AlfalfaGEDB | MS.gene50392

M. sativa cultivar XinJiangDaYe / MS.gene50392

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50392.t1	AES70414.1	48.5	338	70	5	28	319	1	280	4.20E-72	282

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50392.t1	G7J017	48.5	338	70	5	28	319	1	280	3.1e-72	282.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene50392	MS.gene69584	PPI
MS.gene50392	MS.gene52349	PPI
MS.gene26038	MS.gene50392	PPI
MS.gene50392	MS.gene46353	PPI
MS.gene85308	MS.gene50392	PPI
MS.gene073573	MS.gene50392	PPI
MS.gene031967	MS.gene50392	PPI
MS.gene34812	MS.gene50392	PPI
MS.gene50392	MS.gene61651	PPI
MS.gene02896	MS.gene50392	PPI
MS.gene063214	MS.gene50392	PPI
MS.gene55682	MS.gene50392	PPI
MS.gene32415	MS.gene50392	PPI
MS.gene51799	MS.gene50392	PPI
MS.gene50392	MS.gene016719	PPI
MS.gene035626	MS.gene50392	PPI
MS.gene50392	MS.gene88393	PPI
MS.gene50392	MS.gene054508	PPI
MS.gene067340	MS.gene50392	PPI
MS.gene50392	MS.gene053373	PPI
MS.gene65623	MS.gene50392	PPI
MS.gene71675	MS.gene50392	PPI
MS.gene019177	MS.gene50392	PPI
MS.gene69495	MS.gene50392	PPI
MS.gene049512	MS.gene50392	PPI
MS.gene50392	MS.gene63150	PPI
MS.gene29406	MS.gene50392	PPI
MS.gene038623	MS.gene50392	PPI
MS.gene05357	MS.gene50392	PPI
MS.gene032311	MS.gene50392	PPI
MS.gene50392	MS.gene65474	PPI
MS.gene004417	MS.gene50392	PPI
MS.gene50392	MS.gene025401	PPI
MS.gene50392	MS.gene015093	PPI
MS.gene50392	MS.gene06164	PPI
MS.gene86849	MS.gene50392	PPI
MS.gene50392	MS.gene22730	PPI
MS.gene50392	MS.gene032299	PPI
MS.gene072852	MS.gene50392	PPI
MS.gene50392	MS.gene08606	PPI
MS.gene39956	MS.gene50392	PPI
MS.gene021968	MS.gene50392	PPI
MS.gene50392	MS.gene000640	PPI
MS.gene50392	MS.gene09517	PPI
MS.gene50392	MS.gene98756	PPI
MS.gene09589	MS.gene50392	PPI
MS.gene50392	MS.gene87845	PPI
MS.gene43393	MS.gene50392	PPI
MS.gene057828	MS.gene50392	PPI
MS.gene34197	MS.gene50392	PPI
MS.gene50392	MS.gene62249	PPI
MS.gene50392	MS.gene010290	PPI
MS.gene66650	MS.gene50392	PPI
MS.gene005120	MS.gene50392	PPI
MS.gene50392	MS.gene92517	PPI
MS.gene048072	MS.gene50392	PPI
MS.gene060386	MS.gene50392	PPI
MS.gene71588	MS.gene50392	PPI
MS.gene50392	MS.gene044729	PPI
MS.gene04749	MS.gene50392	PPI
MS.gene50392	MS.gene60390	PPI
MS.gene05436	MS.gene50392	PPI
MS.gene50392	MS.gene039692	PPI
MS.gene059736	MS.gene50392	PPI
MS.gene70932	MS.gene50392	PPI
MS.gene027169	MS.gene50392	PPI
MS.gene021965	MS.gene50392	PPI
MS.gene055683	MS.gene50392	PPI
MS.gene042614	MS.gene50392	PPI
MS.gene41643	MS.gene50392	PPI
MS.gene26844	MS.gene50392	PPI
MS.gene021555	MS.gene50392	PPI
MS.gene50392	MS.gene024297	PPI
MS.gene50392	MS.gene059269	PPI
MS.gene021647	MS.gene50392	PPI
MS.gene50392	MS.gene057934	PPI
MS.gene50392	MS.gene024841	PPI
MS.gene019165	MS.gene50392	PPI
MS.gene048706	MS.gene50392	PPI
MS.gene50392	MS.gene069786	PPI
MS.gene50392	MS.gene025158	PPI
MS.gene009121	MS.gene50392	PPI
MS.gene94654	MS.gene50392	PPI
MS.gene50392	MS.gene51799	PPI
MS.gene51804	MS.gene50392	PPI
MS.gene87845	MS.gene50392	PPI
MS.gene36069	MS.gene50392	PPI
MS.gene97410	MS.gene50392	PPI
MS.gene83772	MS.gene50392	PPI
MS.gene009213	MS.gene50392	PPI
MS.gene50392	MS.gene40469	PPI
MS.gene008927	MS.gene50392	PPI
MS.gene045614	MS.gene50392	PPI
MS.gene50392	MS.gene09023	PPI
MS.gene049561	MS.gene50392	PPI
MS.gene50392	MS.gene025987	PPI
MS.gene000634	MS.gene50392	PPI
MS.gene50392	MS.gene95960	PPI
MS.gene50392	MS.gene043140	PPI
MS.gene63762	MS.gene50392	PPI
MS.gene50392	MS.gene03149	PPI
MS.gene30208	MS.gene50392	PPI
MS.gene028716	MS.gene50392	PPI
