AlfalfaGEDB | MS.gene59460

M. sativa cultivar XinJiangDaYe / MS.gene59460

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59460.t1	XP_024629915.1	84.7	320	49	0	1	320	1	320	8.50E-155	556.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59460.t1	A0A392M033	68.1	361	115	0	1	361	1	361	2.1e-139	505.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene073582	MS.gene59460	PPI
MS.gene058889	MS.gene59460	PPI
MS.gene98616	MS.gene59460	PPI
MS.gene40828	MS.gene59460	PPI
MS.gene53904	MS.gene59460	PPI
MS.gene08708	MS.gene59460	PPI
MS.gene09517	MS.gene59460	PPI
MS.gene69586	MS.gene59460	PPI
MS.gene042027	MS.gene59460	PPI
MS.gene69490	MS.gene59460	PPI
MS.gene043140	MS.gene59460	PPI
MS.gene015248	MS.gene59460	PPI
MS.gene84790	MS.gene59460	PPI
MS.gene80362	MS.gene59460	PPI
MS.gene44083	MS.gene59460	PPI
MS.gene37537	MS.gene59460	PPI
MS.gene013193	MS.gene59460	PPI
MS.gene04970	MS.gene59460	PPI
MS.gene08106	MS.gene59460	PPI
MS.gene24278	MS.gene59460	PPI
MS.gene59460	MS.gene022929	PPI
MS.gene025158	MS.gene59460	PPI
MS.gene027994	MS.gene59460	PPI
MS.gene068629	MS.gene59460	PPI
MS.gene019001	MS.gene59460	PPI
MS.gene96163	MS.gene59460	PPI
MS.gene08713	MS.gene59460	PPI
MS.gene23521	MS.gene59460	PPI
MS.gene63150	MS.gene59460	PPI
MS.gene59460	MS.gene048342	PPI
MS.gene54051	MS.gene59460	PPI
MS.gene98127	MS.gene59460	PPI
MS.gene03149	MS.gene59460	PPI
MS.gene59460	MS.gene21066	PPI
MS.gene87529	MS.gene59460	PPI
MS.gene024405	MS.gene59460	PPI
MS.gene012748	MS.gene59460	PPI
MS.gene04134	MS.gene59460	PPI
MS.gene83979	MS.gene59460	PPI
MS.gene062067	MS.gene59460	PPI
MS.gene92517	MS.gene59460	PPI
MS.gene057934	MS.gene59460	PPI
MS.gene57044	MS.gene59460	PPI
MS.gene054508	MS.gene59460	PPI
MS.gene89815	MS.gene59460	PPI
MS.gene59460	MS.gene008658	PPI
MS.gene028714	MS.gene59460	PPI
MS.gene049610	MS.gene59460	PPI
MS.gene017586	MS.gene59460	PPI
MS.gene038676	MS.gene59460	PPI
MS.gene060718	MS.gene59460	PPI
MS.gene71637	MS.gene59460	PPI
MS.gene042651	MS.gene59460	PPI
MS.gene05576	MS.gene59460	PPI
MS.gene52349	MS.gene59460	PPI
MS.gene98467	MS.gene59460	PPI
MS.gene46353	MS.gene59460	PPI
MS.gene42010	MS.gene59460	PPI
MS.gene037049	MS.gene59460	PPI
MS.gene034643	MS.gene59460	PPI
MS.gene037634	MS.gene59460	PPI
MS.gene000379	MS.gene59460	PPI
MS.gene048342	MS.gene59460	PPI
MS.gene64130	MS.gene59460	PPI
MS.gene24070	MS.gene59460	PPI
MS.gene60266	MS.gene59460	PPI
MS.gene016719	MS.gene59460	PPI
MS.gene071784	MS.gene59460	PPI
MS.gene59460	MS.gene74639	PPI
MS.gene59460	MS.gene071133	PPI
MS.gene061579	MS.gene59460	PPI
MS.gene039692	MS.gene59460	PPI
MS.gene76420	MS.gene59460	PPI
MS.gene071320	MS.gene59460	PPI
MS.gene022929	MS.gene59460	PPI
MS.gene81712	MS.gene59460	PPI
MS.gene026680	MS.gene59460	PPI
MS.gene26433	MS.gene59460	PPI
MS.gene34463	MS.gene59460	PPI
MS.gene59460	MS.gene37955	PPI
MS.gene22730	MS.gene59460	PPI
MS.gene59460	MS.gene84790	PPI
MS.gene042559	MS.gene59460	PPI
MS.gene22200	MS.gene59460	PPI
MS.gene071133	MS.gene59460	PPI
MS.gene055546	MS.gene59460	PPI
MS.gene26038	MS.gene59460	PPI
MS.gene08606	MS.gene59460	PPI
MS.gene85308	MS.gene59460	PPI
MS.gene49057	MS.gene59460	PPI
MS.gene012889	MS.gene59460	PPI
MS.gene031967	MS.gene59460	PPI
MS.gene053373	MS.gene59460	PPI
MS.gene34812	MS.gene59460	PPI
MS.gene02896	MS.gene59460	PPI
MS.gene063214	MS.gene59460	PPI
MS.gene55682	MS.gene59460	PPI
MS.gene59460	MS.gene027994	PPI
MS.gene51799	MS.gene59460	PPI
MS.gene59460	MS.gene007022	PPI
MS.gene58419	MS.gene59460	PPI
