173302	MS.gene60059:CDS
ATGAATTTGTTATCTTCCAT+AGG	0.475218	6.1:+35173242	None:intergenic
ACAATTATGGGCCGTGGAGG+AGG	0.475628	6.1:-35172980	MS.gene60059:CDS
CCAAATGCCTCTAATCCCTA+TGG	0.475732	6.1:-35173258	MS.gene60059:CDS
GTAACCAGGGGCTACGTTCA+AGG	0.479645	6.1:+35173183	None:intergenic
GATCCTCCTTTAAGAAAAGC+TGG	0.490096	6.1:+35173216	None:intergenic
TGAATTTGTTATCTTCCATA+GGG	0.494634	6.1:+35173243	None:intergenic
CAATCAAAATAGTCATTCAA+TGG	0.494701	6.1:-35172626	MS.gene60059:CDS
GTCACAAAGAGGCAAGCAAA+TGG	0.495466	6.1:-35172711	MS.gene60059:CDS
CTCTTTGTGACCACCACCAA+CGG	0.500901	6.1:+35172723	None:intergenic
GCAAATGGATCGTTGGTGGC+CGG	0.509609	6.1:-35172696	MS.gene60059:CDS
AGGAAAACAATTATGGGCCG+TGG	0.524803	6.1:-35172986	MS.gene60059:CDS
GGCAAGGCTTGGTCTTATGT+GGG	0.527395	6.1:-35173291	MS.gene60059:CDS
GGCCGTGGAGGAGGTTCATG+AGG	0.528202	6.1:-35172971	MS.gene60059:CDS
GCCTTTGTGATGCAGCGCCA+TGG	0.531825	6.1:+35173399	None:intergenic
AATTCTGCCTCAGTGAGGTT+CGG	0.541251	6.1:+35173377	None:intergenic
GGCAAATTCTGCTTTGTGTG+TGG	0.545000	6.1:-35172846	MS.gene60059:CDS
TCTGGTGGCAGGAAGGAAGA+AGG	0.546011	6.1:-35172664	MS.gene60059:CDS
CCTCCACCGCAATTTCCACT+CGG	0.549271	6.1:+35172946	None:intergenic
GACGATGTCCATGAATTCTA+CGG	0.555231	6.1:-35173108	MS.gene60059:CDS
ACTTAGCCACGCTTGAGGCA+AGG	0.558441	6.1:-35173307	MS.gene60059:CDS
GAGAATAATTCCGTTGGTGG+TGG	0.562906	6.1:-35172733	MS.gene60059:CDS
ATAACTAGATCTTGTTGTAA+CGG	0.566636	6.1:+35172921	None:intergenic
ACAAGATCTAGTTATCGAGC+AGG	0.568564	6.1:-35172913	MS.gene60059:CDS
CTAGTTATCGAGCAGGAGGA+AGG	0.569940	6.1:-35172906	MS.gene60059:CDS
GCTGAGAATAATTCCGTTGG+TGG	0.574610	6.1:-35172736	MS.gene60059:CDS
AAATCCTTGAACGTAGCCCC+TGG	0.578482	6.1:-35173187	MS.gene60059:CDS
AATTGATGGTTGAAACATAA+CGG	0.578868	6.1:-35172602	None:intergenic
GCAAGCAAATGGATCGTTGG+TGG	0.582591	6.1:-35172700	MS.gene60059:CDS
GAGGCAAGCAAATGGATCGT+TGG	0.582710	6.1:-35172703	MS.gene60059:CDS
CATGCTTGTTAACCGCCACT+CGG	0.587026	6.1:+35173333	None:intergenic
ATGTCTTGTTGGCCGAGTGG+CGG	0.587119	6.1:-35173345	MS.gene60059:CDS
CTGGTGGCAGGAAGGAAGAA+GGG	0.592314	6.1:-35172663	MS.gene60059:CDS
GTTTGGATTCATGGAAGACA+AGG	0.603393	6.1:-35173057	MS.gene60059:CDS
AGATCTAGTTATCGAGCAGG+AGG	0.605600	6.1:-35172910	MS.gene60059:CDS
CTAAACATTAATGGTGACCA+TGG	0.611623	6.1:-35173416	MS.gene60059:CDS
TCTGAAGAAAGTCGTAATTG+GGG	0.615180	6.1:-35173141	MS.gene60059:CDS
ACCCTCATGAACCTCCTCCA+CGG	0.615762	6.1:+35172969	None:intergenic
GCCGTGGAGGAGGTTCATGA+GGG	0.618700	6.1:-35172970	MS.gene60059:CDS