MS.gene025873	MS.gene50392	PPI
MS.gene06164	MS.gene50392	PPI
MS.gene013676	MS.gene50392	PPI
MS.gene50392	MS.gene29321	PPI
MS.gene55209	MS.gene50392	PPI
MS.gene05358	MS.gene50392	PPI
MS.gene64178	MS.gene50392	PPI
MS.gene030090	MS.gene50392	PPI
MS.gene95561	MS.gene50392	PPI
MS.gene96174	MS.gene50392	PPI
MS.gene018196	MS.gene50392	PPI
MS.gene065627	MS.gene50392	PPI
MS.gene50392	MS.gene24278	PPI
MS.gene50392	MS.gene000379	PPI
MS.gene50392	MS.gene34618	PPI
MS.gene57019	MS.gene50392	PPI
MS.gene50392	MS.gene59460	PPI
MS.gene018745	MS.gene50392	PPI
MS.gene65546	MS.gene50392	PPI
MS.gene50392	MS.gene060773	PPI
MS.gene000506	MS.gene50392	PPI
MS.gene03936	MS.gene50392	PPI
MS.gene50392	MS.gene08713	PPI
MS.gene038098	MS.gene50392	PPI
MS.gene50392	MS.gene41393	PPI
MS.gene60059	MS.gene50392	PPI
MS.gene22111	MS.gene50392	PPI
MS.gene50392	MS.gene24070	PPI
MS.gene28545	MS.gene50392	PPI
MS.gene28718	MS.gene50392	PPI
MS.gene055560	MS.gene50392	PPI
MS.gene008275	MS.gene50392	PPI
MS.gene038757	MS.gene50392	PPI
MS.gene064966	MS.gene50392	PPI
MS.gene50392	MS.gene44144	PPI
MS.gene002590	MS.gene50392	PPI
MS.gene50392	MS.gene027169	PPI
MS.gene40828	MS.gene50392	PPI
MS.gene43924	MS.gene50392	PPI
MS.gene50392	MS.gene004417	PPI
MS.gene046089	MS.gene50392	PPI
MS.gene047954	MS.gene50392	PPI
MS.gene35175	MS.gene50392	PPI
MS.gene50392	MS.gene019177	PPI
MS.gene036423	MS.gene50392	PPI
MS.gene054652	MS.gene50392	PPI
MS.gene013644	MS.gene50392	PPI
MS.gene50392	MS.gene009213	PPI
MS.gene72520	MS.gene50392	PPI
MS.gene50392	MS.gene98127	PPI
MS.gene66651	MS.gene50392	PPI
MS.gene50392	MS.gene003264	PPI
MS.gene96109	MS.gene50392	PPI
MS.gene50392	MS.gene37537	PPI
MS.gene35202	MS.gene50392	PPI
MS.gene051449	MS.gene50392	PPI
MS.gene50392	MS.gene42010	PPI
MS.gene63743	MS.gene50392	PPI
MS.gene50392	MS.gene27329	PPI
MS.gene48477	MS.gene50392	PPI
MS.gene045875	MS.gene50392	PPI
MS.gene50392	MS.gene89815	PPI
MS.gene50392	MS.gene016632	PPI
MS.gene50392	MS.gene018474	PPI
MS.gene50392	MS.gene86868	PPI
MS.gene30136	MS.gene50392	PPI
MS.gene008861	MS.gene50392	PPI
MS.gene61452	MS.gene50392	PPI
MS.gene73261	MS.gene50392	PPI
MS.gene024297	MS.gene50392	PPI
MS.gene83979	MS.gene50392	PPI
MS.gene50392	MS.gene96163	PPI
MS.gene016102	MS.gene50392	PPI
MS.gene46941	MS.gene50392	PPI
MS.gene96541	MS.gene50392	PPI
MS.gene85295	MS.gene50392	PPI
MS.gene47792	MS.gene50392	PPI
MS.gene46948	MS.gene50392	PPI
MS.gene98471	MS.gene50392	PPI
MS.gene012019	MS.gene50392	PPI
MS.gene50392	MS.gene34463	PPI
MS.gene93396	MS.gene50392	PPI
MS.gene074257	MS.gene50392	PPI
MS.gene50392	MS.gene61499	PPI
MS.gene05231	MS.gene50392	PPI
MS.gene010656	MS.gene50392	PPI
MS.gene000629	MS.gene50392	PPI
MS.gene50392	MS.gene81194	PPI
MS.gene059269	MS.gene50392	PPI
MS.gene40469	MS.gene50392	PPI
MS.gene041264	MS.gene50392	PPI
MS.gene41979	MS.gene50392	PPI
MS.gene024522	MS.gene50392	PPI
MS.gene64130	MS.gene50392	PPI
MS.gene05319	MS.gene50392	PPI
MS.gene50392	MS.gene038394	PPI
MS.gene56991	MS.gene50392	PPI
MS.gene050679	MS.gene50392	PPI
MS.gene50392	MS.gene019001	PPI
MS.gene85538	MS.gene50392	PPI
MS.gene20246	MS.gene50392	PPI
MS.gene50392	MS.gene53904	PPI
MS.gene055223	MS.gene50392	PPI
MS.gene50392	MS.gene73261	PPI
MS.gene50392	MS.gene025675	PPI
MS.gene053747	MS.gene50392	PPI
MS.gene50392	MS.gene055546	PPI
MS.gene65471	MS.gene50392	PPI
MS.gene50392	MS.gene55209	PPI
MS.gene061850	MS.gene50392	PPI
MS.gene50392	MS.gene022929	PPI
MS.gene073582	MS.gene50392	PPI
MS.gene058889	MS.gene50392	PPI
MS.gene98616	MS.gene50392	PPI
MS.gene50392	MS.gene068629	PPI
