AlfalfaGEDB | MS.gene017586

M. sativa cultivar XinJiangDaYe / MS.gene017586

KEGG: -
GO: GO:0003674 GO:0003676 GO:0005488 GO:0008270 GO:0043167 GO:0043169 GO:0046872 GO:0046914 GO:0097159 GO:1901363
PFAM: PF14111.9 PF14392.9

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017586.t1	AES70414.1	53.8	346	91	4	1	343	1	280	2.70E-91	345.9

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017586.t1	O04244	25.7	136	97	2	83	216	6	139	2.9e-09	64.7

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017586.t1	G7J017	53.8	346	91	4	1	343	1	280	2.0e-91	345.9

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene042559	MS.gene017586	PPI
MS.gene017586	MS.gene062511	PPI
MS.gene22200	MS.gene017586	PPI
MS.gene017586	MS.gene059269	PPI
MS.gene26038	MS.gene017586	PPI
MS.gene017586	MS.gene024841	PPI
MS.gene85308	MS.gene017586	PPI
MS.gene031967	MS.gene017586	PPI
MS.gene017586	MS.gene46353	PPI
MS.gene017586	MS.gene40828	PPI
MS.gene34812	MS.gene017586	PPI
MS.gene02896	MS.gene017586	PPI
MS.gene017586	MS.gene51799	PPI
MS.gene063214	MS.gene017586	PPI
MS.gene55682	MS.gene017586	PPI
MS.gene97410	MS.gene017586	PPI
MS.gene32415	MS.gene017586	PPI
MS.gene017586	MS.gene61651	PPI
MS.gene035626	MS.gene017586	PPI
MS.gene067340	MS.gene017586	PPI
MS.gene65623	MS.gene017586	PPI
MS.gene017586	MS.gene09023	PPI
MS.gene017586	MS.gene054508	PPI
MS.gene71675	MS.gene017586	PPI
MS.gene017586	MS.gene025987	PPI
MS.gene69495	MS.gene017586	PPI
MS.gene017586	MS.gene95960	PPI
MS.gene049512	MS.gene017586	PPI
MS.gene017586	MS.gene043140	PPI
MS.gene038623	MS.gene017586	PPI
MS.gene017586	MS.gene03149	PPI
MS.gene05357	MS.gene017586	PPI
MS.gene032311	MS.gene017586	PPI
MS.gene96174	MS.gene017586	PPI
MS.gene017586	MS.gene29321	PPI
MS.gene86849	MS.gene017586	PPI
MS.gene017586	MS.gene025401	PPI
MS.gene072852	MS.gene017586	PPI
MS.gene017586	MS.gene015093	PPI
MS.gene017586	MS.gene24278	PPI
MS.gene017586	MS.gene000379	PPI
MS.gene39956	MS.gene017586	PPI
MS.gene017586	MS.gene34618	PPI
MS.gene017586	MS.gene59460	PPI
MS.gene021968	MS.gene017586	PPI
MS.gene017586	MS.gene66650	PPI
MS.gene09589	MS.gene017586	PPI
MS.gene017586	MS.gene08713	PPI
MS.gene43393	MS.gene017586	PPI
MS.gene017586	MS.gene09517	PPI
MS.gene017586	MS.gene87845	PPI
MS.gene057828	MS.gene017586	PPI
MS.gene34197	MS.gene017586	PPI
MS.gene66650	MS.gene017586	PPI
MS.gene005120	MS.gene017586	PPI
MS.gene017586	MS.gene64178	PPI
MS.gene048072	MS.gene017586	PPI
MS.gene060386	MS.gene017586	PPI
MS.gene71588	MS.gene017586	PPI
MS.gene04749	MS.gene017586	PPI
MS.gene017586	MS.gene44144	PPI
MS.gene05436	MS.gene017586	PPI
MS.gene017586	MS.gene060718	PPI
MS.gene059736	MS.gene017586	PPI
MS.gene70932	MS.gene017586	PPI
MS.gene35175	MS.gene017586	PPI
MS.gene017586	MS.gene027169	PPI
MS.gene017586	MS.gene004417	PPI
MS.gene017586	MS.gene019177	PPI
MS.gene021965	MS.gene017586	PPI
MS.gene055683	MS.gene017586	PPI
MS.gene042614	MS.gene017586	PPI
MS.gene41643	MS.gene017586	PPI
MS.gene26844	MS.gene017586	PPI
MS.gene021555	MS.gene017586	PPI
MS.gene021647	MS.gene017586	PPI
MS.gene96109	MS.gene017586	PPI
MS.gene017586	MS.gene055560	PPI
MS.gene017586	MS.gene024297	PPI
MS.gene017586	MS.gene057934	PPI
MS.gene017586	MS.gene37537	PPI
MS.gene017586	MS.gene069786	PPI
MS.gene009121	MS.gene017586	PPI
MS.gene94654	MS.gene017586	PPI
MS.gene017586	MS.gene42010	PPI
MS.gene017586	MS.gene025158	PPI
MS.gene51804	MS.gene017586	PPI
MS.gene36069	MS.gene017586	PPI
MS.gene017586	MS.gene27329	PPI
MS.gene017586	MS.gene41393	PPI
MS.gene83772	MS.gene017586	PPI
MS.gene017586	MS.gene016632	PPI
MS.gene017586	MS.gene018474	PPI
MS.gene008927	MS.gene017586	PPI
MS.gene016102	MS.gene017586	PPI
MS.gene045614	MS.gene017586	PPI
MS.gene96541	MS.gene017586	PPI
MS.gene000634	MS.gene017586	PPI
MS.gene63762	MS.gene017586	PPI
MS.gene017586	MS.gene40469	PPI
MS.gene017586	MS.gene96163	PPI
MS.gene30208	MS.gene017586	PPI
MS.gene025873	MS.gene017586	PPI
MS.gene013676	MS.gene017586	PPI
MS.gene05358	MS.gene017586	PPI
MS.gene030090	MS.gene017586	PPI
MS.gene95561	MS.gene017586	PPI
MS.gene074257	MS.gene017586	PPI
MS.gene018196	MS.gene017586	PPI
MS.gene065627	MS.gene017586	PPI
MS.gene017586	MS.gene037049	PPI
MS.gene57019	MS.gene017586	PPI
MS.gene018745	MS.gene017586	PPI
MS.gene65546	MS.gene017586	PPI
MS.gene017586	MS.gene61499	PPI
MS.gene03936	MS.gene017586	PPI
MS.gene017586	MS.gene019165	PPI
MS.gene017586	MS.gene81194	PPI
MS.gene038098	MS.gene017586	PPI
MS.gene017586	MS.gene060773	PPI
MS.gene60059	MS.gene017586	PPI
MS.gene017586	MS.gene038394	PPI
MS.gene28545	MS.gene017586	PPI
MS.gene017586	MS.gene019001	PPI
MS.gene28718	MS.gene017586	PPI
MS.gene055560	MS.gene017586	PPI
MS.gene017586	MS.gene53904	PPI
MS.gene008275	MS.gene017586	PPI
MS.gene017586	MS.gene24070	PPI
MS.gene017586	MS.gene73261	PPI
MS.gene017586	MS.gene85538	PPI
MS.gene038757	MS.gene017586	PPI
MS.gene017586	MS.gene025675	PPI
MS.gene064966	MS.gene017586	PPI
MS.gene017586	MS.gene26844	PPI
MS.gene017586	MS.gene055546	PPI
MS.gene017586	MS.gene55209	PPI
MS.gene017586	MS.gene074257	PPI
MS.gene017586	MS.gene073573	PPI
MS.gene40828	MS.gene017586	PPI
MS.gene43924	MS.gene017586	PPI
MS.gene046089	MS.gene017586	PPI
MS.gene047954	MS.gene017586	PPI
MS.gene036423	MS.gene017586	PPI
MS.gene017586	MS.gene068629	PPI
