AlfalfaGEDB | MS.gene025873

M. sativa cultivar XinJiangDaYe / MS.gene025873

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025873.t1	AES70414.1	64	236	56	4	1	223	55	274	2.10E-71	279.3

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025873.t1	G7J017	64.0	236	56	4	1	223	55	274	1.5e-71	279.3

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene025873	MS.gene21066	PPI
MS.gene025873	MS.gene009213	PPI
MS.gene025873	MS.gene013890	PPI
MS.gene038676	MS.gene025873	PPI
MS.gene025873	MS.gene98127	PPI
MS.gene025873	MS.gene003264	PPI
MS.gene025873	MS.gene054652	PPI
MS.gene025873	MS.gene000634	PPI
MS.gene025873	MS.gene86849	PPI
MS.gene025873	MS.gene42903	PPI
MS.gene025873	MS.gene80362	PPI
MS.gene025873	MS.gene49057	PPI
MS.gene025873	MS.gene89815	PPI
MS.gene025873	MS.gene018474	PPI
MS.gene025873	MS.gene048088	PPI
MS.gene025873	MS.gene86868	PPI
MS.gene025873	MS.gene058889	PPI
MS.gene025873	MS.gene034643	PPI
MS.gene025873	MS.gene60059	PPI
MS.gene025873	MS.gene050679	PPI
MS.gene42903	MS.gene025873	PPI
MS.gene025873	MS.gene012889	PPI
MS.gene025873	MS.gene34463	PPI
MS.gene025873	MS.gene038757	PPI
MS.gene025873	MS.gene015248	PPI
MS.gene025873	MS.gene030090	PPI
MS.gene025873	MS.gene61499	PPI
MS.gene042559	MS.gene025873	PPI
MS.gene025873	MS.gene51804	PPI
MS.gene22200	MS.gene025873	PPI
MS.gene025873	MS.gene013644	PPI
MS.gene025873	MS.gene05358	PPI
MS.gene025873	MS.gene061579	PPI
MS.gene34812	MS.gene025873	PPI
MS.gene063214	MS.gene025873	PPI
MS.gene025873	MS.gene025675	PPI
MS.gene025873	MS.gene69495	PPI
MS.gene035626	MS.gene025873	PPI
MS.gene025873	MS.gene04970	PPI
MS.gene067340	MS.gene025873	PPI
MS.gene025873	MS.gene71637	PPI
MS.gene025873	MS.gene26038	PPI
MS.gene025873	MS.gene43924	PPI
MS.gene69495	MS.gene025873	PPI
MS.gene025873	MS.gene022929	PPI
MS.gene025873	MS.gene85295	PPI
MS.gene025873	MS.gene26433	PPI
MS.gene025873	MS.gene007022	PPI
MS.gene05357	MS.gene025873	PPI
MS.gene025873	MS.gene69584	PPI
MS.gene025873	MS.gene52349	PPI
MS.gene025873	MS.gene021647	PPI
MS.gene025873	MS.gene047954	PPI
MS.gene025873	MS.gene30136	PPI
MS.gene025873	MS.gene65471	PPI
MS.gene025873	MS.gene071784	PPI
MS.gene025873	MS.gene048342	PPI
MS.gene025873	MS.gene069624	PPI
MS.gene025873	MS.gene060386	PPI
MS.gene025873	MS.gene83979	PPI
MS.gene021968	MS.gene025873	PPI
MS.gene025873	MS.gene073582	PPI
MS.gene025873	MS.gene63762	PPI
MS.gene025873	MS.gene016719	PPI
MS.gene025873	MS.gene048072	PPI
MS.gene025873	MS.gene028714	PPI
MS.gene025873	MS.gene88393	PPI
MS.gene025873	MS.gene042027	PPI
MS.gene025873	MS.gene048706	PPI
MS.gene025873	MS.gene69586	PPI
MS.gene025873	MS.gene45083	PPI
MS.gene025873	MS.gene049561	PPI
MS.gene025873	MS.gene008658	PPI
MS.gene025873	MS.gene47792	PPI
MS.gene025873	MS.gene63150	PPI
MS.gene025873	MS.gene03936	PPI
MS.gene025873	MS.gene43393	PPI
MS.gene025873	MS.gene000629	PPI
MS.gene025873	MS.gene072852	PPI
MS.gene025873	MS.gene34812	PPI
MS.gene025873	MS.gene08708	PPI
MS.gene025873	MS.gene06164	PPI
MS.gene025873	MS.gene50392	PPI
MS.gene025873	MS.gene86275	PPI
MS.gene025873	MS.gene22730	PPI
MS.gene025873	MS.gene017586	PPI
MS.gene025873	MS.gene038098	PPI
MS.gene025873	MS.gene037634	PPI
MS.gene025873	MS.gene057828	PPI
MS.gene025873	MS.gene071320	PPI
MS.gene025873	MS.gene08606	PPI
MS.gene025873	MS.gene012019	PPI
MS.gene025873	MS.gene46941	PPI
MS.gene025873	MS.gene048747	PPI