TGTTCACTTAGCCACGCTTG+AGG	0.629809	6.1:-35173312	MS.gene60059:CDS
CGTTGGTGGTGGTCACAAAG+AGG	0.630275	6.1:-35172722	MS.gene60059:CDS
ACCAAGCCTTGCCTCAAGCG+TGG	0.631098	6.1:+35173301	None:intergenic
ATATTGTTGTCGAGTAACCA+GGG	0.634169	6.1:+35173170	None:intergenic
GGTCCGAGTGGAAATTGCGG+TGG	0.634903	6.1:-35172949	MS.gene60059:CDS
TTTATGTCTTGTTGGCCGAG+TGG	0.637827	6.1:-35173348	MS.gene60059:CDS
ACCATGGCGCTGCATCACAA+AGG	0.646591	6.1:-35173400	MS.gene60059:CDS
TGTGCTGAGAATAATTCCGT+TGG	0.648868	6.1:-35172739	MS.gene60059:CDS
AAAACAATTATGGGCCGTGG+AGG	0.656575	6.1:-35172983	MS.gene60059:CDS
GAGGGTCCGAGTGGAAATTG+CGG	0.659414	6.1:-35172952	MS.gene60059:CDS
GTGTGGTGTTATAGGTCACT+CGG	0.663810	6.1:-35172829	MS.gene60059:CDS
GAGGTTCATGAGGGTCCGAG+TGG	0.668368	6.1:-35172961	MS.gene60059:CDS
AAAGATGATCAAGTTCGATG+AGG	0.683004	6.1:-35173006	MS.gene60059:CDS
CGGAATTATTCTCAGCACAG+AGG	0.694258	6.1:+35172743	None:intergenic
TATTGTTGTCGAGTAACCAG+GGG	0.721242	6.1:+35173171	None:intergenic
CTAGCAATTCTGCCTCAGTG+AGG	0.742691	6.1:+35173372	None:intergenic
ACAAAGGCCGAACCTCACTG+AGG	0.756650	6.1:-35173384	MS.gene60059:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTATTTTGATTGTCATTAA+TGG	+	chr6.1:35173397-35173416	None:intergenic	20.0%
!	CAATCAAAATAGTCATTCAA+TGG	-	chr6.1:35173404-35173423	MS.gene60059:CDS	25.0%
!	GTTGTTAAGTATGAGAAATT+AGG	-	chr6.1:35173163-35173182	MS.gene60059:CDS	25.0%
!	TATGGAAGATAACAAATTCA+TGG	-	chr6.1:35172790-35172809	MS.gene60059:CDS	25.0%
!	TGCAGTGATAAATTTGAATA+CGG	-	chr6.1:35173232-35173251	MS.gene60059:CDS	25.0%
!!	ATAACTAGATCTTGTTGTAA+CGG	+	chr6.1:35173112-35173131	None:intergenic	25.0%
!!!	ATGAATTTGTTATCTTCCAT+AGG	+	chr6.1:35172791-35172810	None:intergenic	25.0%
!!!	TGAATTTGTTATCTTCCATA+GGG	+	chr6.1:35172790-35172809	None:intergenic	25.0%
	ATGGAGAACCTAAACATTAA+TGG	-	chr6.1:35172605-35172624	MS.gene60059:CDS	30.0%
	CATGAATTCTACGGAAATAT+GGG	-	chr6.1:35172931-35172950	MS.gene60059:CDS	30.0%
	TCGATGAGGAAAACAATTAT+GGG	-	chr6.1:35173038-35173057	MS.gene60059:CDS	30.0%
	TTCGATGAGGAAAACAATTA+TGG	-	chr6.1:35173037-35173056	MS.gene60059:CDS	30.0%
	TTGCTAGATTTATGTCTTGT+TGG	-	chr6.1:35172674-35172693	MS.gene60059:CDS	30.0%
!	ACTTGATCATCTTTTCAATG+TGG	+	chr6.1:35173018-35173037	None:intergenic	30.0%
!	ATTCTGAAGAAAGTCGTAAT+TGG	-	chr6.1:35172887-35172906	MS.gene60059:CDS	30.0%
!	TTCTGAAGAAAGTCGTAATT+GGG	-	chr6.1:35172888-35172907	MS.gene60059:CDS	30.0%
	AAAGATGATCAAGTTCGATG+AGG	-	chr6.1:35173024-35173043	MS.gene60059:CDS	35.0%