MS.gene017586	MS.gene009781	PPI
MS.gene013644	MS.gene017586	PPI
MS.gene72520	MS.gene017586	PPI
MS.gene66651	MS.gene017586	PPI
MS.gene017586	MS.gene21066	PPI
MS.gene017586	MS.gene009213	PPI
MS.gene35202	MS.gene017586	PPI
MS.gene017586	MS.gene013890	PPI
MS.gene051449	MS.gene017586	PPI
MS.gene017586	MS.gene98127	PPI
MS.gene017586	MS.gene003264	PPI
MS.gene017586	MS.gene054652	PPI
MS.gene63743	MS.gene017586	PPI
MS.gene48477	MS.gene017586	PPI
MS.gene017586	MS.gene45083	PPI
MS.gene045875	MS.gene017586	PPI
MS.gene017586	MS.gene049561	PPI
MS.gene017586	MS.gene008658	PPI
MS.gene30136	MS.gene017586	PPI
MS.gene017586	MS.gene49057	PPI
MS.gene008861	MS.gene017586	PPI
MS.gene61452	MS.gene017586	PPI
MS.gene017586	MS.gene89815	PPI
MS.gene017586	MS.gene048088	PPI
MS.gene017586	MS.gene86868	PPI
MS.gene83979	MS.gene017586	PPI
MS.gene46941	MS.gene017586	PPI
MS.gene85295	MS.gene017586	PPI
MS.gene017586	MS.gene058889	PPI
MS.gene47792	MS.gene017586	PPI
MS.gene46948	MS.gene017586	PPI
MS.gene98471	MS.gene017586	PPI
MS.gene012019	MS.gene017586	PPI
MS.gene93396	MS.gene017586	PPI
MS.gene017586	MS.gene012889	PPI
MS.gene017586	MS.gene34463	PPI
MS.gene017586	MS.gene000506	PPI
MS.gene05231	MS.gene017586	PPI
MS.gene010656	MS.gene017586	PPI
MS.gene000629	MS.gene017586	PPI
MS.gene041264	MS.gene017586	PPI
MS.gene41979	MS.gene017586	PPI
MS.gene017586	MS.gene51804	PPI
MS.gene017586	MS.gene013644	PPI
MS.gene64130	MS.gene017586	PPI
MS.gene05319	MS.gene017586	PPI
MS.gene56991	MS.gene017586	PPI
MS.gene050679	MS.gene017586	PPI
MS.gene20246	MS.gene017586	PPI
MS.gene055223	MS.gene017586	PPI
MS.gene053747	MS.gene017586	PPI
MS.gene65471	MS.gene017586	PPI
MS.gene061850	MS.gene017586	PPI
MS.gene26433	MS.gene017586	PPI
MS.gene017586	MS.gene060788	PPI
MS.gene017586	MS.gene41643	PPI
MS.gene073582	MS.gene017586	PPI
MS.gene058889	MS.gene017586	PPI
MS.gene017586	MS.gene027994	PPI
MS.gene98616	MS.gene017586	PPI
MS.gene017586	MS.gene022929	PPI
MS.gene017586	MS.gene007022	PPI
MS.gene042027	MS.gene017586	PPI
MS.gene017586	MS.gene69584	PPI
MS.gene017586	MS.gene52349	PPI
MS.gene69490	MS.gene017586	PPI
MS.gene015248	MS.gene017586	PPI
MS.gene017586	MS.gene021647	PPI
MS.gene80362	MS.gene017586	PPI
MS.gene017586	MS.gene048342	PPI
MS.gene44083	MS.gene017586	PPI
MS.gene017586	MS.gene069624	PPI
MS.gene013193	MS.gene017586	PPI
MS.gene04970	MS.gene017586	PPI
MS.gene08106	MS.gene017586	PPI
MS.gene58419	MS.gene017586	PPI
MS.gene017586	MS.gene073582	PPI
MS.gene017586	MS.gene016719	PPI
MS.gene017586	MS.gene028714	PPI
MS.gene017586	MS.gene88393	PPI
MS.gene017586	MS.gene048706	PPI
MS.gene23521	MS.gene017586	PPI
MS.gene017586	MS.gene69586	PPI
MS.gene017586	MS.gene053373	PPI
MS.gene29406	MS.gene017586	PPI
MS.gene86275	MS.gene017586	PPI
MS.gene87529	MS.gene017586	PPI
MS.gene024405	MS.gene017586	PPI
MS.gene017586	MS.gene63150	PPI
MS.gene012748	MS.gene017586	PPI
MS.gene04134	MS.gene017586	PPI
MS.gene062067	MS.gene017586	PPI
MS.gene017586	MS.gene072852	PPI
MS.gene017586	MS.gene65474	PPI
MS.gene017586	MS.gene08708	PPI
MS.gene57044	MS.gene017586	PPI
MS.gene017586	MS.gene06164	PPI
MS.gene017586	MS.gene50392	PPI
MS.gene017586	MS.gene86275	PPI
MS.gene017586	MS.gene22730	PPI
MS.gene017586	MS.gene032299	PPI
MS.gene049610	MS.gene017586	PPI
MS.gene038676	MS.gene017586	PPI
MS.gene017586	MS.gene037634	PPI
MS.gene71637	MS.gene017586	PPI
MS.gene017586	MS.gene057828	PPI
MS.gene017586	MS.gene08606	PPI
MS.gene042651	MS.gene017586	PPI
MS.gene017586	MS.gene000640	PPI
MS.gene05576	MS.gene017586	PPI
MS.gene017586	MS.gene98756	PPI
MS.gene98467	MS.gene017586	PPI
MS.gene037049	MS.gene017586	PPI
MS.gene017586	MS.gene028716	PPI
MS.gene017586	MS.gene74639	PPI
MS.gene017586	MS.gene071133	PPI
MS.gene017586	MS.gene60266	PPI
MS.gene034643	MS.gene017586	PPI
MS.gene017586	MS.gene98467	PPI
MS.gene017586	MS.gene62249	PPI
MS.gene017586	MS.gene024522	PPI
MS.gene017586	MS.gene54051	PPI
MS.gene017586	MS.gene22111	PPI
MS.gene017586	MS.gene049610	PPI
MS.gene017586	MS.gene010290	PPI
MS.gene017586	MS.gene37955	PPI
MS.gene017586	MS.gene92517	PPI
MS.gene017586	MS.gene045875	PPI
MS.gene017586	MS.gene010890	PPI
MS.gene017586	MS.gene84790	PPI
MS.gene017586	MS.gene044729	PPI
MS.gene071784	MS.gene017586	PPI
MS.gene061579	MS.gene017586	PPI
MS.gene017586	MS.gene60390	PPI
MS.gene017586	MS.gene039692	PPI
MS.gene76420	MS.gene017586	PPI
MS.gene071320	MS.gene017586	PPI
MS.gene42903	MS.gene017586	PPI
MS.gene81712	MS.gene017586	PPI
MS.gene017586	MS.gene55682	PPI
MS.gene017586	MS.gene002590	PPI
MS.gene026680	MS.gene017586	PPI
MS.gene048747	MS.gene017586	PPI
MS.gene017586	MS.gene77990	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017586.t1	MTR_3g053920	53.757	346	91	4	1	343	1	280	1.15e-115	340
MS.gene017586.t1	MTR_2g061500	77.519	129	29	0	1	129	1	129	1.52e-70	219
MS.gene017586.t1	MTR_7g011710	88.235	85	10	0	55	139	1	85	7.00e-49	164
MS.gene017586.t1	MTR_4g103530	71.287	101	28	1	55	155	1	100	9.47e-46	157
MS.gene017586.t1	MTR_6g033215	35.260	173	110	1	29	199	122	294	1.16e-35	133
MS.gene017586.t1	MTR_0010s0370	34.302	172	107	3	11	177	7	177	1.48e-28	111
MS.gene017586.t1	MTR_0010s0350	34.302	172	107	3	11	177	7	177	1.48e-28	111
MS.gene017586.t1	MTR_8g463110	66.990	103	31	1	289	388	1	103	1.84e-27	115
MS.gene017586.t1	MTR_1g036720	31.731	104	69	1	11	112	7	110	2.51e-13	66.6