MS.gene025873	MS.gene000640	PPI
MS.gene025873	MS.gene000506	PPI
MS.gene025873	MS.gene061850	PPI
MS.gene025873	MS.gene98756	PPI
MS.gene025873	MS.gene39956	PPI
MS.gene025873	MS.gene028716	PPI
MS.gene025873	MS.gene74639	PPI
MS.gene96109	MS.gene025873	PPI
MS.gene025873	MS.gene071133	PPI
MS.gene025873	MS.gene64130	PPI
MS.gene025873	MS.gene60266	PPI
MS.gene025873	MS.gene98467	PPI
MS.gene025873	MS.gene024522	PPI
MS.gene025873	MS.gene54051	PPI
MS.gene025873	MS.gene22111	PPI
MS.gene025873	MS.gene049610	PPI
MS.gene025873	MS.gene010290	PPI
MS.gene025873	MS.gene93396	PPI
MS.gene025873	MS.gene37955	PPI
MS.gene025873	MS.gene95561	PPI
MS.gene94654	MS.gene025873	PPI
MS.gene025873	MS.gene92517	PPI
MS.gene025873	MS.gene34197	PPI
MS.gene025873	MS.gene36069	PPI
MS.gene025873	MS.gene045875	PPI
MS.gene025873	MS.gene010890	PPI
MS.gene025873	MS.gene84790	PPI
MS.gene025873	MS.gene71675	PPI
MS.gene025873	MS.gene055683	PPI
MS.gene025873	MS.gene05319	PPI
MS.gene025873	MS.gene039692	PPI
MS.gene025873	MS.gene032311	PPI
MS.gene025873	MS.gene002590	PPI
MS.gene025873	MS.gene41979	PPI
MS.gene96541	MS.gene025873	PPI
MS.gene025873	MS.gene008927	PPI
MS.gene025873	MS.gene060788	PPI
MS.gene025873	MS.gene98616	PPI
MS.gene025873	MS.gene23521	PPI
MS.gene025873	MS.gene41643	PPI
MS.gene025873	MS.gene77990	PPI
MS.gene025873	MS.gene65546	PPI
MS.gene30208	MS.gene025873	PPI
MS.gene025873	MS.gene062511	PPI
MS.gene025873	MS.gene008861	PPI
MS.gene025873	MS.gene065627	PPI
MS.gene025873	MS.gene027994	PPI
MS.gene025873	MS.gene059269	PPI
MS.gene025873	MS.gene96541	PPI
MS.gene05358	MS.gene025873	PPI
MS.gene025873	MS.gene045614	PPI
MS.gene025873	MS.gene064966	PPI
MS.gene95561	MS.gene025873	PPI
MS.gene025873	MS.gene036423	PPI
MS.gene025873	MS.gene76420	PPI
MS.gene065627	MS.gene025873	PPI
MS.gene025873	MS.gene46353	PPI
MS.gene57019	MS.gene025873	PPI
MS.gene025873	MS.gene51799	PPI
MS.gene025873	MS.gene98471	PPI
MS.gene025873	MS.gene062067	PPI
MS.gene025873	MS.gene61651	PPI
MS.gene025873	MS.gene013676	PPI
MS.gene025873	MS.gene29406	PPI
MS.gene025873	MS.gene09023	PPI
MS.gene025873	MS.gene021968	PPI
MS.gene025873	MS.gene054508	PPI
MS.gene025873	MS.gene025987	PPI
MS.gene28545	MS.gene025873	PPI
MS.gene025873	MS.gene95960	PPI
MS.gene025873	MS.gene053373	PPI
MS.gene025873	MS.gene043140	PPI
MS.gene025873	MS.gene041264	PPI
MS.gene025873	MS.gene35175	PPI
MS.gene008275	MS.gene025873	PPI
MS.gene025873	MS.gene042559	PPI
MS.gene025873	MS.gene035626	PPI
MS.gene025873	MS.gene46948	PPI
MS.gene025873	MS.gene44083	PPI
MS.gene025873	MS.gene29321	PPI
MS.gene025873	MS.gene65474	PPI
MS.gene025873	MS.gene018745	PPI
MS.gene025873	MS.gene025401	PPI
MS.gene025873	MS.gene57044	PPI
MS.gene025873	MS.gene015093	PPI
MS.gene025873	MS.gene04134	PPI
MS.gene025873	MS.gene24278	PPI
MS.gene025873	MS.gene000379	PPI
MS.gene025873	MS.gene34618	PPI
MS.gene025873	MS.gene032299	PPI
MS.gene025873	MS.gene009121	PPI
MS.gene025873	MS.gene08713	PPI
MS.gene025873	MS.gene70932	PPI
MS.gene025873	MS.gene09517	PPI
MS.gene025873	MS.gene87845	PPI
MS.gene025873	MS.gene57019	PPI
MS.gene025873	MS.gene32415	PPI
MS.gene72520	MS.gene025873	PPI
MS.gene025873	MS.gene85308	PPI
MS.gene025873	MS.gene62249	PPI
MS.gene025873	MS.gene58419	PPI
MS.gene025873	MS.gene046089	PPI
MS.gene025873	MS.gene053747	PPI
MS.gene025873	MS.gene28545	PPI
MS.gene025873	MS.gene69490	PPI