MS.gene50392	MS.gene009781	PPI
MS.gene042027	MS.gene50392	PPI
MS.gene69490	MS.gene50392	PPI
MS.gene50392	MS.gene048342	PPI
MS.gene50392	MS.gene069624	PPI
MS.gene015248	MS.gene50392	PPI
MS.gene50392	MS.gene21066	PPI
MS.gene80362	MS.gene50392	PPI
MS.gene50392	MS.gene013890	PPI
MS.gene44083	MS.gene50392	PPI
MS.gene013193	MS.gene50392	PPI
MS.gene04970	MS.gene50392	PPI
MS.gene08106	MS.gene50392	PPI
MS.gene58419	MS.gene50392	PPI
MS.gene50392	MS.gene028714	PPI
MS.gene50392	MS.gene048706	PPI
MS.gene50392	MS.gene69586	PPI
MS.gene50392	MS.gene45083	PPI
MS.gene50392	MS.gene049561	PPI
MS.gene50392	MS.gene008658	PPI
MS.gene23521	MS.gene50392	PPI
MS.gene63150	MS.gene50392	PPI
MS.gene50392	MS.gene49057	PPI
MS.gene86275	MS.gene50392	PPI
MS.gene50392	MS.gene048088	PPI
MS.gene87529	MS.gene50392	PPI
MS.gene024405	MS.gene50392	PPI
MS.gene012748	MS.gene50392	PPI
MS.gene04134	MS.gene50392	PPI
MS.gene50392	MS.gene08708	PPI
MS.gene062067	MS.gene50392	PPI
MS.gene57044	MS.gene50392	PPI
MS.gene89815	MS.gene50392	PPI
MS.gene028714	MS.gene50392	PPI
MS.gene049610	MS.gene50392	PPI
MS.gene017586	MS.gene50392	PPI
MS.gene038676	MS.gene50392	PPI
MS.gene060718	MS.gene50392	PPI
MS.gene50392	MS.gene012889	PPI
MS.gene71637	MS.gene50392	PPI
MS.gene50392	MS.gene000506	PPI
MS.gene042651	MS.gene50392	PPI
MS.gene05576	MS.gene50392	PPI
MS.gene52349	MS.gene50392	PPI
MS.gene50392	MS.gene028716	PPI
MS.gene50392	MS.gene74639	PPI
MS.gene50392	MS.gene071133	PPI
MS.gene50392	MS.gene60266	PPI
MS.gene98467	MS.gene50392	PPI
MS.gene50392	MS.gene54051	PPI
MS.gene50392	MS.gene22111	PPI
MS.gene037049	MS.gene50392	PPI
MS.gene034643	MS.gene50392	PPI
MS.gene037634	MS.gene50392	PPI
MS.gene50392	MS.gene37955	PPI
MS.gene50392	MS.gene010890	PPI
MS.gene50392	MS.gene84790	PPI
MS.gene016719	MS.gene50392	PPI
MS.gene071784	MS.gene50392	PPI
MS.gene061579	MS.gene50392	PPI
MS.gene76420	MS.gene50392	PPI
MS.gene071320	MS.gene50392	PPI
MS.gene42903	MS.gene50392	PPI
MS.gene50392	MS.gene060788	PPI
MS.gene81712	MS.gene50392	PPI
MS.gene50392	MS.gene77990	PPI
MS.gene026680	MS.gene50392	PPI
MS.gene26433	MS.gene50392	PPI
MS.gene50392	MS.gene062511	PPI
MS.gene50392	MS.gene027994	PPI
MS.gene048747	MS.gene50392	PPI
MS.gene22730	MS.gene50392	PPI
MS.gene50392	MS.gene007022	PPI
MS.gene042559	MS.gene50392	PPI
MS.gene22200	MS.gene50392	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene50392.t1	MTR_3g053920	50.301	332	73	5	28	319	1	280	1.49e-93	281
MS.gene50392.t1	MTR_2g061500	87.129	101	13	0	28	128	1	101	2.81e-60	191
MS.gene50392.t1	MTR_4g103530	61.702	94	11	1	82	150	1	94	7.80e-31	116
MS.gene50392.t1	MTR_8g463110	54.701	117	51	1	263	377	2	118	2.52e-25	108
MS.gene50392.t1	MTR_6g033215	31.214	173	92	2	56	201	122	294	1.03e-23	100
MS.gene50392.t1	MTR_0010s0370	33.708	178	81	5	35	179	4	177	7.06e-23	95.1
MS.gene50392.t1	MTR_0010s0350	33.708	178	81	5	35	179	4	177	7.06e-23	95.1
MS.gene50392.t1	MTR_7g011710	50.588	85	17	1	82	141	1	85	1.64e-20	87.8
MS.gene50392.t1	MTR_6g053230	28.070	171	96	5	11	178	13	159	1.45e-14	75.5
MS.gene50392.t1	MTR_1g036720	36.449	107	59	4	34	136	3	104	1.00e-11	61.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 95 sgRNAs with CRISPR-Local

Find 123 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAGCCAGCAGATGTATTT+TGG	0.145079	4.4:-79073521	None:intergenic
ATTCTTGAACACTGTCTTAT+TGG	0.223174	4.4:+79072724	MS.gene50392:CDS
AGACATACGCTTGCTGAAAT+AGG	0.265838	4.4:-79072779	None:intergenic
CCTATGCCTTGACCTACTTT+TGG	0.274303	4.4:-79072964	None:intergenic
