AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene018196


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene018196.t1 MTR_8g463110 57.292 96 39 1 95 188 24 119 3.54e-21 90.5
MS.gene018196.t1 MTR_3g053920 39.735 151 50 2 15 129 135 280 1.34e-20 87.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 61 sgRNAs with CRISPR-Local

Find 79 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CATCGGATTCACTTGAAAAT+AGG 0.183920 6.3:-12258715 None:intergenic
CCGGACCGTCCATTGGTTCT+TGG 0.189071 6.3:+12258680 MS.gene018196:CDS
CGGATGATGGAGTCCGTAAC+TGG 0.279694 6.3:+12258328 MS.gene018196:CDS
TGTCACAGGTGTGGAGTTAA+TGG 0.284861 6.3:+12258264 MS.gene018196:intron
GAATCCAGCTCGAACAATTC+AGG 0.289771 6.3:-12258306 None:intergenic
CAAAGGGACGGTGACATCTA+TGG 0.341791 6.3:-12258163 None:intergenic
CTGCTGCCCTCCTCTTCTTC+AGG 0.346225 6.3:+12258635 MS.gene018196:CDS
ATCGGATTCACTTGAAAATA+GGG 0.387354 6.3:-12258714 None:intergenic
TGAATTGTTCGAGCTGGATT+CGG 0.402545 6.3:+12258308 MS.gene018196:CDS
TTCGAGCTGGATTCGGATGA+TGG 0.403028 6.3:+12258315 MS.gene018196:CDS
TACGGCTTCGAATCTGCTTC+CGG 0.410575 6.3:+12258661 MS.gene018196:CDS
GGAGAATCATTTGCCGCTGA+TGG 0.428507 6.3:+12258803 MS.gene018196:CDS
GCAGAGGTGTCTCGACCAAC+AGG 0.452398 6.3:-12258466 None:intergenic
CTGAAGAAGAGGAGGGCAGC+AGG 0.464221 6.3:-12258634 None:intergenic
GTATGTGGTGGCATGGCAGC+AGG 0.480468 6.3:-12258424 None:intergenic
TGCCACCAATCGTTGGCTAC+AGG 0.485246 6.3:+12258393 MS.gene018196:CDS
CTCCTCTTCTTCAGGTACTA+CGG 0.489884 6.3:+12258643 MS.gene018196:CDS
TCTGCTTCCGGACCGTCCAT+TGG 0.500948 6.3:+12258673 MS.gene018196:CDS
GAGAATCATTTGCCGCTGAT+GGG 0.504552 6.3:+12258804 MS.gene018196:CDS
GGTGCTGCTGCTGCTCCTGT+TGG 0.507384 6.3:+12258451 MS.gene018196:CDS
TTCCTCATGCACATCATCAT+CGG 0.509460 6.3:-12258732 None:intergenic
ATGCCCTGAATTGTTCGAGC+TGG 0.514593 6.3:+12258302 MS.gene018196:CDS
ATCCTGTAGCCAACGATTGG+TGG 0.519843 6.3:-12258395 None:intergenic
GGTGGCATGGCAGCAGGGAT+AGG 0.521932 6.3:-12258418 None:intergenic
GGATGATGGAGTCCGTAACT+GGG 0.524928 6.3:+12258329 MS.gene018196:CDS
CCAAGAACCAATGGACGGTC+CGG 0.530560 6.3:-12258680 None:intergenic
AATCCAGCTCGAACAATTCA+GGG 0.530855 6.3:-12258305 None:intergenic
GCAGCAGCACCGTGAGTATG+TGG 0.535545 6.3:-12258439 None:intergenic
GAAGAAAAGAAAGCGTATGC+TGG 0.536652 6.3:+12258769 MS.gene018196:CDS
CGTATGCTGGCCCTTCAGAA+TGG 0.538271 6.3:+12258782 MS.gene018196:CDS
AAACTGGCGTCACAAAGGAT+TGG 0.539584 6.3:+12258364 MS.gene018196:CDS