MS.gene025873	MS.gene021555	PPI
MS.gene025873	MS.gene05576	PPI
MS.gene025873	MS.gene044729	PPI
MS.gene025873	MS.gene96109	PPI
MS.gene025873	MS.gene008275	PPI
MS.gene025873	MS.gene60390	PPI
MS.gene025873	MS.gene48477	PPI
MS.gene025873	MS.gene027169	PPI
MS.gene025873	MS.gene55682	PPI
MS.gene016102	MS.gene025873	PPI
MS.gene025873	MS.gene81712	PPI
MS.gene46941	MS.gene025873	PPI
MS.gene025873	MS.gene019177	PPI
MS.gene025873	MS.gene05436	PPI
MS.gene025873	MS.gene038623	PPI
MS.gene025873	MS.gene005120	PPI
MS.gene025873	MS.gene055560	PPI
MS.gene025873	MS.gene05357	PPI
MS.gene025873	MS.gene024297	PPI
MS.gene025873	MS.gene35202	PPI
MS.gene025873	MS.gene22200	PPI
MS.gene025873	MS.gene057934	PPI
MS.gene025873	MS.gene37537	PPI
MS.gene025873	MS.gene024841	PPI
MS.gene93396	MS.gene025873	PPI
MS.gene025873	MS.gene20246	PPI
MS.gene025873	MS.gene069786	PPI
MS.gene025873	MS.gene42010	PPI
MS.gene025873	MS.gene025158	PPI
MS.gene025873	MS.gene049512	PPI
MS.gene025873	MS.gene40828	PPI
MS.gene025873	MS.gene27329	PPI
MS.gene025873	MS.gene87529	PPI
MS.gene025873	MS.gene41393	PPI
MS.gene025873	MS.gene30208	PPI
MS.gene025873	MS.gene97410	PPI
MS.gene025873	MS.gene56991	PPI
MS.gene025873	MS.gene010656	PPI
MS.gene025873	MS.gene016632	PPI
MS.gene56991	MS.gene025873	PPI
MS.gene025873	MS.gene013193	PPI
MS.gene025873	MS.gene40469	PPI
MS.gene025873	MS.gene96163	PPI
MS.gene025873	MS.gene65623	PPI
MS.gene20246	MS.gene025873	PPI
MS.gene025873	MS.gene012748	PPI
MS.gene025873	MS.gene063214	PPI
MS.gene025873	MS.gene66651	PPI
MS.gene025873	MS.gene02896	PPI
MS.gene025873	MS.gene71588	PPI
MS.gene025873	MS.gene72520	PPI
MS.gene025873	MS.gene61452	PPI
MS.gene025873	MS.gene05231	PPI
MS.gene025873	MS.gene03149	PPI
MS.gene025873	MS.gene037049	PPI
MS.gene025873	MS.gene042614	PPI
MS.gene025873	MS.gene026680	PPI
MS.gene025873	MS.gene051449	PPI
MS.gene025873	MS.gene042651	PPI
MS.gene025873	MS.gene59460	PPI
MS.gene025873	MS.gene019165	PPI
MS.gene025873	MS.gene81194	PPI
MS.gene025873	MS.gene060773	PPI
MS.gene025873	MS.gene66650	PPI
MS.gene69490	MS.gene025873	PPI
MS.gene025873	MS.gene038394	PPI
MS.gene025873	MS.gene09589	PPI
MS.gene015248	MS.gene025873	PPI
MS.gene025873	MS.gene019001	PPI
MS.gene025873	MS.gene024405	PPI
MS.gene025873	MS.gene067340	PPI
MS.gene025873	MS.gene021965	PPI
MS.gene025873	MS.gene018196	PPI
MS.gene025873	MS.gene038676	PPI
MS.gene025873	MS.gene53904	PPI
MS.gene025873	MS.gene24070	PPI
MS.gene025873	MS.gene83772	PPI
MS.gene025873	MS.gene73261	PPI
MS.gene013193	MS.gene025873	PPI
MS.gene025873	MS.gene059736	PPI
MS.gene08106	MS.gene025873	PPI
MS.gene025873	MS.gene85538	PPI
MS.gene025873	MS.gene94654	PPI
MS.gene025873	MS.gene26844	PPI
MS.gene025873	MS.gene64178	PPI
MS.gene025873	MS.gene055546	PPI
MS.gene025873	MS.gene55209	PPI
MS.gene025873	MS.gene96174	PPI
MS.gene025873	MS.gene074257	PPI
MS.gene025873	MS.gene04749	PPI
MS.gene025873	MS.gene073573	PPI
MS.gene025873	MS.gene28718	PPI
MS.gene025873	MS.gene44144	PPI
MS.gene025873	MS.gene060718	PPI
MS.gene025873	MS.gene63743	PPI
MS.gene025873	MS.gene016102	PPI
MS.gene025873	MS.gene055223	PPI
MS.gene025873	MS.gene068629	PPI
MS.gene025873	MS.gene08106	PPI
MS.gene025873	MS.gene004417	PPI
MS.gene025873	MS.gene009781	PPI
MS.gene025873	MS.gene031967	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene025873.t1	MTR_3g053920	62.656	241	63	4	1	229	55	280	4.12e-89	267