CGAAAGGCAGACCGTGAATA+TGG	0.313876	4.4:-79072930	None:intergenic
CATCGACTTTGTGGTGAAAC+TGG	0.323345	4.4:-79072864	None:intergenic
CTGATGATGGCGTCCGCAAT+TGG	0.330887	4.4:+79073226	MS.gene50392:CDS
CCAAGTCGTCCTTTGGTGCT+TGG	0.331606	4.4:+79073577	MS.gene50392:CDS
CATATTCACGGTCTGCCTTT+CGG	0.343325	4.4:+79072931	MS.gene50392:CDS
CAGGTTCGGTTTCAAGGTTA+AGG	0.368229	4.4:-79072690	None:intergenic
TGCGTAACTGGTTTCAAGTC+AGG	0.379588	4.4:-79073252	None:intergenic
TTTAGCTAGCACCGCATTAT+GGG	0.386461	4.4:-79073473	None:intergenic
CAGTATCAGGTTCGGTTTCA+AGG	0.389842	4.4:-79072696	None:intergenic
TGATGATGGCGTCCGCAATT+GGG	0.393295	4.4:+79073227	MS.gene50392:CDS
GAGAAACACTTGCTGCTGAT+AGG	0.397052	4.4:+79073701	MS.gene50392:CDS
CCTGCTTCCAAGTCGTCCTT+TGG	0.398210	4.4:+79073570	MS.gene50392:CDS
GCAAGCGTATGTCTCGAGTA+TGG	0.401222	4.4:+79072788	MS.gene50392:CDS
TCAAGAATGGCAGCAGTATC+AGG	0.410843	4.4:-79072709	None:intergenic
CTCCTTCCCAGCCGATCTCA+TGG	0.419919	4.4:+79072615	MS.gene50392:CDS
TATGACAATTTCCATATTCA+CGG	0.420305	4.4:+79072919	MS.gene50392:CDS
TTCGAATTAGATTCTGATGA+TGG	0.422237	4.4:+79073213	MS.gene50392:CDS
TGTCACAGGTGTGGATTGAT+TGG	0.423731	4.4:+79073162	MS.gene50392:CDS
TGCTTCCAAAATACATCTGC+TGG	0.424718	4.4:+79073516	MS.gene50392:CDS
AGGTGAGACCGTCAAGGTTA+AGG	0.433080	4.4:-79072648	None:intergenic
AGGTTCGGTTTCAAGGTTAA+GGG	0.437088	4.4:-79072689	None:intergenic
GTGATCGTAACCTTCTTGCC+TGG	0.441945	4.4:-79072814	None:intergenic
CAAGGCATAGGAATCTTCTT+AGG	0.442305	4.4:+79072976	MS.gene50392:CDS
GAAGGATCCATGAGATCGGC+TGG	0.451674	4.4:-79072622	None:intergenic
TCATTAGATTCACTTGAGAA+TGG	0.464881	4.4:-79073613	None:intergenic
GTAACGGTCAGACACTGATT+TGG	0.466779	4.4:-79072836	None:intergenic
CGGTCTCACCTTGTCTGAAG+AGG	0.477786	4.4:+79072660	MS.gene50392:CDS
TTCAAATGTTAGTCACGGGG+GGG	0.495873	4.4:+79073747	MS.gene50392:CDS
TACGTGCAGCATCGACTTTG+TGG	0.498421	4.4:-79072873	None:intergenic
CCTTCCAACCTTAACCTTGA+CGG	0.500001	4.4:+79072640	MS.gene50392:CDS
CGTGCGCTGGCCGTTCAGAA+TGG	0.503482	4.4:+79073679	MS.gene50392:CDS
CCAAGCACCAAAGGACGACT+TGG	0.504912	4.4:-79073577	None:intergenic
CTGCTGGCTTCTTCTTCCAC+AGG	0.507471	4.4:+79073532	MS.gene50392:CDS
GATGATGGCGTCCGCAATTG+GGG	0.511063	4.4:+79073228	MS.gene50392:CDS
CACCTTCAGTAGCTGCAGTT+CGG	0.513939	4.4:-79073369	None:intergenic
GCAGGTAAGCCAAGCACCAA+AGG	0.518575	4.4:-79073586	None:intergenic
AGTAAGAAAAGCACTATTTG+TGG	0.521014	4.4:-79073106	None:intergenic
TAGGTTCAAATGTTAGTCAC+GGG	0.526713	4.4:+79073743	MS.gene50392:CDS
CTTTGTGGTGAAACTGGAAA+AGG	0.528156	4.4:-79072858	None:intergenic
CCAAAGGACGACTTGGAAGC+AGG	0.528272	4.4:-79073570	None:intergenic
TCACTTGAGAATGGAGCAGC+AGG	0.528382	4.4:-79073604	None:intergenic
AATAAGACAGTGTTCAAGAA+TGG	0.530523	4.4:-79072722	None:intergenic
AATGGCAGCAGTATCAGGTT+CGG	0.531216	4.4:-79072704	None:intergenic
GGATTCATCCAACCAAAAGT+AGG	0.533518	4.4:+79072952	MS.gene50392:CDS
ATCCATGAGATCGGCTGGGA+AGG	0.534590	4.4:-79072617	None:intergenic
TGCTGCACGTATTCTTGATG+AGG	0.537306	4.4:+79072885	MS.gene50392:CDS
GAAGAAAAGAAAGCGTGCGC+TGG	0.537727	4.4:+79073666	MS.gene50392:CDS
AGAAACACTTGCTGCTGATA+GGG	0.538425	4.4:+79073702	MS.gene50392:CDS
AACTGCAGCTACTGAAGGTG+TGG	0.539423	4.4:+79073372	MS.gene50392:CDS
ATGTCTCGAGTATGGAAGCC+AGG	0.542857	4.4:+79072796	MS.gene50392:CDS
GTATTCTTGATGAGGGTCCA+TGG	0.545558	4.4:+79072893	MS.gene50392:CDS
ACTTGCTGCTGATAGGGATG+TGG	0.547601	4.4:+79073708	MS.gene50392:CDS
CATGAGATCGGCTGGGAAGG+AGG	0.562053	4.4:-79072614	None:intergenic
GGTCTCACCTTGTCTGAAGA+GGG	0.564346	4.4:+79072661	MS.gene50392:CDS