MS.gene017586.t1	MTR_7g106180	25.333	150	104	3	32	175	19	166	6.93e-13	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene017586.t1	AT2G17920	26.761	213	150	3	8	216	9	219	9.13e-19	87.0
MS.gene017586.t1	AT5G36228	27.461	193	136	2	36	226	39	229	5.07e-18	85.5
MS.gene017586.t1	AT2G41590	26.829	164	116	2	55	216	58	219	1.46e-16	81.3
MS.gene017586.t1	AT3G57587	28.188	149	101	3	71	216	74	219	7.00e-16	79.3
MS.gene017586.t1	AT5G25200	26.512	215	148	4	8	216	9	219	1.19e-15	78.6
MS.gene017586.t1	AT5G18636	26.512	215	148	4	8	216	9	219	1.36e-15	78.2
MS.gene017586.t1	AT2G02103	26.829	164	116	2	55	216	58	219	1.70e-15	78.2
MS.gene017586.t1	AT2G13450	25.824	182	131	2	36	215	39	218	1.16e-14	75.9
MS.gene017586.t1	AT3G31430	25.824	182	129	3	36	215	134	311	1.27e-14	75.1
MS.gene017586.t1	AT3G47920	25.676	148	106	2	71	216	3	148	5.16e-13	69.7

Find 134 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GACTCCTCAGAGTTGGTAAT+TGG	0.242886	8.3:+28536505	None:intergenic
GTTCCGCTGCTAATAATAAA+TGG	0.253881	8.3:-28536834	MS.gene017586:CDS
GTTTCAATTTCACCACAAAA+TGG	0.274429	8.3:-28537331	MS.gene017586:CDS
CAGACTCGGTGGCTAGGTTA+AGG	0.302707	8.3:+28537502	None:intergenic
GTGTATCTAAACATCGCTTT+AGG	0.328089	8.3:-28535551	MS.gene017586:intron
CTTAGCTGACCGTGAGATTC+AGG	0.332229	8.3:-28537439	MS.gene017586:CDS
CAAGGCATAGGCAGCTTTCT+AGG	0.333453	8.3:-28537141	MS.gene017586:CDS
CTGATGATGGTGTCCGCAAT+TGG	0.338507	8.3:-28536891	MS.gene017586:CDS
GGAGCAACATTAACATAAAT+AGG	0.340295	8.3:+28535588	None:intergenic
GCTGGCCGCCCTTTGGTGCT+TGG	0.347355	8.3:-28536547	MS.gene017586:CDS
TACGGGCAGCATTAGGCTTC+AGG	0.352023	8.3:+28536860	None:intergenic
CTCGGCACAGCAGTAGGAAT+TGG	0.352359	8.3:+28536802	None:intergenic
TTCTGTCGACTTGAACCATT+TGG	0.355812	8.3:-28537220	MS.gene017586:CDS
AGCCATACGCTCACTGAAAT+AGG	0.356045	8.3:+28537413	None:intergenic
GCAGCATTAGGCTTCAGGTC+AGG	0.367623	8.3:+28536865	None:intergenic
CCTATGCCTTGACCAACATT+TGG	0.367628	8.3:+28537153	None:intergenic
CTTACTTGCTGGCCGCCCTT+TGG	0.370582	8.3:-28536554	MS.gene017586:CDS
ACTTGAACCATTTGGACATA+TGG	0.371083	8.3:-28537212	MS.gene017586:CDS
ATCCTTGATCACTGTTTGAT+TGG	0.378099	8.3:-28537468	MS.gene017586:CDS
ACGATCGAAACACGCTTGCC+TGG	0.389839	8.3:+28537378	None:intergenic
CAGGTTCACGGTCTTCCCTT+TGG	0.395747	8.3:-28537186	MS.gene017586:CDS
TCAGCTTCAAACTTACTTGC+TGG	0.403882	8.3:-28536565	MS.gene017586:CDS
AGACTCGGTGGCTAGGTTAA+GGG	0.413831	8.3:+28537503	None:intergenic
CAAAGGGAAGACCGTGAACC+TGG	0.415175	8.3:+28537187	None:intergenic
ATGGCTCGTGCTTGGAAGCC+AGG	0.426942	8.3:-28537396	MS.gene017586:CDS
GAACCAATACGGGCAGCATT+AGG	0.429705	8.3:+28536853	None:intergenic
TGATGATGGTGTCCGCAATT+GGG	0.436404	8.3:-28536890	MS.gene017586:CDS
GGATACGGTCCATTACGATA+TGG	0.437208	8.3:+28537262	None:intergenic
GACATATGGGTCCAGGTTCA+CGG	0.437811	8.3:-28537198	MS.gene017586:CDS
TGTTTCAGGTGTGGTTTGCT+TGG	0.440387	8.3:-28536955	MS.gene017586:CDS
AAGCCTAATGCTGCCCGTAT+TGG	0.443720	8.3:-28536856	MS.gene017586:CDS
CACCACTAGGAGGAGAGTTT+CGG	0.446642	8.3:+28536746	None:intergenic
TTGTTACCTTCTTCTTCTAC+AGG	0.449515	8.3:-28536592	MS.gene017586:CDS
AATAGTGTTACGGACATCAT+AGG	0.456763	8.3:+28537107	None:intergenic
ATGAAATTTCACCATTCTGA+AGG	0.462585	8.3:+28536434	None:intergenic
ATTTCATCATTTGCTGCTGA+TGG	0.466311	8.3:-28536418	MS.gene017586:CDS
TATTAGCAGCGGAACCAATA+CGG	0.472774	8.3:+28536842	None:intergenic
TCGGCACAGCAGTAGGAATT+GGG	0.473536	8.3:+28536803	None:intergenic
AACAAGAAGGTCATGTTTGA+GGG	0.473613	8.3:+28536666	None:intergenic
TTGTCCGGAGTTGTTCGAGC+TGG	0.474275	8.3:-28536917	MS.gene017586:CDS
ACGGACTTTCAGACGCATAT+AGG	0.475542	8.3:+28537077	None:intergenic
TGGCCTCACCCTTGCTGAAG+AGG	0.475784	8.3:-28537532	MS.gene017586:CDS
TTTCATCATTTGCTGCTGAT+GGG	0.478737	8.3:-28536417	MS.gene017586:CDS
GGCCTCACCCTTGCTGAAGA+GGG	0.479040	8.3:-28537531	MS.gene017586:CDS
GCGCAGACGACATGTCAGTT+TGG	0.481046	8.3:-28535428	MS.gene017586:intron
CAACAAGAAGGTCATGTTTG+AGG	0.483349	8.3:+28536665	None:intergenic
CAAACAGTGATCAAGGATAG+TGG	0.483405	8.3:+28537473	None:intergenic
CAGCATTAGGCTTCAGGTCA+GGG	0.485216	8.3:+28536866	None:intergenic
CCTGCTGGTGTCTTTCATGC+TGG	0.485754	8.3:-28536769	MS.gene017586:CDS
GAGGAGTCTCAAGATGCTAC+TGG	0.487924	8.3:-28536490	MS.gene017586:CDS
GTAATTGGAAACGGTTCAGC+AGG	0.488936	8.3:+28536520	None:intergenic
CCTGGACCCATATGTCCAAA+TGG	0.490389	8.3:+28537205	None:intergenic
ACACACTGACAAAGTTTGTC+CGG	0.500003	8.3:-28536932	MS.gene017586:CDS
CCATTTGGACATATGGGTCC+AGG	0.504581	8.3:-28537205	MS.gene017586:CDS
CTTGAACCATTTGGACATAT+GGG	0.505866	8.3:-28537211	MS.gene017586:CDS
CCGAGCCACTATGTTCCTGC+TGG	0.508741	8.3:-28536784	MS.gene017586:CDS
CATAAGCACCATTACTAACT+CGG	0.510210	8.3:+28537016	None:intergenic
CGATAATTTCCATATCGTAA+TGG	0.512245	8.3:-28537271	MS.gene017586:CDS
GTGAGCGTATGGCTCGTGCT+TGG	0.515599	8.3:-28537404	MS.gene017586:CDS
ATTAGCAGCGGAACCAATAC+GGG	0.517620	8.3:+28536843	None:intergenic
GGGTGAGGCCAGCGATATTG+AGG	0.519134	8.3:+28537544	None:intergenic
TCAGAGTTGGTAATTGGAAA+CGG	0.522460	8.3:+28536511	None:intergenic
TGCCTATTTCAGTGAGCGTA+TGG	0.524721	8.3:-28537415	MS.gene017586:CDS
ATTGAGGTGGGAAGGGTCCA+TGG	0.525018	8.3:+28537560	None:intergenic
TTCATCATTTGCTGCTGATG+GGG	0.534420	8.3:-28536416	MS.gene017586:CDS