MS.gene025873.t1	MTR_4g103530	61.111	198	58	5	1	196	1	181	3.27e-74	226
MS.gene025873.t1	MTR_2g061500	86.667	75	10	0	1	75	55	129	4.43e-44	147
MS.gene025873.t1	MTR_8g463110	69.421	121	37	0	172	292	1	121	8.26e-40	147
MS.gene025873.t1	MTR_7g011710	69.412	85	26	0	1	85	1	85	5.90e-37	129
MS.gene025873.t1	MTR_0010s0370	43.902	123	63	4	5	123	57	177	3.67e-28	107
MS.gene025873.t1	MTR_0010s0350	43.902	123	63	4	5	123	57	177	3.67e-28	107
MS.gene025873.t1	MTR_6g033215	35.115	131	83	1	5	133	152	282	6.82e-24	99.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 90 sgRNAs with CRISPR-Local

Find 113 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CACTGAATCACATTGATTTC+TGG	0.138202	7.1:-534962	MS.gene025873:CDS
CATCGACCTTGTGGTGAAAT+TGG	0.244441	7.1:+535078	None:intergenic
ACGACTATCATAAGACTTAA+AGG	0.281382	7.1:+534866	None:intergenic
GTCTGACGGTGAATTTGTGC+TGG	0.326563	7.1:-534295	MS.gene025873:CDS
TTTGTGCTGGGTTTGGCTGC+TGG	0.328076	7.1:-534282	MS.gene025873:CDS
GTGATTCAGTGGGACAAAAC+TGG	0.330797	7.1:+534974	None:intergenic
GATGAAGGAAACATTGTAAT+AGG	0.345797	7.1:-534141	MS.gene025873:CDS
CGGTGAATTTGTGCTGGGTT+TGG	0.347218	7.1:-534289	MS.gene025873:CDS
ACTGAATCACATTGATTTCT+GGG	0.367380	7.1:-534961	MS.gene025873:CDS
CTCTTACCTTATTCCTCAAC+AGG	0.368499	7.1:-534339	MS.gene025873:CDS
CGTGGTACTGAAGCAGGAAT+AGG	0.371280	7.1:+534552	None:intergenic
GAATTACCTGTTGAGGAATA+AGG	0.376624	7.1:+534333	None:intergenic
CAAGTCCACGGTTTACCCTT+TGG	0.387847	7.1:-534936	MS.gene025873:CDS
TACCTTGACCTACTTTCTGT+TGG	0.393464	7.1:+534907	None:intergenic
AGGTGCTAGTGGTTCTTGTC+AGG	0.398289	7.1:-534367	MS.gene025873:CDS
GTAATTGTAACTTTCTTGCC+TGG	0.398943	7.1:+535128	None:intergenic
GTTTAGCCTTTGCAGCAACT+TGG	0.414818	7.1:+534397	None:intergenic
CCTTGTGGTGAAATTGGAAT+AGG	0.425107	7.1:+535084	None:intergenic
ACAAAACTGGGTACGACACC+AGG	0.437274	7.1:+534987	None:intergenic
CACGCCATTCTTGTTTCAAA+GGG	0.439548	7.1:+534790	None:intergenic
GCAAAGGCTAAACACGGTTC+AGG	0.440363	7.1:-534387	MS.gene025873:CDS
TAGTAACGGTTCCTTTGTTA+CGG	0.443190	7.1:-534760	MS.gene025873:intron
GCTGGTCCGTTTCTTAATGC+TGG	0.447601	7.1:-534264	MS.gene025873:CDS
ATCATTCTGTCGTTGAAACT+TGG	0.449924	7.1:+534468	None:intergenic
ATGTCTCGTGCTTGGAAGCC+AGG	0.453399	7.1:-535146	MS.gene025873:CDS
CAGAAAGTAGGTCAAGGTAT+AGG	0.453798	7.1:-534903	MS.gene025873:CDS
CTGGTTCCGATTTGAAGAAA+AGG	0.462538	7.1:-534218	MS.gene025873:CDS
CGCTGCCCAAGTTGCTGCAA+AGG	0.467656	7.1:-534403	MS.gene025873:CDS
TTCTGTTGGATGAAACCAAA+GGG	0.480894	7.1:+534921	None:intergenic
GAATCATCATTTGCTGCTGA+TGG	0.486408	7.1:-534165	MS.gene025873:CDS
TCTGACGGTGAATTTGTGCT+GGG	0.491645	7.1:-534294	MS.gene025873:CDS
CTGTCCCTTTGAAACAAGAA+TGG	0.493273	7.1:-534794	MS.gene025873:CDS
GTGGTACTGAAGCAGGAATA+GGG	0.493715	7.1:+534553	None:intergenic
TTATAGCAGTTAATTGACTC+TGG	0.497479	7.1:+534439	None:intergenic
ACACGCCATTCTTGTTTCAA+AGG	0.498805	7.1:+534789	None:intergenic
CAGAAATCAATGTGATTCAG+TGG	0.501051	7.1:+534963	None:intergenic
AATCATCATTTGCTGCTGAT+GGG	0.504180	7.1:-534164	MS.gene025873:CDS
AAATGCAACAGATGGTGGTC+AGG	0.508197	7.1:-534496	MS.gene025873:CDS
ACTTTCATATTGTCATGGAC+CGG	0.511440	7.1:-535016	MS.gene025873:CDS
ACCGTCAGACAGCAAGTTTG+AGG	0.513455	7.1:+534308	None:intergenic
GGTTTCATCCAACAGAAAGT+AGG	0.516836	7.1:-534915	MS.gene025873:CDS
ATGATTCTTCACCATTCTGA+AGG	0.517798	7.1:+534181	None:intergenic