TCACTTGAAAATAGGGCAGC+GGG 0.540835 6.3:-12258707 None:intergenic
CAAATGATTCTCCATTCTGA+AGG 0.547464 6.3:-12258793 None:intergenic
ATGATGGAGTCCGTAACTGG+GGG 0.549135 6.3:+12258331 MS.gene018196:CDS
CTCCTATATGCGTATCAAGA+TGG 0.552699 6.3:+12258140 None:intergenic
AGGATCCTGTAGCCAACGAT+TGG 0.553045 6.3:-12258398 None:intergenic
AGCCGTAGTACCTGAAGAAG+AGG 0.566371 6.3:-12258645 None:intergenic
GAACTGCTGCCACCAATCGT+TGG 0.566578 6.3:+12258386 MS.gene018196:CDS
GATGATGTGCATGAGGAAAA+CGG 0.569015 6.3:+12258737 MS.gene018196:CDS
TTCACTTGAAAATAGGGCAG+CGG 0.575426 6.3:-12258708 None:intergenic
GATGATGGAGTCCGTAACTG+GGG 0.579407 6.3:+12258330 MS.gene018196:CDS
CGTAGTACCTGAAGAAGAGG+AGG 0.582803 6.3:-12258642 None:intergenic
ATTTCTCATTTCCCCATCAG+CGG 0.586243 6.3:-12258816 None:intergenic
GGATCAAATGTTAGTTGTCG+AGG 0.587387 6.3:+12258848 MS.gene018196:CDS
AGAATCATTTGCCGCTGATG+GGG 0.597704 6.3:+12258805 MS.gene018196:CDS
GGTCGAGACACCTCTGCTGA+AGG 0.602949 6.3:+12258472 MS.gene018196:CDS
GCGGGTAAACCAAGAACCAA+TGG 0.605853 6.3:-12258689 None:intergenic
GCCATGCCACCACATACTCA+CGG 0.616321 6.3:+12258430 MS.gene018196:CDS
CAAAAGCACCATTACTAGCA+CGG 0.626040 6.3:-12258203 None:intergenic
TGGCGTGTCCGTGCTAGTAA+TGG 0.627822 6.3:+12258195 MS.gene018196:CDS
ACCGTGAGTATGTGGTGGCA+TGG 0.637379 6.3:-12258431 None:intergenic
AAATGATTCTCCATTCTGAA+GGG 0.637855 6.3:-12258792 None:intergenic
ATCTCAAACTGGCGTCACAA+AGG 0.639822 6.3:+12258359 MS.gene018196:CDS
GCAGCACCGTGAGTATGTGG+TGG 0.640577 6.3:-12258436 None:intergenic
TGTGCATGAGGAAAACGGCT+CGG 0.646428 6.3:+12258742 MS.gene018196:CDS
TGATGGAGTCCGTAACTGGG+GGG 0.653348 6.3:+12258332 MS.gene018196:CDS
GTAAACCAAGAACCAATGGA+CGG 0.668596 6.3:-12258685 None:intergenic
GTAGTACCTGAAGAAGAGGA+GGG 0.671357 6.3:-12258641 None:intergenic
AGTGCATCTACCTTCAGCAG+AGG 0.674381 6.3:-12258482 None:intergenic
ATCCGATGATGATGTGCATG+AGG 0.687768 6.3:+12258730 MS.gene018196:CDS
TATGTGGTGGCATGGCAGCA+GGG 0.695816 6.3:-12258423 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAAATGAGAAATTTTTTAAT+AGG + chr6.3:12258827-12258846 MS.gene018196:CDS 15.0%
!!! GATTTTAAATACGAAAAACT+TGG + chr6.3:12258234-12258253 MS.gene018196:intron 20.0%
!!! GTTACAAGTTTTAATCTGTA+TGG + chr6.3:12258505-12258524 MS.gene018196:intron 25.0%
AAATGATTCTCCATTCTGAA+GGG - chr6.3:12258795-12258814 None:intergenic 30.0%
ATCGGATTCACTTGAAAATA+GGG - chr6.3:12258717-12258736 None:intergenic 30.0%
GTGTTAGCTAAAAAAGGTAA+CGG + chr6.3:12258587-12258606 MS.gene018196:intron 30.0%