CTGAACTGAAGAAGAGAAAG+AGG	0.535515	8.3:-28536465	MS.gene017586:CDS
GGTGAAATTGAAACAGAAAA+CGG	0.538716	8.3:+28537340	None:intergenic
AACCCTCTTCAGCAAGGGTG+AGG	0.538870	8.3:+28537529	None:intergenic
AGGTACTATGAATAGTGTTA+CGG	0.539531	8.3:+28537097	None:intergenic
ACAGAAGTGGGGACAACACC+AGG	0.541151	8.3:+28537237	None:intergenic
TTCGAGCTGGAGTCTGATGA+TGG	0.541255	8.3:-28536904	MS.gene017586:CDS
TAGTGGCTCGGCACAGCAGT+AGG	0.541285	8.3:+28536796	None:intergenic
GGCCAATCAAACAGTGATCA+AGG	0.541425	8.3:+28537466	None:intergenic
TGGTTCAAGTCGACAGAAGT+GGG	0.543153	8.3:+28537225	None:intergenic
GGACAACACCAGGCTGGATA+CGG	0.543289	8.3:+28537247	None:intergenic
CCAAATGTTGGTCAAGGCAT+AGG	0.543940	8.3:-28537153	MS.gene017586:CDS
GGTTGCATTCAACCAAATGT+TGG	0.545080	8.3:-28537165	MS.gene017586:CDS
GTCAAAGCGAAACATGGCTC+CGG	0.549165	8.3:-28536631	MS.gene017586:CDS
GACTCCAGCTCGAACAACTC+CGG	0.551808	8.3:+28536913	None:intergenic
TTTGGTTGAATGCAACCAAA+GGG	0.554402	8.3:+28537171	None:intergenic
TCAGGTCAGGGCCCCAATTG+CGG	0.557468	8.3:+28536878	None:intergenic
ATGTTTCAGACTCGGTGGCT+AGG	0.557537	8.3:+28537496	None:intergenic
AAGTGGGGACAACACCAGGC+TGG	0.558202	8.3:+28537241	None:intergenic
GATGATGGTGTCCGCAATTG+GGG	0.560110	8.3:-28536889	MS.gene017586:CDS
GAGGTACCTGTAGAAGAAGA+AGG	0.560396	8.3:+28536586	None:intergenic
CCAGCATGAAAGACACCAGC+AGG	0.562119	8.3:+28536769	None:intergenic
GGGTTAAACCCTCTTCAGCA+AGG	0.563127	8.3:+28537523	None:intergenic
ATCTTGAGACTCCTCAGAGT+TGG	0.563156	8.3:+28536498	None:intergenic
CAGGTAGTCCAAGCACCAAA+GGG	0.564684	8.3:+28536539	None:intergenic
GATACACAAGGCCGTGGTGT+GGG	0.566825	8.3:+28535567	None:intergenic
AGCAAGTAAGTTTGAAGCTG+AGG	0.567236	8.3:+28536567	None:intergenic
GAAGAAGAGAAAGAGGATGC+TGG	0.568456	8.3:-28536458	MS.gene017586:CDS
AAGGAGCGGTGACATTAATA+CGG	0.569148	8.3:+28537058	None:intergenic
CCTTCCCACCTCAATATCGC+TGG	0.569995	8.3:-28537552	MS.gene017586:CDS
ATAGGATAGTGATCAGAAGC+AGG	0.576456	8.3:+28535606	None:intergenic
AACAGTGTCAAAGCGAAACA+TGG	0.576976	8.3:-28536637	MS.gene017586:CDS
GATTGTCAACAGCAAGCATA+CGG	0.578956	8.3:+28536698	None:intergenic
CCAGCGATATTGAGGTGGGA+AGG	0.579726	8.3:+28537552	None:intergenic
CAGCGATATTGAGGTGGGAA+GGG	0.581963	8.3:+28537553	None:intergenic
CGGACCGTCATCAAGAATGC+GGG	0.583338	8.3:+28537302	None:intergenic
CAAGTGTATCTGACAAGCAC+CGG	0.585274	8.3:+28536612	None:intergenic
AGATACACAAGGCCGTGGTG+TGG	0.585352	8.3:+28535566	None:intergenic
ATGGTTCAAGTCGACAGAAG+TGG	0.586579	8.3:+28537224	None:intergenic
AGGATGCTGGCCCTTCAGAA+TGG	0.591615	8.3:-28536445	MS.gene017586:CDS
ATTCAACCAAATGTTGGTCA+AGG	0.595811	8.3:-28537159	MS.gene017586:CDS
TGAAATTTCACCATTCTGAA+GGG	0.596074	8.3:+28536435	None:intergenic
CAACCATTTATTATTAGCAG+CGG	0.602480	8.3:+28536831	None:intergenic
GCTGCCCGCATTCTTGATGA+CGG	0.605798	8.3:-28537306	MS.gene017586:CDS
TGTTTAGATACACAAGGCCG+TGG	0.609248	8.3:+28535561	None:intergenic
ATTTGGTTGAATGCAACCAA+AGG	0.609384	8.3:+28537170	None:intergenic
TGGAAAGTCCGAGTTAGTAA+TGG	0.609448	8.3:-28537024	MS.gene017586:CDS
TGAGGCCAGCGATATTGAGG+TGG	0.610022	8.3:+28537547	None:intergenic
GATAGTGGATGTTTCAGACT+CGG	0.611459	8.3:+28537488	None:intergenic
GCATTCTTGATGACGGTCCG+TGG	0.613963	8.3:-28537299	MS.gene017586:CDS
GGTTAAACCCTCTTCAGCAA+GGG	0.617085	8.3:+28537524	None:intergenic
GTAATGGACCGTATCCAGCC+TGG	0.617163	8.3:-28537255	MS.gene017586:CDS
AATAGGCAGCCTGAATCTCA+CGG	0.617647	8.3:+28537430	None:intergenic
GCAGGTAGTCCAAGCACCAA+AGG	0.618179	8.3:+28536538	None:intergenic
TCATCATTTGCTGCTGATGG+GGG	0.624594	8.3:-28536415	MS.gene017586:intron
CCAGCAGGAACATAGTGGCT+CGG	0.625474	8.3:+28536784	None:intergenic
GGCCGAAACTCTCCTCCTAG+TGG	0.633512	8.3:-28536748	MS.gene017586:CDS
ACAGCATTGACAGCACCACT+AGG	0.637601	8.3:+28536733	None:intergenic
AGACACCAGCAGGAACATAG+TGG	0.640185	8.3:+28536779	None:intergenic
GAGGCCAGCGATATTGAGGT+GGG	0.647056	8.3:+28537548	None:intergenic
ACGGACCGTCATCAAGAATG+CGG	0.654680	8.3:+28537301	None:intergenic
GCATTGACAGCACCACTAGG+AGG	0.655539	8.3:+28536736	None:intergenic
GTAGTCCAAGCACCAAAGGG+CGG	0.668886	8.3:+28536542	None:intergenic
TGGAAATTATCGTACAGCCA+CGG	0.685964	8.3:+28537282	None:intergenic
GGTTCAAGTCGACAGAAGTG+GGG	0.688223	8.3:+28537226	None:intergenic
AGTGGATGTTTCAGACTCGG+TGG	0.691705	8.3:+28537491	None:intergenic
TTAATGTTGCTCCCACACCA+CGG	0.702566	8.3:-28535578	MS.gene017586:CDS
GTTTCCAATTACCAACTCTG+AGG	0.709389	8.3:-28536509	MS.gene017586:CDS
TCTCACGGTCAGCTAAGACA+CGG	0.715862	8.3:+28537445	None:intergenic
AAGCGATGTTTAGATACACA+AGG	0.719194	8.3:+28535555	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	CAATAATTTCAACAGTAATA+TGG	+	chr8.3:28536951-28536970	None:intergenic	20.0%
!!!	ACTTTTAAATATGAGAAACT+TGG	-	chr8.3:28535969-28535988	MS.gene017586:intron	20.0%
!!!	GAAGTTCTTCAATTTTATTT+CGG	+	chr8.3:28536813-28536832	None:intergenic	20.0%
!	AAAGTTTCAACAATAGCATT+CGG	+	chr8.3:28537321-28537340	None:intergenic	25.0%
!	ATAGCCTCATAATAATATCA+TGG	+	chr8.3:28537500-28537519	None:intergenic	25.0%
!	CAATTATCTAACCAATTTAC+AGG	-	chr8.3:28537057-28537076	MS.gene017586:CDS	25.0%
!!!	ACTTTCCATTCTTTTTTCAA+AGG	+	chr8.3:28535918-28535937	None:intergenic	25.0%
!!!	GAAATTATTGATAGTGTTCT+TGG	-	chr8.3:28536961-28536980	MS.gene017586:CDS	25.0%
	AAACCATTAATAAGCCATGT+TGG	+	chr8.3:28537153-28537172	None:intergenic	30.0%