TAGGTACAAATGTTAGTCAT+GGG	0.524354	7.1:-534122	MS.gene025873:CDS
TGAGGCTGAATTACCTGTTG+AGG	0.525618	7.1:+534326	None:intergenic
GAAGAAAAGGAAGAGAATGT+TGG	0.533755	7.1:-534205	MS.gene025873:CDS
ACAGCCACGGGCCTTCATCA+AGG	0.536062	7.1:+535045	None:intergenic
TTTAGCCTTTGCAGCAACTT+GGG	0.536452	7.1:+534398	None:intergenic
TTTGCTGCTGATGGGGATGA+AGG	0.538842	7.1:-534156	MS.gene025873:CDS
GTGAGCGTATGTCTCGTGCT+TGG	0.540385	7.1:-535154	None:intergenic
GCCTCAAACTTGCTGTCTGA+CGG	0.540508	7.1:-534309	MS.gene025873:CDS
CAAAGGGACAGTAACATCAA+TGG	0.541078	7.1:+534806	None:intergenic
AAGGATGATGTTGTTCCTGC+TGG	0.542430	7.1:-534522	MS.gene025873:CDS
GATTTCTGGGTGCAAGTCCA+CGG	0.545064	7.1:-534948	MS.gene025873:CDS
ATAGGTACAAATGTTAGTCA+TGG	0.550331	7.1:-534123	MS.gene025873:CDS
TGATAACTTTCATATTGTCA+TGG	0.552037	7.1:-535021	MS.gene025873:CDS
TGGCGTGTCCGTGCTAGTAA+CGG	0.557920	7.1:-534774	MS.gene025873:CDS
GTGTGTCGTGGTACTGAAGC+AGG	0.559588	7.1:+534546	None:intergenic
GTACGACACCAGGGGCAATC+CGG	0.560950	7.1:+534997	None:intergenic
GTCATGGACCGGATTGCCCC+TGG	0.563027	7.1:-535005	MS.gene025873:CDS
TCTGTTGCATTTCGACCAGC+AGG	0.566046	7.1:+534507	None:intergenic
TGATTCAGTGGGACAAAACT+GGG	0.566580	7.1:+534975	None:intergenic
CCTATTCCAATTTCACCACA+AGG	0.568495	7.1:-535084	MS.gene025873:CDS
GAAAGTTATCATACAGCCAC+GGG	0.569417	7.1:+535033	None:intergenic
TCACAGCCAGCATTAAGAAA+CGG	0.571104	7.1:+534258	None:intergenic
GCTGCACGCATCCTTGATGA+AGG	0.571959	7.1:-535056	MS.gene025873:CDS
CAAAGGAACCGTTACTAGCA+CGG	0.590633	7.1:+534766	None:intergenic
AGAATGTTGGCCCTTCAGAA+TGG	0.594097	7.1:-534192	MS.gene025873:CDS
GAATACGCATATAAGAGCTA+TGG	0.602257	7.1:+534835	None:intergenic
ATCATCATTTGCTGCTGATG+GGG	0.612968	7.1:-534163	MS.gene025873:CDS
TTCAGTACCACGACACACAA+AGG	0.613295	7.1:-534541	MS.gene025873:CDS
TTTAAAAGTAACCGTAACAA+AGG	0.615879	7.1:+534749	None:intergenic
ATCCAACAGAAAGTAGGTCA+AGG	0.616872	7.1:-534909	MS.gene025873:CDS
TGAAAGTTATCATACAGCCA+CGG	0.617024	7.1:+535032	None:intergenic
TGATTCTTCACCATTCTGAA+GGG	0.621952	7.1:+534182	None:intergenic
ACATCATCCTTTGTGTGTCG+TGG	0.623043	7.1:+534534	None:intergenic
AGGTACAAATGTTAGTCATG+GGG	0.624100	7.1:-534121	MS.gene025873:CDS
GGTACAAATGTTAGTCATGG+GGG	0.629053	7.1:-534120	MS.gene025873:CDS
AAACACGGTTCAGGTGCTAG+TGG	0.636490	7.1:-534378	MS.gene025873:CDS
CAAAACTGGGTACGACACCA+GGG	0.636645	7.1:+534988	None:intergenic
GCATCCTTGATGAAGGCCCG+TGG	0.637248	7.1:-535049	MS.gene025873:CDS
AGAAATCAATGTGATTCAGT+GGG	0.641223	7.1:+534964	None:intergenic
TTTCTGTTGGATGAAACCAA+AGG	0.643348	7.1:+534920	None:intergenic
GTTTCTTAATGCTGGCTGTG+AGG	0.660341	7.1:-534256	MS.gene025873:CDS
GCTGGTCGAAATGCAACAGA+TGG	0.671483	7.1:-534504	MS.gene025873:CDS
GGTCGAAATGCAACAGATGG+TGG	0.674246	7.1:-534501	MS.gene025873:CDS
GTACAAATGTTAGTCATGGG+GGG	0.677618	7.1:-534119	MS.gene025873:CDS
AAAACTGGGTACGACACCAG+GGG	0.705794	7.1:+534989	None:intergenic
TGCGTGCAGCATCGACCTTG+TGG	0.712710	7.1:+535069	None:intergenic
GTTGCTGCAAAGGCTAAACA+CGG	0.713576	7.1:-534393	MS.gene025873:CDS
TGAAACCAAAGGGTAAACCG+TGG	0.718993	7.1:+534931	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	ACTTTTAAATATGAAAAGCT+GGG	-	chr7.1:534518-534537	MS.gene025873:CDS	20.0%
!!!	TACTTTTAAATATGAAAAGC+TGG	-	chr7.1:534517-534536	MS.gene025873:CDS	20.0%
!	TGATAACTTTCATATTGTCA+TGG	-	chr7.1:534232-534251	MS.gene025873:CDS	25.0%