	AGTCATTCAATGGCAATTGA+TGG	-	chr6.1:35173414-35173433	MS.gene60059:CDS	35.0%
	ATATTGTTGTCGAGTAACCA+GGG	+	chr6.1:35172863-35172882	None:intergenic	35.0%
	CATGGTCACCATTAATGTTT+AGG	+	chr6.1:35172616-35172635	None:intergenic	35.0%
	CCATATTTCCGTAGAATTCA+TGG	+	chr6.1:35172933-35172952	None:intergenic	35.0%
	CCATGAATTCTACGGAAATA+TGG	-	chr6.1:35172930-35172949	MS.gene60059:CDS	35.0%
	CTAAACATTAATGGTGACCA+TGG	-	chr6.1:35172614-35172633	MS.gene60059:CDS	35.0%
	TCTGAAGAAAGTCGTAATTG+GGG	-	chr6.1:35172889-35172908	MS.gene60059:CDS	35.0%
	TTATCTTCCATAGGGATTAG+AGG	+	chr6.1:35172782-35172801	None:intergenic	35.0%
!!	ATTAGAGGCATTTGGTACTT+TGG	+	chr6.1:35172767-35172786	None:intergenic	35.0%
	ACAAGATCTAGTTATCGAGC+AGG	-	chr6.1:35173117-35173136	MS.gene60059:CDS	40.0%
	CATATTGTTGTCGAGTAACC+AGG	+	chr6.1:35172864-35172883	None:intergenic	40.0%
	GACGATGTCCATGAATTCTA+CGG	-	chr6.1:35172922-35172941	MS.gene60059:CDS	40.0%
	GTTTGGATTCATGGAAGACA+AGG	-	chr6.1:35172973-35172992	MS.gene60059:CDS	40.0%
	TATTGTTGTCGAGTAACCAG+GGG	+	chr6.1:35172862-35172881	None:intergenic	40.0%
	TGCAAGCAGCGAGAAAAAAT+GGG	-	chr6.1:35173258-35173277	MS.gene60059:CDS	40.0%
	TGTGCTGAGAATAATTCCGT+TGG	-	chr6.1:35173291-35173310	MS.gene60059:CDS	40.0%
!	CATGGTCCAGCTTTTCTTAA+AGG	-	chr6.1:35172808-35172827	MS.gene60059:CDS	40.0%
!	GATCCTCCTTTAAGAAAAGC+TGG	+	chr6.1:35172817-35172836	None:intergenic	40.0%
!!	TGCTTTGTGTGTGGTGTTAT+AGG	-	chr6.1:35173193-35173212	MS.gene60059:CDS	40.0%
	AAAACAATTATGGGCCGTGG+AGG	-	chr6.1:35173047-35173066	MS.gene60059:CDS	45.0%
	AATTCTGCCTCAGTGAGGTT+CGG	+	chr6.1:35172656-35172675	None:intergenic	45.0%
	AGATCTAGTTATCGAGCAGG+AGG	-	chr6.1:35173120-35173139	MS.gene60059:CDS	45.0%
	AGGAAAACAATTATGGGCCG+TGG	-	chr6.1:35173044-35173063	MS.gene60059:CDS	45.0%
	CAAAAATTCTGGTGGCAGGA+AGG	-	chr6.1:35173359-35173378	MS.gene60059:CDS	45.0%
	CAAGCAGCGAGAAAAAATGG+GGG	-	chr6.1:35173260-35173279	MS.gene60059:CDS	45.0%
	CCAAATGCCTCTAATCCCTA+TGG	-	chr6.1:35172772-35172791	MS.gene60059:CDS	45.0%
	CCATGAATCCAAACAGCAAC+TGG	+	chr6.1:35172967-35172986	None:intergenic	45.0%
	CGGAATTATTCTCAGCACAG+AGG	+	chr6.1:35173290-35173309	None:intergenic	45.0%
	CTGCAAGCAGCGAGAAAAAA+TGG	-	chr6.1:35173257-35173276	MS.gene60059:CDS	45.0%
	GCAAGCAGCGAGAAAAAATG+GGG	-	chr6.1:35173259-35173278	MS.gene60059:CDS	45.0%
	GCTGAGAATAATTCCGTTGG+TGG	-	chr6.1:35173294-35173313	MS.gene60059:CDS	45.0%
	GGCAAATTCTGCTTTGTGTG+TGG	-	chr6.1:35173184-35173203	MS.gene60059:CDS	45.0%