	ACATATTATTTGCAAGAGCA+CGG	+	chr8.3:28537284-28537303	None:intergenic	30.0%
	AGGTACTATGAATAGTGTTA+CGG	+	chr8.3:28535860-28535879	None:intergenic	30.0%
	ATGAAATTTCACCATTCTGA+AGG	+	chr8.3:28536523-28536542	None:intergenic	30.0%
	ATTAGGATAGCCATAATAAC+CGG	+	chr8.3:28537001-28537020	None:intergenic	30.0%
	ATTGGTTAGATAATTGTCGA+AGG	+	chr8.3:28537053-28537072	None:intergenic	30.0%
	CAACCATTTATTATTAGCAG+CGG	+	chr8.3:28536126-28536145	None:intergenic	30.0%
	CCTCATAATAATATCATGGT+CGG	+	chr8.3:28537496-28537515	None:intergenic	30.0%
	CGATAATTTCCATATCGTAA+TGG	-	chr8.3:28535683-28535702	MS.gene017586:intron	30.0%
	CTCATAATAATATCATGGTC+GGG	+	chr8.3:28537495-28537514	None:intergenic	30.0%
	GGAGCAACATTAACATAAAT+AGG	+	chr8.3:28537369-28537388	None:intergenic	30.0%
	GGTGAAATTGAAACAGAAAA+CGG	+	chr8.3:28535617-28535636	None:intergenic	30.0%
	GTTTCAATTTCACCACAAAA+TGG	-	chr8.3:28535623-28535642	MS.gene017586:intron	30.0%
	TCACTTAGGAATAAGATATC+CGG	+	chr8.3:28536778-28536797	None:intergenic	30.0%
	TGAAATTTCACCATTCTGAA+GGG	+	chr8.3:28536522-28536541	None:intergenic	30.0%
	TGCTATTGTTGAAACTTTAG+TGG	-	chr8.3:28537323-28537342	MS.gene017586:CDS	30.0%
	TTTACTTGGTTCAAAAGTCT+TGG	-	chr8.3:28537228-28537247	MS.gene017586:CDS	30.0%
!	ACGGTCTAATTTTTCTTTGA+CGG	+	chr8.3:28537265-28537284	None:intergenic	30.0%
!	AGACTTTTGAACCAAGTAAA+CGG	+	chr8.3:28537228-28537247	None:intergenic	30.0%
!	ATAGGAACAAATGTTAGTCT+TGG	-	chr8.3:28536578-28536597	MS.gene017586:CDS	30.0%
!	GTTTGATGACACTAATGTAA+TGG	-	chr8.3:28536637-28536656	MS.gene017586:CDS	30.0%
!!	TAGGAACAAATGTTAGTCTT+GGG	-	chr8.3:28536579-28536598	MS.gene017586:CDS	30.0%
!!!	AACTTGGAGTTTTTTGTTTC+AGG	-	chr8.3:28535985-28536004	MS.gene017586:intron	30.0%
	AAATCTCCGAAAATACACCA+GGG	+	chr8.3:28537084-28537103	None:intergenic	35.0%
	AACAAGAAGGTCATGTTTGA+GGG	+	chr8.3:28536291-28536310	None:intergenic	35.0%
	AAGCGATGTTTAGATACACA+AGG	+	chr8.3:28537402-28537421	None:intergenic	35.0%
	AATAGTGTTACGGACATCAT+AGG	+	chr8.3:28535850-28535869	None:intergenic	35.0%
	ACATTAGTGTCATCAAACAG+AGG	+	chr8.3:28536635-28536654	None:intergenic	35.0%
	ACTTGAACCATTTGGACATA+TGG	-	chr8.3:28535742-28535761	MS.gene017586:intron	35.0%
	AGACCAACATGGCTTATTAA+TGG	-	chr8.3:28537147-28537166	MS.gene017586:CDS	35.0%
	ATCCTTGATCACTGTTTGAT+TGG	-	chr8.3:28535486-28535505	MS.gene017586:intron	35.0%
	ATTCAACCAAATGTTGGTCA+AGG	-	chr8.3:28535795-28535814	MS.gene017586:intron	35.0%
	ATTTGGTTGAATGCAACCAA+AGG	+	chr8.3:28535787-28535806	None:intergenic	35.0%
	CATAAGCACCATTACTAACT+CGG	+	chr8.3:28535941-28535960	None:intergenic	35.0%
	CCGACCATGATATTATTATG+AGG	-	chr8.3:28537493-28537512	MS.gene017586:CDS	35.0%
	CTGAATCAATAACAGCTTGA+CGG	+	chr8.3:28537176-28537195	None:intergenic	35.0%
	CTTGAACCATTTGGACATAT+GGG	-	chr8.3:28535743-28535762	MS.gene017586:intron	35.0%
	GGTTATTATGGCTATCCTAA+TGG	-	chr8.3:28537000-28537019	MS.gene017586:CDS	35.0%
	GTGCTCTTGCAAATAATATG+TGG	-	chr8.3:28537283-28537302	MS.gene017586:CDS	35.0%
	GTGTATCTAAACATCGCTTT+AGG	-	chr8.3:28537403-28537422	MS.gene017586:CDS	35.0%
	GTTCCGCTGCTAATAATAAA+TGG	-	chr8.3:28536120-28536139	MS.gene017586:intron	35.0%
	TAAACGGATAACCTACAGTT+GGG	+	chr8.3:28537212-28537231	None:intergenic	35.0%
	TATTATGGCTATCCTAATGG+AGG	-	chr8.3:28537003-28537022	MS.gene017586:CDS	35.0%
	TCTTGATGCTAGCGAAAAAA+GGG	-	chr8.3:28537113-28537132	MS.gene017586:CDS	35.0%
	TTAAGATCAGGAATTGTGCT+CGG	+	chr8.3:28536733-28536752	None:intergenic	35.0%
	TTCTTGATGCTAGCGAAAAA+AGG	-	chr8.3:28537112-28537131	MS.gene017586:CDS	35.0%
	TTGTTACCTTCTTCTTCTAC+AGG	-	chr8.3:28536362-28536381	MS.gene017586:intron	35.0%
	TTTGGTTGAATGCAACCAAA+GGG	+	chr8.3:28535786-28535805	None:intergenic	35.0%
!	ATTTCATCATTTGCTGCTGA+TGG	-	chr8.3:28536536-28536555	MS.gene017586:CDS	35.0%
!	GCTTTAGGTATGAAAATGCA+TGG	-	chr8.3:28537418-28537437	MS.gene017586:CDS	35.0%
!	TTTCATCATTTGCTGCTGAT+GGG	-	chr8.3:28536537-28536556	MS.gene017586:CDS	35.0%
!!	AGGAACAAATGTTAGTCTTG+GGG	-	chr8.3:28536580-28536599	MS.gene017586:CDS	35.0%
!!	TCAGAGTTGGTAATTGGAAA+CGG	+	chr8.3:28536446-28536465	None:intergenic	35.0%
!!	TTGATAGTGTTCTTGGTGTA+TGG	-	chr8.3:28536968-28536987	MS.gene017586:CDS	35.0%
!!!	CCATTCTTTTTTCAAAGGAG+CGG	+	chr8.3:28535913-28535932	None:intergenic	35.0%
!!!	GAAGAACTTCGTTTTTTGCT+TGG	-	chr8.3:28536823-28536842	MS.gene017586:CDS	35.0%
	AACAGTGTCAAAGCGAAACA+TGG	-	chr8.3:28536317-28536336	MS.gene017586:intron	40.0%
	AAGGAGCGGTGACATTAATA+CGG	+	chr8.3:28535899-28535918	None:intergenic	40.0%
	AATGCATGGTATCTTGAACC+AGG	-	chr8.3:28537432-28537451	MS.gene017586:CDS	40.0%
	ACACACTGACAAAGTTTGTC+CGG	-	chr8.3:28536022-28536041	MS.gene017586:intron	40.0%
	ATAGGATAGTGATCAGAAGC+AGG	+	chr8.3:28537351-28537370	None:intergenic	40.0%
	CAAACAGTGATCAAGGATAG+TGG	+	chr8.3:28535484-28535503	None:intergenic	40.0%
	CAACAAGAAGGTCATGTTTG+AGG	+	chr8.3:28536292-28536311	None:intergenic	40.0%
	CTGAACTGAAGAAGAGAAAG+AGG	-	chr8.3:28536489-28536508	MS.gene017586:CDS	40.0%
	CTGATCTTAAGTACCTAGTC+CGG	-	chr8.3:28536743-28536762	MS.gene017586:CDS	40.0%
	CTGTAGGTTATCCGTTTACT+TGG	-	chr8.3:28537214-28537233	MS.gene017586:CDS	40.0%
	CTTGATGCTAGCGAAAAAAG+GGG	-	chr8.3:28537114-28537133	MS.gene017586:CDS	40.0%
	GAAATCTCCGAAAATACACC+AGG	+	chr8.3:28537085-28537104	None:intergenic	40.0%
	GATGACACTAATGTAATGGC+AGG	-	chr8.3:28536641-28536660	MS.gene017586:CDS	40.0%