TCAAGTCAGGTCCCCAATTG+CGG	0.565946	4.4:-79073239	None:intergenic
GTTCAAATGTTAGTCACGGG+GGG	0.572107	4.4:+79073746	MS.gene50392:CDS
CCGTCAAGGTTAAGGTTGGA+AGG	0.575288	4.4:-79072640	None:intergenic
CTTGCTGCTGATAGGGATGT+GGG	0.575376	4.4:+79073709	MS.gene50392:CDS
AGCGACTCTGACACGCATAT+AGG	0.575736	4.4:-79073040	None:intergenic
GAGACCGTCAAGGTTAAGGT+TGG	0.575959	4.4:-79072644	None:intergenic
AGAATCTAATTCGAACAACT+CGG	0.580030	4.4:-79073205	None:intergenic
ATAGGTTCAAATGTTAGTCA+CGG	0.587320	4.4:+79073742	MS.gene50392:CDS
TGGGAAGGAGGAAACGATGA+GGG	0.589219	4.4:-79072602	None:intergenic
TGGAAATTGTCATACAGCCA+TGG	0.595064	4.4:-79072910	None:intergenic
GAATCTAATTCGAACAACTC+GGG	0.601829	4.4:-79073204	None:intergenic
AGTATGGAAGCCAGGCAAGA+AGG	0.603979	4.4:+79072804	MS.gene50392:CDS
GCAAGGAAACAGAGGAGACT+CGG	0.606376	4.4:-79072574	None:intergenic
GATGACATACAAGAGGTAAG+TGG	0.607658	4.4:+79073634	MS.gene50392:CDS
GGCCGAACTGCAGCTACTGA+AGG	0.610869	4.4:+79073367	MS.gene50392:CDS
CCAAAAGTAGGTCAAGGCAT+AGG	0.611034	4.4:+79072964	MS.gene50392:CDS
AAACGATGAGGGAAACAGCA+AGG	0.612654	4.4:-79072591	None:intergenic
ATCCAACCAAAAGTAGGTCA+AGG	0.617189	4.4:+79072958	MS.gene50392:CDS
CTGGGAAGGAGGAAACGATG+AGG	0.617559	4.4:-79072603	None:intergenic
GTTGGAAGGATCCATGAGAT+CGG	0.617990	4.4:-79072626	None:intergenic
GCTGCACGTATTCTTGATGA+GGG	0.620115	4.4:+79072886	MS.gene50392:CDS
CATACAAGAGGTAAGTGGCT+CGG	0.622041	4.4:+79073639	MS.gene50392:CDS
AAGTGTTTCTCCATTCTGAA+CGG	0.629068	4.4:-79073689	None:intergenic
GCTAGTAGCTAAAGTACCTG+TGG	0.629731	4.4:-79073548	None:intergenic
GGGAAACAGCAAGGAAACAG+AGG	0.631035	4.4:-79072582	None:intergenic
GGTGAAACTGGAAAAGGTAA+CGG	0.632647	4.4:-79072852	None:intergenic
GAAAAGCACTATTTGTGGCA+CGG	0.636628	4.4:-79073101	None:intergenic
ATCTAATGATGACATACAAG+AGG	0.639639	4.4:+79073627	MS.gene50392:CDS
AAGGATCCATGAGATCGGCT+GGG	0.652513	4.4:-79072621	None:intergenic
ATTGAATCTCACGATCAGCA+AGG	0.657379	4.4:-79072753	None:intergenic
ACTGCAGCTACTGAAGGTGT+GGG	0.661239	4.4:+79073373	MS.gene50392:CDS
GGTTAAACCCTCTTCAGACA+AGG	0.669126	4.4:-79072668	None:intergenic
CAGACAAGGTGAGACCGTCA+AGG	0.670109	4.4:-79072654	None:intergenic
TTTGGTTGGATGAATCCGAA+AGG	0.672828	4.4:-79072946	None:intergenic
TGTGCTTGCTGCCCATAATG+CGG	0.690639	4.4:+79073462	MS.gene50392:CDS
GGTTCAAATGTTAGTCACGG+GGG	0.697471	4.4:+79073745	MS.gene50392:CDS
AGGTTCAAATGTTAGTCACG+GGG	0.714709	4.4:+79073744	MS.gene50392:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AACTTTAAATATGAAAAATT+GGG	+	chr4.4:79073132-79073151	MS.gene50392:CDS	10.0%
!!	TAACTTTAAATATGAAAAAT+TGG	+	chr4.4:79073131-79073150	MS.gene50392:CDS	10.0%
!	TATGACAATTTCCATATTCA+CGG	+	chr4.4:79072919-79072938	MS.gene50392:CDS	25.0%
	AATAAGACAGTGTTCAAGAA+TGG	-	chr4.4:79072725-79072744	None:intergenic	30.0%
	AGAATCTAATTCGAACAACT+CGG	-	chr4.4:79073208-79073227	None:intergenic	30.0%
	ATAGGTTCAAATGTTAGTCA+CGG	+	chr4.4:79073742-79073761	MS.gene50392:CDS	30.0%
	ATCTAATGATGACATACAAG+AGG	+	chr4.4:79073627-79073646	MS.gene50392:CDS	30.0%
	ATTCTTGAACACTGTCTTAT+TGG	+	chr4.4:79072724-79072743	MS.gene50392:CDS	30.0%
	TCATTAGATTCACTTGAGAA+TGG	-	chr4.4:79073616-79073635	None:intergenic	30.0%
	TTCGAATTAGATTCTGATGA+TGG	+	chr4.4:79073213-79073232	MS.gene50392:CDS	30.0%
!	GATGTGGGAAATTTTGTAAT+AGG	+	chr4.4:79073724-79073743	MS.gene50392:CDS	30.0%
!!	AGTAAGAAAAGCACTATTTG+TGG	-	chr4.4:79073109-79073128	None:intergenic	30.0%
	AAGTGTTTCTCCATTCTGAA+CGG	-	chr4.4:79073692-79073711	None:intergenic	35.0%
	GAATCTAATTCGAACAACTC+GGG	-	chr4.4:79073207-79073226	None:intergenic	35.0%