!	TTTAAAAGTAACCGTAACAA+AGG	+	chr7.1:534507-534526	None:intergenic	25.0%
	ACGACTATCATAAGACTTAA+AGG	+	chr7.1:534390-534409	None:intergenic	30.0%
	AGAAATCAATGTGATTCAGT+GGG	+	chr7.1:534292-534311	None:intergenic	30.0%
	ATAGGTACAAATGTTAGTCA+TGG	-	chr7.1:535130-535149	MS.gene025873:CDS	30.0%
	GATGAAGGAAACATTGTAAT+AGG	-	chr7.1:535112-535131	MS.gene025873:CDS	30.0%
	TAGGTACAAATGTTAGTCAT+GGG	-	chr7.1:535131-535150	MS.gene025873:CDS	30.0%
!	TCTCTTCCTTTTCTTCAAAT+CGG	+	chr7.1:535044-535063	None:intergenic	30.0%
!	TTATAGCAGTTAATTGACTC+TGG	+	chr7.1:534817-534836	None:intergenic	30.0%
!!	ACTGAATCACATTGATTTCT+GGG	-	chr7.1:534292-534311	MS.gene025873:CDS	30.0%
	ACTTTCATATTGTCATGGAC+CGG	-	chr7.1:534237-534256	MS.gene025873:CDS	35.0%
	AGGTACAAATGTTAGTCATG+GGG	-	chr7.1:535132-535151	MS.gene025873:CDS	35.0%
	ATCATTCTGTCGTTGAAACT+TGG	+	chr7.1:534788-534807	None:intergenic	35.0%
	ATGATTCTTCACCATTCTGA+AGG	+	chr7.1:535075-535094	None:intergenic	35.0%
	ATTCCAGCTCAAACAATTCT+GGG	+	chr7.1:534592-534611	None:intergenic	35.0%
	CAGAAATCAATGTGATTCAG+TGG	+	chr7.1:534293-534312	None:intergenic	35.0%
	GAAGAAAAGGAAGAGAATGT+TGG	-	chr7.1:535048-535067	MS.gene025873:CDS	35.0%
	GAATACGCATATAAGAGCTA+TGG	+	chr7.1:534421-534440	None:intergenic	35.0%
	GAATTACCTGTTGAGGAATA+AGG	+	chr7.1:534923-534942	None:intergenic	35.0%
	GTAATTGTAACTTTCTTGCC+TGG	+	chr7.1:534128-534147	None:intergenic	35.0%
	TAGTAACGGTTCCTTTGTTA+CGG	-	chr7.1:534493-534512	MS.gene025873:CDS	35.0%
	TGAAAGTTATCATACAGCCA+CGG	+	chr7.1:534224-534243	None:intergenic	35.0%
	TGATTCTTCACCATTCTGAA+GGG	+	chr7.1:535074-535093	None:intergenic	35.0%
	TTCTGTTGGATGAAACCAAA+GGG	+	chr7.1:534335-534354	None:intergenic	35.0%
	TTTCTGTTGGATGAAACCAA+AGG	+	chr7.1:534336-534355	None:intergenic	35.0%
!	AATCATCATTTGCTGCTGAT+GGG	-	chr7.1:535089-535108	MS.gene025873:CDS	35.0%
!	CAAGGTATAGGTAGCTTTTT+AGG	-	chr7.1:534362-534381	MS.gene025873:CDS	35.0%
!!	CACTGAATCACATTGATTTC+TGG	-	chr7.1:534291-534310	MS.gene025873:CDS	35.0%
	AAGCCAGTTACTCAAAGGAT+AGG	-	chr7.1:534647-534666	MS.gene025873:intron	40.0%
	ACCTTAAGCCAGTTACTCAA+AGG	-	chr7.1:534642-534661	MS.gene025873:intron	40.0%
	ATCCAACAGAAAGTAGGTCA+AGG	-	chr7.1:534344-534363	MS.gene025873:CDS	40.0%
	CAAAGGGACAGTAACATCAA+TGG	+	chr7.1:534450-534469	None:intergenic	40.0%
	CAGAAAGTAGGTCAAGGTAT+AGG	-	chr7.1:534350-534369	MS.gene025873:CDS	40.0%
	CCTATTCCAATTTCACCACA+AGG	-	chr7.1:534169-534188	MS.gene025873:CDS	40.0%
	CCTTGTGGTGAAATTGGAAT+AGG	+	chr7.1:534172-534191	None:intergenic	40.0%
	CTCTTACCTTATTCCTCAAC+AGG	-	chr7.1:534914-534933	MS.gene025873:CDS	40.0%
	GAAAGTTATCATACAGCCAC+GGG	+	chr7.1:534223-534242	None:intergenic	40.0%
	GAACCTATCCTTTGAGTAAC+TGG	+	chr7.1:534653-534672	None:intergenic	40.0%
	GATTCCAGCTCAAACAATTC+TGG	+	chr7.1:534593-534612	None:intergenic	40.0%
	GGTACAAATGTTAGTCATGG+GGG	-	chr7.1:535133-535152	MS.gene025873:CDS	40.0%
	GGTTTCATCCAACAGAAAGT+AGG	-	chr7.1:534338-534357	MS.gene025873:CDS	40.0%
	GTACAAATGTTAGTCATGGG+GGG	-	chr7.1:535134-535153	MS.gene025873:CDS	40.0%
	TACCTTGACCTACTTTCTGT+TGG	+	chr7.1:534349-534368	None:intergenic	40.0%
	TCACAGCCAGCATTAAGAAA+CGG	+	chr7.1:534998-535017	None:intergenic	40.0%
	TGATTCAGTGGGACAAAACT+GGG	+	chr7.1:534281-534300	None:intergenic	40.0%
	TTTGAGCTGGAATCTGATGA+CGG	-	chr7.1:534599-534618	MS.gene025873:intron	40.0%
!	ACACGCCATTCTTGTTTCAA+AGG	+	chr7.1:534467-534486	None:intergenic	40.0%