!!! TTAGTTGTCGAGGTATTTTT+TGG + chr6.3:12258858-12258877 MS.gene018196:CDS 30.0%
CAAATGATTCTCCATTCTGA+AGG - chr6.3:12258796-12258815 None:intergenic 35.0%
CATCGGATTCACTTGAAAAT+AGG - chr6.3:12258718-12258737 None:intergenic 35.0%
! AATGCGGTGTTAGCTAAAAA+AGG + chr6.3:12258581-12258600 MS.gene018196:intron 35.0%
AATCCAGCTCGAACAATTCA+GGG - chr6.3:12258308-12258327 None:intergenic 40.0%
ATTTCTCATTTCCCCATCAG+CGG - chr6.3:12258819-12258838 None:intergenic 40.0%
GATGATGTGCATGAGGAAAA+CGG + chr6.3:12258737-12258756 MS.gene018196:CDS 40.0%
GGATCAAATGTTAGTTGTCG+AGG + chr6.3:12258848-12258867 MS.gene018196:CDS 40.0%
GTAAACCAAGAACCAATGGA+CGG - chr6.3:12258688-12258707 None:intergenic 40.0%
TGAATTGTTCGAGCTGGATT+CGG + chr6.3:12258308-12258327 MS.gene018196:CDS 40.0%
TTCACTTGAAAATAGGGCAG+CGG - chr6.3:12258711-12258730 None:intergenic 40.0%
TTCCTCATGCACATCATCAT+CGG - chr6.3:12258735-12258754 None:intergenic 40.0%
TTTAGCTAACACCGCATTCT+GGG - chr6.3:12258579-12258598 None:intergenic 40.0%
! GAAGAAAAGAAAGCGTATGC+TGG + chr6.3:12258769-12258788 MS.gene018196:CDS 40.0%
!! CAAAAGCACCATTACTAGCA+CGG - chr6.3:12258206-12258225 None:intergenic 40.0%
!! TTTTAGCTAACACCGCATTC+TGG - chr6.3:12258580-12258599 None:intergenic 40.0%
!!! AACTTGGTGTCTTTTGTCAC+AGG + chr6.3:12258250-12258269 MS.gene018196:intron 40.0%
!!! ACACGCCACTCTTTTTTCAA+AGG - chr6.3:12258183-12258202 None:intergenic 40.0%
!!! CACGCCACTCTTTTTTCAAA+GGG - chr6.3:12258182-12258201 None:intergenic 40.0%
!!! CCACTCTTTTTTCAAAGGGA+CGG - chr6.3:12258178-12258197 None:intergenic 40.0%
AAACTGGCGTCACAAAGGAT+TGG + chr6.3:12258364-12258383 MS.gene018196:CDS 45.0%
AGAATCATTTGCCGCTGATG+GGG + chr6.3:12258805-12258824 MS.gene018196:CDS 45.0%
ATCTCAAACTGGCGTCACAA+AGG + chr6.3:12258359-12258378 MS.gene018196:CDS 45.0%
CTCCTCTTCTTCAGGTACTA+CGG + chr6.3:12258643-12258662 MS.gene018196:CDS 45.0%
GAATCCAGCTCGAACAATTC+AGG - chr6.3:12258309-12258328 None:intergenic 45.0%
GAGAATCATTTGCCGCTGAT+GGG + chr6.3:12258804-12258823 MS.gene018196:CDS 45.0%
GTAGTACCTGAAGAAGAGGA+GGG - chr6.3:12258644-12258663 None:intergenic 45.0%
TCACTTGAAAATAGGGCAGC+GGG - chr6.3:12258710-12258729 None:intergenic 45.0%
TGTCACAGGTGTGGAGTTAA+TGG + chr6.3:12258264-12258283 MS.gene018196:intron 45.0%
! ATCCGATGATGATGTGCATG+AGG + chr6.3:12258730-12258749 MS.gene018196:CDS 45.0%
! CCGTCCCTTTGAAAAAAGAG+TGG + chr6.3:12258175-12258194 MS.gene018196:CDS 45.0%