MS.gene067340	MS.gene59460	PPI
MS.gene65623	MS.gene59460	PPI
MS.gene65474	MS.gene59460	PPI
MS.gene019177	MS.gene59460	PPI
MS.gene69495	MS.gene59460	PPI
MS.gene049512	MS.gene59460	PPI
MS.gene29406	MS.gene59460	PPI
MS.gene038623	MS.gene59460	PPI
MS.gene86275	MS.gene59460	PPI
MS.gene05357	MS.gene59460	PPI
MS.gene59460	MS.gene61651	PPI
MS.gene032311	MS.gene59460	PPI
MS.gene004417	MS.gene59460	PPI
MS.gene86849	MS.gene59460	PPI
MS.gene50392	MS.gene59460	PPI
MS.gene59460	MS.gene88393	PPI
MS.gene37955	MS.gene59460	PPI
MS.gene072852	MS.gene59460	PPI
MS.gene41393	MS.gene59460	PPI
MS.gene39956	MS.gene59460	PPI
MS.gene021968	MS.gene59460	PPI
MS.gene98756	MS.gene59460	PPI
MS.gene09589	MS.gene59460	PPI
MS.gene44144	MS.gene59460	PPI
MS.gene43393	MS.gene59460	PPI
MS.gene057828	MS.gene59460	PPI
MS.gene59460	MS.gene015093	PPI
MS.gene34197	MS.gene59460	PPI
MS.gene59460	MS.gene000379	PPI
MS.gene044729	MS.gene59460	PPI
MS.gene66650	MS.gene59460	PPI
MS.gene005120	MS.gene59460	PPI
MS.gene86868	MS.gene59460	PPI
MS.gene59460	MS.gene08606	PPI
MS.gene048072	MS.gene59460	PPI
MS.gene060386	MS.gene59460	PPI
MS.gene59460	MS.gene000640	PPI
MS.gene71588	MS.gene59460	PPI
MS.gene04749	MS.gene59460	PPI
MS.gene59460	MS.gene09517	PPI
MS.gene05436	MS.gene59460	PPI
MS.gene059736	MS.gene59460	PPI
MS.gene70932	MS.gene59460	PPI
MS.gene027169	MS.gene59460	PPI
MS.gene59460	MS.gene62249	PPI
MS.gene62249	MS.gene59460	PPI
MS.gene81194	MS.gene59460	PPI
MS.gene025675	MS.gene59460	PPI
MS.gene42903	MS.gene59460	PPI
MS.gene021965	MS.gene59460	PPI
MS.gene055683	MS.gene59460	PPI
MS.gene042614	MS.gene59460	PPI
MS.gene41643	MS.gene59460	PPI
MS.gene26844	MS.gene59460	PPI
MS.gene021555	MS.gene59460	PPI
MS.gene060773	MS.gene59460	PPI
MS.gene048747	MS.gene59460	PPI
MS.gene021647	MS.gene59460	PPI
MS.gene69584	MS.gene59460	PPI
MS.gene019165	MS.gene59460	PPI
MS.gene048706	MS.gene59460	PPI
MS.gene59460	MS.gene60390	PPI
MS.gene009121	MS.gene59460	PPI
MS.gene94654	MS.gene59460	PPI
MS.gene024841	MS.gene59460	PPI
MS.gene073573	MS.gene59460	PPI
MS.gene51804	MS.gene59460	PPI
MS.gene87845	MS.gene59460	PPI
MS.gene36069	MS.gene59460	PPI
MS.gene97410	MS.gene59460	PPI
MS.gene83772	MS.gene59460	PPI
MS.gene32415	MS.gene59460	PPI
MS.gene009213	MS.gene59460	PPI
MS.gene060788	MS.gene59460	PPI
MS.gene035626	MS.gene59460	PPI
MS.gene008927	MS.gene59460	PPI
MS.gene045614	MS.gene59460	PPI
MS.gene049561	MS.gene59460	PPI
MS.gene009781	MS.gene59460	PPI
MS.gene63762	MS.gene59460	PPI
MS.gene59460	MS.gene025158	PPI
MS.gene27329	MS.gene59460	PPI
MS.gene71675	MS.gene59460	PPI
MS.gene30208	MS.gene59460	PPI
MS.gene028716	MS.gene59460	PPI
MS.gene21066	MS.gene59460	PPI
MS.gene06164	MS.gene59460	PPI
MS.gene013676	MS.gene59460	PPI
MS.gene55209	MS.gene59460	PPI
MS.gene64178	MS.gene59460	PPI
MS.gene030090	MS.gene59460	PPI
MS.gene95561	MS.gene59460	PPI
MS.gene96174	MS.gene59460	PPI
MS.gene018196	MS.gene59460	PPI
MS.gene065627	MS.gene59460	PPI
MS.gene57019	MS.gene59460	PPI
MS.gene59460	MS.gene025987	PPI
MS.gene010890	MS.gene59460	PPI
MS.gene018745	MS.gene59460	PPI
MS.gene65546	MS.gene59460	PPI
MS.gene09023	MS.gene59460	PPI
MS.gene59460	MS.gene043140	PPI
MS.gene000506	MS.gene59460	PPI
MS.gene74639	MS.gene59460	PPI
MS.gene59460	MS.gene03149	PPI
MS.gene032299	MS.gene59460	PPI
MS.gene03936	MS.gene59460	PPI
MS.gene038098	MS.gene59460	PPI
MS.gene60059	MS.gene59460	PPI
MS.gene062511	MS.gene59460	PPI
MS.gene22111	MS.gene59460	PPI
MS.gene28718	MS.gene59460	PPI
MS.gene055560	MS.gene59460	PPI