!	ATCATCATTTGCTGCTGATG+GGG	-	chr7.1:535090-535109	MS.gene025873:CDS	40.0%
!	CACGCCATTCTTGTTTCAAA+GGG	+	chr7.1:534466-534485	None:intergenic	40.0%
!	CTGGTTCCGATTTGAAGAAA+AGG	-	chr7.1:535035-535054	MS.gene025873:CDS	40.0%
!	GAATCATCATTTGCTGCTGA+TGG	-	chr7.1:535088-535107	MS.gene025873:CDS	40.0%
!	GAGGAGTCTCATGATTTTAC+TGG	-	chr7.1:535016-535035	MS.gene025873:CDS	40.0%
!	TCCTTTGAGTAACTGGCTTA+AGG	+	chr7.1:534646-534665	None:intergenic	40.0%
!	TTGAGTAACTGGCTTAAGGT+AGG	+	chr7.1:534642-534661	None:intergenic	40.0%
!	TTTAGCCTTTGCAGCAACTT+GGG	+	chr7.1:534858-534877	None:intergenic	40.0%
!!	CTGTCCCTTTGAAACAAGAA+TGG	-	chr7.1:534459-534478	MS.gene025873:CDS	40.0%
!!	TGATGACGGTGTTCGTAATT+GGG	-	chr7.1:534613-534632	MS.gene025873:intron	40.0%
	AAGGATGATGTTGTTCCTGC+TGG	-	chr7.1:534731-534750	MS.gene025873:intron	45.0%
	ACATCATCCTTTGTGTGTCG+TGG	+	chr7.1:534722-534741	None:intergenic	45.0%
	AGAATGTTGGCCCTTCAGAA+TGG	-	chr7.1:535061-535080	MS.gene025873:CDS	45.0%
	CAAAGGAACCGTTACTAGCA+CGG	+	chr7.1:534490-534509	None:intergenic	45.0%
	CATCGACCTTGTGGTGAAAT+TGG	+	chr7.1:534178-534197	None:intergenic	45.0%
	GTGATTCAGTGGGACAAAAC+TGG	+	chr7.1:534282-534301	None:intergenic	45.0%
	GTTGCTGCAAAGGCTAAACA+CGG	-	chr7.1:534860-534879	MS.gene025873:CDS	45.0%
	TCTGACGGTGAATTTGTGCT+GGG	-	chr7.1:534959-534978	MS.gene025873:CDS	45.0%
	TGAAACCAAAGGGTAAACCG+TGG	+	chr7.1:534325-534344	None:intergenic	45.0%
	TGAGGCTGAATTACCTGTTG+AGG	+	chr7.1:534930-534949	None:intergenic	45.0%
	TTCAGTACCACGACACACAA+AGG	-	chr7.1:534712-534731	MS.gene025873:intron	45.0%
!	AAATGCAACAGATGGTGGTC+AGG	-	chr7.1:534757-534776	MS.gene025873:intron	45.0%
!	GATGACGGTGTTCGTAATTG+GGG	-	chr7.1:534614-534633	MS.gene025873:intron	45.0%
!	GTGGTACTGAAGCAGGAATA+GGG	+	chr7.1:534703-534722	None:intergenic	45.0%
!	GTTTAGCCTTTGCAGCAACT+TGG	+	chr7.1:534859-534878	None:intergenic	45.0%
!	GTTTCTTAATGCTGGCTGTG+AGG	-	chr7.1:534997-535016	MS.gene025873:CDS	45.0%
!!	CTGATGACGGTGTTCGTAAT+TGG	-	chr7.1:534612-534631	MS.gene025873:intron	45.0%
!!!	AGCTGGGTGTTTTTTGTCAC+AGG	-	chr7.1:534534-534553	MS.gene025873:CDS	45.0%
!!!	GGTGTTTTTTGTCACAGGTG+CGG	-	chr7.1:534539-534558	MS.gene025873:CDS	45.0%
	AAAACTGGGTACGACACCAG+GGG	+	chr7.1:534267-534286	None:intergenic	50.0%
	AAACACGGTTCAGGTGCTAG+TGG	-	chr7.1:534875-534894	MS.gene025873:CDS	50.0%
	ACAAAACTGGGTACGACACC+AGG	+	chr7.1:534269-534288	None:intergenic	50.0%
	ACCGTCAGACAGCAAGTTTG+AGG	+	chr7.1:534948-534967	None:intergenic	50.0%
	AGGTGCTAGTGGTTCTTGTC+AGG	-	chr7.1:534886-534905	MS.gene025873:CDS	50.0%
	CAAAACTGGGTACGACACCA+GGG	+	chr7.1:534268-534287	None:intergenic	50.0%
	CAAGTCCACGGTTTACCCTT+TGG	-	chr7.1:534317-534336	MS.gene025873:CDS	50.0%
	CGGTGAATTTGTGCTGGGTT+TGG	-	chr7.1:534964-534983	MS.gene025873:CDS	50.0%
	GCAAAGGCTAAACACGGTTC+AGG	-	chr7.1:534866-534885	MS.gene025873:CDS	50.0%
	GCTGGTCCGTTTCTTAATGC+TGG	-	chr7.1:534989-535008	MS.gene025873:CDS	50.0%
	GCTGGTCGAAATGCAACAGA+TGG	-	chr7.1:534749-534768	MS.gene025873:intron	50.0%
	GGTCGAAATGCAACAGATGG+TGG	-	chr7.1:534752-534771	MS.gene025873:intron	50.0%
	GTCTGACGGTGAATTTGTGC+TGG	-	chr7.1:534958-534977	MS.gene025873:CDS	50.0%
	GTGCCCAGAATTGTTTGAGC+TGG	-	chr7.1:534586-534605	MS.gene025873:intron	50.0%
	GTTCTGCTGCAACCAACTGA+TGG	-	chr7.1:534669-534688	MS.gene025873:intron	50.0%
	TCTGTTGCATTTCGACCAGC+AGG	+	chr7.1:534749-534768	None:intergenic	50.0%