	GATTGTCAACAGCAAGCATA+CGG	+	chr8.3:28536259-28536278	None:intergenic	40.0%
	GGTGAGGGAAACTTTGTAAT+AGG	-	chr8.3:28536560-28536579	MS.gene017586:CDS	40.0%
	GGTTGCATTCAACCAAATGT+TGG	-	chr8.3:28535789-28535808	MS.gene017586:intron	40.0%
	GTAAACGGATAACCTACAGT+TGG	+	chr8.3:28537213-28537232	None:intergenic	40.0%
	GTAACAAGTTCCTTAAAGCC+TGG	+	chr8.3:28537453-28537472	None:intergenic	40.0%
	GTATCTTGAACCAGGCTTTA+AGG	-	chr8.3:28537440-28537459	MS.gene017586:CDS	40.0%
	GTTTCCAATTACCAACTCTG+AGG	-	chr8.3:28536445-28536464	MS.gene017586:CDS	40.0%
	TAAGATCAGGAATTGTGCTC+GGG	+	chr8.3:28536732-28536751	None:intergenic	40.0%
	TATTAGCAGCGGAACCAATA+CGG	+	chr8.3:28536115-28536134	None:intergenic	40.0%
	TCAGCTTCAAACTTACTTGC+TGG	-	chr8.3:28536389-28536408	MS.gene017586:intron	40.0%
	TGGAAAGTCCGAGTTAGTAA+TGG	-	chr8.3:28535930-28535949	MS.gene017586:intron	40.0%
	TGGAAATTATCGTACAGCCA+CGG	+	chr8.3:28535675-28535694	None:intergenic	40.0%
	TTGATGCTAGCGAAAAAAGG+GGG	-	chr8.3:28537115-28537134	MS.gene017586:CDS	40.0%
	TTGTCGAAGGAAATTCCAAG+CGG	+	chr8.3:28537040-28537059	None:intergenic	40.0%
!	AATAAGCCATGTTGGTCTAG+TGG	+	chr8.3:28537145-28537164	None:intergenic	40.0%
!	AATTTTTCTTTGACGGCACG+TGG	+	chr8.3:28537258-28537277	None:intergenic	40.0%
!	AGCAAGTAAGTTTGAAGCTG+AGG	+	chr8.3:28536390-28536409	None:intergenic	40.0%
!	CCGCTCCTTTGAAAAAAGAA+TGG	-	chr8.3:28535910-28535929	MS.gene017586:intron	40.0%
!	TGGAGACTTACCGGTTATTA+TGG	-	chr8.3:28536988-28537007	MS.gene017586:CDS	40.0%
!	TTCATCATTTGCTGCTGATG+GGG	-	chr8.3:28536538-28536557	MS.gene017586:CDS	40.0%
!!	AACAAATGTTAGTCTTGGGG+GGG	-	chr8.3:28536583-28536602	MS.gene017586:CDS	40.0%
!!	AGGAACTTGTTACTAACTCG+TGG	-	chr8.3:28537460-28537479	MS.gene017586:CDS	40.0%
!!	CGTCAAGCTGTTATTGATTC+AGG	-	chr8.3:28537174-28537193	MS.gene017586:CDS	40.0%
!!	GAACAAATGTTAGTCTTGGG+GGG	-	chr8.3:28536582-28536601	MS.gene017586:CDS	40.0%
!!	GATAGTGGATGTTTCAGACT+CGG	+	chr8.3:28535469-28535488	None:intergenic	40.0%
!!	GGAACAAATGTTAGTCTTGG+GGG	-	chr8.3:28536581-28536600	MS.gene017586:CDS	40.0%
!!	TTCTGTCGACTTGAACCATT+TGG	-	chr8.3:28535734-28535753	MS.gene017586:intron	40.0%
!!!	GGAGTTTTTTGTTTCAGGTG+TGG	-	chr8.3:28535990-28536009	MS.gene017586:intron	40.0%
	AATAGGCAGCCTGAATCTCA+CGG	+	chr8.3:28535527-28535546	None:intergenic	45.0%
	ACGGACTTTCAGACGCATAT+AGG	+	chr8.3:28535880-28535899	None:intergenic	45.0%
	AGATATCCGGATTGAAGTGC+CGG	+	chr8.3:28536765-28536784	None:intergenic	45.0%
	AGCCATACGCTCACTGAAAT+AGG	+	chr8.3:28535544-28535563	None:intergenic	45.0%
	ATCTTGAGACTCCTCAGAGT+TGG	+	chr8.3:28536459-28536478	None:intergenic	45.0%
	ATGGTTCAAGTCGACAGAAG+TGG	+	chr8.3:28535733-28535752	None:intergenic	45.0%
	ATTAGCAGCGGAACCAATAC+GGG	+	chr8.3:28536114-28536133	None:intergenic	45.0%
	CAAGTGTATCTGACAAGCAC+CGG	+	chr8.3:28536345-28536364	None:intergenic	45.0%
	CCAAATGTTGGTCAAGGCAT+AGG	-	chr8.3:28535801-28535820	MS.gene017586:intron	45.0%
	CCTATGCCTTGACCAACATT+TGG	+	chr8.3:28535804-28535823	None:intergenic	45.0%
	CTAACCAATTTACAGGTCCC+TGG	-	chr8.3:28537064-28537083	MS.gene017586:CDS	45.0%
	CTAGACTCATTTACCTAGGC+AGG	+	chr8.3:28536681-28536700	None:intergenic	45.0%
	GAAGAAGAGAAAGAGGATGC+TGG	-	chr8.3:28536496-28536515	MS.gene017586:CDS	45.0%
	GCCACTAGACTCATTTACCT+AGG	+	chr8.3:28536685-28536704	None:intergenic	45.0%
	GCCTAGGTAAATGAGTCTAG+TGG	-	chr8.3:28536681-28536700	MS.gene017586:CDS	45.0%
	GGATACGGTCCATTACGATA+TGG	+	chr8.3:28535695-28535714	None:intergenic	45.0%
	GGCCAATCAAACAGTGATCA+AGG	+	chr8.3:28535491-28535510	None:intergenic	45.0%
	GGTAAATGAGTCTAGTGGCA+TGG	-	chr8.3:28536686-28536705	MS.gene017586:CDS	45.0%
	GGTTAAACCCTCTTCAGCAA+GGG	+	chr8.3:28535433-28535452	None:intergenic	45.0%
	GTAATTGGAAACGGTTCAGC+AGG	+	chr8.3:28536437-28536456	None:intergenic	45.0%
	GTCTAGTGGCATGGAATTGT+CGG	-	chr8.3:28536695-28536714	MS.gene017586:CDS	45.0%
	TACACCAGGGACCTGTAAAT+TGG	+	chr8.3:28537071-28537090	None:intergenic	45.0%
	TCTAGTGGCATGGAATTGTC+GGG	-	chr8.3:28536696-28536715	MS.gene017586:CDS	45.0%
	TGCCTATTTCAGTGAGCGTA+TGG	-	chr8.3:28535539-28535558	MS.gene017586:intron	45.0%
	TGGTTCAAGTCGACAGAAGT+GGG	+	chr8.3:28535732-28535751	None:intergenic	45.0%
	TGTTTAGATACACAAGGCCG+TGG	+	chr8.3:28537396-28537415	None:intergenic	45.0%
	TTAATGTTGCTCCCACACCA+CGG	-	chr8.3:28537376-28537395	MS.gene017586:CDS	45.0%
	TTGTCTGATGTCCCAACTGT+AGG	-	chr8.3:28537198-28537217	MS.gene017586:CDS	45.0%
!	ACAGGTCCCTGGTGTATTTT+CGG	-	chr8.3:28537075-28537094	MS.gene017586:CDS	45.0%
!	ACTTGTTACTAACTCGTGGC+AGG	-	chr8.3:28537464-28537483	MS.gene017586:CDS	45.0%
!	CACTGTTTTGCGCAACAAGA+AGG	+	chr8.3:28536304-28536323	None:intergenic	45.0%
!	CGGACTAGGTACTTAAGATC+AGG	+	chr8.3:28536745-28536764	None:intergenic	45.0%
!	CTTGGTGTATGGAGACTTAC+CGG	-	chr8.3:28536979-28536998	MS.gene017586:CDS	45.0%
!	GAGGTACCTGTAGAAGAAGA+AGG	+	chr8.3:28536371-28536390	None:intergenic	45.0%
!	TCATCATTTGCTGCTGATGG+GGG	-	chr8.3:28536539-28536558	MS.gene017586:CDS	45.0%
!!	GACTCCTCAGAGTTGGTAAT+TGG	+	chr8.3:28536452-28536471	None:intergenic	45.0%
!!	TGATGATGGTGTCCGCAATT+GGG	-	chr8.3:28536064-28536083	MS.gene017586:intron	45.0%
!!	TGTTTCAGGTGTGGTTTGCT+TGG	-	chr8.3:28535999-28536018	MS.gene017586:intron	45.0%
	AAGCCTAATGCTGCCCGTAT+TGG	-	chr8.3:28536098-28536117	MS.gene017586:intron	50.0%