	GTCACAAAGAGGCAAGCAAA+TGG	-	chr6.1:35173319-35173338	MS.gene60059:CDS	45.0%
!	CAGTTTCACCAGTTGCTGTT+TGG	-	chr6.1:35172956-35172975	MS.gene60059:CDS	45.0%
!	GAGAATAATTCCGTTGGTGG+TGG	-	chr6.1:35173297-35173316	MS.gene60059:CDS	45.0%
!	GGTCCAGCTTTTCTTAAAGG+AGG	-	chr6.1:35172811-35172830	MS.gene60059:CDS	45.0%
!	TGCCACCAGAATTTTTGCTC+CGG	+	chr6.1:35173356-35173375	None:intergenic	45.0%
!	TTTATGTCTTGTTGGCCGAG+TGG	-	chr6.1:35172682-35172701	MS.gene60059:CDS	45.0%
!!	CCAGTTGCTGTTTGGATTCA+TGG	-	chr6.1:35172964-35172983	MS.gene60059:CDS	45.0%
!!	CCATAGGGATTAGAGGCATT+TGG	+	chr6.1:35172775-35172794	None:intergenic	45.0%
!!	GTGTGGTGTTATAGGTCACT+CGG	-	chr6.1:35173201-35173220	MS.gene60059:CDS	45.0%
	AAATCCTTGAACGTAGCCCC+TGG	-	chr6.1:35172843-35172862	MS.gene60059:CDS	50.0%
	CTAGCAATTCTGCCTCAGTG+AGG	+	chr6.1:35172661-35172680	None:intergenic	50.0%
	CTAGTTATCGAGCAGGAGGA+AGG	-	chr6.1:35173124-35173143	MS.gene60059:CDS	50.0%
	CTCTTTGTGACCACCACCAA+CGG	+	chr6.1:35173310-35173329	None:intergenic	50.0%
	GACAAGGTTCGTGCTCTCAT+TGG	-	chr6.1:35172989-35173008	MS.gene60059:CDS	50.0%
	GAGGCAAGCAAATGGATCGT+TGG	-	chr6.1:35173327-35173346	MS.gene60059:CDS	50.0%
	GCAAGCAAATGGATCGTTGG+TGG	-	chr6.1:35173330-35173349	MS.gene60059:CDS	50.0%
	GGAGCAAAAATTCTGGTGGC+AGG	-	chr6.1:35173355-35173374	MS.gene60059:CDS	50.0%
	TGTTCACTTAGCCACGCTTG+AGG	-	chr6.1:35172718-35172737	MS.gene60059:CDS	50.0%
!	CATGCTTGTTAACCGCCACT+CGG	+	chr6.1:35172700-35172719	None:intergenic	50.0%
!!	AGGCAAGGCTTGGTCTTATG+TGG	-	chr6.1:35172738-35172757	MS.gene60059:CDS	50.0%
!!	GGCAAGGCTTGGTCTTATGT+GGG	-	chr6.1:35172739-35172758	MS.gene60059:CDS	50.0%
	ACAAAGGCCGAACCTCACTG+AGG	-	chr6.1:35172646-35172665	MS.gene60059:CDS	55.0%
	ACAATTATGGGCCGTGGAGG+AGG	-	chr6.1:35173050-35173069	MS.gene60059:CDS	55.0%
	ACCATGGCGCTGCATCACAA+AGG	-	chr6.1:35172630-35172649	MS.gene60059:CDS	55.0%
	ACCCTCATGAACCTCCTCCA+CGG	+	chr6.1:35173064-35173083	None:intergenic	55.0%
	ACTTAGCCACGCTTGAGGCA+AGG	-	chr6.1:35172723-35172742	MS.gene60059:CDS	55.0%
	CCTCCACCGCAATTTCCACT+CGG	+	chr6.1:35173087-35173106	None:intergenic	55.0%
	CTGGTGGCAGGAAGGAAGAA+GGG	-	chr6.1:35173367-35173386	MS.gene60059:CDS	55.0%
	GAGGGTCCGAGTGGAAATTG+CGG	-	chr6.1:35173078-35173097	MS.gene60059:CDS	55.0%
	GGCCGGAGCAAAAATTCTGG+TGG	-	chr6.1:35173351-35173370	MS.gene60059:CDS	55.0%
	GGTGGCCGGAGCAAAAATTC+TGG	-	chr6.1:35173348-35173367	MS.gene60059:CDS	55.0%
	GTAACCAGGGGCTACGTTCA+AGG	+	chr6.1:35172850-35172869	None:intergenic	55.0%