MS.gene008275	MS.gene59460	PPI
MS.gene59460	MS.gene24278	PPI
MS.gene29321	MS.gene59460	PPI
MS.gene34618	MS.gene59460	PPI
MS.gene038757	MS.gene59460	PPI
MS.gene064966	MS.gene59460	PPI
MS.gene069624	MS.gene59460	PPI
MS.gene016632	MS.gene59460	PPI
MS.gene002590	MS.gene59460	PPI
MS.gene025987	MS.gene59460	PPI
MS.gene000640	MS.gene59460	PPI
MS.gene43924	MS.gene59460	PPI
MS.gene046089	MS.gene59460	PPI
MS.gene047954	MS.gene59460	PPI
MS.gene35175	MS.gene59460	PPI
MS.gene77990	MS.gene59460	PPI
MS.gene036423	MS.gene59460	PPI
MS.gene069786	MS.gene59460	PPI
MS.gene054652	MS.gene59460	PPI
MS.gene013644	MS.gene59460	PPI
MS.gene013890	MS.gene59460	PPI
MS.gene59460	MS.gene44144	PPI
MS.gene72520	MS.gene59460	PPI
MS.gene61651	MS.gene59460	PPI
MS.gene66651	MS.gene59460	PPI
MS.gene96109	MS.gene59460	PPI
MS.gene35202	MS.gene59460	PPI
MS.gene051449	MS.gene59460	PPI
MS.gene015093	MS.gene59460	PPI
MS.gene007022	MS.gene59460	PPI
MS.gene61499	MS.gene59460	PPI
MS.gene63743	MS.gene59460	PPI
MS.gene48477	MS.gene59460	PPI
MS.gene045875	MS.gene59460	PPI
MS.gene038394	MS.gene59460	PPI
MS.gene30136	MS.gene59460	PPI
MS.gene95960	MS.gene59460	PPI
MS.gene008861	MS.gene59460	PPI
MS.gene61452	MS.gene59460	PPI
MS.gene73261	MS.gene59460	PPI
MS.gene024297	MS.gene59460	PPI
MS.gene016102	MS.gene59460	PPI
MS.gene46941	MS.gene59460	PPI
MS.gene96541	MS.gene59460	PPI
MS.gene45083	MS.gene59460	PPI
MS.gene000634	MS.gene59460	PPI
MS.gene85295	MS.gene59460	PPI
MS.gene60390	MS.gene59460	PPI
MS.gene47792	MS.gene59460	PPI
MS.gene46948	MS.gene59460	PPI
MS.gene98471	MS.gene59460	PPI
MS.gene025873	MS.gene59460	PPI
MS.gene012019	MS.gene59460	PPI
MS.gene025401	MS.gene59460	PPI
MS.gene05358	MS.gene59460	PPI
MS.gene59460	MS.gene018474	PPI
MS.gene59460	MS.gene86868	PPI
MS.gene93396	MS.gene59460	PPI
MS.gene074257	MS.gene59460	PPI
MS.gene05231	MS.gene59460	PPI
MS.gene010656	MS.gene59460	PPI
MS.gene000629	MS.gene59460	PPI
MS.gene059269	MS.gene59460	PPI
MS.gene40469	MS.gene59460	PPI
MS.gene041264	MS.gene59460	PPI
MS.gene41979	MS.gene59460	PPI
MS.gene024522	MS.gene59460	PPI
MS.gene05319	MS.gene59460	PPI
MS.gene88393	MS.gene59460	PPI
MS.gene56991	MS.gene59460	PPI
MS.gene050679	MS.gene59460	PPI
MS.gene85538	MS.gene59460	PPI
MS.gene20246	MS.gene59460	PPI
MS.gene055223	MS.gene59460	PPI
MS.gene28545	MS.gene59460	PPI
MS.gene010290	MS.gene59460	PPI
MS.gene053747	MS.gene59460	PPI
MS.gene59460	MS.gene61499	PPI
MS.gene65471	MS.gene59460	PPI
MS.gene061850	MS.gene59460	PPI
MS.gene048088	MS.gene59460	PPI
MS.gene008658	MS.gene59460	PPI
MS.gene018474	MS.gene59460	PPI
MS.gene003264	MS.gene59460	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59460.t1	MTR_0010s0370	35.374	147	94	1	35	180	34	180	9.09e-25	100
MS.gene59460.t1	MTR_0010s0350	35.374	147	94	1	35	180	34	180	9.09e-25	100
MS.gene59460.t1	MTR_6g033215	22.543	173	128	3	29	197	123	293	6.88e-11	62.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene59460.t1	AT3G31430	25.434	173	119	4	46	214	145	311	8.33e-13	68.9
MS.gene59460.t1	AT2G01050	23.415	205	149	5	12	214	58	256	1.21e-12	69.3

Find 100 sgRNAs with CRISPR-Local

Find 120 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TGCTCAACATTTGGTTCTTT+TGG	0.143736	5.4:+13516655	None:intergenic
ACTTTCAGGGTAAGGATATC+TGG	0.260595	5.4:-13516975	MS.gene59460:CDS
AGGCTCCAAACTGCACTTTC+AGG	0.268954	5.4:-13516989	MS.gene59460:CDS
CCATAGTTGTACCTGAATTC+TGG	0.284462	5.4:+13516701	None:intergenic
ATTAGCAAGGGCCAGAATTC+AGG	0.302102	5.4:-13516712	MS.gene59460:CDS