	AATGGCAGGTCCTGATGATC+AGG	-	chr8.3:28536655-28536674	MS.gene017586:CDS	50.0%
	ACGGACCGTCATCAAGAATG+CGG	+	chr8.3:28535656-28535675	None:intergenic	50.0%
	AGACACCAGCAGGAACATAG+TGG	+	chr8.3:28536178-28536197	None:intergenic	50.0%
	AGTGGATGTTTCAGACTCGG+TGG	+	chr8.3:28535466-28535485	None:intergenic	50.0%
	CAAGGCATAGGCAGCTTTCT+AGG	-	chr8.3:28535813-28535832	MS.gene017586:intron	50.0%
	CACCACTAGGAGGAGAGTTT+CGG	+	chr8.3:28536211-28536230	None:intergenic	50.0%
	CAGCATTAGGCTTCAGGTCA+GGG	+	chr8.3:28536091-28536110	None:intergenic	50.0%
	CAGCGATATTGAGGTGGGAA+GGG	+	chr8.3:28535404-28535423	None:intergenic	50.0%
	CAGGTAGTCCAAGCACCAAA+GGG	+	chr8.3:28536418-28536437	None:intergenic	50.0%
	CCATTTGGACATATGGGTCC+AGG	-	chr8.3:28535749-28535768	MS.gene017586:intron	50.0%
	CCTGGACCCATATGTCCAAA+TGG	+	chr8.3:28535752-28535771	None:intergenic	50.0%
	CTAGTGGCATGGAATTGTCG+GGG	-	chr8.3:28536697-28536716	MS.gene017586:CDS	50.0%
	CTTAGCTGACCGTGAGATTC+AGG	-	chr8.3:28535515-28535534	MS.gene017586:intron	50.0%
	GAACCAATACGGGCAGCATT+AGG	+	chr8.3:28536104-28536123	None:intergenic	50.0%
	GACATATGGGTCCAGGTTCA+CGG	-	chr8.3:28535756-28535775	MS.gene017586:intron	50.0%
	GAGGAGTCTCAAGATGCTAC+TGG	-	chr8.3:28536464-28536483	MS.gene017586:CDS	50.0%
	GCATGGAATTGTCGGGGTAT+AGG	-	chr8.3:28536703-28536722	MS.gene017586:CDS	50.0%
	GCTGTTCTACGACCTCCATT+AGG	+	chr8.3:28537018-28537037	None:intergenic	50.0%
	GGGTTAAACCCTCTTCAGCA+AGG	+	chr8.3:28535434-28535453	None:intergenic	50.0%
	GGTGAGCTAGAGTCTCACTT+AGG	+	chr8.3:28536792-28536811	None:intergenic	50.0%
	GGTTCAAGTCGACAGAAGTG+GGG	+	chr8.3:28535731-28535750	None:intergenic	50.0%
	GTCAAAGCGAAACATGGCTC+CGG	-	chr8.3:28536323-28536342	MS.gene017586:intron	50.0%
	TCGGCACAGCAGTAGGAATT+GGG	+	chr8.3:28536154-28536173	None:intergenic	50.0%
	TCTCACGGTCAGCTAAGACA+CGG	+	chr8.3:28535512-28535531	None:intergenic	50.0%
	TTCGAGCTGGAGTCTGATGA+TGG	-	chr8.3:28536050-28536069	MS.gene017586:intron	50.0%
!	GATGATGGTGTCCGCAATTG+GGG	-	chr8.3:28536065-28536084	MS.gene017586:intron	50.0%
!	TGTTTTCCTGTAGACCGCAC+CGG	-	chr8.3:28536856-28536875	MS.gene017586:CDS	50.0%
!!	ACAGCATTGACAGCACCACT+AGG	+	chr8.3:28536224-28536243	None:intergenic	50.0%
!!	AGACTCGGTGGCTAGGTTAA+GGG	+	chr8.3:28535454-28535473	None:intergenic	50.0%
!!	ATGTTTCAGACTCGGTGGCT+AGG	+	chr8.3:28535461-28535480	None:intergenic	50.0%
!!	CTGATGATGGTGTCCGCAAT+TGG	-	chr8.3:28536063-28536082	MS.gene017586:intron	50.0%
!!!	GCGGGGGTCTAGCTTTATTT+TGG	-	chr8.3:28536884-28536903	MS.gene017586:CDS	50.0%
	AACCCTCTTCAGCAAGGGTG+AGG	+	chr8.3:28535428-28535447	None:intergenic	55.0%
	ACAGAAGTGGGGACAACACC+AGG	+	chr8.3:28535720-28535739	None:intergenic	55.0%
	ACGATCGAAACACGCTTGCC+TGG	+	chr8.3:28535579-28535598	None:intergenic	55.0%
	AGATACACAAGGCCGTGGTG+TGG	+	chr8.3:28537391-28537410	None:intergenic	55.0%
	AGGATGCTGGCCCTTCAGAA+TGG	-	chr8.3:28536509-28536528	MS.gene017586:CDS	55.0%
	CAAAGGGAAGACCGTGAACC+TGG	+	chr8.3:28535770-28535789	None:intergenic	55.0%
	CAGGTTCACGGTCTTCCCTT+TGG	-	chr8.3:28535768-28535787	MS.gene017586:intron	55.0%
	CCAGCAGGAACATAGTGGCT+CGG	+	chr8.3:28536173-28536192	None:intergenic	55.0%
	CCAGCATGAAAGACACCAGC+AGG	+	chr8.3:28536188-28536207	None:intergenic	55.0%
	CCAGCGATATTGAGGTGGGA+AGG	+	chr8.3:28535405-28535424	None:intergenic	55.0%
	CCTAGTCCGGCACTTCAATC+CGG	-	chr8.3:28536756-28536775	MS.gene017586:CDS	55.0%
	CCTGCTGGTGTCTTTCATGC+TGG	-	chr8.3:28536185-28536204	MS.gene017586:intron	55.0%
	CCTTCCCACCTCAATATCGC+TGG	-	chr8.3:28535402-28535421	MS.gene017586:CDS	55.0%
	CGGACCGTCATCAAGAATGC+GGG	+	chr8.3:28535655-28535674	None:intergenic	55.0%
	CTCGGCACAGCAGTAGGAAT+TGG	+	chr8.3:28536155-28536174	None:intergenic	55.0%
	GACTCCAGCTCGAACAACTC+CGG	+	chr8.3:28536044-28536063	None:intergenic	55.0%
	GAGGCCAGCGATATTGAGGT+GGG	+	chr8.3:28535409-28535428	None:intergenic	55.0%
	GATACACAAGGCCGTGGTGT+GGG	+	chr8.3:28537390-28537409	None:intergenic	55.0%
	GCAGCATTAGGCTTCAGGTC+AGG	+	chr8.3:28536092-28536111	None:intergenic	55.0%
	GCAGGTAGTCCAAGCACCAA+AGG	+	chr8.3:28536419-28536438	None:intergenic	55.0%
	GCGCAGACGACATGTCAGTT+TGG	-	chr8.3:28537526-28537545	MS.gene017586:CDS	55.0%
	GCTGCCCGCATTCTTGATGA+CGG	-	chr8.3:28535648-28535667	MS.gene017586:intron	55.0%
	GGACAACACCAGGCTGGATA+CGG	+	chr8.3:28535710-28535729	None:intergenic	55.0%
	GTAGTCCAAGCACCAAAGGG+CGG	+	chr8.3:28536415-28536434	None:intergenic	55.0%
	TACGGGCAGCATTAGGCTTC+AGG	+	chr8.3:28536097-28536116	None:intergenic	55.0%
	TGAGGCCAGCGATATTGAGG+TGG	+	chr8.3:28535410-28535429	None:intergenic	55.0%
	TTGTCCGGAGTTGTTCGAGC+TGG	-	chr8.3:28536037-28536056	MS.gene017586:intron	55.0%
	TTTGCTGCTGATGGGGGTGA+GGG	-	chr8.3:28536545-28536564	MS.gene017586:CDS	55.0%
!	ATTTGCTGCTGATGGGGGTG+AGG	-	chr8.3:28536544-28536563	MS.gene017586:CDS	55.0%
!	GCATTGACAGCACCACTAGG+AGG	+	chr8.3:28536221-28536240	None:intergenic	55.0%
!	TGCGGGCAGCATCCATTTTG+TGG	+	chr8.3:28535638-28535657	None:intergenic	55.0%
!!	CAGACTCGGTGGCTAGGTTA+AGG	+	chr8.3:28535455-28535474	None:intergenic	55.0%
!!	GCATTCTTGATGACGGTCCG+TGG	-	chr8.3:28535655-28535674	MS.gene017586:intron	55.0%
!!	GTAATGGACCGTATCCAGCC+TGG	-	chr8.3:28535699-28535718	MS.gene017586:intron	55.0%
	AAGTGGGGACAACACCAGGC+TGG	+	chr8.3:28535716-28535735	None:intergenic	60.0%
	ATGGCTCGTGCTTGGAAGCC+AGG	-	chr8.3:28535558-28535577	MS.gene017586:CDS	60.0%