AGCAAGAACATCGTGTCGCA+TGG - chr6.3:12258555-12258574 None:intergenic 50.0%
AGCCGTAGTACCTGAAGAAG+AGG - chr6.3:12258648-12258667 None:intergenic 50.0%
AGGATCCTGTAGCCAACGAT+TGG - chr6.3:12258401-12258420 None:intergenic 50.0%
AGTGCATCTACCTTCAGCAG+AGG - chr6.3:12258485-12258504 None:intergenic 50.0%
ATCCTGTAGCCAACGATTGG+TGG - chr6.3:12258398-12258417 None:intergenic 50.0%
ATGCCCTGAATTGTTCGAGC+TGG + chr6.3:12258302-12258321 MS.gene018196:CDS 50.0%
CAAAGGGACGGTGACATCTA+TGG - chr6.3:12258166-12258185 None:intergenic 50.0%
CGTAGTACCTGAAGAAGAGG+AGG - chr6.3:12258645-12258664 None:intergenic 50.0%
GCGGGTAAACCAAGAACCAA+TGG - chr6.3:12258692-12258711 None:intergenic 50.0%
GGAGAATCATTTGCCGCTGA+TGG + chr6.3:12258803-12258822 MS.gene018196:CDS 50.0%
TACGGCTTCGAATCTGCTTC+CGG + chr6.3:12258661-12258680 MS.gene018196:CDS 50.0%
TGTGCATGAGGAAAACGGCT+CGG + chr6.3:12258742-12258761 MS.gene018196:CDS 50.0%
TGTTCTTGCTGCCCAGAATG+CGG + chr6.3:12258565-12258584 MS.gene018196:intron 50.0%
TTCGAGCTGGATTCGGATGA+TGG + chr6.3:12258315-12258334 MS.gene018196:CDS 50.0%
! ATGATGGAGTCCGTAACTGG+GGG + chr6.3:12258331-12258350 MS.gene018196:CDS 50.0%
! GGTGTCTTTTGTCACAGGTG+TGG + chr6.3:12258255-12258274 MS.gene018196:intron 50.0%
!! GATGATGGAGTCCGTAACTG+GGG + chr6.3:12258330-12258349 MS.gene018196:CDS 50.0%
!! GGATGATGGAGTCCGTAACT+GGG + chr6.3:12258329-12258348 MS.gene018196:CDS 50.0%
ACCGTGAGTATGTGGTGGCA+TGG - chr6.3:12258434-12258453 None:intergenic 55.0%
CCAAGAACCAATGGACGGTC+CGG - chr6.3:12258683-12258702 None:intergenic 55.0%
CGTATGCTGGCCCTTCAGAA+TGG + chr6.3:12258782-12258801 MS.gene018196:CDS 55.0%
GAACTGCTGCCACCAATCGT+TGG + chr6.3:12258386-12258405 MS.gene018196:CDS 55.0%
GAGATACAGCCCCCCAGTTA+CGG - chr6.3:12258344-12258363 None:intergenic 55.0%
GCATGGCAGCGAGTTGTGTT+TGG - chr6.3:12258538-12258557 None:intergenic 55.0%
GCCATGCCACCACATACTCA+CGG + chr6.3:12258430-12258449 MS.gene018196:CDS 55.0%
TATGTGGTGGCATGGCAGCA+GGG - chr6.3:12258426-12258445 None:intergenic 55.0%
TGATGGAGTCCGTAACTGGG+GGG + chr6.3:12258332-12258351 MS.gene018196:CDS 55.0%
TGGCGTGTCCGTGCTAGTAA+TGG + chr6.3:12258195-12258214 MS.gene018196:CDS 55.0%
TGGGGGGCTGTATCTCAAAC+TGG + chr6.3:12258348-12258367 MS.gene018196:CDS 55.0%
! TGCCACCAATCGTTGGCTAC+AGG + chr6.3:12258393-12258412 MS.gene018196:CDS 55.0%
!! CGGATGATGGAGTCCGTAAC+TGG + chr6.3:12258328-12258347 MS.gene018196:CDS 55.0%
CTGAAGAAGAGGAGGGCAGC+AGG - chr6.3:12258637-12258656 None:intergenic 60.0%