GAAGGCGTGGCTGAATCTTT+GGG	0.308939	5.4:-13516547	MS.gene59460:CDS
AGAAGGCGTGGCTGAATCTT+TGG	0.310007	5.4:-13516548	MS.gene59460:CDS
GAAGAGAAAGATCAAAATTA+TGG	0.317312	5.4:-13516835	MS.gene59460:CDS
CGATGAACAGATCCTCCTTC+TGG	0.338653	5.4:-13517176	MS.gene59460:CDS
TTGTTATTCTGCTCAACATT+TGG	0.338917	5.4:+13516646	None:intergenic
CTAGTCCTCAATCGCATCTT+CGG	0.343864	5.4:-13517222	MS.gene59460:CDS
CGAAGATGCGATTGAGGACT+AGG	0.347461	5.4:+13517223	None:intergenic
TTGGATCATGGTTTGTTTGA+AGG	0.354009	5.4:+13517374	None:intergenic
ATCATCAGCAATTGGTCTCT+TGG	0.357636	5.4:+13517503	None:intergenic
CAGCTTCACTGAAATCTTCC+TGG	0.360386	5.4:+13516857	None:intergenic
GGCAGCGAAAGTGGGAGATT+TGG	0.363229	5.4:-13516593	MS.gene59460:CDS
CCACGCCTTCTACATCTTCC+TGG	0.365452	5.4:+13516560	None:intergenic
CTTACCCTGAAAGTGCAGTT+TGG	0.386156	5.4:+13516984	None:intergenic
CTTCGGGAACGAGCAACCTT+TGG	0.392225	5.4:-13517205	MS.gene59460:CDS
CCTTGGAGACTTCGAAGAAA+TGG	0.395204	5.4:-13517088	MS.gene59460:CDS
CGCTCTCCAAGCTTGGATCA+TGG	0.398027	5.4:+13517362	None:intergenic
TCTACTCTCCCAACACATAA+TGG	0.399314	5.4:-13516511	MS.gene59460:CDS
TTGAAGGCTCTAGTGTTGTA+TGG	0.402845	5.4:+13517390	None:intergenic
TAGTCCTCAATCGCATCTTC+GGG	0.417144	5.4:-13517221	MS.gene59460:CDS
CTCTGTTGAGGCAGCGAAAG+TGG	0.425490	5.4:-13516602	MS.gene59460:CDS
TTCGAAGCAAGTAAAGAGTT+AGG	0.425950	5.4:-13516775	MS.gene59460:CDS
CCAGAATTCAGGTACAACTA+TGG	0.431169	5.4:-13516701	MS.gene59460:CDS
TTCAAATCTTGGATTTCGAT+CGG	0.436489	5.4:+13517333	None:intergenic
TCTCAGAATCAGAGCCTCTC+TGG	0.442752	5.4:-13517034	MS.gene59460:CDS
TATGAAGGAGATAAATAGAA+TGG	0.445307	5.4:-13517064	MS.gene59460:CDS
GGTTCCTCGCTCTCCAAGCT+TGG	0.451963	5.4:+13517355	None:intergenic
TCTTTGATGTGAACTTGAAA+AGG	0.452052	5.4:+13517298	None:intergenic
CACTGAAATCTTCCTGGTCA+TGG	0.456647	5.4:+13516863	None:intergenic
ATCAAAGAGAGAAGCAGATC+TGG	0.460640	5.4:-13517283	MS.gene59460:CDS
ATGAAGGAGATAAATAGAAT+GGG	0.465394	5.4:-13517063	MS.gene59460:CDS
GAAAGGGGAGAAAGTGGGTC+AGG	0.467120	5.4:-13516465	MS.gene59460:CDS
GCCTCATCATCATCAGCAAT+TGG	0.468070	5.4:+13517495	None:intergenic
TCAAATCTTGGATTTCGATC+GGG	0.471138	5.4:+13517334	None:intergenic
GAGAAAGTGGGTCAGGCAGA+AGG	0.473399	5.4:-13516458	MS.gene59460:CDS
GCAGATCTGGTATGCAAGAA+TGG	0.480725	5.4:-13517270	MS.gene59460:CDS
CTTCGAAGAAATGGATATGA+AGG	0.487737	5.4:-13517079	MS.gene59460:CDS
ACGAAGGAAAGGGGAGAAAG+TGG	0.491881	5.4:-13516471	MS.gene59460:CDS
TCTACATCTTCCTGGACGAT+TGG	0.492705	5.4:+13516568	None:intergenic
GGCTCCAAACTGCACTTTCA+GGG	0.494244	5.4:-13516988	MS.gene59460:CDS
AGTGGCGAAGCTCTGAGCCA+TGG	0.495578	5.4:+13516811	None:intergenic
GCTCAGCACCATTATGTGTT+GGG	0.501077	5.4:+13516503	None:intergenic
AAGGCGTGGCTGAATCTTTG+GGG	0.501122	5.4:-13516546	MS.gene59460:CDS
TGAAGGCTCTAGTGTTGTAT+GGG	0.501139	5.4:+13517391	None:intergenic
GTACTCTCGTCGGAAAACTC+TGG	0.502284	5.4:-13517422	MS.gene59460:CDS
CAAACTGCACTTTCAGGGTA+AGG	0.507533	5.4:-13516983	MS.gene59460:CDS
TAACTCTTTACTTGCTTCGA+AGG	0.507906	5.4:+13516777	None:intergenic
TGCTCAGCACCATTATGTGT+TGG	0.508551	5.4:+13516502	None:intergenic
CAACAACTGAACGAAGGAAA+GGG	0.509285	5.4:-13516481	MS.gene59460:CDS
TGCTGGCAGCCACTCTGTTG+AGG	0.514338	5.4:-13516614	MS.gene59460:CDS
CTGTTCATCGCTAGCTCCAA+AGG	0.516724	5.4:+13517189	None:intergenic
GTGGCGAAGCTCTGAGCCAT+GGG	0.518131	5.4:+13516812	None:intergenic
CTCACAAAACCACTCAAAAG+AGG	0.520841	5.4:-13517009	MS.gene59460:CDS
CTTTCAGGGTAAGGATATCT+GGG	0.523976	5.4:-13516974	MS.gene59460:CDS