	CCGAGCCACTATGTTCCTGC+TGG	-	chr8.3:28536170-28536189	MS.gene017586:intron	60.0%
	CCTAGGCAGGCCTGATCATC+AGG	+	chr8.3:28536668-28536687	None:intergenic	60.0%
	CCTGATGATCAGGCCTGCCT+AGG	-	chr8.3:28536665-28536684	MS.gene017586:CDS	60.0%
	GAGGTCGTAGAACAGCCGCT+TGG	-	chr8.3:28537022-28537041	MS.gene017586:CDS	60.0%
	GGCCGAAACTCTCCTCCTAG+TGG	-	chr8.3:28536206-28536225	MS.gene017586:intron	60.0%
	GGCCTCACCCTTGCTGAAGA+GGG	-	chr8.3:28535423-28535442	MS.gene017586:CDS	60.0%
	GGCGCACCACTAGACCAACA+TGG	-	chr8.3:28537136-28537155	MS.gene017586:CDS	60.0%
	GGGTGAGGCCAGCGATATTG+AGG	+	chr8.3:28535413-28535432	None:intergenic	60.0%
	GTGAGCGTATGGCTCGTGCT+TGG	-	chr8.3:28535550-28535569	MS.gene017586:intron	60.0%
	TCAGGTCAGGGCCCCAATTG+CGG	+	chr8.3:28536079-28536098	None:intergenic	60.0%
	TGGCCTCACCCTTGCTGAAG+AGG	-	chr8.3:28535422-28535441	MS.gene017586:CDS	60.0%
!	CTTACTTGCTGGCCGCCCTT+TGG	-	chr8.3:28536400-28536419	MS.gene017586:intron	60.0%
!	TAGTGGCTCGGCACAGCAGT+AGG	+	chr8.3:28536161-28536180	None:intergenic	60.0%
!!	CCGGATTGAAGTGCCGGACT+AGG	+	chr8.3:28536759-28536778	None:intergenic	60.0%
	AGCTAGACCCCCGCCTCTAC+CGG	+	chr8.3:28536878-28536897	None:intergenic	65.0%
	CCTCTACCGGTGCGGTCTAC+AGG	+	chr8.3:28536865-28536884	None:intergenic	65.0%
!	CCTGTAGACCGCACCGGTAG+AGG	-	chr8.3:28536862-28536881	MS.gene017586:CDS	65.0%
!	GTAGACCGCACCGGTAGAGG+CGG	-	chr8.3:28536865-28536884	MS.gene017586:CDS	65.0%
!	TAGACCGCACCGGTAGAGGC+GGG	-	chr8.3:28536866-28536885	MS.gene017586:CDS	65.0%
!	AGACCGCACCGGTAGAGGCG+GGG	-	chr8.3:28536867-28536886	MS.gene017586:CDS	70.0%
!!	GCTGGCCGCCCTTTGGTGCT+TGG	-	chr8.3:28536407-28536426	MS.gene017586:intron	70.0%
	GACCCCCGCCTCTACCGGTG+CGG	+	chr8.3:28536873-28536892	None:intergenic	75.0%
!	GACCGCACCGGTAGAGGCGG+GGG	-	chr8.3:28536868-28536887	MS.gene017586:CDS	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr8.3	gene	28535396	28537580	28535396	ID=MS.gene017586
chr8.3	mRNA	28535396	28537580	28535396	ID=MS.gene017586.t1;Parent=MS.gene017586
chr8.3	exon	28536416	28537580	28536416	ID=MS.gene017586.t1.exon1;Parent=MS.gene017586.t1
chr8.3	CDS	28536416	28537580	28536416	ID=cds.MS.gene017586.t1;Parent=MS.gene017586.t1
chr8.3	exon	28535552	28535633	28535552	ID=MS.gene017586.t1.exon2;Parent=MS.gene017586.t1
chr8.3	CDS	28535552	28535633	28535552	ID=cds.MS.gene017586.t1;Parent=MS.gene017586.t1
chr8.3	exon	28535396	28535444	28535396	ID=MS.gene017586.t1.exon3;Parent=MS.gene017586.t1
chr8.3	CDS	28535396	28535444	28535396	ID=cds.MS.gene017586.t1;Parent=MS.gene017586.t1

Gene Sequence

>MS.gene017586

ATGGACCCTTCCCACCTCAATATCGCTGGCCTCACCCTTGCTGAAGAGGGTTTAACCCTTAACCTAGCCACCGAGTCTGAAACATCCACTATCCTTGATCACTGTTTGATTGGCCGTGTCTTAGCTGACCGTGAGATTCAGGCTGCCTATTTCAGTGAGCGTATGGCTCGTGCTTGGAAGCCAGGCAAGCGTGTTTCGATCGTCAAAACTGATAGTGACCGTTTTCTGTTTCAATTTCACCACAAAATGGATGCTGCCCGCATTCTTGATGACGGTCCGTGGCTGTACGATAATTTCCATATCGTAATGGACCGTATCCAGCCTGGTGTTGTCCCCACTTCTGTCGACTTGAACCATTTGGACATATGGGTCCAGGTTCACGGTCTTCCCTTTGGTTGCATTCAACCAAATGTTGGTCAAGGCATAGGCAGCTTTCTAGGTGAACTCAAATCCTATGATGTCCGTAACACTATTCATAGTACCTATATGCGTCTGAAAGTCCGTATTAATGTCACCGCTCCTTTGAAAAAAGAATGGAAAGTCCGAGTTAGTAATGGTGCTTATGTTACAGTAACTTTTAAATATGAGAAACTTGGAGTTTTTTGTTTCAGGTGTGGTTTGCTTGGACACACTGACAAAGTTTGTCCGGAGTTGTTCGAGCTGGAGTCTGATGATGGTGTCCGCAATTGGGGCCCTGACCTGAAGCCTAATGCTGCCCGTATTGGTTCCGCTGCTAATAATAAATGGTTGCAAGACCCAATTCCTACTGCTGTGCCGAGCCACTATGTTCCTGCTGGTGTCTTTCATGCTGGCCGAAACTCTCCTCCTAGTGGTGCTGTCAATGCTGTAACTTTCAGTGACCGTATGCTTGCTGTTGACAATCAGATTTCTGCCCTCAAACATGACCTTCTTGTTGCGCAAAACAGTGTCAAAGCGAAACATGGCTCCGGTGCTTGTCAGATACACTTGTTACCTTCTTCTTCTACAGGTACCTCAGCTTCAAACTTACTTGCTGGCCGCCCTTTGGTGCTTGGACTACCTGCTGAACCGTTTCCAATTACCAACTCTGAGGAGTCTCAAGATGCTACTGGTTCTGAACTGAAGAAGAGAAAGAGGATGCTGGCCCTTCAGAATGGTGAAATTTCATCATTTGCTGCTGATGGGGGTGAGGGAAACTTTGTAATAGGAACAAATGTTAGTCTTGGGGGGGATGTTGATATGAATGTAATCTCTAATCCTCTGTTTGATGACACTAATGTAATGGCAGGTCCTGATGATCAGGCCTGCCTAGGTAAATGAGTCTAGTGGCATGGAATTGTCGGGGTATAGGTAGCCCGAGCACAATTCCTGATCTTAAGTACCTAGTCCGGCACTTCAATCCGGATATCTTATTCCTAAGTGAGACTCTAGCTCACCGAAATAAAATTGAAGAACTTCGTTTTTTGCTTGGTTATGACTGTTGTTTTCCTGTAGACCGCACCGGTAGAGGCGGGGGTCTAGCTTTATTTTGGCGTAACTCTTTACATTGCAAACTTGTTGATTTTTCTAGCAACCATATTACTGTTGAAATTATTGATAGTGTTCTTGGTGTATGGAGACTTACCGGTTATTATGGCTATCCTAATGGAGGTCGTAGAACAGCCGCTTGGAATTTCCTTCGACAATTATCTAACCAATTTACAGGTCCCTGGTGTATTTTCGGAGATTTCAACGATATTCTTGATGCTAGCGAAAAAAGGGGGCGCACCACTAGACCAACATGGCTTATTAATGGTTTCCGTCAAGCTGTTATTGATTCAGGTTTGTCTGATGTCCCAACTGTAGGTTATCCGTTTACTTGGTTCAAAAGTCTTGGTACTCCACGTGCCGTCAAAGAAAAATTAGACCGTGCTCTTGCAAATAATATGTGGTTTAATATTTTTCCGAATGCTATTGTTGAAACTTTAGTGGCACCTGCTTCTGATCACTATCCTATTTATGTTAATGTTGCTCCCACACCACGGCCTTGTGTATCTAAACATCGCTTTAGGTATGAAAATGCATGGTATCTTGAACCAGGCTTTAAGGAACTTGTTACTAACTCGTGGCAGGAAAATCCCGACCATGATATTATTATGAGGCTATCTGTATGCGCAGACGACATGTCAGTTTGGAGAAAATCTCATTGTCACAAACTAAAAATTGA

Protein sequence

>MS.gene017586.t1

MDPSHLNIAGLTLAEEGLTLNLATESETSTILDHCLIGRVLADREIQAAYFSERMARAWKPGKRVSIVKTDSDRFLFQFHHKMDAARILDDGPWLYDNFHIVMDRIQPGVVPTSVDLNHLDIWVQVHGLPFGCIQPNVGQGIGSFLGELKSYDVRNTIHSTYMRLKVRINVTAPLKKEWKVRVSNGAYVTVTFKYEKLGVFCFRCGLLGHTDKVCPELFELESDDGVRNWGPDLKPNAARIGSAANNKWLQDPIPTAVPSHYVPAGVFHAGRNSPPSGAVNAVTFSDRMLAVDNQISALKHDLLVAQNSVKAKHGSGACQIHLLPSSSTGTSASNLLAGRPLVLGLPAEPFPITNSEESQDATGSELKKRKRMLALQNGEISSFAADGVAPASDHYPIYVNVAPTPRPCVSKHRFRRHVSLEKISLSQTKN