	TGCAACCAACTGATGGCTAC+AGG	-	chr7.1:534676-534695	MS.gene025873:intron	50.0%
	TTTGCTGCTGATGGGGATGA+AGG	-	chr7.1:535097-535116	MS.gene025873:CDS	50.0%
!	CGTGGTACTGAAGCAGGAAT+AGG	+	chr7.1:534704-534723	None:intergenic	50.0%
!	GATTTCTGGGTGCAAGTCCA+CGG	-	chr7.1:534305-534324	MS.gene025873:CDS	50.0%
!!	GCCTCAAACTTGCTGTCTGA+CGG	-	chr7.1:534944-534963	MS.gene025873:CDS	50.0%
	AGGGTCCTGTAGCCATCAGT+TGG	+	chr7.1:534684-534703	None:intergenic	55.0%
	ATGTCTCGTGCTTGGAAGCC+AGG	-	chr7.1:534107-534126	MS.gene025873:CDS	55.0%
	GCTGCACGCATCCTTGATGA+AGG	-	chr7.1:534197-534216	MS.gene025873:CDS	55.0%
	TGGCGTGTCCGTGCTAGTAA+CGG	-	chr7.1:534479-534498	MS.gene025873:CDS	55.0%
	TGTCACAGGTGCGGTCTGTT+GGG	-	chr7.1:534548-534567	MS.gene025873:CDS	55.0%
	TTGTCACAGGTGCGGTCTGT+TGG	-	chr7.1:534547-534566	MS.gene025873:CDS	55.0%
	TTTGTGCTGGGTTTGGCTGC+TGG	-	chr7.1:534971-534990	MS.gene025873:CDS	55.0%
!	GTGTGTCGTGGTACTGAAGC+AGG	+	chr7.1:534710-534729	None:intergenic	55.0%
	ACAGCCACGGGCCTTCATCA+AGG	+	chr7.1:534211-534230	None:intergenic	60.0%
	CGCTGCCCAAGTTGCTGCAA+AGG	-	chr7.1:534850-534869	MS.gene025873:CDS	60.0%
	GCATCCTTGATGAAGGCCCG+TGG	-	chr7.1:534204-534223	MS.gene025873:CDS	60.0%
	GTACGACACCAGGGGCAATC+CGG	+	chr7.1:534259-534278	None:intergenic	60.0%
!!	TGCGTGCAGCATCGACCTTG+TGG	+	chr7.1:534187-534206	None:intergenic	60.0%
	GTCATGGACCGGATTGCCCC+TGG	-	chr7.1:534248-534267	MS.gene025873:CDS	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.1	gene	534107	535168	534107	ID=MS.gene025873
chr7.1	mRNA	534107	535168	534107	ID=MS.gene025873.t1;Parent=MS.gene025873
chr7.1	exon	534761	535168	534761	ID=MS.gene025873.t1.exon1;Parent=MS.gene025873.t1
chr7.1	CDS	534761	535168	534761	ID=cds.MS.gene025873.t1;Parent=MS.gene025873.t1
chr7.1	exon	534107	534577	534107	ID=MS.gene025873.t1.exon2;Parent=MS.gene025873.t1
chr7.1	CDS	534107	534577	534107	ID=cds.MS.gene025873.t1;Parent=MS.gene025873.t1

Gene Sequence

>MS.gene025873

ATGTCTCGTGCTTGGAAGCCAGGCAAGAAAGTTACAATTACTAAGTCTGTGTCTGATCATTACCTATTCCAATTTCACCACAAGGTCGATGCTGCACGCATCCTTGATGAAGGCCCGTGGCTGTATGATAACTTTCATATTGTCATGGACCGGATTGCCCCTGGTGTCGTACCCAGTTTTGTCCCACTGAATCACATTGATTTCTGGGTGCAAGTCCACGGTTTACCCTTTGGTTTCATCCAACAGAAAGTAGGTCAAGGTATAGGTAGCTTTTTAGGTACCTTTAAGTCTTATGATAGTCGTAACTCAATCCATAGCTCTTATATGCGTATTCGTGTTGCCATTGATGTTACTGTCCCTTTGAAACAAGAATGGCGTGTCCGTGCTAGTAACGGTTCCTTTGTTACGGTTACTTTTAAATATGAAAAGCTGGGTGTTTTTTGTCACAGGTGCGGTCTGTTGGGACATACTGACAAAGTGTGCCCAGAATTGTTTGAGCTGGAATCTGATGACGGTGTTCGTAATTGGGGCGCCTACCTTAAGCCAGTTACTCAAAGGATAGGTTCTGCTGCAACCAACTGATGGCTACAGGACCCTATTCCTGCTTCAGTACCACGACACACAAAGGATGATGTTGTTCCTGCTGGTCGAAATGCAACAGATGGTGGTCAGGTTGTACCAAGTTTCAACGACAGAATGATTGCTGTCCAGAGTCAATTAACTGCTATAAAACATGATGTTCTCGCTGCCCAAGTTGCTGCAAAGGCTAAACACGGTTCAGGTGCTAGTGGTTCTTGTCAGGTGCATCTCTTACCTTATTCCTCAACAGGTAATTCAGCCTCAAACTTGCTGTCTGACGGTGAATTTGTGCTGGGTTTGGCTGCTGGTCCGTTTCTTAATGCTGGCTGTGAGGAGTCTCATGATTTTACTGGTTCCGATTTGAAGAAAAGGAAGAGAATGTTGGCCCTTCAGAATGGTGAAGAATCATCATTTGCTGCTGATGGGGATGAAGGAAACATTGTAATAGGTACAAATGTTAGTCATGGGGGGATGTTGAAATGA

Protein sequence

>MS.gene025873.t1

MSRAWKPGKKVTITKSVSDHYLFQFHHKVDAARILDEGPWLYDNFHIVMDRIAPGVVPSFVPLNHIDFWVQVHGLPFGFIQQKVGQGIGSFLGTFKSYDSRNSIHSSYMRIRVAIDVTVPLKQEWRVRASNGSFVTDPIPASVPRHTKDDVVPAGRNATDGGQVVPSFNDRMIAVQSQLTAIKHDVLAAQVAAKAKHGSGASGSCQVHLLPYSSTGNSASNLLSDGEFVLGLAAGPFLNAGCEESHDFTGSDLKKRKRMLALQNGEESSFAADGDEGNIVIGTNVSHGGMLK