CCCTTGCTAATACTTTGTGC+AGG	0.524525	5.4:+13516724	None:intergenic
AACAAACCATGATCCAAGCT+TGG	0.527355	5.4:-13517368	MS.gene59460:CDS
AGAAAGTGGGTCAGGCAGAA+GGG	0.531173	5.4:-13516457	MS.gene59460:CDS
GAGGATTGGGCATCAAATGC+GGG	0.535472	5.4:-13516905	MS.gene59460:CDS
AGATTTGGTTCCAATCGTCC+AGG	0.541054	5.4:-13516578	MS.gene59460:CDS
TCCTGCACAAAGTATTAGCA+AGG	0.541782	5.4:-13516725	MS.gene59460:CDS
ATGTTCCTGTCAAAACTCCA+TGG	0.543440	5.4:+13517246	None:intergenic
CACATTTCATCTGCTCCGAT+CGG	0.545180	5.4:+13517468	None:intergenic
CAAGCTACGATCCGAAGCTA+TGG	0.549236	5.4:-13517130	MS.gene59460:CDS
CGTTCCCGAAGATGCGATTG+AGG	0.550162	5.4:+13517217	None:intergenic
TATGGAGAAGGACGAAGAAG+TGG	0.572008	5.4:-13516683	MS.gene59460:CDS
CCATTTCTTCGAAGTCTCCA+AGG	0.574983	5.4:+13517088	None:intergenic
AAGATCAAAATTATGGCCCA+TGG	0.577299	5.4:-13516828	MS.gene59460:CDS
GGGTCGATTACAAGTATGAA+AGG	0.583661	5.4:-13516954	MS.gene59460:CDS
AGTTTCTCCCGTACTCTCGT+CGG	0.587268	5.4:-13517432	MS.gene59460:CDS
TGATCCAAGCTTGGAGAGCG+AGG	0.592427	5.4:-13517359	MS.gene59460:CDS
ATCGTCCAGGAAGATGTAGA+AGG	0.594286	5.4:-13516565	MS.gene59460:CDS
GCTTCGGATCGTAGCTTGAG+TGG	0.594697	5.4:+13517135	None:intergenic
TTCAGGTACAACTATGGAGA+AGG	0.596941	5.4:-13516695	MS.gene59460:CDS
TCTGTTGAGGCAGCGAAAGT+GGG	0.597022	5.4:-13516601	MS.gene59460:CDS
GCAACAACTGAACGAAGGAA+AGG	0.597187	5.4:-13516482	MS.gene59460:CDS
CTTCGGATCGTAGCTTGAGT+GGG	0.604914	5.4:+13517136	None:intergenic
AGTAAAGAGTTAGGTTCCAA+TGG	0.605841	5.4:-13516766	MS.gene59460:CDS
TGTTCCTGTCAAAACTCCAT+GGG	0.606502	5.4:+13517247	None:intergenic
AACAACTGAACGAAGGAAAG+GGG	0.610637	5.4:-13516480	MS.gene59460:CDS
GGAGGATTGGGCATCAAATG+CGG	0.611693	5.4:-13516906	MS.gene59460:CDS
GATGATGAGGCAATACCGAT+CGG	0.612336	5.4:-13517483	MS.gene59460:CDS
AGGCGTGGCTGAATCTTTGG+GGG	0.614059	5.4:-13516545	MS.gene59460:CDS
CGGATCGTAGCTTGAGTGGG+AGG	0.615084	5.4:+13517139	None:intergenic
CCAGGAAGATGTAGAAGGCG+TGG	0.624846	5.4:-13516560	MS.gene59460:CDS
AATAACAAGAGAATCACTGC+TGG	0.626038	5.4:-13516631	MS.gene59460:CDS
TGAAGGAGATAAATAGAATG+GGG	0.630737	5.4:-13517062	MS.gene59460:CDS
ACCAATTGCTGATGATGATG+AGG	0.635568	5.4:-13517496	MS.gene59460:CDS
CCTGCACAAAGTATTAGCAA+GGG	0.637215	5.4:-13516724	MS.gene59460:CDS
CGAAGGAAAGGGGAGAAAGT+GGG	0.639167	5.4:-13516470	MS.gene59460:CDS
TGGTATGCAAGAATGGCCCA+TGG	0.640731	5.4:-13517263	MS.gene59460:CDS
GTCGTAGACTCTGATCCAGA+AGG	0.651411	5.4:+13517161	None:intergenic
AGACTTAGAAGACCATGACC+AGG	0.655835	5.4:-13516875	MS.gene59460:CDS
CTTTCGCTGCCTCAACAGAG+TGG	0.659021	5.4:+13516605	None:intergenic
AAGTGGGTCAGGCAGAAGGG+AGG	0.668050	5.4:-13516454	None:intergenic
GTAGACTCTGATCCAGAAGG+AGG	0.687827	5.4:+13517164	None:intergenic
GCTGAGCAACAACTGAACGA+AGG	0.712386	5.4:-13516487	MS.gene59460:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGAAGGAGATAAATAGAAT+GGG	-	chr5.4:13516911-13516930	MS.gene59460:CDS	25.0%
!	GAAGAGAAAGATCAAAATTA+TGG	-	chr5.4:13517139-13517158	MS.gene59460:CDS	25.0%
!	TATGAAGGAGATAAATAGAA+TGG	-	chr5.4:13516910-13516929	MS.gene59460:CDS	25.0%
!!	AGAGATTTTTGTTCAAATCT+TGG	+	chr5.4:13516655-13516674	None:intergenic	25.0%
	CTGCAGACAAAACAAAAATT+GGG	+	chr5.4:13517049-13517068	None:intergenic	30.0%
	TCTTTGATGTGAACTTGAAA+AGG	+	chr5.4:13516679-13516698	None:intergenic	30.0%
	TGAAGGAGATAAATAGAATG+GGG	-	chr5.4:13516912-13516931	MS.gene59460:CDS	30.0%
	TTCAAATCTTGGATTTCGAT+CGG	+	chr5.4:13516644-13516663	None:intergenic	30.0%
!	TAGATTTTTACTACAGCCAT+TGG	+	chr5.4:13517227-13517246	None:intergenic	30.0%