	GTGCTAGCTAAAAAAGGTAA+AGG	+	chr4.4:79073484-79073503	MS.gene50392:CDS	35.0%
	TAGGTTCAAATGTTAGTCAC+GGG	+	chr4.4:79073743-79073762	MS.gene50392:CDS	35.0%
	ACTTGAAACCAGTTACGCAA+AGG	+	chr4.4:79073256-79073275	MS.gene50392:intron	40.0%
	AGGTTCAAATGTTAGTCACG+GGG	+	chr4.4:79073744-79073763	MS.gene50392:CDS	40.0%
	ATCCAACCAAAAGTAGGTCA+AGG	+	chr4.4:79072958-79072977	MS.gene50392:CDS	40.0%
	ATCTTGCAACCAACGATTTG+TGG	-	chr4.4:79073295-79073314	None:intergenic	40.0%
	ATTGAATCTCACGATCAGCA+AGG	-	chr4.4:79072756-79072775	None:intergenic	40.0%
	CAAGGCATAGGAATCTTCTT+AGG	+	chr4.4:79072976-79072995	MS.gene50392:CDS	40.0%
	CTTTGTGGTGAAACTGGAAA+AGG	-	chr4.4:79072861-79072880	None:intergenic	40.0%
	GATGACATACAAGAGGTAAG+TGG	+	chr4.4:79073634-79073653	MS.gene50392:CDS	40.0%
	GGATTCATCCAACCAAAAGT+AGG	+	chr4.4:79072952-79072971	MS.gene50392:CDS	40.0%
	GGTGAAACTGGAAAAGGTAA+CGG	-	chr4.4:79072855-79072874	None:intergenic	40.0%
	GTTACAAACCTTTGCGTAAC+TGG	-	chr4.4:79073267-79073286	None:intergenic	40.0%
	GTTTGATGCAACATAGAAGC+AGG	-	chr4.4:79073324-79073343	None:intergenic	40.0%
	TGCAACATAGAAGCAGGAAT+AGG	-	chr4.4:79073318-79073337	None:intergenic	40.0%
	TGCTTCCAAAATACATCTGC+TGG	+	chr4.4:79073516-79073535	MS.gene50392:CDS	40.0%
	TGGAAATTGTCATACAGCCA+TGG	-	chr4.4:79072913-79072932	None:intergenic	40.0%
	TTTAGCTAGCACCGCATTAT+GGG	-	chr4.4:79073476-79073495	None:intergenic	40.0%
	TTTGGTTGGATGAATCCGAA+AGG	-	chr4.4:79072949-79072968	None:intergenic	40.0%
!	AATGCGGTGCTAGCTAAAAA+AGG	+	chr4.4:79073478-79073497	MS.gene50392:CDS	40.0%
!	AATTGGGTGTGTTTTGTCAC+AGG	+	chr4.4:79073148-79073167	MS.gene50392:CDS	40.0%
!	AGAAACACTTGCTGCTGATA+GGG	+	chr4.4:79073702-79073721	MS.gene50392:CDS	40.0%
!	AGAAGCCAGCAGATGTATTT+TGG	-	chr4.4:79073524-79073543	None:intergenic	40.0%
!	AGACATACGCTTGCTGAAAT+AGG	-	chr4.4:79072782-79072801	None:intergenic	40.0%
!	AGGTTCGGTTTCAAGGTTAA+GGG	-	chr4.4:79072692-79072711	None:intergenic	40.0%
!!	GAAAAGCACTATTTGTGGCA+CGG	-	chr4.4:79073104-79073123	None:intergenic	40.0%
!!	TTTTAGCTAGCACCGCATTA+TGG	-	chr4.4:79073477-79073496	None:intergenic	40.0%
	AAACGATGAGGGAAACAGCA+AGG	-	chr4.4:79072594-79072613	None:intergenic	45.0%
	AATGGCAGCAGTATCAGGTT+CGG	-	chr4.4:79072707-79072726	None:intergenic	45.0%
	CATACAAGAGGTAAGTGGCT+CGG	+	chr4.4:79073639-79073658	MS.gene50392:CDS	45.0%
	CATATTCACGGTCTGCCTTT+CGG	+	chr4.4:79072931-79072950	MS.gene50392:CDS	45.0%
	CCAAAAGTAGGTCAAGGCAT+AGG	+	chr4.4:79072964-79072983	MS.gene50392:CDS	45.0%
	CCTTCCAACCTTAACCTTGA+CGG	+	chr4.4:79072640-79072659	MS.gene50392:CDS	45.0%
	CTGTCCCACTGAAAAAAGAG+TGG	+	chr4.4:79073073-79073092	MS.gene50392:CDS	45.0%
	GCTGCACGTATTCTTGATGA+GGG	+	chr4.4:79072886-79072905	MS.gene50392:CDS	45.0%
	GGTTAAACCCTCTTCAGACA+AGG	-	chr4.4:79072671-79072690	None:intergenic	45.0%
	GGTTCAAATGTTAGTCACGG+GGG	+	chr4.4:79073745-79073764	MS.gene50392:CDS	45.0%
	GTATTCTTGATGAGGGTCCA+TGG	+	chr4.4:79072893-79072912	MS.gene50392:CDS	45.0%
	GTTCAAATGTTAGTCACGGG+GGG	+	chr4.4:79073746-79073765	MS.gene50392:CDS	45.0%
	GTTGGAAGGATCCATGAGAT+CGG	-	chr4.4:79072629-79072648	None:intergenic	45.0%
	TCAAGAATGGCAGCAGTATC+AGG	-	chr4.4:79072712-79072731	None:intergenic	45.0%
	TGCTGCACGTATTCTTGATG+AGG	+	chr4.4:79072885-79072904	MS.gene50392:CDS	45.0%
	TTCAAATGTTAGTCACGGGG+GGG	+	chr4.4:79073747-79073766	MS.gene50392:CDS	45.0%
!	CAGGTTCGGTTTCAAGGTTA+AGG	-	chr4.4:79072693-79072712	None:intergenic	45.0%
!	CAGTATCAGGTTCGGTTTCA+AGG	-	chr4.4:79072699-79072718	None:intergenic	45.0%
!	CCTATGCCTTGACCTACTTT+TGG	-	chr4.4:79072967-79072986	None:intergenic	45.0%