	TCTGGTGGCAGGAAGGAAGA+AGG	-	chr6.1:35173366-35173385	MS.gene60059:CDS	55.0%
!	ATGTCTTGTTGGCCGAGTGG+CGG	-	chr6.1:35172685-35172704	MS.gene60059:CDS	55.0%
!!	CGTTGGTGGTGGTCACAAAG+AGG	-	chr6.1:35173308-35173327	MS.gene60059:CDS	55.0%
!!	GCAAATGGATCGTTGGTGGC+CGG	-	chr6.1:35173334-35173353	MS.gene60059:CDS	55.0%
	ACCAAGCCTTGCCTCAAGCG+TGG	+	chr6.1:35172732-35172751	None:intergenic	60.0%
	CCGAGTGGAAATTGCGGTGG+AGG	-	chr6.1:35173084-35173103	MS.gene60059:CDS	60.0%
	GAGGTTCATGAGGGTCCGAG+TGG	-	chr6.1:35173069-35173088	MS.gene60059:CDS	60.0%
	GCCGTGGAGGAGGTTCATGA+GGG	-	chr6.1:35173060-35173079	MS.gene60059:CDS	60.0%
	GCCTTTGTGATGCAGCGCCA+TGG	+	chr6.1:35172634-35172653	None:intergenic	60.0%
	GGTCCGAGTGGAAATTGCGG+TGG	-	chr6.1:35173081-35173100	MS.gene60059:CDS	60.0%
	GCCACGCTTGAGGCAAGGCT+TGG	-	chr6.1:35172728-35172747	MS.gene60059:CDS	65.0%
	GGCCGTGGAGGAGGTTCATG+AGG	-	chr6.1:35173059-35173078	MS.gene60059:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr6.1	gene	35172605	35173447	35172605	ID=MS.gene60059
chr6.1	mRNA	35172605	35173447	35172605	ID=MS.gene60059.t1;Parent=MS.gene60059
chr6.1	exon	35172605	35173447	35172605	ID=MS.gene60059.t1.exon1;Parent=MS.gene60059.t1
chr6.1	CDS	35172605	35173447	35172605	ID=cds.MS.gene60059.t1;Parent=MS.gene60059.t1

Gene Sequence

>MS.gene60059

ATGGAGAACCTAAACATTAATGGTGACCATGGCGCTGCATCACAAAGGCCGAACCTCACTGAGGCAGAATTGCTAGATTTATGTCTTGTTGGCCGAGTGGCGGTTAACAAGCATGTTCACTTAGCCACGCTTGAGGCAAGGCTTGGTCTTATGTGGGATCCAAAGTACCAAATGCCTCTAATCCCTATGGAAGATAACAAATTCATGGTCCAGCTTTTCTTAAAGGAGGATCTTGTCAAAATCCTTGAACGTAGCCCCTGGTTACTCGACAACAATATGATTATTCTGAAGAAAGTCGTAATTGGGGAGAATCATCTGACGATGTCCATGAATTCTACGGAAATATGGGCTCAGTTTCACCAGTTGCTGTTTGGATTCATGGAAGACAAGGTTCGTGCTCTCATTGGAAGCCACATTGAAAAGATGATCAAGTTCGATGAGGAAAACAATTATGGGCCGTGGAGGAGGTTCATGAGGGTCCGAGTGGAAATTGCGGTGGAGGAGCCGTTACAACAAGATCTAGTTATCGAGCAGGAGGAAGGTAACAACATAAGACTTGTTGTTAAGTATGAGAAATTAGGCAAATTCTGCTTTGTGTGTGGTGTTATAGGTCACTCGGAAAATTATTGCAGTGATAAATTTGAATACGGTTCTGCAAGCAGCGAGAAAAAATGGGGGCTTACCTCTGTGCTGAGAATAATTCCGTTGGTGGTGGTCACAAAGAGGCAAGCAAATGGATCGTTGGTGGCCGGAGCAAAAATTCTGGTGGCAGGAAGGAAGAAGGGACAACCATTAATGACAATCAAAATAGTCATTCAATGGCAATTGATGGTTGAAACATAA

Protein sequence

>MS.gene60059.t1

MENLNINGDHGAASQRPNLTEAELLDLCLVGRVAVNKHVHLATLEARLGLMWDPKYQMPLIPMEDNKFMVQLFLKEDLVKILERSPWLLDNNMIILKKVVIGENHLTMSMNSTEIWAQFHQLLFGFMEDKVRALIGSHIEKMIKFDEENNYGPWRRFMRVRVEIAVEEPLQQDLVIEQEEGNNIRLVVKYEKLGKFCFVCGVIGHSENYCSDKFEYGSASSEKKWGLTSVLRIIPLVVVTKRQANGSLVAGAKILVAGRKKGQPLMTIKIVIQWQLMVET