!	TTGTTATTCTGCTCAACATT+TGG	+	chr5.4:13517331-13517350	None:intergenic	30.0%
!!!	TAGTTTTTTTGCCATAGCTT+CGG	+	chr5.4:13516858-13516877	None:intergenic	30.0%
	AAAAAACTAGGCAACATCCT+TGG	-	chr5.4:13516869-13516888	MS.gene59460:CDS	35.0%
	AAGATCAAAATTATGGCCCA+TGG	-	chr5.4:13517146-13517165	MS.gene59460:CDS	35.0%
	AATAACAAGAGAATCACTGC+TGG	-	chr5.4:13517343-13517362	MS.gene59460:CDS	35.0%
	AGTAAAGAGTTAGGTTCCAA+TGG	-	chr5.4:13517208-13517227	MS.gene59460:CDS	35.0%
	CCTGCAGACAAAACAAAAAT+TGG	+	chr5.4:13517050-13517069	None:intergenic	35.0%
	CTTCGAAGAAATGGATATGA+AGG	-	chr5.4:13516895-13516914	MS.gene59460:CDS	35.0%
	GAAGCTATGGCAAAAAAACT+AGG	-	chr5.4:13516857-13516876	MS.gene59460:CDS	35.0%
	TAACTCTTTACTTGCTTCGA+AGG	+	chr5.4:13517200-13517219	None:intergenic	35.0%
	TCAAATCTTGGATTTCGATC+GGG	+	chr5.4:13516643-13516662	None:intergenic	35.0%
	TTCGAAGCAAGTAAAGAGTT+AGG	-	chr5.4:13517199-13517218	MS.gene59460:CDS	35.0%
!	TTGCTTCGAAGGTGATTTTT+GGG	+	chr5.4:13517189-13517208	None:intergenic	35.0%
!!	TTGGATCATGGTTTGTTTGA+AGG	+	chr5.4:13516603-13516622	None:intergenic	35.0%
!!!	ATTTTTGTTTTGTCTGCAGG+AGG	-	chr5.4:13517050-13517069	MS.gene59460:CDS	35.0%
!!!	CCAATTTTTGTTTTGTCTGC+AGG	-	chr5.4:13517047-13517066	MS.gene59460:CDS	35.0%
!!!	TGCTCAACATTTGGTTCTTT+TGG	+	chr5.4:13517322-13517341	None:intergenic	35.0%
	AACAAACCATGATCCAAGCT+TGG	-	chr5.4:13516606-13516625	MS.gene59460:CDS	40.0%
	AACAACTGAACGAAGGAAAG+GGG	-	chr5.4:13517494-13517513	MS.gene59460:CDS	40.0%
	ACTTTCAGGGTAAGGATATC+TGG	-	chr5.4:13516999-13517018	MS.gene59460:CDS	40.0%
	ATCAAAGAGAGAAGCAGATC+TGG	-	chr5.4:13516691-13516710	MS.gene59460:CDS	40.0%
	ATGTTCCTGTCAAAACTCCA+TGG	+	chr5.4:13516731-13516750	None:intergenic	40.0%
	CAACAACTGAACGAAGGAAA+GGG	-	chr5.4:13517493-13517512	MS.gene59460:CDS	40.0%
	CCAGAATTCAGGTACAACTA+TGG	-	chr5.4:13517273-13517292	MS.gene59460:CDS	40.0%
	CCATAGTTGTACCTGAATTC+TGG	+	chr5.4:13517276-13517295	None:intergenic	40.0%
	CTCACAAAACCACTCAAAAG+AGG	-	chr5.4:13516965-13516984	MS.gene59460:CDS	40.0%
	CTTTCAGGGTAAGGATATCT+GGG	-	chr5.4:13517000-13517019	MS.gene59460:CDS	40.0%
	GGGTCGATTACAAGTATGAA+AGG	-	chr5.4:13517020-13517039	MS.gene59460:CDS	40.0%
	TCTACTCTCCCAACACATAA+TGG	-	chr5.4:13517463-13517482	MS.gene59460:CDS	40.0%
	TGTTCCTGTCAAAACTCCAT+GGG	+	chr5.4:13516730-13516749	None:intergenic	40.0%
	TTCAGGTACAACTATGGAGA+AGG	-	chr5.4:13517279-13517298	MS.gene59460:CDS	40.0%
!	ACCAATTGCTGATGATGATG+AGG	-	chr5.4:13516478-13516497	MS.gene59460:CDS	40.0%
!	ATCATCAGCAATTGGTCTCT+TGG	+	chr5.4:13516474-13516493	None:intergenic	40.0%
!	CCTGCACAAAGTATTAGCAA+GGG	-	chr5.4:13517250-13517269	MS.gene59460:CDS	40.0%
!	CTTGCTTCGAAGGTGATTTT+TGG	+	chr5.4:13517190-13517209	None:intergenic	40.0%
!	TCCTGCACAAAGTATTAGCA+AGG	-	chr5.4:13517249-13517268	MS.gene59460:CDS	40.0%
!	TGAAGGCTCTAGTGTTGTAT+GGG	+	chr5.4:13516586-13516605	None:intergenic	40.0%
!	TTGAAGGCTCTAGTGTTGTA+TGG	+	chr5.4:13516587-13516606	None:intergenic	40.0%
!!!	CTCTTTTGAGTGGTTTTGTG+AGG	+	chr5.4:13516967-13516986	None:intergenic	40.0%
	AGACTTAGAAGACCATGACC+AGG	-	chr5.4:13517099-13517118	MS.gene59460:CDS	45.0%
	ATCGTCCAGGAAGATGTAGA+AGG	-	chr5.4:13517409-13517428	MS.gene59460:CDS	45.0%
	ATTAGCAAGGGCCAGAATTC+AGG	-	chr5.4:13517262-13517281	MS.gene59460:CDS	45.0%
	CAAACTGCACTTTCAGGGTA+AGG	-	chr5.4:13516991-13517010	MS.gene59460:CDS	45.0%
	CACATTTCATCTGCTCCGAT+CGG	+	chr5.4:13516509-13516528	None:intergenic	45.0%
	CACTGAAATCTTCCTGGTCA+TGG	+	chr5.4:13517114-13517133	None:intergenic	45.0%