!	GAGAAACACTTGCTGCTGAT+AGG	+	chr4.4:79073701-79073720	MS.gene50392:CDS	45.0%
!	GCTAGTAGCTAAAGTACCTG+TGG	-	chr4.4:79073551-79073570	None:intergenic	45.0%
!	GTAACGGTCAGACACTGATT+TGG	-	chr4.4:79072839-79072858	None:intergenic	45.0%
!	TGCGTAACTGGTTTCAAGTC+AGG	-	chr4.4:79073255-79073274	None:intergenic	45.0%
!	TGTCACAGGTGTGGATTGAT+TGG	+	chr4.4:79073162-79073181	MS.gene50392:CDS	45.0%
!!	CATCGACTTTGTGGTGAAAC+TGG	-	chr4.4:79072867-79072886	None:intergenic	45.0%
!!	TGCCTTGACCTACTTTTGGT+TGG	-	chr4.4:79072963-79072982	None:intergenic	45.0%
!!!	ACACGCCACTCTTTTTTCAG+TGG	-	chr4.4:79073081-79073100	None:intergenic	45.0%
!!!	ACCAAGCAAGCTGCTTTTGT+TGG	+	chr4.4:79073346-79073365	MS.gene50392:intron	45.0%
!!!	CACGCCACTCTTTTTTCAGT+GGG	-	chr4.4:79073080-79073099	None:intergenic	45.0%
	AACTGCAGCTACTGAAGGTG+TGG	+	chr4.4:79073372-79073391	MS.gene50392:CDS	50.0%
	AAGGATCCATGAGATCGGCT+GGG	-	chr4.4:79072624-79072643	None:intergenic	50.0%
	AGCGACTCTGACACGCATAT+AGG	-	chr4.4:79073043-79073062	None:intergenic	50.0%
	AGGTGAGACCGTCAAGGTTA+AGG	-	chr4.4:79072651-79072670	None:intergenic	50.0%
	AGTATGGAAGCCAGGCAAGA+AGG	+	chr4.4:79072804-79072823	MS.gene50392:CDS	50.0%
	ATGTCTCGAGTATGGAAGCC+AGG	+	chr4.4:79072796-79072815	MS.gene50392:CDS	50.0%
	CAACCAACGATTTGTGGCAG+CGG	-	chr4.4:79073289-79073308	None:intergenic	50.0%
	CACCTTCAGTAGCTGCAGTT+CGG	-	chr4.4:79073372-79073391	None:intergenic	50.0%
	CCGTCAAGGTTAAGGTTGGA+AGG	-	chr4.4:79072643-79072662	None:intergenic	50.0%
	CGAAAGGCAGACCGTGAATA+TGG	-	chr4.4:79072933-79072952	None:intergenic	50.0%
	CTTGCTGCTGATAGGGATGT+GGG	+	chr4.4:79073709-79073728	MS.gene50392:CDS	50.0%
	GAGACCGTCAAGGTTAAGGT+TGG	-	chr4.4:79072647-79072666	None:intergenic	50.0%
	GCAAGCGTATGTCTCGAGTA+TGG	+	chr4.4:79072788-79072807	MS.gene50392:CDS	50.0%
	GCAAGGAAACAGAGGAGACT+CGG	-	chr4.4:79072577-79072596	None:intergenic	50.0%
	GGGAAACAGCAAGGAAACAG+AGG	-	chr4.4:79072585-79072604	None:intergenic	50.0%
	GGTCTCACCTTGTCTGAAGA+GGG	+	chr4.4:79072661-79072680	MS.gene50392:CDS	50.0%
	GTGATCGTAACCTTCTTGCC+TGG	-	chr4.4:79072817-79072836	None:intergenic	50.0%
	TAACCGCTGCCACAAATCGT+TGG	+	chr4.4:79073283-79073302	MS.gene50392:intron	50.0%
	TCAAATGTTAGTCACGGGGG+GGG	+	chr4.4:79073748-79073767	MS.gene50392:CDS	50.0%
	TCAAGTCAGGTCCCCAATTG+CGG	-	chr4.4:79073242-79073261	None:intergenic	50.0%
	TCACTTGAGAATGGAGCAGC+AGG	-	chr4.4:79073607-79073626	None:intergenic	50.0%
	TGGGAAGGAGGAAACGATGA+GGG	-	chr4.4:79072605-79072624	None:intergenic	50.0%
!	ACTGCAGCTACTGAAGGTGT+GGG	+	chr4.4:79073373-79073392	MS.gene50392:CDS	50.0%
!	ACTTGCTGCTGATAGGGATG+TGG	+	chr4.4:79073708-79073727	MS.gene50392:CDS	50.0%
!	GAAGAAAAGAAAGCGTGCGC+TGG	+	chr4.4:79073666-79073685	MS.gene50392:CDS	50.0%
!	GCCAACAAAAGCAGCTTGCT+TGG	-	chr4.4:79073350-79073369	None:intergenic	50.0%
!	GGTGTGTTTTGTCACAGGTG+TGG	+	chr4.4:79073153-79073172	MS.gene50392:CDS	50.0%
!	TGTGCTTGCTGCCCATAATG+CGG	+	chr4.4:79073462-79073481	MS.gene50392:CDS	50.0%
!!	TACGTGCAGCATCGACTTTG+TGG	-	chr4.4:79072876-79072895	None:intergenic	50.0%
!!	TGATGATGGCGTCCGCAATT+GGG	+	chr4.4:79073227-79073246	MS.gene50392:CDS	50.0%
	AAATGTTAGTCACGGGGGGG+GGG	+	chr4.4:79073750-79073769	MS.gene50392:CDS	55.0%
	ATCCATGAGATCGGCTGGGA+AGG	-	chr4.4:79072620-79072639	None:intergenic	55.0%
	CAAATGTTAGTCACGGGGGG+GGG	+	chr4.4:79073749-79073768	MS.gene50392:CDS	55.0%
	CAGACAAGGTGAGACCGTCA+AGG	-	chr4.4:79072657-79072676	None:intergenic	55.0%
	CCAAGCACCAAAGGACGACT+TGG	-	chr4.4:79073580-79073599	None:intergenic	55.0%
	CCTGCTTCCAAGTCGTCCTT+TGG	+	chr4.4:79073570-79073589	MS.gene50392:CDS	55.0%