CTGCTGCCCTCCTCTTCTTC+AGG + chr6.3:12258635-12258654 MS.gene018196:CDS 60.0%
GCAGCACCGTGAGTATGTGG+TGG - chr6.3:12258439-12258458 None:intergenic 60.0%
GCAGCAGCACCGTGAGTATG+TGG - chr6.3:12258442-12258461 None:intergenic 60.0%
GGTCGAGACACCTCTGCTGA+AGG + chr6.3:12258472-12258491 MS.gene018196:CDS 60.0%
GTATGTGGTGGCATGGCAGC+AGG - chr6.3:12258427-12258446 None:intergenic 60.0%
TCTGCTTCCGGACCGTCCAT+TGG + chr6.3:12258673-12258692 MS.gene018196:CDS 60.0%
!! CCGGACCGTCCATTGGTTCT+TGG + chr6.3:12258680-12258699 MS.gene018196:CDS 60.0%
!! GCAGAGGTGTCTCGACCAAC+AGG - chr6.3:12258469-12258488 None:intergenic 60.0%
GGTGGCATGGCAGCAGGGAT+AGG - chr6.3:12258421-12258440 None:intergenic 65.0%
!! GGTGCTGCTGCTGCTCCTGT+TGG + chr6.3:12258451-12258470 MS.gene018196:CDS 65.0%


Chromosome Type Strat End Strand Name
chr6.3 gene 12258147 12258889 12258147 ID=MS.gene018196
chr6.3 mRNA 12258147 12258889 12258147 ID=MS.gene018196.t1;Parent=MS.gene018196
chr6.3 exon 12258147 12258216 12258147 ID=MS.gene018196.t1.exon1;Parent=MS.gene018196.t1
chr6.3 CDS 12258147 12258216 12258147 ID=cds.MS.gene018196.t1;Parent=MS.gene018196.t1
chr6.3 exon 12258272 12258493 12258272 ID=MS.gene018196.t1.exon2;Parent=MS.gene018196.t1
chr6.3 CDS 12258272 12258493 12258272 ID=cds.MS.gene018196.t1;Parent=MS.gene018196.t1
chr6.3 exon 12258603 12258889 12258603 ID=MS.gene018196.t1.exon3;Parent=MS.gene018196.t1
chr6.3 CDS 12258603 12258889 12258603 ID=cds.MS.gene018196.t1;Parent=MS.gene018196.t1
Gene Sequence

>MS.gene018196

ATGCGTATCAAGATGGCCATAGATGTCACCGTCCCTTTGAAAAAAGAGTGGCGTGTCCGTGCTAGTAATGGTGCTTTTGTTACTGTTGATTTTAAATACGAAAAACTTGGTGTCTTTTGTCACAGGTGTGGAGTTAATGGACATACAGACAAGATATGCCCTGAATTGTTCGAGCTGGATTCGGATGATGGAGTCCGTAACTGGGGGGCTGTATCTCAAACTGGCGTCACAAAGGATTGGAACTGCTGCCACCAATCGTTGGCTACAGGATCCTATCCCTGCTGCCATGCCACCACATACTCACGGTGCTGCTGCTGCTCCTGTTGGTCGAGACACCTCTGCTGAAGGTAGATGCACTGTTACAAGTTTTAATCTGTATGGCTGCTTTCCAAACACAACTCGCTGCCATGCGACACGATGTTCTTGCTGCCCAGAATGCGGTGTTAGCTAAAAAAGGTAACGGTGTCAATTCTGCTTGCAAAGTGCACCTGCTGCCCTCCTCTTCTTCAGGTACTACGGCTTCGAATCTGCTTCCGGACCGTCCATTGGTTCTTGGTTTACCCGCTGCCCTATTTTCAAGTGAATCCGATGATGATGTGCATGAGGAAAACGGCTCGGATTTGAAGAAAAGAAAGCGTATGCTGGCCCTTCAGAATGGAGAATCATTTGCCGCTGATGGGGAAATGAGAAATTTTTTAATAGGATCAAATGTTAGTTGTCGAGGTATTTTTTGGATGTCATAG

Protein sequence

>MS.gene018196.t1

MRIKMAIDVTVPLKKEWRVRASNGVELMDIQTRYALNCSSWIRMMESVTGGLYLKLASQRIGTAATNRWLQDPIPAAMPPHTHGAAAAPVGRDTSAEGNGVNSACKVHLLPSSSSGTTASNLLPDRPLVLGLPAALFSSESDDDVHEENGSDLKKRKRMLALQNGESFAADGEMRNFLIGSNVSCRGIFWMS