	CAGCTTCACTGAAATCTTCC+TGG	+	chr5.4:13517120-13517139	None:intergenic	45.0%
	CCATTTCTTCGAAGTCTCCA+AGG	+	chr5.4:13516889-13516908	None:intergenic	45.0%
	CCCTTGCTAATACTTTGTGC+AGG	+	chr5.4:13517253-13517272	None:intergenic	45.0%
	CCTTGGAGACTTCGAAGAAA+TGG	-	chr5.4:13516886-13516905	MS.gene59460:CDS	45.0%
	CTAGTCCTCAATCGCATCTT+CGG	-	chr5.4:13516752-13516771	MS.gene59460:CDS	45.0%
	GATGATGAGGCAATACCGAT+CGG	-	chr5.4:13516491-13516510	MS.gene59460:CDS	45.0%
	GCAACAACTGAACGAAGGAA+AGG	-	chr5.4:13517492-13517511	MS.gene59460:CDS	45.0%
	GCAGATCTGGTATGCAAGAA+TGG	-	chr5.4:13516704-13516723	MS.gene59460:CDS	45.0%
	GCCTCATCATCATCAGCAAT+TGG	+	chr5.4:13516482-13516501	None:intergenic	45.0%
	GCTCAGCACCATTATGTGTT+GGG	+	chr5.4:13517474-13517493	None:intergenic	45.0%
	TAGTCCTCAATCGCATCTTC+GGG	-	chr5.4:13516753-13516772	MS.gene59460:CDS	45.0%
	TATGGAGAAGGACGAAGAAG+TGG	-	chr5.4:13517291-13517310	MS.gene59460:CDS	45.0%
	TCTACATCTTCCTGGACGAT+TGG	+	chr5.4:13517409-13517428	None:intergenic	45.0%
	TGCTCAGCACCATTATGTGT+TGG	+	chr5.4:13517475-13517494	None:intergenic	45.0%
!	AGAGTTTTCCGACGAGAGTA+CGG	+	chr5.4:13516553-13516572	None:intergenic	45.0%
!	AGATTTGGTTCCAATCGTCC+AGG	-	chr5.4:13517396-13517415	MS.gene59460:CDS	45.0%
!	CTTACCCTGAAAGTGCAGTT+TGG	+	chr5.4:13516993-13517012	None:intergenic	45.0%
!	GTTTTGTCTGCAGGAGGATT+GGG	-	chr5.4:13517056-13517075	MS.gene59460:CDS	45.0%
!	TGTTTTGTCTGCAGGAGGAT+TGG	-	chr5.4:13517055-13517074	MS.gene59460:CDS	45.0%
!!	AGTTTGGAGCCTCTTTTGAG+TGG	+	chr5.4:13516977-13516996	None:intergenic	45.0%
!!	TCGAAGGTGATTTTTGGGAG+TGG	+	chr5.4:13517184-13517203	None:intergenic	45.0%
	AAGGCGTGGCTGAATCTTTG+GGG	-	chr5.4:13517428-13517447	MS.gene59460:CDS	50.0%
	ACGAAGGAAAGGGGAGAAAG+TGG	-	chr5.4:13517503-13517522	MS.gene59460:CDS	50.0%
	AGAAAGTGGGTCAGGCAGAA+GGG	-	chr5.4:13517517-13517536	MS.gene59460:CDS	50.0%
	AGGCTCCAAACTGCACTTTC+AGG	-	chr5.4:13516985-13517004	MS.gene59460:CDS	50.0%
	AGTTTCTCCCGTACTCTCGT+CGG	-	chr5.4:13516542-13516561	MS.gene59460:CDS	50.0%
	CAAGCTACGATCCGAAGCTA+TGG	-	chr5.4:13516844-13516863	MS.gene59460:CDS	50.0%
	CGAAGATGCGATTGAGGACT+AGG	+	chr5.4:13516754-13516773	None:intergenic	50.0%
	CGAAGGAAAGGGGAGAAAGT+GGG	-	chr5.4:13517504-13517523	MS.gene59460:CDS	50.0%
	CGATGAACAGATCCTCCTTC+TGG	-	chr5.4:13516798-13516817	MS.gene59460:CDS	50.0%
	CTGTTCATCGCTAGCTCCAA+AGG	+	chr5.4:13516788-13516807	None:intergenic	50.0%
	CTTCGGATCGTAGCTTGAGT+GGG	+	chr5.4:13516841-13516860	None:intergenic	50.0%
	GAGGATTGGGCATCAAATGC+GGG	-	chr5.4:13517069-13517088	MS.gene59460:CDS	50.0%
	GCTGAGCAACAACTGAACGA+AGG	-	chr5.4:13517487-13517506	MS.gene59460:CDS	50.0%
	GGAGGATTGGGCATCAAATG+CGG	-	chr5.4:13517068-13517087	MS.gene59460:CDS	50.0%
	GGCTCCAAACTGCACTTTCA+GGG	-	chr5.4:13516986-13517005	MS.gene59460:CDS	50.0%
	GTACTCTCGTCGGAAAACTC+TGG	-	chr5.4:13516552-13516571	MS.gene59460:CDS	50.0%
	GTAGACTCTGATCCAGAAGG+AGG	+	chr5.4:13516813-13516832	None:intergenic	50.0%
	GTCGTAGACTCTGATCCAGA+AGG	+	chr5.4:13516816-13516835	None:intergenic	50.0%
	TCTCAGAATCAGAGCCTCTC+TGG	-	chr5.4:13516940-13516959	MS.gene59460:CDS	50.0%
	TCTGTTGAGGCAGCGAAAGT+GGG	-	chr5.4:13517373-13517392	MS.gene59460:CDS	50.0%
	TGGTATGCAAGAATGGCCCA+TGG	-	chr5.4:13516711-13516730	MS.gene59460:CDS	50.0%
!	AGAAGGCGTGGCTGAATCTT+TGG	-	chr5.4:13517426-13517445	MS.gene59460:CDS	50.0%
!	GAAGGCGTGGCTGAATCTTT+GGG	-	chr5.4:13517427-13517446	MS.gene59460:CDS	50.0%
!!	ATGGCCCATGGAGTTTTGAC+AGG	-	chr5.4:13516723-13516742	MS.gene59460:CDS	50.0%