	CGGTCTCACCTTGTCTGAAG+AGG	+	chr4.4:79072660-79072679	MS.gene50392:CDS	55.0%
	CTGGGAAGGAGGAAACGATG+AGG	-	chr4.4:79072606-79072625	None:intergenic	55.0%
	GAAGGATCCATGAGATCGGC+TGG	-	chr4.4:79072625-79072644	None:intergenic	55.0%
	GCAGGTAAGCCAAGCACCAA+AGG	-	chr4.4:79073589-79073608	None:intergenic	55.0%
!	CCAAAGGACGACTTGGAAGC+AGG	-	chr4.4:79073573-79073592	None:intergenic	55.0%
!	GATGATGGCGTCCGCAATTG+GGG	+	chr4.4:79073228-79073247	MS.gene50392:CDS	55.0%
!!	CCAAGTCGTCCTTTGGTGCT+TGG	+	chr4.4:79073577-79073596	MS.gene50392:CDS	55.0%
!!	CTGATGATGGCGTCCGCAAT+TGG	+	chr4.4:79073226-79073245	MS.gene50392:CDS	55.0%
!!	CTGCTGGCTTCTTCTTCCAC+AGG	+	chr4.4:79073532-79073551	MS.gene50392:CDS	55.0%
	AATGTTAGTCACGGGGGGGG+GGG	+	chr4.4:79073751-79073770	MS.gene50392:CDS	60.0%
	CATGAGATCGGCTGGGAAGG+AGG	-	chr4.4:79072617-79072636	None:intergenic	60.0%
	CTCCTTCCCAGCCGATCTCA+TGG	+	chr4.4:79072615-79072634	MS.gene50392:CDS	60.0%
	GGCCGAACTGCAGCTACTGA+AGG	+	chr4.4:79073367-79073386	MS.gene50392:CDS	60.0%
	ATGTTAGTCACGGGGGGGGG+GGG	+	chr4.4:79073752-79073771	MS.gene50392:CDS	65.0%
!	CGTGCGCTGGCCGTTCAGAA+TGG	+	chr4.4:79073679-79073698	MS.gene50392:CDS	65.0%
	TGTTAGTCACGGGGGGGGGG+GGG	+	chr4.4:79073753-79073772	MS.gene50392:CDS	70.0%
	GTTAGTCACGGGGGGGGGGG+GGG	+	chr4.4:79073754-79073773	MS.gene50392:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr4.4	gene	79072553	79073783	79072553	ID=MS.gene50392
chr4.4	mRNA	79072553	79073783	79072553	ID=MS.gene50392.t1;Parent=MS.gene50392
chr4.4	exon	79072553	79073266	79072553	ID=MS.gene50392.t1.exon1;Parent=MS.gene50392.t1
chr4.4	CDS	79072553	79073266	79072553	ID=cds.MS.gene50392.t1;Parent=MS.gene50392.t1
chr4.4	exon	79073352	79073783	79073352	ID=MS.gene50392.t1.exon2;Parent=MS.gene50392.t1
chr4.4	CDS	79073352	79073783	79073352	ID=cds.MS.gene50392.t1;Parent=MS.gene50392.t1

Gene Sequence

>MS.gene50392

ATGCGTTTTACTGTTTCTAAACCGAGTCTCCTCTGTTTCCTTGCTGTTTCCCTCATCGTTTCCTCCTTCCCAGCCGATCTCATGGATCCTTCCAACCTTAACCTTGACGGTCTCACCTTGTCTGAAGAGGGTTTAACCCTTAACCTTGAAACCGAACCTGATACTGCTGCCATTCTTGAACACTGTCTTATTGGTCGAGTCCTTGCTGATCGTGAGATTCAATTTGCCTATTTCAGCAAGCGTATGTCTCGAGTATGGAAGCCAGGCAAGAAGGTTACGATCACCAAATCAGTGTCTGACCGTTACCTTTTCCAGTTTCACCACAAAGTCGATGCTGCACGTATTCTTGATGAGGGTCCATGGCTGTATGACAATTTCCATATTCACGGTCTGCCTTTCGGATTCATCCAACCAAAAGTAGGTCAAGGCATAGGAATCTTCTTAGGCACTTTCAAAGCATATGATAATCGCAACTCAATTCATAGTTCCTATATGCGTGTCAGAGTCGCTATTGATGTCACTGTCCCACTGAAAAAAGAGTGGCGTGTCCGTGCCACAAATAGTGCTTTTCTTACTGTTAACTTTAAATATGAAAAATTGGGTGTGTTTTGTCACAGGTGTGGATTGATTGGTCATACTGACAAAGTGTGTCCCGAGTTGTTCGAATTAGATTCTGATGATGGCGTCCGCAATTGGGGACCTGACTTGAAACCAGTTACGCAAAGGTTTGTAACCGCTGCCACAAATCGTTGGTTGCAAGATCCTATTCCTGCTTCTATGTTGCATCAAACTCACCAAGCAAGCTGCTTTTGTTGGCCGAACTGCAGCTACTGAAGGTGTGGGAAATTATGCAACTTTCAATGATCGTAAGCTCACTCTTCAGACGCAACTTACTGCTATGCAACAAGATGTGCTTGCTGCCCATAATGCGGTGCTAGCTAAAAAAGGTAAAGGTGCAAGTTCTGCTTCCAAAATACATCTGCTGGCTTCTTCTTCCACAGGTACTTTAGCTACTAGCCTGCTTCCAAGTCGTCCTTTGGTGCTTGGCTTACCTGCTGCTCCATTCTCAAGTGAATCTAATGATGACATACAAGAGGTAAGTGGCTCGGATTTGAAGAAAAGAAAGCGTGCGCTGGCCGTTCAGAATGGAGAAACACTTGCTGCTGATAGGGATGTGGGAAATTTTGTAATAGGTTCAAATGTTAGTCACGGGGGGGGGGGGGGATATTGA

Protein sequence

>MS.gene50392.t1

MRFTVSKPSLLCFLAVSLIVSSFPADLMDPSNLNLDGLTLSEEGLTLNLETEPDTAAILEHCLIGRVLADREIQFAYFSKRMSRVWKPGKKVTITKSVSDRYLFQFHHKVDAARILDEGPWLYDNFHIHGLPFGFIQPKVGQGIGIFLGTFKAYDNRNSIHSSYMRVRVAIDVTVPLKKEWRVRATNSAFLTVNFKYEKLGVFCHRCGLIGHTDKVCPELFELDSDDGVRNWGPDLKPQAAFVGRTAATEGVGNYATFNDRKLTLQTQLTAMQQDVLAAHNAVLAKKGKGASSASKIHLLASSSTGTLATSLLPSRPLVLGLPAAPFSSESNDDIQEVSGSDLKKRKRALAVQNGETLAADRDVGNFVIGSNVSHGGGGGY