!!	GAGTTTTCCGACGAGAGTAC+GGG	+	chr5.4:13516552-13516571	None:intergenic	50.0%
!!!	TGGTTTTGTGAGGTCCAGAG+AGG	+	chr5.4:13516957-13516976	None:intergenic	50.0%
	AGGCGTGGCTGAATCTTTGG+GGG	-	chr5.4:13517429-13517448	MS.gene59460:CDS	55.0%
	CCACGCCTTCTACATCTTCC+TGG	+	chr5.4:13517417-13517436	None:intergenic	55.0%
	CCAGGAAGATGTAGAAGGCG+TGG	-	chr5.4:13517414-13517433	MS.gene59460:CDS	55.0%
	CGCTCTCCAAGCTTGGATCA+TGG	+	chr5.4:13516615-13516634	None:intergenic	55.0%
	CGTTCCCGAAGATGCGATTG+AGG	+	chr5.4:13516760-13516779	None:intergenic	55.0%
	CTCTGTTGAGGCAGCGAAAG+TGG	-	chr5.4:13517372-13517391	MS.gene59460:CDS	55.0%
	CTTCGGGAACGAGCAACCTT+TGG	-	chr5.4:13516769-13516788	MS.gene59460:CDS	55.0%
	CTTTCGCTGCCTCAACAGAG+TGG	+	chr5.4:13517372-13517391	None:intergenic	55.0%
	GAAAGGGGAGAAAGTGGGTC+AGG	-	chr5.4:13517509-13517528	MS.gene59460:CDS	55.0%
	GAGAAAGTGGGTCAGGCAGA+AGG	-	chr5.4:13517516-13517535	MS.gene59460:CDS	55.0%
	GCTTCGGATCGTAGCTTGAG+TGG	+	chr5.4:13516842-13516861	None:intergenic	55.0%
	GGCAGCGAAAGTGGGAGATT+TGG	-	chr5.4:13517381-13517400	MS.gene59460:CDS	55.0%
	TGATCCAAGCTTGGAGAGCG+AGG	-	chr5.4:13516615-13516634	MS.gene59460:CDS	55.0%
	CGGATCGTAGCTTGAGTGGG+AGG	+	chr5.4:13516838-13516857	None:intergenic	60.0%
	GGTTCCTCGCTCTCCAAGCT+TGG	+	chr5.4:13516622-13516641	None:intergenic	60.0%
	TGCTGGCAGCCACTCTGTTG+AGG	-	chr5.4:13517360-13517379	MS.gene59460:CDS	60.0%
!	CGTGGCTGAATCTTTGGGGG+CGG	-	chr5.4:13517432-13517451	MS.gene59460:CDS	60.0%
!!	AGTGGCGAAGCTCTGAGCCA+TGG	+	chr5.4:13517166-13517185	None:intergenic	60.0%
!!	GTGGCGAAGCTCTGAGCCAT+GGG	+	chr5.4:13517165-13517184	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.4	gene	13516455	13517541	13516455	ID=MS.gene59460
chr5.4	mRNA	13516455	13517541	13516455	ID=MS.gene59460.t1;Parent=MS.gene59460
chr5.4	exon	13516455	13517541	13516455	ID=MS.gene59460.t1.exon1;Parent=MS.gene59460.t1
chr5.4	CDS	13516455	13517541	13516455	ID=cds.MS.gene59460.t1;Parent=MS.gene59460.t1

Gene Sequence

>MS.gene59460

ATGGAGGGAAACAAAACCAAGAGACCAATTGCTGATGATGATGAGGCAATACCGATCGGAGCAGATGAAATGTGTAACGACGATGAGAGTTTCTCCCGTACTCTCGTCGGAAAACTCTGGACAGAAATCCCATACAACACTAGAGCCTTCAAACAAACCATGATCCAAGCTTGGAGAGCGAGGAACCCGATCGAAATCCAAGATTTGAACAAAAATCTCTTCCTTTTCAAGTTCACATCAAAGAGAGAAGCAGATCTGGTATGCAAGAATGGCCCATGGAGTTTTGACAGGAACATCCTAGTCCTCAATCGCATCTTCGGGAACGAGCAACCTTTGGAGCTAGCGATGAACAGATCCTCCTTCTGGATCAGAGTCTACGACCTCCCACTCAAGCTACGATCCGAAGCTATGGCAAAAAAACTAGGCAACATCCTTGGAGACTTCGAAGAAATGGATATGAAGGAGATAAATAGAATGGGGAAGTTTCTCAGAATCAGAGCCTCTCTGGACCTCACAAAACCACTCAAAAGAGGCTCCAAACTGCACTTTCAGGGTAAGGATATCTGGGTCGATTACAAGTATGAAAGGCTACCCAATTTTTGTTTTGTCTGCAGGAGGATTGGGCATCAAATGCGGGACTGTGAAGACTTAGAAGACCATGACCAGGAAGATTTCAGTGAAGCTGAAGAGAAAGATCAAAATTATGGCCCATGGCTCAGAGCTTCGCCACTCCCAAAAATCACCTTCGAAGCAAGTAAAGAGTTAGGTTCCAATGGCTGTAGTAAAAATCTATTTCCTGCACAAAGTATTAGCAAGGGCCAGAATTCAGGTACAACTATGGAGAAGGACGAAGAAGTGGAGCAACCAAAAGAACCAAATGTTGAGCAGAATAACAAGAGAATCACTGCTGGCAGCCACTCTGTTGAGGCAGCGAAAGTGGGAGATTTGGTTCCAATCGTCCAGGAAGATGTAGAAGGCGTGGCTGAATCTTTGGGGGCGGTCTCAATCTCTACTCTCCCAACACATAATGGTGCTGAGCAACAACTGAACGAAGGAAAGGGGAGAAAGTGGGTCAGGCAGAAGGGAG

Protein sequence

>MS.gene59460.t1

MEGNKTKRPIADDDEAIPIGADEMCNDDESFSRTLVGKLWTEIPYNTRAFKQTMIQAWRARNPIEIQDLNKNLFLFKFTSKREADLVCKNGPWSFDRNILVLNRIFGNEQPLELAMNRSSFWIRVYDLPLKLRSEAMAKKLGNILGDFEEMDMKEINRMGKFLRIRASLDLTKPLKRGSKLHFQGKDIWVDYKYERLPNFCFVCRRIGHQMRDCEDLEDHDQEDFSEAEEKDQNYGPWLRASPLPKITFEASKELGSNGCSKNLFPAQSISKGQNSGTTMEKDEEVEQPKEPNVEQNNKRITAGSHSVEAAKVGDLVPIVQEDVEGVAESLGAVSISTLPTHNGAEQQLNEGKGRKWVRQKG